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1.
Nucleic Acids Res ; 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31691823

RESUMO

Knowledge of therapeutic targets and early drug candidates is useful for improved drug discovery. In particular, information about target regulators and the patented therapeutic agents facilitates research regarding druggability, systems pharmacology, new trends, molecular landscapes, and the development of drug discovery tools. To complement other databases, we constructed the Therapeutic Target Database (TTD) with expanded information about (i) target-regulating microRNAs and transcription factors, (ii) target-interacting proteins, and (iii) patented agents and their targets (structures and experimental activity values if available), which can be conveniently retrieved and is further enriched with regulatory mechanisms or biochemical classes. We also updated the TTD with the recently released International Classification of Diseases ICD-11 codes and additional sets of successful, clinical trial, and literature-reported targets that emerged since the last update. TTD is accessible at http://bidd.nus.edu.sg/group/ttd/ttd.asp. In case of possible web connectivity issues, two mirror sites of TTD are also constructed (http://db.idrblab.org/ttd/ and http://db.idrblab.net/ttd/).

2.
Sensors (Basel) ; 19(22)2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31726702

RESUMO

The resolution of contact location is important in many applications in robotics and automation. This is generally done by using an array of contact or tactile receptors, which increases cost and complexity as the required resolution or area is increased. Tactile sensors have also been developed using a continuous deformable medium between the contact and the receptors, which allows few receptors to interpolate the information among them, avoiding the weakness highlighted in the former approach. The latter is generally used to measure contact force intensity or magnitude but rarely used to identify the contact locations. This paper presents a systematic design and characterisation procedure for magnetic-based soft tactile sensors (utilizing the latter approach with the deformable contact medium) with the goal of locating the contact force location. This systematic procedure provides conditions under which design parameters can be selected, supported by a selected machine learning algorithm, to achieve the desired performance of the tactile sensor in identifying the contact location. An illustrative example, which combines a particular sensor configuration (magnetic hall effect sensor as the receptor, a selected continuous medium and a selected sensing resolution) and a specific data-driven algorithm, is used to illustrate the proposed design procedure. The results of the illustrative example design demonstrates the efficacy of the proposed design procedure and the proposed sensing strategy in identifying a contact location. The resulting sensor is also tested on a robotic hand (Allegro Hand, SimLab Co) to demonstrate its application in real-world scenarios.

3.
Zhongguo Fei Ai Za Zhi ; 22(10): 633-638, 2019 Oct 20.
Artigo em Chinês | MEDLINE | ID: mdl-31650945

RESUMO

Immune checkpoint inhibitors (ICIs) can cause adverse reactions in the nervous system, with the incidence rate ranging from 0.1% to 12%, and 80% occurring within the first 4 months of ICI application. It can cause lesions in various parts of the nervous system, including aseptic meningitis, meningoencephalitis, necrotizing encephalitis, brainstem encephalitis, transverse myelitis and other central nervous system diseases. It can also cause cranial peripheral neuropathy, multifocal radicular neuropathy, Guillain-Barre syndrome, spinal radicular neuropathy and myasthenia gravis, myopathy, etc. For these complications of the nervous system, diagnosis could be made by sufficient collection of disease manifestations combined with imaging, cerebrospinal fluid examinations, electro-encephalogram or electro myography to exclude infection or tumor progression. In the treatment of severe cases, ICIs should be discontinued and treated with high doses of glucocorticoid or gamma globulin with systemic support. After neurological adverse reactions, the prognosis of severe cases is poor.

4.
J Org Chem ; 84(22): 14498-14507, 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31524391

RESUMO

Functional molecules with aggregation-induced emission (AIE) have lately received the most attention due to their versatile functions in many fields. In this work, tetraphenylethene (TPE) as one of the most discussed AIE system was installed on different fluorophores including dansyl, naphthalimide, 4-nitro-1,2,3-benzoxadiazole (NBD), borondipyrromethene, and hemicyanine to afford a series of new fluorescent dyes, whose spectra covered the fluorescence region of the visible to near-infrared emission. Especially, these tetraphenylethene-containing compounds all exhibited AIE behavior. In solid state, they presented multiple colors including blue, orange, red, crimson, and NIR (near-infrared emission). It was worth mentioning that the dansyl- and NBD-coated TPEs exhibited obvious mechanoresponsive luminescence phenomena. Moreover, these AIE-based TPEs displayed a good bioimaging performance in living cells. This work will be helpful for designing functional AIE dyes with different fluorescence emission.

5.
Opt Express ; 27(15): 20942-20957, 2019 Jul 22.
Artigo em Inglês | MEDLINE | ID: mdl-31510181

RESUMO

A broadband monolithic spatial heterodyne spectrometer (SHS) system is built for measuring nitrogen dioxide in the atmosphere based on our newly developed fabrication technique. This system is calibrated and tested with Hg, Kr and Xe lamps, as well as monochromator output illuminated by a high-voltage Xe lamp (as a white light source). The obtained overall efficiency profile presents an effective spectral range of 425-495 nm (when the efficiency values are greater than 40%). The maximum fringe visibility is ~0.85. The measured instrumental line shape function gives an actual spectral resolution of ~0.073 nm. The effect of phase distortion of this 2-D SHS system can be neglected. Direct solar-irradiance spectra in the NO2 absorption band were measured with the SHS system. The measured spectra are consistent with the results simulated by Modtran6 within the SHS spectral range. The vertical column contents of NO2, VC(NO2), derived from the SHS data by the direct sun - differential optical absorption spectroscopy (DS-DOAS) method coincide closely with the simultaneously acquired (OMI) satellite data.

6.
Redox Biol ; 26: 101287, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31386965

RESUMO

The basic pathophysiological mechanisms underlying septic cardiomyopathy have not yet been completely clarified. Disease-specific treatments are lacking, and care is still based on supportive modalities. The aim of our study was to assess the protective effects of melatonin on septic cardiomyopathy, with a focus on the interactions between receptor-interacting protein kinase 3 (Ripk3), the mitochondria, endoplasmic reticulum (ER) and cytoskeletal degradation in cardiomyocytes. Ripk3 expression was increased in heart samples challenged with LPS, followed by myocardial inflammation, cardiac dysfunction, myocardial breakdown and cardiomyocyte death. The melatonin treatment attenuated septic myocardial injury in a comparable manner to the genetic depletion of Ripk3. Molecular investigations revealed that Ripk3 intimately regulated mitochondrial function, ER stress, cytoskeletal homeostasis and cardioprotective signaling pathways. Melatonin-mediated inhibition of Ripk3 improved mitochondrial bioenergetics, reduced mitochondria-initiated oxidative damage, sustained mitochondrial dynamics, ameliorated ER stress, normalized calcium recycling, and activated cardioprotective signaling pathways (including AKT, ERK and AMPK) in cardiomyocytes. Interestingly, Ripk3 overexpression mediated resistance to melatonin therapy following the infection of LPS-treated hearts with an adenovirus expressing Ripk3. Altogether, our findings identify Ripk3 upregulation as a novel risk factor for the development of sepsis-related myocardial injury, and melatonin restores the physiological functions of the mitochondria, ER, contractile cytoskeleton and cardioprotective signaling pathways. Additionally, our data also reveal a new, potentially therapeutic mechanism by which melatonin protects the heart from sepsis-mediated dysfunction, possibly by targeting Ripk3.

7.
IEEE Int Conf Rehabil Robot ; 2019: 83-88, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31374611

RESUMO

Wool harvesting remains an important industry in Australia, but its workers suffer from extreme rates of injury, in particular, the lower back injuries. Reducing injuries in sheep shearing could be as simple as extending shearer rest periods between sheep, but the effect of this has not previously been studied. The lumbar flexion-relaxation phenomenon is present in sheep shearing and the onset angle of this phenomenon can provide insight into lower back injury risk. The increase in the onset angle of lumbar flexion-relaxation over several work-rest periods for a simulated sheep shearing task is studied. The rate of increase in the onset angle of lumbar flexion-relaxation was higher when shorter breaks were taken for all participants at least unilaterally, indicating that longer rest breaks could reduce back injury risk. Due to the constraints of the sheep shearing occupation, this type of intervention is better suited to learner and novice shearers. Assistive robotic devices would be more suited to reduce injuries in expert shearers, and some insight is provided for the application of these within sheep shearing. Further study of this phenomenon in sheep shearing could provide additional insight to developing an assistive device that could reduce injury.

8.
IEEE Int Conf Rehabil Robot ; 2019: 977-982, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-31374756

RESUMO

Movement patterns are commonly disrupted after a neurological incident. The correction and recovery of these movement patterns is part of therapeutic practice, and should be considered in the development of robotic device control strategies. This is an area which has limited exploration in rehabilitation robotics literature. This work presents a new strategy aiming at influencing the cost associated with a movement, based on the principle of optimal motor control. This approach is unique, in that it does not directly modify the movement pattern, but instead encourages this altered movement. This 'Indirect Shaping Control' is applied in a preliminary experiment using an end-effector based device with 5 healthy subjects. The study concludes that such an approach may encourage changes in movement patterns which do persist to out-of-robot reaching actions, but this was not consistent over all subjects and further experiments are required.

9.
Anal Chem ; 91(17): 11343-11348, 2019 Sep 03.
Artigo em Inglês | MEDLINE | ID: mdl-31386811

RESUMO

Molecular probes suitable for different fluorescence imaging technologies can meet the requirements of different scientific research in biological applications. In this work, a naphthalimide-cyanine-based sulfonamide was used to specifically visualize the glutathione of mouse tissues with a two-photon manner for the naphthalimide moiety and a near-infrared manner for the cyanine moiety, respectively. The results showed that this probe served as a dual-model tissue-imaging agent for visualization of glutathione with around 200 µm imaging depth in a two-photon manner and 120 µm imaging depth in a near-infrared manner, which provided a model for tissue imaging in the visible and near-infrared channels.

10.
Int J Biol Macromol ; 139: 1123-1132, 2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31394150

RESUMO

Echinacea purpurea polysaccharide (EPP) was obtained by modern separation technology and sulfated EPP (sEPP) was prepared by sulfation modification. The immunological effects of EPP and sEPP were compared on chicken bone marrow-derived dendritic cells (chBM-DCs). The results showed that the surface marker expression of CD11c and CD80 was increased after chBM-DCs were cultured with three dosage of sEPP, especially in sEPPM group. Three dosage of sEPP, EPPL and LPS could significantly enhance the effects of chBM-DCs on the proliferation of allogenic mixed lymphocytes. After chBM-DCs treatment with EPP or sEPP in vitro, the levels of IL-2 of sEPPH and EPPM groups were significantly higher than those of LPS group (P < 0.05). All sEPP and EPP groups could enhance the level of IFN-γ and down-regulated the level of IL-4 and IL-10. Results indicated that both sEPP and EPP had immunoregulatory effects on chBM-DCs, sEPP possessed better immunoregulatory effects as compared with EPP.

11.
Cell Stress Chaperones ; 24(5): 991-1003, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31388827

RESUMO

Cardiac microvascular ischemia-reperfusion (IR) injury has been a neglected topic in recent decades. In the current study, we investigated the mechanism underlying microvascular IR injury, with a focus on mitochondrial homeostasis. We also explored the protective role of tanshinone IIA (Tan IIA) in microvascular protection in the context of IR injury. Through animal studies and cell experiments, we demonstrated that IR injury mediated microvascular wall destruction, lumen stenosis, perfusion defects, and cardiac microvascular endothelial cell (CMEC) apoptosis via inducing mitochondrial damage. In contrast, Tan IIA administration had the ability to sustain CMEC viability and microvascular homeostasis, finally attenuating microvascular IR injury. Function studies have confirmed that the SIRT1/PGC1α pathway is responsible for the microvascular protection from the Tan IIA treatment. SIRT1 activation by Tan IIA sustained the mitochondrial potential, alleviated the mitochondrial pro-apoptotic factor leakage, reduced the mPTP opening, and blocked mitochondrial apoptosis, providing a survival advantage for CMECs and preserving microvascular structure and function. By comparison, inhibiting SIRT1 abrogated the beneficial effects of Tan IIA on mitochondrial function, CMEC survival, and microvascular homeostasis. Collectively, this study indicated that Tan IIA should be considered a microvascular-protective drug that alleviates acute cardiac microcirculation IR injury via activating the SIRT1/PGC1α pathway and thereby blocking mitochondrial damage.

12.
PLoS One ; 14(7): e0219979, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31335886

RESUMO

The halophyte Suaeda salsa displayed strong resistance to salinity. Up to date, molecular mechanisms underlying tolerance of S. salsa to salinity have not been well understood. In the present study, S. salsa seedlings were treated with 30‰ salinity and then leaves and roots were subjected to Illumina sequencing. Compared with the control, 68,599 and 77,250 unigenes were significantly differentially expressed in leaves and roots in saline treatment, respectively. KEGG enrichment analyses indicated that photosynthesis process, carbohydrate, lipid and amino acid metabolisms were all downregulated in saline treatment, which should inhibit growth of S. salsa. Expression levels of Na+/H+ exchanger, V-H+ ATPase, choline monooxygenase, potassium and chloride channels were upregulated in saline treatment, which could relieve reduce over-accumulation of Na+ and Cl-. Fe-SOD, glutathione, L-ascorbate and flavonoids function as antioxidants in plants. Genes in relation to them were all upregulated, suggesting that S. salsa initiated various antioxidant mechanisms to tolerate high salinity. Besides, plant hormones, especially auxin, ethylene and jasmonic acid signaling transduction pathways were all upregulated in response to saline treatment, which were important to gene regulations of ion transportation and antioxidation. These changes might comprehensively contribute to tolerance of S. salsa to salinity. Overall, the present study provided new insights to understand the mechanisms underlying tolerance to salinity in halophytes.

13.
J Mol Histol ; 50(5): 405-415, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31256303

RESUMO

Viral myocarditis has been found to be one of the leading causes of sudden death in young adults. However, no effective drugs have been developed to intervene the progression of myocarditis. Accordingly, the present study is carried out to explore the protective role played by melatonin in the setting of viral myocarditis with a focus on Mst1-Hippo pathway, mitochondrial dysfunction and ER stress. Cardiac function was determined via echocardiographic examination. Mitochondrial function and ER stress were detected via ELISA, western blots, and immunofluorescence. Our data demonstrated that virus injection induced cardiac dysfunction as evidenced by reduced contractile function in myocardium. Besides, LDH release assay and western blotting analysis demonstrated that cardiomyocyte death was activated by virus injection. Interestingly, melatonin treatment improved cardiac function and repressed virus-mediated cardiomyocyte apoptosis. At the molecular levels, mitochondrial dysfunction was induced by virus infection, as indicated by mitochondrial membrane potential reduction, mPTP opening rate elevation and caspase-9-related apoptosis activation. Besides, ER stress parameters were also elevated in virus-treated cardiomyocytes. Interestingly, melatonin treatment maintained mitochondrial dysfunction and repressed ER stress. To the end, we found that Mst1 was upregulated by virus infection; this effect was attenuated through supplementation with melatonin. However, Mst1 overexpression reduced the beneficial impact exerted by melatonin on cardiomyocyte viability, mitochondrial function and ER homeostasis. Our study illustrated that melatonin treatment attenuated viral myocarditis via sustaining cardiomyocyte viability, repressing mitochondrial dysfunction and inhibiting ER stress in a manner dependent on Mst1 inhibition.

14.
Curr Pharm Des ; 25(18): 2060-2070, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31284854

RESUMO

BACKGROUND: Sepsis and septic shock are known to prompt multiple organ failure including cardiac contractile dysfunction, which is typically referred to as septic cardiomyopathy. Among various theories postulated for the etiology of septic cardiomyopathy, mitochondrial injury (both morphology and function) in the heart is perceived as the main culprit for reduced myocardial performance and ultimately heart failure in the face of sepsis. METHODS: Over the past decades, ample of experimental and clinical work have appeared, focusing on myocardial mitochondrial changes and related interventions in septic cardiomyopathy. RESULTS AND CONCLUSION: Here we will briefly summarize the recent experimental and clinical progress on myocardial mitochondrial morphology and function in sepsis, and discuss possible underlying mechanisms, as well as the contemporary interventional options.

15.
IEEE Trans Cybern ; 2019 Jun 17.
Artigo em Inglês | MEDLINE | ID: mdl-31217140

RESUMO

Synergies have been adopted in prosthetic limb applications to reduce the complexity of design, but typically involve a single synergy setting for a population and ignore individual preference or adaptation capacity. However, personalization of the synergy setting is necessary for the effective operation of the prosthetic device. Two major challenges hinder the personalization of synergies in human-prosthesis interfaces (HPIs). The first is related to the process of human motor adaptation and the second to the variation in motor learning dynamics of individuals. In this paper, a systematic personalization of kinematic synergies for HPIs using online measurements from each individual is proposed. The task of reaching using the upper limb is described by an objective function and the interface is parameterized by a kinematic synergy. Consequently, personalizing the interface for a given individual can be formulated as finding an optimal personalized parameter. A structure to model the observed motor behavior that allows for the personalized traits of motor preference and motor learning is proposed, and subsequently used in an online optimization scheme to identify the synergies for an individual. The knowledge of the common features contained in the model enables online adaptation of the HPI to happen concurrently to human motor adaptation without the need to retune the personalization algorithm for each individual. Human-in-the-loop experimental results with able-bodied subjects, performed in a virtual reality environment to emulate amputation and prosthesis use, show that the proposed personalization algorithm was effective in obtaining optimal synergies with a fast uniform convergence speed across a group of individuals.

16.
Ophthalmic Plast Reconstr Surg ; 35(6): 623-627, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31219942

RESUMO

OBJECTIVE: Prolapse of silicone tubes is one of the most common complications after bicanalicular intubation of the lacrimal system. This study describes a new method of repositioning prolapsed silicone tubes by using a suture-probe. METHODS: This study was a retrospective chart review of 12 patients who experienced complete silicone tube prolapse after bicanalicular intubation. The silicone tube was repositioned by using a suture-probe; a double-stranded suture was placed in the lacrimal duct with a lacrimal probe with a hole in the front blunt tip, then interlocked with the prolapsed silicone tube. Subsequently, the suture loop was slowly stretched from the nasal cavity, and the silicone tube was repositioned. The silicone tube at the medial canthus was cut and loosened, then pulled out of the nasal cavity. RESULTS: In all 12 cases, the silicone tube was restored with 100% success by using the suture-probe. Three patients were extubated immediately after the repositioning. For another 9 patients with an indwelling tube, extubation was performed after 3-4 months, and no patients experienced further tube dislocation during that time. CONCLUSIONS: The suture-probe technique is a simple, low cost, and minimally traumatic method for repositioning a prolapsed silicone tube after bicanalicular intubation of the lacrimal system.A new technique, the suture-probe with thread interlocking the silicone tube ring can reposition the prolapsed silicone tube in lacrimal sac.

17.
J Cell Mol Med ; 23(7): 4640-4652, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31104354

RESUMO

Hypertension contributes to the high cardiac morbidity and mortality. Although oxidative stress plays an essential role in hypertensive heart diseases, the mechanism remains elusive. Transgenic mice with cardiac overexpression of metallothionein, a heavy metal-binding scavenger, were challenged with NG -nitro-L-arginine methyl ester (L-NAME) for 14 days prior to measurement of myocardial contractile and intracellular Ca2+ anomalies as well as cell signalling mechanisms using Western blot and immunofluorescence analysis. L-NAME challenge elicited hypertension, macrophage infiltration, oxidative stress, inflammation and cardiac dysfunction manifested as increased proinflammatory macrophage marker F4/80, interleukin-1ß (IL-1ß), intracellular O 2 - production, LV end systolic and diastolic diameters as well as depressed fractional shortening. L-NAME treatment reduced mitochondrial membrane potential (MMP), impaired cardiomyocyte contractile and intracellular Ca2+ properties as evidenced by suppressed peak shortening, maximal velocity of shortening/relengthening, rise in intracellular Ca2+ , along with elevated baseline and peak intracellular Ca2+ . These unfavourable mechanical changes and decreased MMP (except blood pressure and macrophage infiltration) were alleviated by overexpression of metallothionein. Furthermore, the apoptosis markers including BAD, Bax, Caspase 9, Caspase 12 and cleaved Caspase 3 were up-regulated while the anti-apoptotic marker Bcl-2 was decreased by L-NAME treatment. Metallothionein transgene reversed L-NAME-induced changes in Bax, Bcl-2, BAD phosphorylation, Caspase 9, Caspase 12 and cleaved Caspase 3. Our results suggest that metallothionein protects against L-NAME-induced myocardial contractile anomalies in part through inhibition of apoptosis.

18.
Biochim Biophys Acta Mol Basis Dis ; 1865(7): 1898-1904, 2019 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-31109454

RESUMO

The advance in medical technology and healthcare has dramatically improved the average human lifespan. One of the consequences for longevity is the high prevalence of aging-related chronic disorders such as cardiovascular diseases, cancer and metabolic abnormalities. As the composition of aging population is raising in western countries, heart failure remains the number one cause of death with a more severe impact in the elderly. Obesity and aging are the most critical risk factors for increased susceptibility to heart failure in developing and developed countries. Numerous population-based and experimental data have depicted a close relationship between the age-related diseases and obesity. There is an overall agreement that obesity is causally linked to the development of cardiovascular disorders and severe premature cardiac aging. Accumulating evidence indicates that autophagy plays an important role in obesity, cardiac aging and diseases. In this review, we will focus on the role of autophagy in obesity-related cardiac aging and diseases, and how it regulates age-dependent changes in the heart.

19.
Zhong Nan Da Xue Xue Bao Yi Xue Ban ; 44(4): 426-431, 2019 Apr 28.
Artigo em Chinês | MEDLINE | ID: mdl-31113919

RESUMO

OBJECTIVE: To examine the association between serum copper concentration and the prevalence of hypertension in patients with knee osteoarthritis (OA).
 Methods: A total of 935 patients who were aged ≥40 years and underwent routine checkups from October 2013 to November 2014 at the Health Management Center of Xiangya Hospital, Central South University were included. They were diagnosed as knee OA by weight-bearing bilateral anteroposterior radiography. Serum copper concentration was measured using the chemiluminescence method. Blood pressure was measured by an electronic sphygmomanometer. The association between serum copper concentration and hypertension was evaluated by conducting multivariable adjusted logistic regression.
 Results: Compared with the lowest quintile, the multivariable-adjusted odds ratio (OR) and related 95% confidence interval (95% CI) of hypertension were 1.46 (95% CI 1.02 to 2.09, P for trend=0.035) and 1.47 (95% CI 0.77 to 2.78, P for trend=0.032) in the total population and female subgroup of the highestest quintile, respectively. There was no significant association between serum copper and hypertension in male subgroup among OA patients (OR=1.21, 95% CI 0.76 to 1.93, P for trend=0.354).
 Conclusion: The serum copper concentration was significantly associated with the prevalence of hypertension in total population and female subgroup, but may not in male subgroup among patients with knee OA.


Assuntos
Hipertensão , Osteoartrite do Joelho , Adulto , Cobre , Estudos Transversais , Feminino , Humanos , Hipertensão/complicações , Masculino , Razão de Chances , Osteoartrite do Joelho/complicações , Fatores de Risco
20.
Talanta ; 200: 480-486, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31036212

RESUMO

A simpler, more rapid and selective biosensor has been developed for the sensitive detection of circulating miRNAs (c-miRNAs) based on duplex-specific nuclease (DSN)-assisted target recycling coupled with rolling circle amplification (RCA) using SYBR Gold Dye as a signal indicator. This biosensor exhibits a tremendously low detection limit of 0.3 fM of c-miRNAs and shows high selectivity for one-base mismatched c-miRNA. In addition, most importantly, the biosensor could accurately determine the amounts of target c-miRNA in total c-miRNAs isolated from human serums and human cancer cell lines, which confirms that the biosensor could be used to quantify c-miRNAs in complex biological samples and for the clinical early diagnosis of cancers based on c-miRNAs detection.


Assuntos
Técnicas Biossensoriais , MicroRNA Circulante/sangue , Endonucleases/química , Fluorescência , Técnicas de Amplificação de Ácido Nucleico , MicroRNA Circulante/isolamento & purificação , Corantes Fluorescentes/síntese química , Corantes Fluorescentes/química , Humanos , Células Tumorais Cultivadas
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