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1.
Sci Total Environ ; : 135482, 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31759700

RESUMO

This investigation demonstrated that aerobic granular sludge (AGS) could be cultivated rapidly in a single continuous-flowing membrane bioreactor (MBR) by introducing freely moved bio-carriers with a filling ratio of 10%. By operating the bioreactor for 28 days, AGS was successfully cultivated and kept stable for >2 months with a compact structure and clear shape, in which, extracellular polymeric substances played a key role in maintaining the stability of granular sludge structure. The microbial composition between AGS and attached biofilm was quite different, which indicated that the introduced bio-carriers improved the biodiversity within the bioreactor. Additionally, an explicit internal circulation was formed by the introduced bio-carriers, which was the main reason leading to the rapid formation of AGS. This is an interesting discovery and a novel approach to promote the rapid granulation of biomass in an MBR. Moreover, combining the biodegradation of AGS and filtration of membrane module, the bio-reactor achieved an excellent performance in removing CODCr (>90%) and TN (>85%) during the whole process.

2.
Orphanet J Rare Dis ; 14(1): 252, 2019 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-31722741

RESUMO

BACKGROUND: Animal studies suggested that blocking the activation of the mammalian target of rapamycin (mTOR) pathway might be effective to treat cardiac hypertrophy in LEOPARD syndrome (LS) caused by PTPN11 mutations. RESULTS: In the present study, mTOR pathway activity was examined in human myocardial samples from two patients with LS, four patients with hypertrophic cardiomyopathy (HCM), and four normal controls. The two patients with LS had p.Y279C and p.T468 M mutations of the PTPN11 gene, respectively. Although PTPN11 mutation showed initially positive regulation on phosphoinositide 3-kinase, overall the mTOR complex 1 pathway showed widely attenuated activity in LS. This included mildly hypophosphorylated mTOR and ribosomal protein S6 kinase and significantly hypophosphorylated Akt308 and ribosomal protein S6, which is similar to HCM. Akt473 is a basal molecule of the mTOR complex 2 pathway. Akt473 was less affected and showed hyperactivity in LS compared with HCM and normal controls. Additionally, MAPK/ERK kinase and ERK1/2 were significantly more phosphorylated in both HCM and LS than normal controls. CONCLUSIONS: In LS, the mTOR signaling pathway shows similar activity to HCM and is attenuated compared with normal controls. Thus, caution should be applied when using rapamycin to treat heart hypertrophy in LS.

3.
Am J Hypertens ; 2019 Oct 24.
Artigo em Inglês | MEDLINE | ID: mdl-31648306

RESUMO

BACKGROUND: Transient receptor potential melastatin subtype 8 (TRPM8) is a cold-sensing cation channel, mainly localized in the sensory neurons, which can be activated by menthol, a compound with a naturally cold sensation in mint. However, the effect of TRPM8 activation in inflammation and cardiac remodeling after myocardial infarction (MI) is not well defined. METHODS: TRPM8 knockout (KO) mice (TRPM8-/-) and their wild-type littermates, aged 8 weeks, were randomly divided into sham and MI groups and were fed with chow or chow plus menthol. RESULTS: Dietary menthol significantly attenuated MI injury, evidenced by decreased survival rates and plasma cardiac troponion I levels, reduced infarct size and cardiomyocytes, declined collagen deposition, and rescued cardiac function and hemodynamics. However, these effects of menthol disappeared when mice were lacking TRPM8. Furthermore, feeding of menthol ameliorated elevated expression of inflammatory cytokines and chemokines, and aggravated inflammation infiltration in the MI mice but not in TRPM8-/- mice. In addition, menthol treatment increased the release of calcitonin gene-related peptide (CGRP), which were absent in TRPM8-/- mice. CONCLUSIONS: In conclusion, our results suggest that dietary menthol can protect against inflammation and cardiac remodeling after MI through activation of TRPM8.

4.
Int J Occup Saf Ergon ; : 1-26, 2019 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-31661401

RESUMO

The construction industry is extremely high-risk, and safety checklist analysis (SCA) is a widely used approach for safety assessment. To overcome its limitations, this paper proposes an improved safety checklist analysis (ISCA) approach using intelligent video surveillance to replace on-site inspection. Then, a case study on metro tunnel construction is adopted to illustrate the process. First, the checklist is prepared. Second, the inspection items are correlated with construction areas, and intelligent cameras are positioned to cover the major areas of the construction site to guarantee that all the items can be checked. Thus, problems with inspection items are automatically identified and recorded. Third, the inspection items are marked by a remote scoring mechanism for safety assessment. Finally, the efficiency of the improved approach is tested by a comparative analysis among three groups. The application results indicate the feasibility of the improved approach for evaluating the safety management performance at construction sites.

5.
J Environ Manage ; 252: 109647, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31600685

RESUMO

This research built up a continuous dynamic flow filter membrane to treat diluted textile wastewater and basically investigated dynamic membrane fouling mechanism. By pre-depositing particles activated carbon (PAC) on membrane support material (MSM), a thin layer was formed on its surface, which showed excellent results in removing organic pollutants from diluted textile wastewater. Experimental data were regressed by the Langmuir, Freundlich, Temkin, Dubinin-Radushkevich (D-R) and Sips isotherm models. The three two-parameter isotherms (Temkin, D-R and Freundlich) were the models that best fitted, with respectively 0.977, 0.975 and 0.973 regression coefficients. D-R model has registered the maximum calculated adsorption capacity Qmd, cal. = 45.499 mg/g and the mean energy which was required to adsorb 1 mol of MB dye by the DM layer E = 4.249 kJ/mol; indicating the energy distribution onto heterogeneous surface of a physical adsorption process. Furthermore, kinetic models results showed that MB adsorption onto PAC at different initial concentrations follows the pseudo-second order. The obtained results also indicated that a flexible DM layer with different thickness can be formed from different amount of PAC pre-deposited on MSMs, which demonstrated that it was convenient to adjust the required DM thickness to filtrate a known initial concentration for >99% organic pollutants removal efficiency rate. However, DM fouling occurred on small pores MSMs; which resulted in an increase of the filtration pressure what have affected the filtration performance. PAC and MSMs surface morphology and texture structure, before and after filtration, were visualized respectively by Field Emission Scanning Electron Microscopy (FESEM), Fourier Transform Infra-Red and Attenuated Total Reflectance (FTIR/ATR). From these experimental results, a sustainable flux (>6.85 × 10-5 m/s) was established to discriminate no fouling from fouling conditions based on flux and TMP trends variance.


Assuntos
Poluentes Químicos da Água , Purificação da Água , Adsorção , Humanos , Cinética , Membranas Artificiais , Têxteis , Águas Residuárias
6.
Acta Biomater ; 100: 191-201, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31586729

RESUMO

Excessive inflammation and reduced angiogenesis are two major obstacles in burn wound healing and skin regeneration. Here we report the fabrication and application of a sophisticated hydrogel from chemically modified hyaluronic acid (HA), dextran (Dex), and ß-cyclodextrin (ß-CD) integrating resveratrol (Res) and vascular endothelial growth factor (VEGF) plasmid as the anti-inflammatory and pro-angiogenic components for burn wounds. Firstly, covalent alterations were conducted to obtain methacrylic acid anhydride modified HA (HAMA), N-hydroxyethylacrylamide (HEAA) modified Dex (Dex-HEAA), and poly(ethylene glycol) methyl acrylate (526) modified ß-CD (526-ß-CD), respectively. Secondly, anti-inflammatory substance Res was embedded into the lipophilic central cavity of 526-ß-CD to achieve a complex of 526-ß-CD-Res. Then hydrogels with different HAMA, Dex-HEAA, and 526-ß-CD-Res ratios were generated via UV irradiation. Lastly, plasmid DNA encoded with vascular endothelial growth factor (pDNA-VEGF) conjugating with polyethylenimine was loaded into the hydrogel scaffold. Combining the benefits of all components of the scaffold, the hydrogel embedded with Res and VEGF (Gel-Res/pDNA-VEGF) accelerated the splinted excisional burn wound healing, particularly by inhibiting inflammation response and promoting microvascular formation while being biocompatible. The Res and VEGF gene loaded hydrogel system can be considered as a promising wound dressing for the treatment of various types of wounds. STATEMENT OF SIGNIFICANCE: Combining the benefits of all components of the scaffold, the hydrogel embedded with Res and VEGF (Gel-Res/pDNA-VEGF) accelerated the splinted excisional burn wound healing, particularly by inhibiting inflammation response and promoting microvascular formation while being biocompatible. The Res and VEGF gene loaded hydrogel system can be considered as a promising wound dressing for the treatment of various types of wounds.

7.
Plant Cell ; 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31575723

RESUMO

Guanosine-3',5'-cyclic monophosphate (cGMP) is an important regulator in eukaryotes and cGMP-dependent protein kinase (PKG) plays a key role in perceiving cellular cGMP in diverse physiological processes in animals. However, the molecular identity, property, and function of PKG in plants remain elusive. This study has identified PKG from plants and characterized its role in mediating gibberellin (GA) response in rice. PKGs from plants are structurally unique with an additional type 2C protein phosphatase (PP2C) domain. Rice PKG possesses both protein kinase and phosphatase activities, and cGMP stimulates its kinase activity but inhibits its phosphatase activity. One of PKG's targets is GAMYB, a transcription factor in GA signaling, and the dual activities of PKG catalyze the reversible phosphorylation of GAMYB at Serine6, and modulate the nucleo-cytoplasmic distribution of GAMYB in response to GA. Loss of PKG impeded the nuclear-localization of GAMYB and abolished GAMYB function in GA response, leading to defects in GA-induced seed germination, internode elongation, and pollen viability. In addition to GAMYB, the results indicate that PKG has broader effects with multiple potential targets in particular involved in salt stress response.

8.
Heart Lung Circ ; 2019 Sep 19.
Artigo em Inglês | MEDLINE | ID: mdl-31635998

RESUMO

BACKGROUND: The prevalence of obstructive sleep apnoea (OSA) is high in patients with hypertrophic cardiomyopathy. The effect of septal myectomy on OSA is not clear. This study aimed to examine the association between hypertrophic obstructive cardiomyopathy and OSA before and after septal myectomy. METHOD: We included 85 consecutive patents with a confirmed diagnosis of hypertrophic obstructive cardiomyopathy who underwent septal myectomy. Polysomnography was performed in all patients before and 3 months after the surgery. RESULTS: Of the 85 patients, 49 (58%) were diagnosed with OSA. Patients with OSA were significantly older than those without OSA. The incidence of atrial fibrillation significantly increased during the perioperative period in patients with OSA (p = 0.03). The severity of OSA significantly increased 3 months after surgery, as determined by the apnoea-hypopnea index (AHI; p < 0.001), obstructive apnoea index (p = 0.024), and hypopnoea index (p = 0.003), whereas central apnoea index was decreased (p = 0.008). In the multivariate linear regression analysis, mean oxygen desaturation and time% with SpO2 <90% during sleep before surgery were significantly associated with increased AHI, independently of body mass index and sex (p = 0.026 and p = 0.007, respectively; adjusted R2 = 0.365). CONCLUSIONS: The severity of OSA significantly increased 3 months after septal myectomy as determined by AHI, obstructive apnoea index, and hypopnoea index. Mean oxygen saturation and time% with SpO2 <90% during sleep before surgery were independently associated with the increase of AHI. However, the specific mechanism of such deterioration of OSA after septal myectomy needs to be determined in detail.

9.
Artigo em Inglês | MEDLINE | ID: mdl-31588075

RESUMO

OBJECTIVES: This study aims to investigate the risk factors of in-hospital postoperative atrial fibrillation (POAF) and the impact of POAF on the clinical outcome in hypertrophic cardiomyopathy (HCM) patients who underwent myectomy. METHODS: Data from a total of 494 obstructive HCM patients, who had undergone preoperative cardiac magnetic resonance (CMR) testing and who underwent myectomy at Fuwai Hospital from June 2011 to June 2016, were collected. RESULTS: Multivariate logistic regression analysis showed that old age (odds ratio [OR], 4.326; 95% confidence interval [CI], 2.248-8.325; p <0.001), maximal left atrium volume (LAV) (OR, 1.137; 95% CI, 1.075-1.202; p <0.001), and hypertension (OR, 2.754; 95% CI, 1.262-6.007; p = 0.011) were associated with the incidence of POAF. In the patients without preoperative AF, Cox regression analysis demonstrated that POAF (p = 0.002), decreased left atrium (LA) ejection fraction (LAEF) (p = 0.036), concomitant procedure (p = 0.039), and postoperative residual moderate or severe mitral valve regurgitation (p = 0.030) were independent predictors of composite cardiovascular events. CONCLUSIONS: POAF indicated a poorer clinical outcome after myectomy for obstructive HCM patients, which was similar to those with preoperative AF. Elevated LAV was independently related to POAF onset in HCM patients who underwent myectomy.

10.
Plant Sci ; 288: 110218, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31521214

RESUMO

Salt stress is a major constraint to plant growth and development, and plants have developed sophisticated mechanisms to cope with it. AtAGO2, an argonaute protein, is known to play an important role in plant adaptation to salt stress; however, the molecular mechanism of this phenomenon remains essentially unexplored. Here, we performed the yeast two-hybrid assay and found an R3H-domain containing protein, designated as MUG13.4, interacting with AtAGO2. Further bimolecular fluorescence complement (BiFC), glutathione-S-transferase (GST) pull-down, and co-immunoprecipitation (Co-IP) assays confirmed that MUG13.4 interacted with AtAGO2, and MUG13.4 could affect the slicing activity of AtAGO2 associated with miR173. MUG13.4 and AtAGO2 were both predominantly expressed in seeds and roots. Phenotypic analyses of the single and double mutants under salt stress confirmed involvement of MUG13.4-AtAGO2 complex as a component of the salt tolerance mechanism. The mug13.4×ago2-1 double mutant displayed retarded growth and hypersensitivity to salt stress that was more pronounced than in mug13.4 or atago2-1 single mutants. TAS1c-tasiRNA generating system in Nicotiana benthamiana revealed that MUG13.4 could influence the slicing activity of AtAGO2. We also found that MUG13.4 increasingly changed the phenotype of slicer-defected mutants of AtAGO2 in response to salt stress. These findings suggested that the function of AtAGO2 upon salt stress was dependent on MUG13.4. Further investigation suggested that AtAGO2 improved Arabidopsis tolerance to salt stress by affecting operation of the SOS signaling cascade at the transcript level. Taken together, these findings reveal a novel function of MUG13.4 in adjusting Arabidopsis adaptation to salt stress.

11.
Stem Cell Res Ther ; 10(1): 154, 2019 Sep 11.
Artigo em Inglês | MEDLINE | ID: mdl-31506090

RESUMO

BACKGROUND: Autologous epidermal basal cell suspension therapy has been proven to be one of the most effective treatments for full-thickness wounds. However, we found there remain obvious defects that significantly confined the utilization and function of the epidermal basal cells (EBCs), especially the epidermal stem cells (ESCs) in it. This study investigated whether precoating fibronectin (FN) on the wound bed before spraying EBCs could overcome these defects and further explored its possible mechanisms. METHODS: In the in vitro study, EBCs were isolated from the donor skin of patients who needed skin grafting. Different concentrations of FN were used to precoat culture dishes before cell culture; the adherent efficiency, proliferation and migration ability of ESCs were analyzed and compared with traditional collagen IV precoating. In the in vivo study, Sprague-Dawley (SD) rats with full-thickness skin wounds were selected as full-thickness wounds' model. For the experiment groups, 20 µg/ml FN was precoated on the wound bed 10 min before EBC spray. The quality of wound healing was estimated by the residual wound area rate, wound healing time, and hematoxylin and eosin (H&E) staining. Expression of ESC markers, neovascular markers, inflammation markers, and collagen formation and degradation markers was elucidated by immunohistochemistry (IHC), immunofluorescence (IF), western blot (WB), and RT-qPCR analysis. RESULTS: The in vitro study showed that the dishes precoated with 20 µg/ml FN had a similar adherent efficiency and colony formation rate with collagen IV, but it could improve the proliferation and migration of ESCs significantly. Similarly, in the in vivo study, precoating FN on wound bed before EBC spray also significantly promote wound healing by improving ESCs' utilization efficiency, promoting angiogenesis, decreasing inflammations, and regulating collagen formation and degradation. CONCLUSION: FN precoating wound bed before EBC spray could significantly promote full-thickness wound healing by improving the utilization and function of the ESCs and further by promoting angiogenesis, decreasing inflammations, and regulating collagen formation and degradation.

12.
Angew Chem Int Ed Engl ; 58(45): 16134-16140, 2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31502363

RESUMO

Rubidium lead halides (RbPbX3 ), an important class of all-inorganic metal halide perovskites, are attracting increasing attention for photovoltaic applications. However, limited by its lower Goldschmidt tolerance factor t≈0.78, all-inorganic RbPbBr3 has not been reported. Now, the crystal structure, X-ray diffraction (XRD) pattern, and band structure of perovskite-phase RbPbBr3 has now been investigated. Perovskite-phase RbPbBr3 is unstable at room temperature and transforms to photoluminescence (PL)-inactive non-perovskite. The structural evolution and mechanism of the perovskite-non-perovskite phase transition were clarified in RbPbBr3 . Experimentally, perovskite-phase RbPbBr3 was realized through a dual-source chemical vapor deposition and annealing process. These perovskite-phase microspheres showed strong PL emission at about 464 nm. This new perovskite can serve as a gain medium and microcavity to achieve broadband (475-540 nm) single-mode lasing with a high Q of about 2100.

13.
Sci Total Environ ; 696: 133966, 2019 Dec 15.
Artigo em Inglês | MEDLINE | ID: mdl-31461693

RESUMO

Understanding the redistribution and retention of chromium(VI) (Cr(VI)) co-precipitated with silicate-containing ferrihydrite during aging is essential to the stabilization and immobilization of Cr(VI) in nature. In this work, Cr(VI) was removed by co-precipitated with silicate-containing ferrihydrite with various Si/Fe ratios at different precipitating pH. The co-precipitates were characterized by X-ray diffraction, transmission electron microscopy, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy before and after aging for 9 days at 80 °C. Results showed that silicate not only competed with Cr(VI) adsorbed on ferrihydrite surface by forming inner-sphere complexes, but also inhibited ferrihydrite transforming into more stable and compact iron phases. Ferrihydrite only transformed to hematite at pH0 5.0, and converted to hematite and goethite at pH0 7.5 and 10.0. Cr(VI) was initially removed by silicate-containing ferrihydrite with the removal efficiencies > 99.64% at initial pH of 5.0, and it was obviously incorporated into hematite and goethite during the transformation of ferrihydrite. The transformation products influenced the redistribution of Cr(VI), which was beneficial to the retention of Cr(VI) inside the co-precipitates, but not conducive to the adsorption for Cr(VI). The findings that Cr(VI) was removed by a common and metastable precursor of silicate-containing ferrihydrite can promote understanding of three-dimensional transfer and behavior of Cr(VI) during the transformation of silicate-containing ferrihydrite.

14.
Medicine (Baltimore) ; 98(33): e16905, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31415437

RESUMO

BACKGROUND: The prognosis of patients with coronary artery disease is mainly related to the extent of myocardium at risk. Proximal coronary arteries, especially the proximal left anterior descending coronary artery (LAD), supply a large part of the myocardium. In this analysis, we aimed to systematically compare the post percutaneous coronary interventional (PCI) outcomes observed with proximal vs non-proximal lesions of the left and right coronary arteries. METHODS: MEDLARS Online, Excerpta Medica database, www.ClinicalTrials.gov, and the Cochrane databases were searched for relevant studies comparing the post PCI outcomes reported on proximal vs non-proximal lesions of the coronary arteries. RevMan software version 5.3 was used to analyze the data to generate respective results. Odds ratios (OR) and 95% confidence intervals (CI) were derived to represent the results appropriately. RESULTS: Six studies with a total number of 11,109 participants who were enrolled between 1990 and 2015 were included in this analysis. The current results showed major adverse cardiac events (MACEs) (OR: 1.28, 95% CI: 1.14-1.45; P = .0001) and mortality (OR: 1.70, 95% CI: 1.43-2.03; P = .00001) to be significantly higher with proximal compared to non-proximal coronary lesions irrespective of the follow-up time periods. However, re-infarction (OR: 1.05, 95% CI: 0.80-1.38; P = .71), repeated revascularization (OR: 1.08, 95% CI: 0.92-1.27; P = .35) and stent thrombosis (OR: 0.59, 95% CI: 0.27-1.31; P = .20) were not significantly different.When patients specifically with LAD lesions were compared with associated non-proximal lesions, mortality was still significantly higher with proximal lesions (OR: 2.26, 95% CI: 1.52-3.36; P = .0001). However, when patients with right proximal coronary artery lesions were compared with the corresponding non-proximal lesions, no significant difference was observed in mortality. CONCLUSION: In-hospital and long-term MACEs and mortality were significantly higher in patients with proximal compared to non-proximal coronary lesions following PCI. In addition, mortality was significantly higher in patients with proximal LAD lesions whereas no significant difference was observed in patients with right proximal coronary artery lesions. Larger trials should further confirm these hypotheses.


Assuntos
Doença da Artéria Coronariana/cirurgia , Intervenção Coronária Percutânea/efeitos adversos , Vasos Coronários/patologia , Intervenção Coronária Percutânea/mortalidade , Complicações Pós-Operatórias/etiologia , Complicações Pós-Operatórias/mortalidade , Resultado do Tratamento
15.
Nat Prod Res ; : 1-9, 2019 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-31378086

RESUMO

Starting from indole-3-acetonitrile, a total of 66 new calycanthaceous alkaloid analogues were synthesised in excellent yields. The prepared compounds were evaluated for their biological activities against a broad range of plant pathogen fungi. The results of bioassays indicated that the majority of tested compounds displayed comparable or better in vitro bioactivities than the positive control. Notably, Compound a1 displayed a significant activities against B. cereus, Escherichia sp and R. solanacearum, even better than the positive control streptomycin and Penicillin, with the same MIC value of 15.63 µg mL-1. Compound a1 displayed a broad spectrum and remarkably activities among the tested calycanthaceous analogues and might be a novel potential leading compound for further development of antifungal agents. The results obtained in the study will be very helpful for further design and structural optimisation of calycanthaceous alkaloids as potential agrochemical lead for plant disease control.

16.
Analyst ; 144(18): 5497-5503, 2019 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-31386712

RESUMO

p-Nitrophenol (p-NP) pollutants are widely present in soil and aquatic environments and can seriously impair the health of living beings. Hence, a rapid, sensitive, and selective method for p-NP detection is urgently needed. Herein, for the first time, we successfully synthesized fluorescent carbon dots (CDs) from Bacillus cereus (BC) via a one-step hydrothermal process. The obtained CDs-BC can be applied as a rapid, highly selective, and sensitive sensor for p-NP detection. The fluorescence quenching efficiency of the CD-BC sensor exhibited excellent linear responses with p-NP concentrations at both 0.3-6.5 µM and 6.5-30 µM, with a detection limit of 0.11 µM. The mechanism of p-NP detection is based on the inner filter effect (IFE). Preliminary bacteria, cell, and animal studies showed that the as-prepared CDs-BC possess high photostability, excellent biocompatibility, low or no biotoxicity, and multicolor fluorescence emission properties; furthermore, they can be rapidly excreted from the body of mice, which suggests their potential for applications in the biomedical field.

17.
Diabetes Ther ; 2019 Jun 29.
Artigo em Inglês | MEDLINE | ID: mdl-31256352

RESUMO

INTRODUCTION: Type 2 diabetes mellitus (T2DM) is a major health issue, especially in patients with coexisting coronary artery disease (CAD). Patients with insulin-treated T2DM (ITDM) have worse outcomes than those with non-insulin-treated T2DM. Very few studies have compared short-term to long-term adverse cardiovascular outcomes following percutaneous coronary intervention (PCI) in patients on insulin therapy. Therefore, in this meta-analysis, we systematically compared short-term to long-term adverse cardiovascular outcomes in a population of patients with ITDM following PCI. METHODS: We searched for English-language publications focusing on PCI in patients with ITDM using specific search terms/phrases. All the participants accepted for inclusion in this meta-analysis were treated with a drug-eluting stent. Post-intervention adverse cardiovascular outcomes observed during short-term and long-term follow-up periods were assessed and compared. Statistical analysis was carried out using the popular RevMan 5.3 software. Odd ratios (OR) with 95% confidence intervals (CI) were calculated. RESULTS: Six studies comprising 1568 participants with ITDM in total were included in this simple meta-analysis. Patient enrollment periods varied but enrollment occurred during the years 1993-2012. When a fixed-effects statistical model was used, post-PCI adverse cardiovascular outcomes-such as major adverse cardiac events (MACEs) (OR 3.33, 95% CI 2.64-4.21; P = 0.00001), all-cause mortality (OR 5.73, 95% CI 3.37-9.73; P = 0.00001), myocardial infarction (MI) (OR 1.47, 95% CI 1.05-2.07; P = 0.02), and repeated revascularization (OR 4.78, 95% CI 3.29-6.94; P = 0.00001)-were found to be significantly more likely during the long-term follow-up period. A similar result was observed with a random-effects statistical model. CONCLUSION: Adverse cardiovascular outcomes post PCI were significantly more likely during the long-term follow-up period than during the short-term follow-up period in these patients with T2DM on insulin therapy. This hypothesis requires confirmation via new comparative trials that consider short-term and long-term follow-up periods.

18.
BMC Med Res Methodol ; 19(1): 93, 2019 05 08.
Artigo em Inglês | MEDLINE | ID: mdl-31068131

RESUMO

BACKGROUND: While it is widely acknowledged that family burden can be ameliorated with effective psycho-social interventions, how to measure family burden and define a valid cutoff to identify family caregivers in need of such interventions remains a key question. The purpose of the present study was to determine a statistically valid cutoff score for the Family Burden Interview Schedule (FBIS), using the cutoff scores of the Patient Health Questionnaire (PHQ-9) and the Generalized Anxiety Disorder Scale (GAD-7) as the reference. METHODS: The FBIS, PHQ-9, and GAD-7 were administered to a representative community sample of 327 family caregivers of schizophrenia patients. A FBIS cutoff score was determined using three different statistical methods: tree-based modeling, K-means clustering technique and linear regression. Contingency analysis was conducted to compare the FBIS cutoff with depression and anxiety scale scores. RESULTS: Findings proposed a cutoff score of 23 for the FBIS, with sensitivity being 76% for PHQ-9 and 74% for GAD-7, specificity being 68% for PHQ-9 and 67% for GAD-7. CONCLUSION: This cutoff score would enable health care providers to assess family caregivers at risk and provide necessary interventions to improve their quality of life.

19.
J Card Surg ; 34(7): 533-540, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31111576

RESUMO

BACKGROUND AND AIM: Recently alcohol septal ablation (ASA) has emerged as an alternative treatment for drug-refractory hypertrophic obstructive cardiomyopathy (HOCM) and a subgroup of HOCM patients with previous ASA may need myectomy. However, subsequent outcome and mechanism of residual obstruction has not been determined. This study aims to determine outcome after myectomy and mechanism of residual obstruction in HOCM patients with previous ASA. METHODS: From February 2009 to June 2017, 38 HOCM patients with previous ASA underwent surgical septal myectomy at our institution. Seventy-six patients who underwent surgical septal myectomy initially were included as the comparison group through one-to-two propensity score matching method. RESULTS: Fourteen available cardiac magnetic resonance images revealed inferior location and small area of infarcted myocardium induced by ASA in 12 patients and outside targeted location in two patients. During follow-up (median, 2.4; maximum, 7.8 years), event-free survival at 7 years was 83.2% in the previous ASA group and 94.6% in the comparison group, respectively (P = 0.0378). Multivariable analysis indicated previous ASA (hazard ratio, 4.28; 95% confidence intervals [CI], 1.20-15.26; P = 0.025) and postoperative left ventricular end-diastolic diameter (hazard ratio, 1.14; 95% CI, 1.05-1.23; P = 0.002) were independent predictors of adverse events. CONCLUSIONS: This study demonstrated that uncontrollable extent and location of infarcted myocardium induced by ASA may attribute to residual obstruction after previous ASA, and the long-term event-free survival after myectomy was inferior. It may provide special precaution to patient selection and the increased number of ASA practiced worldwide.

20.
Chem Commun (Camb) ; 55(32): 4691-4694, 2019 Apr 16.
Artigo em Inglês | MEDLINE | ID: mdl-30938736

RESUMO

An efficient Cu(ii)-catalyzed, C-H alkylation of BODIPY with a variety of alkyl diacyl peroxides has been developed for the first time, providing a late-stage and straightforward method for controllable synthesis of monoalkylated and dialkylated BODIPYs via a radical process that otherwise is difficult to obtain by literature methods. This chemo- and site-selective transformation will allow for the introduction of a variety of functionalities on the BODIPY core for highly versatile tethering to receptors and to other molecules of interest.


Assuntos
Compostos de Boro/química , Cobre/química , Corantes Fluorescentes/química , Peróxidos/química , Alquilação , Compostos de Boro/síntese química , Catálise , Corantes Fluorescentes/síntese química , Estrutura Molecular
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