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1.
Kidney Int ; 2022 May 02.
Artigo em Inglês | MEDLINE | ID: mdl-35513124

RESUMO

High-altitude polycythemia (HAPC) is a clinical syndrome that occurs in native inhabitants or long-term residents living at altitude. The kidney is one of the most affected organs. However, the clinical and kidney histopathological profiles of HAPC-related kidney disease have rarely been reported. Here, we report kidney biopsy-based clinicopathological study on this disease. HAPC was defined as excessive erythrocytosis [females, hemoglobin 190 g/L or more; males, 210 g/L or more] in patients living above an altitude of 2500 m for more than ten years. A total of 416 Tibetan patients underwent kidney biopsy between January 1, 2016, and November 31, 2020. Of these patients 17 met the diagnostic criteria for HAPC-related kidney disease. Clinically, these patients had a median urinary protein level of 2.5 g/24-hour (range 1.81-6.85). Twelve patients had hyperuricemia, nine had hypertension, and three had kidney insufficiency. On histopathology, glomerular hypertrophy, glomerular basement membrane thickening, podocyte foot process effacement, segmental glomerulosclerosis and global glomerulosclerosis were the main features. Extraglomerular arterial/arteriolar lesions were common, presenting as intimal fibrosis, hyalinosis and endothelial cell swelling/subintimal edema. Expansion of the arterial/arteriolar medial wall area characterized by smooth muscle cell proliferation was clearly observed, potentially indicating vascular remodeling. Hypoxia-inducible factor 2α was expressed in the kidney tissues of these patients. Thus, the pathological changes of HAPC-related kidney disease encompassed both glomerular and extraglomerular vascular lesions, suggesting a key role of both chronic hypoxia itself and secondary hemodynamic changes in the pathogenesis of this disease.

2.
Int J Biol Sci ; 18(6): 2392-2405, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35414778

RESUMO

G-protein-coupled receptors (GPCRs) signaling is critical to cell differentiation and activation. However, the function of GPCRs in osteoclast differentiation and activation remains unclear. We found that the G-protein coupled receptor 125 (GPCR 125) gene (Gpr125) gene was highly expressed in osteoclasts through RNA-sequencing technology, qRT-PCR, and Western blot analysis. We characterized the role of GPCR125 in osteoclast differentiation and activation by loss-of-function and gain-of-function methods in osteoclasts. Osteoclasts with lentivirus-mediated GPR125 silencing demonstrated a dramatic reduction in differentiation and impaired bone resorption function. In contrast, overexpression of Gpr125 in osteoclasts increased NFATC1 expression and enhanced osteoclast differentiation and enhanced osteoclast-mediated bone resorption. These results indicated that GPCR125 positively regulates osteoclast formation and function. Following receptor activator of nuclear factor kappa-Β ligand (RANKL) stimulation, the expression levels of MAPK signaling pathway proteins phosphorylated-ERK (p-ERK) and phosphorylated-p38 (p-p38) were significantly decreased in the Gpr125 knockdown (sh-GPR125) group compared to its control group. We also found that phosphorylated AKT (p-AKT) expression was downregulated, as well as nuclear factor kappa-B (NF-κB) signaling pathway protein phosphorylated-IKB alpha (p-IKBα). Our results demonstrated that GPCR125 positively regulates osteoclasts via RANKL-stimulated MAPK and AKT-NF-κB signaling pathways, and GPCR125 could potentially be utilized as a novel therapeutic target in bone related diseases including osteoporosis.


Assuntos
Reabsorção Óssea , Osteogênese , Animais , Reabsorção Óssea/metabolismo , Diferenciação Celular/genética , Camundongos , NF-kappa B/metabolismo , Osteoclastos/metabolismo , Osteogênese/genética , Proteínas Proto-Oncogênicas c-akt/metabolismo , Ligante RANK/metabolismo , Receptores Acoplados a Proteínas G/genética , Receptores Acoplados a Proteínas G/metabolismo , Transdução de Sinais/genética
3.
Environ Pollut ; 305: 119276, 2022 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-35405221

RESUMO

Widespread conversion of coastal wetlands into aquaculture ponds in coastal region often results in degradation of the wetland ecosystems, but its effects on sediment's potential to produce greenhouse gases remain unclear. Using field sampling, incubation experiments and molecular analysis, we studied the sediment CH4 production potential and the relevant microbial communities in a brackish marsh and the nearby aquaculture ponds in the Min River Estuary in southeastern China. Sediment CH4 production potential was higher in the summer and autumn months than in spring and winter months, and it was significantly correlated with sediment carbon content among all environmental variables. The mean sediment CH4 production potential in the aquaculture ponds (20.1 ng g-1 d-1) was significantly lower than that in the marsh (45.2 ng g-1 d-1). While Methanobacterium dominated in both habitats (41-59%), the overall composition of sediment methanogenic archaea communities differed significantly between the two habitats (p < 0.05) and methanogenic archaea alpha diversity was lower in the aquaculture ponds (p < 0.01). Network analysis revealed that interactions between sediment methanogenic archaea were much weaker in the ponds than in the marsh. Overall, these findings suggest that conversion of marsh land to aquaculture ponds significantly altered the sediment methanogenic archaea community structure and diversity and lowered the sediment's capacity to produce CH4.


Assuntos
Lagoas , Áreas Alagadas , Aquicultura , Archaea , Ecossistema , Metano
4.
Appl Opt ; 61(10): 2733-2742, 2022 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-35471345

RESUMO

Mass loss from wall surface bulge deformation can be used to estimate the strength loss of reinforcement, bond reduction, and ductility degradation, so it is very important to accurately measure the three-dimensional (3D) shape of on-site wall surface bulge. In this paper, we try to solve the problem by use of fringe projection profilometry. In the fringe projection patterns of wall surface bulge, the contrast of the fringes is very weak, and there are sometimes cracks in patterns. We first present a preprocessing method to inpaint fringes if there are damaged fringes caused by cracks. Then we propose a new, to the best of our knowledge, image decomposition model, total generalized variation (TGV)-Hilbert-block-matching (BM)3D, to effectively extract the fringe component. Finally, we use Fourier transform, phase unwrapping, and carrier-removal methods to obtain the unwrapped phase. We test the proposed method on a simulated fringe projection pattern and two real fringe projection patterns of wall surface bulge. We compare our method with the advanced total variation space-generalized functions space-BM3D, TV-Hilbert-L2, and Beppo-Levi-space-Hilbert-BM3D methods. In addition, we perform ablation experiments to prove that our preprocessing method is necessary. The experimental results demonstrate that our method can effectively measure the 3D shape of wall surface bulge from a single fringe projection pattern for the first time, to our knowledge.

5.
J Transl Med ; 20(1): 108, 2022 03 04.
Artigo em Inglês | MEDLINE | ID: mdl-35246141

RESUMO

BACKGROUND: Hypertension is highly prevalent and associated with the elevated risks of cardiovascular diseases, dementia, and physical disabilities among adults. Although the correlation between bilirubin and hypertension has been reported, the observation in quinquagenarian population is scarce. We aimed to examine bilirubin-hypertension association in Guankou Ageing Cohort Study. METHODS: Participants ≥ 55 years were recruited and their questionnaires and physical examination data were collected. Kaplan-Meier survival analysis and Cox proportional hazards regression were implemented to assess the hypertension risk. The non-liner dose-response relationships of bilirubin-hypertension were determined by restricted cubic spline (RCS) models. Receiver operating characteristic (ROC) curves and multiple factors analysis (MFA) were performed to evaluate the predictive abilities. RESULTS: 1881 eligible participants (male 43.75%, female 56.25%) with the median age of 61.00 (59.00-66.00) were included. The hazard ratio (HR, 95% CI) of serum total bilirubin (STB) and unconjugated bilirubin (UCB) were 1.03 (1.01-1.05) and 1.05 (1.03-1.07), while conjugated bilirubin (CB) showed a weak protective effect with the HR of 0.96 (0.92-0.99), and the associations remained significant in all models. RCS analyses further indicated the similar bidirectional effects of STB and UCB with the cut-off of 12.17 µmol/L and 8.59 µmol/L, while CB exhibited inverse bidirectional dose-response relationship with a cut-off of 3.47 µmol/L. ROC curves and MFA showed baseline STB combined with age, BMI, and waist circumference could well discriminate the low and high of hypertension risk. CONCLUSIONS: Our findings suggested the higher levels of total and unconjugated bilirubin were hazardous factors of hypertension, while an inverse effect presented when more bilirubin was conjugated.


Assuntos
Bilirrubina , Hipertensão , Adulto , Envelhecimento , China/epidemiologia , Estudos de Coortes , Feminino , Seguimentos , Humanos , Masculino , Estudos Prospectivos , Fatores de Risco
6.
Front Genet ; 13: 825974, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35154285

RESUMO

Animal olfactory systems evolved with changes in habitat to detect odor cues from the environment. The aquatic environment, as a unique habitat, poses a formidable challenge for olfactory perception in animals, since the higher density and viscosity of water. The olfactory system in snakes is highly specialized, thus providing the opportunity to explore the adaptive evolution of such systems to unique habitats. To date, however, few studies have explored the changes in gene expression features in the olfactory systems of aquatic snakes. In this study, we carried out RNA sequencing of 26 olfactory tissue samples (vomeronasal organ and olfactory bulb) from two aquatic and two non-aquatic snake species to explore gene expression changes under the aquatic environment. Weighted gene co-expression network analysis showed significant differences in gene expression profiles between aquatic and non-aquatic habitats. The main olfactory systems of the aquatic and non-aquatic snakes were regulated by different genes. Among these genes, RELN may contribute to exploring gene expression changes under the aquatic environment by regulating the formation of inhibitory neurons in the granular cell layer and increasing the separation of neuronal patterns to correctly identify complex chemical information. The high expression of TRPC2 and V2R family genes in the accessory olfactory systems of aquatic snakes should enhance their ability to bind water-soluble odor molecules, and thus obtain more information in hydrophytic habitats. This work provides an important foundation for exploring the olfactory adaptation of snakes in special habitats.

7.
Adv Sci (Weinh) ; 9(12): e2105742, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-35187853

RESUMO

Repeatability and high adhesion toughness are usually contradictory for common polymer adhesives. Repeatability requires temporary interactions between the adhesive and the substrate, while high adhesion toughness is usually achieved by permanent bonding. Integrating these two features into one adhesive system is still a daunting challenge. Here, the development of a series of viscoelastic elastomers composed of a soft and hard segment is reported, which exhibit tough, instant, yet repeatable adhesion to a variety of soft and hard surfaces. Such a combination of mutually exclusive properties is attributed to the synergy of high mobility of polymer chains and massive viscoelastic dissipation of the elastomers around the interface. By optimizing the relaxation time and mechanical dissipation, the resulting adhesives can achieve a tough yet repeatable adhesion toughness above 2000 J m-2 , superior to the best-in-class commercial adhesives. Numerous acrylate monomers are proven applicable to the preparation of such adhesives, validating the universality of the fabrication method. The application of these elastomers as adhesive and protective layers in soft electronics by virtue of their universal and tough adhesion to various soft and hard substrates is also demonstrated.


Assuntos
Adesivos , Elastômeros , Adesivos/química , Elastômeros/química , Fenômenos Físicos , Polímeros/química
8.
Int J Gen Med ; 15: 1225-1238, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35173463

RESUMO

PURPOSE: Although several studies have explored the association of sex hormones with glucose metabolism, the association between sex hormones and body fat distribution, which is closely related to insulin resistance, has not been fully elucidated. We have tried to explore the relationship of testosterone (T) and estradiol (E2) with visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) mass in Chinese men with different obese and metabolic statuses. PATIENTS AND METHODS: A total of 128 men from the Health Management Center of the Second Xiangya Hospital, Central South University were collected and grouped in accordance with their obese and metabolic syndrome (MS) statuses: metabolically healthy non-overweight/obese men (MHNO), metabolically healthy overweight/obese men (MHO) and metabolically unhealthy overweight/obese men (MUO). Multiple regression analyses were performed to estimate contributions of sex hormones levels to the variations of body fat distribution and the contributions of body fat distribution to the variations of sex hormone levels. RESULTS: With fat mass parameters as independent variables, SAT had a strong negative association with T in MHNO (ß = -2.772, P = 0.034), VAT was positively correlated with E2 in MHO (ß = 22.269, P = 0.009), and SAT was negatively associated with T in MUO (ß = -3.315, P = 0.010). With sex hormones as independent variables, E2 positively correlated with VAT (ß = -176.259, P = 0.048), while T negatively correlated with VAT in MHO (ß = 183.150, P = 0.029). In MUO, an inverse association of T with SAT was observed (ß = -213.689, P = 0.021). CONCLUSION: E2 and VAT had a mutual influence, thus resulting in a vicious circle, and the negative correlation between T and VAT may be related to the decrease of the MS occurrence in the MHO group. There were bi-directional relationships between sex hormones and fat distribution in men with different obese and metabolic statuses. TRIAL REGISTRATION: Chinese Clinical Trial Registry, ChiCTR-EOC-16010194. Retrospectively registered.

9.
J Opt Soc Am A Opt Image Sci Vis ; 39(2): 239-249, 2022 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-35200960

RESUMO

Simultaneous speckle reduction and contrast enhancement for electronic speckle pattern interferometry (ESPI) fringe patterns is a challenging task. In this paper, we propose a joint enhancement and denoising method based on the oriented variational Retinex model for ESPI fringe patterns with low contrast or uneven illumination. In our model, we use the structure prior to constrain the illumination and introduce a fractional-order differential to constrain the reflectance for enhancement, then use the second-order partial derivative of the reflectance as the denoising term to reduce noise. The proposed model is solved using the sequential method to obtain piecewise smoothed illumination and noise-suppressed reflectance sequentially, which avoids remaining noise in the illumination and reflectance map. After obtaining the refined illuminance and reflectance, we substitute the gamma-corrected illuminance into the camera response function to further adjust the reflectance as the final enhancement result. We test our proposed method on two non-uniform illumination computer-simulated and two low-contrast experimentally obtained ESPI fringe patterns. Finally, we compare our method with three other joint enhancement and denoising variational Retinex methods.

10.
J Agric Food Chem ; 70(9): 2851-2863, 2022 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-35226498

RESUMO

Based on the structural characteristics of the cryptolepine alkaloid, a series of new quindoline derivatives bearing various substituents were prepared and evaluated for their fungicidal and antibacterial activities. Bioassay results showed that compound D7 displayed superior in vitro fungicidal activities against Sclerotinia sclerotiorum, Botrytis cinerea, Fusarium graminearum, and Rhizoctonia solani with EC50 values of 0.780, 3.62, 1.59, and 2.85 µg/mL, respectively. Compound A7 showed apparent antibacterial activities toward Xanthomonas oryzae pv. oryzae with a minimum inhibitory concentration (MIC) value of 3.12 µg/mL. Significantly, in vivo antifungal activity suggested that the curative effect (98.3%) of compound D7 was comparable to that of the positive control azoxystrobin (96.7%) at 100 µg/mL. Preliminary mechanistic studies showed that compound D7 might cause mycelial abnormality of S. sclerotiorum, cell membrane breakage, accumulation of reactive oxygen species (ROS), and inhibition of sclerotia formation. Therefore, compound D7 could be a novel broad-spectrum fungicidal candidate against plant fungal diseases.


Assuntos
Fungicidas Industriais , Alcaloides Indólicos , Alcaloides , Antifúngicos/química , Fungicidas Industriais/química , Alcaloides Indólicos/química , Alcaloides Indólicos/farmacologia , Indóis , Estrutura Molecular , Quinolinas , Relação Estrutura-Atividade
11.
Brief Bioinform ; 23(2)2022 03 10.
Artigo em Inglês | MEDLINE | ID: mdl-35043159

RESUMO

Although drug combinations in cancer treatment appear to be a promising therapeutic strategy with respect to monotherapy, it is arduous to discover new synergistic drug combinations due to the combinatorial explosion. Deep learning technology holds immense promise for better prediction of in vitro synergistic drug combinations for certain cell lines. In methods applying such technology, omics data are widely adopted to construct cell line features. However, biological network data are rarely considered yet, which is worthy of in-depth study. In this study, we propose a novel deep learning method, termed PRODeepSyn, for predicting anticancer synergistic drug combinations. By leveraging the Graph Convolutional Network, PRODeepSyn integrates the protein-protein interaction (PPI) network with omics data to construct low-dimensional dense embeddings for cell lines. PRODeepSyn then builds a deep neural network with the Batch Normalization mechanism to predict synergy scores using the cell line embeddings and drug features. PRODeepSyn achieves the lowest root mean square error of 15.08 and the highest Pearson correlation coefficient of 0.75, outperforming two deep learning methods and four machine learning methods. On the classification task, PRODeepSyn achieves an area under the receiver operator characteristics curve of 0.90, an area under the precision-recall curve of 0.63 and a Cohen's Kappa of 0.53. In the ablation study, we find that using the multi-omics data and the integrated PPI network's information both can improve the prediction results. Additionally, the case study demonstrates the consistency between PRODeepSyn and previous studies.


Assuntos
Redes Neurais de Computação , Mapas de Interação de Proteínas , Linhagem Celular , Combinação de Medicamentos , Aprendizado de Máquina
12.
Environ Technol ; 43(12): 1819-1832, 2022 May.
Artigo em Inglês | MEDLINE | ID: mdl-33206008

RESUMO

Pyrolysis can realise the harmlessness, reduction and resource utilisation of petroleum sludge in a short period. In the present work, a tank bottom sludge (SSOS) and a landing sludge (SLOS) from Shengli Oilfield were used for experimental research. Thermogravimetric testing is used to initially determine the optimal range of pyrolysis temperature. Pyrolysis experiments were performed in a tube furnace reactor. Pyrolysis products were collected and analysed separately. The char yield of SSOS and SLOS were 50% and 70%, respectively. Although there are differences in the oil content of the two types of petroleum sludge, the oil yield remained nearly the same, which were both between 7% and 8%. As the pyrolysis temperature was raised to 500°C, the yield of each product did not change greatly while their composition had obvious changes. High temperature is more conducive to the production of small molecule products. Result showed that pyrolysis treatment of petroleum sludge can effectively recover energy materials in the form of pyrolysis gas and oil. The heating value of char is lower than that of petroleum sludge, which means that char is not suitable for direct use as fuel. Pyrolysis treatment also showed good curing effect on Cr, which reached 85%. However, the solidification effect decreased as pyrolysis temperature increasing. It is necessary to pay attention to the heavy metal contained in char as soil improver. The rich surface structure of char provides evidence to produce high value-added carbon materials.


Assuntos
Metais Pesados , Petróleo , Temperatura Alta , Pirólise , Esgotos/química
13.
Nephrology (Carlton) ; 27(2): 155-161, 2022 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-34713949

RESUMO

AIM: Hydroxychloroquine (HCQ) is used to control proteinuria in IgA Nephropathy (IgAN) However, its efficacy and safety in pregnant IgAN patients remains unknown. This study aimed to verify the safety of HCQ in pregnant IgAN patients and compare renal function and pregnancy outcomes with those of patients not treated with HCQ. METHODS: We retrospectively reviewed medical records of all pregnant IgAN patients and singleton gestations at Peking University First Hospital from 2003-2021. Patients who did and did not receive HCQ treatment during pregnancy were compared. RESULTS: We found no significant pre- or post-pregnancy differences in proteinuria or renal function between the two groups. However, the HCQ (+) group had higher proteinuria at the time of kidney biopsy (2.04 [1.26, 2.56] g/d vs. 0.80 [0.44, 1.11] g/d, P < .001); the proteinuria level at HCQ therapy initiation was also higher than that at the beginning of pregnancy (1.87 [1.30, 2.59] g/d vs. 1.08 [0.75, 1.50] g/d, P = .001). Despite no difference in preterm birth, birth weight, preeclampsia or postpartum haemorrhage, the proportion of patients with a previous history of spontaneous abortion was higher in the HCQ (+) group than in the HCQ (-) group (48.0% vs. 20.6%, P = .010). The eGFR (regression coefficient, 0.981; 95%CI 0.964-0.998) was a predictive factor for obstetrical complications. CONCLUSION: HCQ is safe for IgAN treatment during pregnancy with effective reduction of proteinuria. HCQ might also be helpful in patients with a history of spontaneous abortion.


Assuntos
Glomerulonefrite por IGA/tratamento farmacológico , Hidroxicloroquina/uso terapêutico , Complicações na Gravidez/tratamento farmacológico , Adulto , Estudos de Coortes , Feminino , Glomerulonefrite por IGA/fisiopatologia , Humanos , Hidroxicloroquina/efeitos adversos , Testes de Função Renal , Gravidez , Resultado da Gravidez , Estudos Retrospectivos , Resultado do Tratamento
14.
J Nephrol ; 35(2): 429-440, 2022 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33591553

RESUMO

BACKGROUND: Hydroxychloroquine (HCQ) has been used as a supportive therapy for IgA nephropathy (IgAN). We aimed to determine the long-term efficacy and safety of HCQ therapy in patients with IgAN. METHODS: A total of 180 patients with IgAN who had received HCQ therapy for at least 1 year were enrolled in this study. The changes in proteinuria and the estimated glomerular filtration rate (eGFR) were analyzed during the follow-up period. RESULTS: The level of proteinuria decreased from 1.69 [1.24, 2.30] to 1.01 [0.59, 1.74] g/day (- 37.58 [- 57.52, 8.24] %, P < 0.001) at 12 months and to 1.00 [0.59, 1.60] g/day (- 55.30 [- 71.09, - 3.44] %, P < 0.001) at 24 months. There was no significant change in the eGFR of these patients at 12 months (65.82 ± 25.22 vs. 63.93 ± 25.96 ml/min/1.73 m2, P = 0.411); however, the eGFR decreased from 65.82 ± 25.22 to 62.15 ± 25.81 ml/min/1.73 m2 at 24 months (P = 0.003). The cumulative frequency of all patients with a 50% decrease in proteinuria was 72.78% at 12 months. Sixty (33.3%) patients changed to corticosteroid therapy during the follow-up period. No serious adverse effects were documented during HCQ treatment. CONCLUSIONS: HCQ effectively and safely reduces proteinuria in IgAN patients with different levels of eGFR, supporting the maintenance of stable kidney function in the long term.


Assuntos
Glomerulonefrite por IGA , Hidroxicloroquina , Taxa de Filtração Glomerular , Glomerulonefrite por IGA/complicações , Glomerulonefrite por IGA/diagnóstico , Glomerulonefrite por IGA/tratamento farmacológico , Humanos , Hidroxicloroquina/efeitos adversos , Proteinúria/induzido quimicamente , Proteinúria/etiologia
15.
J Med Virol ; 94(5): 1967-1975, 2022 05.
Artigo em Inglês | MEDLINE | ID: mdl-34967028

RESUMO

We aimed to assess whether blood glucose control can be used as predictors for the severity of 2019 coronavirus disease (COVID-19) and to improve the management of diabetic patients with COVID-19. A two-center cohort with a total of 241 confirmed cases of COVID-19 with definite outcomes was studied. After the diagnosis of COVID-19, the clinical data and laboratory results were collected, the fasting blood glucose levels were followed up at initial, middle stage of admission and discharge, the severity of the COVID-19 was assessed at any time from admission to discharge. Hyperglycemia patients with COVID-19 were divided into three groups: good blood glucose control, fair blood glucose control, and blood glucose deterioration. The relationship of blood glucose levels, blood glucose control status, and severe COVID-19 were analyzed by univariate and multivariable regression analysis. In our cohort, 21.16% were severe cases and 78.84% were nonsevere cases. Admission hyperglycemia (adjusted odds ratio [aOR], 1.938; 95% confidence interval [95% CI], 1.387-2.707), mid-term hyperglycemia (aOR, 1.758; 95% CI, 1.325-2.332), and blood glucose deterioration (aOR, 22.783; 95% CI, 2.661-195.071) were identified as the risk factors of severe COVID-19. Receiver operating characteristic (ROC) curve analysis, reaching an area under ROC curve of 0.806, and a sensitivity and specificity of 80.40% and 68.40%, respectively, revealed that hyperglycemia on admission and blood glucose deterioration of diabetic patients are potential predictive factors for severe COVID-19. Our results indicated that admission hyperglycemia and blood glucose deterioration were positively correlated with the risk factor for severe COVID-19, and deterioration of blood glucose may be more likely to the occurrence of severe illness in COVID-19.


Assuntos
COVID-19 , Diabetes Mellitus , Hiperglicemia , Glicemia/análise , COVID-19/complicações , COVID-19/epidemiologia , Estudos de Coortes , Diabetes Mellitus/epidemiologia , Humanos , Hiperglicemia/epidemiologia , Prognóstico , Estudos Retrospectivos , Fatores de Risco
16.
Eur J Med Chem ; 227: 113937, 2022 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-34710744

RESUMO

Evodiamine and rutaecarpine are two alkaloids isolated from traditional Chinese herbal medicine Evodia rutaecarpa, which have been reported to have various biological activities in past decades. To explore the potential applications for evodiamine and rutaecarpine alkaloids and their derivatives, various kinds of evodiamine and rutaecarpine derivatives were designed and synthesized. Their antifungal profile against six phytopathogenic fungi Rhizoctonia solani, Botrytis cinerea, Fusarium graminearum, Fusarium oxysporum, Sclerotinia sclerotiorum, and Magnaporthe oryzae were evaluated for the first time. Furthermore, a series of modified imidazole derivatives of rutaecarpine were synthesized to investigate the structure-activity relationship. The results of antifungal activities in vitro showed that imidazole derivative of rutaecarpine A1 exhibited broad-spectrum inhibitory activities against R. solani, B. cinerea, F. oxysporum, S. sclerotiorum, M. oryzae and F. graminearum with EC50 values of 1.97, 5.97, 12.72, 2.87 and 16.58 µg/mL, respectively. Preliminary mechanistic studies showed that compound A1 might cause mycelial abnormalities of S. sclerotiorum, mitochondrial distortion and swelling, and inhibition of sclerotia formation and germination. Moreover, the curative effects of compound A1 were 94.7%, 81.5%, 80.8%, 65.0% at 400, 200, 100, 50 µg/mL in vivo experiments, which was far more effective than the positive control azoxystrobin. Significantly, no phytotoxicity of compound A1 on oilseed rape leaves was observed obviously even at a high concentration of 400 µg/mL. Therefore, compound A1 is expected to be a novel leading structure for the development of new antifungal agents.


Assuntos
Antifúngicos/farmacologia , Desenho de Fármacos , Alcaloides Indólicos/farmacologia , Quinazolinas/farmacologia , Antifúngicos/síntese química , Antifúngicos/química , Ascomicetos/efeitos dos fármacos , Botrytis/efeitos dos fármacos , Relação Dose-Resposta a Droga , Fusarium/efeitos dos fármacos , Alcaloides Indólicos/síntese química , Alcaloides Indólicos/química , Testes de Sensibilidade Microbiana , Estrutura Molecular , Quinazolinas/síntese química , Quinazolinas/química , Rhizoctonia/efeitos dos fármacos , Relação Estrutura-Atividade
17.
Genome Biol ; 23(1): 20, 2022 01 12.
Artigo em Inglês | MEDLINE | ID: mdl-35022082

RESUMO

Here, we present a multi-modal deep generative model, the single-cell Multi-View Profiler (scMVP), which is designed for handling sequencing data that simultaneously measure gene expression and chromatin accessibility in the same cell, including SNARE-seq, sci-CAR, Paired-seq, SHARE-seq, and Multiome from 10X Genomics. scMVP generates common latent representations for dimensionality reduction, cell clustering, and developmental trajectory inference and generates separate imputations for differential analysis and cis-regulatory element identification. scMVP can help mitigate data sparsity issues with imputation and accurately identify cell groups for different joint profiling techniques with common latent embedding, and we demonstrate its advantages on several realistic datasets.


Assuntos
Sequenciamento de Cromatina por Imunoprecipitação , Cromatina , Análise por Conglomerados , RNA-Seq , Sequências Reguladoras de Ácido Nucleico , Análise de Célula Única/métodos
18.
Front Microbiol ; 12: 753688, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34956118

RESUMO

Although the presence of live microbes in utero remains under debate, newborn gastrointestinal bacteria are undoubtedly important to infant health. Measuring bacteria in meconium is an ideal strategy to understand this issue; however, the low efficiency of bacterial DNA extraction from meconium has limited its utilization. This study aims to improve the efficiency of bacterial DNA extraction from meconium, which generally has low levels of microflora but high levels of PCR inhibitors in the viscous matrix. The research was approved by the ethical committee of the Xiamen Maternity and Child Health Care Hospital, Xiamen, China. All the mothers delivered naturally, and their newborns were healthy. Meconium samples passed by the newborns within 24 h were collected. Each sample was scraped off of a sterile diaper, transferred to a 5-ml sterile tube, and stored at -80°C. For the assay, a freeze-thawing sample preparation protocol was designed, in which a meconium-InhibitEX buffer mixture was intentionally frozen 1-3 times at -20°C, -80°C, and (or) in liquid nitrogen. Then, DNA was extracted using a commercial kit and sequenced by 16S rDNA to verify the enhanced bacterial DNA extraction efficiency. Ultimately, we observed the following: (1) About 30 mg lyophilized meconium was the optimal amount for DNA extraction. (2) Freezing treatment for 6 h improved DNA extraction at -20°C. (3) DNA extraction efficiency was significantly higher with the immediate thaw strategy than with gradient thawing at -20°C, -80°C, and in liquid nitrogen. (4) Among the conditions of -20°C, -80°C, and liquid nitrogen, -20°C was the best freezing condition for both improving DNA extraction efficiency and preserving microbial species diversity in meconium, while liquid nitrogen was the worst condition. (5) Three freeze-thaw cycles could markedly enhance DNA extraction efficiency and preserve the species diversity of meconium microflora. We developed a feasible freeze-thaw pretreatment protocol to improve the extraction of microbial DNA from meconium, which may be beneficial for newborn bacterial colonization studies.

19.
J Agric Food Chem ; 69(48): 14467-14477, 2021 Dec 08.
Artigo em Inglês | MEDLINE | ID: mdl-34843231

RESUMO

Crop diseases caused by fungi threaten food security and exacerbate the food crisis. Inspired by the application of fungicide candidates from natural products in agrochemical discovery, a series of luotonin A derivatives were designed, synthesized, and evaluated for their antifungal activities against five plant fungi. Most of these compounds exhibited significant fungicidal activity against Botrytis cinerea in vitro with EC50 values less than 1 µg/mL. Among them, compounds w7, w8, w12, and w15 showed superior antifungal activity against B. cinerea with EC50 values of 0.036, 0.050, 0.042, and 0.048 µg/mL, respectively, which were more potent than boscalid (EC50 = 1.790 µg/mL). Preliminary mechanism studies revealed that compound w7 might pursue its antifungal activity by disrupting the fungal cell membrane and cell wall. Moreover, in vivo bioassay also indicated that compound w7 could be effective for the control of B. cinerea. The above results evidenced the potential of luotonin A derivatives as novel and promising candidate fungicides.


Assuntos
Antifúngicos , Fungicidas Industriais , Antifúngicos/farmacologia , Botrytis , Fungos , Fungicidas Industriais/farmacologia , Pirróis , Quinonas , Relação Estrutura-Atividade
20.
Appl Opt ; 60(33): 10322-10331, 2021 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-34807040

RESUMO

In practical measurement, we often need to measure the shape of objects with patterns or letters. As far as we know, no paper has ever reported the shape measurement for objects with patterns or letters by Fourier fringe projection profilometry (FPP). In this paper, we propose a method based on the variational decomposition TV-Hilbert-L2 model and multi-scale Retinex (MSR) to measure the shape of objects with patterns and letters by Fourier FPP. In this method, we first use the TV-Hilbert-L2 model to obtain the fringe part, then perform MSR enhancement on the fringe part, and finally decompose the enhanced fringe part with TV-Hilbert-L2 again. We evaluate the performance of this method via application to one computer-simulated noisy fringe projection pattern and two experimental fringe projection patterns with different types of patterns or letters, and comparison with the Fourier transform method, the variational image decomposition TV-Hilbert-L2 model. Furthermore, we apply the proposed method to the dynamic three-dimensional shape measurement of hand posture with pattern. The experimental results show that our method can effectively measure the dynamic shape of objects with patterns or letters from a single-frame fringe projection pattern.

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