Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 481
Filtrar
1.
Chemosphere ; 262: 128366, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33182086

RESUMO

The embedding microbial fuel cell (MFC) into constructed wetlands (CW) to form CW-MFC bears the potential to obtain bioelectricity and a clean environment. In this study, a bibliometric analysis using VOSviewer based on Web of Science data was conducted to provide an overview by tracing the development footprint of this technology. The countries, institutions, authors, key terms, and keywords were tracked and corresponding mapping was generated. From 2012 to September 2020, 442 authors from 129 organizations in 26 countries published 135 publications in 42 journals with total citation of 3139 times were found. The key terms analysis showed four clusters: bioelectricity generation performance, mechanism study, refractory pollutants removal, and enhanced conventional contaminants removal. Further research themes include exploring the biochemical properties of electrochemically active bacteria, emerging contaminants removal, effective bioelectricity harvest and the use, and biosensor development as well as scaling-up for real field application. The bibliometric results provide valuable references and information on potential research directions for future studies.


Assuntos
Fontes de Energia Bioelétrica , Eliminação de Resíduos Líquidos , Áreas Alagadas , Bactérias , Bibliometria , Eletricidade , Eletrodos , Águas Residuárias
2.
World J Gastroenterol ; 26(39): 5959-5969, 2020 Oct 21.
Artigo em Inglês | MEDLINE | ID: mdl-33132647

RESUMO

Due to the rapid progression and poor prognosis of esophageal cancer (EC), the early detection and diagnosis of early EC are of great value for the prognosis improvement of patients. However, the endoscopic detection of early EC, especially Barrett's dysplasia or squamous epithelial dysplasia, is difficult. Therefore, the requirement for more efficient methods of detection and characterization of early EC has led to intensive research in the field of artificial intelligence (AI). Deep learning (DL) has brought about breakthroughs in processing images, videos, and other aspects, whereas convolutional neural networks (CNNs) have shone lights on detection of endoscopic images and videos. Many studies on CNNs in endoscopic analysis of early EC demonstrate excellent performance including sensitivity and specificity and progress gradually from in vitro image analysis for classification to real-time detection of early esophageal neoplasia. When AI technique comes to the pathological diagnosis, borderline lesions that are difficult to determine may become easier than before. In gene diagnosis, due to the lack of tissue specificity of gene diagnostic markers, they can only be used as supplementary measures at present. In predicting the risk of cancer, there is still a lack of prospective clinical research to confirm the accuracy of the risk stratification model.

3.
ChemSusChem ; 2020 Nov 16.
Artigo em Inglês | MEDLINE | ID: mdl-33200545

RESUMO

Converting CO2 to high value-added C2 hydrocarbons by CO2 reduction reaction attracted attention due to higher energy density, readiness for transportation, and established utilization infrastructure. Herein, it was demonstrated that tailoring the copper catalyst morphology by forming nanopyramids offers alternative routes to promote C2 production. Using density functional theory calculations, five polycrystalline Cu nanopyramids with various orientations, shapes, and exposing facets were investigated. Three investigated nanopyramids favored the C2 production to different extents due to anomalous C-C coupling behaviors. The underlying reason for such C-C coupling was the pyramidal effect on under-coordinated Cu (111) surface from the nanopyramids. The pyramidal effect includes improved *CO adsorption, geometrically preferable sites for C-C coupling, and enhanced electron transfer. Based on these results, a C2 active site screening principle was developed: an extended "square" principle, which can serve as a new morphology design rule for efficient catalyst development.

4.
Nanoscale Horiz ; 2020 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-33237052

RESUMO

Two-dimensional (2D) magnets simultaneously possessing a high transition temperature and large perpendicular magnetic anisotropy are extremely rare, but essential for highly efficient spintronic applications. By using ab initio and global minimization approaches, we for the first time report a completely planar hypercoordinate metalloborophene (α-FeB3) with high stability, unusual stoichiometry and exceptional magnetoelectronic properties. The α-FeB3 monolayer exhibits room-temperature ferromagnetism (Tc = 480 K), whose origin is first revealed by the B-mediated RKKY interaction in the 2D regime. Its perpendicular magnetic anisotropy is almost six times larger than that of the experimentally realized 2D CrI3 and Fe3GeTe2. Moreover, metallic α-FeB3 shows n- and p-type Dirac transport with a high Fermi velocity in both spin channels. Our results not only highlight a promising 2D ferromagnet for advanced spintronics, but also pave the way for exploring novel 2D magnetism in boron-based magnetic allotropes.

5.
Sci Total Environ ; : 143501, 2020 Nov 11.
Artigo em Inglês | MEDLINE | ID: mdl-33229080

RESUMO

Exogenous silicon has been shown to enhance plant growth and alleviate heavy metals toxicity, but the regulation mechanism of silicon on cadmium migration and transformation in the soil-rice system is still unclear, which is worth further study. In this study, a pot experiment was carried out to explore the influence of different doses (0, 1 and 5%) of mineral silicon on soil properties, nutrient availability, rice growth, soil enzyme activities, Cd bioavailability, and uptake and accumulation of Cd in high-accumulating (H) and low-accumulating (L) rice cultivars grown in contaminated soils. Results showed that mineral-Si treatment could increase the total biomass and grain yield, with an increased rate of 17.7-27.3% and 14.7-19.1% for H; while 26.2-33.4% and 21.3-30.3% for L. Compared with non-mineral-Si treatment, the soil EX-Cd decreased by 3.9-13.3% (H) and 2.3-10.7% (L). Additionally, the Cd content in rice grain was significantly declined by 29.5-31.3% (H) and 34.9-35.2% (L). Mineral-Si enhanced urease, sucrase, and neutral phosphatase activities in both cultivars, but suppressed catalase activity in H. A selective change in bacterial community structure was observed under mineral-Si treatment, however, the bacterial community remained stable, suggesting that the mineral-Si had no adverse effect on the microbial community. The positive response of soil enzymes activities, rice growth and the overall stabilization of microbial environment for mineral-Si addition to the Cd contaminated soils indicated that mineral-Si could mitigate the risk of Cd and well maintain the soil health, proving it to be eco-friendly and low-cost amendment for soils remediation.

6.
J Dig Dis ; 2020 Nov 20.
Artigo em Inglês | MEDLINE | ID: mdl-33215862

RESUMO

Splanchnic vein thrombosis is one of the severe complications of pancreatitis. Until now, no consensus regarding the management of pancreatitis-related splanchnic vein thrombosis has been established. Based on the consensus of multidiscipline experts, this practice guidance systematically reviewed the epidemiology, anatomy, pathophysiology, risk assessment and clinical presentations, and formulated the diagnosis and treatment strategy of pancreatitis-related splanchnic vein thrombosis for providing guidance in clinical practice. This article is protected by copyright. All rights reserved.

7.
Nanoscale ; 12(41): 21291-21298, 2020 Oct 29.
Artigo em Inglês | MEDLINE | ID: mdl-33063799

RESUMO

Out-of-plane ferroelectricity in the two-dimensional (2D) regime shows great promise for applications in next-generation electronic devices but has been rarely reported. Herein, by using high-throughput search and density functional theory (DFT) calculations, three types of ferroelectric MXene phases (type-I: Nb2CS2 and Ta2CS2; type-II: Sc2CO2 and Y2CO2; and type-III: Sc2CS2 and Y2CS2) are for the first time predicted to be achievable by surface functionalization of 2D MXenes. The identified 2D ferroelectric MXenes not only demonstrate remarkable and reversible spontaneous electric polarization along both the out-of-plane and in-plane orientations but also exhibit giant out-of-plane and in-plane piezoelectric response to the external strain. More excitingly, the type-III ferroelectric MXenes possess rare out-of-plane auxeticity that can be triggered by the tensile strain along both the zig-zag and the arm-chair directions. The intriguing ferroelectricity, large piezoelectric response, and auxeticity render the ferroelectric MXenes versatile candidates for nanoscale electronic and mechanic devices.

8.
J Dig Dis ; 2020 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-33128287

RESUMO

OBJECTIVE: This study aimed to determine the risk factors and establish a risk score for post-transjugular intrahepatic portosystemic shunt (TIPS) overt hepatic encephalopathy (OHE). METHODS: Altogether 299 and 62 cirrhotic patients receiving TIPS from January 2015 to March 2018 were divided into the derivation and validation cohorts, respectively. The data of the derivation cohort were analyzed for risk factors of post-TIPS OHE. A risk score was established from the derivation cohort and verified by the validation cohort. RESULTS: During a median follow-up of 112.6 weeks, 52 (17.4%) patients in the derivation cohort experienced post-TIPS OHE. Logistic regression showed that alcoholic cirrhosis (odds ratio [OR] 3.068, 95% confidence interval [CI] 1.423-6.613, P = 0.004), stent diameter of 10 mm (OR 12.046 [95% CI 2.308-62.862], P = 0.003), portal pressure gradient (PPG) decrement ≥60% (OR 3.548 [95% CI 1.741-7.230], P < 0.001), model for end-stage liver disease (MELD) score ≥10 (OR 2.695 [95% CI 1.203-6.035], P = 0.016), blood ammonia (OR 1.009 [95% CI 1.000-1.018], P = 0.043) and notable hydrothorax (OR 4.393 [95% CI 1.554-12.415], P = 0.005) were associated with an increased risk of post-TIPS OHE. The risk score reached a promising risk evaluation of post-TIPS OHE when verified by the validation cohort (sensitivity 71.4%, specificity 70.7%, accuracy 71.0%). CONCLUSIONS: Alcoholic cirrhosis and notable hydrothorax are independent risk factors for post-TIPS OHE in liver cirrhosis, together with the stent diameter of 10 mm, PPG decrement ≥60%, MELD score ≥10 and blood ammonia. The established risk score is reliable to identify high-risk individuals of developing post-TIPS OHE.

9.
Artigo em Inglês | MEDLINE | ID: mdl-33001355

RESUMO

BACKGROUND: Several clinical trials of dapagliflozin in patients with type 2 diabetes mellitus (T2DM) at elevated cardiovascular risk have observed reduced hospitalization for heart failure (HHF). Several studies have also suggested cardiovascular benefits for patients with HF regardless of whether or not they have T2DM. OBJECTIVE: This meta-analysis was conducted to evaluate the therapeutic effects of dapagliflozin in patients with HF. METHODS: The PubMed, Embase, Cochrane Library, and Web of Science databases were systematically searched from database inception to 15 February 2020. Clinical studies of dapagliflozin use in patients with HF were included. Data on HHF, all-cause mortality, cardiovascular death, major adverse cardiovascular events (MACE), systolic blood pressure, body weight, glycated hemoglobin (HbA1c), and adverse events were collected for analysis. RESULTS: Four randomized controlled trials involving 6738 patients with HF were included in this meta-analysis. Patients receiving dapagliflozin showed a significantly lower incidence of HHF [risk ratio (RR) 0.72; P < 0.00001], all-cause mortality (RR 0.83; P = 0.004), cardiovascular death (RR 0.86; P = 0.03), and MACE (RR 0.88; P = 0.03). Moreover, patients receiving dapagliflozin also showed significant improvements in systolic blood pressure and body weight. However, no statistical difference was observed in HbA1c. In addition, hypoglycemia, volume depletion, and renal impairment was not more frequent with dapagliflozin than with placebo. CONCLUSION: This meta-analysis suggests that dapagliflozin could be a therapeutic strategy for patients with HF regardless of the presence or absence of T2DM.

10.
Arch Virol ; 165(12): 3011-3015, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-33025200

RESUMO

The hemagglutinin-esterase (HE) protein of betacoronavirus lineage A is a secondary receptor in the infection process and is involved in the emergence of new betacoronavirus genotypes with altered host specificity and tissue tropism. We previously reported a novel recombinant bovine coronavirus (BCoV) strain that was circulating in dairy cattle in China, but this virus was not successfully isolated, and the genetic characteristics of BCoV are still largely unknown. In this study, 20 diarrheic faecal samples were collected from a farm in Liaoning province that had an outbreak of calf diarrhea (≤ 3 months of age) in November 2018, and all of the samples tested positive for BCoV by RT-PCR. In addition, a BCoV strain with a recombinant HE (designated as SWUN/A1/2018) and another BCoV strain with a recombinant HE containing an insertion (designated as SWUN/A10/2018) were successfully isolated in cell culture (TCID50: 104.25/mL and 104.73/mL, respectively). Unexpectedly, we identified the emergence of a novel BCoV variant characterized by a 12-nt bovine gene insertion in the receptor-binding domain in a natural recombinant HE gene, suggesting a novel evolutionary pattern in BCoV.


Assuntos
Doenças dos Bovinos/epidemiologia , Infecções por Coronavirus/veterinária , Coronavirus Bovino/genética , Diarreia/veterinária , Hemaglutininas Virais/genética , RNA Viral/genética , Proteínas Virais de Fusão/genética , Animais , Bovinos , Doenças dos Bovinos/patologia , Doenças dos Bovinos/virologia , China/epidemiologia , Infecções por Coronavirus/epidemiologia , Infecções por Coronavirus/patologia , Infecções por Coronavirus/virologia , Coronavirus Bovino/classificação , Coronavirus Bovino/isolamento & purificação , Diarreia/epidemiologia , Diarreia/patologia , Diarreia/virologia , Evolução Molecular , Fezes/virologia , Expressão Gênica , Genótipo , Modelos Moleculares , Mutagênese Insercional , Filogenia , Estrutura Secundária de Proteína , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Análise de Sequência de DNA
11.
12.
Theranostics ; 10(22): 10001-10015, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32929330

RESUMO

Angiogenesis enhances cancer metastasis and progression, however, the roles of transcription regulation in angiogenesis are not fully defined. ZNF322A is an oncogenic zinc-finger transcription factor. Here, we demonstrate a new mechanism of Kras mutation-driven ZNF322A transcriptional activation and elucidate the interplay between ZNF322A and its upstream transcriptional regulators and downstream transcriptional targets in promoting neo-angiogenesis. Methods: Luciferase activity, RT-qPCR and ChIP-qPCR assays were used to examine transcription regulation in cell models. In vitro and in vivo angiogenesis assays were conducted. Immunohistochemistry, Kaplan-Meier method and multivariate Cox regression assays were performed to examine the clinical correlation in tumor specimens from lung cancer patients. Results: We validated that Yin Yang 1 (YY1) upregulated ZNF322A expression through targeting its promoter in the context of Kras mutation. Reconstitution experiments by knocking down YY1 under KrasG13V activation decreased KrasG13V-promoted cancer cell migration, proliferation and ZNF322A promoter activity. Knockdown of YY1 or ZNF322A attenuated angiogenesis in vitro and in vivo. Notably, we validated that ZNF322A upregulated the expression of sonic hedgehog (Shh) gene which encodes a secreted factor that activates pro-angiogenic responses in endothelial cells. Clinically, ZNF322A protein expression positively correlated with Shh and CD31, an endothelial cell marker, in 133 lung cancer patient samples determined using immunohistochemistry analysis. Notably, patients with concordantly high expression of ZNF322A, Shh and CD31 correlated with poor prognosis. Conclusions: These findings highlight the mechanism by which dysregulation of Kras/YY1/ZNF322/Shh transcriptional axis enhances neo-angiogenesis and cancer progression in lung cancer. Therapeutic strategies that target Kras/YY1/ZNF322A/Shh signaling axis may provide new insight on targeted therapy for lung cancer patients.

13.
Infect Genet Evol ; 85: 104566, 2020 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-32976973

RESUMO

Caprine kobuvirus (CKoV), a member of the genus Kobuvirus, has only been identified in South Korea and Italy until now. In this study, 24 goat diarrheic fecal samples were collected from 3 farms in Sichuan province, China, and 87.5% (21/24) samples were detected as CKoV positive by RT-PCR. Meanwhile, full-length VP0, VP3, and VP1 genes were simultaneously cloned from 17 clinical samples. Phylogenetic analysis showed that all CKoV strains were most closely related to porcine kobuvirus based on amino acid (aa) sequences of VP0 and VP3 proteins, but CKoV strains were closely related to with Aichivirus B strains (ferret, bovine, and sheep kobuvirus) based on aa sequences of the VP1 protein. Interestingly, compared with known CKoV strains in the GenBank database, Chinese CKoV strains have unique amino acid changes in VP0 and VP1 proteins. Moreover, the first Chinese CKoV nearly complete genome was successfully obtained from a diarrheic fecal sample, named SWUN/F11/2019. Compared with the two known CKoV strains, five aa mutations (S60A, L252I, V267T, I, V 306 L, V331I) were found in the VP0 gene and 7 aa mutations (S57N, G, T243A, V244I, T, A248V, L, S251A, R252H, and M255L) were found in VP1 in the SWUN/F11/2019 genome. This was the first report of the detection and molecular characteristics of CKoV from goats in China, which could be helpful for improving the understanding of the prevalence and genetic evolution of CKoV.

14.
Elife ; 92020 09 09.
Artigo em Inglês | MEDLINE | ID: mdl-32902383

RESUMO

The lateral prefrontal cortex is involved in the integration of multiple types of information, including working memory and motor preparation. However, it is not known how downstream regions can extract one type of information without interference from the others present in the network. Here, we show that the lateral prefrontal cortex of non-human primates contains two minimally dependent low-dimensional subspaces: one that encodes working memory information, and another that encodes motor preparation information. These subspaces capture all the information about the target in the delay periods, and the information in both subspaces is reduced in error trials. A single population of neurons with mixed selectivity forms both subspaces, but the information is kept largely independent from each other. A bump attractor model with divisive normalization replicates the properties of the neural data. These results provide new insights into neural processing in prefrontal regions.

15.
Chem Biol Drug Des ; 2020 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-32896083

RESUMO

Norcantharidin (NCTD), the demethylated analog of cantharidin isolated from Mylabris, is known to inhibit renal fibrosis. However, the underlying mechanism is largely unknown. The present study investigates whether NCTD exerts this effect through regulation of the protein phosphatase 2A catalytic subunit (PP2Ac)-Smad3 pathway. HK-2 human renal proximal tubule cells exposed to transforming growth factor (TGF)-ß1 were used as an in vitro model of renal fibrosis. The levels of total Smad3, C-terminal-phosphorylated Smad3 (p-Smad3), PP2Ac, and fibronectin (Fn) were evaluated by Western blotting. A PP2Ac overexpression plasmid and the PP2Ac inhibitor okadaic acid (OA) were used for functional analyses. The subcellular localization of Smad3 was visualized by immunofluorescence labeling. The results showed that PP2Ac overexpression increased Smad3 phosphorylation and nuclear translocation in HK-2 cells, while pharmacologic inhibition of PP2Ac with OA had the opposite effect. NCTD suppressed Fn and p-Smad3 expression and TGF-ß1-induced nuclear entry of Smad3, but these effects were abrogated by inhibition of PP2Ac. Thus, the anti-renal interstitial fibrosis effect of NCTD is exerted through inhibition of PP2Ac-mediated C-terminal phosphorylation of Smad3. These findings highlight the therapeutic potential of NCTD for the treatment of renal interstitial fibrosis.

16.
Res Sports Med ; 28(4): 572-587, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32954802

RESUMO

The aim of this review was to examine the effects of shoes and insoles on low back pain (LBP). Seven electronic databases were searched from their inception to May 2020. The methodological quality of the 14 included studies was assessed by PEDro scale. Quality of evidence was assessed using GRADE. Moderate evidence on the disability questionnaire score (SMD, 0.52; 95% CI, 0.28 to 0.77; P < 0.001) and pain score (SMD, 0.61; 95% CI, 0.36 to 0.85; P < 0.001) of the custom-made orthotics for chronic LBP compared with no orthotics/insoles intervention was found. Meta-analysis results also showed moderate evidence on the disability questionnaire score (SMD, 0.44; 95% CI, 0.05 to 0.82; P =0.03) in patients who wore unstable shoes compared with regular shoes. Pain and life quality scores showed low-quality evidence of unstable shoes for chronic LBP. Custom-made orthotics and unstable shoes can be recommended to patients as a management option of chronic LBP.

17.
Zhen Ci Yan Jiu ; 45(8): 617-22, 2020 Aug 25.
Artigo em Chinês | MEDLINE | ID: mdl-32869570

RESUMO

OBJECTIVE: To compare the effect of electroacupuncture (EA) of acupoint group for "reinforcing the kidney and regulating Governor Vessel" and acopoint group for "reinforcing the kidney and lung and regulating Governor Vessel" on lear-ning-memory ability and expression of tumor necrosis factor-α (TNF-α) and interleukin-1ß (IL-1ß) proteins in the hippocampus and prefrontal cortex (PFC) in Alzheimer's disease (AD) rats, so as to explore the efficacy of the two acupoint groups and mechanisms underlying improvement of AD. METHODS: Forty male SD rats were randomly divided into control, sham operation, model, "Baihui" + "Shenshu" (GV20+BL23, for "reinforcing the kidney and regulating Governor Vessel") EA and GV20+BL23+ "Feishu" (BL13, GV20+BL23+BL13, for "reinforcing the kidney and lung and regulating Governor Vessel") EA groups (n=8 rats in each group). The AD model was established by bilateral injection of amyloid ß peptide (Aß25-35,10 µL) into bilateral hippocampus, and rats of the sham operation group received injection of normal saline. After successful establishment of the model,EA (2 Hz, 2 mA) was applied to these acupoints for 15 min, once daily for 10 days. Then, the learning-memory ability was assessed by using Morris water maze tests, and the expression levels of TNF-α and IL-1ß proteins in the PFC and hippocampus tissues were detected by using Western blot. RESULTS: Following modeling, the average escape latency of place navigation test were significantly increased (P<0.05) and the platform crossing times of spatial probe test was significantly decreased in the model group than in the control and sham operation groups (P<0.05). The expression levels of IL-1ß and TNF-α proteins in the PFC and hippocampus were apparently up-regulated in the model group than in the control and the sham operation groups (P<0.000 1, P<0.001, P<0.01). After the intervention, the increase of the average escape latency and expression of IL-1ß and TNF-α in the PFC and hippocampus, and the decrease of space exploration test were revised in both GV20+BL23 EA and GV20+BL23+BL13 EA groups (P<0.05,P<0.01). No significant differences were found between the GV20+BL23 and GV20+BL23+BL13 EA groups in the above mentioned indexes (P>0.05). CONCLUSION: EA of both GV20+BL23 and GV20+BL23+BL13 acupoint can improve learning-memory ability of AD rats, which is associated with their effects in down-regulating the expression of IL-1ß and TNF-α in the PFC and hippocampus to reduce inflammatory reaction. There were no significant differences between the two acupoint groups in the therapeutic effects.


Assuntos
Pontos de Acupuntura , Doença de Alzheimer , Eletroacupuntura , Peptídeos beta-Amiloides , Animais , Hipocampo , Interleucina-1beta , Masculino , Córtex Pré-Frontal , Ratos , Ratos Sprague-Dawley , Fator de Necrose Tumoral alfa
18.
Zhen Ci Yan Jiu ; 45(8): 640-4, 2020 Aug 25.
Artigo em Chinês | MEDLINE | ID: mdl-32869574

RESUMO

OBJECTIVE: To observe the effect of electroacupuncture (EA) on the expression of autophagy mediated PI3K/AKT pathway and sex hormones in polycystic ovary syndrome (PCOS) rats, so as to explore the mechanisms underlying improvement of PCOS anovulatory infertility. METHODS: Six-week-old female Sprague-Dawley rats were randomly divided into control, model and EA groups (n=10 per group). The PCOS model was established by gavage of Letrozole solution (1.0 mg/kg), once daily for 21 consecutive days. Then, EA (2 Hz, 1 mA) was applied to the "Sanyinjiao"(SP6) and "Taichong"(LR3) for 20 min, once a day for 14 successive days. The morphological changes of the ovary were observed after H.E. staining. The contents of serum androgen (T), luteinizing hormone (LH) and anti-mullerian hormone (AMH) were detected by ELISA. The expression levels of phosphatidylinositol 3-kinase (PI3K), protein kinase B (AKT) and microtubule-associated protein 1 light chain 3 (LC3) in the ovary tissues were detected by Western blot. RESULTS: Compared with the control group, the levels of serum T, LH and AMH contents, and ovarian LC3 Ⅱ expression and the ratio of LC3 Ⅱ/Ⅰwere significantly increased (P<0.05), and the expression levels of PI3K and AKT proteins were considerably decreased in the model group (P<0.05). After EA intervention, the levels of serum T, LH, AMH, and the ratio of LC3 Ⅱ/Ⅰwere considerably down-regulated (P<0.05), and those of PI3K and AKT were obviously up-regulated in the EA group (P<0.05). CONCLUSION: EA intervention can reduce serum T, LH and AMH contents, and the ratio of ovarian LC3 Ⅱ/Ⅰ and up-regulate ovarian PI3K and AKT in PCOS rats, which may contribute to its effect in improving anovulatory infertility by suppressing autophagy.


Assuntos
Eletroacupuntura , Síndrome do Ovário Policístico , Pontos de Acupuntura , Animais , Autofagia , Feminino , Hormônios Esteroides Gonadais , Fosfatidilinositol 3-Quinases , Ratos , Ratos Sprague-Dawley
19.
Medicine (Baltimore) ; 99(30): e21316, 2020 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-32791722

RESUMO

BACKGROUND: With the change of people's life style, many more people are suffering from obese type 2 diabetes mellitus (T2DM). Acupoint catgut embedding is one of the acupuncture treatment principles in traditional Chinese medicine, which is widely used in the treatment of obese T2DM. However, there is no systematic review of the therapeutic effect of acupoint catgut embedding on obesity T2DM. Therefore, this article aims at the meta-analysis of acupoint catgut embedding in the treatment of obese T2DM, to clarify its curative effect. METHODS: A structured and systemic literature search was conducted in the following databases up to December 1, 2019: PubMed, Cochrane Central Register of Controlled Trials (CENTRAL), Web of Science, EMBASE, CNKI, Wanfang Database. We will use the Review Manager 5.3 software provided by Cochrane collaborative network for statistical analysis. Then we assessed the quality and risk of the included studies and observed the outcome measures. RESULTS: This meta-analysis will further determine the beneficial efficacy of acupoint catgut embedding on obesity T2DM. CONCLUSION: The purpose of this meta-analysis is to explore the effect of acupoint catgut embedding intervention on obese T2DM patients, and provide more options for clinicians and patients to treat obese T2DM. ETHICS AND DISSEMINATION: This systemic review will evaluate the efficacy and safety of acupoint catgut embedding in the treatment of obesity T2DM. Since all the data included are published, the systematic review does not need ethical approval. REGISTRATION NUMBER: CRD42020160801.


Assuntos
Pontos de Acupuntura/classificação , Terapia por Acupuntura/tendências , Categute/efeitos adversos , Diabetes Mellitus Tipo 2/terapia , Obesidade/terapia , Bases de Dados Factuais , Diabetes Mellitus Tipo 2/epidemiologia , Feminino , Humanos , Estilo de Vida , Masculino , Medicina Tradicional Chinesa , Obesidade/complicações , Avaliação de Resultados em Cuidados de Saúde , Ensaios Clínicos Controlados Aleatórios como Assunto , Segurança , Resultado do Tratamento
20.
Sci Rep ; 10(1): 12848, 2020 07 30.
Artigo em Inglês | MEDLINE | ID: mdl-32733103

RESUMO

Robotic rectal cancer resection with natural orifice extraction is a recently developed minimally invasive surgery used in the treatment of patients with rectal cancer. However, its safety and feasibility remain undiscussed and controversial. This study reported the clinical outcomes and prognostic factors pertaining to traditional robotic assisted rectal cancer resection alone against that of robotic rectal cancer resection with natural orifice extraction to provide a discussion on this issue. 49 patients who underwent robotic rectal cancer resection with natural orifice extraction and 49 matched patients who underwent conventional robotic assisted rectal cancer resection were systematically analyzed in this study. Regarding the baseline characteristics, after matching, no significant differences were observed between the natural orifice specimen extraction (NOSE) group and the robotic assisted rectal cancer resection (RARC) group. Patients in the NOSE group had a reduced visual analog scale (p < 0.001), passed flatus more quickly (p = 0.002) and suffered less surgical stress than those in the RARC group. Moreover, 4 complications were observed in the NOSE group and 7 complications in the RARC group with no significant difference (p = 0.337) in terms of complications. The two groups had a similar survival outcomes, where the 3-year overall survival (p = 0.738) and 3-year progression-free survival (p = 0.986) were all comparable between the two groups. Histological differentiation and T stage could be regarded as independent prognostic factors for 3-year overall survival and 3-year progression-free survival. Robotic rectal cancer resection with natural orifice extraction is a safe and feasible minimally invasive surgery for patients suffering from rectal cancer as it encompasses considerable several advantages. Histological differentiation and T stage may serve as independent prognostic factors for 3-year overall survival and 3-year progression-free survival.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA