Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 25
Filtrar
Mais filtros










Base de dados
Tipo de estudo
Intervalo de ano de publicação
1.
Huan Jing Ke Xue ; 40(2): 904-914, 2019 Feb 08.
Artigo em Chinês | MEDLINE | ID: mdl-30628359

RESUMO

Six field varieties of early rice and late rice were selected as test materials for field experiments to explore the difference in CH4 emissions among different rice varieties, and Static Obscura-Gas Chromatography was used to determine the CH4 gas. The results demonstrated a significant difference in the CH4 emissions flux between early and late rice varieties. The average yield of total fertility CH4 emissions was highest in Xiangzaoxian 24 and lowest in Zhuliangyou 819, with a difference of 34.6%. Of the late rice varieties, Tyou 15 was the highest and the Ziyou 299 was the lowest, with a difference of 33.9%. Differences in CH4 emissions and the greenhouse effect of unit yields between different double cropping rice varieties differed significantly. The cumulative CH4 emissions from early rice varieties ranged from 198.3-303.44 kg·hm-2, and the lowest emissions were from Zhuliangyou 819. The greenhouse effect per yield ranged from 0.67 to 1.40 kg·kg-1, and Luliangyou 996 had the lowest emission value. The late-season rice varieties exhibited significantly higher cumulative CH4 emissions compared to early rice, ranging from 291.93 to 388.28 kg·hm-2, and Ziyou 299 had the lowest emission value. The greenhouse effect of per yields rice varieties, while the late rice varieties were contrary to early rice. Reducing carbon and nitrogen concentrations in the rhizosphere and increasing Eh values could reduce CH4 emissions.


Assuntos
Metano/análise , Oryza/crescimento & desenvolvimento , Rizosfera , Agricultura , Efeito Estufa
2.
Mol Ther Nucleic Acids ; 12: 578-590, 2018 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-30195794

RESUMO

Doxorubicin is a widely used anthracycline-based anti-tumor agent for both solid and liquid tumors. Mounting evidence has demonstrated that microRNAs (miRNAs) are involved in chemoresistance and tumorigenesis. However, the roles of microRNA-501-5p (miR-501) in doxorubicin resistance and gastric cancer cell proliferation and invasion are still not fully understood. In this study, we identified that BLID (BH3-like motif-containing protein, cell death inducer) was directly regulated by miR-501 at the post-transcriptional level in multiple gastric cancer cell lines. Endogenous miR-501 was higher, whereas BLID was lower, in doxorubicin-resistant gastric cancer SGC7901/ADR cells compared with their parental SGC7901 cells. miR-501 suppressed gastric cancer cell apoptosis, induced resistance to doxorubicin, and enhanced cell proliferation, migration, and invasion. Subcutaneous injection of miR-501 lentivirus-infected SGC7901 cells resulted in rapid growth of xenograft tumors and resistance to doxorubicin treatment, unlike injection of negative miRNA lentivirus-infected SGC7901 cells. This is achieved at least partially by directly targeting BLID and subsequent inactivation of caspase-9 and caspase-3 and phosphorylation of Akt. Taken together, miR-501 induces doxorubicin resistance and enhances the tumorigenesis of gastric cancer cells by suppressing BLID. miR-501 might be a potential target for doxorubicin resistance and gastric cancer therapy.

3.
Tumour Biol ; 37(1): 911-24, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26256045

RESUMO

We unveiled the association of Annexin A7 with vascular endothelial growth factor-C (VEGF-C) and the effect of upregulation of Annexin A7 in Hca-F and Hca-P cells on inhibiting hepatocarcinoma (HCC) lymph node metastasis (LNM) in vitro and in vivo. A total of 200 inbred 615 mice were randomly divided into four equal groups inoculated with Hca-F, Hca-P, FAnxa7-upregulated, and PAnxa7-upregulated cells, respectively. The primary tumor, popliteal, inguinal, and iliac lymph nodes were prepared for immunohistochemical (IHC) staining, real-time quantitative polymerase chain reaction (qRT-PCR) analysis, Western blot, and hematoxylin-eosin (H&E) staining. There was over 50 % increase both in the number of FAnxa7-upregulated and PAnxa7-upregulated cells migrated through the filter compared to their controls (FAnxa7-control, Hca-F and PAnxa7-control, Hca-P). However, no significant differences were noted in invasion ability between them (all P > 0.05). Tumor lymph vessels were significantly reduced in FAnxa7-upregulated and PAnxa7-upregulated tumors when compared with Hca-F and Hca-P tumors (all P < 0.05). Blood vessel density did not differ significantly between FAnxa7-upregulated and PAnxa7-upregulated tumors and Hca-F and Hca-P tumors. Enzyme-linked immunosorbent assay (ELISA) for VEGF-C showed that upregulating Annexin A7 decreased VEGF-C secretion in FAnxa7-upregulated and PAnxa7-upregulated cells (P < 0.05). The IHC staining result showed that the level of serum Annexin A7 was found to be statistically higher in all experimental groups than that in the control group (P < 0.05). The present results indicated that alterations in serum Annexin A7 expression may be of prognostic relevance in HCC lymphatic metastasis.


Assuntos
Anexina A7/metabolismo , Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/patologia , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/patologia , Animais , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Ensaio de Imunoadsorção Enzimática , Feminino , Imuno-Histoquímica , Metástase Linfática , Masculino , Camundongos , Invasividade Neoplásica , Metástase Neoplásica , Prognóstico , Regulação para Cima , Fator C de Crescimento do Endotélio Vascular/metabolismo
4.
Yao Xue Xue Bao ; 49(1): 131-5, 2014 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-24783518

RESUMO

To investigate the cell proliferation inhibition and apoptosis induced by berberine a-hydroxy f-decanoylethyl sulfonate (HB) on MDA-MB-231 cells in vitro, and the inhibitory effect of HB on the expression of poly adenosine diphosphate RNA polymerase (PARP), MTT assay was used to detect the viability of MDA-MB-231 cells and cell cycle was examined by flow cytometry. The results showed that HB could significantly inhibit the proliferation of MDA-MB-231 cells, and mildly arrested cell cycle progression at S phase. The IC50S for 24, 48 and 72 h treatment were 4.65, 1.46 and 0.75 mg.L-1 (7.55, 2.37 and 1.22 micromol.L-1), respectively. Annexin V-FITC/PI double staining assay showed that HB increased apoptotic ratio of MDA-MB-231 cells. Western blotting analysis showed the expressions of procaspase-3, procaspase-9 and PARP were decreased after HB treatment, while their fragment increased. The results suggest that HB can inhibit the growth and induce apoptosis of MDA-MB-231 cells, which may be associated with inhibition of the expression of procaspase-3, procaspase-9 and PARP.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Apoptose/efeitos dos fármacos , Berberina/análogos & derivados , Neoplasias de Mama Triplo Negativas/patologia , Berberina/farmacologia , Caspase 3/metabolismo , Caspase 9/metabolismo , Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Feminino , Humanos , Concentração Inibidora 50 , Poli(ADP-Ribose) Polimerases/metabolismo , Neoplasias de Mama Triplo Negativas/metabolismo
5.
Clin Chim Acta ; 431: 185-91, 2014 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-24530298

RESUMO

Galectin-3 (Gal-3) plays important roles in cell proliferation, adhesion, differentiation, angiogenesis and apoptosis in normal and pathologic tissues. Accumulated evidences indicate that Gal-3 is closely involved in tumor cell transformation, migration, invasion and metastasis. In this review, the associations of the expression and localization of Gal-3 as well as its potential action mechanism in tumorigenesis in a variety of cancers were summarized and concluded. Gal-3 is gaining its attraction as a potential new biomarker for the diagnosis, treatment and prognosis of certain tumors.


Assuntos
Biomarcadores Tumorais/análise , Galectina 3/genética , Galectina 3/fisiologia , Neoplasias/genética , Galectina 3/análise , Humanos , Neoplasias/metabolismo , Prognóstico
6.
Biomed Pharmacother ; 68(3): 377-84, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24373698

RESUMO

Lymph node metastasis is recognized as an important mode of liver cancer metastasis. Our previous study has built two hepatocarcinoma cell lines, Hca-F with high (75%) and Hca-P with low (25%) incidences of lymph node metastasis, and has indicated that annexin A7 is an important factor in the lymphatic metastasis of tumors. There is evidence that galectin-3 is the binding protein of annexin A7 and works in protein complexes. Our current study shows that both annexin A7 and galectin-3 express higher in Hca-F than Hca-P. Annexin A7 was successfully down-regulated in Hca-P by RNA interference, and this resulted in concomitant reduction of galactin 3 expression in annexin A7 down regulated compared to the control and N-control cells. Using CCK-8 assay, the expression level of annexin A7 and galectin-3 were found to have correlation with the proliferation ability; Transwell assay showed annexin A7 and galectin-3 are involved in cell migration and invasion regulation in mouse hepatocellular carcinoma cell lines, immunofluorescence assay indicate annexin A7 and galectin-3 were co-located annexin A7 and galectin-3 played roles in DNA damage and cell proliferation cycle checkpoint arrest pathway. Those phenomena indicated that annexin A7 influences lymphatic metastasis of tumors by interacting with galectin-3 through the regulation of tumor cell proliferation, attachment, migration and invasion.


Assuntos
Anexina A7/biossíntese , Galectina 3/biossíntese , Neoplasias Hepáticas Experimentais , Animais , Anexina A7/genética , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Galectina 3/genética , Neoplasias Hepáticas Experimentais/metabolismo , Neoplasias Hepáticas Experimentais/patologia , Metástase Linfática , Masculino , Camundongos , Invasividade Neoplásica , Ligação Proteica
7.
Zhonghua Zhong Liu Za Zhi ; 35(5): 337-40, 2013 May.
Artigo em Chinês | MEDLINE | ID: mdl-24054008

RESUMO

OBJECTIVE: To investigate the effect of enoyl coenzyme A hydratase-1 (Ech1) on the proliferation and invasion ability of mouse hepatocarcinoma Hca-P cells in vitro. METHODS: Recombinant pcDNA3.1(+)-Ech1 gene and pcDNA3.1(+) were transfected into Hca-P cells by cationic liposomes introduction. Clone of PEch1 cells that stably expressing Ech1 and clone of control Pvector cells were screened by G418. The Ech1 expression was identified subsequently by reverse transcriptase-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA), respectively. The malignant behaviors of the cell lines were compared by proliferation, invasion and migration test. RESULTS: The cell line Hca-P cells stably expressing Ech1 gene was constructed. The relative expression of Ech1 mRNA in the PEch1 group was 3.21 ± 0.43 and in the Pvector group was 1.44 ± 0.03, with a significant difference between the two groups (P = 0.029). The results of ELISA revealed that the expression of Ech1 protein was 0.140 ± 0.005 in the PEch1 group, 0.088 ± 0.003 in the Pvector group, and 0.078 ± 0.006 in the Hca-P group, showing a significant difference between the PEch1 group and the Pvector and Hca-P groups (P < 0.05). Transwell migration test showed that the number of penetrated cells in the PEch1 group was 143.00 ± 7.25 cells, significantly higher than that of the Pvector group (95.73 ± 3.88 cells) and un-treated Hca-1 group (106.67 ± 3.54 cells, both P < 0.05). The Transwell invasion assay showed that the number of penetrated cells was 77.20 ± 5.46 cells in the PEch1 group, significantly higher than 46.34 ± 4.35 cells in the Pvector group and 49.80 ± 5.21 cells in the un-treated Hca-1 group (both P < 0.05). CONCLUSIONS: The results showed that overexpressed Ech1 in Hca-P cells may significantly increase the cell proliferation in a time-dependent manner. The up-regulation of Ech1 may increase to some extent the migration and invasion capacity of Hca-P cells. The efforts aiming at up-regulation of Ech1 expression may become a therapeutic target in the treatment of hepatocarcinoma.


Assuntos
Isomerases de Ligação Dupla Carbono-Carbono/metabolismo , Movimento Celular , Proliferação de Células , Neoplasias Hepáticas Experimentais , Animais , Isomerases de Ligação Dupla Carbono-Carbono/genética , Neoplasias Hepáticas Experimentais/enzimologia , Neoplasias Hepáticas Experimentais/patologia , Camundongos , Invasividade Neoplásica , Plasmídeos , RNA Mensageiro/metabolismo , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Transfecção , Células Tumorais Cultivadas , Regulação para Cima
8.
Biomed Pharmacother ; 67(4): 251-9, 2013 May.
Artigo em Inglês | MEDLINE | ID: mdl-23540285

RESUMO

In the tumor malignancy progression, lymph node metastasis (LNM) is recognized as an important factor. In this study, RNA interference (RNAi) was employed to down-regulate ANXA7 gene in Hca-F cells, a hepatocarcinoma cell line with high LNM rate. There was no significant effect on cell proliferation ability, but cell division, motility, and invasion abilities were markedly inhibited. By contrast, up-regulating the expression of ANXA7 gene in Hca-P cells with lower LNM rate, cell migration ability was improved and the percentage of cells in S phase was significantly decreased in vitro. Here, we reported that the expression of Ech1, GSN and JNK1 genes, which were relevant to tumor lymphatic metastasis, had been inhibited due to down-regulation ANXA7 gene and promoted due to up-regulation ANXA7 gene by western blot analysis. These results indicated that ANXA7 is a critical factor in the development of lymphatic metastasis in hepatocarcinoma progression.


Assuntos
Anexina A7/genética , Carcinoma Hepatocelular/patologia , Regulação Neoplásica da Expressão Gênica , Neoplasias Hepáticas/patologia , Animais , Western Blotting , Isomerases de Ligação Dupla Carbono-Carbono/genética , Carcinoma Hepatocelular/genética , Linhagem Celular Tumoral , Movimento Celular/genética , Proliferação de Células , Regulação para Baixo , Gelsolina/genética , Neoplasias Hepáticas/genética , Metástase Linfática , Camundongos , Proteína Quinase 8 Ativada por Mitógeno/genética , Invasividade Neoplásica/genética , Interferência de RNA , Regulação para Cima
9.
Zhonghua Gan Zang Bing Za Zhi ; 20(8): 605-10, 2012 Aug.
Artigo em Chinês | MEDLINE | ID: mdl-23207155

RESUMO

To examine the differential expression pattern of Ech1 protein in mouse Hca-F and Hca-P hepatocarcinoma cell lines with high and low rates of lymphatic metastasis, respectively, and to investigate the relationships between Ech1 expression and adhesion of Hca-F cells. Fluorescence two-dimensional difference in-gel electrophoresis (2D DIGE) and mass spectrometry were used to detect Ech1 expression. Ech1 gene silencing was achieved by stable transfection of Hca-F cells with a plasmid vector harboring short hairpin RNA (shRNA) targeting Ech1, pGPU6/GFP/Neo-shRNA-Ech1. Ech1 mRNA and protein expressions were detected by real-time quantitative polymerase chain reaction (qRT-PCR) and Western blotting analysis, respectively. Adhesive properties of cells were assessed by hematoxylin-eosin staining and fluorimetric detection of extracellular matrix (ECM) proteins. Endogenous Ech1 protein level was remarkably higher in the highly metastatic Hca-F cell line than in the Hca-P cell line (2.7-fold by 2D DIGE; 1.5-fold by Western blotting). shRNA-induced silencing of Ech1 significantly reduced the adhesion ability of Hca-F cells, as evidenced by decreased absorbance values of fibronectin and collagen I (Hca-F cells vs. pGPU6/GFP/Neo-shRNA-Ech1 cells: 1.42+/-0.26 vs. 1.01+/-0.27 and 1.14+/-0.07 vs. 0.90+/-0.09, respectively; P less than 0.05). Down-regulation of Ech1 can inhibit the adhesive capacity of metastatic Hca-F cells.


Assuntos
Isomerases de Ligação Dupla Carbono-Carbono/metabolismo , Carcinoma Hepatocelular/patologia , Adesão Celular , Neoplasias Hepáticas/patologia , RNA Interferente Pequeno/genética , Animais , Isomerases de Ligação Dupla Carbono-Carbono/genética , Carcinoma Hepatocelular/enzimologia , Carcinoma Hepatocelular/genética , Linhagem Celular Tumoral , Regulação para Baixo , Eletroforese em Gel Bidimensional , Regulação Neoplásica da Expressão Gênica , Neoplasias Hepáticas/enzimologia , Neoplasias Hepáticas/genética , Linfonodos/patologia , Metástase Linfática , Camundongos , Plasmídeos , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , RNA Interferente Pequeno/farmacologia , Transfecção
10.
Biomed Pharmacother ; 66(3): 167-72, 2012 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-22440897

RESUMO

We have previously demonstrated that chloride intracellular channel 1 (CLIC1) is involved in the lymphatic metastasis of tumors. In this study, a self-designed shRNA sequence of mouse CLIC1 gene was synthesized and inserted into a pGPU6/GFP/Neo plasmid, then stably transfected into mouse hepatic carcinoma cell line Hca-F cells to down-regulate the expression of CLIC1 gene. The levels of expression of CLIC1 mRNA and protein were detected by real-time quantitative polymerase chain reaction (qRT-PCR) and western blot (WB) analysis, respectively. The down-regulation of CLIC1 enhanced proliferative activity, increased the ratio of G2/M and decreased percentage of apoptosis. In addition, the capability of migration and invasion decreased significantly. The results indicate that CLIC1 is a critical factor in the development of lymphatic metastasis.


Assuntos
Carcinoma Hepatocelular/genética , Carcinoma Hepatocelular/patologia , Canais de Cloreto/genética , Neoplasias Hepáticas/genética , Neoplasias Hepáticas/patologia , Animais , Apoptose/genética , Divisão Celular/genética , Processos de Crescimento Celular/genética , Linhagem Celular Tumoral , Movimento Celular/genética , Regulação para Baixo , Fase G2/genética , Metástase Linfática , Camundongos , Invasividade Neoplásica/genética , RNA Mensageiro/genética , RNA Interferente Pequeno/genética , Transfecção/métodos
12.
Med Oncol ; 28(4): 966-72, 2011 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-20490718

RESUMO

c-Jun N-terminal kinase (JNK) is located in focal adhesion plaque (FAP). JNK is necessary to growth, morphogenesis, and differentiation of cells; especially JNK1 has a close relation with tumors. In this study, we silenced JNK1 by using short hairpin RNA (shRNA) and examined the effect on migration and invasion of mouse hepatocellular carcinoma (HCC) cell line Hca-F in vitro. Three shRNA expression vectors (JNK1shRNA-1, JNK1shRNA-2, and JNK1shRNA-3) were constructed and transfected to Hca-F cells stably. The most effective shRNA was selected by detecting the expression levels of mRNA and protein. Transwell assay was performed to detect the ability of migration and invasion of cells. A negative control sequence (JNK1shRNA control) and non-transfected normal Hca-F cells were treated as control groups. The "Results" showed that the expression vectors of pSilencer-JNK1shRNA were constructed and transfected to Hca-F cells successfully. The most effective shRNA was JNK1shRNA-2. The expressions of mRNA and protein of JNK1 in Hca-F cells after transfection of JNK1shRNA-2 were decreased significantly compared with the other groups (all, P<0.01; all, P<0.05). The ability of migration and invasion was decreased after down-regulation of JNK1 expression (all, P<0.05). These results suggest that the inhibition of JNK1 expression can decrease ability of migration and invasion of mouse hepatocellular carcinoma cell line in vitro. JNK1 plays an important role in lymphatic metastasis of HCC. It may be a new target for gene therapy of lymphatic metastasis of HCC.


Assuntos
Carcinoma Hepatocelular/patologia , Neoplasias Hepáticas/patologia , Proteína Quinase 8 Ativada por Mitógeno/biossíntese , Animais , Western Blotting , Carcinoma Hepatocelular/enzimologia , Carcinoma Hepatocelular/genética , Movimento Celular/genética , Inativação Gênica , Neoplasias Hepáticas/enzimologia , Neoplasias Hepáticas/genética , Masculino , Camundongos , Proteína Quinase 8 Ativada por Mitógeno/genética , Invasividade Neoplásica/genética , RNA Interferente Pequeno , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transfecção
13.
Zhonghua Bing Li Xue Za Zhi ; 40(12): 830-3, 2011 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-22336209

RESUMO

OBJECTIVE: To study the expression of enoyl CoA hydratase 1 (ECH1) and the effect when down-regulation of ECH1 gene expression in mouse hepatocarcinoma cell. METHODS: Immunofluorescence was used for detecting the expression of ECH1, and stably transfected Hca-F cells with pGPU6/GFP/Neo-shRNA-ECH1 expression plasmids. Cell proliferation was assessed by Cell counting kit-8 (CCK8) assay. The Boyden-transwell assay (8 µm pore size) was performed to analyze the inhibitory effect of shRNA on Hca-F cell migration and invasion. RESULTS: ECH1 expression was obtained in the cytoplasm and upregulated expression in Hca-F cells than that in Hca-P cells. The down-regulation of ECH1 could inhibit the cell proliferation of Hca-F cells, decrease the number of cell pass through Transwell (27.07 ± 17.49) compared with scramble-negative (72.38 ± 18.83) and Hca-F controls (59.06 ± 30.33), decrease the migration capacities of Hca-F cells, increase the ratio of Hca-F cells in S phase (86.1%) compared with scramble-negative (75.8%) and Hca-F controls (66.2%) and decrease the ratio of G(1) phase (9.4%) compared with scramble-negative (24.2%) and Hca-F controls (30.3%). CONCLUSION: ECH1 serves as a potential critical factor attributes to tumor lymphatic metastasis.


Assuntos
Movimento Celular , Proliferação de Células , Enoil-CoA Hidratase/metabolismo , Neoplasias Hepáticas Experimentais/patologia , RNA Interferente Pequeno/genética , Animais , Ciclo Celular , Linhagem Celular Tumoral , Citoplasma/enzimologia , Regulação para Baixo , Enoil-CoA Hidratase/genética , Regulação Neoplásica da Expressão Gênica , Neoplasias Hepáticas Experimentais/enzimologia , Metástase Linfática , Camundongos , Plasmídeos , Transfecção
14.
Zhonghua Bing Li Xue Za Zhi ; 39(7): 463-6, 2010 Jul.
Artigo em Chinês | MEDLINE | ID: mdl-21055175

RESUMO

OBJECTIVE: To study the localization and expression of CLIC1 in mouse hepatocarcinoma ascites cell lines with different metastatic potentials. METHODS: Mouse hepatocarcinoma ascites models (a high potential of lymphatic metastasis cell line-Hca-F, and a low potential of lymphatic metastasis cell line-Hca-P) were investigated using fluorescent two-dimensional difference-gel electrophoresis (2-D DIGE) and mass spectrometry for detecting the localization and expression of CLIC1. Immunofluorescence, immunocytochemistry and Western blot were used to assess CLIC1 protein status in the two cell lines. RESULTS: CLIC1 expression was obtained in the cytoplasm and plasma membrane of cells in both cell lines. 2-D DIGE showed that CLIC1 was overexpressed in Hca-F cells, 1.6 folds higher than that of the Hca-P cells. Hca-F cells also had a higher integral membrane CLIC1 in the Hca-P cells. CONCLUSIONS: Although CLIC1 expression is detected in both Hca-F and Hca-P cell lines, a higher protein expression level is present in Hca-F cells. CLIC1 may play an important role in tumor metastasis.


Assuntos
Ascite/metabolismo , Canais de Cloreto/metabolismo , Neoplasias Hepáticas Experimentais/metabolismo , Animais , Ascite/patologia , Western Blotting , Linhagem Celular Tumoral , Membrana Celular/metabolismo , Citoplasma/metabolismo , Imuno-Histoquímica , Neoplasias Hepáticas Experimentais/patologia , Metástase Linfática , Camundongos , Camundongos Endogâmicos , Eletroforese em Gel Diferencial Bidimensional
15.
Zhonghua Gan Zang Bing Za Zhi ; 18(2): 131-5, 2010 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-20196953

RESUMO

OBJECTIVE: To study the effects of silencing CLIC1 gene expression on the proliferation and invasion of Hca-F cells. METHODS: The mouse CLIC1 cDNA sequence was retrieved from NCBI. Three shRNA sequences were designed and cloned into pGPU6/GFP/Neo plasmids. The plasmids were transfected into Hca-F cells with Lipofectamine 2000. Cell Counting-8 (CCK-8) kit and transwell chamber were used to study the effects of CLIC1 on the proliferation and invasion of Hca-F cells. RESULTS: The pGPU6/GFP/Neo-shRNA-3 plasmid effectively repressed the expression of CLIC1 mRNA. Inhibition of CLIC1 gene expression led to decreased cell proliferation and reduced invasion. CONCLUSION: CLIC1 is essential for the proliferation and invasion of Hca-F cells.


Assuntos
Carcinoma Hepatocelular/patologia , Proliferação de Células , Canais de Cloreto/genética , Neoplasias Hepáticas/patologia , Interferência de RNA , Animais , Carcinoma Hepatocelular/metabolismo , Linhagem Celular Tumoral , Canais de Cloreto/antagonistas & inibidores , Canais de Cloreto/metabolismo , Regulação Neoplásica da Expressão Gênica , Vetores Genéticos , Neoplasias Hepáticas/metabolismo , Camundongos , Invasividade Neoplásica , Plasmídeos/genética , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , RNA Interferente Pequeno/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transfecção
16.
World J Gastroenterol ; 15(48): 6117-22, 2009 Dec 28.
Artigo em Inglês | MEDLINE | ID: mdl-20027687

RESUMO

AIM: To investigate the possible correlation between osteoglycin expression and gelatinase activity of mouse hepatocarcinoma Hca-F cells. METHODS: A eukaryotic expression plasmid pIRESpuro3 osteoglycin(+) was constructed and transfected into Hca-F cells to investigate the possible correlation between osteoglycin expression and gelatinase activity of Hca-F cells cultured with extract of lymph node, liver, spleen or in DMEM medium. The activity of gelatinases was examined through zymographic analysis. RESULTS: High expression of osteoglycin attenuated the gelatinase activity of Hca-F cells cultured with extract of lymph node, and at the same time, decreased the metastatic potential of Hca-F cells to peripheral lymph nodes in vivo. CONCLUSION: High expression of osteoglycin decreases the gelatinase activity of Hca-F cells cultured with extract of lymph node; regulation of gelatinase activity might be one of mechanisms that osteoglycin contributes to lymphatic metastasis suppression.


Assuntos
Carcinoma Hepatocelular/enzimologia , Gelatinases/metabolismo , Peptídeos e Proteínas de Sinalização Intercelular/metabolismo , Neoplasias Hepáticas/enzimologia , Animais , Linhagem Celular Tumoral , Metástase Linfática , Masculino , Camundongos , Camundongos Endogâmicos , RNA Mensageiro/metabolismo , Transfecção
17.
Zhonghua Bing Li Xue Za Zhi ; 38(6): 376-9, 2009 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-19781342

RESUMO

OBJECTIVE: To study the expression of COX-2 and pregnancy associate plasma protein A (PAPP-A) in coronary arteries and their relationship with acute coronary syndrome. METHODS: Twenty-one autopsy cases with acute coronary syndrome encountered during the period from 2002 to 2007 were enrolled into the study. Another 21 autopsy cases without evidence of acute coronary syndrome were used as the controls. The right and left coronary arteries of each group were dissected, embedded and processed as paraffin sections. Immunohistochemical study for CD68 and alpha-actin was performed to highlight the presence of macrophages and smooth muscle cells, respectively. The expression of COX-2 and PAPP-A was evaluated. RESULTS: In the acute coronary syndrome group, COX-2 was localized mainly in the cytoplasm of endothelial cells, macrophages and smooth muscle cells. COX-2 expression in the cytoplasm of smooth muscle cells (28.60%) was significantly higher than that in the control group (4.76%, chi(2) = 14.13, P< 0.05). There was a positive correlation on COX-2 and PAPP-A expression in smooth muscle cells of the media layer of coronary arteries in acute coronary syndrome group (r = 0.88, P < 0.05). The expression of PAPP-A in smooth muscle cells of the media layer in coronary arteries not associated with plaque formation, was higher than that when there were atherosclerotic plaques (chi(2) = 10.36, P < 0.05). CONCLUSION: In coronary arteries, COX-2 and PAPP-A play certain roles in the pathogenesis of acute coronary syndrome.


Assuntos
Síndrome Coronariana Aguda/metabolismo , Vasos Coronários/metabolismo , Ciclo-Oxigenase 2/metabolismo , Proteína Plasmática A Associada à Gravidez/metabolismo , Síndrome Coronariana Aguda/patologia , Adulto , Idoso , Autopsia , Vasos Coronários/patologia , Feminino , Humanos , Pessoa de Meia-Idade , Miócitos de Músculo Liso/metabolismo , Placa Aterosclerótica/metabolismo , Gravidez , Adulto Jovem
19.
Rapid Commun Mass Spectrom ; 22(20): 3172-8, 2008 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-18798201

RESUMO

The potential biomarkers for the lymphatic metastatic process of mouse hepatocarcinoma were investigated by using two-dimensional difference in-gel electrophoresis (2D DIGE), high-performance liquid chromatography/nano-electrospray ionization tandem mass spectrometry (HPLC/nESI-MS/MS) and GeneChip. 2D DIGE was performed to screen and quantify the differentially expressed proteins between two well-established mouse hepatocarcinoma cell lines, Hca-F with 75% and Hca-P with 25% metastasis rate of lymph node potentials. The protein spots in the gel were visualized by the highly sensitive Deep Purple (GE Healthcare) fluorescent stain. Protein identification was obtained for gel spots by HPLC/nESI-MS/MS analysis with high quality. GeneChip microarray was performed to identify genes differentially expressed at the mRNA level. Seventeen genes including the chloride intracellular channel l, caspase 3, fructose bisphosphatase 2, glutamate dehydrogenase 1, V-crk sarcoma virus CT10 oncogene homolog, N-myc downstream regulated gene1, villin2, gelsolin, enoyl coenzyme A hydratase 1, transketolase, vimentin, annexins A5 and A7, keratin complex2 basic gene7 and gene8, lactamase (bata 2) and Ero1-like protein were found abnormally regulated and expressed concordantly both at the protein and mRNA levels between the two cell lines. More than half of these genes were for the first time revealed to be involved directly in hepatocarcinoma due to the lymphatic metastasis. The interdisciplinary combination of HPLC/nESI-MS/MS with 2D DIGE and GeneChip techniques opens up the possibility for the biomarker discovery of disease with high confidence.


Assuntos
Biomarcadores Tumorais/análise , Metástase Linfática/diagnóstico , Metástase Linfática/genética , Animais , Western Blotting , Linhagem Celular Tumoral , Cromatografia Líquida de Alta Pressão , Eletroforese em Gel Bidimensional , Hidrólise , Processamento de Imagem Assistida por Computador , Indicadores e Reagentes , Camundongos , Camundongos Endogâmicos , Proteínas de Neoplasias/análise , Análise de Sequência com Séries de Oligonucleotídeos , RNA Mensageiro/análise , RNA Mensageiro/biossíntese , RNA Mensageiro/genética , RNA Neoplásico/análise , RNA Neoplásico/biossíntese , RNA Neoplásico/genética , Espectrometria de Fluorescência , Espectrometria de Massas em Tandem , Tripsina
20.
World J Gastroenterol ; 12(42): 6893-7, 2006 Nov 14.
Artigo em Inglês | MEDLINE | ID: mdl-17106944

RESUMO

AIM: To identify genes differentially expressed in mouse hepatocarcinoma ascites cell line with low potential of lymphogenous metastasis. METHODS: A subtracted cDNA library of mouse hepatocarcinoma cell line with low potential of lympho-genous metastasis Hca-P and its synogenetic cell line Hca-F with high metastatic potential was constructed by suppression subtracted hybridization (SSH) method. The screened clones of the subtracted library were sequenced and GenBank homology search was performed. RESULTS: Fifteen differentially expressed cDNA fragments of Hca-P were obtained which revealed 8 known genes, 4 expressed sequence tags (ESTs) and 3 cDNAs showed no homology. CONCLUSION: Tumor metastasis is an incident involving multiple genes. SSH is a useful technique to detect differentially expressed genes and an effective method to clone novel genes.


Assuntos
Carcinoma Hepatocelular/genética , Regulação Neoplásica da Expressão Gênica , Genes Supressores de Tumor , Neoplasias Hepáticas/genética , Metástase Linfática/genética , Animais , Ascite/patologia , Carcinoma Hepatocelular/patologia , Linhagem Celular Tumoral , Desoxirribonucleases de Sítio Específico do Tipo II/metabolismo , Perfilação da Expressão Gênica , Hibridização Genética , Neoplasias Hepáticas/patologia , Camundongos , Camundongos Endogâmicos , RNA Mensageiro/genética , Supressão Genética
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA