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1.
Psychooncology ; 2019 Sep 13.
Artigo em Inglês | MEDLINE | ID: mdl-31518037

RESUMO

BACKGROUND: There is a growing recognition that medical staff members are exposed to job and life stressors that increase the risk of burnout. This study aimed to investigate the potential stressors among medical staff members working at a Cancer Center in Beijing and to explore the demographic, occupational and societal features associated with burnout. METHODS: This was a cross-sectional study. The Maslach Burnout Inventory (MBI) survey was distributed to all medical staff members, along with an anonymous questionnaire to collect general information about demographic, occupational and societal characteristics. The data were analyzed using T test, ANOVA and multivariable linear regression. RESULTS: A total of 1096 of 1208 (91%) medical staff members completed the questionnaires, including 285 (26%) doctors and 572 (52%) nurses. The scores for emotional exhaustion (EE), depersonalization (DP), and personal accomplishment (PA) were 14.51±9.78, 5.78±5.59 and 35.07±10.43, respectively. Domicile, being a nurse, working overtime and low self-rated QoL were predictors of EE; Domicile, being a researcher, low self-rated health, low self-rated QoL and bad colleague relationships were predictors of DP; Age, being a doctor or a nurse, low self-rated health, and low self-rated interpersonal relationships were predictors of low personal accomplishment. CONCLUSION: Compared with the other occupations, doctors and nurses are more likely to experience burnout. Additionally, cultivating a better work environment, promoting the health and quality of life of staff and improving rapport with colleagues may help to prevent burnout.

2.
Artigo em Inglês | MEDLINE | ID: mdl-31512547

RESUMO

Aflatoxins [AFs] are secondary metabolites of the fungus species Aspergillus spp. Both animal and epidemiological studies provided sufficient evidence on the carcinogenic, immunotoxic, mutagenic, and genotoxic potential of AFs. While ingestion is the main route of exposure for AFs through consumption of contaminated food products, agricultural workers and personnel who handle AF-contaminated grains are also at higher risk of exposure via inhalation. The main objective of the review is to provide a comprehensive overview of past scientific studies on occupational exposure to AFs, high-risk occupations, and disease outcomes. A search of peer-reviewed articles was done on PubMed and Web of Science Databases. A total of 164 papers was identified and 61 journal articles were selected for further review. High risk occupations include animal husbandry and processing of grain cereals and/or animal feed. Primary liver cancer and respiratory cancers were the most reported as a result of occupational exposure to AFs. For future studies, improved study designs, better characterization of AFs exposure in an occupational setting, and use of biomarkers are recommended in order to promote better understanding of occupational exposure to AFs and the resulting disease burden among workers.

4.
J Palliat Med ; 22(8): 945-960, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31380727

RESUMO

Background: The World Health Assembly urges members to build palliative care (PC) capacity as an ethical imperative. Nurses provide PC services in a variety of settings, including the home and may be the only health care professional able to access some disparate populations. Identifying current nursing services, resources, and satisfaction and barriers to nursing practice are essential to build global PC capacity. Objective: To globally examine home health care nurses' practice, satisfaction, and barriers, regarding existing palliative home care provision. Design: Needs assessment survey. Setting/Subjects: Five hundred thirty-two home health care nurses in 29 countries. Measurements: A needs assessment, developed through literature review and cognitive interviewing. Results: Nurses from developing countries performed more duties compared with those from high-income countries, suggesting a lack of resources in developing countries. Significant barriers to providing home care exist: personnel shortages, lack of funding and policies, poor access to end-of-life or hospice services, and decreased community awareness of services provided. Respondents identified lack of time, funding, and coverages as primary educational barriers. In-person local meetings and online courses were suggested as strategies to promote learning. Conclusions: It is imperative that home health care nurses have adequate resources to build PC capacity globally, which is so desperately needed. Nurses must be up to date on current evidence and practice within an evidence-based PC framework. Health care policy to increase necessary resources and the development of a multifaceted intervention to facilitate education about PC is indicated to build global capacity.

5.
Chem Biodivers ; 16(8): e1900205, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31294527

RESUMO

The importance of phenolic compounds for responding to various environmental conditions has been widely emphasized. However, the role of interactions between polyphenols and ecological factors, especially C, N, and P stoichiometry was little studied. Here, 15 sites across five provinces of Pinus bungeana in temperate regions of China were studied. The results showed that the higher values of total phenolic contents (TPC) of leaf and litter were distributed among the north distribution area of P. bungeana, lower values were in the south, whereas soil TPC were contrary to leaf and litter TPC. The stepwise regression, path analysis and decision index of path analysis for leaf TPC and ecological factors showed that altitude had the most direct impact on leaf TPC. Moreover, the principal determinants of leaf, litter and soil TPC were soil C/P ratios, longitude, and soil N/P ratios, respectively. In addition, the leaf, litter and soil TPC of P. bungeana were limited by soil C/N ratios, mean annual temperature, and soil P, respectively. Overall, our study provided evidence that ecological factors affected strongly the leaf, litter and soil TPC of P. bungeana.


Assuntos
Pinus/química , Polifenóis/química , Solo/química , Carbono/química , China , Colorimetria , Ecossistema , Nitrogênio/química , Fósforo/química , Pinus/metabolismo , Folhas de Planta/química , Folhas de Planta/metabolismo , Polifenóis/análise , Temperatura Ambiente
6.
Chemosphere ; 234: 232-241, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31220657

RESUMO

Heavy metals, a class of persistent environmental toxicants, are harmful to human health. Cd and Pb are two of the most common toxic heavy metals that have been linked with cancers and malfunction of the nervous system. Notably, contamination of Mn usually coexisted with Cd and Pb in environmental and occupational settings. Studies regularly examined the toxic effects on individual metals; however, potential health and toxic effects of mixtures containing two or more heavy metals are unknown. Here, we investigated toxic effects of Cd, Pb, Mn, and their binary and ternary mixtures in the nematode Caenorhabdities elegans. The toxic outcomes, including effects on growth, reproduction, and feeding, were measured via high-throughput platform analysis. The transgenic strain BY250 with GFP in dopaminergic neurons was used to explore the neurodegenerative effects induced by single metals or their mixtures. The combination index(CI) for mixtures effect was calculated using isobolograms methods. Following the exposure, we found significant toxic effects in C. elegans. For single metals, the toxicity order for growth, reproduction, and feeding were Pb > Cd > Mn. For mixtures, the mixture of Cd + Mn induced a less than addictive effect in C. elegans, whereas the mixtures of Cd + Pb, Pb + Mn, and Cd + Pb + Mn induced greater-than-additive effects. Both single metals and their mixtures induced abnormality in dopaminergic neurons. These results showed combinative toxic and neurodegenerative effects of heavy metal mixtures, and future studies will focus on characterization of concentration-response patterns and identification of potential molecular mechanisms in C. elegans model.

7.
PLoS Negl Trop Dis ; 13(5): e0007396, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-31059500

RESUMO

Strongyloidiasis is a much-neglected but sometimes fatal soil born helminthiasis. The causing agent, the small intestinal parasitic nematode Strongyloides stercoralis can reproduce sexually through the indirect/heterogonic life cycle, or asexually through the auto-infective or the direct/homogonic life cycles. Usually, among the progeny of the parasitic females both, parthenogenetic parasitic (females only) and sexual free-living (females and males) individuals, are present simultaneously. We isolated S. stercoralis from people living in a village with a high incidence of parasitic helminths, in particular liver flukes (Clonorchis sinensis) and hookworms, in the southern Chinese province Guangxi. We determined nuclear and mitochondrial DNA sequences of individual S. stercoralis isolated from this village and from close by hospitals and we compared these S. stercoralis among themselves and with selected published S. stercoralis from other geographic locations. For comparison, we also analyzed the hookworms present in the same location. We found that, compared to earlier studies of S. stercoralis populations in South East Asia, all S. stercoralis sampled in our study area were very closely related, suggesting a recent common source of infection for all patients. In contrast, the hookworms from the same location, while all belonging to the species Necator americanus, showed rather extensive genetic diversity even within host individuals. Different from earlier studies conducted in other geographic locations, almost all S. stercoralis in this study appeared heterozygous for different sequence variants of the 18S rDNA hypervariable regions (HVR) I and IV. In contrast to earlier investigations, except for three males, all S. stercoralis we isolated in this study were infective larvae, suggesting that the sampled population reproduces predominantly, if not exclusively through the clonal life cycles. Consistently, whole genome sequencing of individual worms revealed higher heterozygosity than reported earlier for likely sexual populations of S. stercoralis. Elevated heterozygosity is frequently associated with asexual clonal reproduction.


Assuntos
DNA Ribossômico/genética , RNA Ribossômico 18S/genética , Strongyloides stercoralis/genética , Strongyloides stercoralis/isolamento & purificação , Estrongiloidíase/parasitologia , Animais , China , DNA de Helmintos/genética , Fezes/parasitologia , Feminino , Haplótipos , Humanos , Masculino , Filogenia , Reprodução , Strongyloides stercoralis/fisiologia
8.
Wei Sheng Yan Jiu ; 48(2): 265-268, 2019 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-31133105

RESUMO

OBJECTIVE: To investigate the water iodine content and distribution of drinking-water in Gansu Province, and provide scientific evidence for the implementation of strategies for prevention and treatment of iodine-related diseases. METHODS: The multilevel sampling method was adopted, take the township as sampling unit at the first level, and the administrative village with the median water iodine ≥10 µg/L as the second level sampling unit, different water supply modes(centralized, partial concentration and decentralized water supply) were sampled and the water iodine were detected by recommended iodine deficiency disease test method. RESULTS: A total of 8976 drinking water samples were detected from 1388 towns of 14 cities. the detection range was 0. 0-84. 6 µg/L, the median water iodine was 2. 3 µg/L, and 8301 samples were<10 µg/L, 663 samples were 10~<50 µg/L, 12 samples were ≥50 µg/L, with a ratio of total at 92. 5%, 7. 4% and 0. 1%, respectively. Water iodine content varies between three different water supply method(χ~2=8. 923, P<0. 05), decentralized water iodine was lower than partial concentration water supply(Z=-2. 891, P<0. 01). 71 townships of median water iodine were ≥10 µg/L(5. 1%), the water iodine median range was 10. 1~64. 3 µg/L, 76. 1% of townships were 10-<20 µg/L, 11. 3% were 20-<30 µg/L, 5. 6% were 30-<40 µg/L, 7. 0% were ≥40 µg/L(χ~2=13. 302, P<0. 05). Water samples of 895 administrative villages were detected, the median water iodine was 10. 8 µg/L, 466 administrative villages with a median water iodine ≥10 µg/L, the water iodine range was 10. 0~113. 1 µg/L, the median water iodine was 10. 8 µg/L, Administrative villages of 10-<50 µg/L, 50~<100 µg/L and ≥100 µg/L accounted for 96. 6%, 2. 4% and 1. 0%, respectively. CONCLUSION: Iodine deficiency areas were exist universally in Gansu Province, but there is high iodine point-like distribution areas in the administrative villages. While pay close attention to prevention of Iodine deficiency, the iodine nutrition status monitor in different iodine areas and the iodine supplementation strategy adjusting should be concerned too.


Assuntos
Água Potável/química , Iodo/análise , Abastecimento de Água , China , Cidades , Água Potável/análise , Humanos , Estado Nutricional
9.
Arch Microbiol ; 201(7): 897-906, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-30993372

RESUMO

Humic acids are one of the main organic matters in sediments and contribute importantly to the marine biogeochemical cycles. Extracellular electron transfer is a ubiquitous natural process and has potentials to change the macrostructure of humic acids which can act as an electron shuttle. By setting up marine sediment microbial fuel cells, the present study revealed that enhanced extracellular electron transfer process could increase the content of C and H, but decrease the O content in humic acids, which could result in an increased aromaticity and decreased polarity of humic acids, whereas no significant changes occurred to the humification degree of the humic acids. Specific bacterial groups as potential exoelectrogens including Proteobacteria (especially Pseudomonas strains) and Firmicutes were enriched under enhanced extracellular electron transfer process, indicating that they were active to exchange electrons and might play important roles during the changes of humic acids, while the relative abundance of Verrucomicrobia and Bacteroidetes was reduced during these processes. The results of the present research shed lights on the relation between exoelectrogens and the transformation of humic acids in coastal sediment, while the microbial process and mechanisms behind it require further study.


Assuntos
Fenômenos Fisiológicos Bacterianos , Transporte de Elétrons , Sedimentos Geológicos/microbiologia , Substâncias Húmicas , Fontes de Energia Bioelétrica/microbiologia , Sedimentos Geológicos/química , Substâncias Húmicas/análise
10.
Cancer Med ; 8(5): 2074-2084, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-30982232

RESUMO

Genetic testing for germline mutations in BRCA1/2 of patients with breast cancer (BC) is part of routine patient care. However, BRCA1/2 mutations account only for a fraction of familial BC. A custom panel of 22 gene sequencing was performed on each patient. Among the 481 female patients, 135 patients were detected to carry pathogenic (P)/likely pathogenic (LP) mutations (28.1%), which corresponded to 12 different cancer predisposition genes [14.6% (70/481) on BRCA1 gene, 5.0% (24/481) on BRCA2 gene, 8.5% (41/481) on non-BRCA1/2 genes]. Moreover, 24.7% (119/481) of patients had mutation of unknown significance (VUS) in these genes. The most common (8/481) pathogenic mutation is BRCA1 c.5470_5477del, while BRIP1 2392 C > T of patients was detected. All the mutations detected were mainly seen in the homologous recombinant repair pathway. Compared to BRCA2 mutation, BRCA1 mutation is higher in younger female patients (P < 0.01). Some pathogenic mutations were detected in the patients' familiy members without the past history of tumor and 92 novel mutations were detected (31 on BRCA including 2 P, 16 LP, 13 VUS; 61 on non-BRCA1/2 including 9 LP, 52 VUS). The detection rate of BRCA1/2 mutations was higher in patients with three or more cancer family members than those with one or two. However, the difference was not statistically different. The results suggest that multigene panel testing can increase mutation detection rate for high-risk BC patients. Detailed family history can help to categorize new mutations.

11.
J Cosmet Dermatol ; 2019 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-30859684

RESUMO

BACKGROUND: Pachyonychia congenita (PC), a rare autosomal dominant disorder, is featured by significant hypertrophic nail, palmoplantar keratoderma, and plantar pain. It is caused by the mutation of KRT6A, KRT6B, KRT6C, KRT16, or KRT17. AIMS: To identify the gene mutation caused the PC in a Chinese family. PATIENTS/METHODS: Genomic DNA was extracted from peripheral blood samples of five patients and six healthy individuals. Genomic DNA of three patients was sequenced by whole-exome sequencing (WES). Then, exons 6 of KRT16 of all samples were amplified by polymerase chain reaction (PCR), and PCR products were sequenced to identify potential mutations. RESULTS: We identified the proline substitution mutation p.Leu421Pro (c.1262T>C) in the 2B domain of K16 that is associated with PC in a Chinese family. The same mutation was not found in the six healthy individuals of the family. CONCLUSIONS: The mutation found in this study is the first report in China. So far, 25 mutations in KRT16 have been reportedly associated with PC. Twenty-one mutations are located on exon 1, and four mutations on exon 6.

12.
Biomarkers ; 24(4): 379-388, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30821509

RESUMO

Context: Fumonisins (FNs), a group of mycotoxins produced mainly by Fusarium species, are ubiquitous food contaminants, especially for maize. Fumonisin B1 (FB1) caused severe toxicities in farm animals, induced kidney and liver tumours in rodents and is associated with many human adverse health effects, including oesophageal cancer. International Agency for Research on Cancer (IARC) categorizes FB1 as a possible human carcinogen (Group 2B). Inhibition of ceramide synthesis and disruption of sphingolipids metabolism are well studied as the major mechanisms of FB1-induced toxicity. Increases in sphinganine (Sa) and decrease in sphingosine (So) levels and their ratio are validated biomarkers of FB1 effects. Methods: In this study, we measured urinary levels of Sa, So and Sa/So in 284 children aged 1-14 years who consume maize as a staple diet. Exfoliated cells from urine were processed and sphingolipids quantified by High Pressure Liquid Chromatography. Results and conclusions: Sa and So were detectable in 95.07% and 98.94% of samples, respectively. Creatinine adjusted mean levels and standard deviation of Sa, So and Sa/So ratio were 1.23 ± 2.18, 4.99 ± 8.3 and 0.296 ± 0.587 nM. These results further confirmed the findings in studies with human adults, i.e. urinary Sa, So levels and Sa/So ratio are good biomarkers to assess FNs exposure in children.

13.
Biomed Res Int ; 2019: 4379639, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30834264

RESUMO

Objective: To study the protective effect of Echinacoside for 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) induced dopaminergic (DA) neurons injury in the subacute mouse model of Parkinson's disease (PD) and to explore its mechanism of action. Methods: We chose 10 weeks of healthy wild type C57BL/6 male mice, hypodermic MPTP 30 mg/kg/day, five days, to prepare PD subacute mouse model. Behavior indexes of open field test and pole test were applied to examine the function of ECH to PD subacute mice model of PD sample action. The effects of ECH on dopaminergic neurons and astrocyte were examined using Immunohistochemistry including tyrosine hydroxylase (TH) and glial fibrillary acidic protein (GFAP) expression. The total numbers of TH-positive neurons and GFAP-positive cells in the substantia nigra pars compacts (SNpc) and ventral tegmental area (VTA) were obtained stereologically using the optical fractionator method. Enzyme-linked immunosorbent assay (ELISA) method was used to detect the inflammatory cytokines in the serum, including TNF-α (Ttumor necrosis factor alpha) and IFN-γ (interferon gamma). Protein expressions of ionized calcium binding adaptor molecule 1 (IBA-1), TNF-α, Cleaved caspase-3, glial derived neurotrophic factor (GDNF), and phosphorylated and total extracellular signal-regulated kinase (p-ERK and ERK) in the anatomical region of substantia nigra (SN) were tested by protein immunoblot method (i.e., Western blotting). Results: ECH reversed the reduction of total distance in open field test in MPTP-induced PD model mice (P < 0.01), shortened the return time and total time of PD subacute model mice in pole test (P < 0.01, P < 0.05), significantly reversed the reduction of TH positive neurons induced by MPTP (P < 0.05), and reduced the activation of astrocytes (P < 0.05). Meanwhile, ECH significantly inhibited the expression of IBA-1, Cleaved caspase-3, and TNF-α in midbrain of MPTP model mice (P < 0.05, P < 0.05, and P < 0.05) and upregulated the expression of GDNF (P < 0.05). And ECH lowered the level of TNF-α and IFN-γ in serum (P < 0.05, P < 0.05). Conclusion: ECH has protective effects on the MPTP subacute model mice, its mechanism may be through inhibiting activation of microglia and astrocytes, reducing inflammatory reaction and promoting the secretion of neurotrophic factors, and eventually resulting in the reduction of the DA neurons apoptosis.


Assuntos
Glicosídeos/administração & dosagem , Intoxicação por MPTP/tratamento farmacológico , Fármacos Neuroprotetores/administração & dosagem , Doença de Parkinson/tratamento farmacológico , Animais , Proteínas de Ligação ao Cálcio/genética , Caspase 3/genética , Corpo Estriado/efeitos dos fármacos , Corpo Estriado/patologia , Modelos Animais de Doenças , Neurônios Dopaminérgicos/efeitos dos fármacos , Neurônios Dopaminérgicos/patologia , Regulação da Expressão Gênica/efeitos dos fármacos , Proteína Glial Fibrilar Ácida/genética , Humanos , Imuno-Histoquímica , Intoxicação por MPTP/genética , Intoxicação por MPTP/patologia , Camundongos , Proteínas dos Microfilamentos/genética , Microglia/efeitos dos fármacos , Microglia/patologia , Doença de Parkinson/genética , Doença de Parkinson/patologia , Substância Negra/efeitos dos fármacos , Substância Negra/patologia , Fator de Necrose Tumoral alfa/genética , Tirosina 3-Mono-Oxigenase/genética
14.
Antonie Van Leeuwenhoek ; 112(7): 1077-1085, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30729398

RESUMO

A Gram-stain negative, aerobic, rod-shaped, and non-motile bacterium, designated strain CCMM003T, was isolated from a culture of the green alga Ulva prolifera. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CCMM003T belongs to the family Flavobacteriaceae and exhibits a close relationship to Pseudozobellia thermophila DSM 19858T (92.5%). Optimal growth occurred in the presence of 4% (w/v) NaCl, at pH 7.0 and 30 °C. The polar lipids of strain CCMM003T consisted of phosphatidylethanolamine and six unidentified lipids. The predominant isoprenoid quinone was MK-6. The major fatty acids were iso-C15:0, iso-C15:1 G, iso-C17:0 3-OH and summed feature 3 (C16:1ω7c and/or iso-C15:0 2-OH). The DNA G + C content of strain CCMM003T calculated on the basis of the genome sequence was 41.2 mol% and the genome size was 5.9 Mbp. On the basis of data from this polyphasic study, strain CCMM003T is considered to represent a novel genus and species of the family Flavobacteriaceae, for which the name Ulvibacterium marinum gen. nov., sp. nov. is proposed. The type strain is CCMM003T (= MCCC 1K03244T =KCTC 52639T).


Assuntos
Flavobacteriaceae/isolamento & purificação , Água do Mar/microbiologia , Ulva/microbiologia , Técnicas de Tipagem Bacteriana , Composição de Bases , DNA Bacteriano/genética , Ácidos Graxos/química , Ácidos Graxos/metabolismo , Flavobacteriaceae/classificação , Flavobacteriaceae/genética , Flavobacteriaceae/metabolismo , Filogenia , RNA Ribossômico 16S/genética , Ulva/crescimento & desenvolvimento
15.
Parasitol Res ; 118(3): 829-835, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30689051

RESUMO

Cathepsin L-like protease is an important member of the papain-like cysteine protease and plays numerous indispensable roles in the biology of parasitic organisms. In a previous study, we identified a gene encoding a cathepsin L-like protease of Clonorchis sinensis (CsCPL) that was detected in the cercaria, metacercaria, and adult worm stages by immunolocalization, suggesting that this cysteine protease may be important and involved in the development of C. sinensis. In this study, the mature domain of CsCPL (CsCPL-m) was cloned and expressed in the form of inclusion bodies in Escherichia coli. After refolding, the recombinant CsCPL-m displayed optimal protease activity towards Z-Phe-Arg-AMC substrates but not towards Z-Arg-Arg-AMC, and the activity of the protease was inhibited completely by the cysteine protease-specific inhibitors E-64 and IAA, which further demonstrated that CsCPL belongs to the cathepsin L-like cysteine protease family. Recombinant CsCPL-m exhibited considerable activity at temperatures ranging from 28 to 42 °C, with the highest activity observed at 42 °C. Furthermore, recombinant CsCPL-m exhibited activity across a broad range of pH values (pH 4.0-8.0), with an optimal pH of 5.5. The Km and Vmax of the recombinant CsCPL-m towards Z-Phe-Arg-AMC were determined to be 5.71 × 10-6 M and 0.6 µM/min, respectively, at 37 °C and pH 5.5. The recombinant CsCPL-m could degrade BSA and gelatine, but could not degrade human hemoglobin and human immunoglobulin G. These results implied that CsCPL might participate in the catabolism of host proteins for nutrition during the parasitic life cycle of C. sinensis; thus, CsCPL could be used as a potential vaccine antigen and drug target against C. sinensis infection.


Assuntos
Catepsina L/metabolismo , Clonorchis sinensis/enzimologia , Cisteína Proteases/metabolismo , Proteínas Recombinantes/metabolismo , Sequência de Aminoácidos , Animais , Catepsina L/antagonistas & inibidores , Catepsina L/genética , Clonagem Molecular , Cisteína Proteases/genética , Inibidores de Cisteína Proteinase/metabolismo , Escherichia coli/genética , Escherichia coli/metabolismo , Gelatina/metabolismo , Humanos , Dobramento de Proteína , Proteínas Recombinantes/genética , Soroalbumina Bovina/metabolismo
16.
Int J Biol Sci ; 15(1): 169-182, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30662357

RESUMO

Polypeptide N-acetylgalactosaminyl transferase-6 (GALNT6), a member of the N-acetyl-D-galactosamine transferase family, was shown to be over-expression in mammary cancer and could be used as a biomarker. However, its roles and underlying mechanisms in the pathogenesis of breast cancer are still unclear. In this study, we reported that GALNT6 was up-expression in breast cancer, and it was not associated with tumor stage. The expression level of GALNT6 and menopause status was associated with patient survival. Biological function results illustrated that knockdown of GALNT6 inhibited proliferation, migration and invasion of MDA-MB-231 cells, and increased cell apoptosis. Knockdown of GALNT6 in breast cancer cell attenuated the protein expression of PCNA, cyclin D1, C-myc and ß-catenin, and increased the expression of E-cadherin, caspase 3 and cleaved PARP1. Cell fractionation assay showed that knockdown of GALNT6 reduced the levels of ß-catenin and MUC1-C in nucleus. Simultaneously knockdown of GALNT6 and ß-catenin significantly reduced the level of C-myc. Co-IP experiments indicated that GALNT6 interacted with MUC1-N, ß-catenin interacting with MUC1-C in breast cancer cells. Together, our study reveals that GALNT6 promotes tumorigenicity and metastasis through ß-catenin/MUC1-C signaling pathway.

17.
Psychooncology ; 28(2): 343-350, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30569663

RESUMO

PURPOSE: Despite shared cultural values, Mainland China's health care system differs from that of Hong Kong. We compared preferences for psychosocial care in Mainland breast cancer women with their Hong Kong counterparts to determine core preferences for, and correlates of, clinical psychosocial care implementation. METHODS: Two hundred eighty breast cancer patients from 23 hospitals located in 15 provinces across Mainland China were recruited to complete the 55-item Chinese version of the Australian National Health and Medical Research Council's National Breast Cancer Centre assessment. Items ranked by proportions of women endorsing them as "essential" for care were compared with similar rankings by Hong Kong Chinese women with breast cancer. RESULTS: Valid response rate was 83% (231/280). Among 231 breast cancer patients, greater than 40% endorsed 15/55 items as essential for effective psychosocial care. Of the top 10 ranked "repeatable" items, seven items were common to both Chinese and Hong Kong breast cancer women, while of the top 10 ranked "once-only" items, nine were common. Mainland breast cancer women ranked help with anxiety and social roles higher than did their Hong Kong counterparts. Demographic factors significantly associated with psychosocial care needs included ethnicity, age, income source and level, religious beliefs, education level, marital status, residential status, and current therapies. CONCLUSIONS: Chinese breast cancer patients prioritize both disease and treatment information and psychosocially sensitive care, making these core items in comprehensive psychosocial care implementation by clinicians and nurses.

18.
Int J Mol Sci ; 19(11)2018 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-30384505

RESUMO

Organic compounds are often exposed to the environment, and have an adverse effect on the environment and human health in the form of mixtures, rather than as single chemicals. In this paper, we try to establish reliable and developed classical quantitative structure⁻activity relationship (QSAR) models to evaluate the toxicity of 99 binary mixtures. The derived QSAR models were built by forward stepwise multiple linear regression (MLR) and nonlinear radial basis function neural networks (RBFNNs) using the hypothetical descriptors, respectively. The statistical parameters of the MLR model provided were N (number of compounds in training set) = 79, R² (the correlation coefficient between the predicted and observed activities)= 0.869, LOOq² (leave-one-out correlation coefficient) = 0.864, F (Fisher's test) = 165.494, and RMS (root mean square) = 0.599 for the training set, and Next (number of compounds in external test set) = 20, R² = 0.853, qext2 (leave-one-out correlation coefficient for test set)= 0.825, F = 30.861, and RMS = 0.691 for the external test set. The RBFNN model gave the statistical results, namely N = 79, R² = 0.925, LOOq² = 0.924, F = 950.686, RMS = 0.447 for the training set, and Next = 20, R² = 0.896, qext2 = 0.890, F = 155.424, RMS = 0.547 for the external test set. Both of the MLR and RBFNN models were evaluated by some statistical parameters and methods. The results confirm that the built models are acceptable, and can be used to predict the toxicity of the binary mixtures.

19.
Front Immunol ; 9: 2609, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30483267

RESUMO

Although it is well-recognized that inflammation enhances leukocyte bactericidal activity, the underlying mechanisms are not clear. Here we report that PRL2 is sensitive to oxidative stress at inflamed sites. Reduced PRL2 in phagocytes causes increased respiratory burst activity and enhances phagocyte bactericidal activity. PRL2 (Phosphatase Regenerating Liver 2) is highly expressed in resting immune cells, but is markedly downregulated by inflammation. in vitro experiments showed that PRL2 was sensitive to hydrogen peroxide (H2O2), a common damage signal at inflamed sites. In response to infection, PRL2 knockout (KO) phagocytes were hyper activated, produced more reactive oxygen species (ROS) and exhibited enhanced bactericidal activity. Mice with PRL2 deficiency in the myeloid cell compartment were resistant to lethal listeria infection and cleared the bacteria more rapidly and effectively. Moreover, in vitro experiments demonstrated that PRL2 binds to GTPase Rac and regulates ROS production. Rac GTPases were more active in PRL2 (KO) phagocytes than in wild type cells after bacterium infection. Our findings indicate that PRL2 senses ROS at inflamed sites and regulates ROS production in phagocytes. This positive feedback mechanism promotes bactericidal activity of phagocytes and may play an important role in innate anti-bacterial immunity.

20.
Sci Total Environ ; 654: 300-310, 2018 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-30445330

RESUMO

The optical properties of black carbon aerosols (BC) are determined by the particles size and the associated non-BC materials, which may be source-related or modified during secondary processing. The one-year long monitoring of BC was first conducted using a Single Particle Soot Photometer (SP2) from December 2013 to November 2014 in Nanjing, a megacity in the Yangtze River Delta region of China. The seasonal variation in the BC size distribution and mixing state were investigated. There was no apparent systematic variation in the mean BC core mass median diameter between seasons, as these values were 226 ±â€¯12 nm, 217 ±â€¯13 nm, 211 ±â€¯15 nm and 221 ±â€¯12 nm for winter, spring, summer and autumn respectively. The mixing state of BC was quantified as the bulk relative coating thickness (defined as particle size Dp over core size Dc, Dp/Dc), which ranged from 1.05 to 2.65. The BC was found to be significantly more coated in the winter (Dp/Dc = 1.50 ±â€¯0.30) than in other seasons (Dp/Dc = 1.27 ±â€¯0.09, 1.28 ±â€¯0.10, 1.27 ±â€¯0.11 in spring, summer and autumn respectively). Higher levels of coating during the winter may due to the contributions of the primary source (with the highest BC mass loadings between seasons) or secondary processes such as low temperature that facilitated the condensation. It was found that the photochemical process may enhance the coatings on BC in summer. At nighttime, the reduced and stabilized planetary boundary layer and the nighttime secondary formation may also lead to BC becoming well mixed with other components. Moreover, BC was shown to be less coated when the NOx concentration was high. However, during all seasons, the BC coating was strongly correlated with other non-BC particulate mass, which suggests that at higher pollution levels BC was more significantly coated with other existing materials through coagulation or condensation by other secondary species.

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