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5.
Medicine (Baltimore) ; 99(4): e18743, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31977866

RESUMO

BACKGROUND: Acute pancreatitis (AP) is one of the common diseases with increasing incidence in clinical surgery and other gastrointestinal-digestive departments. Despite the rapid development of modern medicine, the overall mortality rate of AP is still high. Xuebijing (XBJ) injection (a traditional Chinese patent medicine) is a potentially effective drug for AP. This study is designed to assess the efficacy and safety of XBJ injection for AP. METHODS: We will extract data and assess methodological quality of included studies from 7 electronic databases from their inception to December 31, 2019. The primary outcomes include the mortality, surgical intervention, systemic inflammatory response syndrome (SIRS), local complications, systemic infections, gastrointestinal symptoms, and normal blood amylase recovery time. The statistical analysis will be performed using RevMan 5.3 software. RESULTS: This study will provide high-quality evidence for the efficacy of XBJ injection as an adjuvant therapy for AP. CONCLUSION: The study will provide the key evidence for clinical doctors and the development of clinical guidelines.


Assuntos
Medicamentos de Ervas Chinesas/administração & dosagem , Pancreatite/tratamento farmacológico , Humanos , Injeções , Metanálise como Assunto , Ensaios Clínicos Controlados Aleatórios como Assunto , Revisões Sistemáticas como Assunto , Resultado do Tratamento
6.
G3 (Bethesda) ; 10(2): 839-848, 2020 02 06.
Artigo em Inglês | MEDLINE | ID: mdl-31792004

RESUMO

A trispecific hybrid, MTP (hereafter called tripsazea), was developed from intergeneric crosses involving tetraploid Zea mays (2n = 4x = 40, genome: MMMM), tetraploid Tripsacum dactyloides (2n = 4x = 72, TTTT), and tetraploid Z perennis (2n = 4x = 40, PPPP). On crossing maize-Tripsacum (2n = 4x = 56, MMTT) with Z perennis, 37 progenies with varying chromosome numbers (36-74) were obtained, and a special one (i.e., tripsazea) possessing 2n = 74 chromosomes was generated. Tripsazea is perennial and expresses phenotypic characteristics affected by its progenitor parent. Flow cytometry analysis of tripsazea and its parents showed that tripsazea underwent DNA sequence elimination during allohexaploidization. Of all the chromosomes in diakinesis I, 18.42% participated in heterogenetic pairing, including 16.43% between the M- and P-genomes, 1.59% between the M- and T-genomes, and 0.39% in T- and P-genome pairing. Tripsazea is male sterile and partly female fertile. In comparison with previously synthesized trihybrids containing maize, Tripsacum and teosinte, tripsazea has a higher chromosome number, higher seed setting rate, and vegetative propagation ability of stand and stem. However, few trihybrids possess these valuable traits at the same time. The potential of tripsazea is discussed with respect to the deployment of the genetic bridge for maize improvement and forage breeding.

8.
Acta Neuropathol ; 139(2): 319-345, 2020 02.
Artigo em Inglês | MEDLINE | ID: mdl-31768670

RESUMO

Parkinson's disease (PD) is the most common neurodegenerative movement disorder and is characterized by the progressive loss of dopaminergic (DA) neurons in the substantia nigra pars compacta (SNc) and the gradual appearance of α-synuclein (α-syn)-containing neuronal protein aggregates. Although the exact mechanism of α-syn-mediated cell death remains elusive, recent research suggests that α-syn-induced alterations in neuronal excitability contribute to cell death in PD. Because the fragile X mental retardation protein (FMRP) controls the expression and function of numerous neuronal genes related to neuronal excitability and synaptic function, we here investigated the role of FMRP in α-syn-associated pathological changes in cell culture and mouse models of PD as well as in post-mortem human brain tissue from PD patients. We found FMRP to be decreased in cultured DA neurons and in the mouse brain in response to α-syn overexpression. FMRP was, furthermore, lost in the SNc of PD patients and in patients with early stages of incidental Lewy body disease (iLBD). Unlike fragile X syndrome (FXS), FMR1 expression in response to α-syn was regulated by a mechanism involving Protein Kinase C (PKC) and cAMP response element-binding protein (CREB). Reminiscent of FXS neurons, α-syn-overexpressing cells exhibited an increase in membrane N-type calcium channels, increased phosphorylation of ERK1/2, eIF4E and S6, increased overall protein synthesis, and increased expression of Matrix Metalloproteinase 9 (MMP9). FMRP affected neuronal function in a PD animal model, because FMRP-KO mice were resistant to the effect of α-syn on striatal dopamine release. In summary, our results thus reveal a new role of FMRP in PD and support the examination of FMRP-regulated genes in PD disease progression.

9.
Food Chem ; 309: 125791, 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-31699554

RESUMO

The aim of this study was to investigate the effect of phosphorylation on the antioxidant activity of (1 → 3)-ß-d-glucan from yeast cell wall. Alkali-insoluble (1 → 3)-ß-d-glucan was extracted from yeast cell wall by an acid-base method. It was found that the purity of the sample was greatly improved after the precipitation was treated with alkali at 90 ℃ and then by acetic acid, which was about 96.5%. Phosphorylated (1 → 3)-ß-d-glucan was prepared. Infrared (IR) spectra and nuclear magnetic resonance spectra (NMR) confirmed the successful introduction of phosphate into glucan. The substitution degree of phosphate was 0.18. The phosphorylated (1 → 3)-ß-d-glucan could significantly increase SOD and CAT contents in serum, liver and brain of mice, and reduce MDA level in serum, liver and brain to a certain extent in vivo. This lays a solid foundation for the research and development of phosphorylated (1 → 3)-ß-d-glucan antioxidant.


Assuntos
Antioxidantes/metabolismo , beta-Glucanas/química , Animais , Antioxidantes/química , Catalase/sangue , Catalase/metabolismo , Parede Celular/química , Parede Celular/metabolismo , Espectroscopia de Ressonância Magnética , Malondialdeído/sangue , Malondialdeído/metabolismo , Camundongos , Fosforilação , Saccharomyces cerevisiae/metabolismo , Espectrofotometria Infravermelho , Superóxido Dismutase/sangue , Superóxido Dismutase/metabolismo
11.
Huan Jing Ke Xue ; 40(10): 4628-4636, 2019 Oct 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854832

RESUMO

Background levels of cadmium (Cd) in carbonate rock areas of Guizhou Province are high. In order to understand the characteristics of Cd in arable soils in these karst areas, the soils in the northern county of Luodian were selected as the study object. By comparing soils in non-karst areas, the distribution characteristics of soil Cd were studied by combining geostatistical analysis and GIS, and the potential ecological risk index and health risk were used to evaluate the potential level of risk to adults and children. The results show that the arable soil Cd content in this karst area is significantly higher than that in non-karst areas (P<0.05), with a geometric mean of 1.33mg·kg-1 and 0.27 mg·kg-1, respectively. In comparison, the geometric mean of soil Cd content in forested land in the same areas is 1.57mg·kg-1 and 0.22 mg·kg-1, respectively. Based on "soil pollution risk control standards", the Cd content of 90% and 22% of arable soil samples was higher than the risk screening value and the risk control value for karst and non-karst areas, respectively. The areas with medium and high Cd values were mainly distributed in the karst areas, presenting medium to strong ecological risks and extremely strong ecological risks, respectively. In contrast, the areas with the lowest Cd concentrations were distributed in the non-karst areas of Jiaoyan, presenting low potential ecological risk. The health risk assessment showed that the non-carcinogenic risk and carcinogenic risk of the three exposure pathways for adults and children in the karst areas are significantly higher than those in the non-karst areas, but the Cd content in the soil does not present non-carcinogenic and carcinogenic risks to local residents. Overall, the problem of Cd pollution in arable soils in the karst area of northern Luodian, Guizhou, is prominent. Therefore, the study of the risks posed by Cd pollution to the soil-plant-human system should be strengthened, and appropriate measures should be taken to prevent and control Cd pollution in the areas at risk.


Assuntos
Metais Pesados , Poluentes do Solo , Adulto , Cádmio , Criança , China , Monitoramento Ambiental , Humanos , Medição de Risco , Solo
12.
Huan Jing Ke Xue ; 40(6): 2895-2903, 2019 Jun 08.
Artigo em Chinês | MEDLINE | ID: mdl-31854684

RESUMO

The karst landforms in Guizhou are widely distributed, and the problem of soil arsenic (As) pollution is prominent in these areas because of the high environmental background levels. In order to study the distribution characteristics of As contents in agricultural soils with high background values of As, representative As polluted regions (typical karst region, semi-karst region) in southwestern Xingyi City of Guizhou Province were selected as the research objects, and the non-karst region served as the control group. Geostatistical analyses were then combined with GIS data to study the spatial variability and pollution conditions of As in agricultural soils. Furthermore, Moran's I statistic was used to analyze the spatial autocorrelation and directional characteristics of As at a small scale in the soil. The results showed that As contents in soils from different geomorphological regions were ranked as follows:typical karst region > semi-karst region > non-karst region. The arithmetic mean value and geometric mean value of As in agricultural soils in the typical karst region were 47.9 mg·kg-1 and 43.3 mg·kg-1, respectively. Meanwhile, the arithmetic mean value and geometric mean value of As in agricultural soils in the semi-karst region were 36.8 mg·kg-1 and 30.1 mg·kg-1, respectively. The As content in agricultural soils from these two regions was significantly higher than the background values of As in Guizhou. In addition, the standard exceedance rates of As in those two regions were 98.5% and 96.7%, respectively, thus demonstrating a high degree of As accumulation. In contrast, the standard exceedance rate of As in the non-karst region was only 6.7%. Among these three afore-mentioned landform types, the results of independent sample T tests showed that there were no significant differences in the content of As between agricultural soil and (natural) soil (P>0.05). The Moran's I coefficient of the As content in agricultural soil was 0.45, and the Z value was 11.61, thus suggesting that there was a significant positive spatial autocorrelation at the small scale (P<0.05), especially in the northeast-southwest direction, and the structural variation was dominant. The As polluted agricultural soils were generally at the slight pollution and mild pollution levels, which accounted for 27.10% and 29.02% of the samples, respectively. However, some regions were at the level of moderate pollution. The non-polluted samples accounted for 41.94% of the samples.

13.
Eur J Prev Cardiol ; : 2047487319889721, 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31744336
14.
Pancreas ; 48(9): 1136-1147, 2019 10.
Artigo em Inglês | MEDLINE | ID: mdl-31593017

RESUMO

OBJECTIVE: The objective of this study was to assess the efficacy and safety of acupuncture plus routine treatment (RT) for acute pancreatitis (AP). METHODS: Literature searches were performed in 8 databases up to October 31, 2018. Randomized controlled trials comparing acupuncture plus RT with RT alone for AP were included. RESULTS: Twelve eligible studies were included finally. The meta-analysis showed that acupuncture plus RT compared with RT alone could significantly improve the total effective rate and gastrointestinal function and reduce the Acute Physiology, Age, Chronic Health Evaluation II score, tumor necrosis factor α count, the time of resuming to diets, and the length of hospital stay. Only 3 of the studies reported adverse events or reactions. CONCLUSIONS: This study suggested that acupuncture combined with RT may be effective for AP. However, more rigorously designed randomized controlled trials are warranted to confirm the current findings.

17.
Colloids Surf B Biointerfaces ; 182: 110324, 2019 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-31288132

RESUMO

Graphene exhibits excellent mechanical strength, electrical conductivity and good biocompatibility, which make it a suitable candidate as a neural interfacing material in regenerative medicine and tissue engineering. Graphene is reported to promote both of neural stem cells (NSCs) proliferation and differentiation. However, the transcriptomes of 2D graphene-regulated NSC differentiation have not yet been investigated. To identify candidate genes, on which graphene may affect, we used next-generation RNA sequencing to analyze the transcriptome of NSCs differentiated for 21 days on a graphene substrate. These NSCs displayed highly enriched and differentially expressed genes compared with traditional cell culture in vitro. Of these, we identified motor protein genes that might regulate NSC differentiation, including cytoplasmic dynein and axonemal dynein genes, Ccdc108, Dnah5, and Dnah11. Furthermore, we analyzed the cell signaling pathway genes that might regulate NSC differentiation, and we constructed a protein-protein interaction network for the genes that are differentially expressed in NSCs on graphene compared to commercial tissue culture polystyrene substrates. We have identified genes potentially regulating the differentiation and migration of NSCs on graphene substrates, and our findings provide mechanistic evidence for the biological activities of graphene, especially in view of graphene-stem cell interactions.


Assuntos
Dineínas do Axonema/genética , Regulação da Expressão Gênica no Desenvolvimento , Grafite/farmacologia , Células-Tronco Neurais/efeitos dos fármacos , Transcriptoma , Animais , Dineínas do Axonema/metabolismo , Diferenciação Celular , Biologia Computacional/métodos , Embrião de Mamíferos , Perfilação da Expressão Gênica , Proteína Glial Fibrilar Ácida/genética , Proteína Glial Fibrilar Ácida/metabolismo , Grafite/química , Hipocampo/citologia , Hipocampo/crescimento & desenvolvimento , Hipocampo/metabolismo , Camundongos , Células-Tronco Neurais/citologia , Células-Tronco Neurais/metabolismo , Poliestirenos/química , Poliestirenos/farmacologia , Cultura Primária de Células , Mapeamento de Interação de Proteínas , Transdução de Sinais , Tubulina (Proteína)/genética , Tubulina (Proteína)/metabolismo
18.
BMJ Open ; 9(7): e029327, 2019 07 04.
Artigo em Inglês | MEDLINE | ID: mdl-31278104

RESUMO

INTRODUCTION: Acupuncture may be effective for acute pancreatitis (AP). This systematic review aims to assess the efficacy and safety of acupuncture as an adjuvant treatment for AP. METHODS AND ANALYSIS: We will search PubMed, the Cochrane Central Register of Controlled Trials, EMBASE, Web of Science, China National Knowledge Infrastructure, Wan Fang Data and Chinese Science Journal Database from inception to 30 June 2019 to identify any eligible study. Only randomised controlled trials will be included. The selection of studies, data extraction and management will be completed by two reviewers independently. The primary outcomes include the overall response rate, mortality during the treatment, the proportion of patients with severe acute pancreatitis transferred to the intensive care unit or scheduled for surgery, gastrointestinal function and the acute physiology and chronic health evaluation II scores. The secondary outcomes include visual analogue scale, the use of analgesics, the recovery time of blood amylase becoming normal, tumour necrosis factor α counts, IL-6 counts, IL-10 counts, length of hospital stay and adverse events related to acupuncture (such as fainting, nausea, haematoma and local infection). Review Manager V.5.3 software will be used for statistical analyses. The risk of bias of included studies will be assessed by the Cochrane 'risk of bias' tool. ETHICS AND DISSEMINATION: This study will not involve personal information. The ethical approval will not be required. The results will be published in a peer-reviewed journal. TRIAL REGISTRATION NUMBER: CRD42018115099.

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