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1.
J Nanobiotechnology ; 19(1): 320, 2021 Oct 13.
Artigo em Inglês | MEDLINE | ID: mdl-34645456

RESUMO

Since the number of raw material selections for the synthesis of carbon dots (CDs) has grown extensively, herbal medicine as a precursor receives an increasing amount of attention. Compared with other biomass precursors, CDs derived from herbal medicine (HM-CDs) have become the most recent incomer in the family of CDs. In recent ten years, a great many studies have revealed that HM-CDs tend to be good at theranostics without drug loading. However, the relevant development and research results are not systematically reviewed. Herein, the origin and history of HM-CDs are outlined, especially their functional performances in medical diagnosis and treatment. Besides, we sort out the herbal medicine precursors, and analyze the primary synthetic methods and the key characteristics. In terms of the applications of HM-CDs, medical therapeutics, ion and molecular detection, bioimaging, as well as pH sensing are summarized. Finally, we discuss the crucial challenges and future prospects.

2.
Pest Manag Sci ; 2021 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-34619015

RESUMO

BACKGROUND: Neuroligin protein (NLG) is a nerve cell adhesion molecule and plays a key role in the precision apposition of presynaptic domains on inhibitory and excitatory synapses. Existing studies mainly focused on the function of NLG3 against the excitatory channel. However, the interaction between insect NLG3 and ionotropic GABA receptor, which is the main inhibitory channel, remains unclear. In this study, the Nlg3 of common cutworm (CCW), Spodoptera litura Fabricius, one important agricultural lepidopteron, is selected to explore its function in inhibitory channel. RESULTS: The SlNlg3 was obtained and the SlNLG3 contains the characteristic features including transmembrane domain, PDZ-binding motif and type-B carboxylesterases signature 2 motif. The SlNlg3 mRNA was most abundant in midgut, and exhibited multiple expression patterns in different developmental stages and tissues or body parts. Compared with the single injection of SlRDL1, the EC50 value of GABA in activating currents was smaller in Xenopus laevis oocytes co-injected with SlRDL1 and SlNlg3. In addition, SlNlg3 could enhance the GABA-induced current of homomeric SlRDL1 channel from -391.86 ± 15.41 nA to -2152.51 ± 30.09 nA. DsSlNlg3 depressed the expression level of SlNlg3 mRNA more than 64.29% at 6 h. After exposure to LD50 of fluralaner, the mortality of CCW injected with dsSlNlg3 was significantly decreased by 13.34% and 30.00% at 24 and 48 h, respectively, compared to injection of dsEGFP. CONCLUSION: NLG3 should have physiological function on ionotropic GABA receptor in vitro, which provided a favorable foundation for further research on the physiological function of Nlg gene in lepidopteron. This article is protected by copyright. All rights reserved.

3.
Se Pu ; 39(10): 1065-1076, 2021 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-34505428

RESUMO

The miniaturization of liquid chromatography equipment is among the most important focus areas in chromatographic technology. It involves the miniaturization of the physical dimensions of the instrument, size of the separation material, and inner diameter of the column. The advantages of a reduced inner diameter of the column have been investigated for several decades, and can be summarized as follows. First, the sample consumption is lower, which is particularly beneficial when a limited amount of sample is available, as is the case with natural products, and in biochemistry and biomedicine. Second, the consumption of the mobile phase is reduced, making the process environmentally friendly and facilitating green chemistry. This allows the addition of more expensive solvent additives, such as chiral additives or isotopic reagents, while maintaining a low analysis cost. Moreover, the degree of band dilution in the column is lower than that with conventional liquid chromatography under the same sample injection conditions. Thus, enhanced mass sensitivity is achieved. Other benefits of a reduced inner diameter of the column include temperature control due to effective heat transfer through the columns and easier coupling to mass detectors, which is particularly advantageous for analyzing complex samples. Typically, the term “nano liquid chromatography” is associated with liquid chromatography, which employs capillary columns of inner diameters less than 100 μm and flow rates in the range of tens to hundreds of nanoliters per minute. Because of the extremely low flow rates and small column volume, the extra-column effect becomes more prominent. Thus, the requirements for every component of liquid chromatographs are augmented toward improving their performance and optimizing the extra-column band broadening of the entire system. The solvent delivery equipment should be able to pump mobile phases accurately and steadily at nanoliter-level flow rates. A gradient mode is required to achieve this, which implies that the lowest flow rate for a single pump unit should reach a few nanoliters per minute. A certain operating pressure is also necessary to employ columns with different inner diameters and particle sizes. A precise and repeatable sample injection procedure is essential for nano liquid chromatography. The injection volume and mode should be suitable for capillary columns, without inducing a significant extra-column effect. A higher-sensitivity detector should be employed, and sample dispersion should be limited. The improved tubing and connection method in nano liquid chromatography should offer stability, reliability, and ease of operation. The extra-column volume should also be restricted to suit nanoliter-level flow rates. Considering that most nano liquid chromatographic instruments have been coupled with a mass detector, this review mainly focused on nanoliter solvent delivery modules, sample injection modules, and tubing and connection modules. By searching and summarizing research articles, technical patents, and brochures of instrument manufacturers, technical routes and research progress on these modules were described in detail. The pump designs can be classified into four types. Pneumatic amplifying pumps have been used in ultra-high-pressure applications. The flow-splitting delivery system, though easy to realize, may lead to a large amount of solvent wastage. Splitless pumps, which are classified based on two main principles, are widely used. Some pumps based on other physical phenomena have been suggested; however, they lacked stability and robustness. Two types of injection modes have been utilized in nano liquid chromatography. The direct nanoliter injection mode typically takes advantage of the groove on the rotor of a switching valve. The trapping injection mode uses trap columns to enable the introduction of large sample volumes. As for the tubing and connection, a few appropriate designs can be acquired from commercial suppliers. The robustness has been improved using some patented technologies. The optimization principles and research progress on optical absorption detection are briefly introduced. Finally, commercial nano liquid chromatographic systems are compared by considering the pumps and injectors.


Assuntos
Reprodutibilidade dos Testes , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Tamanho da Partícula , Solventes
4.
Light Sci Appl ; 10(1): 197, 2021 Sep 24.
Artigo em Inglês | MEDLINE | ID: mdl-34561416

RESUMO

In vivo fluorescence imaging in the second near-infrared window (NIR-II) has been considered as a promising technique for visualizing mammals. However, the definition of the NIR-II region and the mechanism accounting for the excellent performance still need to be perfected. Herein, we simulate the photon propagation in the NIR region (to 2340 nm), confirm the positive contribution of moderate light absorption by water in intravital imaging and perfect the NIR-II window as 900-1880 nm, where 1400-1500 and 1700-1880 nm are defined as NIR-IIx and NIR-IIc regions, respectively. Moreover, 2080-2340 nm is newly proposed as the third near-infrared (NIR-III) window, which is believed to provide the best imaging quality. The wide-field fluorescence microscopy in the brain is performed around the NIR-IIx region, with excellent optical sectioning strength and the largest imaging depth of intravital NIR-II fluorescence microscopy to date. We also propose 1400 nm long-pass detection in off-peak NIR-II imaging whose performance exceeds that of NIR-IIb imaging, using bright fluorophores with short emission wavelength.

5.
J Agric Food Chem ; 69(39): 11582-11591, 2021 Oct 06.
Artigo em Inglês | MEDLINE | ID: mdl-34555899

RESUMO

The ionotropic γ-aminobutyric acid (iGABA) receptor is commonly considered as a fast inhibitory channel and is an important insecticide target. Since 1990, RDL, LCCH3, and GRD have been successively isolated and found to be potential subunits of the insect iGABA receptor. More recently, one orphan gene named 8916 was found and considered to be another potential iGABA receptor subunit according to its amino acid sequence. However, little information about 8916 has been reported. Here, the 8916 subunit from Chilo suppressalis was studied to determine whether it can form part of a functional iGABA receptor by co-expressing this subunit with CsRDL1 or CsLCCH3 in the Xenopus oocyte system. Cs8916 or CsLCCH3 did not form functional ion channels when expressed alone. However, Cs8916 was able to form heteromeric ion channels when expressed with either CsLCCH3 or CsRDL1. The recombinant heteromeric Cs8916/LCCH3 channel was a cation-selective channel, which was sensitive to GABA or ß-alanine. The current of the Cs8916/LCCH3 channel was inhibited by dieldrin, endosulfan, fipronil, or ethiprole. In contrast, fluralaner, broflanilide, and avermectin showed little effect on the Cs8916/LCCH3 channel (IC50s > 10 000 nM). The Cs8916/RDL1 channel was sensitive to GABA, but was significantly different in EC50 and Imax for GABA to those of homomeric CsRDL1. Fluralaner, fipronil, or dieldrin showed antagonistic actions on Cs8916/RDL1. In conclusion, Cs8916 is a potential iGABA receptor subunit, which can interact with CsLCCH3 to generate a cation-selective channel that is sensitive to several insecticides. Also, as Cs8916/RDL1 has a higher EC50 than homomeric CsRDL1, Cs8916 may affect the physiological functions of CsRDL1 and therefore play a role in fine-tuning GABAergic signaling.


Assuntos
Inseticidas , Mariposas , Sequência de Aminoácidos , Animais , Inseticidas/farmacologia , Mariposas/metabolismo , Receptores de GABA/genética , Receptores de GABA/metabolismo , Receptores de GABA-A , Ácido gama-Aminobutírico
6.
Cell Death Dis ; 12(10): 866, 2021 Sep 23.
Artigo em Inglês | MEDLINE | ID: mdl-34556635

RESUMO

Tubules injury and immune cell activation are the common pathogenic mechanisms in acute kidney injury (AKI). However, the exact modes of immune cell activation following tubule damage are not fully understood. Here we uncovered that the release of cytoplasmic spliceosome associated protein 130 (SAP130) from the damaged tubular cells mediated necroinflammation by triggering macrophage activation via miRNA-219c(miR-219c)/Mincle-dependent mechanism in unilateral ureteral obstruction (UUO) and cisplatin-induced AKI mouse models, and in patients with acute tubule necrosis (ATN). In the AKI kidneys, we found that Mincle expression was tightly correlated to the necrotic tubular epithelial cells (TECs) with higher expression of SAP130, a damaged associated molecule pattern (DAMP), suggesting that SAP130 released from damaged tubular cells may trigger macrophage activation and necroinflammation. This was confirmed in vivo in which administration of SAP130-rich supernatant from dead TECs or recombinant SAP130 promoted Mincle expression and macrophage accumulation which became worsen with profound tubulointerstitial inflammation in LPS-primed Mincle WT mice but not in Mincle deficient mice. Further studies identified that Mincle was negatively regulated via miR-219c-3p in macrophages as miR-219c-3p bound Mincle 3'-UTR to inhibit Mincle translation. Besides, lentivirus-mediated renal miR-219c-3p overexpression blunted Mincle and proinflammatory cytokine expression as well as macrophage infiltration in the inflamed kidney of UUO mice. In conclusion, SAP130 is released by damaged tubules which elicit Mincle activation on macrophages and renal necroinflammation via the miR-219c-3p-dependent mechanism. Results from this study suggest that targeting miR-219c-3p/Mincle signaling may represent a novel therapy for AKI.

7.
Interdiscip Sci ; 2021 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-34487327

RESUMO

The rapid advances in sequencing technology have led to an explosion of sequence data. Sequence alignment is the central and fundamental problem in many sequence analysis procedure, while local alignment is often the kernel of these algorithms. Usually, Smith-Waterman algorithm is used to find the best subsequence match between given sequences. However, the high time complexity makes the algorithm time-consuming. A lot of approaches have been developed to accelerate and parallelize it, such as vector-level parallelization, thread-level parallelization, process-level parallelization, and heterogeneous acceleration, but the current researches seem unsystematic, which hinders the further research of parallelizing the algorithm. In this paper, we summarize the current research status of parallel local alignments and describe the data layout in these work. Based on the research status, we emphasize large-scale genomic comparisons. By surveying some typical alignment tools' performance, we discuss some possible directions in the future. We hope our work will provide the developers of the alignment tool with technical principle support, and help researchers choose proper alignment tools.

8.
J Biomed Nanotechnol ; 17(8): 1525-1534, 2021 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-34544530

RESUMO

The development of science and technology has deepened people's understanding of cancer, changing the management of malignant tumors in the medical field. Given the common precancerous characteristics of colorectal cancer (CRC), researchers studied early CRC screening. The complexity of traditional diagnostics forced medical staff to speed up CRC innovation early screening methods. Here, we prepared nano-colloidal gold raw materials with different particle sizes (15 and 30 nm) and observed the morphological characteristics and properties of the materials. Simultaneously, the nanocolloidal gold double antibody sandwich kit was designed through the optimum pH value and protein content screening experiment. The results of clinical enteroscopy confirmed the important guiding significance of the equipment in early CRC screening.


Assuntos
Neoplasias Colorretais , Sangue Oculto , Cromatografia de Afinidade , Neoplasias Colorretais/diagnóstico , Detecção Precoce de Câncer , Coloide de Ouro , Humanos
9.
Chempluschem ; 86(8): 1116-1122, 2021 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-34402212

RESUMO

A series of novel 2,6-functionalized azulene molecules Azu1-3 with varied fluorene substituents at the 1- and 3-positions of azulene as well as at the 5'-position of 2-thiophene group were synthesized. Their electronic absorption and emission spectra at neutral and protonated states were examined. It was found that after functionalization with fluorenyl groups, Azu1-3 exhibited absorption maxima at 445, 451 to 468 nm, respectively. In contrast, their corresponding protonated species showed much redshifted absorption maxima at 560, 582 to 643 nm, respectively, mainly due to the extension of conjugation length and the large dipole moment along the C2v axis of 2,6-substituted azulene molecules. Azu1-3 are non-fluorescent in their neutral forms, but became emissive in their protonated states. Analysis of absorption and emission spectra shows that substitution of the 1- or 3-position of azulene led to decrease in response to trifluoroacetic acid.

10.
Diabetes ; 2021 Aug 10.
Artigo em Inglês | MEDLINE | ID: mdl-34376476

RESUMO

Foot process effacement is an important feature of early diabetic nephropathy (DN) which is closely related to the development of albuminuria. Under certain nephrotic conditions, the integrity and function of the glomerular slit diaphragm (SD) structure were impaired and replaced by the tight junction (TJ) structure, resulting in so-called SD-TJ transition, which could partially explain the effacement of foot processes at the molecular level. However, the mechanism underlying the SD-TJ transition has not been described in DN. Here, we demonstrated that impaired autophagic flux blocked p62 mediated degradation of ZO-1 (TJ protein) and promoted podocytes injury via activation of caspase 3 and caspase 8. Interestingly, the expression of VDR in podocytes was decreased under diabetic condition which impaired autophagic flux through down-regulating Atg3. Of note, we also found that VDR abundance was negatively associated with impaired autophagic flux and SD-TJ transition in the glomeruli from human renal biopsy samples with DN. Furthermore, VDR activation improved autophagic flux and attenuated SD-TJ transition in the glomeruli of diabetic animal models. In conclusion, our data provided the novel insight that VDR/Atg3 axis deficiency resulted in SD-TJ transition and foot processes effacement via blocking p62-mediated autophagy pathway in DN.

11.
Artigo em Inglês | MEDLINE | ID: mdl-34370878

RESUMO

BACKGROUND: Colorectal cancer, as a common malignant carcinoma in the gastrointestinal tract, has a high mortality globally. However, the specific molecular mechanisms of long non-coding RNA (lncRNA) thymopoietin antisense transcript 1 (TMPO-AS1) in colorectal cancer were unclear. METHODS: We tested the expression level of TMPO-AS1 via qRT-PCR in colorectal cancer cells while the protein levels of branched chain amino acid transaminase 1 (BCAT1) and the stemness-related proteins were evaluated by Western blot analysis. Colony formation, EdU staining, TUNEL, flow cytometry and sphere formation assays were to assess the biological behaviors of colorectal cancer cells. Then, luciferase reporter, RIP and RNA pull down assay were applied for confirming the combination between microRNA-98-5p (miR-98-5p) and TMPO-AS1/BCAT1. RESULTS: TMPO-AS1 was aberrantly expressed at high levels in colorectal cancer cells. Silenced TMPO-AS1 restrained cell proliferation and stemness and promoted apoptosis oppositely while overexpressing TMPO-AS1 exerted the adverse effects. Furthermore, miR-98-5p was proven to a target of TMPO-AS1 inhibit cell progression in colorectal cancer. Additionally, BCAT1 was proved to enhance cell progression as the target of miR-98-5p, and it offset the effect of silenced TMPO-AS1 on colorectal cancer cells. CONCLUSION: TMPO-AS1 promotes the progression of colorectal cancer cells via sponging miR-98-5p to upregulate BCAT1 expression.

12.
Nano Res ; : 1-7, 2021 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-34336143

RESUMO

The inferior electrical contact to two-dimensional (2D) materials is a critical challenge for their application in post-silicon very large-scale integrated circuits. Electrical contacts were generally related to their resistive effect, quantified as contact resistance. With a systematic investigation, this work demonstrates a capacitive metal-insulator-semiconductor (MIS) field-effect at the electrical contacts to 2D materials: The field-effect depletes or accumulates charge carriers, redistributes the voltage potential, and gives rise to abnormal current saturation and nonlinearity. On one hand, the current saturation hinders the devices' driving ability, which can be eliminated with carefully engineered contact configurations. On the other hand, by introducing the nonlinearity to monolithic analog artificial neural network circuits, the circuits' perception ability can be significantly enhanced, as evidenced using a coronavirus disease 2019 (COVID-19) critical illness prediction model. This work provides a comprehension of the field-effect at the electrical contacts to 2D materials, which is fundamental to the design, simulation, and fabrication of electronics based on 2D materials. Electronic Supplementary Material: Supplementary material (results of the simulation and SEM) is available in the online version of this article at 10.1007/s12274-021-3670-y.

13.
Biosens Bioelectron ; 193: 113521, 2021 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-34380102

RESUMO

In this work, we investigated the ability of impedance flow cytometry to measure the shape of single cells/particles. We found that the impedance pulses triggered by micro-objects that are asymmetric in morphology show a tilting trend, and there is no such a tilting trend for symmetric ones. Therefore, we proposed a new metric, tilt index, to quantify the tilt level of the impedance pulses. Through simulation, we found that the value of tilt index tends to be zero for perfectly symmetrical objects, while the value is greater than zero for asymmetrical ones. Also, this metric was found to be independent on the trajectories (i.e., lateral, and z-direction shift) of the target micro-object. In experiments, we adopted a home-made lock-in amplifier and performed experiments on 10 µm polystyrene beads and Euglena gracilis (E. gracilis) cells with varying shapes. The experimental results coincided with the simulation results and demonstrated that the new metric (tilt index) enables the impedance cytometry to characterize the shape single cells/particles without microscopy or other optical setups.


Assuntos
Técnicas Biossensoriais , Forma Celular , Impedância Elétrica , Citometria de Fluxo , Poliestirenos
14.
J Transl Med ; 19(1): 355, 2021 08 17.
Artigo em Inglês | MEDLINE | ID: mdl-34404433

RESUMO

BACKGROUND: Diabetic nephropathy (DN) is a leading cause of renal failure, whereas the effective and early diagnostic biomarkers are still lacking. METHODS: Fourteen cytokines and chemokines mRNA were detected in urinary extracellular vesicles (EVs) from the screening cohort including 4 healthy controls (HC), 4 diabetes mellitus (DM) and 4 biopsy-proven DN patients, and was validated in another 16 HC and 15 DM and 28 DN patients. Correlation analysis was performed between the candidate biomarkers and clinic parameters as well as kidney histological changes. The findings were also confirmed in DN rat model with single injection of STZ. RESULTS: The number of small EVs secreted in urine was increased in DN patients compared to DM patients and healthy controls, with expression of AQP1 (a marker of proximal tubules) and AQP2 (a marker of distal/collecting tubules). Small EVs derived CCL21 mRNA increased significantly in DN patients and correlated with level of proteinuria and eGFR. Interestingly, elevated CCL21 mRNA from urine small EVs was observed in DN patients with normal renal function and could discriminate early DN patients from DM more efficiently compared to eGFR and proteinuria. CCL21 also showed an accurate diagnostic ability in distinguishing incipient from overt DN. Histologically, CCL21 mRNA expression increased progressively with the deterioration of tubulointerstitial inflammation and showed the highest level in nodular sclerosis group (class III) in DN patients. Remarkable infiltration of CD3 positive T cells including both CD4 and CD8 positive T cell population were observed in DN patients with high-CCL21 expression. Besides, accumulation of CD3 positive T cells correlated with level of urinary small EVs derived CCL21 and co-localized with CCL21 in the tubulointerstitium in DN patients. Finally, the correlation of CCL21 expression in renal cortex and urinary small EVs was confirmed in STZ-induced DN rat model. CONCLUSIONS: Urinary small EVs derived CCL21 mRNA may serve as early biomarker for identifying DN linked with pathogenesis. CCL21 mRNA mediated T cell infiltration may constitute the key mechanism of chronic inflammation in DN.


Assuntos
Quimiocina CCL21 , Diabetes Mellitus Tipo 2 , Nefropatias Diabéticas , Vesículas Extracelulares , Animais , Aquaporina 2 , Biomarcadores , Quimiocina CCL21/genética , Diabetes Mellitus Tipo 2/complicações , Nefropatias Diabéticas/diagnóstico , Nefropatias Diabéticas/genética , Humanos , RNA Mensageiro/genética , Ratos
15.
Biosensors (Basel) ; 11(8)2021 Aug 04.
Artigo em Inglês | MEDLINE | ID: mdl-34436065

RESUMO

Continuous microfluidic focusing of particles, both synthetic and biological, is significant for a wide range of applications in industry, biology and biomedicine. In this study, we demonstrate the focusing of particles in a microchannel embedded with glass grooves engraved by femtosecond pulse (fs) laser. Results showed that the laser-engraved microstructures were capable of directing polystyrene particles and mouse myoblast cells (C2C12) towards the center of the microchannel at low Reynolds numbers (Re < 1). Numerical simulation revealed that localized side-to-center secondary flows induced by grooves at the channel bottom play an essential role in particle lateral displacement. Additionally, the focusing performance proved to be dependent on the angle of grooves and the middle open space between the grooves based on both experiments and simulation. Particle sedimentation rate was found to critically influence the focusing of particles of different sizes. Taking advantage of the size-dependent particle lateral displacement, selective focusing of micrometer particles was demonstrated. This study systematically investigated continuous particle focusing in a groove-embedded microchannel. We expect that this device will be used for further applications, such as cell sensing and nanoparticle separation in biological and biomedical areas.

16.
Mol Med Rep ; 24(4)2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34396433

RESUMO

MicroRNA (miR)­4306 and FoxD2­adjacent opposite strand RNA 1 (FOXD2­AS1) are cancer­related genes involved in tumor progression. However, the potential functional roles of miR­4306 and FoxD2­AS1 in colorectal cancer (CRC) development remain unknown. The present study aimed to investigate the biological functions and the molecular mechanisms of miR­4306 and FoxD2­AS1 in CRC. Reverse transcription­quantitative PCR analysis was performed to determine the expression levels of FoxD2­AS1 and miR­4306 in CRC tissues and cell lines. Functional experiments, including Cell Counting Kit­8, colony formation, cell cycle assays and western blotting, were conducted to examine the effects of FoxD2­AS1 and miR­4306 on the malignant behaviors of CRC cells. In addition, the relationship between FoxD2­AS1 and miR­4306 was assessed using a dual­luciferase reporter assay and Pearson's correlation analysis. Compared with normal samples and cells, FoxD2­AS1 expression was increased and miR­4306 expression was decreased in CRC tissues and cells. Functional experiments demonstrated that silencing FoxD2­AS1 inhibited proliferation and induced cell arrest at G0/G1 phase in CRC cells, while the overexpression of FoxD2­AS1 showed opposite results. Ki­67 and proliferating cell nuclear antigen expression levels were decreased after transfection with small interfering RNA FoxD2­AS1, but were increased after transfection with FoxD2­AS1 overexpression plasmid. Furthermore, investigations into the underling mechanism revealed that FoxD2­AS1 functioned as a molecular sponge of miR­4306. The inhibitory effects of FoxD2­AS1 silencing on CRC progression were reversed by miR­4306 knockdown. Collectively, the present study demonstrated that FoxD2­AS1 functioned as an oncogene in CRC progression, and that miR­4306 could inhibit the malignant behaviors of CRC by regulating FoxD2­AS1. Thus, the current study provided a promising therapeutic target for CRC treatment.

17.
Genes (Basel) ; 12(6)2021 06 09.
Artigo em Inglês | MEDLINE | ID: mdl-34207778

RESUMO

microRNAs (miRNAs) play an important role in gene regulation in animals by pairing with target gene mRNA. Many miRNAs are differentially expressed in the adipose tissue, often with conserved expression. In our study, we found that miR-208b expression was observed differently in the preadipocyte differentiation model. When miR-208b was overexpressed in the preadipocyte differentiation model, the overexpressed group displayed higher expression of PPARγ and FABP4-the markers of preadipocyte differentiation. Oil Red O staining revealed that the count of lipid droplets was increased in the overexpressed group. When the expression of miR-208b was inhibited, the above indicators showed an opposite trend. Moreover, results from both 5-ethynyl-2'-deoxyuridine (EDU) and cell counting kit (CCK) analysis showed that miR-208b promoted the proliferation of preadipocyte. Expression of gene CSNK2A2, a direct miR-208b target, was downregulated in the overexpressed group, providing a possible link to multiple signal pathways. Overall, our data indicate that miR-208b play a positive regulatory effect on the proliferation and differentiation of rabbit preadipocyte.

18.
Se Pu ; 39(2): 197-202, 2021 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-34227352

RESUMO

Amisulpride is a clinically effective antipsychotic drug. It has been recommended for therapeutic drug monitoring in Consensus Guidelines for Therapeutic Drug Monitoring in Neuropsychopharmacology. An integrated multicolumn two-dimensional liquid chromatography system was constructed. Two reversed-phase columns, Supersil ODS2 (150 mm×4.6 mm, 5 µm) and SinoChrom ODS-BP (150 mm×4.6 mm, 5 µm), with different hydrophobicities were employed in the first and second separation dimensions, respectively. A strong cation-exchange short column, Supersil SCX (10 mm×4.6 mm, 5 µm) was used to trap samples after the first dimensional separation. A two-position six-port valve was applied to change the flow path for transferring the samples. An auxiliary pump was used to change the mobile phase between the first dimensional column and the trapping column. An intact method for analyzing amisulpride in serum was developed using an integrated multicolumn two-dimensional liquid chromatography system. Serum samples were pretreated only by protein precipitation and centrifugation. In the protein precipitation step, a mixture of perchloric acid (6%, v/v) and methanol was used as the precipitation reagent, whose volume was three times that of the serum sample. The use of this reagent helped eliminate the obvious solvent effects resulting from the large injection volume (300 µL). Then, the samples were vortexed for 2 min and centrifuged for 5 min at a velocity of 10000 r/min. The supernatant was injected into the system directly. Acetonitrile/phosphate buffer (25 mmol/L, pH 3.0; 20∶80, v/v) and acetonitrile/phosphate buffer (25 mmol/L, pH 7.0; 25∶75, v/v) were used as mobile phases for the first and second dimensions, respectively, at a flow rate of 1 mL/min. The solvent strength and pH of the first dimensional eluent were adjusted at the two-dimensional chromatographic interface. Phosphate buffer (25 mmol/L, pH 3.0) was supplied at a rate of 1 mL/min by the auxiliary pump for adjustment. The adjustment process allowed amisulpride to remain cationic, thus leading to improved transfer and trapping efficiencies in strong cation-exchange columns, in the heart-cutting mode. The trapping time was determined to be between 4 and 5 min by a confirmation experiment. The use of a short trapping column and the appropriate mobile phase conditions allowed us to complete the analysis within 12 min. The established method was validated in detail by investigating the linearity, limit of detection (LOD), limit of quantification (LOQ), and recovery. A good linear relationship was observed between 10 and 200 ng/mL (r=0.9998). The LOD and LOQ were 7.28 ng/mL and 24.27 ng/mL, respectively. The high sensitivity of the validated method met the requirements of the therapeutic reference range of the Consensus Guidelines for Therapeutic Drug Monitoring in Neuropsychopharmacology. The recovery of amisulpride spiked in a serum sample at 50 and 100 ng/mL were stabilized between 73.7% and 76.8%, which revealed the good stability of the established method. As opposed to the complicated traditional analytical methods, our method based on the automated integrated system is cost-effective and low-maintenance, thus being appropriate for routine therapeutic drug monitoring in clinical research. Moreover, our method is highly promising as the system cost is much lower than that of the popular LC-MS, while the capacity is sufficient for the determination of drugs in serum.


Assuntos
Amissulprida/sangue , Cromatografia Líquida , Humanos , Limite de Detecção
19.
Vet Med Sci ; 7(5): 2051-2060, 2021 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-34273256

RESUMO

Fatty acids of intramuscular fat (IMF) in rabbits can influence meat quality, but it is unclear which fatty acids benefit to human health. A rabbit model of weight gain and weight loss was constructed using two rabbit groups and two growth stages. Stage 1 included control group1 fed a commercial diet(CG1) and experimental group1 fed a high fat diet (EG1). Stage 2 include control group2(CG2) and experimental group2 (EG2) both fed a restricted commercial diet. We detected differences in blood biochemical indicators as well as changes in intramuscular adipose cells and intramuscular fatty acid content in control and experiment groups at two stages. High fat induction can make rabbits become obese, have higher concentrations of glucose (GLU), total cholesterol (TC), triglyceride (TG), low density lipoprotein-cholesterol (LDL-C) and free fatty acid (FFA), and lower concentrations of insulin (INS). In addition, a high-fat diet promotes hypertrophy of precursor adipocytes in femoral muscles. Conversely, a restricted diet causes weight loss, decreases the concentration of TG, FFA, and INS in CG2 and EG2, and increases the deposition of unsaturated fatty acids in the femoral muscle. The content of monounsaturated trans oleic acid (C18:1n-9T) in EG2 was significantly higher than in CG2, whereas oleic acid (C18:1n-9C) was significantly lower in EG2 than in CG2. The polyunsaturated fatty acids Linolenate (C18:3 n-3) and cis-5,8,11,14,17-Eicosapentaenoate (C20:5 n-3) increased in CG2 and EG2. The content of Linoleate (C18:2 n-6) and γ-Linolenic acid (C18:3 n-6) significantly increased in CG2. The content of cis-11,14-Eicosatrienoic acid (C20:2) decreased significantly in CG2, but increased significantly in EG2.Thus, a high-fat diet can increase the formation of unhealthy fatty acids. Conversely, weight loss due to a restricted diet leads to an increase in unsaturated fatty acids in the femoral muscle, indicating that it reduces obesity symptoms and it may improve meat quality in rabbit.

20.
Rev Sci Instrum ; 92(6): 065104, 2021 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-34243527

RESUMO

The internal high-speed geared rotor is the most important component of centrifugal compressors. The electrical runout error of the rotor including mechanical and electrical ones can greatly affect the working performances of the centrifugal compressors. To overcome this problem, this work proposes a practical comprehensive method for controlling the electrical runout of the rotor of a geared centrifugal compressor. An elastic-plastic deformation calculation method based on the slice approach is presented to determine the applied load range calculation for controlling the cross-sectional deviation of the rotor profile. In this method, the demagnetism and remanence detection should be provided to obtain a small residual magnetism value before the mechanical runout detection. A combined method including cold extrusion processing and slight impact processing is proposed to reduce the electrical runout of the rotor. A case study is carried out to validate the proposed method. To avoid the effect of the rotation precision and clearance of the support system on the detection results, a rigid support system is used to detect the mechanical runout of the rotor. Note that single cold extrusion or impact processing cannot completely eliminate the electrical runout. Finally, the results show that the proposed combined method can be a helpful approach in controlling the electrical runout of the rotors.

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