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1.
Nanoscale Res Lett ; 14(1): 181, 2019 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-31144185

RESUMO

The effect of nitridation treatment on the band alignment between few-layer MoS2 and HfO2 has been investigated by X-ray photoelectron spectroscopy. The valence (conduction) band offsets of MoS2/HfO2 with and without nitridation treatment were determined to be 2.09 ± 0.1 (2.41 ± 0.1) and 2.34 ± 0.1 (2.16 ± 0.1) eV, respectively. The tunable band alignment could be attributed to the Mo-N bonding formation and surface band bending for HfO2 triggered by nitridation. This study on the energy band engineering of MoS2/HfO2 heterojunctions may also be extended to other high-k dielectrics for integrating with two-dimensional materials to design and optimize their electronic devices.

2.
J Int Med Res ; 47(2): 859-866, 2019 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-30556437

RESUMO

OBJECTIVE: Intussusception is a common paediatric abdominal emergency in infants. The first-line treatment of choice in uncomplicated paediatric intussusception is enema reduction. The study aim was to provide an overview of the current national practice of enema reduction of paediatric intussusception in China. METHODS: A questionnaire on enema reduction of paediatric intussusception was sent to respondents (members of the Pediatric Anorectal Group, the Neonatal Group, the Society of Pediatric Surgery and the China Medical Association). RESULTS: Data from 128 questionnaires were analysed. Of these, 78.1% (100/128) reported the use of fluoroscopy, 17.2% (22/128) use of ultrasound monitoring, 78.9% (101/128) use of air and 17.9% (23/128) use of normal saline. A total of 78.9% (101/128) reported a success rate of 90%, 25.8% (33/128) reported that a paediatric surgeon managed the reduction, 18.8% (24/128) that a radiologist managed the reduction and 44.5% (57/128) that a paediatric surgeon and radiologist jointly managed the reduction. CONCLUSIONS: There is large variation in the techniques of enema reduction of intussusception in China. Fluoroscopy-guided air enema reduction is mainly used. Enema reduction of uncomplicated cases of paediatric intussusception in China lacks standardization of equipment and personnel involvement.


Assuntos
Enema/métodos , Intussuscepção/terapia , Guias de Prática Clínica como Assunto/normas , Padrões de Prática Médica/normas , Criança , China , Humanos , Prognóstico , Inquéritos e Questionários
3.
World J Clin Cases ; 6(14): 847-853, 2018 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-30510954

RESUMO

Heterotopic pancreas (HP) is a congenital anomaly defined as pancreatic tissue that has no contact with the orthotopic pancreas and its own duct system and vascular supply. The most common locations of HP are the upper gastrointestinal tract, specifically, the stomach, duodenum, and proximal jejunum. Involvement of the mesentery is rare. Here, we describe a rare case of mesenteric heterotopic pancreas (MHP) in a 12-year-old girl who presented with acute abdomen. The patient underwent emergency laparotomy, and the mass and adjacent small bowel were resected. Results of the postoperative histopathologic examination confirmed the diagnosis of MHP. Observation of the patient for 12 mo postoperatively showed no evidence of recurrence. Preoperative diagnosis of HP is difficult, even in a symptomatic patient. Increased awareness and understanding of the image characteristics of MHP will aid in correct preoperative diagnosis and appropriate patient management.

4.
Int J Mol Med ; 42(5): 2363-2372, 2018 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-30106085

RESUMO

Anorectal malformations (ARMs) are one of the most common congenital malformations of the digestive tract; however, the pathogenesis of this disease remains to be fully elucidated. MicroRNAs (miRNAs) are important in gastrointestinal development and may be involved in the pathogenesis of ARMs. The present study aimed to profile miRNAs and examine their potential functions in rats with ethylenethiourea (ETU)­induced ARMs. Pregnant Wistar rats (n=36) were divided randomly into ETU­treated and control groups. The rats in the ETU­treated group were gavage­fed 1% ETU (125 mg/kg) on gestational day 10 (GD10), whereas the control group rats received a corresponding dose of saline. Embryos were harvested by cesarean section on GD14, GD15 and GD16. Hindgut tissue was isolated from the fetuses for RNA extraction and microarray analysis, followed by bioinformatics analysis and reverse transcription­quantitative polymerase chain reaction (RT­qPCR) validation. Overall, 38 miRNAs were differentially expressed (all upregulated) on GD14, 49 (32 upregulated and 17 downregulated) on GD15, and 42 (all upregulated) on GD16 in the ARM group compared with the normal group. The top 18 miRNAs with |log2(fold change)| >4.25 were selected for further bioinformatics analysis. Among these miRNAs, five were differentially expressed at two time-points and were involved in ARM­associated signaling pathways. The RT­qPCR analysis revealed that three miRNA (miR), miR­125b­2­3p, miR­92a­2­5p and miR­99a­5p, were significantly differentially expressed in rats with ARMs compared with the normal group. In conclusion, the results suggested that the differential expression of miR­125b­2­3p, miR­92a­2­5p and miR­99a­5p during key time-points of anorectal formation in rats may have functions in the pathogenesis of ARM.


Assuntos
Malformações Anorretais/induzido quimicamente , Malformações Anorretais/genética , Embrião de Mamíferos/metabolismo , Etilenotioureia/toxicidade , MicroRNAs/metabolismo , Análise em Microsséries/métodos , Animais , Biologia Computacional , Feminino , Gravidez , Ratos , Ratos Wistar , Reação em Cadeia da Polimerase Via Transcriptase Reversa
5.
PeerJ ; 6: e4445, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29507836

RESUMO

Purpose: This study was performed to investigate the expression pattern of Wnt inhibitory factor 1 (Wif1) and ß-catenin during anorectal development in normal and anorectal malformation (ARM) embryos and the possible role of Wif1 and ß-catenin in the pathogenesis of ARM. Methods: ARM was induced with ethylenethiourea on the 10th gestational day in rat embryos. Cesarean deliveries were performed to harvest the embryos. The expression pattern of Wif1 and ß-catenin protein and mRNA was evaluated in normal rat embryos (n = 288) and ARM rat embryos (n = 306) from GD13 to GD16 using immunohistochemical staining, Western blot, and real time RT-PCR. Results: Immunohistochemical staining revealed that in normal embryos Wif1 was constantly expressed in the cloaca from GD13 to GD16. On GD13 and GD14, Wif1-immunopositive cells were extensively expressed in the cloaca. On GD15, the expression of Wif1 were mainly detected on the very thin anal membrane. In ARM embryos, the epithelium of the hindgut and urorectal septum demonstrated faint immunostaining for Wif1 from GD14 to GD16. Western blot and real time RT-PCR revealed that Wif1 and ß-catenin protein and mRNA expression level was significantly decreased in the ARM groups compared with the normal group on GD14 and GD15 (p < 0.05). Conclusions: This study demonstrated that the expression pattern of Wif1 and ß-catenin was disrupted in ARM embryos during anorectal morphogenesis, which demonstrated that downregulation of Wif1 and ß-catenin at the time of cloacal separation into the primitive rectum and urogenital septum might related to the development of ARM.

6.
J Mol Histol ; 48(5-6): 379-387, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-28975535

RESUMO

This study examined the expression patterns of proprotein convertase subtilisin/kexin type 5 (Pcsk5) during anorectal development in normal and anorectal malformations (ARM) rat embryos, determine the possible role of Pcsk5 in the pathogenesis of ARM. An ARM rat model was developed by the administration of ethylenethiourea gestational day 10 (GD10). Embryos were harvested by surgical excision from GD13 to GD16, and the spatiotemporal expression of Pcsk5 was evaluated, using immunohistochemistry staining, Western blotting and real time RT-PCR. Immunohistochemistry staining in normal embryos revealed that Pcsk5 was abundantly expressed on the epithelium of the cloaca (CL) on GD13. On GD14 and GD15, positive cells were noted on the urorectal septum and the thin anal membrane. However, the epithelium of the CL of ARM embryos only faintly expressed Pcsk5 from GD13 to GD15. Western blotting and real time RT-PCR showed time-dependent increase of Pcsk5 expression in the developing hindgut. Pcsk5 expression levels were lower in the ARM group from GD14 to GD16 (p ≤ 0.05). These results indicate that downregulation of Pcsk5 during cloaca development into the rectum and urethra might be related to the formation of ARMs.


Assuntos
Malformações Anorretais/genética , Feto/anormalidades , Feto/metabolismo , Pró-Proteína Convertase 5/genética , Animais , Malformações Anorretais/patologia , Western Blotting , Cloaca/embriologia , Embrião de Mamíferos/anormalidades , Embrião de Mamíferos/metabolismo , Imuno-Histoquímica , Pró-Proteína Convertase 5/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos Wistar , Reação em Cadeia da Polimerase em Tempo Real
7.
J Xray Sci Technol ; 25(2): 193-204, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28234272

RESUMO

Real-time monitoring and amendment of patient position is important for the radiotherapy. However, using electronic portal imaging device (EPID) and cone beam computer tomography (CBCT) in the clinical practice generate different degrees of delay, so that they cannot achieve the purpose of real-time application. Meanwhile, a few products come with the function of the real-time monitoring and amendment, such as CyberKnife, which is too expensive for the common people. The objective of this study is to develop and test a novel independent system to monitor treatment center and amend the position of patient, which is applicable to most accelerators, based on binocular location. The system monitors the treatment center by tracking the markers attached to the patient. Once the treatment center shifts, the system uses the magic finger, which is developed to control the treatment bet automatically to adjust the treatment bed position. To improve the monitoring accuracy, we trained the data collected from the clinic based on SVM (Support Vector Machine). Thus, the training results assist users to adjust the feasible degree of the monitoring. The experiment results showed that using this new monitoring system, the monitoring resolution reached 0.5 mm, and the error ratio of the judgment was less than 1.5%.


Assuntos
Processamento de Imagem Assistida por Computador/métodos , Posicionamento do Paciente/métodos , Radioterapia Assistida por Computador/métodos , Humanos , Máquina de Vetores de Suporte , Tomografia Computadorizada por Raios X
8.
Exp Mol Pathol ; 101(1): 143-9, 2016 08.
Artigo em Inglês | MEDLINE | ID: mdl-27477499

RESUMO

The aim of this study was to determine Bmpr1a and Bmp2 expression patterns during anorectal development in normal and anorectal malformation (ARM) embryos with a view to establishing the possible role of Bmpr1a and Bmp2 in ARM pathogenesis. ARM was induced with ethylenethiourea on the 10th gestational day (GD10) in rat embryos. The embryos were harvested by Cesarean deliveries. The expression of Bmpr1a and Bmp2 was evaluated in normal rat embryos (n=213) and ARM embryos (n=236) from GD14 to GD16. Immunohistochemical staining revealed, in normal embryos, that Bmpr1a and Bmp2 was mainly expressed on the epithelium of the urorectal septum (URS) and the cloacal membrane (CM) on GD14 and GD15. When the rectum separated from the urogenital sinus (UGS) on GD16, Bmpr1a- and Bmp2-immunolabeled cells were observed on the anorectal epithelium. In ARM embryos, the epithelium of the hindgut and URS demonstrated faint immunostaining for Bmpr1a and Bmp2. Analyses by Western blot and Real-time PCR revealed that Bmpr1a and Bmp2 protein and mRNA expression were significantly decreased in the ARM hindgut compared with normal hindgut on GD14 and GD15 (P<0.05). In ARM embryos, an imbalance in the spatiotemporal expression of Bmpr1a and Bmp2 was noted during anorectal morphogenesis from GD14 to GD16. Therefore, downregulation of Bmpr1a and Bmp2 at the time of cloacal separation into the primitive rectum and UGS might be related to the development of ARM.


Assuntos
Malformações Anorretais/genética , Proteína Morfogenética Óssea 2/genética , Receptores de Proteínas Morfogenéticas Ósseas Tipo I/genética , Sistema Digestório/embriologia , Embrião de Mamíferos/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Animais , Malformações Anorretais/patologia , Western Blotting , Proteína Morfogenética Óssea 2/metabolismo , Receptores de Proteínas Morfogenéticas Ósseas Tipo I/metabolismo , Sistema Digestório/metabolismo , Idade Gestacional , Imuno-Histoquímica , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Ratos Wistar , Reação em Cadeia da Polimerase em Tempo Real
9.
PeerJ ; 4: e1771, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-27042391

RESUMO

Background. The objectives of this study were to determine the spatiotemporal distribution of human caudal-type homeobox proteins CDX1, CDX2 and CDX4 during development of the hindgut and anorectum in the embryo and to explore the possible roles of CDX genes during morphogenesis of the hindgut and anorectum. Methods. Embryos (89) were cut into sections serially and sagittally. From gestation weeks 4-9, CDX1, CDX2 and CDX4 proteins were detected on the caudal midline by immunohistochemical staining. Results. During week 4, extensive immunoreactivity of CDX1, CDX2 and CDX4 was detected in the dorsal urorectal septum, urogenital sinus and hindgut. From weeks 5-7, CDX1-, CDX2- and CDX4- positive cells were detected mainly in the mesenchyme of the urorectal septum and hindgut. The levels of CDX2 and CDX4 immunoreactivity were lower compared to CDX1. During weeks 8 and 9, the anorectal epithelium stained positive for CDX1 and CDX4, and the anal epithelium was positive for CDX2. Conclusions. The CDX proteins are constantly distributed during development of the hindgut and anorectum and exhibit overlapping distribution patterns in the cloaca/hindgut, suggesting they are important in the morphogenesis of the human hindgut and anorectum. CDX genes might be involved in development of the anorectal epithelium after the rectum has separated from the urorectal septum.

10.
Clin Chim Acta ; 450: 349-55, 2015 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-26368265

RESUMO

BACKGROUND: Angiogenesis is a fundamental process for brain development and repair. Thrombospondin-1 is the first identified endogenous angiogenesis inhibitor. Its expression in rat brain is upregulated after intracerebral hemorrhage (ICH). We determined whether plasma thrombospondin-1 concentrations are associated with injury severity and prognosis in ICH patients. METHODS: This observational, prospective study recruited 110 patients and 110 age- and gender-matched healthy controls. Blood samples were collected from the patients at admission and from the healthy controls at study entry to measure plasma thrombospondin-1 concentrations. The endpoints included 1-week mortality, 6-month mortality, 6-month overall survival and 6-month unfavorable outcome (modified Rankin Scale score >2). RESULTS: Plasma thrombospondin-1 concentrations were markedly higher in patients than in healthy controls. Thrombospondin-1 was an independent predictive factor for all endpoints and plasma thrombospondin-1 concentrations were highly associated with injury severity reflected by hematoma volume and National Institutes of Health Stroke Scale score. Under receiver operating characteristic curves, plasma thrombospondin-1 concentrations had similar predictive values compared with hematoma volume and National Institutes of Health Stroke Scale score. CONCLUSIONS: Increased plasma thrombospondin-1 concentrations following ICH are independently associated with injury severity and short-term and long-term clinical outcomes.


Assuntos
Hemorragia Cerebral/sangue , Trombospondina 1/sangue , Doença Aguda , Adulto , Idoso , Hemorragia Cerebral/diagnóstico , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Prognóstico , Análise de Sobrevida
11.
Int J Clin Exp Pathol ; 8(4): 3727-34, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26097554

RESUMO

The aim of this study was to determine the expression patterns of bone morphogenetic protein 7 (BMP7) during anorectal development in normal rat embryos and in embryos with anorectal malformations (ARM), and to investigate the possible role of BMP7 in the pathogenesis of ARM. ARM was induced by treating rat embryos with ethylenethiourea on the 10th gestational day (GD10). Embryos were harvested by Cesarean delivery and the spatiotemporal expression of BMP7 was evaluated in normal (n=168) and ARM embryos (n=171) from GD13 to GD16 using immunohistochemistry staining and western blot analysis. Immunohistochemical staining in normal embryos revealed that BMP7 was abundantly expressed on the epithelium of the urorectal septum (URS) and the hindgut on GD13, and BMP7-immunopositive cells were extensively detected in the URS, hindgut, and cloacal membrane by GD14. Increased positive tissue staining was noted on the fused tissue of the URS and the thin anal membrane on GD15. In ARM embryos, the epithelium of the cloaca, URS, and anorectum were negatively or only faintly immunostained for BMP7. BMP7 protein expression showed time-dependent changes in the developing hindgut according to western blotting, and reached a peak on GD15 during anus formation. BMP7 expression levels from GD14 to GD15 were significantly lower in the ARM group compared with the normal group (P<0.05). Spatiotemporal expression of BMP7 was disrupted in ARM embryos during anorectal morphogenesis from GD13 to GD16. These results suggest that downregulation of BMP7 at the time of cloacal separation into the primitive rectum and UGS might be related to the development of ARM.


Assuntos
Canal Anal/anormalidades , Anus Imperfurado/metabolismo , Proteína Morfogenética Óssea 7/metabolismo , Reto/anormalidades , Canal Anal/metabolismo , Animais , Malformações Anorretais , Anus Imperfurado/induzido quimicamente , Regulação para Baixo , Epitélio/metabolismo , Etilenotioureia , Feminino , Gravidez , Ratos , Ratos Wistar , Reto/metabolismo
12.
Int J Clin Exp Med ; 8(11): 19895-904, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26884902

RESUMO

BACKGROUND: This study aimed to determine the spatiotemporal expression of caudal-type homeobox genes (CDX1, CDX2 and CDX4) during development of the midgut in human embryos and to explore the possible roles of CDX genes during the morphogenesis of human midgut. Human embryos (n=28) were sectioned serially and sagittally and CDX1, CDX2 and CDX4 proteins were detected on the midline from the 5th to 9th weeks of gestation by immunohistochemical staining. RESULTS: CDX1, CDX2 and CDX4 proteins were weakly expressed in epithelium and mesenchyme of the midgut in the 6th and 7th weeks of gestation and reached estimated optimal level on the 8th and 9th weeks of gestation. In the 9th week of gestation, immunoreactivities specific to CDX1, CDX2 and CDX4 were restricted in epithelium of the midgut. CONCLUSIONS: CDX1, CDX2 and CDX4 proteins began to express in human midgut in the 6th week of gestation. From the 6th to 9th week of gastation, the expression of CDX1, CDX2 and CDX4 proteins gradually increase and exhibited overlapping expression patterns, suggesting that CDX genes may be involved in early development of the epithelium of human midgut. Cross-regulatory interactions may exist among CDX genes with respect to human midgut development.

13.
Cells Tissues Organs ; 199(2-3): 212-20, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25401498

RESUMO

PURPOSE: The aim of this study was to determine the expression of Cdx4 (caudal-type homeobox gene-4) during anorectal development in normal and ethylenethiourea (ETU)-induced anorectal malformation (ARM) embryos with a view to establishing the possible role of Cdx4 in ARM pathogenesis. MATERIALS AND METHODS: ARM was induced by ETU on the 10th gestational day (GD10) in rat embryos. Cesarean deliveries were then performed to harvest the embryos. Spatiotemporal expression of Cdx4 was evaluated in normal rat embryos (n = 354) and ARM embryos (n = 378) from GD13 to GD16. RESULTS: Immunohistochemical staining and immunofluorescence revealed that, in normal embryos, Cdx4 expression was extensively detected on the epithelium of the cloaca on GD13. On GD14, the Cdx4-positive cells were intensively detected on the hindgut. On GD15, the anal membrane was constantly immunoreactive to Cdx4. On GD16, Cdx4-labeled cells were observed on the epithelium of the anus. In the ARM embryos, the epithelium of the cloaca, urorectal septum (URS) and anorectum was negative or faint for Cdx4. In the normal embryo group, Cdx4 protein and mRNA expression showed time-dependent changes in the developing hindgut from GD13 to GD16 on Western blot and real-time reverse transcription plus polymerase chain reaction. Once the URS divided the cloaca into the primitive rectum and urogenital sinus (UGS) on GD15, Cdx4 expression began to decrease. In addition, the expression level of Cdx4 in the ARM group from GD13 to GD15 was significantly lower than that in the normal group (p < 0.05). CONCLUSIONS: In ARM embryos, an imbalance in the spatiotemporal expression of Cdx4 was noted during anorectal morphogenesis from GD13 to GD16. This suggests that ETU may cause downregulation of Cdx4 expression. Downregulation of Cdx4 at the time of cloacal separation into the primitive rectum and UGS might thus be related to the development of ARM.


Assuntos
Canal Anal/metabolismo , Anus Imperfurado/embriologia , Anus Imperfurado/metabolismo , Etilenotioureia/toxicidade , Proteínas de Homeodomínio/metabolismo , Reto/metabolismo , Canal Anal/anormalidades , Canal Anal/efeitos dos fármacos , Animais , Malformações Anorretais , Anus Imperfurado/induzido quimicamente , Feminino , Regulação da Expressão Gênica no Desenvolvimento/efeitos dos fármacos , Imuno-Histoquímica , Masculino , Morfogênese/genética , Morfogênese/fisiologia , Gravidez , Ratos , Reto/anormalidades , Reto/efeitos dos fármacos
14.
Cell Tissue Res ; 357(1): 83-90, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24744267

RESUMO

The main aim of this study was to determine Cdx2 expression patterns during anorectal development in normal and anorectal malformation (ARM) embryos with a view to establishing the possible role of Cdx2 in ARM pathogenesis. ARM was induced with ethylenethiourea on the 10th gestational day (GD10) in rat embryos, and Cesarean deliveries were performed to harvest the embryos. The temporal and spatial expression of Cdx2 was evaluated in normal rat embryos (n = 303) and ARM embryos (n = 321) from GD13 to GD16. Immunohistochemical staining revealed that, in normal embryos, Cdx2 was mainly expressed on the epithelium of the urorectal septum (URS) and the hindgut on GD13. On GD14, Cdx2-immunopositive cells were extensively detected on the URS, hindgut, and cloacal membrane. On GD15, increased immunopositive tissue staining on the anal membrane was evident. In ARM embryos, the epithelium of the cloaca, URS, and anorectum were negative or faintly immunostaining for Cdx2. Analyses by Western blot and real-time reverse transcription plus the polymerase chain reaction revealed that, in the normal group, Cdx2 protein and mRNA expression showed time-dependent changes in the developing hindgut from GD13 to GD16. Upon the URS division of the cloaca into the primitive rectum and urogenital sinus (UGS) on GD15, Cdx2 expression began to decrease. Moreover, the Cdx2 expression level in the ARM group from GD13 to GD14 was significantly lower than that in the normal group (P < 0.05). In ARM embryos, an imbalance in the spatiotemporal expression of Cdx2 was noted during anorectal morphogenesis from GD13 to GD16. Downregulation of Cdx2 at the time of cloacal separation into the primitive rectum and UGS might thus be related to the development of ARM.


Assuntos
Canal Anal/anormalidades , Anus Imperfurado/metabolismo , Proteínas de Homeodomínio/biossíntese , Fatores de Transcrição/biossíntese , Canal Anal/efeitos dos fármacos , Canal Anal/embriologia , Animais , Malformações Anorretais , Anus Imperfurado/induzido quimicamente , Anus Imperfurado/genética , Fator de Transcrição CDX2 , Modelos Animais de Doenças , Etilenotioureia , Feminino , Proteínas de Homeodomínio/genética , Masculino , Morfogênese/genética , Gravidez , Ratos , Ratos Wistar , Fatores de Transcrição/genética
15.
Cells Tissues Organs ; 198(1): 28-34, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23774963

RESUMO

As fibroblast growth factor 10 (FGF-10) gene expression may have a role in anorectal duct formation, this study aimed to assess the spatiotemporal expression pattern of FGF-10 during development of the rectum and hindgut in human embryos. FGF-10 expression was evaluated in human embryos (n = 85) at 3-8 weeks of gestation after immunohistochemical evaluation using antibodies specific for FGF-10. From weeks 4 to 7 of gestation, FGF-10 expression was observed primarily in the apical epithelium of the dorsal urorectal septum, the cloacal membrane (CM) and the hindgut. Following CM rupture (week 7), the epithelium of the anal canal was negative for FGF-10; however, it was present within the urothelium through week 7. FGF-10 expression during the development of the human hindgut and anorectum suggests that it may play a role in hindgut and anorectal morphogenesis.


Assuntos
Canal Anal/embriologia , Fator 10 de Crescimento de Fibroblastos/análise , Reto/embriologia , Canal Anal/metabolismo , Canal Anal/ultraestrutura , Feminino , Fator 10 de Crescimento de Fibroblastos/genética , Regulação da Expressão Gênica no Desenvolvimento , Humanos , Imuno-Histoquímica , Gravidez , Reto/metabolismo , Reto/ultraestrutura
16.
Int J Med Sci ; 10(2): 191-7, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23329892

RESUMO

BACKGROUND: Anorectal malformations (ARMs) represent a variety of congenital disorders that involve abnormal termination of the anorectum. This study was to reveal relation between CDX1 and human ARMs phenotypes. METHODS: 108 Chinese patients and 120 Chinese controls were included in this study. We analyzed the relation between two by PCR, qRT-PCR, western blot and immunofluorescence. RESULTS: Four heterozygous mutations in CDX1 gene were identified in ARMs patients (3.7%, 4/108), no found in controls. CDX1 protein expression was significantly decreased in the ARMs compared with the control anorectum. All samples analyzed in ARMs group exhibited down-regulated CDX1 mRNA expression in comparison to matched normal group, demonstrated significant differences statistically. CONCLUSION: The findings represented the relation between CDX1 mutations and CDX1 genotype. Furthermore, it was suggested that the downregulation of CDX1 might be related to the development of ARMs.


Assuntos
Anus Imperfurado , Proteínas de Homeodomínio/genética , Malformações Anorretais , Anus Imperfurado/genética , Anus Imperfurado/fisiopatologia , Regulação para Baixo , Feminino , Proteínas de Homeodomínio/metabolismo , Humanos , Lactente , Recém-Nascido , Masculino , Mutação , Reto/anormalidades
17.
J Pediatr Surg ; 46(1): 233-6, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21238675

RESUMO

Stump appendicitis is a delayed complication of incomplete appendectomy. Reinflammation of possible residual appendiceal tissue should be considered in patients with right lower quadrant pain and a surgical history of appendectomy. In this report, we present 3 cases of stump appendicitis in children.


Assuntos
Apendicectomia , Apendicite/diagnóstico , Apendicite/cirurgia , Complicações Pós-Operatórias/diagnóstico , Complicações Pós-Operatórias/cirurgia , Dor Abdominal/diagnóstico , Dor Abdominal/diagnóstico por imagem , Adolescente , Apendicectomia/efeitos adversos , Apendicite/patologia , Apêndice/patologia , Apêndice/cirurgia , Criança , Feminino , Humanos , Laparoscopia , Masculino , Complicações Pós-Operatórias/patologia , Reoperação , Tomografia Computadorizada por Raios X
18.
Int J Colorectal Dis ; 26(1): 109-16, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-20686776

RESUMO

PURPOSE: The aim of the present analysis is to examine the morphological changes, the spatiotemporal distribution of apoptosis/proliferation in the human embryonic anorectum, to reveal the normal development of human anorectum, and investigate the possible roles of apoptosis/proliferation during anorectal development. MATERIALS AND METHODS: The embryos were sectioned serially and sagittally, stained with hematoxylin and eosin (H & E) between the third and eighth week of gestation, TdT-mediated dUTP-digoxigenin nick end-labeling (TUNEL) and proliferative cell-specific nuclear antigen (PCNA) immunohistochemical staining from the sixth to the eighth week. RESULTS: From the fourth to the seventh week, with the growth of the mesenchyme around the cloaca, the cloaca was remolded, subsequently, the cloacal membrane (CM) moved perpendicularly then horizontally. The dorsal cloaca gradually descended to the tail groove, the urorectal septum (URS) and the CM approximated; however, the fusion of URS with the dorsal CM was never observed. During the eighth week, the URS shifted ventrally and finally fused with the ventral CM. Moreover, from the sixth to the eighth week, the apoptotic cells were concentrated in the CM, the mesenchyme of terminal rectum, and the dorsal rectum. Meanwhile, the proliferative cells could be observed in the ventral mesenchyme around the cloaca, the CM, the fused tissue between the URS, and the ventral CM. CONCLUSIONS: During the development of human anorectum, it was intriguing to reveal that the URS never fused with the dorsal CM before dorsal CM disintegration, the normal anorectal development may depend on the dorsal cloaca and the dorsal CM; furthermore, the distribution of apoptosis and proliferation in the anorectum and ventral cloacal mesenchyme played a pivotal role in the formation of the anorectum.


Assuntos
Canal Anal/embriologia , Embrião de Mamíferos/anatomia & histologia , Embrião de Mamíferos/embriologia , Reto/embriologia , Canal Anal/citologia , Apoptose , Proliferação de Células , Embrião de Mamíferos/citologia , Humanos , Antígeno Nuclear de Célula em Proliferação/metabolismo , Reto/citologia , Fatores de Tempo
19.
J Pediatr Surg ; 44(4): 859-61, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19361656

RESUMO

Lipoblastoma is a rare benign neoplasm of embryonal fat that occurs typically in infancy and childhood. An unusual case of a giant mesenteric lipoblastoma in a 4-year-old child is presented. The lipoblastoma was completely removed. Histopathologic examination confirmed the diagnosis of mesenteric lipoblastoma. Observation of the patient for 4 months postoperatively showed no evidence of recurrence.


Assuntos
Lipoma/patologia , Mesentério/patologia , Neoplasias Peritoneais/patologia , Dor Abdominal/diagnóstico , Dor Abdominal/etiologia , Biópsia por Agulha , Pré-Escolar , Seguimentos , Humanos , Imuno-Histoquímica , Laparotomia/métodos , Lipoma/diagnóstico , Lipoma/cirurgia , Imagem por Ressonância Magnética , Masculino , Estadiamento de Neoplasias , Neoplasias Peritoneais/diagnóstico , Neoplasias Peritoneais/cirurgia , Medição de Risco , Tomografia Computadorizada por Raios X , Resultado do Tratamento
20.
J Pediatr Surg ; 43(12): 2305-7, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19040961

RESUMO

An intraluminal pyloric duplication cyst is an extremely rare congenital anomaly. We report a case of an intraluminal pyloric duplication cyst in a 2-year-old girl with progressive increased vomiting. The patient had an ultrasonography, upper gastrointestinal series, and multiplanar reformatted computer tomography of the abdomen. The diagnosis was confirmed by surgery and histopathologic examination. The patient was asymptomatic at the 12-month follow-up.


Assuntos
Cistos/congênito , Obstrução da Saída Gástrica/congênito , Piloro/anormalidades , Gastropatias/congênito , Pré-Escolar , Cistos/complicações , Cistos/diagnóstico por imagem , Cistos/cirurgia , Feminino , Obstrução da Saída Gástrica/etiologia , Obstrução da Saída Gástrica/cirurgia , Humanos , Laparotomia , Piloro/diagnóstico por imagem , Piloro/cirurgia , Gastropatias/complicações , Gastropatias/diagnóstico por imagem , Gastropatias/cirurgia , Tomografia Computadorizada por Raios X , Ultrassonografia , Vômito/etiologia
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