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1.
Gynecol Obstet Invest ; 85(1): 53-71, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31505492

RESUMO

OBJECT: The purpose of this review is to assess the diagnostic performance of different imaging techniques for the detection of para-aortic lymph node (PALN) metastasis from gynecological malignancies. METHODS: Six databases, from the earliest available date of indexing through July 22, 2018, were systematically searched. In addition, the reference lists of relevant articles were searched by hand. Study allocation, data extraction, and quality assessment were independently performed by 2 reviewers. The size effect, sensitivity (SEN), specificity (SPE), positive likelihood ratio, negative likelihood ratio, diagnostic OR, and 95% CIs were used in the meta-analysis. The area under the curve (AUC) and Q* were calculated to reflect the synthesized diagnostic accuracy. Statistical calculations of this meta-analysis were conducted using STATA version 14.0 software. RESULTS: Across 41 eligible studies (1,615 participants), pooled SEN, SPE, and AUC of magnetic resonance imaging (MRI), computed tomography (CT), positron emission tomography (PET), PET-CT, and lymphangiography analyses were 25%, 93%, 0.7675; 60%, 94%, 0.9050; 83%, 96%, 0.9422; 66%, 97%, 0.9501; 77%, 75%, 0.8332, respectively. Analysis of combined summary receiver operating characteristic curves indicated that PET and PET-CT were superior to other imaging modalities. CONCLUSION: The present meta-analysis demonstrated that PET and PET-CT should be the first choice for detecting PALN metastasis in gynecological malignancies. CT was also suitable for confirmation. MRI was not recommended. Further studies are needed for PALN assessment.

2.
J Cancer ; 10(23): 5770-5784, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31737114

RESUMO

OSCC (oral squamous carcinoma) is one of most common malignant cancer. Although previous studies have found abnormal expression of B7-H3 in human OSCC, the exact role and molecular mechanism of B7-H3 in OSCC remain unknown. In this study, we investigated the role of B7-H3 in glucose metabolic reprogramming of OSCC cells in vitro and in vivo. We first detected the expression of B7-H3 in OSCC samples. Next, siRNAs and overexpression short-hairpin RNA of B7-H3 were transfected into SCC25 and Cal27 cells, and cell proliferation, migration and invasion were analyzed via CCK8, colony formation and transwell assays. Then glycolysis flux was determined through measuring glucose uptake and lactate production, and mRNA and protein expression levels were determined by real-time quantitative PCR and western blot respectively. The results presented here showed B7-H3 was upregulated in OSCC samples compared with normal tissues, and the expression level was associated with tumor size and nodal metastasis. B7-H3 affects OSCC cell proliferation, migration and invasion. We also found that B7-H3 promoted the Warburg effect, evidenced by increase glucose uptake and lactate production. We further demonstrated that B7-H3 enhanced OSCC glycolysis through the upregulation of HIF-1α and its downstream targets, Glut1 and PFKFB3, which are key factors in glycolysis. Mechanically, we demonstrated that B7-H3 regulates HIF-1α expression through PI3K/Akt/mTOR pathway. Metabolic imaging of human OSCC cancer xenograft in mice confirmed that B7-H3 enhanced tumor glucose uptake, glycolysis promoted genes expression and tumor growth. Taken together, our results have unveiled a mechanism that B7-H3 drives OSCC progression through enhancing of glycolytic metabolic program in OSCC.

3.
J Dent Sci ; 13(4): 342-349, 2018 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-30895143

RESUMO

Background/purpose: The surgical removal of mandibular third molars is frequently accompanied by significant postsurgical sequelae. Different instruments such as piezosurgery and conventional rotary handpiece have been used to decrease such adverse events. There are controversial results from randomized controlled trials evaluating the effects of Piezosurgery in the mandibular third molar extraction, compared with conventional rotary instrument. This study was performed to determine the impact of piezosurgery versus conventional rotary instrument on postoperative reactions after extraction. Materials and methods: A systematic review and meta-analysis was performed to combine relevant RCTs results. Results: Five RCTs were eligible for this study, enrolling a total of 402 patients. Compared with conventional rotary instrument, pain score at 6 or 7 days and mouth opening at 1 day after surgery was significantly lower in Piezosurgery group (SMD -0.33, 95% CI: -0.56 to -0.10, P = 0.005), as well as swelling score at 7 days after surgery (SMD -1.95, 95% CI: -3.22 to -0.67, P = 0.003). Furthermore, mouth opening at 1 day after surgery was significantly better in patients treated with Piezosurgery (SMD 0.84, 95% CI: 0.19 to 1.49, P = 0.01). However, more operation time will be required for Piezosurgery (MD 6.23, 95% CI: 3.32 to 9.14, P < 0.0001). With regard to analgesic dosage, pooled results from two RCTs suggested there were no significant differences between Piezosurgery and conventional rotary instrument (SMD -1.45, 95% CI: -4.39 to 1.49, P = 0.33). Conclusion: There might be some advantages on third mandibular molar extraction with piezosurgery compared to conventional rotary instrument. More multi-centre trials are required to get more conclusive results.

4.
Oncol Lett ; 14(6): 7705-7714, 2017 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-29250172

RESUMO

The present study aimed to observe the effect of the biological functions of integrin-linked kinase (ILK) silencing combined with hyperthermia on Tca8113 cells. Lentivirus-mediated short hairpin RNA (shRNA)-targeting ILK was transfected into oral squamous cell carcinoma (OSCC) Tca8113 cells and, combined with hyperthermia, several experimental methods were used to detect their biological behavior in vitro. On the basis of in vitro experiments, Tca8113 cells were transplanted into nude mice models, and ILK-shRNA-lentivirus was injected into the nude mice transplanted tumor and combined with hyperthermia. Tumor morphology and the associated protein expression changes were determined. Subsequent to ILK silencing combined with hyperthermia, the growth, migration and proliferation of Tca8113 cells were significantly inhibited. Flow cytometry revealed that the cells were blocked in the S phase, and western blot analysis demonstrated that ILK, phosphorylated (p)-RAC-alpha serine/threonine-protein kinase (Akt), p-glycogen synthase kinase-3ß and p-heat shock factor 1 protein expression levels were significantly decreased, while apoptosis-associated protein B-cell lymphoma-2-associated X protein expression and the efficacy of hypothermia were significantly increased. By ILK silencing combined with hyperthermia, a significant therapeutic effect on transplanted tumors was observed in nude mice. Immunohistochemistry revealed the same results as the in vitro experiments. ILK silencing combined with hyperthermia can inhibit the growth, proliferation and migration of Tca8113 cells, promote Tca8113 cell apoptosis, inhibit the phosphatidylinositol-3-kinase/Akt signaling pathway and increase hyperthermia sensitivity; the combination therapy exhibits a synergistic sensitizing effect. Therefore, ILK silencing combined with hypothermia may serve as a novel combination therapy strategy against OSCC.

5.
Med Sci Monit ; 23: 528-534, 2017 Jan 29.
Artigo em Inglês | MEDLINE | ID: mdl-28132066

RESUMO

BACKGROUND The nasopalatine nerve may be injured during extraction of teeth embedded in the anterior hard palate. The neural recovery process and its impact on sensation in the anterior hard palatal region are controversial. In our clinical practice, we noticed a distinct recovery process in children compared with adolescents or adults after surgery. We hypothesized that the sensory innervations of the anterior palate might shift during later childhood and pre-adolescence, which is due to the development of the nasopalatine nerve along with the maxillary growth and permanent teeth eruption. MATERIAL AND METHODS Forty patients (20 females and 20 males, mean age 11.8±2.2) with impacted supernumerary teeth in anterior palatine area were included into our study, and were divided into 3 groups according to their age. A 24-week follow-up was conducted and the sensation in the anterior hard palate region was examined at every check point. All the data were collected and analyzed by Kaplan-Meier analysis. RESULTS Fourteen children did not complain of any numbness immediately after anesthetization, and other children with sensory disorders had shorter healing periods compared to adolescent/adult patients. CONCLUSIONS The results indicated that the dominant nerve of the anterior hard palate region was dramatically changed from the greater palatine nerve to the nasopalatine nerve, which is important in deciding when to operate and in selection of anesthesia method.


Assuntos
Palato Duro/inervação , Transtornos das Sensações/etiologia , Adolescente , Fatores Etários , Criança , Feminino , Humanos , Estimativa de Kaplan-Meier , Masculino , Maxila/cirurgia , Palato Duro/cirurgia , Projetos Piloto , Transtornos das Sensações/metabolismo , Extração Dentária/efeitos adversos , Extração Dentária/métodos , Dente Impactado/cirurgia , Dente Supranumerário/cirurgia
6.
Oncol Rep ; 35(1): 89-98, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-26531674

RESUMO

Integrin-linked kinase (ILK), a highly conserved intracellular protein of serine/threonine protein kinase activities, which is associated with the integrin and growth factor receptor signaling pathway, is involved in the regulation of cell proliferation, apoptosis, differentiation, migration and epithelial-mesenchymal transition (EMT). Findings of a previous study showed that ILK overexpression was strongly correlated with a more aggressive tumor phenotype, recurrence and poor survival for oral squamous cell carcinoma (OSCC) patients, as well as some EMT markers. In order to investigate the underlying mechanisms involved, a lentivirus-mediated short hairpin RNA (shRNA) was employed to downregulate ILK. The results showed that the knockdown of ILK inhibited cell growth, adhesion and invasion ability in vitro, and OSCC cells deficient of ILK were blocked in the S phase and underwent apoptosis. Additionally, ILK shRNA inhibited EMT by impairing the expression of Snail, Slug and Twist2 and enhacning E-cadherin expression. ILK shRNA suppressed the phosphorylation of downstream signaling targets Akt and GSk-3ß. In addition, the knockdown of ILK inhibited tumor growth, invasion and metastasis of xenograft tumors in vivo. These results suggested that ILK is a promising therapeutic target for the treatment of OSCC.


Assuntos
Carcinoma de Células Escamosas/metabolismo , Carcinoma de Células Escamosas/terapia , Neoplasias Bucais/metabolismo , Neoplasias Bucais/terapia , Proteínas Serina-Treonina Quinases/antagonistas & inibidores , RNA Interferente Pequeno/genética , Carcinoma de Células Escamosas/genética , Adesão Celular , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Transição Epitelial-Mesenquimal , Regulação Neoplásica da Expressão Gênica , Vetores Genéticos/administração & dosagem , Humanos , Técnicas In Vitro , Lentivirus/genética , Neoplasias Bucais/genética , Transplante de Neoplasias , Ensaios Antitumorais Modelo de Xenoenxerto
7.
J Tissue Eng Regen Med ; 9(12): 1404-16, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-23365046

RESUMO

Vascularization is thought to be a principle obstacle in the reconstruction of skeletal muscle defects. Long-term survival of reconstructed skeletal muscle is dependent on good vascularization. In this study, we upregulated angiogenic gene expression in myoblasts in an attempt to promote vascularization during repair of skeletal muscle defects. Skeletal myoblasts were isolated and expanded from newborn male Sprague-Dawley (SD) rats. The cells were transfected with human vascular endothelial growth factor 165 (VEGF-165) or human stromal cell-derived factor 1 (SDF-1), using Lipofectamine™ 2000 transfection reagent, prior to seeding onto calf collagen scaffolds. Gene and protein overexpression was verified by ELISA, RT-PCR and western blot analysis. Cell-seeded scaffolds were transplanted into back muscle defects in female SD rats. At weeks 2, 4 and 8 after transplantation, Y chromosome detection was used to observe the survival of growth factor-producing cells within the scaffolds in vivo. Capillary density was investigated using microvessel density detection, haematoxylin and eosin (H&E) staining and immunohistochemical staining. We found that vascularization was enhanced by transfected myoblasts compared with non-transfected myoblasts. In addition, VEGF-165 and SDF-1 had a synergistic effect on vascularization during repair of skeletal muscle defects in vivo. In conclusion, we have combined myoblast-seeded collagen sponge with gene therapy, resulting in a promising approach for the construction of well-vascularized skeletal muscle.


Assuntos
Quimiocina CXCL12/biossíntese , Expressão Gênica , Terapia Genética , Músculo Esquelético , Mioblastos Esqueléticos , Neovascularização Fisiológica , Fator A de Crescimento do Endotélio Vascular/biossíntese , Animais , Quimiocina CXCL12/genética , Humanos , Masculino , Músculo Esquelético/irrigação sanguínea , Músculo Esquelético/lesões , Músculo Esquelético/metabolismo , Mioblastos Esqueléticos/metabolismo , Mioblastos Esqueléticos/transplante , Ratos , Ratos Sprague-Dawley , Transfecção , Fator A de Crescimento do Endotélio Vascular/genética
8.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 32(5): 504-8, 2014 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-25490832

RESUMO

OBJECTIVE: To investigate the expressions of serine-threonine kinase (Akt)/mammalian target of rapamycin (mTOR)/p70 S6K in oral squamous cell carcinoma (OSCC) and provide references for early diagnosis and prognosis evalua- tion of OSCC. METHODS: A total of 51 cases of OSCC, 10 cases of paracancerous mucosa, and 10 cases of normal oral mucosa were collected. The expressions of Akt/mTOR/p70 S6K in these cases were detected using the SP method of immunohisto- chemistry. The correlation between their expressions in OSCC was also analyzed. RESULTS: The positive expressions ofp-Akt, p-mTOR, and p70 S6K in OSCC were significantly higher than those in normal oral mucosa and paracancerous mucosa. The expressions of p-Akt, p-mTOR, and p70 S6K in OSCC were not correlated with age, gender, and clinical stage; by comparison, these expressions were correlated with lymph node metastasis and pathological grade. Strong positive correlations were also observed between the expressions ofp-Akt, p-mTOR, and p70 S6K in OSCC. CONCLUSION: Akt/mTOR/p70 S6K signaling molecules exhibit active expressions in OSCC and may be implicated in the occurrence and development of OSCC.


Assuntos
Neoplasias Bucais , Neoplasias de Células Escamosas , Proteínas Serina-Treonina Quinases , Sirolimo , Idoso , Animais , Humanos , Fosforilação , Proteínas Quinases , Proteínas Proto-Oncogênicas c-akt , Proteínas Quinases S6 Ribossômicas 70-kDa , Transdução de Sinais , Serina-Treonina Quinases TOR
9.
Int J Biol Markers ; 29(4): e440-4, 2014 Dec 09.
Artigo em Inglês | MEDLINE | ID: mdl-25385242

RESUMO

We describe a case of postradiation chondrosarcoma after basal cell carcinoma treatment. At the time he presented, the patient was a 35-year-old man who had received radiotherapy at a dose of 70 Gy for 8 weeks. Six months after radiation treatment, a rapidly growing mass at the upper right alveolar ridge of the gums, where radiation had been given, was diagnosed as chondrosarcoma. Generally, chondrosarcoma occurs after a latency period of several years following radiation. However, there are a few relevant reports indicating that maxillofacial chondrosarcoma can develop after radiotherapy for basal cell carcinoma, with a short latency of 6 months. We hypothesize that the dosage and treatment time of radiation may have played a role in the opening/closing of the Hh-signaling pathway in the case of this patient.


Assuntos
Neoplasias Ósseas/patologia , Condrossarcoma/patologia , Ossos Faciais/patologia , Neoplasias Induzidas por Radiação/patologia , Adulto , Neoplasias Ósseas/cirurgia , Carcinoma Basocelular/patologia , Carcinoma Basocelular/radioterapia , Condrossarcoma/cirurgia , Ossos Faciais/efeitos da radiação , Humanos , Masculino , Neoplasias Induzidas por Radiação/cirurgia
10.
World J Stem Cells ; 6(4): 491-6, 2014 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-25258671

RESUMO

Mesenchymal stem cells (MSCs), multipotential cells that reside within the bone marrow, can be induced to differentiate into various cells, such as osteoblasts, adipocytes, chondrocytes, vascular endothelial progenitor cells, and other cell types. MSCs are being widely studied as potential cell therapy agents due to their angiogenic properties, which have been well established by in vitro and in vivo researches. Within this context, MSCs therapy appears to hold substantial promise, particularly in the treatment of conditions involving skin grafts, pedicle flaps, as well as free flaps described in literatures. The purpose of this review is to report the new advances and mechanisms underlying MSCs therapy against skin flaps necrosis.

11.
J Clin Pathol ; 66(9): 758-63, 2013 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-23723304

RESUMO

OBJECTIVE: To investigate the expression of inhibitor of differentiation/DNA binding (Id-1), phosphatidylinositol-3-kinase/protein kinase B pathway proteins and hyperthermia-associated protein and their association with various clinicopathological factors in oral squamous cell cancer (OSCC), and explore the relationship among them in OSCC. METHODS: Id-1, phosphorylated protein kinase B (p-Akt), phosphorylated glycogen synthase kinase 3ß (p-GSK3ß) and phosphorylated heat shock factor 1 (p-HSF1) expression were assessed immunohistochemically in 76 OSCC. RESULTS: Id-1 (73.8%), p-Akt (65.8%), p-GSK3ß (60.5%) and p-HSF1 (75%) were found to be overexpressed in most of the oral cancer samples tested, and the expressions of them are correlated with advanced clinical stage, metastasis and recurrence (p<0.01), but there is no apparent relationship with gender, age, differentiation and habits (p>0.05). Survival curves show that the survival of patients with high Id-1, p-Akt, p-GSK3ß and p-HSF1 expression was significantly worse than those with low Id-1, p-Akt, p-GSK3ß and p-HSF1 expression (p=0.000). Id-1 expression was significantly higher in cases with high expression of p-Akt, p-GSK3ß and p-HSF1 than in those with low expression (p=0.002, p=0.003, p=0.001). CONCLUSIONS: This study revealed that there was a positive correlation between Id-1 expression and the expression of p-Akt, p-GSK3ß and p-HSF1. The inhibition of Id-1 expression can improve the efficacy of hyperthermia in OSCC.


Assuntos
Carcinoma de Células Escamosas/metabolismo , Proteínas de Ligação a DNA/metabolismo , Proteína 1 Inibidora de Diferenciação/metabolismo , Neoplasias Bucais/metabolismo , Fatores de Transcrição/metabolismo , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Carcinoma de Células Escamosas/patologia , Diferenciação Celular , Feminino , Quinase 3 da Glicogênio Sintase/metabolismo , Fatores de Transcrição de Choque Térmico , Humanos , Masculino , Pessoa de Meia-Idade , Neoplasias Bucais/patologia , Fosfatidilinositol 3-Quinases/metabolismo , Fosforilação , Proteínas Proto-Oncogênicas c-akt/metabolismo , Transdução de Sinais
12.
Med Oncol ; 30(3): 619, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23729269

RESUMO

Integrin-linked kinase (ILK) plays a key role in cell-excellular matrix interactions mediated by integrins and several growth factors, regulating cell proliferation, apoptosis, differentiation, and migration. It has also been implicated in the development and progression in several malignancies involving epithelial to mesenchymal transition (EMT). However, the correlations between ILK and EMT markers and the progression of salivary adenoid cystic carcinoma (SACC) have not been well elucidated. Here, by immunohistochemistry, we studied the expression of ILK, Snail, E-cadherin, and N-cadherin in 94 SACC specimens and analyzed their correlations with clinicopathologic characteristics. Positive expression of ILK protein was detected in 76.6 % of the tumors. Increased expression of ILK and Snail and decreased E-cadherin expression correlated strongly with tumor solid type (P = 0.017, P = 0.008, and P = 0.038, respectively), advanced TNM stage (P = 0.021, P = 0.034, and P = 0.009, respectively), and increased risk of recurrence (P = 0.023, P = 0.011, and P = 0.039, respectively) and distant metastasis (P < 0.001, P < 0.001, and P = 0.001, respectively). Moreover, up-regulation of Snail and N-cadherin and down-regulation of E-cadherin correlated significantly with ILK over-expression (P < 0.001, P = 0.001, and P < 0.001, respectively) and a neural-invasive phenotype (P = 0.017, P = 0.002, and P < 0.001, respectively). Taken together, our results suggest that ILK may have an important role in progression and metastasis of SACC, possibly through EMT involving up-regulation of Snail and consequent aberrant expression of E-cadherin and N-cadherin. ILK should be considered as a potential therapeutic molecular target for patients with SACC.


Assuntos
Biomarcadores Tumorais/genética , Carcinoma Adenoide Cístico/patologia , Transição Epitelial-Mesenquimal/fisiologia , Neoplasias Bucais/patologia , Proteínas Serina-Treonina Quinases/genética , Glândulas Salivares/patologia , Antígenos CD/genética , Caderinas/genética , Carcinoma Adenoide Cístico/genética , Linhagem Celular Tumoral , Progressão da Doença , Regulação para Baixo/genética , Transição Epitelial-Mesenquimal/genética , Feminino , Regulação Neoplásica da Expressão Gênica/genética , Humanos , Imuno-Histoquímica , Masculino , Pessoa de Meia-Idade , Neoplasias Bucais/genética , Metástase Neoplásica/genética , Metástase Neoplásica/patologia , Recidiva Local de Neoplasia/genética , Recidiva Local de Neoplasia/patologia , Fatores de Transcrição da Família Snail , Fatores de Transcrição/genética , Regulação para Cima/genética
13.
Clin Exp Metastasis ; 29(8): 957-69, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22638656

RESUMO

Integrin-linked kinase (ILK), an intracellular protein with serine/threonine protein kinase activities, plays a key role in integrin mediated cell-excellular matrix interactions, regulating cell proliferation, apoptosis, differentiation, and migration. ILK has been implicated in the development and progression in several malignancies. However, the role of ILK and ILK-mediated epithelial-mensenchymal transition (EMT) in the progression of oral squamous cell carcinoma (OSCC) has not been well understood. Here, by immunohistochemistry, we studied the expression of ILK, Snail, E-cadherin and N-cadherin in 98 primary OSCC specimens and analyzed their correlations with clinicopathologic profiles and clinical outcome. We also investigated the expression of ILK in 42 corresponding lymph node metastases. Positive expression of ILK protein was detected in 87.8 % of the primary tumors and 100 % of metastatic lesions. Increased ILK expression was correlated strongly with enhanced tumor invasion, higher tumor grade, advanced clinical stage, positive lymph node status and increased risk of recurrence. Higher ILK expression was also observed in lymph node metastases in comparison with the corresponding primary tumor. Moreover, up-regulation of Snail and N-cadherin and down-regulation of E-cadherin correlated significantly with both ILK over-expression and tumor invasion. Patients with higher ILK expression exhibited shorter disease-free survival while those with absent E-cadherin expression exhibited shorter overall and disease-free survival. Taken together, our results suggest that ILK may have an important role in progression and metastasis of OSCC, possibly through EMT involving up-regulation of Snail and consequent aberrant expression of E-cadherin and N-cadherin. ILK should be considered as a critical prognostic indicator for patients with OSCC.


Assuntos
Caderinas/metabolismo , Carcinoma de Células Escamosas/metabolismo , Neoplasias Bucais/metabolismo , Proteínas Serina-Treonina Quinases/metabolismo , Fatores de Transcrição/metabolismo , Carcinoma de Células Escamosas/secundário , Progressão da Doença , Intervalo Livre de Doença , Transição Epitelial-Mesenquimal , Feminino , Humanos , Metástase Linfática , Masculino , Pessoa de Meia-Idade , Metástase Neoplásica , Fatores de Transcrição da Família Snail
14.
Artigo em Chinês | MEDLINE | ID: mdl-22506478

RESUMO

OBJECTIVE: To construct tissue engineered skeletal muscle in vivo using glial cell derived neurotrophic factor (GDNF) genetically modified myoblast (Mb) on acellular collagen sponge with hypoglossal nerve implantation, and to observe whether structural or functional connection could be established between engineered tissue and motor nerve or not. METHODS: Mbs were isolated from 7 male Lewis rats at age of 2 days, cultured and genetically modified by recombinant adenovirus carrying GDNF cDNA (Mb(GDNF)). Calf skin-derived acellular collagen sponge was used as scaffold; cell adhesion was detected by scanning electron microscope after 24 hours. Hypoglossal nerve was implanted into Mb-scaffold complex (Mb group, n = 27) or Mb(GDNF)-scaffold complex (Mb(GDNF) group, n = 27) in 54 female Lewis rats at age of 8 weeks. HE staining was performed at 1, 6, and 12 weeks postoperatively, and immunohistochemistry staining and fluorescence in situ hybridization were used. RESULTS: Mb(GDNF) could highly expressed GDNF gene. Mb and Mb(GDNF) could adhere to the scaffold and grew well. HE staining showed tight junctions between implant and peripheral tissue with new muscle fiber and no distinguished line at 12 weeks in 2 groups. Immunohistochemistry staining showed that positive cells of myogenin and slow skeletal myosin were detected, as well as positive cells of acetylcholine receptor alpha1 at 1, 6, and 12 weeks. The positive cells of Y chromosome decreased with time. At 1, 6, and 12 weeks, the positive neurons were 261.0 +/- 6.6, 227.3 +/- 8.5, and 173.3 +/- 9.1, respectively in Mb(GDNF) group, and were 234.7 +/- 5.5, 196.0 +/- 13.5, and 166.7 +/- 11.7, respectively in Mb group; significant differences were found between 2 groups at 1 and 6 weeks (P < 0.05), no significant difference at 12 weeks (P > 0.05). CONCLUSION: Connection can be established between engineered tissue and implanted hypoglossal nerve. Recombinant GDNF produced by Mb(GDNF) might play a critical role in protecting central motor neurons from apoptosis by means of retrograde transportation.


Assuntos
Nervo Hipoglosso/transplante , Músculo Esquelético/cirurgia , Engenharia Tecidual , Animais , Células Cultivadas , Feminino , Fator Neurotrófico Derivado de Linhagem de Célula Glial , Sobrevivência de Enxerto , Masculino , Músculo Esquelético/citologia , Músculo Esquelético/inervação , Mioblastos/citologia , Neurônios , Ratos , Ratos Endogâmicos Lew
15.
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi ; 25(10): 1239-44, 2011 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-22069983

RESUMO

OBJECTIVE: To explore the human stromal cell-derived factor 1alpha (hSDF-1alpha) and human vascular endothelial growth factor 165 (hVEGF165) mRNA expressions of the transfected cells after hSDF-1alpha gene and hVEGF165 gene were transfected into rat myoblasts in vitro so as to lay a foundation for further study on the synergistic effects of 2 genes on tissue engineered skeletal muscle vascularization. METHODS: The myoblasts of 1-day-old Sprague Dawley rats were cultured and purified by trypsin digestion assay in vitro and were identified by immunohistochemistry staining of Desmin. Approximately 70%-80% of confluent myoblasts were transfected with enhanced green fluorescent protein (EGFP)-hSDF-1alpha and EGFP-hVEGF165 genes in vitro (transfected group) and were not transfected (control group). The expressions of hSDF-1alpha and hVEGF165 mRNA and protein in the transfected cells were detected by RT-PCR, ELISA, and Western blot respectively. RESULTS: The cultured cells were identified as myoblasts by immunohistochemistry staining of Desmin. The expression of green fluorescent protein was observed in transfected cells, indicating that hSDF-1alpha and hVEGF165 genes were transfected into myoblasts successfully. The mRNA and protein expressions of the 2 genes were positive in the transfected group by RT-PCR and Western bolt assay at 2, 4, 6, and 8 days after transfection, and were negative in the control group. The expressions of hSDF-1alpha and hVEGF165 showed a stable low level in the control group, but the expressions of the proteins increased at 2 days and then showed gradual downtrend with time in the transfected group by ELISA assay. There were significant differences in the expressions of hSDF-1alpha and hVEGF165 proteins between different time points in the transfected group, and between 2 groups (P < 0.05). CONCLUSION: hSDF-1alpha and hVEGF165 genes are successfully transfected into myoblasts in vitro, and mRNA and proteins of hSDF-1alpha and hVEGF165 can be expressed in the transfected myoblasts, which may provide the experimental evidence for the expressions of hSDF-1alpha and hVEGF165 mRNA and proteins in vivo successfully.


Assuntos
Quimiocina CXCL12/genética , Mioblastos/metabolismo , Transfecção , Fator A de Crescimento do Endotélio Vascular/genética , Animais , Células Cultivadas , Humanos , Masculino , RNA Mensageiro/genética , Ratos , Ratos Sprague-Dawley
16.
Int J Oral Sci ; 3(3): 141-52, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21789963

RESUMO

This article provides an overview of metastases to jaws (MJ), mainly concerning the differences between American and Chinese patients, and exploring the relationship between the primary tumors' prevalence (PTP) and constituent ratio of MJ. Information concerning of 399 MJ cases in 215 papers, including one new case in our hospital, was subjected to statistic analysis. The main clinical features of MJ, such as constituent ratio of PTP and that of MJ, metastatic sites, treatments, and prognosis were summarized. Breast, lung, kidney, prostate and thyroid (in descending order) were the leading primary sites of MJ. Furthermore, the constituent ratio of MJ was found to be correlated with that of PTP in all subjects including American and Chinese subjects in our study. As to metastatic sites in the mandible, a specific "M" shaped pattern appeared regardless of the tumor type or constituent ratios of MJ were in all subjects. Almost all subjects received traditionally palliative treatments, and the prognosis was quite poor. The PTP had a significant impact on the constituent ratio of MJ. However, it was the properties of the microenvironment rather than characteristics or constituent ratios of tumor cells, that decided the metastatic sites in various tumor subjects.


Assuntos
Neoplasias Maxilomandibulares/secundário , Metástase Neoplásica/fisiopatologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Neoplasias da Mama/patologia , Distribuição de Qui-Quadrado , Criança , Pré-Escolar , Feminino , Humanos , Lactente , Neoplasias Maxilomandibulares/mortalidade , Neoplasias Maxilomandibulares/terapia , Neoplasias Renais/patologia , Neoplasias Hepáticas/patologia , Neoplasias Pulmonares/patologia , Masculino , Pessoa de Meia-Idade , Cuidados Paliativos , Prognóstico , Neoplasias da Próstata/patologia , Estatísticas não Paramétricas , Análise de Sobrevida , Neoplasias da Glândula Tireoide/patologia , Adulto Jovem
17.
Hum Pathol ; 42(10): 1514-23, 2011 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-21531000

RESUMO

Hepatocyte growth factor has been identified as a lymphangiogenic factor in experimental animal models. However, the correlation between hepatocyte growth factor or c-Met expression and lymphangiogenesis in human spontaneous tumors has been rarely reported, and the distribution pattern of c-Met on tumor-related lymphatic vessels remains to be further investigated. Lymphatic vessel density, lymphatic invasion, the expression of hepatocyte growth factor, c-Met, and vascular endothelial growth factor C proteins were evaluated by immunohistochemistry in 76 cases of oral squamous cell carcinoma. The distribution of c-Met on lymphatic endothelium was examined. High expression of c-Met in tumor cells was significantly associated with advanced clinical stage (P = .045), high expression of vascular endothelial growth factor-C (P < .001), higher peritumoral lymphatic vessel density (P = .003), higher incidence of peritumoral lymphatic invasion (P = .032), and positive lymph node status (P = .005), in spite of its negative expression on most lymphatic vessels. Patients with high-c-Met expression tumors exhibited shorter overall survival and disease-free survival (P < .001 and P = .010, respectively). Taken together, our results provide indirect evidence for an association and possible regulatory link of c-Met with the lymphangiogenic factor, vascular endothelial growth factor C, and, by extension, with lymphangiogenesis and lymph node metastasis, suggesting important prognostic significance of c-Met for patients with oral squamous cell carcinoma.


Assuntos
Biomarcadores Tumorais/metabolismo , Carcinoma de Células Escamosas/metabolismo , Linfangiogênese , Neoplasias Bucais/metabolismo , Proteínas Proto-Oncogênicas c-met/metabolismo , Fator C de Crescimento do Endotélio Vascular/metabolismo , Carcinoma de Células Escamosas/diagnóstico , Carcinoma de Células Escamosas/patologia , Células Endoteliais/metabolismo , Endotélio Linfático/metabolismo , Feminino , Fator de Crescimento de Hepatócito/metabolismo , Humanos , Metástase Linfática , Vasos Linfáticos/metabolismo , Vasos Linfáticos/patologia , Masculino , Neoplasias Bucais/diagnóstico , Neoplasias Bucais/patologia , Recidiva Local de Neoplasia , Prognóstico , Análise de Sobrevida
18.
Ann Plast Surg ; 66(1): 92-7, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21042172

RESUMO

Mesenchymal stem cells (MSCs) have the potential for differentiating into vascular endothelial cells. Stromal cell-derived factor-1α (SDF-1α) plays an important role in neovascularization of ischemic flaps. The authors evaluated the feasibility of applying MSCs transduced by SDF-1α gene to the treatment of early and partial ischemic free flaps survival. MSCs were isolated from Lewis rats and cultured in vitro. Recombinant adenovirus encoding SDF-1α gene (Ad-SDF-1α) was transduced into the MSCs. Lewis rats that underwent epigastric free flaps based on medial and lateral branches of superficial inferior epigastric vessels and femoral vessels were equally randomized into 4 groups, and injected with Ad-SDF-1α-transduced MSCs, MSCs, Ad-SDF-1α, and normal saline, respectively. Gene transduction, flaps survival, neovascularization, and expression level of SDF-1a protein were detected. The results showed that Ad-SDF-1α-transduced MSCs expressed higher SDF-1α both in vitro and in vivo, yielded more survival area, and resulted in higher neovascularization than any other groups. Interestingly, the necrotic sites of all free flaps were in the proximal end rather than in the distal end. In conclusion, Ad-SDF-1α-transduced MSCs can increase neovascularization of early and partial ischemic free flaps and augment the surviving areas.


Assuntos
Quimiocina CXCL12/genética , Sobrevivência de Enxerto/genética , Transplante de Células-Tronco Mesenquimais/métodos , Células-Tronco Mesenquimais/metabolismo , Neovascularização Fisiológica/genética , Retalhos Cirúrgicos/irrigação sanguínea , Transdução Genética/métodos , Animais , Isquemia/genética , Isquemia/cirurgia , Ratos , Ratos Endogâmicos Lew
19.
J Zhejiang Univ Sci B ; 11(10): 745-53, 2010 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-20872981

RESUMO

Mucoepidermoid carcinoma undergoes uniquely vigorous angiogenic and neovascularization processes, possibly due to proliferation of vascular endothelial cells (ECs) induced by mucoepidermoid carcinoma cells (MCCs) in their three-dimensional (3D) microenvironment. To date, no studies have dealt with tumor cells and vascular ECs from the same origin of mucoepidermoid carcinoma using the in vitro 3D microenvironment model. In this context, the current research aims to observe neovascularization with mucoepidermoid carcinoma microvascular ECs (MCMECs) conditioned by the microenvironment in the 3D collagen matrix model. We observed the growth of MCMECs purified by immunomagnetic beads and induced by MCCs, and characteristics of tubule-like structures (TLSs) formed by induced MCMECs or non-induced MCMECs. The assessment parameters involved the growth curve, the length, the outer and inner diameters, and the wall thickness of the TLSs, and the cell cycle. Results showed that MCCs induced formation of the TLSs in the 3D collagen matrix model. A statistically significant difference was noted regarding the count of TLSs between the control group and the induction group on the 4th day of culture (t=5.00, P=0.001). The outer and inner diameters (t(1)=5.549, P(1)=0.000; t(2)=10.663, P(2)=0.000) and lengths (t=18.035, P=0.000) of the TLSs in the induction group were statistically significant larger than those in the control group. The TLSs were formed at the earlier time in the induction group compared with the control group. It is concluded that MCCs promote growth and migration of MCMECs, and formation of the TLSs. The 3D collagen matrix model with MCMECs induced by MCCs in the current research may be a favorable choice for research on pro-angiogenic factors in progression of mucoepidermoid carcinoma.


Assuntos
Carcinoma Mucoepidermoide/patologia , Colágeno/fisiologia , Células Endoteliais/citologia , Neovascularização Patológica/patologia , Animais , Carcinoma Mucoepidermoide/irrigação sanguínea , Ciclo Celular , Linhagem Celular Tumoral , Humanos , Separação Imunomagnética , Camundongos
20.
J Craniofac Surg ; 21(4): 1247-9, 2010 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-20613598

RESUMO

Such a giant anterior skull base mucocele involving the orbito-ethmoido-maxillo-fronto sinuses is a rare case. This patient complained of a right orbital mass for 18 years, and he was recommended to our hospital for treatment. His history, physical examination, laboratory study results, diagnosis, and treatment are reported in detail. In conclusion, we show that modified transcranial pathway and ingenious assistant incision can get the utmost exposure and can obtain a satisfactory aesthetic result and that anterior skull base reconstruction with the galeal-frontalis-pericranial flap based on the temporalis myofascial flap for the first time can successfully prevent life-threatening complications from happening.


Assuntos
Fossa Craniana Anterior/cirurgia , Mucocele/cirurgia , Procedimentos Cirúrgicos Reconstrutivos/métodos , Neoplasias da Base do Crânio/cirurgia , Retalhos Cirúrgicos , Fasciotomia , Humanos , Masculino , Pessoa de Meia-Idade , Músculo Temporal/transplante
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