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1.
Microbiol Spectr ; : e0493222, 2023 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-36622166

RESUMO

The immune regulator galectin-9 (Gal-9) is commonly involved in the regulation of cell proliferation, but with various impacts depending on the cell type. Here, we revealed that Gal-9 expression was persistently increased in Epstein-Barr virus (EBV)-infected primary B cells from the stage of early infection to the stage of mature lymphoblastoid cell lines (LCLs). This sustained upregulation paralleled that of gene sets related to cell proliferation, such as oxidative phosphorylation, cell cycle activation, and DNA replication. Knocking down or blocking Gal-9 expression obstructed the establishment of latent infection and outgrowth of EBV-infected B cells, while exogenous Gal-9 protein promoted EBV acute and latent infection and outgrowth of EBV-infected B cells at the early infection stage. Mechanically, stimulator of interferon gene (STING) activation or signal transducer and activator of transcription 3 (STAT3) inhibition impeded the outgrowth of EBV-infected B cells and promotion of Gal-9-induced lymphoblastoid cell line (LCL) transformation. Accordingly, Gal-9 expression was upregulated by forced EBV nuclear antigen 1 (EBNA1) expression in 293T cells in vitro. Clinical data showed that Gal-9 expression in B-cell lymphomas (BCLs) correlated positively with EBNA1 and disease stage. Targeting Gal-9 slowed LCL tumor growth and metastasis in xenografted immunodeficient mice. These findings highlight an oncogenic role of Gal-9 in EBV-associated BCLs, indicating that Gal-9 boosts the transformation of EBV-infected B cells. IMPORTANCE The cross talk between Epstein-Barr virus (EBV) and the host cell transcriptome assumes important roles in the oncogenesis of EBV-associated malignancies. Here, we first observed that endogenous Gal-9 expression was persistently increased along with an overturned V-type change in antivirus signaling during the immortalization of EBV-transformed B cells. Upregulation of Gal-9 promoted the outgrowth and latent infection of EBV-infected B cells, which was linked to B-cell-origin tumors by suppressing STING signaling and subsequently promoting STAT3 phosphorylation. EBV nuclear antigen EBNA1 induced Gal-9 expression and formed a positive feedback loop with Gal-9 in EBV-infected B cells. Tumor Gal-9 levels were positively correlated with disease stage and EBNA1 expression in patients with B-cell lymphomas (BCLs). Targeting Gal-9 slowed the growth and metastases of LCL tumors in immunodeficient mice. Altogether, our findings indicate that Gal-9 is involved in the lymphomagenesis of EBV-positive BCLs through cross talk with EBNA1 and STING signals.

2.
Mar Pollut Bull ; 187: 114567, 2023 Jan 12.
Artigo em Inglês | MEDLINE | ID: mdl-36640495

RESUMO

Resting cysts of dinoflagellates seed harmful algal blooms (HABs) and their geographic expansion, which makes it fundamentally important to obtain comprehensive inventories of dinoflagellate resting cysts in HABs-prone regions. The Yellow Sea (YS) of China has observed numerous outbreaks of dinoflagellate HABs with some novel species recorded recently indicating an underestimated HABs-causing species diversity. We report our investigation of dinoflagellate cysts of YS via an approach combining metabarcoding sequencing and single-cyst morpho-molecular identification, which identified many novel cyst species and a significant controlling effect of the Yellow Sea Cold Water Mass on cyst composition. The metabarcoding and single cyst-based sequencing detected 11 cyst species never being unambiguously reported in China, 10 never reported as cyst producers, and 3 HABs-causing species never reported from YS. Our detections of many potentially toxic or HABs-causative, particularly novel, cysts and distribution pattern provide important insights into the risks and ecology of dinoflagellate HABs.

3.
Artigo em Inglês | MEDLINE | ID: mdl-36673990

RESUMO

China, the largest country in vegetable supply, faces a transition to sustainable vegetable production to counteract resource waste and environmental pollution. However, there are knowledge gaps on the main constraints and how to achieve sustainable vegetable supply. Herein, we integrated the vegetable production and supply data in China, compared its current status with five horticulture-developed countries US, the Netherlands, Greece, Japan and South Korea, using data from the Food and Agriculture Organization (FAO) and National Bureau of Statistics of China, and predicted the vegetable supply in 2030 and 2050 by a model prediction. The vegetable supply in China increased from 592 g capita-1 d-1 in 1995 to 1262 g capita-1 d-1 in 2018 and will keep constant in 2030 and 2050. Compared to the five countries, the greater vegetable supply is primarily achieved by higher harvested areas rather than higher yield. However, it is predicted that the harvested areas will decrease by 13.6% and 24.7% in 2030 and 2050. Instead, steady increases in vegetable yield by 11.8% and 28.3% are predicted for this period. The high vegetable supply and greater vegetable preference indicated by the high vegetable-to-meat production ratio cannot guarantee recommended vegetable intake, potentially due to the high rate of vegetable loss and waste. Under the scenarios of decreased vegetable loss and waste, the harvested area will decrease by 37.3-67.2% in 2030 and 2050. This study points out that the sustainable transition of Chinese vegetable supply can be realized by enhancing yield and limiting vegetable loss and waste instead of expanding the harvested area.


Assuntos
Agricultura , Verduras , China , Poluição Ambiental , Horticultura , Abastecimento de Alimentos
4.
Food Chem ; 412: 135533, 2023 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-36716630

RESUMO

The effect and mechanism of sodium hexametaphosphate (SHMP) on polyphenol oxidase (PPO) enzymatic browning in yellow alkaline noodles (YAN) were investigated. The browning degree and PPO activity in YAN or PPO solutions decreased with the SHMP concentrations increased. Variations in pH values (pH 7-8.5) adjusted by HCl or acetic acid slightly affected the PPO activity, but SHMP inhibited PPO activity more pronounced. The inhibition of SHMP on PPO activity was irreversible. SHMP formed coordinate covalent bonds with Cu2+ to make PPO inactive. HPLC analysis revealed that SHMP reduced the browning products and led to the color of PPO-catechol systems being lightened. Furthermore, SHMP inhibited browning by hampering the auto-oxidization of intermediate products due to the change in pH value. Besides, the HPLC chromatogram, UV-vis spectrum, and mass spectrometry revealed that SHMP could convert melanin (m/z 248.97, 723.5, and 836.58) to light-colored substances (m/z 137.11).

5.
Opt Lett ; 48(2): 391-394, 2023 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-36638465

RESUMO

Optical waveguide amplifiers are essential devices in integrated optical systems. Their gain bandwidths directly determine the operating wavelength of optical circuits. Due to the difficulty of developing wideband gain media, it has been a challenge to fabricate devices with broadband amplification capability, resulting in few reports on multi-band polymer waveguide amplifiers. Here, a polymer waveguide amplifier is demonstrated, which achieves loss compensation covering the whole (S + C) band by using NaYF4:Tm,Yb@NaYF4@NaYF4:Er nanoparticles (NPs)-doped SU-8 as the gain medium. The NPs with a layer-doped core-multishell structure not only provided two emitters required for (S + C)-band amplification, but also reduced the energy transfer (ET) between them. Under 980-nm excitation, the full width at half maximum (FWHM) of the emission peak of NPs reached 119 nm, and the relative gain in the (S + C) band was about 6-8 dB, successfully expanding the operating wavelength from single-band to multi-band.

6.
Artigo em Inglês | MEDLINE | ID: mdl-36689684

RESUMO

Reducing the interfacial defects of perovskite films is key to improving the performance of perovskite solar cells (PSCs). In this study, two kinds of perylene monoimide (PMI) derivative phosphonium bromide salts were designed and used as a multifunctional interface-modified layer in PSCs. These two molecules are inserted between SnO2 and perovskite to produce a bidirectional passivation effect. The interaction with SnO2 reduces the oxygen vacancy on the surface of SnO2 and tunes the energy level of the electron transport layer, making more matches with the perovskite layer. The modified layer can promote the growth of perovskite crystals and reduce the interfacial defects of the perovskite film. Furthermore, the power conversion efficiency (PCE) of PSCs increased from 19.49 to 22.85%, and the open-circuit voltage (VOC) increased from 1.06 to 1.14 V. At the same time, the PCE of the SnO2/PMI-TPP-based device remained 88% of the initial PCE after 240 h of continuous illumination. In addition, these two PMI derivatives with a quasi-planar structure can improve the flexibility of flexible PSCs. This study provided a new strategy for the interfacial modification of PSCs and a new insight into the application of flexible PSCs.

7.
Hepatology ; 2023 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-36683360

RESUMO

BACKGROUND AND AIMS: Deregulation of adenosine-to-inosine (A-to-I) editing by adenosine deaminase acting on RNA 1 (ADAR1) leads to tumor-specific transcriptome diversity with prognostic values for hepatocellular carcinoma (HCC). However, ADAR1 editase-dependent mechanisms governing liver cancer stem cell (LCSC) generation and maintenance have remained elusive. APPROACH AND RESULTS: RNA-seq profiling identified ADAR1-responsive recoding editing events in HCC and showed editing frequency of GLI1 rather than transcript abundance, was clinically relevant. Functional differences in LCSC self-renewal and tumor aggressiveness between wild-type (GLI1 wt ) and edited GLI1 (GLI1 R701G ) were elucidated. We showed that overediting of GLI1 induced an arginine-to-glycine (R701G) substitution, augmenting tumor-initiating potential and exhibiting a more aggressive phenotype. GLI1 R701G harbored weak affinity to SUFU; which in turn, promoted its cytoplasmic-to-nuclear translocation to support LCSC self-renewal by increased pluripotency gene expression. Moreover, editing predisposes to stabilized GLI1 by abrogating ß-TrCP-GLI1 interaction. Integrative analysis of single-cell transcriptome further revealed hyperactivated mitophagy in ADAR1-enriched LCSCs. GLI1 editing promoted a metabolic switch to oxidative phosphorylation (OXPHOS) to control stress and stem-like state via PINK1-Parkin-mediated mitophagy in HCC, thereby conferring exclusive metastatic and sorafenib-resistant capacities. CONCLUSIONS: Our findings demonstrate a novel role of ADAR1 as an active regulator for LCSCs properties via editing GLI1 in the highly heterogeneous HCC.

8.
Nat Commun ; 14(1): 489, 2023 Jan 30.
Artigo em Inglês | MEDLINE | ID: mdl-36717587

RESUMO

Vascular repair is considered a key restorative measure to improve long-term outcomes after ischemic stroke. N6-methyladenosine (m6A), the most prevalent internal modification in eukaryotic mRNAs, functionally mediates vascular repair. However, whether circular RNA SCMH1 (circSCMH1) promotes vascular repair by m6A methylation after stroke remains to be elucidated. Here, we identify the role of circSCMH1 in promoting vascular repair in peri-infarct cortex of male mice and male monkeys after photothrombotic (PT) stroke, and attenuating the ischemia-induced m6A methylation in peri-infarct cortex of male mice after PT stroke. Mechanically, circSCMH1 increased the translocation of ubiquitination-modified fat mass and obesity-associated protein (FTO) into nucleus of endothelial cells (ECs), leading to m6A demethylation of phospholipid phosphatase 3 (Plpp3) mRNA and subsequently the increase of Plpp3 expression in ECs. Our data demonstrate that circSCMH1 enhances vascular repair via FTO-regulated m6A methylation after stroke, providing insights into the mechanism of circSCMH1 in promoting stroke recovery.


Assuntos
RNA Circular , Acidente Vascular Cerebral , Masculino , Animais , Camundongos , Células Endoteliais/metabolismo , Adenosina/metabolismo , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Acidente Vascular Cerebral/genética , Acidente Vascular Cerebral/terapia , Infarto , Dioxigenase FTO Dependente de alfa-Cetoglutarato/genética , Dioxigenase FTO Dependente de alfa-Cetoglutarato/metabolismo
9.
Nanomaterials (Basel) ; 13(2)2023 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-36678055

RESUMO

Pressure sensors urgently need high-performance sensing materials in order to be developed further. Sensitivity and creep are regarded as two key indices for assessing a sensor's performance. For the design and optimization of sensing materials, an accurate estimation of the impact of several parameters on sensitivity and creep is essential. In this study, sensitivity and creep were predicted using the response surface methodology (RSM) and support vector regression (SVR), respectively. The input parameters were the concentrations of nickel (Ni) particles, multiwalled carbon nanotubes (MWCNTs), and multilayer graphene (MLG), as well as the magnetic field intensity (B). According to statistical measures, the SVR model exhibited a greater level of predictability and accuracy. The non-dominated sorting genetic-II algorithm (NSGA-II) was used to generate the Pareto-optimal fronts, and decision-making was used to determine the final optimal solution. With these conditions, the optimized results revealed an improved performance compared to the earlier study, with an average sensitivity of 0.059 kPa-1 in the pressure range of 0-16 kPa and a creep of 0.0325, which showed better sensitivity in a wider range compared to previous work. The theoretical sensitivity and creep were relatively similar to the actual values, with relative deviations of 0.317% and 0.307% after simulation and experimental verification. Future research for transducer performance optimization can make use of the provided methodology because it is representative.

10.
Allergol Immunopathol (Madr) ; 51(1): 77-83, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-36617825

RESUMO

Nedd4 family interacting protein 1 (Ndfip1) was first mentioned in an article in 2000. Since its discovery, related studies have shown that this protein is associated with apoptosis, neuroprotection, substance transport, ubiquitination, and immune regulation. It is noteworthy that the lack of Ndfip1 can lead to death in fetal mice. Researchers generally believe that the function of Ndfip1 is closely related to individual immune capacity and have published a large number of articles. However, a comprehensive classification of the immune regulatory function of Ndfip1 is still lacking. In this review, we will overview and discuss this new perspective, focusing on the role of Ndfip1 in the proliferation, differentiation, and cell activity of CD4+ T cells, CD8+ T cells, mast cells, and eosinophils. This review provides an updated summary of Ndfip1, which will unveil novel therapeutic targets. Finally, the conclusion is that Ndfip1 mainly plays a negative regulatory role in immune cells by maintaining the stability of the immune response and limiting its overexpression.


Assuntos
Linfócitos T CD8-Positivos , Ubiquitina-Proteína Ligases , Camundongos , Animais , Ubiquitina-Proteína Ligases/genética , Ubiquitina-Proteína Ligases/metabolismo , Proteínas de Transporte/metabolismo , Proteínas de Membrana/genética , Ubiquitinação
11.
JMIR Serious Games ; 11: e40130, 2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36645700

RESUMO

BACKGROUND: Chinese gamers use computer and mobile phone games widely. Consequently, concerns regarding the development of internet gaming disorder (IGD) in adolescents have been raised. However, only a few studies have focused on the influence of gaming devices on IGD. OBJECTIVE: This study aims to compare sociodemographic information, gaming use characteristics, personality traits, and gaming motivations between computer game users (CGUs) and mobile phone game users (MGUs), as well as identifying IGD predictors. METHODS: This was a cross-sectional study. A total of 3593 internet game players took part in an online survey, which included sociodemographic information, gaming patterns, gaming motivations, the Chinese version of the Video Game Dependency Scale, and the Chinese Big Five Personality Inventory brief version. The population was divided into 2 groups for comparison by mobile phone or computer use, and the IGD population was also compared within the 2 groups. RESULTS: There were significant differences between the 2 gaming device groups in the time (t2994=7.75, P<.001) and money (t2994=5.11, P<.001) spent on gaming and in internet game addiction scores (t2994=3.68, P<.001). Individuals using different gaming devices had different game motivations and personality traits and preferred different genres of games. Results showed that IGD predictors were different for the 2 groups, for example, strategy (odds ratio [OR] 4.452, 95% CI 1.938-10.227; P<.001) and action shooter (OR 3.725, 95% CI 1.465-9.474; P=.01) games increased the risk for MGUs. CONCLUSIONS: Gaming devices should be considered during early identification, such as long daily gaming time, much money spent on gaming, neuroticism, and conscientiousness. In addition, more research should be conducted on new gaming devices and IGD treatment.

12.
Asia Pac J Oncol Nurs ; 10(2): 100171, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36632445

RESUMO

Objective: Little is known about the measurement invariance (MI) of resilience instruments in cancer care. This study was designed to examine MI of 10-Item Resilience Scale (RS-SC-10) in Americans and Chinese with cancer using propensity score-based multidimensional item response theory (MIRT) analysis. Methods: A sample of 924 patients were enrolled in the Be Resilient to Cancer trial involving 1 hospital in America and 3 hospitals in China. Data were collected from the RS-SC-10 and Hospital Anxiety and Depression Scale. Propensity score matching and MIRT were performed to evaluate Differential Item Function. Integrated Discrimination Improvement and Net Reclassification Improvement were used to indirectly estimate the MI through incremental prediction ability of MIRT-based score over total score. Results: RS-SC-10 retained 10 items with monotonous thresholds and its original two-factor structure. Nonuniform Differential Item Function was recognized in Item 4 (P â€‹= â€‹0.0011, Δ%ß1 â€‹= â€‹4.15%) and Item 8 (P â€‹= â€‹0.0017, Δ%ß1 â€‹= â€‹5.99%). Net Reclassification Improvement ranged from 9.04% to 35.01%, and Integrated Discrimination Improvement ranged from 8.82% to 20.60%. Conclusions: Although partial MI has been identified between Americans and Chinese, RS-SC-10 remains a critical indicator to emotional distress in cancer care.

13.
J Mater Chem B ; 2023 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-36651433

RESUMO

In this work, a ratiometric fluorescent nanoplatform for the detection of ascorbic acid (AA) was constructed based on the Ag2S quantum dots (QDs) and multifunctional hydroxyl cobalt oxide nanoflakes (CoOOH NFs). Ag2S QDs can be assembled on the surface of CoOOH NFs by electrostatic adsorption, resulting in the quenching of the NIR fluorescence emission of Ag2S QDs at 680 nm effectively through the inner filter effect (IFE). o-Phenylenediamine (OPD), a common substrate of oxidase-like (OXD) mimic, is rapidly oxidized into the fluorescent product of 2,3-diaminophenazine (DAP) with the appearance of an emission peak at 575 nm under the catalysis of CoOOH NFs. After AA was added, the fluorescence emission of DAP declined because of the decline in the OXD-like activity of CoOOH NFs due to the transformation of Co2+. Simultaneously, Ag2S QDs were released, accompanied by the recovery of red fluorescence. These two fluorescent signals can be excited at the same excitation wavelength, simplifying the detection procedure. Using F575/F680 as the readout, the quantification of AA can be realized with the linear range and detection limit of 0.2 µM-20 mM and 0.014 µM, respectively. The ratiometric fluorescence sensor can be effectively used to determine the content of AA in real samples such as juice and serum. This work integrates the in-situ formation of the fluorescent species via the catalysis of the nanozyme and the redox reaction to destroy the CoOOH NFs nanozyme as well as the two dimensional nanoflake induced turn-off-on strategy for Ag2S QDs, which provides a specific strategy for the selective detection of AA and may offer a reliable approach for the construction of other biosensing platforms.

14.
Acta Biomater ; 157: 408-416, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36549634

RESUMO

Photothermal therapy has become a promising approach as precision medicine to allow spatial control of therapeutic effect only in the site of interest. However, the full potential of PTT has not been realized due to the lack of simple photosensitizers (PSs) that can overcome multistage biological barriers and improve theranostic efficiency. Here, we develop a small molecule-based PS to enhance tumor-specific PTT by programming multistage transport and activation properties in molecular architecture. This PS can self-assemble into stable nanoparticles that accumulate passively in tumor, and then actively internalize through ligand-mediated endocytosis. Subsequently, the programmable degradable linkers are selectively cleaved, enabling size shrinkage for better tumor penetration, binding albumin to enhance the near-infrared fluorescence for low-background imaging, and activating photothermal conversion for tumor suppression. The self-delivery process can be programmed, representing the first multistage small-molecule nano-photosensitizer that overcomes multiple biological barriers and improves the PTT index of tumor. STATEMENT OF SIGNIFICANCE: Photothermal therapy has become a promising approach as precision medicine, but has not been realized due to the lack of simple photosensitizers that can overcome multistage biological barriers and improve theranostic efficiency. In this contribution, we solve this dilemma by developing a small molecule-based photosensitizer by programming multistage transport and activation properties in molecular architecture, which could self-assemble into stable nanoparticles that accumulate passively in tumor, and actively internalized through ligand-mediated endocytosis. Subsequently, the programmable activation by ROS triggered size reduction for tumor penetration and minimized the phototoxicity to normal tissue. The activatable fluorescence and photothermal properties made the photosensitizer intrinsically suitable for multimodal imaging-guided PTT, providing a promising supramolecular nanomedicine towards tumor precise diagnosis and therapy.


Assuntos
Nanopartículas , Neoplasias , Humanos , Fármacos Fotossensibilizantes/farmacologia , Fármacos Fotossensibilizantes/uso terapêutico , Fármacos Fotossensibilizantes/química , Terapia Fototérmica , Linhagem Celular Tumoral , Ligantes , Nanopartículas/uso terapêutico , Nanopartículas/química , Neoplasias/tratamento farmacológico , Imagem Multimodal , Nanomedicina Teranóstica/métodos , Fototerapia/métodos
15.
Inorg Chem ; 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36453837

RESUMO

Catalytic hydrogenation reduction based on sodium borohydride (NaBH4) has gained attention as an appealing "one-stone-two-birds" approach for the simultaneous elimination of nitroaromatic pollutants and the production of high-value aminoaromatics under mild conditions. However, the slow kinetics of NaBH4 dissociation on the surface of catalysts restrict the catalytic hydrogenation reduction efficiency. Herein, we report an intelligent localized sulfidation strategy for an in situ implantation of Bi2S3 nanorods within quasi-Bi-MOF architectures (Bi2S3@quasi-Bi-MOF) by fine-tuning the pyrolysis temperature. In this novel Bi2S3@quasi-Bi-MOF, the porous quasi-Bi-MOF enables efficient adsorption of BH4- and 4-nitrophenol (4-NP), while Bi2S3 facilitates the BH4- dissociation to form Hads* species adsorbed on the catalyst surface. Benefiting from the synergistic structure, Bi2S3@quasi-Bi-MOF exhibits excellent performance for the catalytic reduction of 4-NP, delivering a high turnover frequency (TOF) of 1.67 × 10-4 mmol mg-1 min-1 and an extremely high normalized rate constant (knor) of 435298 s-1 g-1. The kinetic analysis and electrochemical tests indicate that this catalytic hydrogenation reduction follows the Langmuir-Hinshelwood mechanism. This study enriches the synthetic strategy of MOF-based derivatives and offers a new catalytic platform for hydrogenation reduction reactions.

16.
Front Immunol ; 13: 1044299, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36505464

RESUMO

Hemolytic disease in grass carp (C. idella) leads to hemolysis in vivo, releasing damage-related molecular patterns (DAMPs) hemoglobin (Hb; which is rapidly oxidized to Hb-Fe3+ and Hb-Fe4+) and generating a high level of reactive oxygen species (ROS) that cause oxidative damage. However, the effect of cell-free Hb on tissue cells of grass carp has yet to be elucidated. In this study, western blotting (WB) and immunofluorescence analysis (IFA) results showed that PHZ-induced hemolysis caused Hb and iron accumulation, increased the production of ROS and resulted in apoptosis in head kidney and middle kidney of the grass carp. Quantitative real-time PCR (qRT-PCR), WB, and IFA revealed that PHZ-induced hemolysis significantly upregulated the expression of inflammation-related genes through activation of the NF-κB signaling pathway. To further explore the effect of Hb, three forms of Hb (Hb, MetHb, and FerrylHb) were prepared. The incubation with the different forms of Hb and heme markedly upregulated the expression of cytokine genes through NF-κB signaling pathway, which was further confirmed by a specific inhibitor (caffeic acid phenethyl ester, CAPE). Flow cytometry analysis data showed that the stimulation of different forms of Hb and heme increased the production of ROS, and resulted in apoptosis. In summary, our data suggest that the excess cell-free Hb released during hemolysis modulates the inflammatory response through activation of the NF-κB signaling pathway and causes cell oxidative damage and apoptosis.


Assuntos
Carpas , NF-kappa B , Animais , Transdução de Sinais , Inflamação , Estresse Oxidativo , Hemoglobinas , Hemólise , Heme
17.
Front Chem ; 10: 1103704, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36569959

RESUMO

Two-dimensional antiferromagnetic semiconductors have triggered significant attention due to their unique physical properties and broad application. Based on first-principles calculations, a novel two-dimensional (2D) antiferromagnetic material MnSi2N4 monolayer is predicted. The calculation results show that the two-dimensional MnSi2N4 prefers an antiferromagnetic state with a small band gap of 0.26 eV. MnSi2N4 has strong antiferromagnetic coupling which can be effectively tuned under strain. Interestingly, the MnSi2N4 monolayer exhibits a half-metallic ferromagnetic properties under an external magnetic field, in which the spin-up electronic state displays a metallic property, while the spin-down electronic state exhibits a semiconducting characteristic. Therefore, 100% spin polarization can be achieved. Two-dimensional MnSi2N4 monolayer has potential application in the field of high-density information storage and spintronic devices.

18.
Cells ; 11(23)2022 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-36497042

RESUMO

OBJECTIVE: To study the effect and mechanism of the Clostridium metabolite p-Cresol sulfate (PCS) in primary biliary cholangitis (PBC). METHODS: Gas chromatography-mass spectrometry (GC-MS) was used to detect differences in tyrosine, phenylalanine, tryptophan, PCS, and p-Cresyl glucuronide (PCG) between the serum of PBC patients and healthy controls. In vivo experiments, mice were divided into the normal control, PBC group, and PBC tyrosine group. GC-MS was used to detect PCS and PCG. Serum and liver inflammatory factors were compared between groups along with the polarization of liver Kupffer cells. Additionally, PCS was cultured with normal bile duct epithelial cells and Kupffer cells, respectively. PCS-stimulated Kupffer cells were co-cultured with lipopolysaccharide-injured bile duct epithelial cells to detect changes in inflammatory factors. RESULTS: Levels of tyrosine and phenylalanine were increased, but PCS level was reduced in PBC patients, with PCG showing a lower concentration distribution in both groups. PCS in PBC mice was also lower than those in normal control mice. After oral administration of tyrosine feed to PBC mice, PCS increased, liver inflammatory factors were decreased, and anti-inflammatory factors were increased. Furthermore, Kupffer cells in the liver polarized form M1 transitioned to M2. PCS can damage normal bile duct epithelial cells and suppress the immune response of Kupffer cells. But PCS protects bile duct epithelial cells damaged by LPS through Kupffer cells. CONCLUSIONS: PCS produced by Clostridium-metabolized tyrosine reduced PBC inflammation, suggesting that intervention by food, or supplementation with PCS might represent an effective clinical strategy for treating PBC.


Assuntos
Cirrose Hepática Biliar , Camundongos , Animais , Cirrose Hepática Biliar/tratamento farmacológico , Cirrose Hepática Biliar/metabolismo , Células de Kupffer/metabolismo , Sulfatos , Inflamação , Lipopolissacarídeos/farmacologia , Tirosina , Clostridium , Fenilalanina
19.
Int J Mol Sci ; 23(24)2022 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-36555471

RESUMO

The activation of monocytes and their trans-differentiation into macrophages are critical processes of the immune response. Prior work has characterized the differences in the expression between monocytes and macrophages, but the transitional process between these cells is poorly detailed. Here, we analyzed the temporal changes of the transcriptome during trans-differentiation of primary human monocytes into M0 macrophages. We find changes with many transcription factors throughout the process, the vast majority of which exhibit a maximally different expression at the intermediate stages. A few factors, including AP-1, were previously known to play a role in immunological transitions, but most were not. Thus, these findings indicate that this trans-differentiation requires the dynamic expression of many transcription factors not previously discussed in immunology, and provide a foundation for the delineation of the molecular mechanisms associated with healthy or pathological responses that involve this transition.


Assuntos
Monócitos , Fatores de Transcrição , Humanos , Monócitos/metabolismo , Fatores de Transcrição/metabolismo , Macrófagos/metabolismo , Diferenciação Celular/fisiologia , Transdiferenciação Celular/genética
20.
Artigo em Inglês | MEDLINE | ID: mdl-36498230

RESUMO

Under the context of digital economy, agricultural production will be promoted by implementing the strategy of digital rural construction and giving full play to the role of digital factor productivity. This study systematically explains the mechanism of how digital rural construction affects the efficiency of green transformation for cultivated land use. The panel data of 30 provinces in China from 2011 to 2020 are analyzed through two-way fixed effect, spatial Dubin model and other methods, so as to better understand the impact of digital rural construction on the efficiency of green transformation for cultivated land use and its spillover effect. It is discovered in the study that digital rural construction is effective in enhancing the efficiency of green transformation for regional cultivated land use, and that this promoting effect stands multiple robustness tests. According to the heterogeneity analysis, the promoting effect of digital rural construction is more significant in the eastern region and among the samples with high green transformation efficiency of cultivated land use. In addition to improving the efficiency of green transformation for cultivated land use in the region, digital rural construction can also produce a positive spatial spillover effect to a significant extent. On this basis, the targeted policy recommendations are made in this paper. The first one is to improve the efficiency of green transformation for cultivated land use by accelerating the process of digital rural construction. The second one is to pay close attention to the differences in the process of digital rural construction. The third one is to better understand the "welfare sharing" characteristics of digital rural construction. The last one is to establish a mechanism of regional cooperation.


Assuntos
Agricultura , Conservação dos Recursos Naturais , Humanos , China , Eficiência , População Rural , Desenvolvimento Econômico
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