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1.
Nanoscale ; 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-32227011

RESUMO

Lithium-ion batteries (LIBs) play an important role in modern society. The low capacity of graphite cannot meet the demands of LIBs calling for high power and energy densities. Silicon (Si) is one of the most promising materials instead of graphite, because of its high theoretical capacity, low discharge voltage, low cost, etc. However, Si shows low conductivity of both ions and electrons and exhibits a severe volume change during cycles. Fabricating nano-sized Si and Si-based composites is an effective method to enhance the electrochemical performance of LIB anodes. Using a small size of Si nanoparticles (SiNPs) is likely to avoid the cracking of this material. One critical issue is to disclose different types and electrochemical effects of various coupled materials in the Si-based composites for anode fabrication and optimization. Hence, this paper reviews diverse SiNP-based composites for advanced LIBs from the perspective of composition and electrochemical effects. Almost all kinds of materials that have been coupled with SiNPs for LIB applications are summarized, along with their electrochemical influences on the composites. The integrated materials, including carbon materials, metals, metal oxides, polymers, Si-based materials, transition metal nitrides, carbides, dichalcogenides, alloys, and metal-organic frameworks (MOFs), are comprehensively presented.

2.
World Neurosurg ; 2020 Mar 07.
Artigo em Inglês | MEDLINE | ID: mdl-32156592

RESUMO

BACKGROUND: The oblique lumbar interbody fusion (OLIF) procedure may reduce many of the risks and limitations associated with lateral lumbar interbody fusion (LLIF). However, little is known about the biomechanical performance of various fixation constructs after OLIF.This study aimed to explore the stability of various fixation options for OLIF by using finite element analysis based on three-dimensional scanning models. METHODS: Six validated finite element models of the L3-L5 segment were reconstructed via computed tomography scan images, including (1) an intact model, (2) a stand-alone (SA) model with no instrument, (3) a lateral rod-screw (LRS) model, (4) a lateral rod-screw plus contralateral translaminar facet screw (LRS-CTLFS) model, (5) a unilateral pedicle screw (UPS) model, and (6) a bilateral pedicle screw (BPS) model. The models of the OLIF cage and pedicle screw were created with a three-dimensional scanning machine to improve the accuracy of the finite element analysis. To analyze the biomechanics of these models, flexion, extension, lateral bending, and 10 N-m moments with a compressive preload of 150 N were imposed on the superior surfaces of the L3 vertebral body. The range of motion (ROM), the stress of the cage, and the stress of fixation were evaluated among the different models. RESULTS: The ROM of the L3-4 and L4-5 vertebra during different physiological motions were similar to the verification criteria reported by Yamamoto et al. The ROM at the L4-5 level of the five operative models decreased for all motion patterns compared with the intact model. The ROM increased from least to greatest as follows: BPS, LRS-CTLFS, UPS, LRS, SA. Differences in the ROM between the BPS and LRS-CTLFS were less than 0.6 degrees and not significant for all loading cases. Compared with the other three models, the stress of the cage was found to be lower in the BPS and LRS-CTLFS under all loading conditions, especially in the BPS. The stress exerted on the fixation was the greatest in the LRS-CTLFS, and the stress experienced by the translaminar facet screw was concentrated in part of the facet joint. CONCLUSIONS: The BPS provided the best biomechanical stability for OLIF, while SA could not provide sufficient stability. The LRS-CTLFS procedure increases the approximate stability and reduces the stress at the cage-endplate interface, except that it causes an increase in screw stress. Clinically, the LRS-CTLFS procedure may reduce many of the traumas and limitations associated with BPS and offer a useful alternative to the BPS procedure during OLIF.

3.
J Foot Ankle Surg ; 59(2): 280-285, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32130991

RESUMO

In this randomized retrospective study, 2 different endoscopic approaches were used to treat intractable plantar fasciitis with the aim to reduce complications and improve therapeutic effects. The lateral double incisions group included 23 feet in 22 patients, and the medial and lateral incisions group included 21 feet in 19 patients. Both groups were treated with endoscopy through the suprafascial approach. Patients were evaluated using the American Orthopaedic Foot and Ankle Society ankle-hindfoot scale (AOFAS-AHS) and visual analog scale (VAS) preoperatively and 3, 6, 12, and 24 months postoperatively. At the final follow-up, the Roles-Maudsley (R-M) score was used to determine patient satisfaction. The AOFAS-AHS scores of the lateral double incisions group were 54.54 ± 7.02 preoperatively and 97.71 ± 3.67 postoperatively. Similarly, AOFAS-AHS scores in the medial and lateral incisions group were 55.52 ± 6.41 preoperatively and 96.64 ± 3.18 postoperatively. There was no significant difference in AOFAS-AHS scores between groups before and after surgery. The time to full weightbearing after surgery and the time to return to full athletic activities in the 2 groups showed no significant difference. The postoperative VAS scores were significantly lower than the preoperative values for both groups. However, no differences were noted in VAS scores or R-M scores postoperatively between the 2 groups. In the medial and lateral incisions group, 3 cases of injury of the first branch of the lateral plantar nerve occurred postoperatively. In conclusion, both endoscopic approaches are effective in the treatment of intractable plantar fasciitis. The lateral double incisions approach showed a lower incidence of nerve injury.

4.
Artigo em Inglês | MEDLINE | ID: mdl-32220495

RESUMO

Many degenerative diseases of the central nervous system (CNS) are associated with demyelination. Oligodendrocyte precursor cells (OPCs) are potential stem cells that can differentiate into oligodendrocytes (OLs) and promote myelination. Promoting the proliferation of OPCs is key to stimulating remyelination and treating neurodegenerative diseases. Herein, we report that astrocytes (ASTs) could increase exosomal secretion of OPCs to promote their proliferation via ITGB4-mediated cell adhesion. Our results demonstrate that ASTs can regulate the proliferation of OPCs through ITGB4-mediated exosomal secretion. OPC proliferation is significantly increased after direct-contact culture with ASTs. Gene ontology (GO) and KEGG pathway analyses reveal that ITGB4/extracellular exosome are closely related to OPC proliferation. siRNA ITGB4 decreases exosomal secretion and OPC proliferation. ITGB4/exosomes remarkably promote OPC transition from G1 to S phase. Furthermore, exosomes can alleviate the inhibitory effect of ITGB4 knockdown on OPC proliferation. Collectively, ASTs regulate OPC exosomal secretion via ITGB4, which could be a valuable approach for promoting OPC proliferation. This strategy may represent a potential treatment for neurological diseases caused by demyelination.

5.
Oncologist ; 25(3): e545-e554, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32162827

RESUMO

BACKGROUND: Human epidermal growth factor receptor 2 (HER2)-mutant lung cancer remains an orphan of specific targeted therapy. The variable responses to anti-HER2 therapies in these patients prompt us to examine impact of HER2 variants and co-mutations on responses to anti-HER2 treatments in lung cancer. PATIENTS AND METHODS: Patients with stage IV/recurrent HER2-mutant lung cancers identified through next-generation sequencings were recruited from seven hospitals. The study comprised a cohort A to establish the patterns of HER2 variants and co-mutations in lung cancer and a cohort B to assess associations between HER2 variants, co-mutations, and clinical outcomes. RESULTS: The study included 118 patients (cohort A, n = 86; cohort B, n = 32). Thirty-one HER2 variants and 35 co-mutations were detected. Predominant variants were A775_G776insYVMA (49/118, 42%), G778_P780dup (11/118, 9%), and G776delinsVC (9/118, 8%). TP53 was the most common co-mutation (61/118, 52%). In cohort B, objective response rates with afatinib were 0% (0/14, 95% confidence interval [CI], 0%-26.8%), 40% (4/10, 14.7%-72.6%), and 13% (1/8, 0.7%-53.3%) in group 1 (A775_G776insYVMA, n = 14), group 2 (G778_P780dup, G776delinsVC, n = 10), and group 3 (missense mutation, n = 8), respectively (p = .018). Median progression-free survival in group 1 (1.2 months; 95% CI, 0-2.4) was shorter than those in group 2 (7.6 months, 4.9-10.4; hazard ratio [HR], 0.009; 95% CI, 0.001-0.079; p < .001) and group 3 (3.6 months, 2.6-4.5; HR, 0.184; 95% CI, 0.062-0.552; p = .003). TP53 co-mutations (6.317; 95% CI, 2.180-18.302; p = .001) and PI3K/AKT/mTOR pathway activations (19.422; 95% CI, 4.098-92.039; p < .001) conferred additional resistance to afatinib. CONCLUSION: G778_P780dup and G776delinsVC derived the greatest benefits from afatinib among HER2 variants. Co-mutation patterns were additional response modifiers. Refining patient population based on patterns of HER2 variants and co-mutations may help improve the efficacy of anti-HER2 treatment in lung cancer. IMPLICATIONS FOR PRACTICE: Human epidermal growth factor receptor 2 (HER2)-mutant lung cancers are a group of heterogenous diseases with up to 31 different variants and 35 concomitant genomic aberrations. Different HER2 variants exhibit divergent sensitivities to anti-HER2 treatments. Certain variants, G778_P780dup and G776delinsVC, derive sustained clinical benefits from afatinib, whereas the predominant variant, A775_G776insYVMA, is resistant to most anti-HER2 treatments. TP53 is the most common co-mutation in HER2-mutant lung cancers. Co-mutations in TP53 and the PI3K/AKT/mTOR pathway confer additional resistance to anti-HER2 treatments in lung cancer. The present data suggest that different HER2 mutations in lung cancer, like its sibling epidermal growth factor receptor, should be analyzed independently in future studies.

6.
Anal Chim Acta ; 1107: 55-62, 2020 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-32200902

RESUMO

Recently, metal-organic frameworks (MOFs) display great application potential in the field of electrochemical catalysis and sensing due to its extraordinary properties. Herein, Co-based MOFs (ZIF-67) decorated graphene nanosheets (GS) heterogeneous hybrids (ZIF-67@GS) with sandwich-like morphology is first prepared by a facile in situ synthesis method. The electrochemical activity of ZIF-67 polyhedrons is effectively enhanced for the introduction of the high conductivity of graphene nanosheets. Subsequently, phytic acid functionalized ZIF-67 with unique core-shell structure decorated GS (PA-ZIF-67@GS) is prepared through the chemical etching effect of phytic acid. Surprisingly, the exposure level of metal active sites, electrochemical active surface area, electron transfer kinetic of the chemically etched ZIF-67@GS are further significantly boosted. Benefiting from the greatly modified interface property, the as-obtained PA-ZIF-67@GS hybrids exhibit excellent electrocatalytic activity toward the oxidation of glucose, and an ultrasensitive nonenzymatic electrochemical sensing platform is then developed. It is believed that this work may provide effective guidance for optimizing the electrochemical catalytic and sensing performance of other series of MOFs.

7.
PLoS One ; 15(3): e0227244, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32196490

RESUMO

Community detection in complex networks is an important issue in network science. Several statistical measures have been proposed and widely applied to detecting the communities in various complex networks. However, due to the lack of flexibility resolution, some of them have to encounter the resolution limit and thus are not compatible with multi-scale structures of complex networks. In this paper, we investigated a statistical measure of interest for community detection, Significance [Sci. Rep. 3 (2013) 2930], and analyzed its critical behaviors based on the theoretical derivation of critical number of communities and the phase diagram in community-partition transition. It was revealed that Significance exhibits far higher resolution than the traditional Modularity when the intra- and inter-link densities of communities are obviously different. Following the critical analysis, we developed a multi-resolution version of Significance for identifying communities in the multi-scale networks. Experimental tests in several typical networks have been performed and confirmed that the generalized Significance can be competent for the multi-scale communities detection. Moreover, it can effectively relax the first- and second-type resolution limits. Finally, we displayed an important potential application of the multi-scale Significance in computational biology: disease-gene identification, showing that extracting information from the perspective of multi-scale module mining is helpful for disease gene prediction.

8.
Artigo em Inglês | MEDLINE | ID: mdl-32061178

RESUMO

Enhancement of cell-matrix adhesion is preferable and crucial in various fields of tissue engineering. Integrins are important receptors that facilitate cell-matrix adhesion, mediated by intracellular molecules and crosstalk with the cadherin adhesion pathway, which mainly facilitates cell-cell adhesion. Protein-tyrosine phosphatase 1B (PTP1B) has emerged as a pivot in the crosstalk between the cadherin adhesion pathway and the integrin adhesion pathway. The phosphorylation state of PTP1B tyrosine-152 (Y152) plays a central role in balancing the two different cell adhesion forms. In this study, a PTP1B Y152 region mimicking (152RM) peptide was designed to decrease the phosphorylation of PTP1B Y152 via competitive inhibition. As a result, the dissociation of cadherin complexes and the release of PTP1B from cadherin had sharply increased, and Src, an important intracellular component of integrin, was activated, indicating that the cadherin adhesion pathway was inhibited, whereas the integrin adhesion pathway was enhanced. Moreover, upon treatment with the 152RM peptide, we observed that the early adhesion of human bone marrow-derived mesenchymal stem cells (MSCs) was accelerated and the anchoring of MSCs on the surface of integrin ligands was enhanced by an enhanced matrix adhesion ability of MSCs themselves. Importantly, the 152RM peptide significantly promoted the adhesion efficiency of MSCs in the selective cell retention technology, which fabricates instant bone implants in clinical settings, to stimulate osteogenesis in vivo.

9.
Med Ultrason ; 22(1): 37-42, 2020 03 01.
Artigo em Inglês | MEDLINE | ID: mdl-32096786

RESUMO

INTRODUCTION: This study aimed to explore the time-effect of color duplex Doppler ultrasound (CDDU) in the diagnosis of vascular erectile dysfunction (ED). MATERIAL AND METHODS: Using a self-control study, we included patients who underwent penile CDDU and cavernosography in our hospital. We compared the arterial peak systolic velocity (PSV) of CDDU among different intervals for the diagnosis of arterial ED. We included 357 patients who were under consideration for vascular ED. RESULTS: We found significant differences in all the pairwise comparison of PSV in the 1st (0-5 min), 2nd (6-10 min), 3rd (11-15 min), and 4th (16-20 min) 4 intervals after the injection of prostaglandin E1 (p<0.001), except the 11-15 min vs. the 16-20 min interval (p=0.387). Using cavernosography, 294 patients were diagnosed with venous ED. Compared with other intervals, the diagnosis of CDDU 11-15 min after the intracavernous injection of 20 ug prostaglandin E1 (PGE1) had the best consistency with cavernosography (Kappa=0.761; p<0.001). Compared with other intervals, CDDU at 11-15 min had the highest specificity (93.65%), the highest Youden index (0.85), positive likelihood ratio of 14.46, positive predictive value of 98.54%, negative predictive value of 71.08% and a coincidence rate of 92.16%. CONCLUSIONS: Our findings support the increased utilization of CDDU for the diagnosis of both arterial and venous ED. The diagnosis at 11-15 min after intracavernous injection of PGE1 is accurate and stable, which would help to simplify the process and shorten the time of CDDU.

10.
Spectrochim Acta A Mol Biomol Spectrosc ; 232: 118154, 2020 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-32106027

RESUMO

In this study, a new fluorescence probe HMAQ based on quinazoline and diaminomaleonitrile was constructed for sensing ClO- and Al3+. A fluorescence blue-shift with 102 nm together with a color change from golden-yellow to colorless was found by hypochlorite-induced hydrolysis of -CH=N- group to release the initial fluorophore. Besides, Al3+ could cause a 72-nm blue-shifted emission spectra and a color change from golden-yellow to brown. As expected, HMAQ exhibited a satisfactory selectivity and sensitivity to ClO-/Al3+ with a quick response. Most notably, the reversibility of the [HMAQ+Al3+] complex could be used to detect ClO- and Al3+ simultaneously without mutual interferences. The detection limits of HMAQ for ClO- and Al3+ were turned out to be 10.2 nM and 1.56 nM, respectively. The high-performance results of real-time detections demonstrated the enormous potential of HMAQ in real-water samples and living cells.

11.
Thorac Cancer ; 2020 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-32077636

RESUMO

Anti-programmed cell death 1 (PD-1) and its ligand (PD-L1) has emerged as a novel immunotherapy for non-small cell lung cancer (NSCLC). However, the proportion of patients who may benefit from immunotherapy is limited and the factors sensitive or resistant to immunotherapy are not completely clear. Therefore, to identify reliable biomarkers as predictors of clinical response and resistance to anti-PD-1/PD-L1 therapies have become increasingly important. Here, we report a case of a patient with bone metastatic NSCLC, who achieved a pathologic complete response after preoperative pembrolizumab treatment. Postoperative pathological examination found no viable cancer cells in the resected pulmonary nodules and lymph nodes. Several high-frequency DNA damage response and repair (DDR) gene mutations including two germline mutations were identified in the primary lesion. Moreover, high PD-L1 expression, Kirsten rat sarcoma viral oncogene homolog (KRAS) combined with tumor protein 53 (TP53) mutations without epidermal growth factor receptor (EGFR)/anaplastic lymphoma kinase (ALK) driver alterations, high infiltration level of CD8-positive cells and M1 macrophages were observed, which were favorable characteristics for immunotherapy. We explored the possible factors related to an excellent response to immune checkpoint inhibitor in this patient and determined that preoperative use of anti-PD-1 therapy might apply to late-stage lung adenocarcinoma patients with multidimensional advantageous biomarkers for treatment with immune checkpoint inhibitors (ICIs). KEY POINTS: We characterized the genomic features and immune microenvironment signature of a lung adenocarcinoma in a patient with bone metastasis who achieved pathologic complete response after pembrolizumab treatment. To evaluate multidimensional advantageous biomarkers for immunotherapy.

12.
Biosens Bioelectron ; 155: 112101, 2020 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-32090873

RESUMO

A novel time-resolved fluorescence blocking lateral flow immunoassay (TRF-BLFIA) was developed for on-site differential diagnosis of pseudorabies virus (PRV)-infected and vaccinated pigs using europium nanoparticles (EuNPs)-labeled virion antigens and high titer PRV gE monoclonal antibodies (PRV gE-mAb). Upon application of a positive serum sample, the specific epitopes of gE protein on the EuNPs-PRV probe were blocked, inhibiting binding to the PRV gE-mAb on the T line, resulting in low or negligible fluorescence signal, whereas when a negative sample was applied, EuNPs-PRV probes would be able to bind the antibody at the T line, leading to high fluorescence signal. Under optimized conditions, TRF-BLFIA provided excellent sensitivity and selectivity. When testing swine clinical samples (n = 356), there was 96.1% agreement between this method and a most widely used commercial gE-ELISA kit. Moreover, our method was rapid (15 min), cost-efficient and easy to operate with simple training, allowing for on-site detection. Thus, TRF-BLFIA could be a practical tool to differentially diagnose PRV-infected and vaccinated pigs.

13.
Curr Alzheimer Res ; 17(1): 93-103, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32053075

RESUMO

BACKGROUND: Studies have suggested that cognitive impairment in Alzheimer's disease (AD) is associated with dendritic spine loss, especially in the hippocampus. Fluoxetine (FLX) has been shown to improve cognition in the early stage of AD and to be associated with diminishing synapse degeneration in the hippocampus. However, little is known about whether FLX affects the pathogenesis of AD in the middle-tolate stage and whether its effects are correlated with the amelioration of hippocampal dendritic dysfunction. Previously, it has been observed that FLX improves the spatial learning ability of middleaged APP/PS1 mice. OBJECTIVE: In the present study, we further characterized the impact of FLX on dendritic spines in the hippocampus of middle-aged APP/PS1 mice. RESULTS: It has been found that the numbers of dendritic spines in dentate gyrus (DG), CA1 and CA2/3 of hippocampus were significantly increased by FLX. Meanwhile, FLX effectively attenuated hyperphosphorylation of tau at Ser396 and elevated protein levels of postsynaptic density 95 (PSD-95) and synapsin-1 (SYN-1) in the hippocampus. CONCLUSION: These results indicated that the enhanced learning ability observed in FLX-treated middle-aged APP/PS1 mice might be associated with remarkable mitigation of hippocampal dendritic spine pathology by FLX and suggested that FLX might be explored as a new strategy for therapy of AD in the middle-to-late stage.

14.
Theranostics ; 10(5): 2229-2242, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32104505

RESUMO

Rationale: The hematopoietic system and skeletal system have a close relationship, and megakaryocytes (MKs) may be involved in maintaining bone homeostasis. However, the exact role and underlying mechanism of MKs in bone formation during steady-state and stress conditions are still unclear. Methods: We first evaluated the bone phenotype with MKs deficiency in bone marrow by using c-Mpl-deficient mice and MKs-conditionally deleted mice. Then, osteoblasts (OBs) proliferation and differentiation and CD31hiEmcnhi tube formation were assessed. The expression of growth factors related to bone formation in MKs was detected by RNA-sequencing and enzyme-linked immunosorbent assays (ELISAs). Mice with specific depletion of TGF-ß1 in MKs were used to further verify the effect of MKs on osteogenesis and angiogenesis. Finally, MKs treatment of irradiation-induced bone injury was tested in a mouse model. Results: We found that MKs deficiency significantly impaired bone formation. Further investigations revealed that MKs could promote OBs proliferation and differentiation, as well as CD31hiEmcnhi vessels formation, by secreting high levels of TGF-ß1. Consistent with these findings, mice with specific depletion of TGF-ß1 in MKs displayed significantly decreased bone mass and strength. Importantly, treatment with MKs or thrombopoietin (TPO) substantially attenuated radioactive bone injury in mice by directly or indirectly increasing the level of TGF-ß1 in bone marrow. MKs-derived TGF-ß1 was also involved in suppressing apoptosis and promoting DNA damage repair in OBs after irradiation exposure. Conclusions: Our findings demonstrate that MKs contribute to bone formation through coupling osteogenesis with angiogenesis by secreting TGF-ß1, which may offer a potential therapeutic strategy for the treatment of irradiation-induced osteoporosis.

15.
16.
BMC Nephrol ; 21(1): 52, 2020 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-32059699

RESUMO

BACKGROUND: Flurbiprofen axetil (FA) is a commonly prescribed agent to relieve perioperative pain, but the relationship between FA and postoperative acute kidney injury (AKI) remains unclear. This study attempted to evaluate the effects of different dose of perioperative FA on postoperative AKI. METHODS: A total of 9915 patients were enrolled for this retrospective study. The clinical characteristics and the prevalence of postoperative AKI among patients non-using, using low dose (50-100 mg), middle dose (100-250 mg) and large dose (≧250 mg) of FA were analyzed respectively. The impact of different dose of FA on postoperative AKI was analyzed using univariable and multivariate logistic regression analysis. RESULTS: The prevalence of postoperative AKI was 6.7% in the overall subjects and 5.1% in 2446 cases who used FA. The incidence of AKI in low dose group was significantly less than that of non use group (4.5% vs 7.2%, P < 0.001), but the incidence of AKI in large dose group was significantly higher than that in the non-use group (18.8% vs 7.2%, P < 0.001). However, there was no significant difference between patients without using FA and subjects using middle dose of FA (7.2% vs 5.6%, p = 0.355). Multivariate logistic regression analysis showed that low dose of FA was a protective factor for postoperative AKI (OR = 0.75, p = 0.0188), and large dose of FA was a risk factor for postoperative AKI (OR = 4.8, p < 0.0001). CONCLUSIONS: The impact of FA on postoperative AKI was dose-dependent, using of low dose FA (50-100 mg) perioperatively may effectively reduce the incidence of postoperative AKI.

17.
Int J Oncol ; 56(3): 697-708, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31922217

RESUMO

Ovarian cancer is the fifth most common type of cancer afflicting women and frequently presents at a late stage with a poor prognosis. While paired box 2 (PAX2) expression is frequently lost in high­grade serous ovarian cancer, it is expressed in a subset of ovarian tumors and may play a role in tumorigenesis. This study investigated the expression of PAX2 in ovarian cancer. The expression of PAX2 in a murine allograft model of ovarian cancer, the RM model, led to a more rapidly growing cell line both in vitro and in vivo. This finding was in accordance with the shorter progression­free survival observed in patients with a higher PAX2 expression, as determined in this study cohort by immunohistochemistry. iTRAQ­based proteomic profiling revealed that proteins involved in fatty acid metabolism and oxidative phosphorylation were found to be upregulated in RM tumors expressing PAX2. The expression of two key fatty acid metabolic genes was also found to be upregulated in PAX2­expressing human ovarian cancer samples. The analysis of existing datasets also indicated that a high expression of key enzymes in fatty acid metabolism was associated with a shorter progression­free survival time in patients with serous ovarian cancer. Thus, on the whole, the findings of this study indicate that PAX2 may promote ovarian cancer progression, involving fatty acid metabolic reprograming.

18.
Org Lett ; 22(3): 1013-1017, 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-31971396

RESUMO

ZnBr2-catalyzed stereoselective [5 + 2] annulation of N-acryloyl indoles with cyclic sulfonyl enamides is reported, providing a concise and efficient synthesis of isoeburnamonine, which is the key intermediate for norvincamine. Both [2 + 2] and [4 + 2] cycloadducts, depending on the ring size of the enamides, have been shown to be the important intermediates for this [5 + 2] annulation.

19.
Sci Total Environ ; 709: 136123, 2020 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-31905557

RESUMO

The situation of eutrophication and shortage of phosphorus resources have triggered the development of new methods for the removal and recovery of phosphorus. In this study, a novel and highly efficient composite (nano-CaO2/BC) was fabricated by using the porous biochar (BC) to load calcium peroxide (CaO2) nanoparticles. The developed nano-CaO2/BC was applied to remove and recover phosphate from P-contained sewage. The phosphate removal performance of the composite was examined using the bulk solutions with different pH values, coexisting anions, composite dosages, and initial phosphate concentrations. The phosphate adsorption was a typical chemisorption process that agreed well with the pseudo-second-order kinetic model. Isotherm studies showed that the adsorption matched well with Langmuir-Freundlich and the maximum adsorption capacity at equilibrium was 213.22 ± 13.57 mg g-1 (298 K). The characterization results demonstrated that the predominant adsorption mechanism was precipitation. Moreover, the composite had good reusability. The seedling growth test confirmed that the P-laden composite can be mixed with soil to promote the growth of seedlings. Therefore, the method of "cycle back to soil" of used composite provided a way of resource utilization and waste disposal.

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