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1.
Artigo em Inglês | MEDLINE | ID: mdl-32866327

RESUMO

Fused-ring electron acceptors have made significant progress in recent years, while the development of fully non-fused ring acceptors has been unsatisfactory. Here, two fully non-fused ring acceptors, o -4TBC-2F and m -4TBC-2F , were designed and synthesized. By regulating the location of the hexyloxy chains, o -4TBC-2F formed planar backbones, while m -4TBC-2F displayed a twisted backbone. Additionally, the o -4TBC-2F film showed a markedly red-shifted absorption after thermal annealing, which indicated the formation of J -aggregates. For fabrication of organic solar cells (OSCs), PBDB-T was used as a donor and blended with the two acceptors. The o -4TBC-2F -based blend films displayed high charge mobilities, lower energy loss and a higher power conversion efficiency (PCE). The optimized devices based on o -4TBC-2F gave a PCE of 10.26%, which was much higher than those based on m -4TBC-2F at 2.63%, and it is one of the highest reported PCE values for fully non-fused ring electron acceptors.

2.
Artigo em Inglês | MEDLINE | ID: mdl-32882889

RESUMO

PURPOSE: The purpose of this study was to investigate Chinese old adults' different body compositions in response to various levels of physical activity (PA). To the best of our knowledge, this is the first study to demonstrate the dose-response relationship between PA and body composition in old adults. METHODS: 2664 participants older than 60 years (males: n = 984, females: n = 1680) were recruited for this cross-sectional health survey. PA was assessed by the short form of the International Physical Activity Questionnaire (IPAQ), and the body composition was measured by bioelectrical impedance analysis (BIA) instruments. The differences of separate body composition indices (lean body mass, LBM; bone mass, BM; and fat mass, FM) of older participants with different PA levels (below PA recommendation and over PA recommendation) were examined using the one-way analysis of variance (ANOVA). To compare the differences of three body composition indices with six different multiples of PA recommendation (0-1 REC, 1-2 REC, 2-4 REC, 4-6 REC, >6 REC), the one-way ANOVA and Turkey's test was used for the post hoc analysis to identify the upper PA-benefit threshold in different indices of body composition. RESULTS: The LBM and BM are significantly higher and the FM are significantly lower in old adults performing more PA volume than the WHO recommendation, compared with individuals performing less PA volume than the WHO recommendation. There were significant increases in LBM for males in "1-2 REC", "2-4 REC", and ">6 REC" groups, compared with the "0-1 REC" group; and there were significant increases in BM for males in "1-2 REC", "2-4 REC", compared with the "0-1 REC" group. The best PA volume for LBM and BM in females was the PA volume of "2-4 REC". Additionally, whether males or females, there was no significant difference in FM between the "0-1 REC" group and other separate groups. CONCLUSION: The PA volume that causes best benefit for body composition of the elderly occurs at 1 to 2 times the recommended minimum PA for males, while it occurs at 2 to 4 times that recommended for females. No additional harms for old adults' body composition occurs at six or more times the recommended minimum PA.

3.
Adv Mater ; : e2002302, 2020 Aug 18.
Artigo em Inglês | MEDLINE | ID: mdl-32812287

RESUMO

Organic solar cells (OSCs) have made rapid progress in terms of their development as a sustainable energy source. However, record-breaking devices have not shown compatibility with large-scale production via solution processing in particular due to the use of halogenated environment-threatening solvents. Here, slot-die fabrication with processing involving hydrocarbon-based solvents is used to realize highly efficient and environmentally friendly OSCs. Highly compatible slot-die coating with roll-to-roll processing using halogenated (chlorobenzene (CB)) and hydrocarbon solvents (1,2,4-trimethylbenzene (TMB) and ortho-xylene (o-XY)) is used to fabricate photoactive films. Controlled solution and substrate temperatures enable similar aggregation states in the solution and similar kinetics processes during film formation. The optimized blend film nanostructures for different solvents in the highly efficient PM6:Y6 blend is adopted to show a similar morphology, which results in device efficiencies of 15.2%, 15.4%, and 15.6% for CB, TMB, and o-XY solvents. This approach is successfully extended to other donor-acceptor combinations to demonstrate the excellent universality of this method. The results combine a method to optimize the aggregation state and film formation kinetics with the fabrication of OSCs with environmentally friendly solvents by slot-die coating, which is a critical finding for the future development of OSCs in terms of their scalable production and high-performance.

4.
Artigo em Inglês | MEDLINE | ID: mdl-32815586

RESUMO

In this work, a record power conversion efficiency of 8.40% was obtained in single-component organic solar cells (SCOSCs) based on double-cable conjugated polymers. This is realized based on the finding that exciton separation plays the same important role as charge transport in SCOSCs. Herein, we designed two double-cable conjugated polymers with almost the identical conjugated backbones and electron-withdrawing side units, but the extra chlorine (Cl) atoms had different positions on the conjugated backbones. We found that, when Cl atoms were positioned at the main chains, the polymer formed the twist backbones, enabling better miscibility with the naphthalene diimide side units. This could improve the interface contact between conjugated backbones and side units, resulting in efficient conversion of excitons into free charges. These observations were confirmed by systematical studies via several advanced measurements. These findings reveal the importance of charge generation process in SCOSCs and also suggest a strategy to improve this process, that is, controlling the miscibility between conjugated backbones and aromatic side units in double-cable conjugated polymers.

5.
Artigo em Inglês | MEDLINE | ID: mdl-32805919

RESUMO

In this work, a ternary blend strategy based on PBDB-T and two small molecular acceptors (IDTT-OB and IDT-PDOT-C6) is demonstrated to simultaneously improve the photocurrent and reduce the voltage loss in organic solar cells (OSCs). The improved photocurrent is partially due to a broad absorption spectrum of the active layer. In addition, we find that the ternary system possesses a higher degree of crystallinity, smaller domain size, higher domain purity, and higher and more balanced charge-carrier mobilities in comparison with the two corresponding binary systems. The reduced voltage loss in the ternary device is mainly due to a lower energy loss (Eloss) of charge carriers. We achieve a Eloss of only 0.50 eV, which is one of the lowest values reported for the ternary nonfullerene OSCs. Our results have demonstrated that all photovoltaic parameters of ternary OSCs can be simultaneously improved by elaborately selecting the three active layer components.

6.
Surg Endosc ; 2020 Aug 03.
Artigo em Inglês | MEDLINE | ID: mdl-32748270

RESUMO

BACKGROUND: The concurrent presence of liver cirrhosis and hepatocellular carcinoma (HCC) poses a challenge for laparoscopic surgeons to establish a routine practice. The aim of this study was to gather evidence and produce recommendations on the safe and effective practice of laparoscopic hepatectomy for patients with solitary HCC (≤ 5 cm) and liver cirrhosis. METHODS: Between October 2013 and October 2014, 356 curative hepatectomies were performed for patients pathologically diagnosed with solitary HCC (≤ 5 cm) accompanied by cirrhosis (stage 4 fibrosis). To overcome selection bias, a 1:2 match using propensity score matching analysis was conducted between laparoscopic and open hepatectomy. Perioperative outcomes were compared between the groups, including hospitalization, operation time, blood loss, and surgical complications. Perioperative inflammation-based markers, including systemic immune-inflammation index (SII), neutrophil-to-lymphocyte ratio (NLR), platelet-to-lymphocyte ratio (PLR), and lymphocyte-to-monocyte ratio (LMR) were collected from medical records and analyzed. RESULTS: There were 43 and 77 patients in the laparoscopic and open groups, respectively. The laparoscopic group had less hepatic inflow occlusion (16.3% vs. 61%; P < 0.001), shorter operation time (155 vs. 170 min; P = 0.004), and shorter postoperative hospital stay (4 vs. 7 days; P < 0.001). Although the difference was not significant (P = 0.154), the rate of postoperative complications tended to be lower in the laparoscopic group (2.3%) compared with the open group (9.1%). The increase in postoperative SII, NLR, and LMR for laparoscopic hepatectomy were significantly lower than for open hepatectomy. NLR < 5.8 on postoperative day 3 was significantly correlated with shorter hospital stay (P < 0.001). CONCLUSIONS: Compared with open hepatectomy, laparoscopic hepatectomy for selected HCC patients, even in the presence of cirrhosis, might result in better perioperative outcomes and postoperative inflammatory response attenuation, and ultimately promote faster recovery. This provides evidence for considering routine laparoscopic hepatectomy through careful selection of patients with HCC.

7.
Brain Res ; 1748: 147071, 2020 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-32827550

RESUMO

GABAA receptors (GABAARs) and glycine receptors (GlyRs) are two principal inhibitory chloride ion channels in the central nervous system. The two receptors do not function independently but cross-talk to each other, i.e., the activation of one receptor would inhibit the other. This cross-talk is present in different patterns across various regions in the central nervous system; however, the factor that determines these patterns is not understood. Here, we show that the pattern of cross-talk between the two receptors is shaped by their relative expression level in a neuron: a higher expression level correlates with louder talk. In line with a tendency of decrease in expression level of GlyRs and increase in expression level of GABAARs from the spinal cord, the brainstem to the neocortex, GlyRs talked much louder (i.e. produced greater inhibition) than GABAARs (one-way pattern) in spinal cord neurons, about equally loud as GABAARs (symmetric pattern) in inferior colliculus neurons and less loud (i.e. less inhibition) than GABAARs (asymmetric pattern) in auditory cortex neurons. Overexpression of GlyRs in inferior colliculus neurons produced an asymmetric pattern that should otherwise have been observed in spinal cord neurons. These expression level-dependent patterns of cross-talk between the two receptors may suggest how the central nervous system uses an alternative mechanism to maintain a delicate level of inhibition through adjusting the proportion of the two receptors in a neuron along its pathway.

8.
Genome Biol ; 21(1): 217, 2020 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-32847609

RESUMO

Germline disease-causing variants are generally more spatially clustered in protein 3-dimensional structures than benign variants. Motivated by this tendency, we develop a fast and powerful protein-structure-based scan (PSCAN) approach for evaluating gene-level associations with complex disease and detecting signal variants. We validate PSCAN's performance on synthetic data and two real data sets for lipid traits and Alzheimer's disease. Our results demonstrate that PSCAN performs competitively with existing gene-level tests while increasing power and identifying more specific signal variant sets. Furthermore, PSCAN enables generation of hypotheses about the molecular basis for the associations in the context of protein structures and functional domains.

9.
J Biomater Appl ; 35(4-5): 544-552, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32660363

RESUMO

Porous titanium scaffolds can provide sufficient mechanical support and bone growth space for large segmental bone defect repair. However, they fail to restore the physiological environment of bone tissue. Barium titanate (BaTiO3) is considered a smart material that can produce an electric field in response to dynamic force. Low-intensity pulsed ultrasound stimulation (LIPUS), as a kind of micromechanical wave, can not only promote bone repair but also induce BaTiO3 to generate an electric field. In our studies, BaTiO3 was coated on porous Ti6Al4V and LIPUS was externally applied to observe the influence of the piezoelectric effect on the repair of large bone defects in vitro and in vivo. The results show that the piezoelectric effect can effectively promote the osteogenic differentiation of bone marrow stromal cells (BMSCs) in vitro as well as bone formation and growth into implants in vivo. This study provides an optional alternative to the conventional porous Ti6Al4V scaffold with enhanced osteogenesis and osseointegration for the repair of large bone defects.

10.
BMC Cancer ; 20(1): 642, 2020 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-32650743

RESUMO

BACKGROUND: The prognosis of patients with combined hepatocellular carcinoma and intrahepatic cholangiocarcinoma (CHC) is usually poor, and effective adjuvant therapy is missing making it important to investigate whether these patients may benefit from adjuvant transarterial chemoembolization (TACE). We aimed to evaluate the efficiency of adjuvant TACE for long-term recurrence and survival after curative resection before and after propensity score matching (PSM) analysis. METHODS: In this retrospective study, of 230 patients who underwent resection for CHC between January 1994 and December 2014, 46 (18.0%) patients received adjuvant TACE. Univariate and multivariate regression analyses were used to identify the independent predictive factors of survival. Cox regression analyses and log-rank tests were used to compare overall survival (OS) and disease-free survival (DFS) between patients who did or did not receive adjuvant TACE. RESULTS: A total of 230 patients (mean age 52.2 ± 11.9 years; 172 men) were enrolled, and 46 (mean age 52.7 ± 11.1 years; 38 men) patients received TACE. Before PSM, in multivariate regression analysis, γ-glutamyl transpeptidase (γ-GT), tumour nodularity, macrovascular invasion (MVI), lymphoid metastasis, and extrahepatic metastasis were associated with OS. Alanine aminotransferase (ALT), MVI, lymphoid metastasis, and preventive TACE (HR: 2.763, 95% CI: 1.769-4.314, p < 0.001) were independent prognostic factors for DFS. PSM created 46 pairs of patients. Before PSM, adjuvant preventive TACE was not associated with an increased risk of OS (HR: 0.911, 95% CI: 0.545-1.520, p = 0.720) or DFS (HR: 3.345, 95% CI: 1.686-6.638, p = 0.001). After PSM, the 5-year OS and DFS rates were comparable in the TACE group and the non-TACE group (OS: 22.7% vs 14.9%, respectively, p = 0.75; DFS: 11.2% vs 14.4%, respectively, p = 0.06). CONCLUSIONS: The present study identified that adjuvant preventive TACE did not influence DFS or OS after curative resection of CHC.

11.
Nutr Metab Cardiovasc Dis ; 30(9): 1500-1511, 2020 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-32620337

RESUMO

BACKGROUND AND AIMS: Consumption of soy foods has been associated with protection against cardiometabolic disease, but the mechanisms are incompletely understood. We hypothesized that habitual soy food consumption associates with gut microbiome composition, metabolite production, and the interaction between diet, microbiota and metabolites. METHODS AND RESULTS: We analyzed dietary soy intake, plasma and stool metabolites, and gut microbiome data from two independent cross-sectional samples of healthy US individuals (N = 75 lean or overweight, and N = 29 obese). Habitual soy intake associated with several circulating metabolites. There was a significant interaction between soy intake and gut microbiome composition, as defined by gut enterotype, on metabolites in plasma and stool. Soy consumption associated with reduced systolic blood pressure, but only in a subset of individuals defined by their gut microbiome enterotype, suggesting that responsiveness to soy may be dependent on microbiome composition. Soy intake was associated with differences in specific microbial taxa, including two taxa mapping to genus Dialister and Prevotella which appeared to be suppressed by high soy intake We identified context-dependent effects of these taxa, where presence of Prevotella was associated with higher blood pressure and a worse cardiometabolic profile, but only in the absence of Dialister. CONCLUSIONS: The gut microbiome is an important intermediate in the interplay between dietary soy intake and systemic metabolism. Consumption of soy foods may shape the microbiome by suppressing specific taxa, and may protect against hypertension only in individuals with soy-responsive microbiota. CLINICAL TRIALS REGISTRY: NCT02010359 at clinicaltrials.gov.

12.
Environ Sci Pollut Res Int ; 27(29): 36913-36919, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32577963

RESUMO

Manure-based fertilizer is usually applied to agricultural soils to increase soil fertility and improve soil quality. However, this practice has an impact on the soil environment, e.g., increasing heavy metal contents. The aim of this study was to evaluate and estimate the accumulation tendencies of eight heavy metals, including arsenic (As), cadmium (Cd), chromium (Cr), copper (Cu), mercury (Hg), lead (Pb), manganese (Mn), and zinc (Zn) in a soil fertilized continuously with dairy manure through a 5 years' field-scale experiment. Contents of the As, Cd, Cr, Cu, Mn, and Zn gradually increased with the fertilization time of dairy manure at the stable rate of around 326 t hm-2 year-1, leading to annual mean increases of 3.6%, 2.4%, 3.9%, 3.8%, 4.2%, and 6.1%, respectively. Based on the prediction of a dynamic mass balance model using the current practice, the contents of Cd and Zn in the fertilized soil would reach the Chinese standard values for agricultural soils in 48 and 35 years. The mitigation measures, such as lower application rates, for the environmental risk of heavy metal accumulation should be considered.


Assuntos
Metais Pesados/análise , Poluentes do Solo/análise , China , Monitoramento Ambiental , Fertilizantes , Esterco/análise , Solo
13.
Nat Commun ; 11(1): 2850, 2020 06 05.
Artigo em Inglês | MEDLINE | ID: mdl-32503972

RESUMO

Integrating association evidence across multiple traits can improve the power of gene discovery and reveal pleiotropy. Most multi-trait analysis methods focus on individual common variants in genome-wide association studies. Here, we introduce multi-trait analysis of rare-variant associations (MTAR), a framework for joint analysis of association summary statistics between multiple rare variants and different traits. MTAR achieves substantial power gain by leveraging the genome-wide genetic correlation measure to inform the degree of gene-level effect heterogeneity across traits. We apply MTAR to rare-variant summary statistics for three lipid traits in the Global Lipids Genetics Consortium. 99 genome-wide significant genes were identified in the single-trait-based tests, and MTAR increases this to 139. Among the 11 novel lipid-associated genes discovered by MTAR, 7 are replicated in an independent UK Biobank GWAS analysis. Our study demonstrates that MTAR is substantially more powerful than single-trait-based tests and highlights the value of MTAR for novel gene discovery.


Assuntos
Biologia Computacional/métodos , Variação Genética , Estudo de Associação Genômica Ampla/métodos , Modelos Genéticos , Herança Multifatorial , Conjuntos de Dados como Assunto , Genoma Humano , Humanos , Metabolismo dos Lipídeos/genética
14.
Adv Mater ; : e2002066, 2020 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-32529680

RESUMO

To take advantages of the intense absorption and fluorescence, high charge mobility, and high dielectric constant of CsPbI3 perovskite quantum dots (PQDs), PQD hybrid nonfullerene organic solar cells (OSCs) are fabricated. Addition of PQDs leads to simultaneous enhancement of open-circuit voltage (VOC ), short-circuit current density (JSC ), and fill factor (FF); power conversion efficiencies are boosted from 11.6% to 13.2% for PTB7-Th:FOIC blend and from 15.4% to 16.6% for PM6:Y6 blend. Incorporation of PQDs dramatically increases the energy of the charge transfer state, resulting in near-zero driving force and improved VOC . Interestingly, at near-zero driving force, the PQD hybrid OSCs show more efficient charge generation than the control device without PQDs, contributing to enhanced JSC , due to the formation of cascade band structure and increased molecular ordering. The strong fluorescence of the PQDs enhances the external quantum efficiency of the electroluminescence of the active layer, which can reduce nonradiative recombination voltage loss. The high dielectric constant of the PQDs screens the Coulombic interactions and reduces charge recombination, which is beneficial for increased FF. This work may open up wide applicability of perovskite quantum dots and an avenue toward high-performance nonfullerene solar cells.

15.
Br J Cancer ; 123(1): 92-100, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32376890

RESUMO

BACKGROUND: Immunoscore have shown a promising prognostic value in many cancers. We aimed to establish and validate an immune classifier to predict survival after curative resection of hepatocellular carcinoma (HCC) patients who have undergone curative resection. METHODS: The immunohistochemistry (IHC) classifier assay was performed on 664 patients with Barcelona Clinic Liver Cancer (BCLC) stage 0 or A HCC. A nine-feature-based HCC-IHC classifier was then constructed by the least absolute shrinkage and selection operator method. The associations between the HCC-IHC classifier and patient outcomes were assessed. Herein, a nomogram was generated from the Cox regression coefficients and evaluated by decision curve analysis. RESULTS: We constructed an HCC-IHC classifier based on nine features; significant differences were found between the low-HCC-IHC classifier patients and high-HCC-IHC classifier patients in the training cohort in the 5-year relapse-free survival rates (46.7% vs. 26.7%, respectively; P < 0.001). The HCC-IHC classifier-based nomogram presented better accuracy than traditional staging systems. CONCLUSIONS: In conclusion, the HCC-IHC classifier could effectively predict recurrence in early-stage HCC patients and supplemented the prognostic value of the BCLC staging system. The HCC-IHC classifier may facilitate patient decision-making and individualise the management of postoperative patients with early-stage HCC.

16.
Nanoscale ; 2020 May 05.
Artigo em Inglês | MEDLINE | ID: mdl-32367099

RESUMO

A trans-dimolybdenum nicotinate (m-Mo2) complex and its isonicotinate isomer (p-Mo2) were synthesized and characterized crystallographically, and their single-molecule charge transport properties were investigated using the STM break junction (STM-BJ) technique. With a quadruply bonded Mo2 complex unit integrated into molecular backbones, the single-molecule conductance for complex molecules was increased by more than one order of magnitude compared with that of the organic π-conjugated analogues 1,4-bis(4-pyridyl)benzene (p-Ph) and 1,4-bis(3-pyridyl)benzene (m-Ph). More interestingly, unlike m-Ph, m-Mo2 with meta connected pyridyl anchors presents larger conductance than that of p-Mo2 with two para connected pyridyl groups. DFT-based transmission calculations revealed that the significant conductance enhancement of Mo2 molecules originates from the largely reduced HOMO-LUMO gap, and the unique d(δ)-p(π) conjugation between the Mo2 unit and the pyridine rings gives rise to a delocalized electronic structure that endows the Mo2 molecules with an unexpected high conductance.

17.
Zhongguo Zhong Yao Za Zhi ; 45(2): 274-284, 2020 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-32237309

RESUMO

In this paper, five representative Chinese herbal decoction pieces of Scutellariae Radix, Paeoniae Radix Alba, vinegar-processed Corydalis Rhizoma, Polygoni Multiflori Radix Praeparata and Lonicerae Japonicae Flos were selected to prepare the corresponding fine powder of pieces, extract powder, semi-extract powder and physical mixed powder. The physical properties of 20 kinds of powders, such as related parameters of particle size, density, stability and flowability, were evaluated comprehensively. The compression curves of powder porosity and tensile strength changing with pressure were plotted, and the Heckel equation and the Kawakita equation were used to describe the powder compression behavior. The results showed that compared with the fine powder of pieces, the compressibility of the semi-extract powder and the extract powder was significantly improved. Compared with the extract powder, the particle size and relative uniformity of the semi-extract powder were increased, indicating that the uniformity of the powder was improved. Besides, the semi-extract powder could reduce the hygroscopicity of the powder. Particularly, the semi-extract powder of Scutellariae Radix, Paeoniae Radix Alba and vinegar-processed Corydalis Rhizoma could maintain the porous structure of the tablet even under a high tableting pressure, which was beneficial to tablet disintegration. For some traditional Chinese medicines(such as Lonicerae Japonicae Flos), the semi-extract powder could reduce the viscosity, which avoided the sticking in the die compression. The semi-extract powder and the physical mixture powder prepared by the same Chinese herbal decoction pieces had similar physical properties and compression behaviors. Principal component analysis(PCA) was carried out on the 17 physical attributes and 5 compression parameters of the powder. It was found that the first principal component mainly reflected the differences among the material sources, while the second principal component could reflect the differences among fine powder of pieces, extract powder, semi-extract powder and physical mixed powder originating from the same Chinese herbal decoction pieces. In this paper, the mechanism of "unification of drugs and excipients" of Chinese medicine semi-extract powder was explained in terms of physical properties and compression behavior of powders, which provided reference for the formulation design and process development of Chinese medicine tablets.


Assuntos
Composição de Medicamentos , Medicamentos de Ervas Chinesas , Excipientes , Medicina Tradicional Chinesa , Extratos Vegetais , Pós , Comprimidos , Tecnologia Farmacêutica
19.
Biochem Pharmacol ; 175: 113921, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32201213

RESUMO

The mammalian target of rapamycin (mTOR) pathway converges diverse environmental cues to support the lung cancer growth and survival. However, the mTOR-targeted mono-therapy does not achieve expected therapeutic effect. Here, we revealed that fangchinoline (FCL), an active alkaloid that purified from the traditional Chinese medicine Stephania tetrandra S. Moore, enhanced the anti-lung cancer effect of mTOR inhibitor everolimus (EVE). The combination of EVE and FCL was effective to activate Notch 3, and subsequently evoked its downstream target c-MYC. The blockage of Notch 3 signal by the molecular inhibitor of γ-secretase or siRNA of Notch 3 reduced the c-MYC expression and attenuated the combinational efficacy of EVE and FCL on cell apoptosis and proliferation. Moreover, the c-MYC could bind to the C/EBP homologous protein (CHOP) promoter and facilitate CHOP transcription. The conditional genetic deletion of CHOP reduced the apoptosis on lung cancer cells to the same degree as blockage of Notch 3/c-MYC axis, providing further evidence for that the Notch 3/c-MYC axis regulates the transcription of CHOP and finally induces apoptosis upon co-treatment of FCL and EVE in lung cancer cells. Overall, our findings, to the best of our knowledge, firstly link CHOP to Notch 3/c-MYC axis-dependent apoptosis and provide the Notch 3/c-MYC/CHOP activation as a promising strategy for mTOR-targeted combination therapy in lung cancer treatment.

20.
Andrologia ; 52(4): e13550, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32149423

RESUMO

China is a sexually conservative country compared with Western countries. To evaluate the psychological characteristics of Chinese erectile dysfunction (ED) patients, we conducted a cross-sectional study of 153 ED outpatients. Patients were interviewed with the Structured Interview on Erectile Dysfunction (SIEDY) for pathogenic quantification. ED was measured by International Index of Erectile Function (IIEF). Depression and anxiety were evaluated with 9-item Patient Health Questionnaire (PHQ-9) and 7-item Generalised Anxiety Disorder Scale (GAD-7) respectively. Most patients (74.5%) were <40 years old. IIEF-5 were significantly correlated with SIEDY scale 3 (r = .16, p = .040) and GAD-7 (p = .15, p = .033). The SIEDY scale 1 increased with age, but the IIEF-5, SIEDY scale 3, PHQ-9 and GAD-7 decreased with age. A negative correlation was observed between ED and psychological stress, which conflicts with many Western-country studies. Younger patients were characterised by milder ED but more psychological stress, while older patients were characterised by worse ED but less psychological stress. Which may be responsible for the conflicting result. Meanwhile, the much younger age distribution among Chinese ED outpatients may indicate that quite a few older ED patients (≥40 years) in China do not seek outpatient service which should merit more attention.

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