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1.
Sci Total Environ ; 834: 155303, 2022 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-35447191

RESUMO

Biodiversity-ecosystem functioning relationships under multiple pressures have recently been the subject of broad studies. For the key primary producer in aquatic ecosystems, phytoplankton, several studies have focused on trait-based functional diversity (FD) and the related functioning (e.g., resource use efficiency, RUE), and their linkages. However, investigations of the effects of environmental factors at different levels (e.g., land use, lake morphometry, climate and nutrients) on FD and RUE are sparse. We developed a data-driven-model framework to simultaneously elucidate the effects of multiple drivers on FD (functional diversity based on dendrograms, FDc and functional richness, FRic) and RUE (of nitrogen and phosphorus) of phytoplankton based on data from 68 Yunnan-Guizhou Plateau lakes, Southwest China. We found that the concentration of total phosphorus, which is mainly affected by land-use intensity and influenced by water depth, was the primary (positive) driver of changes in both FDc and FRic, while RUE was mainly explained by phytoplankton FD (i.e., FRic). These results indicate that water depth and land-use intensity influence indirectly phytoplankton FD and further regulate RUE. Moreover, nonlinear correlations of RUE with FRic were found, which may be caused by interspecific competition and niche differentiation of the phytoplankton community related to nutrient levels. Our finding may help managers to set trade-off targets between FD and RUE in lake ecosystems except for extremely polluted ones, in which the thresholds derived from the Bayesian network, of total phosphorus, total nitrogen and land-use intensity were approximately 0.04 mg/L, 0.50 mg/L and 244 (unitless), respectively. The probability of meeting the RUE objectives was lower in shallow lakes than in deep lakes, but for FRic the opposite was observed.


Assuntos
Lagos , Fitoplâncton , Teorema de Bayes , China , Ecossistema , Nitrogênio/análise , Fósforo/análise , Água
2.
J Dermatol ; 49(6): 661-665, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-35384058

RESUMO

Pulsed-dye laser (PDL), as an effective and frequently-used treatment modality for infantile hemangiomas (IH), could render patients at risk of developing long-term alopecia. Data on alopecia caused by PDL treatment remain scant and the contributing factors are not clear. Our objective was to identify the risk factors associated with long-term alopecia resulting from PDL treatment for scalp IH. We conducted a retrospective study incorporating patients with IH diagnosis and PDL intervention via thoroughly reviewing the clinical database of the dermatology department. Scalp IH patients were further screened and their medical records were collected. Long-term alopecia was defined as no signs of terminal hair regrowth for at least 2 years in this study. Of the 1293 IH patients, 47 (14 boys and 33 girls) with a mean age of 4.5 months (standard deviation, 3.2) were diagnosed as scalp IH and had subsequently undergone PDL treatments. Hair growth in the treatment area of 18 patients (38.3%) nearly returned to normal, 22 patients (46.8%) had varying degrees of hair loss, and seven patients (14.9%) had no hair regrowth (long-term alopecia). Compared with the older patients receiving treatment, IH patients younger than 3 months who started PDL treatment had a higher risk of developing long-term alopecia (odds ratio, 30.833; 95% confidence interval, 4.079-232.025; p = 0.01). The total number of PDL sessions, post-treatment blisters, and location of IH were not shown to be significantly associated with the development of long-term alopecia. Collectively, our study provides an important insight into curating treatments for IH in infants younger than 3 months. PDL treatments for scalp IH may perhaps be avoided or delayed to prevent the development of treatment-associated long-term alopecia.


Assuntos
Hemangioma , Lasers de Corante , Alopecia/etiologia , Feminino , Hemangioma/tratamento farmacológico , Humanos , Lactente , Lasers de Corante/efeitos adversos , Masculino , Estudos Retrospectivos , Fatores de Risco , Couro Cabeludo , Resultado do Tratamento
3.
Magn Reson Med ; 88(2): 871-879, 2022 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-35377480

RESUMO

PURPOSE: To investigate the correlation between DWI, intravoxel incoherent motion (IVIM), and hypoxia-inducible factor 1-alpha (HIF-1α) expression in a nude mouse model of rhabdomyosarcoma based on imaging and pathological comparisons. METHODS: Human rhabdomyosarcoma-derived (RD) cells were inoculated into the right thigh muscle of 20 BALB/c female nude mice. Mice were imaged using 3.0 Tesla MRI system. T1 -weighted imaging, T2 -weighted imaging, DWI, and IVIM images were obtained. ADW4.7 (GE Healthcare, ChicagoAQ34, IL, USA) was used for image processing of ADC, Dslow , Dfast , and f values. All parameter values were independently analyzed by 2 observers. Immunohistochemistry of HIF-1α was performed. We used a specific image-pathology comparison method to ensure correct overlap between the image plane and the pathological section. Mann-Whitney U test or independent sample t test, Pearson or Spearman correlation test, the intragroup correlation coefficient, Kolmogorov-Smirnov test, and receiver operating characteristic curve were used. The correlation between DWI and intravoxel incoherent motion parameter values and HIF-1α expression was determined. RESULTS: There were 10 mice in the low-expression group and 7 in the high-expression group. The ADC and Dslow values were negatively correlated with HIF-1α with correlation coefficients of -0.491 and - 0.702 (P = 0.045 and 0.002). The f value positively correlated with HIF-1α expression (r = 0.485, P = 0.048). ADC, Dslow , and f were significantly different between the high-HIF-1α expression tumors and the low-HIF-1α expression tumors. ADC showed the best predictive performance among all parameters (area under the curve = 0.652, sensitivity = 83.3%, specificity = 63.6%). CONCLUSION: The parameter values of DWI and intravoxel incoherent motion can be used to evaluate the expression of HIF-1α in rhabdomyosarcoma. ADC, Dslow , and f value showed correlation with the expression of HIF-1α.

4.
Macromol Rapid Commun ; : e2200176, 2022 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-35451187

RESUMO

Bacterial infection can delay wound healing, causing wounds to deteriorate and even threaten the patient's life. Recently, although many composite hydrogels as wound dressing have been developed, it is still highly desired to construct photothermal hydrogels with antimicrobial and antioxidant properties to accelerate the infected wound healing. In this work, a hyaluronic acid (HA)-based composite hydrogel consisting of a dopamine-substituted antimicrobial peptide (DAP) and Iron (III) ions is developed, which exhibits photothermal-assisted promotion and acceleration of healing process of bacteria-infected wounds. DAP, serving as both antimicrobial agent and ROS-scavenger, forms Schiff's base bonds with aldehyde hyaluronic acid (AHA) and iron-catechol coordination bonds to reinforce the composite hydrogel. The presence of Fe3+ can also promote covalent polymerization of dopamine, which endows the hydrogel with photothermal capacity. The in vitro and in vivo experiments prove that the composite hydrogel can effectively accelerate the infected wound healing process, including antibacterial, accelerated collagen deposition, and re-epithelization. This study suggests that the multifunctional composite hydrogel possesses remarkable potential for bacteria-infected wound healing by combining inherent antimicrobial activity, antioxidant capability, and photothermal effect.

5.
Curr Med Sci ; 42(2): 434-438, 2022 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-35403954

RESUMO

OBJECTIVE: This study aimed to analyze the fungal species of pathogens isolated from patients with superficial mucocutaneous mycosis from May 2007 to December 2018. METHODS: A retrospective analysis was carried out to determine the pathogenic fungi isolated from patients with superficial fungal infections in the Medical Mycology Clinical Laboratory, Department of Dermatology and Venereology, Union Hospital, from May 2007 to December 2018. RESULTS: A total of 7639 strains were obtained, belonging to 21 genera and 36 species. They mainly consisted of Candida (3707/7639, 48.53%) and dermatophytes (3594/7639, 47.05%). The specimens were skin scales, nail shavings, secretions on the nail grooves, broken or diseased hair and dandruff, secretions or pseudomembrane of the external genitalia, and the oral mucosa. A total of 7300 patients were enrolled in this study, including 3301 males and 3999 females aged 2 months to 92 years old with a median age of 46.04 years old except for 633 patients whose ages were unknown. Two strains of different species were isolated from each of 339 patients at different body sites. The most frequent species were Trichophyton rubrum complex (2906/7639, 38.04%), Candida albicans (2619/7639, 34.28%), and unclassified Candida spp. Dermatophytes were mostly isolated from glabrous skin (2138/3594, 59.49%), with T. rubrum complex being the predominant species. Candida strains were most commonly isolated from mucosal sites (1979/3707, 53.39%), and C. albicans was the most prevalent causative agent. CONCLUSION: The main distribution of pathogenic fungal species isolated from patients with superficial mycosis from 2007 to 2018 in Wuhan, Hubei province and the surrounding areas was that Candida slightly outnumbered dermatophytes. Among all of the isolated strains, T. rubrum complex was the most abundant.


Assuntos
Dermatomicoses , Candida albicans , Dermatomicoses/microbiologia , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Centros de Atenção Terciária
6.
Eur Radiol ; 2022 Mar 12.
Artigo em Inglês | MEDLINE | ID: mdl-35278121

RESUMO

OBJECTIVES: To explore the correlation of parameters derived from intravoxel incoherent motion (IVIM) and diffusion kurtosis imaging (DKI) with Ki-67 labeling index (LI) in soft tissue sarcoma (STS). METHODS: Forty-one patients with STS underwent IVIM and DKI imaging at 3.0 MRI. The standard apparent diffusion coefficient (ADC), true diffusion coefficient (D), pseudo-diffusion coefficient (D*), perfusion fraction (f), mean kurtosis (MK), and mean diffusivity (MD) were compared between Ki-67 low- and high-expression groups by two independent observers. A novel method was used to ensure the topographic correlation of histologic sections and magnetic resonance imaging slices. Receiver operating characteristic (ROC), intraclass correlation coefficient (ICC), and Spearman's rank correlations were performed for statistical analysis. RESULTS: The high-expression group displayed lower standard ADC, D, and MD values and a higher MK value than the low-expression group. No significant differences were found for D∗ and f values. The areas under the curve for standard ADC, D, MD, and MK when discriminating between low- and high-expression groups were 0.736, 0.745, 0.848, and 0.894, respectively. MK was positively correlated with Ki-67 LI (r = 0.809, p < 0.001). Standard ADC, D, and MD were negatively correlated with Ki-67 LI (r = -0.541, -0.556, -0.702, respectively, p < 0.001). CONCLUSIONS: IVIM and DKI parameters are correlated with Ki-67 LI. MK may be the optimal imaging biomarker for assessing the Ki-67 expression of STS. KEY POINTS: • IVIM and DKI parameters are correlated with the expression of Ki-67 in STS. • The MRI-pathology control method ensured a strong correlation between MRI slices and histologic sections, resulting in a robust radiological-pathological correlation.

7.
Clin Rheumatol ; 41(7): 2043-2052, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35230560

RESUMO

OBJECTIVE: To investigate the effect of cyclophosphamide (CYC) on organ involvement and SLE patients' overall and cause-specific mortality. METHODS: Information about CYC prescription was taken from the Jiangsu Lupus database, which was set up to collect medical records from SLE patients since their first admission during 1999-2009 in Jiangsu province, China. Follow-up studies were carried out in 2010 and 2015 to check the survival status of the patients. Cox regression models were used to estimate the hazard ratio (HR) and 95% CI. Kaplan-Meier model was used to assess the effect of CYC on mortality between organ involvement and non-involvement. RESULTS: There were 221 deaths observed out of 2446 SLE patients. CYC users decreased overall mortality of SLE (8.4%) with adjusted HR (95% CI) of 0.74 (0.56-0.97), as compared to non-users. A decrease in overall mortality of SLE was found in the low dosage (< 600 mg) of CYC users, with adjusted HR (95% CI) of 0.54 (0.36-0.81). The protection of CYC on mortality of SLE was further observed in subgroups, such as female; SLEDAI score ≥ 15 group; and those with neuropsychiatric, renal, and hematological involvements, and low serum C3. In addition, CYC could eliminate the differences in mortality between organ involvement and non-involvement, including renal, neuropsychiatric, cardiopulmonary, gastrointestinal, and hematological involvement, but not for mucocutaneous and musculoskeletal involvement. CONCLUSION: Low dosage use of CYC decreased the risk of overall mortality of SLE. CYC might improve the survival of SLE patients with renal, neuropsychiatric, cardiopulmonary, gastrointestinal, and hematological involvements. Key Points • Cyclophosphamide decreases overall mortality of SLE patients. • Decreased mortality is mainly observed from low dosage use of cyclophosphamide. • Cyclophosphamide improves the survival of SLE patients when major systems such as renal, neuropsychiatric, cardiopulmonary, gastrointestinal, and hematological are involved.


Assuntos
Lúpus Eritematoso Sistêmico , Ciclofosfamida/uso terapêutico , Feminino , Seguimentos , Hospitalização , Humanos , Imunossupressores/uso terapêutico , Rim
8.
Int J Hyg Environ Health ; 241: 113932, 2022 04.
Artigo em Inglês | MEDLINE | ID: mdl-35121380

RESUMO

The impact of artificial light at night (ALAN) exposure on health has become increasingly prominent. However, little is known about the effect of ALAN exposure on atopic diseases. In this study, a cross-sectional analysis of incoming students was conducted in 5 geographically disperse universities which locate in Changsha (south), Wuhan (central), Xiamen (east), Urumchi (west), and Hohhot (north), respectively. All incoming students who consented to participate were recruited, followed by a health examination and a questionnaire survey. Prevalent atopic diseases were diagnosed by clinicians. Mean ALAN (nanoWatts/cm2/sr) during their adolescence was obtained from the remote sensing observed nighttime light data matching with their residence information, which was obtained from survey. Mixed generalized linear models (log-binomial) were used to estimate the associations, in terms of prevalence ratio (PR) with 95% confidence interval (CI). A total of 20106 participants were included in the analysis. Based on previous work, we chose factors including socioeconomic status, behavioural factors, major air pollutants, and air climatic parameters for adjustment. After full adjustment, the PR for atopic diseases was 1.35 (95% CI: 1.27-1.42; P < 0.001). The effect size of ALAN was the largest for asthma (PR = 1.80; 95% CI: 1.48-2.19; P < 0.001), followed by atopic rhinitis (PR = 1.42; 95% CI: 1.33-1.51; P < 0.001), and atopic dermatitis (PR = 1.20; 95% CI: 1.06-1.35; P = 0.003). Subgroup analyses by covariates showed consistent results. This study revealed that exposure to ALAN during adolescence may contribute to a higher risk of atopic diseases in young adulthood.


Assuntos
Dermatite Atópica , Eczema , Adolescente , Adulto , Estudos Transversais , Dermatite Atópica/epidemiologia , Humanos , Prevalência , Fatores de Risco , Estudantes , Adulto Jovem
9.
J Biomed Nanotechnol ; 18(1): 277-287, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-35180922

RESUMO

Age-related macular degeneration (AMD) accounts for 8.7% of the global blindness and neovascular form of AMD (nAMD) occupies a large proportion of severe visual loss and legal blindness caused by AMD with a relatively low incidence rate. Choroidal neovascularization (CNV) is overwhelmingly responsible for the occurrence of nAMD as bleeding and fluid leakage followed by abnormal formation of blood vessels could directly lead to loss of central vision so that reduce the choroidal angiogenesis is an ideal treatment method of nAMD. VEGF is an important cytokine which promote the signaling pathway of angiogenesis and the abnormal expression of VEGF is verified in great many CNV cases. Several anti-VEGF drugs have been widely used in clinical treatments such as ranibizumab, bevacizumab and aflibercept. Conbercept, as an originally developed drug in China, has attracted great attention. For the purpose of better treatment efficacy, our group designed a short chain peptide (Sequence: DDIIIRH-NH2, M.W.880.99) for controlled drug release to remedy the drawback of the short half-time period. The peptide could self-assembled into a stable 'hydrogel under pH 7.4 condition and the 3D structure was clearly observed in TEM study. Rheological study exhibited its great injectability so that the hydrogel was a material for intravitreal injection. Statistics exhibited that the hydrogel could release approximately 50% of total conbercept. The In vitro experiments showed that either dose-dependent or the time-dependent incubation with peptide would not decrease the cell viability of HREC, revealing that the peptide was biocompatible. The most important is that co-incubation with HREC obviously reduced the HREC proliferation and tube formation induced by VEGF, ensuring its potential for the treatment efficacy of nAMD.


Assuntos
Hidrogéis , Degeneração Macular , Inibidores da Angiogênese , Humanos , Hidrogéis/uso terapêutico , Injeções Intravítreas , Degeneração Macular/tratamento farmacológico , Ranibizumab/uso terapêutico , Proteínas Recombinantes de Fusão , Fator A de Crescimento do Endotélio Vascular
10.
J Nucl Med ; 2022 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-35115367

RESUMO

18F-FDG PET has limited diagnostic applications in malignant melanoma (MM). 18F-PFPN is a novel PET probe with high affinity and selectivity for melanin. We conducted a clinical study with two aims, firstly to investigate the biodistribution and radiation dosimetry of 18F-PFPN in healthy volunteers, and secondly, to examine the diagnostic utility of 18F-PFPN PET imaging in patients with MM. Methods: 18F-PFPN was synthesized through a fluoro-for-tosyl exchange reaction. Five healthy volunteers were enrolled to investigate the biodistribution, pharmacokinetics, radiation dosimetry, and safety of the tracer. Subsequently, a total of 21 patients with clinically suspected or confirmed MM underwent both 18F-PFPN PET/MR and 18F-FDG PET/CT scans. Normalized maximum standardized uptake values of selected lesions were determined for both tracers and compared in patient- and lesion-based analyses. Results: 18F-PFPN has elevated radiochemical yield and was highly stable in vivo. In healthy volunteers, 18F-PFPN was safe and well-tolerated and its effective absorbed dose was comparable to that of 18F-FDG. In patient-based analysis, 18F-PFPN uptake was higher than 18F-FDG for both primary tumors and nodal metastases. In lesion-based analysis,18F-PFPN PET imaging could detect 365 metastases that were missed on 18F-FDG PET. Additionally, 18F-PFPN PET imaging had clinical value in distinguishing false-positive lesions on 18F-FDG PET. Conclusion: 18F-PFPN is a safe and well-tolerated melanin PET tracer. In a pilot clinical study, 18F-PFPN PET imaging outperformed traditional 18F-FDG PET in identifying both primary MM and its distant spread.

12.
Hum Vaccin Immunother ; 18(1): 2024064, 2022 12 31.
Artigo em Inglês | MEDLINE | ID: mdl-35130110

RESUMO

OBJECTIVES: Vaccinations can prevent COVID-19 and control its spread quickly and efficiently. This study aimed to investigate knowledge and willingness of geriatric care facility staff to receive a COVID-19 vaccine, and to provide a basis for the government to promote the COVID-19 vaccine and guide people to get vaccinated. STUDY DESIGN: A cross-sectional study. METHODS: Data on characteristics of the participants, knowledge about the COVID-19 vaccine, and willingness to get vaccinated for COVID-19 were collected through an online survey from May 19 to June 18, 2021. Statistical analyses were conducted with ANOVA, chi-square, logistic regression. RESULTS: The survey illustrated that the highest score of COVID-19 vaccine knowledge was 50, the lowest 20, and the average 44.22. It also demonstrated that 91.3% of the participants had a good knowledge of COVID-19 vaccine, and that 97.3% participants were willing to receive the COVID-19 vaccine. The results showed that geriatric care facility staff had varied level of the COVID-19 vaccine knowledge depending upon their age, educational background, and other factors, and correspondingly, their willingness to receive COVID-19 vaccine was affected by their knowledge level of COVID-19 vaccine. CONCLUSIONS: In general, the participants, the staff of geriatric care facilities in Anhui Province, had good mastery of the COVID-19 vaccine, and they were willing to get vaccinated. However, there still existed a few participants with poor knowledge, who were unwilling to get vaccinated against COVID-19. Government can guide people to accept vaccination by enhancing publicity about the effects and adverse reactions of COVID-19 vaccines.


Assuntos
Vacinas contra COVID-19 , COVID-19 , Idoso , COVID-19/prevenção & controle , China , Estudos Transversais , Humanos , SARS-CoV-2 , Vacinação
13.
Acta Biomater ; 142: 264-273, 2022 04 01.
Artigo em Inglês | MEDLINE | ID: mdl-35101580

RESUMO

Malignant melanoma remains the life-threatening form of skin cancer with high mortality and poor prognosis. Thus, an ideal melanoma therapeutic strategy is of immediate importance which can remove the primary tumor, as well as inhibit the metastasis and recurrence. Here, we report the fabrication of adjuvant monophosphoryl lipid A (MPLA) lipid bilayer-enveloped and photosensitizer indocyanine green (ICG)-loaded gold nanocages (MLI-AuNCs) for immunogenic phototherapy of aggressive melanoma. Hollow porous AuNCs are used as carriers to deliver MPLA and ICG, and protect ICG from photodegradation. Both AuNCs and ICG absorb near infrared (NIR) light and can be applied in controllable NIR-triggered photothermal and photodynamic combination therapy (PTT/PDT) of melanoma. MLI-AuNCs coated by thermosensitive lipid bilayer exhibit uniform size, good biocompatibility and bioavailability with prominent tumor accumulation, which further improve the PTT/PDT efficacy. MLI-AuNCs under NIR irradiation not only destroy the primary tumor by PTT/PDT, but also elicit robust antitumor immune response with melanoma associated antigens and MPLA released in situ. The released antigens and MPLA subsequently enhance the recruitment and maturation of dendritic cells, which further activate the effector T cells to inhibit metastases and recurrence of melanoma. This immunomodulatory-boosted PTT/PDT nanoplatform provides a new opportunity for highly aggressive melanoma treatment. STATEMENT OF SIGNIFICANCE: An ideal tumor therapeutic strategy not only can remove the primary tumor, but also inhibit metastasis and recurrence. Here, we introduced a versatile nanoplatform MLI-AuNCs for immunogenic phototherapy of aggressive melanoma. Adjuvant MPLA and photosensitizer ICG can be protected and co-delivered to the tumors by thermosensitive lipid-enveloped AuNCs. MLI-AuNCs exhibited prominent tumor accumulation ability and produced the potent PTT/PDT effect to destroy the primary tumors with a single dose of NIR irradiation, as well as elicited the strong antitumor immunity to inhibit the metastasis and relapse. This study may provide a potential therapeutic vaccination strategy against advanced melanoma and other difficult-to-treat cancers.


Assuntos
Melanoma , Nanopartículas , Fotoquimioterapia , Linhagem Celular Tumoral , Ouro/farmacologia , Humanos , Verde de Indocianina/farmacologia , Bicamadas Lipídicas , Melanoma/terapia , Fármacos Fotossensibilizantes/farmacologia , Fototerapia
14.
Small ; 18(8): e2106906, 2022 02.
Artigo em Inglês | MEDLINE | ID: mdl-35199486

RESUMO

Resistive pressure sensors have been widely studied for application in flexible wearable devices due to their outstanding pressure-sensitive characteristics. In addition to the outstanding electrical performance, environmental friendliness, breathability, and wearable comfortability also deserve more attention. Here, a biodegradable, breathable multilayer pressure sensor based piezoresistive effect is presented. This pressure sensor is designed with all biodegradable materials, which show excellent biodegradability and breathability with a three-dimensional porous hierarchical structure. Moreover, due to the multilayer structure, the contact area of the pressure sensitive layers is greatly increased and the loading pressure can be distributed to each layer, so the pressure sensor shows excellent pressure-sensitive characteristics over a wide pressure sensing range (0.03-11.60 kPa) with a high sensitivity (6.33 kPa-1 ). Furthermore, the sensor is used as a human health monitoring equipment to monitor the human physiological signals and main joint movements, as well as be developed to detect different levels of pressure and further integrated into arrays for pressure imaging and a flexible musical keyboard. Considering the simple manufacturing process, the low cost, and the excellent performance, leaf vein-based pressure sensors provide a good concept for environmentally friendly wearable devices.


Assuntos
Dispositivos Eletrônicos Vestíveis , Humanos , Monitorização Fisiológica , Porosidade , Tato
15.
ACS Appl Mater Interfaces ; 14(4): 5856-5866, 2022 Feb 02.
Artigo em Inglês | MEDLINE | ID: mdl-35061361

RESUMO

Rapid and sensitive diagnostics in the early stage of bacterial infection and immediate treatment play critical roles in the control of infectious diseases. However, it remains challenging to develop integrated systems with both rapid detection of bacterial infection and timely on-demand disinfection ability. Herein, we demonstrate a photonic hydrogel platform integrating visual diagnosis and on-site photothermal disinfection by incorporating Fe3O4@C nanoparticles into a poly(hydroxyethyl methacrylate)-co-polyacrylamide (PHEMA-co-PAAm) matrix. In vitro experiments demonstrate that such a hydrogel can respond to pH variation caused by bacterial metabolism and generate the corresponding color changes to realize naked-eye observation. Meanwhile, its excellent photothermal conversion ability enables it to effectively kill bacteria by destroying cell membranes under near-infrared irradiation. Moreover, the pigskin infection wound model also verifies the bacterial detection performance and disinfection ability of the hydrogel in vivo. Our strategy demonstrates a new approach for visual diagnosis and treatment of bacterial infections.


Assuntos
Desinfetantes/uso terapêutico , Hidrogéis/química , Nanopartículas de Magnetita/uso terapêutico , Infecções Cutâneas Estafilocócicas/diagnóstico por imagem , Infecções Cutâneas Estafilocócicas/tratamento farmacológico , Resinas Acrílicas/química , Animais , Desinfetantes/química , Desinfetantes/efeitos da radiação , Escherichia coli/efeitos dos fármacos , Raios Infravermelhos , Nanopartículas de Magnetita/química , Nanopartículas de Magnetita/efeitos da radiação , Camundongos , Células NIH 3T3 , Terapia Fototérmica , Poli-Hidroxietil Metacrilato/química , Staphylococcus aureus/efeitos dos fármacos , Suínos
16.
ACS Nano ; 16(2): 2789-2797, 2022 02 22.
Artigo em Inglês | MEDLINE | ID: mdl-35060692

RESUMO

Tactile sensors with multimode sensing ability are cornerstones of artificial skin for applications in humanoid robotics and smart prosthetics. However, the intuitive and interference-free reading of multiple tactile signals without involving complex algorithms and calculations remains a challenge. Herein a pressure-temperature bimodal tactile sensor without any interference is demonstrated by combining the fundamentally different sensing mechanisms of optics and electronics, enabling the simultaneous and independent sensing of pressure and temperature with the elimination of signal separation algorithms and calculations. The bimodal sensor comprises a mechanoluminescent hybrid of ZnS-CaZnOS and a poly(3,4-ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS) thermoresistant material, endowing the unambiguous transduction of pressure and temperature into optical and electrical signals, respectively. This device exhibits the highest temperature sensitivity of -0.6% °C-1 in the range of 21-60 °C and visual sensing of the applied forces at a low limitation of 2 N. The interference-free and light-emitting characteristics of this device permit user-interactive applications in robotics for encrypted communication as well as temperature and pressure monitoring, along with wireless signal transmission. This work provides an unexplored solution to signal interference of multimodal tactile sensors, which can be extended to other multifunctional sensing devices.


Assuntos
Robótica , Tato , Eletrônica , Próteses e Implantes , Temperatura
17.
Artigo em Inglês | MEDLINE | ID: mdl-35040950

RESUMO

OBJECTIVE: This study was initiated to evaluate mammalian target of rapamycin (mTOR) activation in renal tissue of lupus nephritis (LN) patients. METHODS: This retrospective study included 187 LN patients, 20 diabetic nephropathy (DN) patients, 10 minimal change disease (MCD) patients, and 10 normal controls (NCs). 7 of 187 LN patients had repeated renal biopsies. mTORC1/2 activation was evaluated by immunohistochemistry and multiplexed immunofluorescence. The association of mTORC1/2 activation with the clinicopathologic indices and prognostic outcomes was analysed among 187 LN patients. Proteomics was performed in renal biopsies of 20 LN patients. Proteomics was employed to comprehensively evaluate the impact of mTOR activation on intrarenal gene expression. RESULTS: mTORC1/2 was significantly activated in podocytes, mesangial cells, endothelial cells and tubular epithelial cells of LN patients as compared with those with MCD or NC. The glomerular mTORC1 activation was higher in LN patients compared with DN patients. mTORC1, but not mTORC2, activation strongly correlated with serum albumin, complement C3, proteinuria, and the following pathological biomarkers of LN: crescent formation, interstitial inflammation and fibrosis. Moreover, mTORC1 activation was identified as a prognostic marker in LN patients. Bioinformatic analyses of proteomics and immunohistochemical data unveiled increased complement activation, antigen presentation, and phagocytosis in LN patients with mTORC1 activation. CONCLUSION: Renal mTORC1 activation could be a biomarker to reveal disease activity and predict clinical prognosis in LN patients.

18.
Adv Ther ; 39(1): 583-597, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34816373

RESUMO

INTRODUCTION: Adalimumab has been used successfully in the treatment of psoriasis. The objective of the study is to compare the efficacy, safety, and immunogenicity between HLX03, an adalimumab biosimilar, and adalimumab in Chinese patients with moderate-to-severe plaque psoriasis. METHODS: In this double-blind, active-controlled, parallel-group study, 262 patients with moderate-to-severe plaque psoriasis were randomized (1:1) to receive HLX03 or adalimumab (80 mg at week 1, 40 mg at week 2, and then 40 mg every 2 weeks) for 48 weeks. The primary endpoint was improvement in Psoriasis Area and Severity Index (PASI) score at week 16 comparing to baseline. Equivalence was demonstrated if 95% confidence interval (CI) of the between group difference fell within the equivalence margins of ± 15%. Other efficacy endpoints, safety and immunogenicity were also evaluated. RESULTS: In the full analysis set, PASI improvements at week 16 was 83.5% (n = 131) in the HLX03 group and 82.0% (n = 130) in the adalimumab group, with a least-square-mean difference of 1.5% (95% CI - 3.9% to 6.8%). There were no significant between-group differences in all secondary efficacy analyses including proportion of patients achieving ≥ 75% improvement from baseline PASI (PASI 75), physician global assessment (PGA) 0/1 (clear or almost clear) and change in dermatology life quality index (DLQI) score. The incidences of adverse events and the proportion of patients with antidrug antibodies were also comparable between the two treatment groups. CONCLUSION: HLX03 demonstrated equivalent efficacy, similar safety and immunogenicity to reference adalimumab, supporting its development as an alternative treatment for patients with plaque psoriasis in China. CLINICAL TRIAL REGISTRATION: Chinadrugtrials.org.cn, CTR20171123 (November 27, 2017); ClinicalTrials.gov, NCT03316781 (October 20, 2017).


Plaque psoriasis is a chronic, autoimmune, inflammatory skin disease associated with significant morbidity and reduced quality of life. In China, the prevalence of plaque psoriasis increased four-fold between 1987 and 2012. Adalimumab is a biologic antibody used to treat plaque psoriasis globally. However, high treatment costs remain as a significant barrier to adalimumab therapy. Therefore, HLX03 has been developed as an adalimumab (Humira®) biosimilar, which is almost identical to the licensed reference adalimumab, but less expensive and more accessible to patients. In this randomized clinical trial, the efficacy (ability of a drug to produce the desired treatment effects), safety, and immunogenicity (ability of a drug to induce immune response which would affect its efficacy and safety) of HLX03 were compared with the reference adalimumab in Chinese patients with moderate-to-severe plaque psoriasis. Efficacy was evaluated by comparing the changes in severity and extent of disease using Psoriasis Area and Severity Index score between treatment initiation and week 16. Safety was monitored by adverse events, laboratory tests and vital signs. Immunogenicity was assessed by the incidence of antidrug antibodies. Among the 262 randomized patients, 131 received HLX03 and 130 received adalimumab. Both groups reported similar improvements in Psoriasis Area and Severity Index scores (between-group difference fell within the prespecified equivalence margins), and also in other efficacy evaluations. Additionally, the two treatment groups showed similar safety and immunogenicity profiles. In summary, HLX03 demonstrated equivalent efficacy to adalimumab, validating it as an alternative treatment for patients with plaque psoriasis in China.


Assuntos
Medicamentos Biossimilares , Psoríase , Adalimumab/efeitos adversos , Medicamentos Biossimilares/efeitos adversos , Método Duplo-Cego , Humanos , Psoríase/tratamento farmacológico , Índice de Gravidade de Doença , Resultado do Tratamento
19.
J Magn Reson Imaging ; 55(1): 225-233, 2022 01.
Artigo em Inglês | MEDLINE | ID: mdl-34240504

RESUMO

BACKGROUND: High cellularity and abnormal interstitial structures are some of the unfavorable factors that affect the treatment outcomes and survival of rhabdomyosarcoma (RMS) patients. PURPOSE: To explore the correlation between diffusion-weighted imaging (DWI) and intravoxel incoherent motion (IVIM) with quantitative histopathologic features in a murine model of RMS. STUDY TYPE: Prospective. ANIMAL MODEL: Murine model of RMS (31 female BALB/c nude mice). FIELD STRENGTH/SEQUENCE: 3.0 T; fast spin-echo (FSE) T1-weighted imaging, fast relaxation fast spin-echo (FRFSE) T2-weighted imaging, DWI PROPELLER FSE imaging sequence, and IVIM echo planar imaging sequence; 10 different b-values (0, 50, 100, 150, 200, 400, 600, 800, 1000, and 1200 s/mm2 ). ASSESSMENT: Magnetic resonance imaging (MRI) was performed after 30-45 days of implantation. The following MRI parameters were calculated: apparent diffusion coefficient (ADC), pure diffusion coefficient (D), pseudo-diffusion coefficient (D*), and perfusion fraction (f). Histopathologic features, which contained nuclear, cytoplasmic, and stromal fractions, and the nuclear-to-cytoplasmic ratio within the tumor were measured using image-based segmentation. STATISTICAL TESTS: Pearson's correlation, multiple linear regression analysis, and receiver operating characteristic curve analysis were performed. A P < 0.05 was considered statistically significant. RESULTS: The ADC value showed moderate negative correlation with nuclear fraction (r = -0.540), and moderate positive correlation with stroma fraction (r = 0.474). The D value showed moderate negative correlation with nuclear fraction (r = -0.491), and moderate positive correlation with stroma fraction (r = 0.421). The f value showed a moderate negative correlation with stroma fraction (r = -0.423). The D value showed the best diagnostic ability. The optimal cut-off D value of 0.460 was associated with 77.8% sensitivity and 68.2% specificity (area under the curve, 0.747). DATA CONCLUSION: The ADC, D, and f values obtained from DWI and IVIM images showed moderate correlation with the quantitative histopathologic features in a murine model of RMS. LEVEL OF EVIDENCE: 1 TECHNICAL EFFICACY STAGE: 3.


Assuntos
Imagem de Difusão por Ressonância Magnética , Rabdomiossarcoma , Animais , Modelos Animais de Doenças , Feminino , Humanos , Camundongos , Camundongos Nus , Estudos Prospectivos , Rabdomiossarcoma/diagnóstico por imagem
20.
Curr Probl Cardiol ; 47(6): 100853, 2022 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-34016483

RESUMO

The risk of cardiovascular disease (CVD) in patients with rheumatoid arthritis (RA) is higher than that in patients without RA, and it is even higher than that in patients with diabetes. Autoimmune-mediated inflammation is observed in patients with RA, resulting in endothelial dysfunction, oxidative stress and activation, and vascular migration of white blood cells. Traditionally, RA-associated CVD was assumed to be mediated by disease-related inflammation, resulting in atherosclerosis (AS). However, this concept has been challenged because treatment with anti-rheumatic drugs, such as methotrexate or proinflammatory cytokine antagonists, such as tumor necrosis factor-alpha (TNF-α) inhibitors, did not reduce the risk of CVD in patients with RA. Current cardiovascular guidelines recommend screening and treatment of CVD risk factors in patients with RA but without clear biomarkers and treatment goals. There is no scientific basis for establishing therapeutic targets for cardiovascular risk factors in RA. Numerous studies have shown that the mechanism of early cardiac dysfunction in patients with RA may occur prior to AS. Therefore, it is crucial to explore the related mechanisms to prevent early cardiac dysfunction in patients with RA.

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