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Clin Rheumatol ; 39(8): 2399-2408, 2020 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-32103372


BACKGROUND: Osteoarthritis (OA) is a degenerative chronic illness that most frequently occurs in the knee joint. Daidzein (DZ) an isoflavone has anti-inflammatory and antioxidant activity. The aim of this study was to evaluate the effectiveness of DZ as a treatment for experimental knee OA (KOA) in rats. METHOD: An experimental KOA model was induced by monosodium iodoacetate (MIA) in rats. Thereafter, 49 Wistar albino male rats (250-300 g, 12-16 weeks old) were randomly divided into 7 groups: C (healthy control); DC (KOA + saline); hyaluronic acid (HA); HA+ intraarticular (ia) DZ; oral (po) DZ; ia DZ; HA + po DZ groups. DZ and/or HA were administered intraarticularly to the rats as 50 µL on days 1, 7, 14, and 21. Alternatively, the DZ was administered orally as 0.5 mL twice daily for 21 days. After the treatment, rats were sacrificed by decapitation under general anesthesia. Serum samples were analyzed to determine the total oxidant status (TOS) and total antioxidant status (TAS) and the levels of TNF-α, IL-1ß, MMP-13, and DZ. Knee joint samples underwent histopathological examination, and TNF-α, IL-1ß, NOS2, and MMP-13 were analyzed with immunohistochemical methods. RESULTS: HA, DZ, and DZ + HA effectively reduced the levels of TNF-α, IL-1ß, and MMP-13 in the serum of the DC group (p < 0.001). In groups that received HA, DZ, or DZ + HA, the serum TAS increased compared with the DC group (p < 0.05). When the DZ + HA combination was used, a more pronounced reduction in the levels of TNFα, NOS2, IL-1ß, and MMP-13 was observed in knee joints. In addition, the cracks on the cartilage surface and fibrillation were completely improved in the groups that received HA, DZ, or DZ + HA compared with the DC group. CONCLUSION: DZ had anti-inflammatory and antioxidant effects in a rat OA model. Therefore, DZ, as monotherapy or especially in combination with HA, may be a promising and beneficial therapy for OA. Key Points •DZ has been shown to reduce TNF-α, IL-1ß, and MMP-13 both in serum and in tissue samples taken from the knee-joints. •The cracks on the cartilage surface and fibrillation in KOA were completely improved by using DZ and DZ + HA combination. •DZ may be useful to eliminate/reduce/ameliorate inflammation and oxidative damage in the pathogenesis of KOA. •DZ, alone or in combination with HA, may be a promising natural compound with beneficial effects in the treatment of KOA.

Curr Rheumatol Rev ; 2019 Dec 26.
Artigo em Inglês | MEDLINE | ID: mdl-31878858


OBJECTIVE: Knee osteoarthritis (KOA) is defined as a chronic degenerative joint disease. Obesity is a significant risk factor for KOA. Omentin is an adipose tissue-induced adipokine. The aim of the present study was to investigate the correlation between obesity and serum omentin levels in patients with KOA. METHODS: This study included 60 patients with KOA, 34 obese individuals (O-KOA) and 26 nonobese individuals (NO-KOA) and 40 controls, 17 obese individuals (OC) and 23 nonobese individuals (NOC) matched in terms of age, sex, and body mass index (BMI) who were recruited from the same polyclinic. Blood samples and knee radiographs were obtained from all the subjects, and clinical features, BMI, and laboratory parameters were recorded. The Kellgren-Lawrence (KL) grade and Western Ontario and McMaster Universities Osteoarthritis (WOMAC) index were used to classify the radiographic and clinical findings, respectively. Serum omentin levels were determined using an ELISA. RESULTS: Serum omentin levels in patients were significantly lower than those in the controls (p < 0.05). When the BMI values and KL scores were considered, serum omentin levels significantly decreased in severe O-KOA versus in mild-to-moderate O-KOA. There was no statistically significant decrease in severe NO-KOA versus mild-to-moderate NO-KOA. There was a significant negative correlation between the serum omentin level and BMI and WOMAC index. All findings were supported by a receiver operating characteristic curve analysis. CONCLUSION: Serum omentin levels were inversely related to obesity and the severity of KOA. The data indicate that omentin may be a new biomarker of KOA to our knowledge and may aid the diagnosis of early-stage O-KOA.

Eurasian J Med ; 50(3): 168-172, 2018 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-30515037


Objective: Isoorientin (ISO) is a flavonoid compound extracted from plant species. The goal of this study was to determine the potential antiproliferative effects of ISO in HT-29 human colorectal adenocarcinoma cell line in vitro, specifically on cell viability, apoptosis, and cell cycle pathways. Materials and Methods: The cytotoxic effect of ISO isolated from E. spectabilis was measured using 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay in HT-29 cell lines. Total RNA was isolated using Tri-Reagent protocol. The effects of ISO on apoptosis-related gene were detected using real-time polymerase chain reaction (RT-PCR). The findings were analyzed using "Delta-Delta CT" ΔΔCT method and evaluated using a computer program. Volcano plot analysis was used for comparing groups and the data obtained were statistically analyzed using Student t test. Results: According to XTT result analysis, the 50% inhibitory concentration (IC50) value of ISO was 125 µM at the 48th h in HT-29 cells. The RT-PCR analysis in HT-29 cells showed that Cyclin D1 (CCND1 ), Cyclin-dependent kinase 6 (CDK6), BAX, BCL-2, Checkpoint kinase 1-2 (CHEK1, CHEK2) and Excision repair cross-complementing 1 (ERCC1) expressions were reduced in ISO-treated cells compared with those in the control group of cells. P53, P21, Caspase-3 (CASP-3), Caspase-8 (CASP-8), and Caspase-9 (CASP-9) gene expressions were increased Ataxia Telengiectasia and Rad-3 related (ATR) was activated in the ISO-treated group of cells compared with those in the control group of cells (p<0.05). Conclusion: ISO affected the proliferation of colorectal cancer (CRC) cells via cell cycle pathways. It also altered apoptosis gene expression. These results demonstrated that ISO can be a therapeutic agent for CRC treatment; however, more studies are needed to investigate its mechanism of actions.