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1.
Braz. j. biol ; 83: e246322, 2023. tab, graf
Artigo em Inglês | LILACS-Express | LILACS, VETINDEX | ID: biblio-1285614

RESUMO

Abstract A total of 10 specimens were captured from selected sites of Bajaur Agency FATA, Pakistan using mist nets. The captured specimens were morphologically identified and various morphometric measurements were taken. The head and Body length (HB) of Pipistrellus coromondra and Pipistrellus kuhlii lepidus (n=10) was 43±0.11 mm and 45±1.1 respectively. Morphologically identified Pipistrellus kuhlii confirmed as Pipistrellus kuhlii lepidus based on 16S rRNA sequences. The DNA sequences were submitted to GenBank and accession numbers were obtained (MN 719478 and MT430902). The available 16S rRNA gene sequences of Pipistrellus coromondra and Pipistrellus kuhlii lepidus were retrieved from NCBI and incorporated in N-J tree analysis. Overall, the interspecific genetic variations among Pipistrellus coromondra and Pipistrellus kuhlii lepidus were 8% and 1% respectively. In our recommendation, a comprehensive molecular identification of bats is need of hour to report more cryptic and new species from Pakistan.


Resumo Um total de 10 espécimes foi capturado em locais selecionados da Bajaur Agency FATA, Paquistão, usando redes de neblina. Os espécimes capturados foram identificados morfologicamente e várias medidas morfométricas foram realizadas. O comprimento da cabeça e do corpo (HB) de Pipistrellus coromondra e Pipistrellus kuhlii lepidus (n = 10) foi de 43 ± 0,11 mm e 45 ± 1,1, respectivamente. Pipistrellus kuhlii identificado morfologicamente e confirmado como Pipistrellus kuhlii lepidus com base em sequências de rRNA 16S. As sequências de DNA foram submetidas ao GenBank e os números de acesso foram obtidos (MN 719478 e MT430902). As sequências do gene 16S rRNA disponíveis de Pipistrellus coromondra e Pipistrellus kuhlii lepidus foram recuperadas do NCBI e incorporadas na análise da árvore N-J. No geral, as variações genéticas interespecíficas entre Pipistrellus coromondra e Pipistrellus kuhlii lepidus foram de 8% e 1%, respectivamente. Em nossa recomendação, uma identificação molecular abrangente de morcegos precisa de uma hora para relatar mais espécies crípticas e novas do Paquistão.

2.
Braz J Biol ; 83: e246322, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34431908

RESUMO

A total of 10 specimens were captured from selected sites of Bajaur Agency FATA, Pakistan using mist nets. The captured specimens were morphologically identified and various morphometric measurements were taken. The head and Body length (HB) of Pipistrellus coromondra and Pipistrellus kuhlii lepidus (n=10) was 43±0.11 mm and 45±1.1 respectively. Morphologically identified Pipistrellus kuhlii confirmed as Pipistrellus kuhlii lepidus based on 16S rRNA sequences. The DNA sequences were submitted to GenBank and accession numbers were obtained (MN 719478 and MT430902). The available 16S rRNA gene sequences of Pipistrellus coromondra and Pipistrellus kuhlii lepidus were retrieved from NCBI and incorporated in N-J tree analysis. Overall, the interspecific genetic variations among Pipistrellus coromondra and Pipistrellus kuhlii lepidus were 8% and 1% respectively. In our recommendation, a comprehensive molecular identification of bats is need of hour to report more cryptic and new species from Pakistan.


Assuntos
Quirópteros , Animais , Quirópteros/genética , Paquistão , RNA Ribossômico 16S
4.
8.
Iran J Vet Res ; 17(4): 231-236, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-28224005

RESUMO

Current research highlights the Hspb1 based screening of eight cat populations of the world to investigate the association of newly found locus within cat mammary tumors. Total 180 cats were screened on the basis of Hspb1 4 bp deletion locus (1514-1517del4) which was observed in six mammary tumor cases in Siamese cat breed. Case-control association study revealed the non-significance with P=0.201 and an overall mutant allele frequency of 0.30 ranging from 0.20-0.40 was observed in other cat populations. Similarly, HWE was also obeyed in combined population samples with P=0.860 and found non-significant with range of 0.429-0.708 in other non-Pakistani cat populations as well. These results might be helpful to understand the association of this novel locus in a better way with large sample size of cases and may also serve as a potential marker for mammary tumor diagnosis, particularly in cats and generally in all other animal populations in comparative genetics and genomics context.

9.
Artigo em Inglês | MEDLINE | ID: mdl-26651640

RESUMO

We report here energetic (>100 keV) negative hydrogen ions (H(-)) generated in the interaction of moderately intense (10(18) W cm(-2)) ultrashort laser pulses (45 fs) with transparent hydrogen containing solid targets. An unambiguous and consistent detection of negative hydrogen ions, with a flux of 8×10(11)H(-) ions/sr, has been observed in every single laser shot, using a Thomson parabola ion spectrograph. Simple estimates based on charge transfer cross sections match well with experimental observations. Our method offers the implementation of an intense, ultrashort laser based negative-ion source at a higher repetition rate, which can be important for various applications.

10.
Clin Exp Dermatol ; 40(7): 774-80, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-25703744

RESUMO

BACKGROUND: Oculocutaneous albinism (OCA) is an autosomal recessive disorder of abnormal melanin formation, which results in hypopigmentation of skin, hair and eyes. OCA is classified into four types based on clinical and genetic findings. OCA1 is the most severe form of albinism, and is caused by mutations in the tyrosinase (TYR) gene, while OCA4 is caused due to mutations in SLC45A2. METHODS: In total, 13 families with ≥ 3 members with OCA were enrolled. Family history was ascertained and pedigrees were drawn up. Blood samples were collected and processed for DNA extraction. Linkage analysis was performed by typing three short tandem repeat markers in candidate regions of TYR and SLC45A2. Sequence analysis was performed of all the coding exons and adjacent intronic sequences of both genes. RESULTS: Eight families showed linkage to OCA1 and one family showed linkage to OCA4. Four missense substitutions (p.Arg239Trp, p.Ser192Tyr, p.Ser44Arg and p.Arg77Gln) were identified in TYR in the families with OCA1 linkage, and another missense substitution (p.Gln272Lys) was identified in the family with OCA4 linkage. One of the identified missense substitution (p.Arg77Gln) in TYR was found in five different families, which had a common haplotype. CONCLUSIONS: We identified four missense substitutions in TYR and a single missense substitution in SLC45A2. One missense substitution (p.Arg77Gln) in TYR was found in five different families that originated from the same geographical area and displayed a common haplotype, suggesting a single origin that then spread to different geographical areas of Azad Kashmir, Pakistan.


Assuntos
Albinismo Oculocutâneo/genética , Antígenos de Neoplasias/genética , Grupo com Ancestrais do Continente Asiático/genética , Efeito Fundador , Proteínas de Membrana Transportadoras/genética , Monofenol Mono-Oxigenase/genética , Mutação de Sentido Incorreto , Adolescente , Adulto , Criança , Pré-Escolar , Análise Mutacional de DNA/métodos , Feminino , Ligação Genética , Humanos , Masculino , Paquistão , Linhagem , Adulto Jovem
11.
Artigo em Inglês | MEDLINE | ID: mdl-25215835

RESUMO

We report on the proton acceleration studies from thin metallic foils of varying atomic number (Z) and thicknesses, investigated using a 45 fs, 10 TW Ti:sapphire laser system. An optimum foil thickness was observed for efficient proton acceleration for our laser conditions, dictated by the laser ASE prepulse and hot electron propagation behavior inside the material. The hydrodynamic simulations for ASE prepulse support the experimental observation. The observed maximum proton energy at different thicknesses for a given element is in good agreement with the reported scaling laws. The results with foils of different atomic number Z suggest that a judicious choice of the foil material can enhance the proton acceleration efficiency, resulting into higher proton energy.


Assuntos
Lasers , Prótons , Radiação , Compostos de Alumínio , Óxido de Alumínio , Simulação por Computador , Cobre , Elétrons , Compostos de Ouro , Hidrodinâmica , Níquel , Tantálio , Titânio
12.
Clin Exp Dermatol ; 39(5): 646-8, 2014 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-24934919

RESUMO

The TYR gene (MIM #6069333) is located at position 11q14.3 on the human chromosome, and encodes tyrosinase, which is expressed in melanocytes and controls the biosynthesis of melanin. Most TYR mutations eliminate the activity of tyrosinase, preventing melanocytes from producing any melanin throughout life. People with this form of albinism have white hair, light-coloured eyes and very pale skin. Some mutations in TYR reduce but do not completely eliminate tyrosinase activity, and allow some melanin to be produced. We report a Pakistani family with four members affected by oculocutaneous albinism (OCA). Blood samples were collected from all affected individuals, normal siblings and their parents. Genomic DNA was extracted, and sequence analysis of all the coding exons and adjacent intronic sequences of TYR was performed, which identified a novel missense substitution (p.Ile198Thr). Sequencing of TYR in 90 unrelated healthy individuals showed no sequence variant at this location. Our study expands the mutational spectrum of OCA1.


Assuntos
Albinismo Oculocutâneo/genética , Predisposição Genética para Doença , Monofenol Mono-Oxigenase/genética , Mutação de Sentido Incorreto , Adolescente , Adulto , Grupo com Ancestrais do Continente Asiático/genética , Criança , Pré-Escolar , Análise Mutacional de DNA , Éxons , Feminino , Humanos , Masculino , Linhagem , Adulto Jovem
13.
Langenbecks Arch Surg ; 397(7): 1053-7, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22875223

RESUMO

BACKGROUND: Lymph node ratio (LNR) has been shown to be an independent prognostic factor in stage III colorectal cancer. Abdominoperineal resection (APR) of rectum is historically associated with poorer oncological outcomes compared to other colorectal resections, and significance of LNR in this group of patients has not been studied. OBJECTIVE: Our aim was to determine impact of LNR on oncological outcomes in a series of patients with rectal cancers undergoing APR. PATIENTS AND METHODS: A series of patients who had undergone APR and had lymph node metastasis were identified from a prospectively maintained clinical, histopathological and radiological database. LNR was calculated, and Cox regression was used to determine the impact of factors affecting local recurrence, distal metastases and overall survival. RESULTS: Fifty-eight (42 males) patients were identified to have rectal cancer with lymph node involvement. LNR was an independent predictor of distal metastasis and overall survival at cutoff levels of 0.17, 0.41 and 0.69. CONCLUSION: Lymph node ratio is an independent predictor of survival outcomes in patients with stage III tumours undergoing APR. LNR may help improve stratification of this group of patients.


Assuntos
Neoplasias Colorretais/patologia , Neoplasias Colorretais/cirurgia , Metástase Linfática/patologia , Neoplasias Retais/patologia , Neoplasias Retais/cirurgia , Idoso , Idoso de 80 Anos ou mais , Terapia Combinada , Feminino , Humanos , Excisão de Linfonodo , Masculino , Pessoa de Meia-Idade , Terapia Neoadjuvante , Recidiva Local de Neoplasia , Estadiamento de Neoplasias , Prognóstico , Modelos de Riscos Proporcionais , Reto/patologia , Reto/cirurgia , Sistema de Registros , Estudos Retrospectivos , Fatores de Risco , Taxa de Sobrevida , Resultado do Tratamento
14.
J Coll Physicians Surg Pak ; 21(4): 227-9, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21453620

RESUMO

This study was conducted to determine the safety and efficacy of injectable bulking agents. A total of 13 procedures were performed on 11 patients with faecal incontinence during 2002 to 2007. Patients with internal anal sphincter defect and low incontinence score (Cleveland score < 10) revealed improvement. Patients with higher incontinence score and external sphincter defect secondary to obstetric damage required further intervention. At a median follow-up of 43 months, 7 (63%) patients showed improvement in incontinence score and 4 (32%) showed marked improvement in their symptoms. Fifty six percent of the patients described this as an effective procedure, though the level of effectiveness varied from person to person. Anal injectable collagen was found safe and effective in the management of faecal incontinence. Long-term follow-ups are required to re assess and consider definitive procedure in failed cases.


Assuntos
Colágeno/administração & dosagem , Incontinência Fecal/terapia , Adulto , Idoso , Canal Anal/anormalidades , Canal Anal/diagnóstico por imagem , Incontinência Fecal/diagnóstico por imagem , Feminino , Humanos , Injeções , Masculino , Pessoa de Meia-Idade , Medição da Dor , Estudos Retrospectivos , Ultrassonografia
16.
Tech Coloproctol ; 14(4): 357-8, 2010 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-20683747

RESUMO

Faecal incontinence is a debilitating condition. Sacral neuromodulation may have a role in the treatment of faecal incontinence. We report a case of faecal incontinence secondary to chronic organophosphate poisoning, which was successfully treated with sacral neuromodulation. The patient's faecal incontinence and quality of life improved significantly.


Assuntos
Terapia por Estimulação Elétrica , Incontinência Fecal/induzido quimicamente , Incontinência Fecal/terapia , Plexo Lombossacral/fisiopatologia , Intoxicação por Organofosfatos , Praguicidas/envenenamento , Agricultura , Eletrodos Implantados , Incontinência Fecal/fisiopatologia , Humanos , Masculino , Pessoa de Meia-Idade , Exposição Ocupacional , Qualidade de Vida/psicologia , Resultado do Tratamento
17.
J Ayub Med Coll Abbottabad ; 20(2): 47-50, 2008.
Artigo em Inglês | MEDLINE | ID: mdl-19385457

RESUMO

BACKGROUND: Tuberculosis, being an infectious disease, carries a risk of infection to contacts attending tuberculous patients. This study was conducted to evaluate the risk for household contacts of tuberculous patients as compared to non-contacts. The study was conducted at PGMI, Gulab Devi Hospital and Defence Housing Authority Lahore. The study included 120 household contacts and 80 non-contacts. METHODS: A Cross sectional study for evaluation of antituberculous antibodies levels by ELISA method in two groups; Mantoux positive household contacts 49, Mantoux negative household contacts 71 and normal healthy persons 'non contacts' 80. Routine Haematological investigations like HB, TLC and ESR were done by conventional methods and all the sera of 200 subjects included in the study were tested for IgM, IgG and IgA anti tuberculous antibodies by enzyme linked immunosorbant assay (ELISA). Purified protein derivative 0.1 ml containing 5 TU was injected intradermally. The test was read after 72 hours by measuring the induration around injection site of forearm. RESULTS: There was no difference in the average age of the household contacts and non-contacts. The complaints of pyrexia, night sweats and weight loss were more in house hold contacts as compared to non-contacts. The awareness about BCG vaccination was equal in both. There were 49 contacts with positive Mantoux test while negative Mantoux test was found in 71 contacts. There were only three Mantoux positive among eighty non-contacts. There was no significant difference in the presence of IgM among household contacts as compared to non-contacts. However both IgG and IgA were present in significantly higher number of household contacts compared to non-contacts. CONCLUSION: Household contacts of patients suffering from active pulmonary tuberculosis have more chances of being infected with Mycobacterium tuberculosis as compared to the healthy non-contact, as shown by the higher levels of antituberculous antibodies & positivity of Mantoux test.


Assuntos
Características da Família , Tuberculose Pulmonar/transmissão , Anticorpos Antibacterianos/sangue , Vacina BCG , Estudos Transversais , Ensaio de Imunoadsorção Enzimática , Humanos , Imunoglobulina A/sangue , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Mycobacterium tuberculosis/imunologia , Paquistão/epidemiologia , Vigilância da População , Fatores de Risco , Teste Tuberculínico , Tuberculose Pulmonar/diagnóstico , Tuberculose Pulmonar/epidemiologia
18.
J Laryngol Otol ; 120(6): 463-6, 2006 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-16772054

RESUMO

A gastrostomy feeding tube has become the most acceptable method for long-term feeding support in patients with head and neck cancer. The aim of this study was to compare the complications of percutaneous endoscopically inserted gastrostomy (PEG) tubes, radiologically inserted gastrostomy (RIG) tubes and surgically inserted gastrostomy (open/laparoscopic) (SUR) tubes in head and neck cancer patients and also to compare the mortality rates of these patients. Seventy-eight head and neck cancer patients underwent gastrostomy tube insertion (40 PEG, 28 RIG and 10 SUR) during the period February 2002 to February 2005. There were no significant demographic differences between the three groups. Thirty-six patients (46 per cent) developed complications, 32 minor and four major. All three groups were similar in their rate of minor complications, with the dislodgement and blockage rate being lowest in the PEG group (p > 0.05). The mortality rate was 4 per cent within 30 days of gastrostomy tube insertion. There were no deaths in the PEG group, two deaths in the RIG group and one in the SUR group. The PEG tube was considered superior to the RIG and SUR gastrostomy tubes, had fewer complications and was safer. Thus, PEG tube insertion is our first choice for head and neck cancer patients.


Assuntos
Carcinoma de Células Escamosas/cirurgia , Nutrição Enteral/métodos , Gastrostomia/métodos , Neoplasias de Cabeça e Pescoço/cirurgia , Intubação Gastrointestinal/métodos , Idoso , Feminino , Gastroscopia , Humanos , Intubação Gastrointestinal/efeitos adversos , Masculino , Pessoa de Meia-Idade , Peritonite/etiologia , Pneumonia/etiologia , Complicações Pós-Operatórias , Estudos Retrospectivos , Infecção da Ferida Cirúrgica , Neoplasias da Língua/cirurgia
19.
Int J Gynaecol Obstet ; 87(1): 79-87, 2004 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-15464789

RESUMO

The pilot study in Punjab, Pakistan was one of the five paired demonstration projects sponsored by FIGO in the "Save the Mothers" maternal mortality project. The goal of the project was to bring basic and comprehensive emergency obstetric care (EmOC) to a semiurban and rural area some 30 km from Lahore, where effectively there was none. The aim was to achieve this by using the existing facilities within the rural health system without the deployment of extra specialist staff other than as initial facilitators. This report shows trebling of some performance indicators and an improvement in met need. There is coincidentally a similar increase in the uptake of general medical services. Reducing maternal mortality requires building local capacity for EmOC; the essential components being the premises, trained personnel, equipment, and availability of drugs and blood. Availability and provision of EmOC coupled with changes in the attitude of the population resulted in marked improvement of process indicators.


Assuntos
Mortalidade Materna , Saúde da Mulher , Coleta de Dados , Atenção à Saúde , Feminino , Humanos , Determinação de Necessidades de Cuidados de Saúde , Paquistão , Gravidez , População Rural , Reino Unido
20.
J Pak Med Assoc ; 52(12): 549-53, 2002 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-12627902

RESUMO

OBJECTIVE: To estimate different proteins and immunoglobulins as humoral immune response in patients with intracranial tumours. METHODS: Cellulose acetate membrane electrophoresis for estimation of different proteins and radialimmunodiffusion (RID) for measurement of serum and CSF immunoglobulins were used. RESULTS: Thirty seven patients with different types of tumours showed increase in serum alpha II globulin, significant decrease of IgG, in patients when compared with controls, significant decrease of IgG in malignant tumours compared with benign tumours. CSF albumin, gamma globulin, immunoglobulins G,A,and M were increased. CONCLUSION: Alpha II globulin, steroid therapy and active neoplasia may be responsible for decreased serum immunoglobulins, while raised gamma globulin and immunoglobulins with intact BBB indicates capability of CNS to produce immunoglobulins.


Assuntos
Adenoma/imunologia , Neoplasias Encefálicas/imunologia , Imunoglobulinas/sangue , Imunoglobulinas/líquido cefalorraquidiano , Tumores Neuroectodérmicos/imunologia , Adulto , Barreira Hematoencefálica/imunologia , Proteínas do Líquido Cefalorraquidiano/análise , Feminino , Humanos , Imunodifusão , Masculino
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