Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 20
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Org Lett ; 2020 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-32323547

RESUMO

A metal-free system for regioselective dehydrogenative cross-couplings between indolizines and oxoaldehydes catalyzed by visible light under mild conditions has been described. As an atom economical and eco-friendly protocol, the reaction proceeds in good yields using inexpensive, readily available visible-light sources and the environmentally friendly oxidant oxygen. Various valuable 1,2-dicarbonyl derivatives attached to an indolizine core were easily accessed by the direct dicarbonylation of the sp2 C-H bond.

2.
J Am Chem Soc ; 142(5): 2129-2133, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31955575

RESUMO

Labile heme (LH) is an important signaling molecule in virtually all organisms. However, specifically detecting LH remains an outstanding challenge. Herein, by learning from the bioactivation mechanism of artemisinin, we have developed the first LH-responsive small-molecule fluorescent probe, HNG, based on a 4-amino-1,8-naphthalimide (NG) fluorophore. HNG showed high selectivity for LH without interference from hemin, protein-interacting heme, and zinc protoporphyrin. Using HNG, the changes of LH levels in live cells were imaged, and a positive correlation of LH level with the degree of hemolysis was uncovered in hemolytic mice. Our study not only presents the first molecular probe for specific LH detection but also provides a strategy to construct probes with high specificity through a bioinspired approach.

3.
Chem Commun (Camb) ; 56(13): 1956-1959, 2020 Feb 13.
Artigo em Inglês | MEDLINE | ID: mdl-31956868

RESUMO

Herein, we report a pH stimulus-disaggregated BODIPY sensitizer (PTS) with low background-toxicity for achieving activated photodynamic/photothermal tumor therapy. Both the photodynamic and photothermal properties of PTS can be activated under acidic conditions, and PTS exhibits excellent antitumor properties, which is revealed by both in vitro and in vivo tests.


Assuntos
Compostos de Boro/química , Fármacos Fotossensibilizantes/química , Animais , Compostos de Boro/farmacologia , Compostos de Boro/uso terapêutico , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Humanos , Concentração de Íons de Hidrogênio , Luz , Camundongos , Camundongos Endogâmicos BALB C , Neoplasias/tratamento farmacológico , Fotoquimioterapia , Fármacos Fotossensibilizantes/farmacologia , Fármacos Fotossensibilizantes/uso terapêutico , Fototerapia , Transplante Heterólogo
4.
Nano Lett ; 20(1): 176-183, 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31777250

RESUMO

In chemodynamic therapy (CDT), real-time monitoring of reactive oxygen species (ROS) production is critical to reducing the nonspecific damage during CDT and feasibly evaluating the therapeutic response. However, CDT agents that can emit ROS-related signals are rare. Herein, we synthesize a semiconducting polymer nanoplatform (SPN) that can not only produce highly toxic ROS to kill cancer cells but also emit ROS-correlated chemiluminescent signals. Notably, the efficacy of both chemiluminescence and CDT can be significantly enhanced by hemin doping (∼10-fold enhancement for luminescent intensity). Such ROS-dependent chemiluminescence of SPN allows ROS generation within a tumor to be optically monitored during the CDT process. Importantly, SPN establishes an excellent correlation of chemiluminescence intensities with cancer inhibition rates in vitro and in vivo. Thus, our nanoplatform represents the first intelligent strategy that enables chemiluminescence-imaging-monitored CDT, which holds potential in assessing therapeutic responsivity and predicting treatment outcomes in early stages.

5.
FEBS Open Bio ; 10(1): 18-27, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31515968

RESUMO

Increasing evidence indicates that long noncoding RNAs (lncRNAs) function as important regulators in biological processes and are dysregulated in various tumors. The lncRNA DANCR functions as an oncogene in various cancers, but elucidation of its role in pancreatic cancer (PC) requires further investigation. In the current study, we demonstrate that DANCR was increased in PC tissues and cell lines. Knockdown of DANCR significantly suppressed cell proliferation, migration, and invasion and influenced the levels of epithelial-to-mesenchymal transition-associated proteins, as demonstrated by the observation of enhanced E-cadherin levels and reduced N-cadherin levels in PC cells. In addition, we identified direct binding to the predicted miR-33b binding site on DANCR. We also showed that there is reciprocal repression between DANCR and miR-33b. Furthermore, a miR-33b inhibitor partially abrogated knockdown of DANCR and caused inhibitory effects. We also demonstrated that DANCR functions as a miR-33b sponge to positively regulate MMP16 expression in PC cells. Collectively, the data reveal that DANCR exerts its function by regulating miR-33b/MMP16 expression, implying an important role for a lncRNA-miRNA-mRNA functional network and suggesting a novel potential therapeutic target for PC.

6.
Anal Chem ; 91(23): 15275-15283, 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31674180

RESUMO

Photoacoustic (PA) imaging as a noninvasive biomedical imaging technology exhibits high spatial resolution and deep tissue penetration for in vivo imaging. In order to fully explore the potential of PA imaging in biomedical applications, new contrast agents with improved PA stability and efficiency are in high demand. Herein, we present a new PA agent based on an oxygen-embedded quinoidal nonacene chromophore that is self-assembled into nanoparticles (Nano(O-Nonacene)-PEG), assisted by polyethylene glycol (PEG). Notably, the photothermal conversion efficiency of Nano(O-Nonacene)-PEG is 1.5 fold that of semiconducting polymer nanoparticles (Nano(PCPDTBT)-PEG) and 2.8 fold that of Au nanorods, owing to the low quantum yield of Nano(O-Nonacene)-PEG. Thereby, Nano(O-Nonacene)-PEG possess a greatly elevated PA signal intensity, compared to Nano(PCPDTBT)-PEG and Au nanorods, which have been widely explored for PA imaging. Due to the high resistance to photo bleaching, Nano(O-Nonacene)-PEG exhibits higher PA signal stability, which may be employed for long-term PA imaging. Moreover, when magnetic Zn0.4Fe2.6O4 nanoparticles are incorporated into Nano(O-Nonacene)-PEG, not only are magnetic resonance signals generated but also the photoacoustic efficacy is greatly enhanced. Therefore, Nano(O-Nonacene)-PEG offers distinct properties: (i) the elevated photoacoustic effect allows for high-resolution photoacoustic imaging, (ii) small size (10 nm in diameter) results in efficient tumor-targeting, and (iii) the facile application of efficient photothermal therapy in vivo. The current work offers the possibility of oxygen-embedded quinoidal acene as a promising PA probe for precision phototheranostics.

7.
J Am Chem Soc ; 141(34): 13572-13581, 2019 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-31370392

RESUMO

Cancer treatments are confounded by severe toxic effects toward patients. To address these issues, activatable nanoprobes have been designed for specific imaging and destruction of cancer cells under the stimulation of specific cancer-associated biomarkers. Most activatable nanoprobes were usually activated by some single-factor stimulation, but this restricts therapeutic specificity between diseased and normal tissue; therefore, multifactor activation is highly desired. To this end, we herein develop a novel dual-stimuli responsive theranostic nanoprobe for simultaneously activatable cancer imaging and photothermal therapy under the coactivation of "dual-key" stimulation of "nitric oxide (NO)/acidity", so as to further improve the therapeutic specificity. Specifically, we have integrated a weak electron acceptor (benzo[c][1,2,5]thiadiazole-5,6-diamine) into a donor-π-acceptor-π-donor type chromophore. When the weak acceptor was oxidized by NO in acidic conditions to form a stronger acceptor (5H-[1,2,3]triazolo[4,5-f]-2,1,3-benzothiadiazole), the molecule absorption was significantly increased in the near-infrared region, based on the intramolecular charge transfer (ICT) mechanism. Under the dual-key stimulation of NO/acidity within the tumor associated with inflammation, the nanoprobe can correspondingly output dual signals for ratiometric photoacoustic and photothermal imaging of cancer in vivo and do so with enhanced accuracy and specificity. Our novel nanoprobe exhibited higher photoacoustic signal enhancement under dual-factor activation at 9.8 times that of NO and 132 times that of acidity alone, respectively. Moreover, through such dual activation of NO/acidity, the nanoprobe produces more differentiation of hyperthermia between tumor and normal tissues, to afford satisfactory photothermal therapy with minimal toxic side effects. Thus, our work presents a promising strategy for significantly improving the precision and specificity of cancer imaging and therapy.

9.
J Mol Neurosci ; 69(3): 494-504, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31342266

RESUMO

The occurrence of cerebral infarction commonly takes atherosclerosis as the pathophysiological basis, accompanied by chronic inflammation. Hypersensitive C-reactive protein (hs-CRP) is an important inflammatory factor involved in the formation of atherosclerosis. This study aims to investigate the regulation of hs-CRP expression by long-chain non-coding RNA (LncRNA) MALAT1 in acute cerebral infarction patients. Plasma levels of LncRNA MALAT1 and hsa-miR-145-5p, hsa-miR-140-5p, hsa-miR-483-3p, and hsa-miR-338-3p in 256 Chinese Han ACI patients and 256 controls were analyzed. HUVECs were transfected with LncRNA MALAT1, MALAT1 NC, and si-MALAT1, respectively. The expression levels of hsa-miR-145-5p, hsa-miR-140-5p, hsa-miR-483-3p, and hsa-miR-338-3p were analyzed. Then, HUVECs were transfected with hsa-miR-145-5p inhibitor, hsa-miR-140-5p inhibitor, hsa-miR-483-3p inhibitor, hsa-miR-338-3p inhibitor, and hsa-miR-145-5p mimic, hsa-miR-140-5p mimic, hsa-miR-483-3p mimic, hsa-miR-338-3p mimic, and the expression level of hs-CRP was detected by Western blotting. The levels of hsa-miR-145-5p, hsa-miR-140-5p, hsa-miR-483-3p, and hsa-miR-338-3p in the plasma of ACI patients were significantly lower than those in the control group (p < 0.001), and the plasma LncRNA MALAT1 levels were significantly higher in ACI patients than in the control group (p < 0.001). The level of LncRNA MALAT1 in plasma of ACI patients and control group was negatively correlated with hsa-miR-145-5p, hsa-miR-140-5p, hsa-miR-483-3p, and hsa-miR-338-3p (r = - 0.36, - 0.79, - 0.76, - 0.75; - 0.60, - 0.68, - 0.48, - 0.56). Plasma levels of hsa-miR-145-5p, hsa-miR-140-5p, hsa-miR-483-3p, and hsa-miR-338-3p were negatively correlated with hs-CRP levels in patients with ACI and controls (r = - 0.74, - 0.81, - 0.84, - 0.56; - 0.61, - 0.69, - 0.69, - 0.50). MALAT1 transfection resulted in the decreased levels of hsa-miR-145-5p, hsa-miR-140-5p, hsa-miR-483-3p, and hsa-miR-338-3p in HUVECs while overexpression of hsa-miR-145-5p, hsa-miR-140-5p, hsa-miR-483-3p, and hsa-miR-338-3p led to a decrease in hs-CRP levels in HUVECs. LncRNA MALAT1 induced the upregulation of CRP expression through inhibiting the expression of hsa-miR-145-5p, hsa-miR-140-5p, hsa-miR-483-3p, and hsa-miR-338-3p.

10.
J Musculoskelet Neuronal Interact ; 18(4): 525-529, 2018 12 01.
Artigo em Inglês | MEDLINE | ID: mdl-30511956

RESUMO

OBJECTIVE: This study aims to explore the effects of 25-hydroxyvitamin D on the bone microstructure of rats with type 2 diabetes mellitus (T2MD). METHODS: 40 male Wistar rats were randomly selected for T2MD modeling and injected with streptozotocin solution. The rats in the control group (n=19) were fed with common feed. 25-hydroxyvitamin D was injected into rats with successful modeling results (Treatment group, n=15). The remaining rats were considered as the model group (n=16). The enzyme-linked immunosorbent assay was adopted to determine bone gla protein (BGP) and tartrate-resistant acid phosphatase (TRACP), and an X-ray bone densitometer were applied to observe the vertebral sections. RESULTS: The activity levels of blood glucose, triglyceride, total cholesterol and TRACP in the model group were higher than those in the treatment group and the control group (p⟨0.01), while serum calcium, phosphorus, BGP, ALP, and glycosylated hemoglobin, various indicators of rats in the model group were lower than those in the treatment group and the control group (p⟨0.05). CONCLUSIONS: It is feasible to treat rats with T2MD with 25-hydroxyvitamin D, which can maintain the integrity of bone microstructure and increase the bone health.


Assuntos
Densidade Óssea/efeitos dos fármacos , Diabetes Mellitus Tipo 2/diagnóstico por imagem , Diabetes Mellitus Tipo 2/tratamento farmacológico , Vitamina D/análogos & derivados , Animais , Densidade Óssea/fisiologia , Diabetes Mellitus Tipo 2/sangue , Masculino , Ratos , Ratos Wistar , Resultado do Tratamento , Vitamina D/farmacologia , Vitamina D/uso terapêutico
11.
Chem Sci ; 9(44): 8402-8408, 2018 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-30542589

RESUMO

The complex environment of living organisms significantly challenges the selectivity of classic small-molecule fluorescent probes for bioimaging. Due to their predesigned topological structure and engineered internal pore surface, covalent organic frameworks (COFs) have the ability to filter out coexisting interference components and help to achieve accurate biosensing. Herein, we propose an effective interference-resistant strategy by creating a COF-based hybrid probe that combines the respective advantages of COFs and small-molecule probes. As a proof of concept, a two-photon fluorescent COF nanoprobe, namely TpASH-NPHS, is developed for targeting hydrogen sulfide (H2S) as a model analyte. TpASH-NPHS exhibits limited cytotoxicity, excellent photostability and long-term bioimaging capability. More importantly, compared with the small-molecule probe, TpASH-NPHS achieves accurate detection without the interference from intracellular enzymes. This allows us to monitor the levels of endogenous H2S in a mouse model of cirrhosis.

12.
Chem Sci ; 9(24): 5347-5353, 2018 Jun 28.
Artigo em Inglês | MEDLINE | ID: mdl-30009005

RESUMO

Mitophagy induced by hypoxia plays an important role in regulating cellular homeostasis via the removal of dysfunctional mitochondria in the lysosomal degradation pathway, which results in physiological changes in the mitochondria, such as the pH, polarity and viscosity. However, the lack of an effective method for imaging of both the hypoxic microenvironment and the resulting variable mitochondria limits the visualization of hypoxia-induced mitophagy. Based on the specific mitochondrial pH changes during the hypoxia-induced mitophagy process, we have reported a near-infrared fluorescent probe (NIR-HMA) for real-time simultaneous visualization of the hypoxic microenvironment and the subsequent mitophagy process in live cells. NIR-HMA selectively accumulated in the hypoxic mitochondria in the NIR-MAO form, emitting at 710 nm, and then transformed into NIR-MAOH, emitting at 675 nm, in the acidified mitochondria-containing autolysosomes. Importantly, by smartly tethering the hypoxia-responsive group to the hydroxyl group of the NIR-fluorochrome, which shows ratiometric pH changes, NIR-HMA can differentiate between different levels of the hypoxic microenvironment and mitophagy. Furthermore, using NIR-HMA, we could track the complete mitophagy process from the mitochondria to the autolysosomes and visualize mitophagy caused only by hypoxia both in cancer cells and normal cells. Finally, NIR-HMA was applied to investigate the role that mitophagy plays in the hypoxic microenvironment via the cycling hypoxia-reoxygenation model. We observed a decreased fluorescence ratio after reoxygenation and a further increased mitophagy level after hypoxia was induced again, suggesting that mitophagy might be a self-protective process that allows cells to adapt to hypoxia. Our work may provide an attractive way for real-time visualization of relevant physiological processes in hypoxic microenvironments.

13.
Drug Deliv ; 23(4): 1420-5, 2016 May.
Artigo em Inglês | MEDLINE | ID: mdl-26203688

RESUMO

BACKGROUND: Delivery of anti-cancer drugs into the cancer cells or tissues by multifunctional nanocarriers may provide a new paradigm in cancer treatment. In this study, folate (FA) decorated nanostructured lipid carriers (NLCs) were constructed as nanomedicine for the delivery of curcumin (CUR). METHODS: CUR-loaded NLCs (CUR-NLCs) were prepared. FA containing polyethylene glycol (PEG)-distearoylphosphatidylethanolamine (DSPE) (FA-PEG-DSPE) was synthesized and used for the decoration of CUR-NLCs. Their particle size, zeta potential, and drug encapsulation efficiency (EE) were evaluated. In vitro cytotoxicity study FA decorated CUR-NLCs (FA-CUR-NLCs) was tested in MCF-7 human breast cancer cells (MCF-7 cells). In vivo anti-tumor efficacies of the carriers were evaluated on mice bearing breast cancer model. RESULTS: The optimum FA-CUR-NLCs formulations with the particle size of 127 nm and with a +13 mV surface charge. The growth of MCF-7 cells in vitro was obviously inhibited. FA-CUR-NLCs also displayed the best anti-tumor activity than other formulations in vivo. CONCLUSION: The results demonstrated that FA-CUR-NLCs were efficient in selective delivery to cancer cells over-expressing FA receptors (FRs). Also FA-CUR-NLCs transfer CUR to the breast cancer cells, enhance the anti-tumor capacity. Thus, FA-CUR-NLCs could prove to be a superior nanomedicine to achieve tumor therapeutic efficacy.


Assuntos
Antineoplásicos/administração & dosagem , Antineoplásicos/farmacologia , Neoplasias da Mama/tratamento farmacológico , Curcumina/administração & dosagem , Curcumina/farmacologia , Portadores de Fármacos/química , Sistemas de Liberação de Medicamentos/métodos , Ácido Fólico/química , Lipídeos/química , Nanoestruturas/química , Fosfatidiletanolaminas/química , Polietilenoglicóis/química , Animais , Química Farmacêutica , Feminino , Humanos , Células MCF-7 , Camundongos
14.
Cell Biochem Biophys ; 69(1): 89-92, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24197679

RESUMO

Obestatin, encoded by the same gene as ghrelin, was first described as a physiological opponent of ghrelin. The association between circulating obestatin levels and blood pressure remains unclear. Furthermore, adequate information is non-existent regarding the older male population with hypertension. For this purpose, we enrolled 185 unrelated hypertensive male patients aged ≥ 80 years (range 80-102 years). One hundred seventy nine age-matched healthy subjects served as controls. Plasma levels of obestatin and insulin were measured using commercial ELISA and RIA. HOMA-IR was calculated using standard method. We found that plasma obestatin levels correlated significantly with insulin levels (P = 0.034) and homeostasis model assessment index for insulin resistance (HOMA-IR: P = 0.028). However, plasma obestatin differed non-significantly between hypertensive (5.06 ± 0.68 ng/mL) and non-hypertensive (4.72 ± 0.82 ng/mL) individuals. Plasma obestatin levels were not associated with systolic (P = 0.818) or diastolic (P = 0.564) blood pressure, waist-to-hip ratio (WHR: P = 0.725), uric acid (P = 0.603), total cholesterol (TC: P = 0.589), low-density lipoprotein cholesterol (LDL-C: P = 0.057); high-density lipoprotein cholesterol (HDL-C: P = 0.432), triglyceride (TG: P = 0.418), and fasting blood glucose (FBG: P = 0.101). We, therefore, concluded that fasting circulating obestatin levels did not directly correlate with blood pressure in men aged ≥ 80 years.


Assuntos
Grelina/sangue , Hipertensão/sangue , Insulina/sangue , Idoso de 80 Anos ou mais , Glicemia/metabolismo , Pressão Sanguínea , Estudos de Casos e Controles , HDL-Colesterol/sangue , LDL-Colesterol/sangue , Humanos , Hipertensão/fisiopatologia , Resistência à Insulina , Masculino , Triglicerídeos/sangue , Relação Cintura-Quadril
15.
Zhonghua Yi Xue Za Zhi ; 93(12): 935-8, 2013 Mar 26.
Artigo em Chinês | MEDLINE | ID: mdl-23863681

RESUMO

OBJECTIVE: To explore the effects and mechanisms of Ghrelin on hypertension and insulin resistance in fructose-fed rats. METHODS: A total of 32 male Sprague-Dawley (SD) rats were randomized into control (A) and fructose-fed groups (B). Rats in group B were fed with 10% fructose solution for 4 weeks. Then the rats in group A were randomized into intraperitoneal saline (group GA1) or intraperitoneal 50 nmol/kg Ghrelin (group GA2) and those in group B into intraperitoneal saline (group GB1) or intraperitoneal 50 nmol/kg Ghrelin (group GB2) twice daily for 6 weeks. Caudal arterial pressure was measured weekly. Plasma blood glucose, insulin concentration and lipid profile were measured. And insulin resistance (IR) was calculated by the method of homeostasis model assessment (HOMA). Plasma level of 15-F2t-isoprostane was measured by enzyme-linked immunosorbent assay (ELISA). RESULTS: Ghrelin caused a significant reduction of systolic pressure at Week 3 in group GB2 versus group GB1 (P < 0.05). Maximal effect appeared at Week 5 ((127 ± 5) vs (120 ± 6) mm Hg, P < 0.05, 1 mm Hg = 0.133 kPa), but blood pressure failed to reach normal value. Ghrelin also decreased plasma insulin concentration and HOMA-IR ((9.6 ± 2.6) vs (13.1 ± 3.6) µU/ml, P < 0.05;1.92 ± 0.12 vs 2.78 ± 0.14, P < 0.01). Plasma level of 15-F2t-isoprostane was lower in group GB2 than that in group GB1 ((75 ± 11) vs (102 ± 14) pg/ml, P < 0.01). CONCLUSION: Ghrelin may lower blood pressure, ameliorate insulin resistance and improve insulin sensitivity through inhibiting oxidative stress in fructose-induced rats.


Assuntos
Grelina/farmacologia , Hipertensão/metabolismo , Resistência à Insulina , Animais , Frutose/efeitos adversos , Hipertensão/induzido quimicamente , Hipertensão/fisiopatologia , Masculino , Ratos , Ratos Sprague-Dawley
16.
Intern Med ; 52(13): 1495-7, 2013.
Artigo em Inglês | MEDLINE | ID: mdl-23812197

RESUMO

Clostridium difficile can cause pseudomembranous colitis (PMC). Antimicrobial agent exposure is a risk factor for Clostridium difficile-associated disease, whereas the use of antituberculous (anti-TB) agents is not. We herein report a case of PMC-associated with antituberculous therapy. A 63-year-old woman with tuberculous pericarditis treated with anti-TB agents was admitted for abdominal pain and diarrhea. On colonoscopy, mucoid exudate and yellowish plaque lesions were observed. The anti-TB agents were discontinued, and the patient was treated with metronidazole and clostridium butyricum. Her symptoms were relieved and did not recur when the anti-TB agents were restarted. In this report, we review the literature and discuss the pathogenesis, clinical manifestations, diagnosis and treatment of this case.


Assuntos
Antituberculosos/efeitos adversos , Infecções por Clostridium/diagnóstico , Clostridium difficile , Enterocolite Pseudomembranosa/diagnóstico , Pericardite Tuberculosa/diagnóstico , Infecções por Clostridium/induzido quimicamente , Infecções por Clostridium/complicações , Enterocolite Pseudomembranosa/etiologia , Feminino , Humanos , Pessoa de Meia-Idade , Pericardite Tuberculosa/etiologia
17.
Mitochondrial DNA ; 23(6): 461-5, 2012 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-22917175

RESUMO

Mutations in mitochondrial DNA are associated with cardiovascular diseases. We reported here molecular characterization of a three-generation Han Chinese family with maternally transmitted hypertension. Most strikingly, this family exhibited a high penetrance of hypertension. Sequence analysis of mitochondrial genome showed the presence of 12,338T>C mutation and 12,330A>G mutation and distinct sets of polymorphisms belonging to the Asian haplogroup F2b. Interestingly, the well-known 12,338T>C mutation, which caused a change of methionine in the translational initiation codon of ND5, also localized in two nucleotides adjacent to the 3' end of tRNA(Leu(CUN)), was implied to cause a decrease in ND5 mRNA level as well as to alter tRNA(Leu(CUN)) stability level. Moreover, the highly conserved 12,330A>G mutation, which disrupted the base pairing (6T-67A) in acceptor arm of tRNA(Leu(CUN)), may result in the failure of tRNA(Leu(CUN)) metabolism. Therefore, the combination of ND5 12,338T>C and tRNA(Leu(CUN)) 12,330A>G mutations may contribute to the high penetrance of hypertension in this Chinese family.


Assuntos
Grupo com Ancestrais do Continente Asiático/genética , Hipertensão/genética , Penetrância , Adulto , Sequência de Bases , Análise Mutacional de DNA , DNA Mitocondrial/genética , Família , Feminino , Predisposição Genética para Doença , Humanos , Hipertensão/etnologia , Masculino , Pessoa de Meia-Idade , Dados de Sequência Molecular , Mutação , Linhagem , RNA de Transferência de Leucina/genética , Homologia de Sequência
18.
Cell Biochem Biophys ; 61(2): 377-82, 2011 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-21830127

RESUMO

Thyroid stimulating hormone receptor (TSHR) is thought to play a critical role in the pathogenesis of certain thyroid diseases, including Graves' disease (GD), multinodular thyroid goiter (MTG), and Hashimoto's thyroiditis (HT). In order to understand whether single nucleotide polymorphisms in the TSHR gene contribute to thyroid diseases, we have conducted a case-control study in which, we examined 8 TSHR gene single-nucleotide polymorphisms in introns 1, 4, 5, 6 and exons 7 and 8, respectively, among patients with thyroid diseases. These included one family with GD (3 patients and 9 healthy members); 60 patients with familiar thyroid diseases (30 with GD, 20 with MTG, and 10 with HT patients), 48 sporadic patients with GD and 96 healthy control individuals. Direct sequencing of all 10 exons and part of introns of TSHR gene, in these patients as well as healthy controls revealed eight polymorphisms. A novel polymorphism in exon 8 AGA(Arg) → CGA(Arg). However, there were no significant differences between patients and controls in the incidence of these polymorphisms. These results suggest that the polymorphisms (polymorphism in intron 1 at 81 bp upstream of exon 2; polymorphism in intron 4 at 135 bp upstream of exon 5; polymorphism in intron 4 at 365 bp upstream of exon 5; polymorphism in intron 5 at 69 bp upstream of exon 6; means polymorphism in intron 6 at 13 bp downstream of exon 6; polymorphism in intron 6 at 187 bp upstream of exon 7; E7+16: polymorphism in 16 bp of exon 7; polymorphism in 40 bp of exon 8) of the TSHR gene may not contribute to the pathogenesis of thyroid diseases.


Assuntos
Receptores da Tireotropina/genética , Doenças da Glândula Tireoide/genética , Estudos de Casos e Controles , Éxons/genética , Feminino , Genótipo , Humanos , Íntrons/genética , Masculino , Pessoa de Meia-Idade , Linhagem , Polimorfismo de Nucleotídeo Único/genética
19.
J Huazhong Univ Sci Technolog Med Sci ; 30(3): 307-11, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20556572

RESUMO

The association between fasting plasma ghrelin levels and insulin resistance and blood pressure (BP) in octogenarians was investigated in this study. A total of 487 unrelated octogenarians (including 203 men and 284 women) were enrolled in this cross-sectional study at the Healthy Care Center of Shanghai East Hospital, Tongji University, China, from October 2008 to April 2009. Plasma ghrelin was determined by using the enzyme linked immunosorbent assay (ELISA). Insulin sensitivity was assessed using the homeostasis model of assessment-insulin resistance (HOMA-IR). The age of the participants ranged from 80 to 89 years (mean=83.9+/-4.8 years) with a body mass index (BMI) of 25.3+/-4.9 kg/m2. Plasma ghrelin levels were 20.94+/-5.34 microg/L, being 20.89+/-5.53 microg/L in men and 21.38+/-3.73 microg/L in women respectively. Plasma ghrelin was not associated with systolic (P=0.981) or diastolic (P=0.724) BP, waist circumference (P=0.278), fasting insulin (P=0.246), fasting blood glucose (FBG) (P=0.693) and HOMA-IR (P=0.232). In the control cohort, no significant differences in plasma ghrelin were found between genders (P=0.489), and among subjects with hypertension (BP>140/90 mmHg) (P=0.284) and type 2 diabetes (P=0.776). In conclusion, fasting plasma ghrelin levels are not directly correlated with insulin resistance and BP among octogenarians.


Assuntos
Pressão Sanguínea/fisiologia , Grelina/sangue , Resistência à Insulina , Idoso de 80 Anos ou mais , China , Estudos Transversais , Feminino , Humanos , Masculino
20.
Guang Pu Xue Yu Guang Pu Fen Xi ; 24(2): 173-6, 2004 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-15769009

RESUMO

Three new L-amino acid tailed porphyrins and their zinc(II) complexes were synthesized by L-amino acid and 5-[o-(bromnoethoxyl)phenyl]-10,15,20-triphenyl prophyrin. FABMS, UV-Vis, IR, elemental analysis and chemical analysis were used to determine the structures of these porphyrins and their zinc complexes. The FTIR spectra (4000-400 cm(-1)) of L-amino acid tailed porphyrin and its zinc(II) complexes were measured and investigated. The major bands have been empirically assigned in comparison with L- amino acid tailed porphyrin and its zinc(II) complexes.


Assuntos
Porfirinas/química , Espectrofotometria Infravermelho/métodos , Espectrofotometria Ultravioleta/métodos , Zinco/química , Aminoácidos/química , Eletroquímica/métodos , Porfirinas/síntese química , Espectroscopia de Infravermelho com Transformada de Fourier/métodos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA