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1.
Environ Sci Technol ; 55(8): 4648-4657, 2021 04 20.
Artigo em Inglês | MEDLINE | ID: mdl-33761242

RESUMO

Biodegradable mulch films have been developed as a suitable alternative to conventional nondegradable polyethylene films. However, the key factors controlling the degradation speed of biodegradable mulch films in soils remain unclear. Here, we linked changes in the soil microbiome with the degradation rate of a promising biodegradable material poly(butylene adipate-co-terephthalate) (PBAT) in four soil types, a lou soil (LS), a fluvo-aquic soil (CS), a black soil (BS), and a red soil (RS), equivalent to Inceptisols (the first two soils), Mollisols, and Ultisols, using soil microcosms. The PBAT degradation rate differed with the soil type, with PBAT mineralization levels of 16, 9, 0.3, and 0.9% in LS, CS, BS, and RS, respectively, after 120 days. Metagenomic analysis showed that the microbial community in LS was more responsive to PBAT than the other three soils. PBAT hydrolase genes were significantly enriched in LS but were not significantly stimulated by PBAT in CS, BS, or RS. Several members of Proteobacteria were identified as novel potential degraders, and their enrichment extent was significantly positively correlated with PBAT degradation capacity. Overall, our results suggest that soil environments harbored a range of PBAT-degrading bacteria and the enrichment of potential degraders drives the fate of PBAT in the soils.


Assuntos
Microbiota , Solo , Adipatos , Alcenos , Ácidos Ftálicos , Poliésteres
2.
Polymers (Basel) ; 13(4)2021 Feb 23.
Artigo em Inglês | MEDLINE | ID: mdl-33672202

RESUMO

CO2 convective dissolution has been regarded as one of the fundamental mechanisms to accelerate the mass transfer of CO2 into brine. We present a new spectrophotometric method to characterize the convective instability and measure the dissolved CO2 mass, which enables the real-time quantitative visualization of CO2/brine transport mechanisms. Successive images were captured to identify the finger development regimes, and the convection morphologies were analyzed by the fingers length and affected area. CO2 solubility was experimentally studied, and the results are in agreement with the theoretical calculations. CO2 mass transfer flux was investigated as the Sherwood number changed. The increase in salinity and temperature has a negative effect on CO2 dissolution; here, numerical simulation and experimental phenomena are qualitatively consistent. In general, these findings confirm the feasibility of the method and improve the understanding of the physical process of CO2 convective dissolution, which can help assess the CO2 solubility trapping mass.

3.
Sci Total Environ ; 767: 145473, 2021 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-33636759

RESUMO

Little is known about the response of the soil microbiome (including bacteria in the rhizosphere of legumes such as clover) to mercury (Hg) despite the toxicity of Hg to soil microorganisms. Here, Hg-contaminated soils collected from Guizhou province, southwest China, were divided into three groups according to their Hg contents and were planted with clover. High-throughput sequencing of bacterial 16S rRNA and nitrogenase (nifH) genes and quantitative polymerase chain reaction (qPCR) were used to study the response of bacteria and diazotrophs to soil Hg stress and the effects of Hg on the abundance of functional genes in rhizosphere soils. High concentrations of soil Hg decreased bacterial community abundance and diversity and increased the abundance and diversity of nitrogen-fixing bacteria. LEfSe analysis indicates that Rhizobium was a biomarker at sites with high soil Hg contents and the co-occurrence network results indicate a positive relationship between the abundance of the dominant module (from the co-occurrence network analysis) of Rhizobiaceae and soil Hg concentration. Structural equation modeling (SEM) indicates that the Hg content in the clover shoots (ShootHg) was negatively correlated with the abundance of the mercury reductase (merA) gene (r = -0.26, P < 0.05) and the organomercury lyase (merB) gene (r = -0.23, P < 0.05) in rhizosphere soils. Moreover, correlation analysis and SEM indicate that soil total nitrogen (TN), nitrate­nitrogen (NO3-N), soil organic matter (SOM), and available molybdenum (Mo) contents were also important factors affecting the structure of the microbial community and the abundance of functional genes. The results provide a basis for further study of the mechanism(s) by which microorganisms may impart tolerance of clover to Hg in contaminated soils.


Assuntos
Mercúrio , Microbiota , Poluentes do Solo , China , Medicago , Mercúrio/análise , RNA Ribossômico 16S , Rizosfera , Solo , Microbiologia do Solo , Poluentes do Solo/análise
4.
Wien Klin Wochenschr ; 133(7-8): 321-330, 2021 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-33547492

RESUMO

BACKGROUND: The pandemic of coronavirus disease 2019 (COVID-19) is spreading worldwide with an uncertain ultimate impact on every aspect of human society. Recognizing the groups with higher susceptibility and fatality are in urgent need. METHODS: We retrieved the total number of confirmed incident and death cases of COVID-19 in 177 countries/territories from the Center for Systems Science and Engineering (CSSE) at Johns Hopkins University (JHU). Data of age and gender composition were collected from the United Nations Department of Economic and Social Affairs. Spearman's correlation analysis was used to explore the association between the composition of age and gender and the incidence rate (IRC), case fatality rate (CFRC), and mortality rate of COVID-19 (MRC). Multiple comparisons were adjusted by the Bonferroni method, and the threshold p-value was set as p < 0.01. Software SPSS 23.0, ArcGIS 10.6, and GraphPad Prism 8.0 were used to generate our results. RESULTS: Median age was positively correlated to IRC, CFRC and MRC (rs = 0.60; rs = 0.27; rs = 0.61, p < 0.0001 for all). The age-dependent correlation between people over 65 years of age with IRC was higher in females, while the correlation between age distribution and CFRC as well as MRC was higher in males (p < 0.0001 for all). Besides, we found the age-gender-dependent differences were correlated to IRC in places with high income and associated with CFRC in non-high income countries/territories. CONCLUSION: The correlation between the composition of age and gender and the epidemic characteristics of COVID-19 confirmed previous points that females are more susceptible to COVID-19. The results remind us that more attention should be paid to male patients, particularly those over 65 years old for enhanced clinical management.


Assuntos
Distribuição por Idade , Idoso , Feminino , Humanos , Incidência , Masculino , Pandemias
5.
Environ Sci Technol ; 55(6): 3996-4006, 2021 03 16.
Artigo em Inglês | MEDLINE | ID: mdl-33635627

RESUMO

Perfluoroalkyl substances (PFASs) are highly toxic synthetic chemicals, which are considered the most persistent organic contaminants in the environment. Previous studies have demonstrated that hydrated electron based techniques could completely destruct these compounds. However, in the reactions, alkaline and anaerobic conditions are generally required or surfactants are involved. Herein, we developed a simple binary composite, only including PFAS and hydrated electron source chemical. The system exhibited high efficiency for the utilization of hydrated electrons to decompose PFASs. By comparing the degradation processes of perfluorooctanoic acid (PFOA) in the presence of seven indole derivatives with different chemical properties, we could conclude that the reaction efficiency was dependent on not only the yield of hydrated electrons but also the interaction between PFOA and indole derivative. Among these derivatives, indole showed the highest degradation performance due to its relatively high ability to generate hydrated electrons, and more importantly, indole could form a hydrogen bonding with PFOA to accelerate the electron transfer. Moreover, the novel composite demonstrated high reaction efficiency even with coexisting humic substance and in a wide pH range (4-10). This study would deepen our understanding of the design of hydrated electron based techniques to treat PFAS-containing wastewater.


Assuntos
Fluorcarbonetos , Poluentes Químicos da Água , Elétrons , Substâncias Húmicas , Tensoativos
6.
J Hazard Mater ; 407: 124788, 2021 Apr 05.
Artigo em Inglês | MEDLINE | ID: mdl-33321373

RESUMO

DNA-SIP technology was used to detect active BaP-degraders involved in the biodegradation of benzo [a] pyrene (BaP) in two soils separately and in mixture. The lowest BaP removal was observed in red soil, and Ramlibacter (OTU830) belonging to the γ-Proteobacteria was labeled as BaP degrader with 13C-BaP. The highest diversity of degrading microorganisms occurred in the paddy soil with OTUs belonging to Nocardioids, Micromonospora, Saccharothrix, Lysobacter and Methylium present and a BaP removal efficiency of 29.5% after 14 d. BaP degraders in the mixed microbiome of the soil mixture were Burkholderia and Phenylobacterium, together with Nocardioides and Micromonospora as in the paddy soil. These results indicated that the active BaP-degraders in the mixed microbiome were identical to the active BaP-degraders in paddy soil (OTU356 and OTU328), but also unique in the mixed microbiome, such as OTU393 and OTU392. The functional genes of PAH-ring hydroxylating dioxygenases (PAH-RHDα) were expressed and were positively related to the removal of BaP, and the active BaP degrading bacteria included both Gram-positive and Gram-negative bacteria. Saccharothrix, Phylobacterium, Micromonospora and Nocardioids are here reported as BaP degraders for the first time using DNA-SIP.

7.
Technol Cancer Res Treat ; 19: 1533033820983793, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33357046

RESUMO

BACKGROUND: Cancer stem cells (CSCs) are considered the main cause of cancer recurrence and metastasis, and DNA methylation is involved in the maintenance of CSCs. However, the methylation profile of esophageal CSCs remains unknown. METHODS: Side population (SP) cells were isolated from esophageal squamous cell carcinoma (ESCC) cell lines KYSE150 and EC109. Sphere-forming cells were collected from human primary esophageal cancer cells. SP cells and sphere-forming cells were used as substitutes for cancer stem-like cells. We investigated the genome-wide DNA methylation profile in esophageal cancer stem-like cells using reduced representation bisulfite sequencing (RRBS). RESULTS: Methylated cytosine (mC) was found mostly in CpG dinucleotides, located mostly in the intronic, intergenic, and exonic regions. Forty intersected differentially methylated regions (DMRs) were identified in these 3 groups of samples. Thirteen differentially methylated genes with the same alteration trend were detected; these included OTX1, SPACA1, CD163L1, ST8SIA2, TECR, CADM3, GRM1, LRRK1, CHSY1, PROKR2, LINC00658, LOC100506688, and NKD2. DMRs covering ST8SIA2 and GRM1 were located in exons. These differentially methylated genes were involved in 10 categories of biological processes and 3 cell signaling pathways. CONCLUSIONS: When compared to non-CSCs, cancer stem-like cells have a differential methylation status, which provides an important biological base for understanding esophageal CSCs and developing therapeutic targets for esophageal cancer.

8.
Front Oncol ; 10: 578633, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-33194699

RESUMO

Background: Hepatocellular carcinoma (HCC) with portal vein tumor thrombosis (PVTT) portends a worse prognosis. The objective of this study was to compare the efficacy of percutaneous radiofrequency ablation (RFA) combined with transarterial chemoembolization (TACE) plus sorafenib to that of the most commonly utilized regimen of TACE plus sorafenib in large HCCs with type I/II PVTT. Methods: An open-label, single-center, prospective, randomized trial of participants with tumors ≥5 cm and type I/II PVTT was performed. Participants with previously untreated HCCs were divided into two groups: RFA + cTACE + sorafenib (study group, n = 40) and cTACE + sorafenib (control group, n = 40). The primary endpoint was the objective response rate (ORR), the secondary endpoints included the overall survival (OS); time to progression (TTP); and toxicity. Prognostic factors were analyzed using cox-regression analysis. Results: 80 patients were enrolled into this study with integrated clinical data. Under a median follow-up of 506 days, the median age was 57.5 years (range: 28-80 years). The ORR of study group was higher than control group (70% vs 22.5%, p<0.001). Furthermore, the median OS of study group was superior to that of control group (468 days vs 219 days, HR: 0.44 [95% CI: 0.25-0.78], P = 0.005). Adverse events occurred with 100% probability in both groups (p>0.99), but no treatment-related deaths were recorded. Tumor encapsulation and attaining treatment response predict favorable OS in a multivariate Cox model. The rates of adverse events in both groups were 100% (p>0.99). There were no treatment-related deaths. Conclusions: RFA combined with TACE plus sorafenib is a safe, well-tolerated three-modality treatment for large HCCs with types I/II PVTT, and it demonstrated better efficacy than TACE plus sorafenib alone.

9.
Clin Exp Rheumatol ; 2020 Oct 09.
Artigo em Inglês | MEDLINE | ID: mdl-33124566

RESUMO

OBJECTIVES: The aim of our study was to determine the effect of interleukin (IL)-33 on the proliferation, apoptosis, and secretion of inflammatory cytokines by fibroblast-like synoviocytes (FLSs) in rheumatoid arthritis (RA) and to investigate the underlying mechanisms. METHODS: Cultured RA FLSs and osteoarthritis (OA) FLSs were cocultured with different concentrations of IL-33. TUNEL assay and flow cytometry were used to detect apoptosis. Western blotting and Real-time (RT)-PCR were used to detect the expression levels of B-cell lymphoma 2 (Bcl-2) and Bcl-2-associated X protein (Bax), while the Cell Counting Kit-8 was used to determine cell proliferation in each cocultured group. Enzyme-linked immunosorbent assay was used to detect the expression levels of tumour necrosis factor (TNF)-α and IL-6 in the supernatant from each cell culture. Western blot analysis was used to determine the phosphorylated expression levels of the nuclear factor-kappa light chain enhancer of the activated B cells (NF-κB) pathway in each group. RESULTS: IL-33 inhibited RA FLS apoptosis, promoted FLS proliferation, increased Bcl-2 protein expression levels, and decreased Bax protein expression levels. It also increased the expression levels of inflammatory cytokines TNF-α and IL-6 and increased the expression levels of P-NF-κ B in FLSs. CONCLUSIONS: IL-33 inhibited apoptosis and promoted proliferation of FLSs; in addition, IL-33 increased the serum levels of inflammatory cytokines. The effect of IL-33 on RA FLSs was likely mediated via the NF-κB pathway.

10.
J Gene Med ; 22(12): e3268, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32891070

RESUMO

BACKGROUND: Tau hyperphosphorylation is involved in the progression of Alzheimer's disease (AD). In the present study, we aimed to evaluate the role of linc00507 with respect to modulating Tau phosphorylation in ab AD animal and an Aß42-SH-SY5Y cell model. METHODS: Aß precursor protein (APP)/PS transgenic mice and Aß42-SH-SY5Y cell model were used to investigate the role of linc00507 in AD. A quantitative real-time polymerase chain reaction evaluated the RNA expression of linc00507, miR-181c-5p and microtubule-associated protein tau (MAPT)/tau-tubulin kinase-1 (TTBK1). The interactions between the genes were investigated through changes in one gene expression by regulating another gene in cells and, in addition, correlation assays were performed in mice. Western blot assays examined the protein expression of MAPT/TTBK1, phosphorylation of tau and signaling proteins P25/P35/GSK3ß in response to the regulation of linc00507, miR-181c-5p and MAPT/TTBK1 in cells and also in mice. RESULTS: linc00507 was significantly elevated in hippocampus, and cerebral cortex of APP/PS transgenic mice and AD-like SH-SY5Y cells. It could bind miR-181c-5p and thereby regulate the expression of microtubule-associated protein Tau (MAPT) and tau-tubulin kinase-1 (TTBK1) as a competitive endogenous RNA (ceRNA). MAPT (encoding the tau protein) and TTBK1 (encoding a tau kinase) were identified as direct target genes of miR-181c-5p. Furthermore, linc00507 mediated tau protein hyperphosphorylation by the activation of the P25/P35/GSK3ß signaling pathway through regulating MAPT/TTBK1 by sponging miR-181c-5p. CONCLUSIONS: The findings of the present highlight the regulatory role of linc00507 in tau phosphorylation miR-181c-5p as ceRNA of MAPT/TTBK1 in vitro and in vivo, providing a basis for novel diagnostic and treatment strategies for AD.

12.
J Cell Physiol ; 2020 Aug 23.
Artigo em Inglês | MEDLINE | ID: mdl-32830327

RESUMO

The incidence of acute and chronic wound diseases is rising due to various reasons. With complicated pathogenesis, long course, difficult treatment and high disability, wound diseases have become a major burden for patients, their families, and society. Therefore, the focus of research is to identify new ideas and methods for treatment. Fat grafting has gained increased attention because of its effectiveness in wound treatment, and further analysis has uncovered that the stem cells derived from fat may be the main factor affecting wound healing. We summarize the function of adipose stem cells and analyze their possible mechanisms in tissue repair, helping to provide new ideas for the treatment of wound healing.

13.
Sci Total Environ ; 745: 140839, 2020 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-32726695

RESUMO

Hydrogen (H2) is a universal energy source supplying survival energy for numerous microbial functions. Diffusive fluxes of H2 released by rhizobacterial symbiont nodules in which H2 is an obligate by-product of dinitrogen fixation may act as an additional energy input shaping microbial community structure and function in soils. However, the effects of H2 at the soil-nodule interface on soil contaminant degradation processes are poorly understood. Here, we mimicked the hydrogen conditions present at the soil-nodule interface (10,000 ppmv) to test the impact of elevated H2 concentrations on soil microbial removal of 3, 3', 4, 4'-tetrachlorobiphenyl (PCB77) and examined the associated bacterial communities and their functions by conducting a microcosm experiment using two different soil types at three PCB contamination levels (0.5, 1.0 and 5.0 mg kg-1). After incubation for 84 days the PCB77 removal rates in the elevated H2 treatments in the Paddy soil were significantly promoted (by 4.88 to 6.41%) compared with the control (0.5 ppmv H2) but no significant effect was observed in a Fluvo-aquic soil. This is consistent with changes in the abundance of functional genes for PCB-degraders as shown by quantitative real-time PCR (Q-PCR) and phylogenetic investigation of bacterial communities by reconstruction of unobserved states (PICRUSt). 16S amplicon sequencing was conducted to explore bacterial community structure and correlate the genera to potential PCB degradation. The abundance of a total of four potentially PCB-degrading bacterial genera (Bacillus, Streptomyces, Ramlibacter and Paenibacillus) increased with increasing H2 level. In addition, the abundance of hydrogenase in the elevated H2 treatments was higher than in the control across different contamination levels in both soil types. Thus, elevated H2 stimulated soil PCB degradation with direct effects (aerobic PCB-degrading bacteria directly utilized H2 as an energy source for growth and thus enhanced PCB degradation efficiency) and indirect effects (aerobic PCB-degrading bacteria acted synergistically with other hydrogenotrophs to enhance PCB degradation efficiency by exchange of substances and energy). These results help to further understand the role of elevated hydrogen amendment in the PCB biodegradation process and provide evidence that H2 supports metabolic and energetic flexibility in microorganisms supplying a range of ecosystem services.


Assuntos
Bifenilos Policlorados/análise , Poluentes do Solo/análise , Biodegradação Ambiental , Ecossistema , Hidrogênio , Filogenia , Solo , Microbiologia do Solo
14.
Sci Total Environ ; 742: 140438, 2020 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-32623161

RESUMO

Perfluoroalkyl substance (PFAS) is a class of anionic surfactants with superior stability in the environment. Due to the harmful health effect, PFASs have been listed as the priority controlled pollutants. Our recent study had developed a cationic surfactant induced ternary self-assembled micelle system to effectively degrade PFASs. In this study, using perfluorooctanoic acid (PFOA) as the model pollutant, we further investigated the effects of different variables on the degradation processes. According to the results of laser flash photolysis and dynamic light scattering, the degradation of PFOA was positively correlated with the chain length of the surfactants. While for double-chain surfactant, the steric effect might hinder the reaction. Our results also indicated that in the presence of high concentration of NaCl, the electrostatic attraction between Cl- and the positively charged micelle made the micelle structure loose and thus slightly reduced the degradation efficiency. Similarly, the presence of NOM could also affect the degradation process via regulating the micelle structure. Furthermore, the optimal degradation efficiency for PFOA was obtained at neutral pH by the compromise of hydrated electron yield and self-assembled micelle structure. This composite showed good adaptability under ambient conditions and would have great potential for treatment of industrial PFAS containing wastewater, e.g., in the ternary micelle system, 18.95 mg L-1 PFOA could be completely degraded within 8 h without any pretreatments.

15.
Clin Rheumatol ; 2020 Jun 16.
Artigo em Inglês | MEDLINE | ID: mdl-32557257

RESUMO

OBJECTIVES: To explore the associations of FKBP4 and FKBP5 gene polymorphisms with disease susceptibility, glucocorticoid (GC) efficacy, anxiety, depression, and health-related quality of life (HRQOL) in systemic lupus erythematosus (SLE) patients. METHODS: All subjects were collected from the First and the Second Affiliated Hospital of Anhui Medical University in Hefei, China, during 2011 to 2015. In the case-control study, 541 SLE patients and 543 controls were recruited. In the follow-up study, 466 patients completed the 12-week follow-up and then were divided into GC-sensitive and GC-insensitive groups. Genotyping was determined using Multiplex SNaPshot technique. Data were analyzed using chi-square test and univariate and multivariate logistic regression analyses. RESULTS: rs4713904, rs9368878, and rs7757037 of FKBP5 were associated with depression in SLE patients (rs4713904, PBH = 0.037; rs9368878, PBH = 0.001; rs7757037, PBH = 0.003). Moreover, rs4713904 was associated with GC efficacy in males with SLE (PBH = 0.011). The rs755658 of FKBP5 was associated with improvement in social function (PBH = 0.022) and mental component summary (PBH = 0.028). The rs4713907 of FKBP5 was related to improvement in total score of SF-36, bodily pain, and mental component summary score (all PBH = 0.018). Furthermore, the rs12582595 of FKBP4 was correlated with general health improvement (PBH = 0.033). No associations were seen between FKBP4/FKBP5 gene polymorphisms and SLE susceptibility and anxiety. CONCLUSIONS: FKBP5 gene polymorphisms may be associated with depression and GC efficacy of SLE patients. Meanwhile, the genetic polymorphisms of FKBP4 and FKBP5 genes may be associated with HRQOL improvement in SLE patients.Key Points• FKBP5 gene polymorphisms were associated with depression of SLE patients.• FKBP5 gene polymorphisms were associated with GC efficacy of SLE patients.• FKBP5 gene polymorphisms were associated with HRQOL improvement in SLE patients.• FKBP4 gene polymorphisms were associated with HRQOL improvement in SLE patients.

16.
Ecotoxicol Environ Saf ; 201: 110783, 2020 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-32534333

RESUMO

In this study, 73 samples from soils planted with Panax notoginseng and six P. notoginseng samples were collected in Yunnan Province to investigate the residual levels of six pesticides and their relationships with P. notoginseng and soil. All six pesticides were detected in the soils planted with P. notoginseng located in three regions of Shilin, Kaiyuan, and Yanshan. The detection frequencies of the pesticides in the soils followed the order: quintozene (100%) > iprodione (96%) > procymidone (69%) > chlorothalonil (51%) > pyrimethanil (49%) > pyraclostrobin (29%). The median concentrations of iprodione, pyraclostrobin, pyrimethanil, quintozene, procymidone, and chlorothalonil were 46.40, 6.4, 3.1, 2.86, 2.69, and 0.24 µg/kg, respectively. The mean concentrations of pesticides in the three regions followed the order: Kaiyuan > Shilin > Yanshan, except for iprodione. Furthermore, the concentrations of pesticide residues in soils in each region followed the order: soils never planted with P. notoginseng < soils previously planted with P. notoginseng < soils currently planted with P. notoginseng. The concentration of chlorothalonil in P. notoginseng followed the order: root > stem > leaf, whereas those of the other five pesticides followed the opposite order: root < stem < leaf. There were significant positive correlations between the mean concentrations of pesticides in P. notoginseng and those in the corresponding soils. These results indicate that the rational application of pesticides in P. notoginseng cultivation would be effective for reducing the accumulation of pesticides in P. notoginseng to protect people from the harmful effects of residual pesticides.


Assuntos
Panax notoginseng/fisiologia , Resíduos de Praguicidas/metabolismo , Poluentes do Solo/metabolismo , China , Nitrilos , Nitrobenzenos , Panax notoginseng/química , Resíduos de Praguicidas/análise , Praguicidas , Folhas de Planta/química , Solo/química , Poluentes do Solo/análise
17.
Am J Transl Res ; 12(4): 1267-1274, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32355540

RESUMO

Liver cancer is ranked as the 5th major type of cancer and is responsible for significant number of human deaths across the world. Recent investigations have shown microRNAs (miRs) to be involved in diverse cellular processes. Additionally, they have been shown to exhibit therapeutic implications in treatment of different diseases including cancer. This study investigated the role and therapeutic implications of miR-520b in liver cancer. The results of the present study revealed significant downregulation of miR-520b in liver cancer cell lines. Overexpression of miR-520b suppresses the growth of the SNU-182 and HepG2 cells by triggering apoptosis. This was also accompanied by upsurge of Bax and Caspase-3 and depletion of Bcl-2 in SNU-182 and HepG2 cells. Additionally, miR-520b caused a significant decrease in the migratory and invasive potential of the SNU-182 and HepG2 cells and enhanced their chemosensitivity to Gemcitabine. Bioinformatic analysis and the dual luciferase showed that miR-520b targets IL2 in HepG2 cells. Suppression of IL2 inhibits the growth of the HepG2 cells while as IL2 overexpression could avoid the tumor suppressive effects of miR-520b in HepG2 cells. Taken together, miR-520b may prove to be essential therapeutic target in liver cancer treatment and warrants further research endeavours.

18.
Sci Total Environ ; 731: 138974, 2020 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-32413654

RESUMO

Uptake and metabolism by plants are important biotransformation processes of organic pollutants in ecosystems. However, very limited information is currently available on the metabolism of phthalic acid esters (PAEs) in plants. In this study, alfalfa, highly efficient in phytoremediation of PAE contaminated soil, was chosen as the model to understand the fate of di-n-butyl phthalate (DnBP) in remediation plant. The results of hydroponic experiments indicated that DnBP accumulated mainly in alfalfa roots and adsorption to root epidermis might be the primary uptake mechanism. A large proportion of DnBP was subjected to apparent metabolism. De-esterification could be specified to be the predominant metabolism pathway. Mono-n-butyl phthalate (MnBP) and phthalic acid (PA) were detected as DnBP metabolites in all alfalfa roots and shoots throughout the entire exposure period. Around >90% of MnBP were distributed in cell soluble components and organelles, and MnBP gradually transferred from organelles and cell walls to soluble components as the exposure time extended. Similar to MnBP, PA located mainly in soluble components and organelles as well, while no PA existed in alfalfa cell walls. Exposure to DnBP ultimately resulted in the coexistence of DnBP and MnBP for a long term in interior plants, raising concerns on their combined potential toxicity on plant health or even ecosystem.


Assuntos
Poluentes Ambientais , Ácidos Ftálicos , Dibutilftalato , Ecossistema , Medicago sativa , Solo
19.
Obes Res Clin Pract ; 14(3): 225-233, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32349915

RESUMO

OBJECTIVES: This study sought to examine the variation trends and seasonality of losing weight by using the data from Google Trends tool. METHODS: According to the search term of [lose weight+weight loss], Google Trends data were obtained. Search activity was conducted within the USA, the UK, Canada, Ireland, Australia, and New Zealand from January 01, 2004, to December 31, 2018, utilizing the health category. RESULTS: Dynamic series analysis and the plot of seasonal decomposition of time series show that relative search volume of [lose weight+weight loss] increased from 2004 to 2018 at both national and hemispherical levels. Statistically significant seasonal variations in relative search volume for the term [lose weight+weight loss] were observed using cosinor analyses in the USA (p<0.001), the UK (p<0.001), Canada (p<0.001), Ireland (p<0.001), Australia (p<0.001), and New Zealand (p<0.001), peaking in the spring months and reaching the lowest level in the autumn months. The highest level in spring and the lowest level in autumn were reversed by 6 months in both hemisphere countries, consistent with a seasonal pattern. CONCLUSION: Our results indicate that Internet search queries for losing weight increased within the timeframe of 2004 to 2018, likely reflecting the rising global public interest. In addition, the present research provided preliminary evidence that there is a seasonality of losing weight with a peak in the spring months.

20.
Bull Environ Contam Toxicol ; 105(1): 139-145, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32458034

RESUMO

In this study, the responses of wheat seedlings to graphene oxide (GO) were investigated at a wide concentration range of 0-1000 mg L-1, including oxidative stress, real-time membrane potential as well as proton and calcium ion fluxes. The results show that GO induced a hormesis effect on root growth (low concentration (100 mg L-1) promotion and high concentration (1000 mg L-1) inhibition. Oxidative stress was responsible for the growth inhibition at GO concentration of 1000 mg L-1, as suggested from great stimulation in the activities of antioxidant enzymes and MDA content in roots or leaves. Superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) activities were highly correlated with MDA levels (r2 = 0.963, 0.984, and 0.960, respectively). GO exposure caused significant concentration-dependent membrane depolarization in roots, and significantly inhibited H+ efflux and extracellular Ca2+ influx in root cap.


Assuntos
Grafite/toxicidade , Triticum/efeitos dos fármacos , Antioxidantes/farmacologia , Catalase/metabolismo , Hormese/efeitos dos fármacos , Malondialdeído , Oxirredução , Estresse Oxidativo/efeitos dos fármacos , Estresse Oxidativo/fisiologia , Folhas de Planta/metabolismo , Raízes de Plantas/metabolismo , Plântula/efeitos dos fármacos , Superóxido Dismutase/metabolismo
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