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1.
Food Chem ; 305: 125457, 2020 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-31505414

RESUMO

Taking into account a growing market and small number of articles related to honeydew honey, a metabolomic approach associated with multivariate analysis and modelling was proposed to discriminate five varieties of honey. Advanced analytical techniques were used for determination of 20 elements, 14 carbohydrates and stable carbon isotope ratio. No chemical marker has been found within sugar compounds, but several elements (Ba, Ca, Mg, Sr, Mn, Al, Co, Ni, Se) were marked as characteristic of honey type and allow classification of three botanical origins (Abies alba, Quercus frainetto, Quercus ilex). Sugars turanose, trehalose, arabinose and raffinose, elements Ba, Sr, P, Cd and Se, and δ13C values of honey, have different concentrations in honeys of the same botanical origin but harvested in different season. In addition to a confirmation of authenticity in terms of production, the values of δ13C of protein could be a good indicator of botanical origin.

2.
Biomolecules ; 9(12)2019 Nov 26.
Artigo em Inglês | MEDLINE | ID: mdl-31779186

RESUMO

Information on compositional, nutritional and functional properties of bee-pollen, as a health-promoting food, is essential for defining its quality. Concerning the nutritional importance of phenolic compounds, the aim of this study was to determine the phenolic profile and antioxidant activity of twenty-four bee-pollen samples collected from different regions of Serbia. High-performance thin-layer chromatographic (HPTLC) fingerprinting was used for profiling of bee-pollen samples according to the botanical type. HPTLC hyphenated with image analysis and a pattern recognition technique confirmed the grouping of samples caused by the specific phenolic composition of pollens of different botanical origin. Flavonoid glycosides in bee-pollen samples were identified by applying ultra-high-performance liquid chromatography (UHPLC) coupled with linear ion trap-Orbitrap mass spectrometry (LTQ Orbitrap MS). Eight out of twenty-seven flavonol glycosides were identified in bee-pollen samples for the first time. All analyzed bee-pollen samples showed a high number of phenolic compounds which may have therapeutic potential.

3.
Molecules ; 24(21)2019 Nov 04.
Artigo em Inglês | MEDLINE | ID: mdl-31689909

RESUMO

In recent years, the progress of science and medicine greatly has influenced human life span and health. However, lifestyle habits, like physical activity, smoking cessation, moderate alcohol consumption, diet, and maintaining a normal body weight represent measures that greatly reduce the risk of various diseases. The type of diet is very important for disease development. Numerous epidemiological clinical data confirm that longevity is linked to predominantly plant-based diets and it is related to a long life; whereas the western diet, rich in red meat and fats, increases the risk of oxidative stress and thus the risk of developing various diseases and pre-aging. This review is focused on the bioavailability of polyphenols and the use of polyphenols for the prevention of prostate diseases. Special focus in this paper is placed on the isoflavonoids and flavan-3-ols, subgroups of polyphenols, and their protective effects against the development of prostate diseases.

4.
Molecules ; 24(11)2019 Jun 04.
Artigo em Inglês | MEDLINE | ID: mdl-31167390

RESUMO

The production of secondary metabolites in tissue culture has been considered as an alternative to the cultivation and harvesting of crops intended for this purpose. The present study was aimed at the growth of callus and production of polyphenolic compound of callus derived from a Maltese indigenous grapevine variety, Gellewza. Callus was inoculated onto plant growth regulators-enriched Murashige Skoog media (MSm) to determine whether polyphenols are produced in vitro as well as to determine the best combination of plant growth regulators needed for the production of these metabolites. From results obtained, it was observed that the best callus production was obtained by auxin-enriched MSm. In fact, indole acetic acid and indole acetic acid /6-benzyl aminopurine enhanced biomass accumulation (3.04 g and 3.39 g) as opposed to the others (<1.97 g). On the other hand, parameters showing the presence of flavonoids (tonality, 3.80), particularly anthocyanins (24.09 mg/kg) and total polyphenols (1.42 mg/g), were optimum in the presence of cytokinins, particularly 6-benzyl aminopurine. Analysis for single polyphenols revealed a high amount a particular stilbene: polydatin (glucoside of resveratrol). Resveratrol and other typical polyphenols, found in mature berries, were also found in significant quantities, while the other polyphenolic compounds were found in minimal quantities. This is the first study to describe the production and composition of polyphenols in Gellewza callus cultures. From the results obtained, it can be seen that this grape tissue is an excellent alternative for the production of polyphenols from the stilbene group, which can be upscaled and exploited commercially.


Assuntos
Estilbenos/metabolismo , Vitis/metabolismo , Biomassa , Fenômenos Químicos , Cromatografia Líquida , Espectrometria de Massas , Estrutura Molecular , Reguladores de Crescimento de Planta/metabolismo , Polifenóis/química , Polifenóis/metabolismo , Estilbenos/química , Vitis/química , Vitis/crescimento & desenvolvimento
5.
Food Chem ; 284: 28-44, 2019 Jun 30.
Artigo em Inglês | MEDLINE | ID: mdl-30744859

RESUMO

The aim of this study was to evaluate the effect of enriching a complex food matrix (FM) with grape extracts on polyphenol content, composition, bioaccessibility and antioxidant activity during digestion. The grape extracts and FM were separately tested under the same conditions as controls. The FM by itself contains a significant amount of phenolic acids and flavonols, influencing the final recovery of polyphenols from grape extracts. The FM significantly increased the total recovery of polyphenols after digestion of grape seed extracts compared to those digested without the FM; however, a low recovery of proantocyanidins and total flavonoids was observed. Digestive fluids and FM compounds significantly increased the total polyphenol content of grape digests and significantly contributed to their ABTS+ scavenging activity and ferrous-ion-chelating capacity. The present study suggested that enrichment of meat- and cereal-based products with grape polyphenol extracts could be a good strategy to formulate a healthier diet.


Assuntos
Digestão , Grão Comestível/metabolismo , Alimentos Fortificados/análise , Carne/análise , Extratos Vegetais/análise , Vitis , Antioxidantes/análise , Disponibilidade Biológica , Grão Comestível/química , Flavonoides/análise , Flavonóis , Humanos , Polifenóis/análise , Proantocianidinas/análise , Sementes/química
6.
Toxins (Basel) ; 11(2)2019 01 24.
Artigo em Inglês | MEDLINE | ID: mdl-30678361

RESUMO

Due to its divergent chemical composition and good nutritional properties, pollen is not only important as a potential food supplement but also as a good substrate for the development of different microorganisms. Among such microorganisms, toxigenic fungi are extremely dangerous as they can synthesize mycotoxins as a part of their metabolic pathways. Furthermore, favorable conditions that enable the synthesis of mycotoxins (adequate temperature, relative humidity, pH, and aw values) are found frequently during pollen collection and/or production process. Internationally, several different mycotoxins have been identified in pollen samples, with a noted predominance of aflatoxins, ochratoxins, fumonisins, zearalenone, deoxynivalenol, and T-2 toxin. Mycotoxins are, generally speaking, extremely harmful for humans and other mammals. Current EU legislation contains guidelines on the permissible content of this group of compounds, but without information pertaining to the content of mycotoxins in pollen. Currently only aflatoxins have been researched and discussed in the literature in regard to proposed limits. Therefore, the aim of this review is to give information about the presence of different mycotoxins in pollen samples collected all around the world, to propose possible aflatoxin contamination pathways, and to emphasize the importance of a regular mycotoxicological analysis of pollen. Furthermore, a suggestion is made regarding the legal regulation of pollen as a food supplement and the proposed tolerable limits for other mycotoxins.

7.
Chem Biodivers ; 16(3): e1800565, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30609204

RESUMO

This study was aimed at investigating the phytochemical composition and antioxidant capacity of rhizomes, above-ground vegetative parts and flowers of three Iris species: Iris humilis Georgi, Iris pumila L. and Iris variegata L. UHPLC-Orbitrap MS analysis was used for determination of phytochemical profile. Total pigments, phenolics, flavonoids, soluble sugars and starch content as well as ABTS antioxidant capacity were also determined. In total, 52 phenolics compounds were identified with 9 compounds (derivatives of iriflophenone, apigenin C-glycosides, luteolin O-glycoside, isoflavones derivatives of iristectorigenin, dichotomitin, nigracin and irilone) never reported before in Iris spp. Differences in phenolic composition profile, pigments, soluble sugar, starch, total phenolics and flavonoids content and total antioxidant capacity were found among Iris species and different part of plants. Significant correlation between total phenolic content and antioxidant capacity was determined. The obtained results are comparable with those obtained for medical plants. These findings could be useful for fingerprinting characterization of Iris species and estimation of possible use in pharmaceutical industries.


Assuntos
Antioxidantes/farmacologia , Benzotiazóis/antagonistas & inibidores , Flores/química , Iris (Planta)/química , Compostos Fitoquímicos/farmacologia , Componentes Aéreos da Planta/química , Ácidos Sulfônicos/antagonistas & inibidores , Antioxidantes/análise , Iris (Planta)/classificação , Estrutura Molecular , Compostos Fitoquímicos/análise , Especificidade da Espécie
8.
Food Chem ; 274: 629-641, 2019 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-30372988

RESUMO

Concerning the particular nutritive value of honeydew honey compared to blossom honey, and small number of studies defining botanical origin of honeydew honey, comprehensive analysis of phenolic profile of 64 honeydew honey samples of specific botanical origin was performed. Two advanced techniques of liquid chromatography hyphenated with mass spectrometry were used for identification of a total of 52 compounds and quantification of 25 of them. Pattern recognition analysis applied on data on phenolic compounds content confirmed that quercetin, naringenin, caffeoylquinic acid, hydroxyphenylacetic acid, apigenin and genistein, could be considered as potential markers of botanical origin of honeydew honey. Spectroscopic and electrochemical approaches were applied for the evaluation of the antioxidant capacity. Quercus sps. samples, Quercus frainetto and Quercus ilex, showed high biological activity and specific chemical composition. Additionally, cyclic voltammetry profiles were used for characterization and natural clustering of honeydew honey for the first time.


Assuntos
Antioxidantes/química , Mel/análise , Fenóis/análise , Quercus/química , Cromatografia Líquida de Alta Pressão , Análise por Conglomerados , Técnicas Eletroquímicas , Espectrometria de Massas , Valor Nutritivo , Análise de Componente Principal , Quercus/metabolismo
9.
Food Chem ; 271: 328-337, 2019 Jan 15.
Artigo em Inglês | MEDLINE | ID: mdl-30236684

RESUMO

The main goal of this research was to determine the relationship among chemical structure, bioactivity and temperature of chamomile during subcritical water extraction in isobaric conditions (45 bar) at seven different temperatures (65-210 °C). The influence of temperature on phenolic profiles was defined by UHPLC-HESI-MS/MS. The overall results indicate that the presence of conjugated double bonds, side chains, glucose moiety or ether moiety in molecules influence the efficiency of polyphenols' extraction in subcritical water. In terms of antioxidant activity, the extracts were the most active towards ABTS radicals (IC50 = 7.3-16.8 µg/mL), whereby temperature of 150 °C was optimal. On the other hand, the extracts obtained at 115 °C showed highest cytotoxicity. Inhibition of α-amylase and α-glucosidase was the highest at 65 and 85 °C, i.e. 0.51 and 4.13 mmolAE/g, respectively. Activity against tyrosinase was the highest at 210 °C (17.92 mgKAE/g). The data showed that different non-phenolic compounds may also participate in bio-activities of the extracts.


Assuntos
Matricaria/química , Polifenóis/análise , Temperatura Ambiente , Água/química , Antioxidantes , Extratos Vegetais/química , Espectrometria de Massas em Tandem
10.
Food Chem ; 266: 389-396, 2018 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-30381203

RESUMO

The study was designed to determine the relationship between chemical structure, bioactivity and pressure during the subcritical water extraction (SCW) of chamomile. Extraction was carried out at isothermal conditions (100 °C) at five different pressures (10, 30, 45, 60 and 90 bar). Twenty three polyphenolic compounds were identified in the extracts, whereby apigenin was found to be the dominant compound (61.53-1344.99 mg/kg). Results suggest that the lowest applied pressure has negligible effect on phenolic recovery from chamomile, but also the use of pressures above than 45 bar was proven as needlessly. By using in vitro assays, influence of pressure on antioxidant, cytotoxic and enzyme-inhibitory activities of the extracts was evaluated. Extracts obtained at 30, 45 and 60 bar exhibited stronger bioactivities than at 10 and 90 bar. It was concluded that pressure exert a significant influence on chemical composition of extracts, and thus on biological activity of chamomile extracts.

11.
Nat Prod Res ; : 1-5, 2018 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-29600716

RESUMO

The elemental profile of the leaf samples of three Cabernet Franc clone candidates recently developed in Serbia (Nos. 02, 010 and 012) obtained in the last phase of clonal selection was examined within this study by ICP-OES. Optimal content of Al, Fe, Cu and Zn, the metals well known for their links with a number of neurodegenerative disorders including Alzheimer's disease, has actually highlighted the potential of the leaf sample No. 10 to afford a novel food supplement of natural origin possessing well balanced metal ingredients. Furthermore, lower content of some other elements (Cr, Mn, Ni and Pb) thoroughly supports such a claiming. Finally, the most favourable K/Na ratio observed for the aforementioned sample points out its likely cardioprotectivity. However, two other Cabernet Franc clone candidates might also be recommended for breeding in the same or similar viticultural conditions, since all obtained values were below toxic ones for human consumption.

12.
Prostate ; 77(10): 1082-1092, 2017 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-28594074

RESUMO

BACKGROUND: There is still no consensus regarding intraprostatic androgen levels and the accumulation of androgens in the hyperplastic prostatic tissue. The current opinion is that intraprostatic dihydrotestosterone (DHT) concentrations are maintained but not elevated in benign prostatic hyperplasia (BPH), while there is no similar data concerning intraprostatic testosterone (T). METHODS: Tissue T (tT) and tissue DHT (tDHT) concentration were determined in 93 patients scheduled for initial prostate biopsy. The criteria for biopsy were abnormal DRE and/or PSA > 4 ng/mL. Total prostate volume (TPV) was determined by transrectal ultrasound (TRUS). During TRUS- guided prostate biopsy, 10-12 samples were collected from the peripheral zone (PZ) and two additional samples were collected from the transition zone (TZ). The samples from the TZ were immediately frozen in liquid nitrogen at -70°C, and transported for tissue androgen determination, using liquid chromatography mass spectrometry (LC-MS). RESULTS: Pathological analysis revealed that prostate cancer (PCa) was present in 45 and absent in 48 patients. In the whole group, there were 42 men with small prostate (TPV < 30 mL) and 51 with enlarged prostate (TPV ≥ 31 mL). The overall average tT level was 0.79 ± 0.66 ng/g, while the average tDHT level was 10.27 ± 7.15 ng/g. There were no differences in tT and tDHT level in prostates with and without PCa. However, tT and tDHT levels were significantly higher in larger, than in smaller prostates (tT: 1.05 ± 0.75 and 0.46 ± 0.29 ng/g, and tDHT: 15.0 ± 6.09 and 4.51 ± 2.75 ng/g, respectively). There were strong correlations between tT and TPV (r = 0.71), and tDHT and TPV (r = 0.74). CONCLUSIONS: The present study confirmed that both T and DHT accumulated in the stroma of enlarged prostates; the degree of accumulation correlated with prostate volume.


Assuntos
Androgênios/metabolismo , Di-Hidrotestosterona/metabolismo , Próstata , Hiperplasia Prostática , Testosterona/metabolismo , Idoso , Biópsia/métodos , Cromatografia Líquida/métodos , Humanos , Masculino , Pessoa de Meia-Idade , Tamanho do Órgão , Próstata/metabolismo , Próstata/patologia , Hiperplasia Prostática/metabolismo , Hiperplasia Prostática/patologia , Estatística como Assunto
13.
14.
J AOAC Int ; 100(4): 840-851, 2017 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-28527181

RESUMO

Honey is a complex mixture of various substances, and its composition depends on both botanical and geographical origin, as well as anthropogenic factors. The accurate identification of honey origin guarantees the satisfaction of consumers' needs and has an impact on the honey market value. Physicochemical parameters, some of which are used in routine analysis of honey quality, could be useful for the assessment of its origin. In this review, special attention is paid to those studies that assessed the sugar and mineral composition of honey, whether they were investigated in terms of botanical or geographical origin, or for the characterization of honey type. The oligosaccharides present in honey and the electrical conductivity of honey correlate strongly with its botanical origin. Mineral content could be indicative for distinguishing honeys according to their botanical and geographical origins because it depends on both the soil composition and the floral type of melliferous plants. This review provides insight into the results obtained by various studies from approximately the last 10 years concerning the sugar profile and the mineral and trace element content of different types of honey. An attempt was made to statistically analyze the results regarding mineral and trace element content in order to identify indicators that could distinguish honey by origin.


Assuntos
Carboidratos/análise , Mel/análise , Minerais/análise
15.
J AOAC Int ; 100(4): 852-861, 2017 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-28527184

RESUMO

In recent years, the botanical and geographical origin of food has become an important topic in the context of food quality and safety, as well as consumer protection, in accordance with international standards. Finding chemical markers, especially phytochemicals, characteristic for some kind of food is the subject of interest of a significant number of researchers in the world. This paper is focused on the use of polyphenols as potential markers for the determination of botanical origin of honey. It includes a review of the polyphenols present in various honey samples and the methods for their separation and identification. Special emphasis in this paper is placed on the identification of honey polyphenols using advanced LC-MS techniques in order to find specific markers of botanical origin of honey. In this regard, this study gives an overview of the literature that describes the use of LC-MS techniques for the isolation and determination of honey polyphenols. This review focuses on the research performed in the past two decades.


Assuntos
Mel/análise , Polifenóis/análise , Cromatografia Líquida , Qualidade dos Alimentos , Espectrometria de Massas
16.
Chemosphere ; 171: 284-293, 2017 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28027472

RESUMO

A different single extraction procedures (CH3COOH, Na2EDTA, CaCl2, NH4NO3, deionized water), and pseudo-total digestion (aqua regia) were applied to determine major (Al, Fe, K, Mn, Na, P, S, and Si) and trace (Cd, Co, Cr, Cu, Mo, Ni, Pb, V, and Zn) element bioavailability in a topsoil from the experimental vineyard ("Radmilovac", Belgrade, Serbia). For the first time, the extraction with deionized water during 16 h was tested as an alternative method for isolating bioavailable major and trace elements from the soil. Concentrations of the elements were determined by inductively coupled plasma - optical emission spectroscopy (ICP-OES). The extraction of Cu and S from the soil by deionized water during 16 h extracting, NH4NO3, and CaCl2 indicated that these elements could originate from the anthropogenic sources, such as fungicide. In addition according to the soil - plant experiment, performed as a preliminary experiment for future studies in vineyards, deionized water was recommended for isolation of bioavailable elements from grape seed and grape pulp; CH3COOH, Na2EDTA, CaCl2 and NH4NO3 for grape skin, while for assessment of leaf bioavailable elements from soil fraction, aqua regia was recommended. In addition, identification of similarities between the plant parts and the plant species were performed. Applying environmental risk assessment formulas, the most polluted vineyard parcel in the vineyard region "Radmilovac" was determined. The leaves of some grapevine species showed the high ability for accumulation some of the potentially toxic trace elements from the soil.


Assuntos
Poluição Ambiental/análise , Folhas de Planta/química , Solo/química , Oligoelementos/análise , Vitis/química , Disponibilidade Biológica , Fazendas , Sérvia
17.
Food Chem ; 217: 705-715, 2017 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-27664689

RESUMO

Spice peppers (Capsicum annuum L.) var. Lemeska and Lakosnicka paprika were investigated to evaluate their polyphenolic and carbohydrate profiles and antioxidant activity. A total of forty-nine polyphenolics were identified using ultrahigh-performance liquid chromatography (UHPLC) coupled to LTQ OrbiTrap mass analyzer. Twenty-five of them were quantified using available standards, while the other compounds were confirmed by exact mass search of their deprotonated molecule [M-H](-) and its MS(4) fragmentation. Thirteen carbohydrates were quantified using high-performance anion exchange chromatography (HPAEC) with pulsed amperometric detection (PAD). Radical scavenging activity (RSA) ranged from 17.32 to 48.34mmol TE (Trolox equivalent)/kg DW (dry weight) and total phenolics content (TPC) was ranged between 7.03 and 14.92g GAE (gallic acid equivalents)/kg DW. To our best knowledge, five polyphenolic compounds were for the first time tentatively identified in paprika: 5-O-p-coumaroylquinic acid, luteolin 7-O-(2″-O-pentosyl-4″-O-hexosyl)hexoside, quercetin 3-O-(2″-O-hexosyl)rhamnoside, isorhamnetin 3-O-[6″-O-(5-hydroxyferuloyl)hexoside]-7-O-rhamnoside, and luteolin 7-O-[2″-O-(5'″-O-sinapoyl)pentosyl-6″-O-malonyl]hexoside.


Assuntos
Capsicum/química , Carboidratos/análise , Polifenóis/análise , Especiarias/análise , Capsicum/classificação , Cromatografia Líquida de Alta Pressão/métodos , Geografia , Luteolina/análise , Extratos Vegetais/análise , Quercetina/análogos & derivados , Sérvia
18.
Environ Sci Pollut Res Int ; 23(21): 22084-22098, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27541154

RESUMO

The content and bioaccumulation of trace (Ag, Se, As) and major elements (Ca, Mg, Na and K) in wild edible mushroom Macrolepiota procera and its corresponding soil substrates, collected from five sites in the Rasina region in central Serbia, were investigated. The content of Ag, As and Se was determined by inductively coupled plasma mass spectrometer (ICP-MS) while the amount of Ca, Mg, Na and K was determined by inductively coupled plasma optical emission spectrometer (ICP-OES). The concentrations of major elements in the mushrooms were at typical levels. As far as trace elements are concerned, M. procera bioaccumulates silver although all samples were collected from unpolluted sites. It was found that the content of Ag depended on the geographical origin and the density of fruiting body on the certain site. Principal component analysis distinguished the mushroom samples from different geographical areas and revealed the influence of soil composition on metal content in fruiting bodies. Also, a linear regression correlation test was performed to investigate correlations between Ag, Cd, Se, Pb and As in caps and stipes at different geographic sites separately. In addition, our results indicated that M. procera could serve as a good dietary source of Mg, K and Se. The content of Ag and As was low, so it could not pose a health risk for consumers.


Assuntos
Agaricales/metabolismo , Arsênico/metabolismo , Contaminação de Alimentos/análise , Metais Pesados/metabolismo , Poluentes do Solo/metabolismo , Arsênico/análise , Monitoramento Ambiental , Humanos , Metais Pesados/análise , Medição de Risco , Selênio/análise , Selênio/metabolismo , Sérvia , Prata/análise , Prata/metabolismo , Poluentes do Solo/análise
19.
Nutr Cancer ; 68(6): 988-1000, 2016 Aug-Sep.
Artigo em Inglês | MEDLINE | ID: mdl-27348025

RESUMO

To date little has been done on identification of major phenolic compounds responsible for anticancer and antioxidant properties of pea (Pisum sativum L.) seed coat extracts. In the present study, phenolic profile of the seed coat extracts from 10 differently colored European varieties has been determined using ultrahigh-performance liquid chromatography-linear trap quadrupole orbitrap mass spectrometer technique. Extracts of dark colored varieties with high total phenolic content (up to 46.56 mg GAE/g) exhibited strong antioxidant activities (measured by 2,2-diphenyl-1-picrylhydrazyl or DPPH assay, and ferric ion reducing and ferrous ion chelating capacity assays) which could be attributed to presence of gallic acid, epigallocatechin, naringenin, and apigenin. The aqueous extracts of dark colored varieties exert concentration-dependent cytotoxic effects on all tested malignant cell lines (human colon adenocarcinoma LS174, human breast carcinoma MDA-MB-453, human lung carcinoma A594, and myelogenous leukemia K562). Correlation analysis revealed that intensities of cytotoxic activity of the extracts strongly correlated with contents of epigallocatechin and luteolin. Cell cycle analysis on LS174 cells in the presence of caspase-3 inhibitor points out that extracts may activate other cell death modalities besides caspase-3-dependent apoptosis. The study provides evidence that seed coat extracts of dark colored pea varieties might be used as potential cancer-chemopreventive and complementary agents in cancer therapy.


Assuntos
Anticarcinógenos/análise , Antioxidantes/análise , Flavonoides/análise , Ervilhas/química , Fenóis/análise , Epiderme Vegetal/química , Sementes/química , Anticarcinógenos/química , Anticarcinógenos/metabolismo , Antioxidantes/metabolismo , Apigenina/análise , Apigenina/metabolismo , Catequina/análogos & derivados , Catequina/análise , Catequina/metabolismo , Linhagem Celular Tumoral , Sobrevivência Celular , Croácia , Produtos Agrícolas/química , Produtos Agrícolas/metabolismo , Suplementos Nutricionais/análise , Flavanonas/análise , Flavanonas/metabolismo , Flavonoides/metabolismo , Ácido Gálico/análise , Ácido Gálico/metabolismo , Humanos , Quelantes de Ferro/análise , Quelantes de Ferro/metabolismo , Luteolina/análise , Luteolina/metabolismo , Ervilhas/metabolismo , Fenóis/metabolismo , Pigmentos Biológicos/biossíntese , Epiderme Vegetal/metabolismo , Extratos Vegetais/química , Extratos Vegetais/metabolismo , Análise de Componente Principal , Sementes/metabolismo
20.
Food Chem ; 211: 243-52, 2016 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-27283628

RESUMO

Phenolic profile of 13 grapevine varieties was determined, with respect to three different parts of berries: skin, seed and pulp. Identification and quantification of the phenols was done using ultra-high performance liquid chromatography (UHPLC) coupled with a diode array detector and a triple-quadrupole mass spectrometer. The most abundant phenols in grape seeds were found to be flavan-3-ols, most of which are gallocatechin gallate and catechin. The skins were characterized mostly by flavonols, i.e. quercetin and myricetin. Characterization of anthocyanins in the berry skin by UHPLC coupled with Linear Trap Quadrupole and OrbiTrap mass analyzer revealed a total of twenty derivatives of malvidin, delphinidin, petunidin, cyanidin, and peonidin. To the extent of our knowledge this is the first work that shows the presence of malvidin 3,5-O-dihexoside in the berry skin of 'Merlot', 'Cabernet Franc', 'Shiraz' 'Sangiovese', 'Pinot Noir' and 'Prokupac', untypical for Vitis vinifera Linneo species.


Assuntos
Análise de Alimentos/métodos , Frutas/química , Fenóis/análise , Vitis/química , Antocianinas/análise , Catequina/análogos & derivados , Catequina/análise , Cromatografia Líquida de Alta Pressão , Flavonoides/análise , Flavonóis/análise , Depuradores de Radicais Livres , Espectrometria de Massas , Extratos Vegetais/química , Polifenóis/análise , Sementes/química , Sérvia
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