Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 11 de 11
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Vet Parasitol ; 277: 109015, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31874403

RESUMO

Canine leishmaniosis (CanL)-associated chronic kidney disease is a leading cause of morbidity and mortality in Mediterranean countries. Novel renal biomarkers, such as serum symmetric dimethylarginine (sSDMA), may be useful surrogates for the detection of renal functional impairment. The objectives of this study were to investigate sSDMA concentrations in dogs with CanL, with and without azotemia, and to establish any potential association with the prevalence and severity of proteinuria, with the prevalence of decreased urine specific gravity and with the LeishVet clinical stages of CanL. Serum samples from 68 dogs with CanL (50 nonazotemic and 18 azotemic) and 17 healthy dogs were retrospectively examined. Increased sSDMA was documented in 26 % of dogs with CanL without azotemia and in 83.3 % of dogs with azotemia. Serum SDMA was significantly higher in azotemic compared to nonazotemic dogs and was associated with the presence and severity of proteinuria, the decreased urine specific gravity and the advanced clinical stages of CanL. The results of the present study indicate that sSDMA may be a useful adjunct to serum creatinine and urine protein/creatinine ratio for the detection of CanL-associated nephropathy, but it is of limited value for distinguishing among the LeishVet clinical stages of CanL.


Assuntos
Arginina/análogos & derivados , Doenças do Cão/diagnóstico , Leishmaniose Visceral/complicações , Leishmaniose Visceral/diagnóstico , Insuficiência Renal Crônica/etiologia , Animais , Arginina/sangue , Azotemia/veterinária , Biomarcadores/sangue , Doenças do Cão/sangue , Cães , Leishmania infantum/fisiologia , Insuficiência Renal Crônica/sangue , Estudos Retrospectivos
2.
Vet J ; 217: 109-111, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27810200

RESUMO

Canine monocytic ehrlichiosis (CME, Ehrlichia canis) has occasionally been associated with myocardial injury. The aim of the present study was to serially measure and evaluate cardiac troponin I (cTnI) concentrations in dogs with experimentally induced acute and subclinical CME and to evaluate potential associations between cTnI concentration and an array of echocardiographic and electrocardiographic parameters. Serum cTnI concentration and simultaneous echocardiographic and electrocardiographic recordings were evaluated in 12 healthy Beagle dogs prior to experimental infection and on days 20 and 90 post-inoculation with E. canis. Almost all serum cTnI concentrations were below the limit of detection and selected electrocardiographic and echocardiographic parameters remained unchanged throughout the study.


Assuntos
Doenças do Cão/diagnóstico por imagem , Ehrlichia canis/fisiologia , Ehrlichiose/veterinária , Troponina I/sangue , Doença Aguda , Animais , Infecções Assintomáticas , Doenças do Cão/microbiologia , Cães , Ecocardiografia/veterinária , Ehrlichiose/diagnóstico por imagem , Ehrlichiose/microbiologia , Eletrocardiografia/veterinária , Feminino , Masculino , Monócitos/microbiologia
3.
Vet Clin Pathol ; 45(2): 281-90, 2016 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-27142117

RESUMO

BACKGROUND: Canine monocytic ehrlichiosis (CME) is one of the most important tick-borne diseases worldwide. Cytopenias have been observed in both acute (nonmyelosuppressive) and chronic (myelosuppressive) CME; however, leukocyte abnormalities and indices have been incompletely described in dogs with acute CME. OBJECTIVES: The aims of this study were to analyze temporal changes in differential leukocyte counts, leukocyte morphology, myeloperoxidase index (MPXI), and lobularity index (LI) in dogs with experimental and naturally occurring acute CME. METHODS: Differential leukocyte counts and morphology were evaluated in archived blood smears from 13 Beagle dogs experimentally infected with Ehrlichia canis and evaluated weekly for 42 days postinfection (DPI); 20 dogs with naturally occurring acute CME also were evaluated. MPXI and LI were obtained from ADVIA reports. Wilcoxon tests were used to assess changes over time; leukogram results in natural cases were assessed in comparison with reference intervals. RESULTS: In experimental dogs, significant decreases in neutrophil, monocyte, lymphocyte, and eosinophil counts, and a mild left shift occurred within 14 DPI. The MPXI decreased significantly between 14 and 21 DPI and remained low, while LI increased from 14 to 35 DPI. Lymphocyte counts rebounded at 21 DPI, normalizing total WBC counts. Neutrophil toxicity was seen rarely, but reactive lymphocytes were observed frequently. Dogs with natural infection had variable patterns of leukocyte changes. CONCLUSIONS: Acute CME is associated with several discrete quantitative and qualitative leukogram changes indicative of concurrent inflammation, antigenic stimulation, and stress. Changes in MPXI and LI warrant further investigation in dogs with CME and other diseases.


Assuntos
Doenças do Cão/sangue , Ehrlichia canis , Ehrlichiose/veterinária , Leucócitos/patologia , Animais , Doenças do Cão/microbiologia , Doenças do Cão/patologia , Cães , Ehrlichiose/sangue , Feminino , Contagem de Leucócitos/veterinária , Leucócitos/enzimologia , Masculino , Peroxidase/sangue
4.
Vet Microbiol ; 184: 59-63, 2016 Feb 29.
Artigo em Inglês | MEDLINE | ID: mdl-26854345

RESUMO

There is currently lack of information on the changes of acute phase proteins (APP) and antioxidant markers and their clinical relevance as treatment response indicators in canine monocytic ehrlichiosis (CME). The objective of this study was to investigate the patterns of C-reactive protein (CRP), haptoglobin (Hp), ferritin and paraoxonase-1 (PON-1) during treatment of dogs with acute CME with rifampicin. Blood serum samples from ten Beagle dogs with experimental acute CME were retrospectively examined. Five dogs (Group A) were treated with rifampicin (10mg/Kg/24h), per os, for 3 weeks and 5 dogs (Group B) received no treatment (infected controls). Two Beagle dogs served as uninfected controls. Blood serum samples were serially examined prior to Ehrlichia canis inoculation and on post-inoculation days 14, 21, 28, 35 and 42. Significant changes of CRP, Hp, ferritin and PON-1 values were found in the majority of infected dogs. However, their concentrations did not differ between the two groups during the treatment observation period. The results of this study indicate that although several APP and PON-1 tend to significantly change in the majority of dogs with acute CME, they were of limited clinical relevance as treatment response indicators in this experimental setting.


Assuntos
Biomarcadores/sangue , Doenças do Cão/tratamento farmacológico , Doenças do Cão/imunologia , Ehrlichiose/veterinária , Rifampina/uso terapêutico , Proteínas da Fase Aguda/imunologia , Animais , Arildialquilfosfatase/sangue , Proteína C-Reativa/imunologia , Doenças do Cão/sangue , Cães , Ehrlichiose/sangue , Ehrlichiose/tratamento farmacológico , Ehrlichiose/imunologia , Feminino , Ferritinas/sangue , Haptoglobinas/imunologia , Masculino
5.
PLoS One ; 11(2): e0148428, 2016.
Artigo em Inglês | MEDLINE | ID: mdl-26849209

RESUMO

Toll-like receptors (TLRs) are central sensors for the inflammatory response in ischemia-reperfusion injury. We therefore investigated whether TLR4 inhibition could be used to treat stroke in a standard model of focal cerebral ischemia. Anti-TLR4/MD2-antibody (mAb clone MTS510) blocked TLR4-induced cell activation in vitro, as reported previously. Here, different routes of MTS510 application in vivo were used to study the effects on stroke outcome up to 2d after occlusion of the middle cerebral artery (MCAO) for 45 min in adult male C57Bl/6 wild-type mice. Improved neurological performance, reduced infarct volumes, and reduced brain swelling showed that intravascular application of MTS510 had a protective effect in the model of 45 min MCAO. Evaluation of potential long-term adverse effects of anti-TLR4-mAb-treament revealed no significant deleterious effect on infarct volumes nor neurological deficit after 14d of reperfusion in a mild model of stroke (15 min MCAO). Interestingly, inhibition of TLR4 resulted in an altered adaptive immune response at 48 hours after reperfusion. We conclude that blocking TLR4 by the use of specific mAb is a promising strategy for stroke therapy. However, long-term studies with increased functional sensitivity, larger sampling sizes and use of other species are required before a clinical use could be envisaged.


Assuntos
Anticorpos Monoclonais/farmacologia , Acidente Vascular Cerebral/tratamento farmacológico , Receptor 4 Toll-Like/imunologia , Imunidade Adaptativa/efeitos dos fármacos , Animais , Anticorpos Monoclonais/administração & dosagem , Edema Encefálico/tratamento farmacológico , Isquemia Encefálica/tratamento farmacológico , Isquemia Encefálica/patologia , Modelos Animais de Doenças , Infarto da Artéria Cerebral Média/tratamento farmacológico , Infarto da Artéria Cerebral Média/fisiopatologia , Injeções Intra-Arteriais , Injeções Intraperitoneais , Injeções Intravenosas , Masculino , Camundongos Endogâmicos C57BL , Transdução de Sinais/efeitos dos fármacos , Acidente Vascular Cerebral/patologia , Receptor 4 Toll-Like/antagonistas & inibidores , Receptor 4 Toll-Like/metabolismo
6.
J Am Anim Hosp Assoc ; 51(5): 338-41, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26355586

RESUMO

A 9 yr old cat was presented with clinical signs and laboratory abnormalities attributed to arterial hypertension (mean systolic arterial pressure, 290 mm Hg). Plasma aldosterone concentration was increased at the time of admission (651 pmol/L), but serum creatinine and potassium concentrations were within the reference range. A second increased aldosterone (879 pmol/L) and normal plasma renin activity (1.85 ng/mL/hr) resulted in an increased aldosterone/renin ratio, which was suggestive of primary hyperaldosteronism (PHA). To further support the diagnosis of PHA, the urinary aldosterone/creatinine ratio was calculated both before and after oral administration of fludrocortisone acetate (0.05 mg/kg q 12 hr for 4 consecutive days). The urinary aldosterone/creatinine ratio was 92.6 × 10(-9) before fludrocortisone administration and 155.8 × 10(-9) 4 days later. Absence of suppression was typical of PHA. The cat had a limited response to antihypertensive medication and died before treatment for PHA could be instituted. A necropsy was not permitted by the owner.


Assuntos
Aldosterona/urina , Anti-Inflamatórios/farmacologia , Doenças do Gato/diagnóstico , Creatinina/urina , Fludrocortisona/farmacologia , Hiperaldosteronismo/veterinária , Animais , Doenças do Gato/urina , Gatos , Evolução Fatal , Hiperaldosteronismo/diagnóstico , Hiperaldosteronismo/urina , Masculino
7.
Vet Microbiol ; 177(1-2): 224-7, 2015 May 15.
Artigo em Inglês | MEDLINE | ID: mdl-25770893

RESUMO

Evidence-based information of a cause-and-effect relationship between Ehrlichia canis infection and polyarthritis in naturally- or experimentally-infected dogs is currently lacking. The aim of this prospective study was to investigate whether synovial fluid cytological evidence of arthritis could be documented in dogs with acute monocytic ehrlichiosis. Direct synovial fluid cytology smears from eight Beagle dogs experimentally infected with E. canis were examined prior to, and on 21, 35 and 63 days post-inoculation. The cytological variables assessed included cellularity, percentages of mononuclear cells and neutrophils, macrophage reactivity and evidence of E. canis morulae. The median cellularity and percentages of mononuclear cells and neutrophils prior to inoculation did not differ when compared to post-inoculation cytological evaluation. Increased cellularity, E. canis morulae or cytological evidence of arthritis or macrophage reactivity were not observed throughout the course of the study. In the present study, no cytological evidence of arthritis was found in dogs with experimental acute canine monocytic ehrlichiosis, suggesting that E. canis infection should be considered a rather uncommon cause of arthritis in dogs.


Assuntos
Artrite/veterinária , Doenças do Cão/microbiologia , Doenças do Cão/patologia , Ehrlichia canis , Ehrlichiose/veterinária , Líquido Sinovial/citologia , Animais , Artrite/patologia , Cães , Ehrlichiose/patologia , Monócitos/patologia , Estudos Prospectivos
8.
Vet Microbiol ; 174(1-2): 272-5, 2014 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-25258172

RESUMO

Recent evidence suggest that Bartonella species may cause polyarthritis and lameness in dogs. Canine leishmaniosis (CanL) due to Leishmania infantum is a multi-systemic disease often occurring in association with arthritis. We hypothesized that concurrent Bartonella infection may be a contributing factor for the development of arthritis in dogs with CanL. Hence the primary objective of this study was to investigate the molecular prevalence of Bartonella spp. in dogs with naturally occurring CanL, with or without cytologically documented arthritis. Thirty-eight dogs with CanL (31 with neutrophilic arthritis and 7 without arthritis) were retrospectively studied. Seventy-four archived clinical specimens from these 38 dogs, including 33 blood samples, 19 bone marrow (BM) samples and synovial fluid (SF) aspirates from 22 dogs were tested for Bartonella spp. DNA using the Bartonella alpha proteobacteria growth medium (BAPGM) diagnostic platform. Overall, eight (21.1%) dogs were infected with one or two Bartonella species; however, Bartonella spp. infection was not associated with arthritis in dogs with CanL. Further prospective studies are warranted to determine if there is a correlation between Bartonella spp. infection and the development of arthritis in dogs with CanL.


Assuntos
Artrite/veterinária , Infecções por Bartonella/veterinária , Coinfecção/veterinária , Doenças do Cão , Leishmaniose Visceral/veterinária , Animais , Artrite/microbiologia , Artrite/patologia , Infecções por Bartonella/epidemiologia , Coinfecção/microbiologia , Coinfecção/parasitologia , Meios de Cultura , Primers do DNA/genética , Doenças do Cão/epidemiologia , Doenças do Cão/microbiologia , Doenças do Cão/parasitologia , Cães , Leishmania infantum/isolamento & purificação , Reação em Cadeia da Polimerase/veterinária , Prevalência , Estudos Retrospectivos
9.
Vet Microbiol ; 169(3-4): 198-202, 2014 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-24530039

RESUMO

Ehrlichia canis infection causes multisystemic disease in dogs (canine monocytic ehrlichiosis, CME) which is associated with variable morbidity and mortality. Atypical clinical manifestations, including gastrointestinal signs, may occasionally occur in CME and approximately 10-15% of dogs are presented with historical or clinical evidence of vomiting, diarrhea, and/or abdominal discomfort. The objective of this study was to investigate if there are any alterations in serum canine pancreatic lipase immunoreactivity (cPLI) in dogs with experimentally induced or naturally occurring monocytic ehrlichiosis. Serum samples from 10 Beagle dogs experimentally infected with E. canis and two healthy uninfected Beagles were serially examined; samples from 20 naturally infected dogs (10 with non-myelosuppressive [NME] and 10 with myelosuppressive [ME] ehrlichiosis) were also examined at a given point in time (cross-sectional sampling). None of the experimentally infected Beagles showed gastrointestinal signs or increased cPLI concentrations prior to or following the artificial infection. Three naturally infected dogs with NME and one with ME demonstrated serum cPLI concentrations in the diagnostic range for pancreatitis (>400 µg/L) without showing gastrointestinal signs. The results of the present study indicated that 4/20 (20%) of dogs naturally infected with E. canis demonstrated increased serum cPLI concentrations consistent with mild and clinically inapparent pancreatitis.


Assuntos
Doenças do Cão/enzimologia , Ehrlichiose/veterinária , Lipase/sangue , Lipase/metabolismo , Animais , Doenças do Cão/sangue , Doenças do Cão/diagnóstico , Cães , Ehrlichia canis/imunologia , Ehrlichiose/sangue , Ehrlichiose/complicações , Ehrlichiose/enzimologia , Feminino , Masculino , Monócitos/parasitologia , Pancreatite/etiologia
10.
J Antimicrob Chemother ; 68(7): 1619-26, 2013 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-23475646

RESUMO

OBJECTIVES: To assess the efficacy of rifampicin in achieving clinical and haematological recovery and clearing infection in dogs with experimentally induced acute monocytic ehrlichiosis. METHODS: Five Ehrlichia canis-infected Beagle dogs were treated with rifampicin (10 mg/kg/24 h orally for 3 weeks), nine E. canis-infected dogs received no treatment (infected untreated dogs) and two dogs served as uninfected controls. Clinical score, platelet counts, immunofluorescent antibody titres and PCR detection of E. canis-specific DNA in blood, bone marrow and spleen aspirates were evaluated on post-inoculation days 21 (start of rifampicin), 42 (end of rifampicin) and 98 (end of the study). RESULTS: By day 21 post-inoculation, all infected dogs became clinically ill and thrombocytopenic, seroconverted and were PCR positive in at least one tissue. Clinical scores and antibody titres did not differ between the treated and infected untreated dogs throughout the study. The rifampicin-treated dogs experienced an earlier resolution of their thrombocytopenia (Kaplan-Meier survival plot, P=0.048), and the median platelet counts were significantly higher in the treated compared with the infected untreated dogs on post-inoculation days 42 (P=0.0233) and 98 (P=0.0195). At the end of the study, three treated and six untreated infected dogs remained PCR positive in one tissue each. CONCLUSIONS: The rifampicin treatment regimen applied in this study hastened haematological recovery, but was inconsistent in eliminating the acute E. canis infection.


Assuntos
Antibacterianos/administração & dosagem , Doenças do Cão/tratamento farmacológico , Ehrlichiose/tratamento farmacológico , Rifampina/administração & dosagem , Animais , Carga Bacteriana , Sangue/microbiologia , Medula Óssea/microbiologia , Modelos Animais de Doenças , Doenças do Cão/microbiologia , Doenças do Cão/patologia , Cães , Ehrlichia canis/isolamento & purificação , Ehrlichiose/microbiologia , Ehrlichiose/patologia , Feminino , Masculino , Índice de Gravidade de Doença , Baço/microbiologia , Resultado do Tratamento
11.
Vet Clin Pathol ; 40(1): 78-83, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-21291487

RESUMO

BACKGROUND: Recognition of different cytologic patterns in lymph nodes (LNs) from dogs with canine monocytic ehrlichiosis (CME) and noninfectious causes of lymphoid reactivity may have diagnostic utility. OBJECTIVES: The aims of the present study were to compare cytologic patterns in LNs of dogs with different phases of CME, to investigate the association of cytologic pattern and presence of Ehrlichia spp. morulae, and to compare patterns of lymphoid reactivity between dogs with CME and those with noninfectious causes of lymphoid hyperplasia. METHODS: Cytologic preparations of LNs from 35 dogs with nonmyelosuppressive CME (group A), 16 dogs with myelosuppressive CME (group B), 26 dogs with noninfectious diseases (group C), and 15 healthy dogs (group D) were evaluated. Percentages of lymphocyte types, plasma cells, macrophages, neutrophils, and eosinophils were determined. Samples from dogs in groups A and B were evaluated for the presence of morulae. RESULTS: Cytologic abnormalities in LNs were recorded in 54% of dogs in group A, 88% in group B, 39% in group C, and 0% in group D and were more frequent (P=.02) in dogs with myelosuppressive CME than those with nonmyelosuppressive CME. Plasma cell hyperplasia was more frequent in CME than in noninfectious diseases (P=.03). An association between the presence of cytologic abnormalities and morulae in group A dogs was not found. CONCLUSIONS: Dogs with myelosuppressive CME have more lymphoid cytologic abnormalities than dogs with nonmyelosuppressive CME. LN plasmacytosis is the major pattern of lymphadenopathy in dogs with CME and is found more frequently in dogs with CME than in dogs with noninfectious causes of lymphadenopathy.


Assuntos
Doenças do Cão/patologia , Ehrlichiose/veterinária , Animais , Biópsia por Agulha Fina/veterinária , Estudos de Casos e Controles , Cães , Ehrlichia canis , Ehrlichiose/patologia , Feminino , Linfonodos/citologia , Linfonodos/patologia , Doenças Linfáticas/patologia , Doenças Linfáticas/veterinária , Linfócitos/patologia , Masculino , Monócitos/patologia , Plasmócitos/patologia , Estudos Retrospectivos
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA