Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 822
Filtrar
1.
Nanotechnology ; 2021 Apr 22.
Artigo em Inglês | MEDLINE | ID: mdl-33887703

RESUMO

Electrocatalytic performance of low-cost graphitic carbon nitride (CN) is greatly limited by its limited conductivity and small specific surface area. Herein, a simple and cost-effective idea to produce novel nanocomposite is constructed by the CN and cetyl trimethyl ammonium bromide functionalized carbon black (CB) anchored platinum nanoparticles as highly efficient oxygen reduction catalysts based on gamma irradiation. The assembled carbon nitride/positive carbon black anchoring PtNPs (Pt/CN2-CB+1) catalyst exhibits significantly improved specific surface area, high graphitization, and uniformly dispersed ultra-small platinum nanoparticles. For the oxygen reduction reaction (ORR) performance, the catalyst shows more positive onset-potential (0.93 V vs RHE) and larger diffusion limiting current density (5.65 mA cm-2) compared with benchmark Pt/C catalysts in alkaline medium. Moreover, the Pt/CN2-CB+1 catalyst exhibits a small Tafel slope (92 mV dec-1). Besides, the catalyst was demonstrated the remarkable methanol tolerance and good long-term stability under working conditions. This work provides a new and effective γ-rays irradiation for synthesizing the carbon nitride catalysts for energy conversion and storage applications.

2.
Int J Biol Macromol ; 182: 512-523, 2021 Apr 10.
Artigo em Inglês | MEDLINE | ID: mdl-33848546

RESUMO

Wound dressing composed of polyelectrolyte complexes (PECs), based on chitosan/alginate/hyaluronic acid (CS/ALG/HYA) crosslinked by genipin, was prepared by freeze-dried molding. Genipin as excellent natural biological crosslinker was chose for high biocompatibility and improving mechanical properties of materials. The CS/ALG/HYA sponges (CAHSs) were characterized by FTIR, XRD, DSC and SEM. Porosity, swelling behavior and mechanical properties and in vitro degradation of CAHSs were investigated. The cytotoxicity assay was carried out on HUVEC cells in vitro and the result proves the good biocompatibility of CAHSs. Hemolysis tests indicated that the prepared CAHSs were non-hemolytic material (hemolysis ratio < 5%, no cytotoxicity). PT and aPPT coagulation tests demonstrated that CAHS2 and CAHS3 could both activate the extrinsic and intrinsic coagulation pathway and thus accelerated blood coagulation. Further, in a rat full-thickness wounds model, the CAHS2 sponge significantly facilitates wound closure compared to other groups. CAHSs exhibited adjustable physical, mechanical and biological properties. Thus, the chitosan-based polyelectrolyte composite sponges exhibit great potential as promising wound dressings.

3.
Carbohydr Polym ; 264: 118028, 2021 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-33910719

RESUMO

Serial hemostatic sponges consisting of polysaccharides-modified chitosan foam sponges were prepared by Schiff base crosslinking reaction between the deacetylated chitosan and oxidized dialdehyde cellulose. Such composite foam sponges were characterized by scanning electron microscopy and Fourier-transform infrared spectroscopy to confirm their morphology and compositions. Then the coagulation process was evaluated in vitro by thrombus elasticity meters. Furthermore, the hemostasis experiments on mouse tail vein and rabbit femoral artery were also performed in vivo. The results strongly indicated that such synergistic cellulose-modified chitosan foam sponges showed comprehensively excellent water-absorbing quality, improved mechanical performance, low hemolysis rates, benign cytotoxicity, good resilience ability after repeated compression, and superior hemostasis capability both in vitro and in vivo. Furthermore, the hemostatic mechanism is via adhering/activating the red blood cell/platelet to form robust blood clots through the endogenous coagulation pathway, which serves as a good candidate for emergency trauma treatment in daily civilian and military hemostasis.

4.
Materials (Basel) ; 14(5)2021 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-33803126

RESUMO

The application of electric arc furnace oxidizing slag (EAS) in high strength concrete (HSC) as the cementitious material is investigated in this study. The microstructure and mechanical properties of HSC with four different replacement ratios of EAS were evaluated and HSC with two replacement ratios of ground granulated blast furnace slag (GBS) was used for performance comparison. The results show that the HSC with EAS replacement ratios smaller than 15% undergo similar hydration processes and result in a similar final product when compared with those of NC-NN. Increases in EAS replacement ratio cause a reduction in Ca(OH)2 content; this, in turn, leads to an increase in porosity and a reduction in compressive strength. In terms of shrinkage behavior under free conditions, mixtures with increasing replacement ratios of cementitious materials saw increasing shrinkage, with the HSC containing EAS being similar to the other specimens. The mixtures containing EAS saw a quite gradual decrease in their freezing and thawing resistance properties as the number of freeze-thaw cycles they underwent increased. However, the efficacy of HSC with less than 15% of EAS is similar to GBS; hence, EAS could replace cement in concrete for certain applications, which would lead to more environmental benefits.

5.
Zootaxa ; 4949(2): zootaxa.4949.2.9, 2021 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-33903349

RESUMO

A new armoured scale insect (Hemiptera: Coccomorpha: Diaspididae) species, Nikkoaspis brevispina sp. n., is described and illustrated; it was found on Sinarundiaria sp. (Poaceae; Bambusoideae) in Guizhou Province, China. The type specimens of N. brevispina are deposited at the Institute of Entomology, Guizhou University, Guiyang, China. An identification key based on adult females is provided to separate all eight species in the genus.

6.
Proc Natl Acad Sci U S A ; 118(16)2021 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-33846246

RESUMO

The high northern latitudes (>50°) experienced a pronounced surface stilling (i.e., decline in winds) with climate change. As a drying factor, the influences of changes in winds on the date of autumn foliar senescence (DFS) remain largely unknown and are potentially important as a mechanism explaining the interannual variability of autumn phenology. Using 183,448 phenological observations at 2,405 sites, long-term site-scale water vapor and carbon dioxide flux measurements, and 34 y of satellite greenness data, here we show that the decline in winds is significantly associated with extended DFS and could have a relative importance comparable with temperature and precipitation effects in contributing to the DFS trends. We further demonstrate that decline in winds reduces evapotranspiration, which results in less soil water losses and consequently more favorable growth conditions in late autumn. In addition, declining winds also lead to less leaf abscission damage which could delay leaf senescence and to a decreased cooling effect and therefore less frost damage. Our results are potentially useful for carbon flux modeling because an improved algorithm based on these findings projected overall widespread earlier DFS than currently expected by the end of this century, contributing potentially to a positive feedback to climate.

7.
Int J Pharm ; 601: 120525, 2021 Mar 27.
Artigo em Inglês | MEDLINE | ID: mdl-33781878

RESUMO

Preparing wound dressing with dual-delivery of antioxidant and antibacterial agents is highly desirable in clinical wound treatment. Herein, a series of coaxial nanofiber membranes loaded with antioxidant tea polyphenols (TP) in the core and antibacterial ε-poly (L-lysine) (ε-PL) in the shell layer were successfully fabricated by coaxial electrospinning. The physicochemical characterizations by transmission electron microscopy, inverted fluorescence microscopy and fourier transform infrared spectroscopy confirmed the formation of core-shell structure. The results of in vitro drug release indicated that ε-PL exhibited a fast release profile while TP released in a sustained manner, which is favorable to the achievement of quick bacteria inhibition in the initial phase as well as long-term antioxidant activity during wound healing. The antioxidant activity of coaxial nanofibers was found to be increased with the increment of TP content and incubation time. The antibacterial assays against Escherichia coli and Staphylococcus aureus demonstrated that the incorporation of ε-PL in the coaxial nanofibers led to strong antibacterial activity. Additionally, all the coaxial nanofibers possessed good cytocompatibility. Therefore, the prepared coaxial nanofibers simultaneously incorporated with ε-PL and TP are promising as potential wound dressing materials.

8.
Catheter Cardiovasc Interv ; 97 Suppl 2: 1048-1054, 2021 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-33742738

RESUMO

OBJECTIVES: This study aimed to evaluate prognostic value of quantitative flow ratio (QFR) in drug-coated balloon (DCB) angioplasty for in-stent restenosis (ISR). BACKGROUND: There is a high incidence of recurrent ISR after DCB angioplasty. QFR is a novel method for fast computation of fractional flow reserve for the target vessel based on quantitative coronary angiography (QCA) and fluid dynamics algorithms. METHODS: Patients participating in the RESTORE ISR China randomized trial were enrolled and classified into the recurrent restenosis group and the non-recurrent restenosis group. The binary classifications followed the QCA standards of ISR. Clinical and angiographic characteristics of the groups were analyzed, and the QFRs before and after lesion preparation and after final DCB angioplasty were measured and compared. RESULTS: A total of 208 patients who underwent follow-up angiography were enrolled in the study, with 226 lesions measured in total. QFR value after DCB angioplasty (odds ratio [OR] 0.88; 95% confidence interval [CI] 0.83-0.93; p < .0001 for 1 mm increase), lesion length (OR: 1.08; 95% CI: 1.01-1.15; p = .017), and vessel caliber lumen diameter (OR: 0.35; 95% CI 0.13-0.89; p = .027) were independently associated with recurrent restenosis after DCB angioplasty. The optimal QFR cut-off value was determined to be 0.90 with a sensitivity of 0.94, specificity of 0.56, and accuracy of 0.79 in predicting recurrent restenosis. CONCLUSIONS: The QFR value after DCB angioplasty is a promising predictor of DES ISR.

9.
IEEE Comput Graph Appl ; PP2021 Mar 31.
Artigo em Inglês | MEDLINE | ID: mdl-33788681

RESUMO

The skeleton, or medial axis, is an important attribute of 2D shapes. The disk B-spline curve (DBSC) is a skeleton-based parametric freeform 2D region representation, which is defined in B-spline form. The DBSC describes not only a 2D region, which is suitable for describing heterogonous materials in the region, but also the center curve (skeleton) of the region explicitly, which is suitable for animation, simulation and recognition. In addition to being useful for error estimation of the B-spline curve, the DBSC can be used in designing and animating freeform 2D regions. Despite increasing DBSC applications, its theory and fundamentals have not been thoroughly investigated. In this paper, we discuss several fundamental properties and algorithms, such as the de Boor algorithm for DBSCs. We first derive the explicit evaluation and derivatives formulas at arbitrary points of a 2D region (interior and boundary) represented by a DBSC and then provide heterogeneous object representation. We also introduce modeling and interactive heterogeneous object design methods for a DBSC, which consolidates DBSC theory and supports its further applications.

10.
Exp Biol Med (Maywood) ; : 1535370221997071, 2021 Mar 09.
Artigo em Inglês | MEDLINE | ID: mdl-33715531

RESUMO

This study aims to examine whether miR-31 promotes endogenous NSC proliferation and be used for spinal cord injury management. In the present study, the morpholino knockdown of miR-31 induced abnormal neuronal apoptosis in zebrafish, resulting in impaired development of the tail. miR-31 agomir transfection in NSCs increased Nestin expression and decreased ChAT and GFAP expression levels. miR-31 induced the proliferation of mouse NSCs by upregulating the Notch signaling pathway, and more NSCs entered G1; Notch was inhibited by miR-31 inactivation. Injection of a miR-31 agomir into mouse models of spinal cord injury could effectively restore motor functions after spinal cord injury, which was achieved by promoting the proliferation of endogenous NSCs. After the injection of a miR-31 agomir in spinal cord injury mice, the expression of Nestin and GFAP increased, while GFAP expression decreased. In conclusion, the zebrafish experiments prove that a lack of miR-31 will block nervous system development. In spinal cord injury mouse models, miR-31 overexpression might promote spinal cord injury repair.

11.
Medicine (Baltimore) ; 100(7): e24831, 2021 Feb 19.
Artigo em Inglês | MEDLINE | ID: mdl-33607849

RESUMO

BACKGROUND: Alpha B-crystallin (CRYAB), as a small heat shock protein, may play critical roles in the tumorigenesis and progression of several kinds of human cancers. However, the prognostic value of CRYAB in solid malignancies remains controversial. The aim of the present study was to investigate the association between CRYAB expression and clinicopathology and prognosis of solid tumor patients. METHODS: PubMed, Web of Science, EMBASE, Chinese National Knowledge Infrastructure, and WanFang databases were systematically searched to retrieve studies that investigated the prognostic value of CRYAB expression in various solid tumors. Hazard ratios (HRs) with 95% confidence intervals (CIs) were calculated to determine the strength of association between CRYAB expression and survival in patients with solid tumors. Odds ratios (ORs) with 95% CIs were pooled to assess the correlation between CRYAB expression and clinicopathological characteristics of patients with solid tumors. RESULTS: A total of 17 studies, including 18 cohorts with 6000 patients, were included in this meta-analysis. Our results showed that increased CRYAB expression could predict poor overall survival (HR = 1.81, 95% CI: 1.50-2.19, P < .001), disease-free survival (HR = 1.47, 95% CI: 1.16-1.86, P = .001), and disease-specific survival (HR = 1.40, 95% CI: 1.19-1.63, P < .001) in patients with cancer. Furthermore, the high expression level of CRYAB was associated with certain phenotypes of tumor aggressiveness, such as lymph node metastasis (OR = 2.46, 95% CI: 1.48-4.11, P = .001), distant metastasis (OR = 3.34, 95% CI: 1.96-5.70, P < .001), advanced clinical stage (OR = 2.24, 95% CI: 1.24-4.08, P = .008), low OS rate (OR = 4.81, 95% CI: 2.82-8.19, P < .001), and high recurrence rate (OR = 1.38, 95% CI: 1.11-1.72, P = .004). CONCLUSIONS: CRYAB may serve as a valuable prognostic biomarker and therapeutic target in human solid tumors.


Assuntos
Neoplasias/metabolismo , Cadeia B de alfa-Cristalina/metabolismo , Biomarcadores Tumorais/metabolismo , Carcinogênese/genética , Bases de Dados Factuais , Progressão da Doença , Intervalo Livre de Doença , Feminino , Humanos , Metástase Linfática/patologia , Masculino , Invasividade Neoplásica/genética , Estadiamento de Neoplasias/métodos , Neoplasias/mortalidade , Neoplasias/patologia , Fenótipo , Prognóstico , Recidiva
13.
J Asian Nat Prod Res ; : 1-6, 2021 Feb 15.
Artigo em Inglês | MEDLINE | ID: mdl-33583282

RESUMO

Phytochemical investigation on the roots of Aconitum pseudostapfianum resulted in the isolation of three new aconitine-type C19-diterpenoid alkaloids, pseudostapines A-C (1-3). Their structures were determined by spectral methods such as 1D and 2D (1H-1H COSY, HMQC, NOESY and HMBC) NMR spectroscopy, in addition to high resolution mass spectrometry. The isolated alkaloids were tested in vivo for their antinociceptive potential. As a result, pseudostapine C (3) showed 2-fold more potent antinociceptive effect (ID50 = 60.3 µmol/kg) than the positive control drugs aspirin and acetaminophen.

14.
Life Sci ; 270: 119143, 2021 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-33539913

RESUMO

Osteoarthritis (OA) is the joint pain and dysfunction syndrome caused by severe joint degeneration. The overproduced inflammatory mediators contribute greatly to OA development. It is reported that long non-coding RNA (lncRNA) takes part in many inflammatory diseases. Here, we mainly explored the function of lncRNA SNHG14 in OA process and its specific mechanisms. An OA rat model was induced by destabilizing the medial meniscus (DMM) and IL-1ß (5 ng/mL) was used to mediate an OA cell model in particular chondrocytes (AC). Gain- or loss-of functional assays of SNHG14 and miR-124-3p were carried out to explore their roles in OA development. The experimental statistics illustrated that lncRNA SNHG14 and IL-1ß mRNA expression were both increased in OA tissues, while miR-124-3p was lowly-expressed. Linear regression analysis showed that SNHG14 and miR-124-3p had negative relationship in the OA tissues. In the in vitro experiments, downregulation of lncRNA SNHG14 promoted the proliferation of IL-1ß-treated AC and inhibited cell apoptosis and COX-2, iNOS, TNF-α, IL-6 expression. Moreover, lncRNA SNHG14 inhibited miR-124-3p expression as a miRNA sponge. MiR-124-3p targeted the 3'non-translated region (3'UTR) of FSTL-1 and TLR4 and inhibited their expressions. Also, the in vivo experiments confirmed that knocking down SNHG14 relieved the progression of OA in rats via inhibiting inflammatory responses. In conclusion, this study confirmed that downregulation of lncRNA SNHG14 inhibits FSTL-1-mediated activation of NLRP3 and TLR4/NF-κB signalling pathway activation by targeting miR-124-3p, thus attenuating inflammatory reactions in OA.


Assuntos
MicroRNAs/genética , Osteoartrite/genética , RNA Longo não Codificante/genética , Adulto , Animais , Apoptose , China , Condrócitos/metabolismo , Feminino , Proteínas Relacionadas à Folistatina/genética , Proteínas Relacionadas à Folistatina/metabolismo , Humanos , Inflamação/metabolismo , Interleucina-1beta , Masculino , MicroRNAs/metabolismo , Pessoa de Meia-Idade , NF-kappa B/metabolismo , Proteína 3 que Contém Domínio de Pirina da Família NLR/metabolismo , Osteoartrite/metabolismo , RNA Longo não Codificante/metabolismo , Ratos , Ratos Sprague-Dawley , Transdução de Sinais , Receptor 4 Toll-Like/metabolismo
15.
PLoS One ; 16(2): e0243792, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33539348

RESUMO

BACKGROUND: The association between personal hair dye use and breast cancer risk is currently debated. The aim of this work is to investigate the association between the use of hair care products and breast cancer risk in women. METHODS: Based on the PRISMA-IPD statement, the PubMed, Embase, Cochrane Library, Web of Science, OVID and Scopus databases were used to identify eligible studies published from inception to 22 April 2020. A pooled odds ratio (OR) with a 95% confidential interval (CI) was calculated to assess this correlation via fixed- or random-effect Mantel-Haenszel models using a heterogeneity Chi2 test with a significance level of p<0.1. All statistical tests were performed using StataSE software (version 12.0). RESULTS: The analyzed data comprised 14 eligible studies with 210319 unique subjects. The pooled results suggested that there was a significant association between the use of hair dyes and breast cancer occurrence (pooled OR = 1.07; 95% CI, 1.01-1.13). Regarding the individual analysis regarding the different types of hair chemicals, permanent hair dye users (pooled OR = 1.08; 95% CI, 1.03-1.14) and rinse users (pooled OR = 1.17; 95% CI, 1.02-1.35) were both found to have a significantly elevated breast cancer risk compared to natural hair subjects, whereas there was an insignificant relationship between the use of semipermanent hair dyes (pooled OR = 1.09; 95% CI, 0.92-1.28) and straighteners (pooled OR = 1.04; 95% CI, 0.96-1.14) and breast cancer risk. No impact on the overall correlation between hair dyes and breast cancer risk due to race (White vs non-White) (pooled OR = 1.05; 95% CI, 0.86-1.29), timing of use (<10 years vs ≥10 years) (pooled OR = 0.96; 95% CI, 0.85-1.08) or dye color (Darker than natural hair vs Lighter than natural hair) (pooled OR = 0.91; 95% CI, 0.62-1.32) was found. CONCLUSIONS: Chemicals in hair dyes may play a role in breast carcinogenesis and increase breast cancer risk.

16.
Arch Gynecol Obstet ; 2021 Feb 24.
Artigo em Inglês | MEDLINE | ID: mdl-33625596

RESUMO

PURPOSE: Vitamin D (VD) deficiency seems to be associated with the risk of recurrent spontaneous abortion (RSA). Vitamin D receptor (VDR) and cytochrome P450 family 2 subfamily R member 1 (CYP2R1) are two genes which are vital for VD metabolism and actions. However, whether single-nucleotide polymorphisms (SNPs) in these genes are correlated with the risk of RSA are poorly understood. Therefore, we aimed to characterize the relationships among VDR SNPs, CYP2R1 SNPs and RSA. METHODS: This case-control study enrolled 75 RSA patients and 83 controls. Serum VD and some cytokines were detected with LC-MS/MS and flow cytometry, respectively. Genotyping for three SNPs of CYP2R1 (rs10741657, rs10766197 and rs12794714) and five SNPs of VDR (rs7975232, rs1544410, rs2189480, rs2228570 and rs2239179) was done with polymerase chain reaction (PCR) and high-throughput sequencing. All the data were analyzed with appropriate methods and in different models. RESULTS: The results revealed a significant correlation between the AG genotype of CYP2R1 rs12794714 and VD levels (OR 0.686; 95% CI 0.49-0.96; p = 0.028). Besides, the AG and GG genotypes of CYP2R1 rs12794714 were markedly related to the risk of RSA (OR 52.394, 59.497; 95% CI 2.683-1023.265, 3.110-1138.367; p = 0.009, 0.007, respectively). CONCLUSION: Our results indicate that CYP2R1 rs12794714 might be a risk factor for RSA. Hence, early screening of pregnant women for CYP2R1 rs12794714 is necessary to warrant proactive counseling and treatment against RSA.

17.
J Cell Mol Med ; 25(5): 2426-2435, 2021 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-33512770

RESUMO

The aim of the present study was to explore the potential mechanism underlying the involvement of CB2 in osteoporosis. Micro-CT was utilized to examine femur bone architecture. Also, real-time PCR and Western blot analysis were utilized to detect the effect of 2-AG on the expression of CB2 and Notch, or the interaction between CB2 and Notch 2. 2-AG treatment up-regulated BMD, Tb.Sp and SMI in OVX mice, whereas proportion of bone volume in total volume (BV/TV), trabecular thickness (Tb.Th), trabecular number (Tb.N) and bone mineral density (BMD) were decreased in 2-AG-treated OVX mice. Accordingly, 2-AG administration up-regulated Notch 1 expression in OVX mice but had no effect on CB2 and Notch 2 expression. Meanwhile, 2-AG administration promoted the differentiation of hBMSCs in OVX mice, while exhibiting no effect on the proliferation of hBMSCs. Furthermore, in the cellular models, 2-AG treatment also up-regulated Notch 1 expression but had no effect on CB2 and Notch 2 expression, while Notch 1 shRNA had no effect on CB2 and Notch 2 expression. 2-AG promoted cell proliferation and differentiation, which were inhibited by Notch 1 shRNA. NICD had no effect on CB2 level but increased Notch 1 expression, and CB2 shRNA decreased CB2 and Notch 1 expression. Finally, CB2 shRNA inhibited cell proliferation and differentiation, whereas NICD promoted proliferation and differentiation of hBMSCs. Our results provided further evidence for the association of CB2 gene with BMD and osteoporosis, and identified CB2 as a promising target for the treatment of osteoporosis.

18.
J Med Internet Res ; 23(1): e19928, 2021 01 06.
Artigo em Inglês | MEDLINE | ID: mdl-33404508

RESUMO

BACKGROUND: Artificial intelligence (AI)-driven chatbots are increasingly being used in health care, but most chatbots are designed for a specific population and evaluated in controlled settings. There is little research documenting how health consumers (eg, patients and caregivers) use chatbots for self-diagnosis purposes in real-world scenarios. OBJECTIVE: The aim of this research was to understand how health chatbots are used in a real-world context, what issues and barriers exist in their usage, and how the user experience of this novel technology can be improved. METHODS: We employed a data-driven approach to analyze the system log of a widely deployed self-diagnosis chatbot in China. Our data set consisted of 47,684 consultation sessions initiated by 16,519 users over 6 months. The log data included a variety of information, including users' nonidentifiable demographic information, consultation details, diagnostic reports, and user feedback. We conducted both statistical analysis and content analysis on this heterogeneous data set. RESULTS: The chatbot users spanned all age groups, including middle-aged and older adults. Users consulted the chatbot on a wide range of medical conditions, including those that often entail considerable privacy and social stigma issues. Furthermore, we distilled 2 prominent issues in the use of the chatbot: (1) a considerable number of users dropped out in the middle of their consultation sessions, and (2) some users pretended to have health concerns and used the chatbot for nontherapeutic purposes. Finally, we identified a set of user concerns regarding the use of the chatbot, including insufficient actionable information and perceived inaccurate diagnostic suggestions. CONCLUSIONS: Although health chatbots are considered to be convenient tools for enhancing patient-centered care, there are issues and barriers impeding the optimal use of this novel technology. Designers and developers should employ user-centered approaches to address the issues and user concerns to achieve the best uptake and utilization. We conclude the paper by discussing several design implications, including making the chatbots more informative, easy-to-use, and trustworthy, as well as improving the onboarding experience to enhance user engagement.


Assuntos
Inteligência Artificial/normas , Telemedicina/métodos , Adulto , Feminino , Humanos , Masculino , Projetos de Pesquisa , Mídias Sociais
19.
Theranostics ; 11(2): 506-521, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33391489

RESUMO

Inflammation is a pivotal driver of atherosclerotic plaque progression and rupture and is a target for identifying vulnerable plaques. However, challenges arise with the current in vivo imaging modalities for differentiating vulnerable atherosclerotic plaques from stable plaques due to their low specificity and sensitivity. Herein, we aimed to develop a novel multimodal imaging platform that specifically targets and identifies high-risk plaques in vivo by detecting active myeloperoxidase (MPO), a potential inflammatory marker of vulnerable atherosclerotic plaque. Methods: A novel multimodal imaging agent, 5-HT-Fe3O4-Cy7 nanoparticles (5HFeC NPs), used for active MPO targeting, was designed by conjugating superparamagnetic iron oxide nanoparticles (SPIONs) with 5-hydroxytryptamine and cyanine 7 N-hydroxysuccinimide ester. The specificity and sensitivity of 5HFeC NPs were evaluated using magnetic particle imaging (MPI), fluorescence imaging (FLI), and computed tomographic angiography (CTA) in an ApoE-/- atherosclerosis mouse model. Treatment with 4-ABAH, an MPO inhibitor, was used to assess the monitoring ability of 5HFeC NPs. Results: 5HFeC NPs can sensitively differentiate and accurately localize vulnerable atherosclerotic plaques in ApoE-/- mice via MPI/FLI/CTA. High MPI and FLI signals were observed in atherosclerotic plaques within the abdominal aorta, which were histologically confirmed by multiple high-risk features of macrophage infiltration, neovascularization, and microcalcification. Inhibition of active MPO reduced accumulation of 5HFeC NPs in the abdominal aorta. Accumulation of 5HFeC NPs in plaques enabled quantitative evaluation of the severity of inflammation and monitoring of MPO activity. Conclusions: This multimodal MPI approach revealed that active MPO-targeted nanoparticles might serve as a method for detecting vulnerable atherosclerotic plaques and monitoring MPO activity.

20.
Cell Death Dis ; 12(1): 2, 2021 01 06.
Artigo em Inglês | MEDLINE | ID: mdl-33414463

RESUMO

Lysine (K)-specific demethylase 6B (KDM6B), a stress-inducible H3K27me3 demethylase, plays oncogenic or antitumoral roles in malignant tumors depending on the type of tumor cell. However, how this histone modifier affects the progression of prostate cancer (PCa) is still unknown. Here we analyzed sequenced gene expression data and tissue microarray to explore the expression features and prognostic value of KDM6B in PCa. Further, we performed in vitro cell biological experiments and in vivo nude mouse models to reveal the biological function, upstream and downstream regulation mechanism of KDM6B. In addition, we investigated the effects of a KDM6B inhibitor, GSK-J4, on PCa cells. We showed that KDM6B overexpression was observed in PCa, and elevated KDM6B expression was associated with high Gleason Score, low serum prostate-specific antigen level and shorted recurrence-free survival. Moreover, KDM6B prompted proliferation, migration, invasion and cell cycle progression and suppressed apoptosis in PCa cells. GSK-J4 administration could significantly suppress the biological function of KDM6B in PCa cells. KDM6B is involved in the development of castration-resistant prostate cancer (CRPC), and combination of MDV3100 plus GSK-J4 is effective for CRPC and MDV3100-resistant CRPC. Mechanism exploration revealed that androgen receptor can decrease the transcription of KDM6B and that KDM6B demethylates H3K27me3 at the cyclin D1 promoter and cooperates with smad2/3 to prompt the expression of cyclin D1. In conclusion, our study demonstrates that KDM6B is an androgen receptor regulated gene and plays oncogenic roles by promoting cyclin D1 transcription in PCa and GSK-J4 has the potential to be a promising agent for the treatment of PCa.

SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA
...