Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 35
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
G3 (Bethesda) ; 11(1)2021 Jan 18.
Artigo em Inglês | MEDLINE | ID: mdl-33561235

RESUMO

The swamp eel (Monopterus albus) is one economically important fish in China and South-Eastern Asia and a good model species to study sex inversion. There are different genetic lineages and multiple local strains of swamp eel in China, and one local strain of M. albus with deep yellow and big spots has been selected for consecutive selective breeding due to superiority in growth rate and fecundity. A high-quality reference genome of the swamp eel would be a very useful resource for future selective breeding program. In the present study, we applied PacBio single-molecule sequencing technique (SMRT) and the high-throughput chromosome conformation capture (Hi-C) technologies to assemble the M. albus genome. A 799 Mb genome was obtained with the contig N50 length of 2.4 Mb and scaffold N50 length of 67.24 Mb, indicating 110-fold and ∼31.87-fold improvement compared to the earlier released assembly (∼22.24 Kb and 2.11 Mb, respectively). Aided with Hi-C data, a total of 750 contigs were reliably assembled into 12 chromosomes. Using 22,373 protein-coding genes annotated here, the phylogenetic relationships of the swamp eel with other teleosts showed that swamp eel separated from the common ancestor of Zig-zag eel ∼49.9 million years ago, and 769 gene families were found expanded, which are mainly enriched in the immune system, sensory system, and transport and catabolism. This highly accurate, chromosome-level reference genome of M. albus obtained in this work will be used for the development of genome-scale selective breeding.

2.
ACS Omega ; 5(43): 28212-28223, 2020 Nov 03.
Artigo em Inglês | MEDLINE | ID: mdl-33163804

RESUMO

The CuO-La2O3/ZrO2 catalysts calcined at different temperatures from 500 to 800 °C were studied for the hydrogenation of oxalates to ethylene glycol (EG). Along with the increase of calcination temperatures, the BET surface area, pore volume, and Cu dispersion decreased, whereas the crystallite sizes of Cu species increased. Interestingly, the superior performance such as a 98% selectivity of EG in dimethyl oxalate hydrogenation or a 96.5% selectivity of EG in diethyl oxalate hydrogenation was obtained over the catalyst calcined at 700 °C. Essentially, the surface synergism between Cu species and monoclinic ZrO2 was enhanced by the higher calcination temperature, resulting in the remarkable surface adsorption and activation of H2. Besides, the increase of calcination temperature significantly reduced the surface acidity and basicity, which could effectively suppress the byproduct formation.

3.
Genes (Basel) ; 11(11)2020 Oct 26.
Artigo em Inglês | MEDLINE | ID: mdl-33114541

RESUMO

In fish, sperm motility activation is one of the most essential procedures for fertilization. Previous studies have mainly focused on the external environmental effects and intracellular signals in sperm activation; however, little is known about the metabolic process of sperm motility activation in fish. In the present study, using ricefield eel (Monopterus albus) sperm as a model, metabonomics was used to analyze the metabolic mechanism of the sperm motility activation in fish. Firstly, 529 metabolites were identified in the sperm of ricefield eel, which were clustered into the organic acids, amino acids, nucleotides, benzene, and carbohydrates, respectively. Among them, the most abundant metabolites in sperm were L-phenylalanine, DL-leucine, L-leucine, lysolecithin choline 18:0, L-tryptophan, adenine, hypoxanthine, 7-Methylguanine, shikimic acid, and L-tyrosine. Secondly, compared to pre-activated sperm, the level of S-sulfo-L-cysteine and L-asparagine were both increased in the post-activated sperm. Ninety-two metabolites were decreased in the post-activated sperm, including quinic acid, acetylsalicylic acid, 7,8-dihydro L-biopterin, citric acid, glycylphenylalanine, and dihydrotachysterol (DHT). Finally, basing on the pathway analysis, we found that the changed metabolites in sperm motility activation were mainly clustered into energy metabolism and anti-oxidative stress. Fish sperm motility activation would be accompanied by the release of a large amount of energy, which might damage the genetic material of sperm. Thus, the anti-oxidative stress function is a critical process to maintain the normal physiological function of sperm.

4.
Sensors (Basel) ; 20(17)2020 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-32872219

RESUMO

Accurately quantifying spatiotemporal changes in surface water is essential for water resources management, nevertheless, the dynamics of Poyang Lake surface water areas with high spatiotemporal resolution, as well as its responses to climate change, still face considerable uncertainties. Using the time series of Sentinel-1 images with 6- or 12-day intervals, the Sentinel-1 water index (SWI), and SWI-based water extraction model (SWIM) from 2015 to 2020 were used to document and study the short-term characteristics of southwest Poyang Lake surface water. The results showed that the overall accuracy of surface water area was satisfactory with an average of 91.92%, and the surface water area ranged from 129.06 km2 on 2 March 2017 to 1042.57 km2 on 17 July 2016, with significant intra- and inter-month variability. Within the 6-day interval, the maximum change of lake area was 233.42 km2 (i.e., increasing from 474.70 km2 up to 708.12 km2). We found that the correlation coefficient between the water area and the 45-day accumulated precipitation reached to 0.75 (p < 0.001). Moreover, a prediction model was built to predict the water area based on climate records. These results highlight the significance of high spatiotemporal resolution mapping for surface water in the erratic southwest Poyang Lake under a changing climate. The automated water extraction algorithm proposed in this study has potential applications in delineating surface water dynamics at broad geographic scales.

5.
Environ Technol ; : 1-16, 2020 Jul 31.
Artigo em Inglês | MEDLINE | ID: mdl-32674712

RESUMO

Waste residue of Chinese prickly ash seeds were simply treated with aqueous ZnCl2 to prepared the high-performed activated carbon. It was characterized by the methods of XRD, SEM, EDX, FT-IR, BET and XPS. The synergetic adsorption performance of Chinese prickly ash seeds activated carbon for Pb2+, Ni2+ and Acid Orange IΙ (AO) was studied. In the single-component system, the adsorption capacity of Pb2+, Ni2+ and AO were 15.1, 10.7 and 188.4 mg/g, respectively. In the AO-Pb2+ system, the maximum adsorption capacity of Pb2+ and AO were 79.40 and 332.68 mg/g under temperature of 30°C and pH of 5.0, respectively. For AO-Ni2+ system, it was 375.6 and 38.3 mg/g, respectively. The adsorption kinetics was satisfactorily fitted by the pseudo-second-order model. The synergic adsorption process can be smoothly described by the non-modified Sips isotherm.

6.
Infect Genet Evol ; 84: 104379, 2020 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-32497680

RESUMO

The Ranavirus (one genus of Iridovidae family) is an emerging pathogen that infects fish, amphibian, and reptiles, and causes great economical loss and ecological threat to farmed and wild animals globally. The major capsid protein (MCP) has been used as genetic typing marker and as target to design vaccines. Herein, the codon usage pattern of 73 MCP genes of Ranavirus and Lymphocystivirus are studied by calculating effective number of codons (ENC), relative synonymous codon usage (RSCU), codon adaptation index (CAI), and relative codon deoptimization index (RCDI), and similarity index (SiD). The Ranavirus are confirmed to be classified into five groups by using phylogenetic analysis, and varied nucleotide compositions and hierarchical cluster analysis based on RSCU. The results revealed different codon usage patterns among Lymphocystivirus and five groups of Ranavirus. Ranavirus had six over-represented codons ended with G/C nucleotide, while Lymphocystivirus had six over-represented codons ended with A/T nucleotide. A comparative analysis of parameters that define virus and host relatedness in terms of codon usage were analyzed indicated that Amphibian-like ranaviruses (ALRVs) seem to possess lower ENC values and higher CAIs in contrast to other ranaviruses isolated from fishes, and two groups (FV3-like and CMTV-like group) of them had received higher selection pressure from their hosts as having higher relative codon deoptimization index (RCDI) and similarity index (SiD). The correspondence analysis (COA) and Spearman's rank correlation analyses revealed that nucleotide compositions, relative dinucleotide frequency, mutation pressure, and natural translational selection shape the codon usage pattern in MCP genes and the ENC-GC3S and neutrality plots indicated that the natural selection is the predominant factor. These results contribute to understanding the evolution of Ranavirus and their adaptions to their hosts.

7.
BMC Genomics ; 21(1): 269, 2020 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-32228450

RESUMO

BACKGROUND: The nucleoli, including their proteomes, of higher eukaryotes have been extensively studied, while few studies about the nucleoli of the lower eukaryotes - protists were reported. Giardia lamblia, a protist with the controversy of whether it is an extreme primitive eukaryote or just a highly evolved parasite, might be an interesting object for carrying out the nucleolar proteome study of protists and for further examining the controversy. RESULTS: Using bioinformatics methods, we reconstructed G. lamblia nucleolar proteome (GiNuP) and the common nucleolar proteome of the three representative higher eukaryotes (human, Arabidopsis, yeast) (HEBNuP). Comparisons of the two proteomes revealed that: 1) GiNuP is much smaller than HEBNuP, but 78.4% of its proteins have orthologs in the latter; 2) More than 68% of the GiNuP proteins are involved in the "Ribosome related" function, and the others participate in the other functions, and these two groups of proteins are much larger and much smaller than those in HEBNuP, respectively; 3) Both GiNuP and HEBNuP have their own specific proteins, but HEBNuP has a much higher proportion of such proteins to participate in more categories of nucleolar functions. CONCLUSION: For the first time the nucleolar proteome of a protist - Giardia was reconstructed. The results of comparison of it with the common proteome of three representative higher eukaryotes -- HEBNuP indicated that the simplicity of GiNuP is most probably a reflection of primitiveness but not just parasitic reduction of Giardia, and simultaneously revealed some interesting evolutionary phenomena about the nucleolus and even the eukaryotic cell, compositionally and functionally.

8.
Virology ; 545: 10-15, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32174454

RESUMO

Lassa fever (LF) is a viral hemorrhagic fever that causes high morbidity and severe mortality annually. The disease is endemic to two geographically separate areas within tropical West Africa, one in Nigeria and the second predominantly in Sierra Leone-Guinea-Liberia-Mali. Lassa virus (LASV), the causative agent of the disease, exhibits clear delineation of phylogeography between the endemic areas. In order to characterize the genetic nature of Nigerian-non-Nigerian epidemic split, we performed molecular epidemiological analyses on non-Nigerian isolates (lineage IV as well as lineage V) and their sister group from north-central Nigeria (lineage III). The results showed that adaptive genetic diversification has occurred between these currently circulating clusters in the spread process, and a number of replacement divergences have been fixed between these clusters on the viral RNA-dependent RNA polymerase (L protein). This study highlights the viral L protein could be a determinant factor for the epidemic split.


Assuntos
Febre Lassa/virologia , Vírus Lassa/genética , África Ocidental/epidemiologia , Epidemias , Variação Genética , Humanos , Febre Lassa/epidemiologia , Vírus Lassa/classificação , Vírus Lassa/isolamento & purificação , Nigéria/epidemiologia , Filogenia , Filogeografia , Proteínas Virais/genética
9.
J Environ Manage ; 253: 109695, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31634744

RESUMO

Elemental doping has been proven to be an effective strategy for increasing the catalytic activity and structural stability of Fenton catalysts. Therefore, this work reports that Mn-doped FeS2/Fe2O3 (Mn3O4-FeS2/Fe2O3) has excellent catalytic performance for the degradation of Orange II under simulated solar energy. Degradation experiment results showed that the sample with a manganese-iron molar ratio of 1:2 exhibited higher activity than others. The degradation rate of 20 mg/L OII reached 99.0% in 18 min under the conditions of 0.3 g/L Mn3O4-FeS2/Fe2O3, 5 mM H2O2 and pH = 2.8. In addition to, the Mn3O4-FeS2/Fe2O3 catalyst shows good reusability for Orange II and high activity for other dyes (MB, MG, Rh B and MO) under optimal conditions. Degradation mechanism study indicated that the heterogeneous Fenton reaction was promoted by retarding the recombination of photogenerated charge carriers and accelerating the cycle between Fe3+/Mn2+ and Fe2+/Mn3+, which improved photo-Fenton-like catalytic performance, resulting in the enhanced degradation of organic pollutant. Finally, a possible degradation pathway was proposed according to the results of liquid chromatography-mass spectrometry (LC-MS). In short, the catalyst has potential application value in wastewater treatment.


Assuntos
Compostos Azo , Peróxido de Hidrogênio , Benzenossulfonatos , Catálise
10.
BMC Genomics ; 20(1): 415, 2019 May 23.
Artigo em Inglês | MEDLINE | ID: mdl-31122206

RESUMO

BACKGROUND: Chinese giant salamander Andrias davidianus is an endangered species. The success of artificial breeding provides a useful way to protect this species. However, the method to identify the sex and mechanism of sex determination were unclear which hinder the improvement of the artificial breeding. Detection of a sex specific marker provides an effective approach to identify genetic sex and investigate the sex determination mechanism. RESULTS: We used restriction-site-associated DNA (RAD) sequencing to isolate a sex-specific genetic marker in A. davidianus to expand knowledge of the sex determination mechanism. Four male and four female specimens were subjected to RAD sequencing, which generated 934,072,989 reads containing approximately 134.4 Gb of sequences. The first round of comparison of the assembled sequence against the opposite sex raw reads revealed 19,097 female and 17,994 male unmatched sequences. Subsequently, 19,097 female sequences were subjected to a BLAST search against male genomic data, which revealed 308 sequences unmapped to the male genome. One hundred of these were randomly selected and validated by PCR in five male and five female specimens, and four putative sex-specific sequences were produced. Further validation was performed by PCR in another 24 females and 24 males, and all female individuals exhibited the expected specific bands, while the males did not. To apply the sex-specific marker, three specimens reversed from genetic female to physiological male were found in a group exposed to elevated temperature, and 13 individuals reversed from genetic male to physiological female were obtained in a 17ß-estradiol exposed group. CONCLUSION: This is the first report of a sex-specific marker in A. davidianus and may have potential for elucidation of its sex determination mechanism and, hence, its conservation.


Assuntos
Caracteres Sexuais , Urodelos/genética , Animais , Feminino , Marcadores Genéticos , Genoma , Masculino , Análise de Sequência de DNA
11.
Genomics ; 111(6): 1351-1359, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-30244141

RESUMO

We characterized the Andrias davidianus T-box 1 (Tbx1) gene. Tbx1 expression was high in testis and low in other examined tissues. Immunohistochemistry detected tbx1 expression in somatic and germ cells 62 days post-hatching (dph), prior to gonad differentiation. At 210 dph, after gonad differentiation, tbx1 was expressed in spermatogonia and testis somatic cells and in granulosa cells in ovary. Tbx1 expression was up-regulated in ovary after high temperature treatment. In the neomale, tbx1 expression showed a similar profile to normal males, and vice-versa for genetic male. Over-expression of tbx1 in females after injection of TBX1 protein down-regulated the female-biased genes cyp19a and foxl2 and up-regulated the male-biased amh gene. When tbx1 was knocked down by tbx1/siRNA, cyp19a and foxl2 expression was up-regulated, and expression of amh, cyp26a, dmrt1, and wt1 was down-regulated. Results suggest that tbx1 influenced sex-related gene expression and participates in regulation of A. davidianus testis development.


Assuntos
Proteínas de Anfíbios/metabolismo , Regulação da Expressão Gênica no Desenvolvimento , Proteínas com Domínio T/metabolismo , Transcriptoma , Urodelos/metabolismo , Proteínas de Anfíbios/genética , Animais , Feminino , Hormônios Esteroides Gonadais/farmacologia , Masculino , Ovário/efeitos dos fármacos , Ovário/metabolismo , Filogenia , Diferenciação Sexual/efeitos dos fármacos , Proteínas com Domínio T/genética , Testículo/efeitos dos fármacos , Testículo/metabolismo , Urodelos/genética
12.
Mol Genet Genomics ; 294(2): 287-299, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30377773

RESUMO

The Chinese giant salamander Andrias davidianus is a protected amphibian with high nutritional and economic value. Understanding its sex determination mechanism is important for improving culture techniques and sex control in breeding. However, little information on the characterization of critical genes involved in sex is available. Herein, sequencing of ovary and test produced 40,783,222 and 46,128,902 raw reads, respectively, which were jointly assembled into 80,497 unigenes. Of these, 36,609 unigenes were annotated, of which 8907 were female-biased and 10,385 were male-biased. Several sex-related pathways were observed, including the Wnt signaling pathway. After elevated temperature and estrogen exposure, neomale and neofemale specimens were identified by a female-specific marker for the first time. RT-qPCR analysis showed the expression profile of ten selected sex-biased genes to be exhibited consistently in male and neomale and in female and neofemale, with the exception of the Amh and TfIIIa genes. Results suggested that these genes may play important roles in A. davidianus sex determination and gonad development. This provides a basis for further investigation of the molecular mechanisms of sex determination in amphibians.


Assuntos
Regulação da Expressão Gênica no Desenvolvimento/genética , Processos de Determinação Sexual , Transcriptoma/genética , Urodelos/genética , Animais , Feminino , Gônadas/crescimento & desenvolvimento , Sequenciamento de Nucleotídeos em Larga Escala , Masculino , Anotação de Sequência Molecular , Ovário/crescimento & desenvolvimento , Urodelos/crescimento & desenvolvimento
13.
Biol Open ; 7(11)2018 Nov 13.
Artigo em Inglês | MEDLINE | ID: mdl-30425109

RESUMO

Animal egg coats are composed of different glycoproteins collectively named zona pellucida (ZP) proteins. The characterized vertebrate genes encoding ZP proteins have been classified into six subfamilies, and exhibit low similarity to the ZP genes characterized in certain invertebrates. The origin and evolution of the vertebrate ZP genes remain obscure. A search against 97 representative metazoan species revealed various numbers (ranging from three to 33) of different putative egg-coat ZP genes in all 47 vertebrates and several ZP genes in five invertebrate species, but no putative ZP gene was found in the other 45 species. Based on phylogenetic and synteny analyses, all vertebrate egg-coat ZP genes were classified into eight ZP gene subfamilies. Lineage- and species-specific gene duplications and gene losses occurred frequently and represented the main causes of the patchy distribution of the eight ZP gene subfamilies in vertebrates. Thorough phylogenetic analyses revealed that the vertebrate ZP genes could be traced to three independent origins but were not orthologues of the characterized invertebrate ZP genes. Our results suggested that vertebrate egg-coat ZP genes should be classified into eight subfamilies, and a putative evolutionary map is proposed. These findings would aid the functional and evolutionary analyses of these reproductive genes in vertebrates.

14.
Curr Biol ; 28(10): R592-R594, 2018 05 21.
Artigo em Inglês | MEDLINE | ID: mdl-29787717

RESUMO

Species with large geographic ranges are considered resilient to global decline [1]. However, human pressures on biodiversity affect increasingly large areas, in particular across Asia, where market forces drive overexploitation of species [2]. Range-wide threat assessments are often costly and thus extrapolated from non-representative local studies [3]. The Chinese giant salamander (Andrias davidianus), the world's largest amphibian, is thought to occur across much of China, but populations are harvested for farming as luxury food [4]. Between 2013 and 2016, we conducted field surveys and 2,872 interviews in possibly the largest wildlife survey conducted in China. This extensive effort revealed that populations of this once-widespread species are now critically depleted or extirpated across all surveyed areas of their range, and illegal poaching is widespread.


Assuntos
Conservação dos Recursos Naturais , Extinção Biológica , Urodelos , Animais , China , Densidade Demográfica
15.
Dev Comp Immunol ; 85: 108-114, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29626489

RESUMO

Andrias davidianus is a large and economically important amphibian in China. Ranavirus infection causes serious losses in A. davidianus farming industry. MicroRNA mediated host-pathogen interactions are important in antiviral defense. In this study, five small-RNA libraries from ranavirus infected and non-infected A. davidianus spleens were sequenced using high throughput sequencing. The miRNA expression pattern, potential functions, and target genes were investigated. In total, 1356 known and 431 novel miRNAs were discovered. GO and KEGG analysis revealed that certain miRNA target genes are associated with apoptotic, signal pathway, and immune response categories. Analysis identified 82 downregulated and 9 upregulated differentially expressed miRNAs, whose putative target genes are involved in pattern-recognition receptor signaling pathways and immune response. These findings suggested miRNAs play key roles in A. davidianus's response to ranavirus and could provide a reference for further miRNA functional identification, leading to novel approaches to improve A. davidianus ranavirus resistance.


Assuntos
MicroRNAs/genética , Ranavirus/patogenicidade , Urodelos/genética , Urodelos/virologia , Animais , Apoptose/genética , China , Regulação para Baixo/genética , Sequenciamento de Nucleotídeos em Larga Escala/métodos , Interações Hospedeiro-Patógeno/genética , Imunidade/genética , Proteínas de Membrana/genética , Serina Endopeptidases/genética , Regulação para Cima/genética
16.
Hereditas ; 155: 18, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29483849

RESUMO

To search the evidence of molecular evolution mechanism for aquatic and cave habitat in Andrias davidianus, the evolution analysis was carried out among several species transcriptome data. The transcriptome data of Notophthalmus viridescens, Xenopus tropicalis, Cynops pyrrhogaster, Hynobius chinensis and A. davidianus were obtained from the Genbank and reassembled except Xenopus tropicalis. The BLAST search of transcriptome data obtained 1244 single-copy orthologous genes among five species. A phylogenetic tree showed A. davidianus to have the closest relationship to H. chinensis. Fourteen positively selected genes were detected in A. davidianus and N. vridescens group and fifteen in A. davidianus and H. chinensis group. Five genes were shared in the both groups which involved in the immune system, suggesting that A. davidianus adaptation to an aquatic and cave environment required rapid evolution of the immune system compared to N. viridescens and H. chinensis.


Assuntos
Evolução Molecular , Filogenia , Transcriptoma , Urodelos/genética , Animais
17.
Sensors (Basel) ; 18(1)2018 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-29324647

RESUMO

Areas and spatial distribution information of paddy rice are important for managing food security, water use, and climate change. However, there are many difficulties in mapping paddy rice, especially mapping multi-season paddy rice in rainy regions, including differences in phenology, the influence of weather, and farmland fragmentation. To resolve these problems, a novel multi-season paddy rice mapping approach based on Sentinel-1A and Landsat-8 data is proposed. First, Sentinel-1A data were enhanced based on the fact that the backscattering coefficient of paddy rice varies according to its growth stage. Second, cropland information was enhanced based on the fact that the NDVI of cropland in winter is lower than that in the growing season. Then, paddy rice and cropland areas were extracted using a K-Means unsupervised classifier with enhanced images. Third, to further improve the paddy rice classification accuracy, cropland information was utilized to optimize distribution of paddy rice by the fact that paddy rice must be planted in cropland. Classification accuracy was validated based on ground-data from 25 field survey quadrats measuring 600 m × 600 m. The results show that: multi-season paddy rice planting areas effectively was extracted by the method and adjusted early rice area of 1630.84 km², adjusted middle rice area of 556.21 km², and adjusted late rice area of 3138.37 km². The overall accuracy was 98.10%, with a kappa coefficient of 0.94.

18.
Reprod Fertil Dev ; 30(4): 634-642, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-28945985

RESUMO

In the present study, 21 forkhead box (Fox) genes were identified in Andrias davidianus, including 13 full-length genes and eight partial sequences. Phylogenetic analysis showed that most were conserved in other investigated amphibians, whereas the Foxk1 gene was found exclusively in A. davidianus. Molecular evolution analysis indicated that most Fox genes underwent purifying selection, whereas two sites of the adFoxp4 gene showed positive selection and were located on the adFoxp4 protein surface. Expression profiles of all Fox genes identified were analysed in the hypothalamic-pituitary-gonad axis by reverse transcription-quantitative polymerase chain reaction. Eighteen genes exhibited sexually dimorphic expression (15 ovary-biased and three testis-biased genes), whereas two genes showed no difference between ovary and testis. Further investigation of 12 selected sexually dimorphic Fox genes showed changes in the expression profile of 11 genes in the ovary of larvae reared at high temperatures (28°C). The results of the present study provide information on Fox genes in an amphibian and suggest that they play key roles in sexual development and reproduction in A. davidianus.


Assuntos
Fatores de Transcrição Forkhead/metabolismo , Expressão Gênica , Urodelos/metabolismo , Animais , Encéfalo/metabolismo , Evolução Molecular , Feminino , Fatores de Transcrição Forkhead/genética , Regulação da Expressão Gênica no Desenvolvimento , Masculino , Ovário/metabolismo , Hipófise/metabolismo , Conformação Proteica , Testículo/metabolismo , Urodelos/genética
19.
Nanoscale ; 9(36): 13610-13617, 2017 Sep 21.
Artigo em Inglês | MEDLINE | ID: mdl-28876001

RESUMO

Oil/water separation is of great importance for the treatment of oily wastewater, including immiscible light/heavy oil-water mixtures, oil-in-water or water-in-oil emulsions. Smart surfaces with responsive wettability have received extensive attention especially for controllable oil/water separation. However, traditional smart membranes with a switchable wettability between superhydrophobicity and superhydrophilicity are limited to certain responsive materials and continuous external stimuli, such as pH, electrical field or light irradiation. Herein, a candle soot coated mesh (CSM) with a larger pore size and a candle soot coated PVDF membrane (CSP) with a smaller pore size with underwater superoleophobicity and underoil superhydrophobicity were successfully fabricated, which can be used for on-demand immiscible oil/water mixtures and surfactants-stabilized oil/water emulsion separation, respectively. Without any continuous external stimulus, the wettability of our membranes could be reversibly switched between underwater superoleophobicity and underoil superhydrophobicity simply by drying and washing alternately, thus achieving effective and switchable oil/water separation with excellent separation efficiency. We believe that such smart materials will be promising candidates for use in the removal of oil pollutants in the future.

20.
Sci Rep ; 7(1): 9507, 2017 08 25.
Artigo em Inglês | MEDLINE | ID: mdl-28842650

RESUMO

Giardia is a worldwide spread protozoan parasite colonizing in small intestines of vertebrates, causing Giardiasis. The controversy about whether it is an extremely primitive eukaryote or just a highly evolved parasite has become a fetter to its uses as a model for both evolutionary and parasitological studies for years. Glycerophospholipid (GPL) synthesis is a conserved essential cellular process, and thus may retain some original features reflecting its evolutionary position, and this process should also have undergone parasitic adaptation to suit Giardia's dietary lipid-rich environment. Thus, GPL synthesis pathways may be a perfect object to examine the controversy over Giardia. Here, we first clarified Giardia's previously confusing GPL synthesis by re-identifying a reliable set of GPL synthesis genes/enzymes. Then using phylogenetic and comparative genomic analyses, we revealed that these pathways turn out to be evolutionarily primitive ones, but with many secondary parasitic adaptation 'patches' including gene loss, rapid evolution, product relocation, and horizontal gene transfer. Therefore, modern Giardia should be a mosaic of 'primary primitivity' and 'secondary parasitic adaptability', and to make a distinction between the two categories of features would restart the studies of eukaryotic evolution and parasitic adaptation using Giardia as a model system.


Assuntos
Vias Biossintéticas , Giardia/metabolismo , Giardíase/parasitologia , Glicerofosfolipídeos/metabolismo , Antiprotozoários/farmacologia , Evolução Biológica , Descoberta de Drogas , Regulação da Expressão Gênica , Regulação Enzimológica da Expressão Gênica , Giardia/classificação , Giardia/efeitos dos fármacos , Giardia/genética , Filogenia , Proteínas de Protozoários/metabolismo
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA