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1.
Food Chem ; 366: 130604, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34298395

RESUMO

The aroma profiles of fresh flat peach juice (FPJ) samples obtained from four different cultivars (RP1), (ZLP), (RP18), and (ZP) were characterized by gas chromatography-Mass spectrometry-olfactometry (GC-MS-O). Totally, 32 aroma-active compounds in FPJs were identified by GC-MS-O and further quantified. Of these, 14 aroma-active compounds presented odor activity values (OAVs) greater than 1, with several lactones and aldehydes contributing as key aroma-active components of FPJs. Partial least-squares regression (PLSR) revealed that RP18 was greatly related to "fruity", "sweet" and "peach-like" attributes, while ZLP was highly correlated with "floral" and "green and grassy" attributes, confirming the quantitative describe analysis (QDA) results. In addition, an aroma recombination experiment was conducted to mimic the aroma profile of flat peach juice based on the actual concentrations of RP18. Omission experiments indicated that lactones were very highly significant for the characteristic aroma of FPJ.


Assuntos
Prunus persica , Compostos Orgânicos Voláteis , Cromatografia Gasosa-Espectrometria de Massas , Odorantes/análise , Olfatometria , Compostos Orgânicos Voláteis/análise
2.
Food Chem ; 366: 130691, 2022 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-34339922

RESUMO

Millet Huangjiu (MHJ), a type of northern Huangjiu with a long history, has attracted considerable attention in China and East Asia for its unique flavor. To elucidate the changing course of aroma components during the fermentation process of MHJ, 15 MHJ samples prepared at different fermentation stages were assessed by gas chromatography-olfactometry (GC-O) and odor activity value (OAV) coupled with multivariate data analysis. A total of 66 volatile compounds were identified, among which 21 odorant active substances were regarded as the main aroma components (OAVs ≥ 1). The numbers and contents of esters in MHJs accumulated with the extension of fermentation time. Ethanol, ethyl acetate, phenylethyl alcohol and other 7 aromatic substances are the key flavoring agents in the final fermented MHJ. Orthogonal partial least squares-discriminant analysis (OPLS-DA) showed that 14 volatile compounds (formic acid, ethanol, etc.) are supposed to be the key substances that cause significant differences in MHJ flavor at different fermentation stages.


Assuntos
Odorantes , Compostos Orgânicos Voláteis , Fermentação , Cromatografia Gasosa-Espectrometria de Massas , Milhetes , Odorantes/análise , Compostos Orgânicos Voláteis/análise
3.
Food Chem ; 371: 131111, 2022 Mar 01.
Artigo em Inglês | MEDLINE | ID: mdl-34543928

RESUMO

Thyme (Thymus vulgaris L.) is widely used as a traditional spice in the cooking of goat meat (mutton) due to its distinctive flavor and the ability to weaken the "goaty flavor". To investigate the aroma characteristics of stewed mutton with thyme (SMT), four SMT samples prepared using different cooking utensils were analyzed by gas chromatography-mass spectrometry/olfactometry (GC-MS/O). Totally, 26 aroma-active compounds (AACs) were determined by GC-MS/O and further quantified. Among these, 20 AACs exhibited odor activity values (OAV) greater than 1. However, no significant differences existed among the four SMTs, which indicated that different utensils had little effect on the aroma profile of SMTs. Aroma recombination and omission experiments results showed that nonanal, (E)-2-octenal, and (E,E)-2,4-decadienal had the greatest contribution to the aroma profile of SMTs. These three compounds, together with dimethyl trisulfide, 3-methyl-butanal, octanal, (E)-2-decenal, (E)-2-nonenal, methanethiol, hexanal, (E)-2-undecenal, and 1-octen-3-ol, were confirmed as the key aroma compounds in SMTs.


Assuntos
Thymus (Planta) , Compostos Orgânicos Voláteis , Animais , Cabras , Carne , Odorantes , Olfatometria
4.
J Food Biochem ; : e13777, 2021 May 28.
Artigo em Inglês | MEDLINE | ID: mdl-34050545

RESUMO

Plant sprout foods exhibit a lot of biological activities including anti-inflammatory, antioxidative, anticancer, antidiabetes, anti-infection, and antiviral activities. Up to the present moment, plant sprout foods have received much attention due to their abundance, good bioavailability, and health benefits for human. This review highlights the biological activities of different plant sprout foods (viz., broccoli sprout, buckwheat sprout, wheat sprout, mung bean sprout, soybean sprout, and adkuzi bean sprout) using in vitro model, animal model, and human model. Furthermore, the bioavailability of plant sprout foods is also discussed. PRACTICAL APPLICATIONS: A review of the literature was conducted to biological activities of plant sprout foods, in addition to a summary of health benefits and bioavailability of sprout foods. Several biological activities of plant sprout foods with in vitro and in vivo evidence are currently unexplored in clinical trials, because the effects of sprout foods on human tissues and cells measured by tube test do not recapitulate the actual in vivo effects. Moreover, the safety of chemoprevention strategies using sprout foods that to protect against environmental exposures and other oxidative stress-related pathologies is important. Further research is warranted to evaluate bioavailability of individual forms.

5.
Am J Cancer Res ; 11(4): 1672-1682, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33948381

RESUMO

Prodrug-activating suicide gene therapy (PA suicide gene therapy for short) for cancer is to introduce cancer cells with suicide genes. The enzyme encoded by suicide gene is not toxic but is able to kill cancer cells by converting a non-toxic prodrug into a toxic compound. This approach is a promising cancer gene therapy that could reduce non-specific toxicity to normal tissue. However, there is no quantitative method to evaluate efficacy of suicide gene therapy in preclinical study. The aim of this study is to develop a new method to quantitatively evaluate and compare prodrug-activating suicide gene therapies. This study was carried out on an oral squamous cell carcinoma (OSCC) cell line CAL-27. Suicide genes were integrated into ROSA26 locus of CAL-27 by CRISPR-Cas9. CAL-27 cell lines stably expressing herpes simplex virus-thymidine kinase (TK) or yeast cytosine deaminase (CD) were used to evaluate and compare PA suicide gene therapies. The efficacies of PA suicide gene therapies were quantitatively evaluated from three aspects: effective prodrug concentration, prodrug treatment time, and bystander effect. This method also could be used for different types of suicide gene therapies and different types of cancer. When the prodrug concentration, treatment time, and rate of suicide gene-positive cells (related to bystander effect) are fixed, anti-cancer effects could be quantitatively measured. This information is important for suicide gene therapy preclinical development.

6.
Food Chem ; 357: 129786, 2021 Apr 20.
Artigo em Inglês | MEDLINE | ID: mdl-33984740

RESUMO

Fried mountain pepper (Litsea cubeba (Lour.) Pers.) oil is widely used as a traditional spice flavoring oil in Chinese home cooking. To investigate the effects of different frying temperatures on the aroma of fried mountain pepper oil (FPO), four FPO samples were analyzed by descriptive sensory analysis (DSA), E-nose, gas chromatography-olfactometry/detection frequency analysis (GC-O/DFA) and odor activity value (OAV) calculation. DSA and E-nose results both indicated that significant differences existed among 4 FPOs, among which FPO3 showed superiority in several sensory attributes. 16 and 20 aroma-active compounds were screened by DFA and OAV, respectively. Thereafter, three aroma recombination models were performed, and results indicated the model solution derived from the combination of OAV and DFA was more closely resembled the FPO aroma. Omission tests corroborated the significant contributions of 11 compounds (1-octen-3-ol, linalool, geraniol, nonanal, (E)-2-octenal, citral, citronellal, limonene, α-pinene, ß-myrcene and methylheptenone) to the characteristic aroma of FPO.

7.
Int J Biol Macromol ; 182: 115-128, 2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-33836188

RESUMO

With people's increasing awareness of diseases treatment, the researchers began to focus on drug delivery to the exact site of action at the optimal rate. Some researchers have proved that many nanostructures loaded with drugs are significantly better than conventional nanostructures. However, the materials from which the nanostructure determines its performance. To use it as a pharmaceutical ingredient, it must meet strict safety regulatory standards worldwide. Therefore, people's attention has paid to easily available natural substances. As far as we know, bioactive polysaccharides are excellent candidates for realizing these purposes. To be precise, due to the natural availability of polysaccharides, it has been widely used in the research of Nano-biocarriers loaded with drugs. Based on the above analysis, the nanomaterials developed through the laboratory have great potential for upgrading to market products. Therefore, it is of great significance to review the latest progress of polysaccharide-based Nano-biocarriers in drug delivery and their application in diseases treatment. In this work, we focused on the preparation of polysaccharides-based Nano-biocarriers, commonly used polysaccharides for preparing Nano-biocarriers, and drugs loaded on polysaccharides-based Nano-biocarriers to treat diseases. Shortly, polysaccharide-based Nano-biocarriers will be increasingly used in drug delivery and treatment of diseases.


Assuntos
Nanocápsulas/química , Polissacarídeos/química , Animais , Anti-Inflamatórios/administração & dosagem , Antineoplásicos/administração & dosagem , Humanos , Nanocápsulas/classificação
8.
Food Res Int ; 139: 109841, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33509465

RESUMO

The commercial kiwifruit juice is deficient in a theoretical basis for the control of aroma characteristics during sterilization. To investigate the different sterilization methods on the aroma of kiwifruit juice, three sterilized kiwifruit juice samples, including pasteurization (PS), high temperature short time (HTST) and high hydrostatic pressure (HHP) sterilization, were observed. Results showed that a total of 15 major aroma-active compounds were identified in fresh kiwifruit juice by combination of detection frequency (DF) analysis and odor activity value (OAV); while the changes of these aroma-active compounds during PS, HTST and HHP sterilization were further studied. Quantitative descriptive analysis (QDA) was applied to validate the sensory differences, showing fruity and grassy notes changed a lot after sterilization, and the HHP sample was similar to fresh sample (FS) in comparison of samples treated by other sterilization methods. Further partial least squares regression analysis (PLSR) coincided with the overall note. Among these aroma-active compounds, the decrease of C6 aldehydes and C6 alcohols such as hexanal, (E)-2-hexenal and 1-hexanol might result in the great change of grassy note while the change of fruity note might be well correlated with the decrease of esters such as methyl butyrate and ethyl butyrate during processing.


Assuntos
Odorantes , Compostos Orgânicos Voláteis , Frutas/química , Cromatografia Gasosa-Espectrometria de Massas , Pressão Hidrostática , Odorantes/análise , Compostos Orgânicos Voláteis/análise
9.
Food Chem ; 345: 128748, 2021 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-33340890

RESUMO

Flavor is a key attribute of fried oil that shows a critical correlation with temperature. Therefore, selecting the appropriate temperature is important in preparing fried shallot oil (FSO). Volatile compounds from five different FSOs were identified and comparatively studied using gas chromatography-mass spectrometry (GC-MS) coupled with multivariate data analysis, including principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). GC-MS results identified a total of 93 volatiles, among which aldehydes, alcohols, pyrazines, and sulfur-containing compounds were the major compounds. Eighteen compounds had odor active values (OAV) >1. Among the compounds, hexanal, (E)-2-heptenal, (E)-2-octenal, dipropyl disulfide, 2-ethyl-3,5-dimethylpyrazine, and 1-octen-3-ol were important to the overall aroma profile of FSOs. In the PCA model, all the detected FSOs were divided into three clusters, which were assigned as cluster A (FSO5), B (FSO4), and C (the rest FSOs). Multivariate data analyses revealed that nonanal, 2-ethyl-5-methylpyrazine, (E,E)-2,4-decadienal, (E)-2-heptenal, and hexanal contributed positively to the classification of different FSOs. GC-MS coupled with multivariate data analysis could be used as a convenient and efficient analytical method to classify raw materials.


Assuntos
Culinária , Análise de Alimentos/métodos , Cromatografia Gasosa-Espectrometria de Massas , Óleos Vegetais/química , Cebolinha Branca/química , Temperatura , Compostos Orgânicos Voláteis/análise , Análise Multivariada , Odorantes/análise , Paladar
10.
Food Chem ; 377: 131984, 2021 Dec 31.
Artigo em Inglês | MEDLINE | ID: mdl-34995962

RESUMO

Fried huajiao oil (FHO) samples prepared with red or green huajiao are widely applied in different Chinese cuisines due to their own aroma characteristics. To investigate their different aroma profiles, 2 red and 3 green FHOs were analyzed by quantitative descriptive sensory analysis (QDA) and gas chromatography-olfactometry/aroma intensity (GC-O/AI). QDA results showed a distinct difference among FHOs in terms of all sensory attributes. Thirty odorants with high OAVs and AIs were screened from 5 FHOs, among which ß-myrcene, (E)-2-heptenal, limonene, α-terpineol and p-cymene were the major characteristic compounds of FHOs. In addition, through orthogonal partial least square discriminate analysis (OPLS-DA), linalool, linalyl acetate, and 1,8-cineole were considered as the volatile markers for classification of FHOs with red and green huajiao. Thereafter, aroma recombination and omission tests were performed to characterize the key aroma compounds of red and green FHOs.

11.
Nucleic Acids Res ; 48(19): 11146-11161, 2020 11 04.
Artigo em Inglês | MEDLINE | ID: mdl-32986843

RESUMO

The six-subunit origin recognition complex (ORC), a DNA replication initiator, defines the localization of the origins of replication in eukaryotes. The Orc6 subunit is the smallest and the least conserved among ORC subunits. It is required for DNA replication and essential for viability in all species. Orc6 in metazoans carries a structural homology with transcription factor TFIIB and can bind DNA on its own. Here, we report a solution structure of the full-length human Orc6 (HsOrc6) alone and in a complex with DNA. We further showed that human Orc6 is composed of three independent domains: N-terminal, middle and C-terminal (HsOrc6-N, HsOrc6-M and HsOrc6-C). We also identified a distinct DNA-binding domain of human Orc6, named as HsOrc6-DBD. The detailed analysis of the structure revealed novel amino acid clusters important for the interaction with DNA. Alterations of these amino acids abolish DNA-binding ability of Orc6 and result in reduced levels of DNA replication. We propose that Orc6 is a DNA-binding subunit of human/metazoan ORC and may play roles in targeting, positioning and assembling the functional ORC at the origins.


Assuntos
Replicação do DNA , DNA/metabolismo , Complexo de Reconhecimento de Origem/metabolismo , Origem de Replicação , Humanos , Ligação Proteica , Domínios Proteicos
12.
Food Sci Nutr ; 8(4): 2088-2093, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32328275

RESUMO

Self-made milk knots in Xinjiang Kazakh ethnic group were used as material to establish the quality assessment system of flavor quality. The fuzzy analytic hierarchy process based on the optimal consistency matrix was used to evaluate the quality of the samples qualitatively and quantitatively. Its result is consistent with the cluster analysis of the SOM neural network. The results showed that the milk knot samples of Altay had differences with the milk knot samples of Yili. The comprehensive evaluation system is feasible and can evaluate the quality of milk knot samples by flavor characteristics. This can provide a reference for further research on the origin of differences between two types of milk knot samples.

13.
Int J Anal Chem ; 2019: 8963191, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31057622

RESUMO

A single-factor gradual optimization method was developed in this experiment in order to improve the headspace solid-phase microextraction (HS-SPME) effect of volatile compounds in pepper chicken soup. The different extraction conditions included fibers with different coating materials, sample volume, extraction temperature, and extraction time. The total peak areas and the numbers of valid peaks were compared and analyzed as the indicators of condition optimization. Gas chromatography-mass spectrometry (GC-MS) results showed that the four factors all have significant impact on the extraction effect of volatiles in pepper chicken soup. Using the principal component analysis (PCA), the optimal conditions of HS-SPME were inferred below: an extraction fiber of 50/30µm DVB/CAR/PDMS, a sample volume of 7 g, an extraction temperature of 65°C, and an extraction time of 30 min. Compared to the original extraction conditions, the optimized conditions were especially advantageous for the comprehensive analysis of volatiles, which could be potentially used in further study of soup.

14.
Pak J Pharm Sci ; 32(6): 2625-2632, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31969295

RESUMO

Ferula lehmannii Boiss (FLB) is a perennial plant that belongs to the family Apiaceae, which is a traditional remedy used to treat gastric ulcers in Xinjiang. The main purpose of the research is to investigate the possible antiulcer effect of three different extracts, water decoction (WD), fresh liquid (FL), and chloroform extract (CE), using a model of acetic acid-induced gastric ulcer. 56 rats were divided into seven groups (n=8) and treated ranitidine and extracts of FLB. After 12 days of treatment, the ulcer index and biochemical parameters were evaluated. In all tested groups, the results indicated that the chloroform extract and water decoction highly significantly decreased the mucosal damage index as compared to the model group, restoration of glutathione per oxidase (GSH-PX) levels and super oxide dismutase (SOD) activity, and reduction of malondialdehyde (MDA) levels. The ulcer inhibition rate of water decoction group, fresh liquid and chloroform extract group reached 25.30%, 4.96% and 30.87%, respectively. The macroscopic observations were supported by histological findings. 44, 31, 32 compounds were identified through GC-MS analysis of different extracts. In conclusion, FLB exhibits potential anti-ulcer activity attributed to its high content terpenoid, phytosterin and fatty acid, the underlying antiulcer mechanism might be relevant to the reduction in inflammation and oxidative stress.


Assuntos
Antioxidantes/uso terapêutico , Ferula/química , Extratos Vegetais/uso terapêutico , Folhas de Planta/química , Úlcera Gástrica/tratamento farmacológico , Animais , Antioxidantes/isolamento & purificação , Mucosa Gástrica/efeitos dos fármacos , Mucosa Gástrica/patologia , Glutationa Peroxidase/metabolismo , Malondialdeído , Fitoterapia/métodos , Extratos Vegetais/isolamento & purificação , Ratos , Ratos Sprague-Dawley , Úlcera Gástrica/patologia , Superóxido Dismutase/metabolismo
15.
Cell Rep ; 23(6): 1691-1705, 2018 05 08.
Artigo em Inglês | MEDLINE | ID: mdl-29742426

RESUMO

Normal brain functions depend on the balanced development of excitatory and inhibitory synapses. Our knowledge of the molecular mechanisms underlying inhibitory synapse formation is limited. Neuroligin-2 (NL2), a transmembrane protein at inhibitory postsynaptic sites, is capable of initiating inhibitory synapse formation. In an effort to search for NL2 binding proteins and the downstream mechanisms responsible for inhibitory synapse development, we identify LHFPL4/GARLH4 as a major NL2 binding partner that is specifically enriched at inhibitory postsynaptic sites. LHFPL4/GARLH4 and NL2 regulate the protein levels and synaptic clustering of each other in the cerebellum. Lhfpl4/Garlh4-/- mice display profound impairment of inhibitory synapse formation as well as prominent motor behavioral deficits and premature death. Our findings highlight the essential role of LHFPL4/GARLH4 in brain functions by regulating inhibitory synapse formation as a major NL2 binding partner.


Assuntos
Comportamento Animal , Moléculas de Adesão Celular Neuronais/metabolismo , Proteínas de Membrana/genética , Atividade Motora , Proteínas do Tecido Nervoso/metabolismo , Inibição Neural , Neurogênese , Sinapses/metabolismo , Animais , Células Cultivadas , Cerebelo/metabolismo , Hipocampo/citologia , Camundongos Endogâmicos C57BL , Camundongos Knockout , Proteínas do Tecido Nervoso/genética , Neurônios/metabolismo , Ligação Proteica
16.
Nat Prod Res ; 32(7): 863-866, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-28836454

RESUMO

Chinese purple yam is famous for therapeutic and nutritional values in lowering blood glucose, blood pressure and even preventing and treating cardiovascular diseases. However, traditional extraction techniques for the functional polyphenolic compounds mostly utilise unfriendly organic solvent and easily cause degradation of polyphenols. In this study, a novel ionic liquid-based ultrasound-assisted extraction (IL-UAE) technique was utilised to obtain polyphenols. The optimal extraction condition included: solid-liquid ratio (0.05 g/mL), ionic liquid concentration (1.05 M), extraction temperature (67°C) and ultrasonic time (37 min). The maximum free radical scavenging activity (86.21%) and total antioxidant activity (2.21 mM) were achieved and matched well with the predicted values, superior to that of the extract from regular ethanol-based UAE. IL-UAE could be a rapid and green technique for efficient extraction of polyphenols from purple yam with low solvent consumption and few structural deterioration, exhibiting application potential in food and pharmaceutical industries.


Assuntos
Fracionamento Químico/métodos , Dioscorea/química , Líquidos Iônicos/química , Polifenóis/isolamento & purificação , Extratos Vegetais/química , Polifenóis/química , Solventes , Temperatura , Ultrassom
17.
Lipids ; 51(8): 931-9, 2016 08.
Artigo em Inglês | MEDLINE | ID: mdl-27262314

RESUMO

The effects of dietary apricot kernel oil (AKO), which contains high levels of oleic and linoleic acids and lower levels of α-tocopherol, were evaluated in a rat model of cyclophosphamide-induced immunosuppression. Rats had intraperitoneal injection with cyclophosphamide to induce immunosuppression and were then infused with AKO or normal saline (NS) for 4 weeks. Enzyme-linked immunosorbent assays were used to detect antimicrobial factors in lymphocytes and anti-inflammatory factors in hepatocytes. Hematoxylin & eosin staining was conducted prior to histopathological analysis of the spleen, liver, and thymus. Significant differences were observed between the immune functions of the healthy control group, the normal saline group, and the AKO group. Compared to the normal saline-treated group, lymphocytes isolated from rats administered AKO showed significant improvement in immunoglobulin (Ig)A, IgM, IgG, interleukin (IL)-2, IL-12, and tumor necrosis factor-α (TNF-α) levels (p < 0.01). Liver tissue levels of malondialdehyde and activities of superoxide dismutase and glutathione peroxidase indicated reduced oxidative stress in rats treated with AKO (p < 0.01). Dietary AKO positively affected rat growth and inhibited cyclophosphamide-associated organ degeneration. These results suggested that AKO may enhance the immune system in vivo. These effects may reflect the activities of intermediate oleic and linoleic acid metabolites, which play a vital role in the immune system, and the α-tocopherol in AKO may further enhance this phenomenon. Thus, the use of AKO as a nutritional supplement can be proposed to ameliorate chemotherapy-associated immunosuppression.


Assuntos
Ciclofosfamida/efeitos adversos , Hospedeiro Imunocomprometido/efeitos dos fármacos , Linfócitos/imunologia , Óleos Vegetais/administração & dosagem , Prunus armeniaca/química , Animais , Ciclofosfamida/administração & dosagem , Modelos Animais de Doenças , Hepatócitos/efeitos dos fármacos , Hepatócitos/metabolismo , Imunoglobulinas/metabolismo , Infusões Parenterais , Injeções Intraperitoneais , Interleucina-12/metabolismo , Interleucina-2/metabolismo , Linfócitos/efeitos dos fármacos , Masculino , Estresse Oxidativo/efeitos dos fármacos , Óleos Vegetais/farmacologia , Ratos , Fator de Necrose Tumoral alfa/metabolismo
18.
Artigo em Inglês | MEDLINE | ID: mdl-23416288

RESUMO

Changes in the aroma characteristics of mutton process flavors (MPFs) prepared from sheep bone protein hydrolysates (SBPHs) with different degrees of hydrolysis (DH) were evaluated using gas chromatography-mass spectrometry (GC-MS), gas chromatography-olfactometry (GC-O), and descriptive sensory analysis (DSA). Five attributes (muttony, meaty, roasted, mouthful, and simulate) were selected to assess MPFs. The results of DSA showed a distinct difference among the control sample MPF0 and other MPF samples with added SBPHs for different DHs of almost all sensory attributes. MPF5 (DH 25.92%) was the strongest in the muttony, meaty, and roasted attributes, whereas MPF6 (DH 30.89%) was the strongest in the simulate and roasted attributes. Thirty-six compounds were identified as odor-active compounds for the evaluation of the sensory characteristics of MPFs via GC-MS-O analysis. The results of correlation analysis among odor-active compounds, molecular weight, and DSA further confirmed that the SBPH with a DH range of 25.92-30.89% may be a desirable precursor for the sensory characteristics of MPF.


Assuntos
Osso e Ossos/química , Aromatizantes/análise , Carne/análise , Odorantes/análise , Proteínas/química , Carneiro Doméstico/metabolismo , Adulto , Animais , Cromatografia Gasosa , Feminino , Cromatografia Gasosa-Espectrometria de Massas/métodos , Humanos , Hidrólise , Análise dos Mínimos Quadrados , Masculino , Pessoa de Meia-Idade , Percepção Gustatória , Compostos Orgânicos Voláteis/análise
19.
Int J Cancer ; 131(6): 1445-54, 2012 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-22116711

RESUMO

Oblongifolin C (OC) was identified as a potent apoptosis inducer from an herbal plant, Garcinia yunnanensis, during our previous bioassay-guided drug screening. In this study, we investigated the signaling pathways through which OC activated apoptosis in HeLa cells. We also compared the IC(50) values of OC with that of etoposide, paclitaxel and vinblastine in multiple cancer cell lines including HER2 and P-glycoprotein overexpressing cells. In addition, the in vivo antitumor effect of OC was studied in nude mice model. Our results showed that OC induced a caspase-dependent apoptosis by triggering a series of events in HeLa cells including Bax translocation, cytochrome c release, caspase-3 activation, chromosome fragmentation followed by caspase-8 activation, Bid cleavage and eventually cell death. Addition of a pan-caspase inhibitor or overexpression of an anti-apoptotic protein, Bcl-xL, prevented OC-induced cell death. Moreover, OC exhibited a wide anticancer spectrum in multiple cancer cell lines with comparable IC(50) values, regardless of the expression levels of HER2 and P-glycoprotein. In contrast, the IC(50) values of three clinical anticancer drugs, etoposide, paclitaxel and vinblastine were significantly elevated in HER2 and/or P-glycoprotein overexpressing cells. Furthermore, OC showed a similar antitumor effect but lower general toxicity than etoposide against xenografted human tumors in nude mice model. All these data suggested that OC is a promising apoptosis inducer with the potential to be developed into a clinical anticancer drug.


Assuntos
Antineoplásicos Fitogênicos/farmacologia , Apoptose/efeitos dos fármacos , Garcinia/química , Terpenos/farmacologia , Proteína X Associada a bcl-2/metabolismo , Membro 1 da Subfamília B de Cassetes de Ligação de ATP/análise , Animais , Caspases/fisiologia , Feminino , Células HeLa , Humanos , Camundongos , Camundongos Endogâmicos BALB C , Mitocôndrias/fisiologia , Receptor ErbB-2/análise , Ensaios Antitumorais Modelo de Xenoenxerto
20.
Nat Prod Res ; 25(12): 1208-11, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21711172

RESUMO

Ansu apricots growing wild in north Xinjiang are recognised as being one of the major wild-plant resources in China. In order to improve the level of comprehensive utilisation and the number of cultivated apricot varieties, the chemical composition of ansu apricot oil was analysed by capillary GC-MS and elucidated based on the standard mass spectral data; the antioxidant activities were also evaluated. Seven components of ansu apricots oil were identified, and the total unsaturated fatty acid (FA) (TUFA) and total essential FA (TEFA) contents of the ansu apricot oil were found to be 90.35 g/100 g and 48.93 g/100 g, respectively. The scavenging capacity of the ansu apricots oil in the superoxide anion radical system and the hydroxyl radical system performed better than in the DPPH radical system. The IC50 values of the ansu apricot oil for the superoxide anion radical system and the hydroxyl radical system were 0.15 mg mL⁻¹ and 0.30 mg mL⁻¹, respectively: stronger than that of the control (ascorbic acid). In the DPPH system, the IC50 value of the ansu apricot oil was 0.50 mg mL⁻¹, and the IC50 value of ascorbic acid was 0.30 mg mL⁻¹, but within the selected dosage, the highest scavenging capacity of ansu apricot oil was higher than the control. The results obtained in this study clearly suggest that ansu apricot oil is a natural source of antioxidants and could serve as a functional food ingredient with potential application in food products and thus provide related health benefits.


Assuntos
Antioxidantes/farmacologia , Óleos Voláteis/química , Óleos Voláteis/farmacologia , Prunus/química , Ácido Ascórbico , China , Ácidos Graxos Essenciais/análise , Ácidos Graxos Insaturados/análise , Cromatografia Gasosa-Espectrometria de Massas , Radical Hidroxila/química , Concentração Inibidora 50 , Superóxidos/química
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