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1.
Sci Total Environ ; 776: 145950, 2021 Feb 18.
Artigo em Inglês | MEDLINE | ID: mdl-33647641

RESUMO

Environmental BaP (benzo(a)pyrene) and its metabolite BPDE (benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide) inhibit proliferation of human villous trophoblast cells, which might further induce recurrent miscarriage (RM). However, the underlying mechanisms remain largely unknown. In this work, we identified a novel lncRNA HZ01 (lnc-HZ01) that is up-regulated in both RM tissues and BPDE-exposed trophoblast cells. Lnc-HZ01 inhibits trophoblast cell proliferation and induces miscarriage. Mechanistically, lnc-HZ01 promotes MXD1 mRNA transcription by up-regulating its transcription factor c-JUN and also enhances MXD1 protein stability by up-regulating its deubiquitin enzyme USP36. Reversely, MXD1 up-regulates lnc-HZ01 level by enhancing its RNA stability due to the increased level of m6A RNA methylation on lnc-HZ01, the first example that m6A modification regulates trophoblast cell functions. Thus, lnc-HZ01 and MXD1 comprise a positive self-feedback loop, which is up-regulated in both RM tissues and BPDE-exposed trophoblast cells. Once this loop is activated by BaP or BPDE exposure, both pathways in this loop would be up-regulated, promote EIF4E transcription, inhibit trophoblast cell proliferation, and further induce miscarriage. This work provides new clinical and scientific understanding in unexplained miscarriage.

2.
Medicine (Baltimore) ; 100(9): e25070, 2021 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-33655985

RESUMO

RATIONALE: Thyroid nodule rupture is a rare complication after microwave ablation (MWA). The ultrasonographic characteristics, clinical course, treatment, or prognosis of thyroid nodule rupture after ablation have not been systematically summarized. Three cases with thyroid nodule rupture after MWA were reported in this study, including the characteristic ultrasound images before ablation and after rupture. Meanwhile, we investigated the etiology, diagnosis, treatment and prevention of the rupture. These findings can provide references for the future clinical practice. PATIENTS CONCERNS: All 3 patients were pathologically diagnosed as benign thyroid nodules by core needle biopsy and then received 1 session of MWA. DIAGNOSES: Fourteen days to 1 month after MWA later, all 3 patients presented with abrupt neck pain and swelling, and 1 of them had a fever. Ultrasound examinations shared common features that the rupture of thyroid capsule and a soft-tissue mass with unclear margin in front of the thyroid gland, which connected with the post-ablation nodule. Three patients were diagnosed as thyroid nodule ruptures. INTERVENTIONS: All 3 patients received conservative management after the ruptures. With the treatment of intravenous antibiotics for 1 week, the neck swelling of patients 1 and 2 both disappeared. The aggravation of neck swelling was found in patient 3. Ultrasonography of the neck revealed irregular fluid echo in the soft-tissue mass, suggesting abscess formation. Aspiration and irrigation were performed. The neck swelling regressed gradually over another 2 weeks with the treatment of antibiotics. Two months after ablation, ultrasound examination showed that the mass had completely disappeared. OUTCOMES: None of the 3 patients underwent open surgery due to thyroid nodule rupture. At 1-year follow-up, the volume reduction rate of thyroid nodules in 3 patients were as follows: 100%, 98.1% and 90.7%. LESSONS: Nodule rupture is a rare but severe complication after MWA of the thyroid nodules. The diagnosis can be confirmed by clinical symptoms and ultrasound examination, and most nodule ruptures could be cured with conservative treatment. Grasping the characteristics of ultrasound imaging during the course of disease, and dynamically assessing course of disease progression by ultrasonography could avoid unnecessary imaging examinations or invasive procedures.


Assuntos
Micro-Ondas/uso terapêutico , Terapia por Radiofrequência/efeitos adversos , Glândula Tireoide/diagnóstico por imagem , Nódulo da Glândula Tireoide/diagnóstico , Ultrassonografia/métodos , Adulto , Feminino , Humanos , Masculino , Micro-Ondas/efeitos adversos , Ruptura , Nódulo da Glândula Tireoide/terapia
3.
Adv Exp Med Biol ; 1300: 3-19, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33523427

RESUMO

In this Chapter, we systematically and comprehensively described various environmental harmful factors. They were classified into four aspects: physical factors, chemical factors, biological factors, and physiological and psychological stress factors. Their classification, modes of presence, toxicity and carcinogenicity, routes of exposure to human and toxic effects on the female reproductive health were introduced. It is expected that the exposure routes could be controlled and eliminated, and the pathogenic mechanism of environmental harmful factors should be investigated and explained to protect female reproductive health.


Assuntos
Exposição Ambiental , Saúde Reprodutiva , Exposição Ambiental/efeitos adversos , Feminino , Humanos , Reprodução , Saúde da Mulher
4.
Cancer Sci ; 2021 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-33565687

RESUMO

Reliable and noninvasive biomarkers for the early diagnosis of non-small cell lung cancer (NSCLC) are an unmet need. This study aimed to screen and validate potential urinary biomarkers for the early diagnosis of NSCLC. By protein mass spectrometry, urinary MDH2 was found abundant both in lung cancer patients and lung cancer mice compared with controls. Urine samples obtained as retrospective and prospective cohort including 1091 NSCLC patients and 736 healthy controls were measured by ELISA. Compared to healthy controls, patients with stage I NSCLC had higher urinary MDH2. The area under the receiver operating characteristic curve (AUC) for the urinary MDH2 was 0.7679 and 0.7234 in retrospective and prospective cohort for distinguishing stage I cases from controls. Urinary MDH2 level was correlated with gender and smoking history. MDH2 expression was elevated in lung cancer tissues. MDH2 knockdown via shRNA inhibited proliferation of lung cancer cells. Our study demonstrates that the urinary MDH2 concentration is higher in early-stage NSCLC patients than that in controls and may serve as a potential biomarker for the early detection of NSCLC.

5.
Cell Biol Toxicol ; 2021 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-33566220

RESUMO

Normal pregnancy is essential for human reproduction. However, environmental BaP (benzo(a)pyrene) and its metabolite BPDE (benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide) induce dysfunctions of human trophoblastic cells, which could further result in miscarriage. Yet, the molecular mechanisms remain poorly understood. In this work, a novel lnc-HZ03 and a novel miR-hz03 were identified. Both lnc-HZ03 and miR-hz03 were highly expressed in human recurrent miscarriage villous tissues and in BPDE-exposed trophoblastic cells. Lnc-HZ03 and miR-hz03 upregulated each other, forming a positive feedback loop. MiR-hz03 could also upregulate p53 level by enhancing its mRNA stability. Both lnc-HZ03 and p53 mRNA contained the target site for miR-hz03 and could directly interact with miR-hz03. It was this target site instead of its mutant on lnc-HZ03 that regulated p53 expression. Subsequently, the upregulated p53 facilitated SAT1 transcription and enhanced SAT1-catalyzed spermine metabolism, which further resulted in trophoblastic cell apoptosis and induced miscarriage. All together, the p53/SAT1 pathway upregulated by lnc-HZ03 and miR-hz03 could promote BPDE-induced human trophoblastic cell apoptosis and the occurrence of miscarriage, shedding novel light on the causes of miscarriage. Graphical abstract Lnc-HZ03 and miR-hz03 regulate the occurrence of recurrent miscarriage (RM). In human trophoblastic cells, lnc-HZ03 upregulates miR-hz03 level. MiR-hz03 increases the RNA stability of lnc-HZ03 and p53 mRNA. P53 promotes SAT1 transcription and reduces its cellular spermine content, resulting in cell apoptosis. Under normal conditions, lnc-HZ03/miR-hz03 and p53/SAT1 pathways are downregulated, maintaining normal pregnancy. After exposure to BPDE, lnc-HZ03/miR-hz03 and p53/SAT1 pathways are upregulated and finally induce miscarriage.

6.
Food Res Int ; 139: 109841, 2021 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-33509465

RESUMO

The commercial kiwifruit juice is deficient in a theoretical basis for the control of aroma characteristics during sterilization. To investigate the different sterilization methods on the aroma of kiwifruit juice, three sterilized kiwifruit juice samples, including pasteurization (PS), high temperature short time (HTST) and high hydrostatic pressure (HHP) sterilization, were observed. Results showed that a total of 15 major aroma-active compounds were identified in fresh kiwifruit juice by combination of detection frequency (DF) analysis and odor activity value (OAV); while the changes of these aroma-active compounds during PS, HTST and HHP sterilization were further studied. Quantitative descriptive analysis (QDA) was applied to validate the sensory differences, showing fruity and grassy notes changed a lot after sterilization, and the HHP sample was similar to fresh sample (FS) in comparison of samples treated by other sterilization methods. Further partial least squares regression analysis (PLSR) coincided with the overall note. Among these aroma-active compounds, the decrease of C6 aldehydes and C6 alcohols such as hexanal, (E)-2-hexenal and 1-hexanol might result in the great change of grassy note while the change of fruity note might be well correlated with the decrease of esters such as methyl butyrate and ethyl butyrate during processing.

7.
Artigo em Inglês | MEDLINE | ID: mdl-33480834

RESUMO

A Gram-strain-negative, facultatively anaerobic, motile, rod-shaped and flagellated marine bacterium, designated SM6T, was isolated from surface seawater collected in Daya Bay (Guangdong, China). Phylogenetic analysis based on 16S rRNA gene sequences, multilocus sequence analysis, phylogenomic analysis of single-copy gene families and whole genome data showed that strain SM6T belonged to the genus Vibrio. The closest phylogenetic relatives of SM6T were Vibrio plantisponsor MSSRF60T (97.38 % 16S rRNA gene sequence pairwise similarity), Vibrio variabilis R-40492T (97.27 %), Vibrio aestuarianus ATCC 35048T (97.21 %) and Vibrio sagamiensis LC2-047T (97.3 %). Growth of strain SM6T occurred at 10-45 °C (optimum 30 °C), at pH 6.0-9.0 (optimum 6.0) and in the presence of 0-10 % (w/v) NaCl (optimum 3-8 %). The predominant fatty acids (>10 %) were summed feature 3 (C16 : 1 ω7c or/and C16 : 1 ω6c), C16 : 0 and summed feature 8 (C18 : 1 ω7c or/and C18 : 1 ω6c). The DNA G+C content of the assembled genomic sequences was 47.37 % for strain SM6T. Average nucleotide identity values between SM6T and its reference species were lower than the threshold for species delineation (95-96 %); in silico DNA-DNA hybridization further showed that the strains shared less than 70 % similarity. On the basis of evidence from the present polyphasic study, strain SM6T is considered to represent a novel species of the genus Vibrio, for which the name Vibrio agarilyticus sp. nov. is proposed. The type strain is SM6T (=KCTC 82076T=MCCC 1K04327 T).


Assuntos
Filogenia , Água do Mar/microbiologia , Vibrio/classificação , Ágar/metabolismo , Técnicas de Tipagem Bacteriana , Composição de Bases , Baías , China , DNA Bacteriano/genética , Ácidos Graxos/química , Tipagem de Sequências Multilocus , Hibridização de Ácido Nucleico , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Vibrio/isolamento & purificação
8.
Technol Cancer Res Treat ; 20: 1533033820988525, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-33506742

RESUMO

BACKGROUND: Long noncoding RNAs have been demonstrated to play important roles in different kinds of human malignancy. The purpose of this study was to evaluate the diagnostic and prognostic value of long intergenic non-protein coding RNA 1232 (LINC01232) in patients with pancreatic adenocarcinoma (PAAD) and further explore the clinical significance of the potential miRNAs that might be sponged by LINC01232. METHODS: The potential target miRNAs that might be sponged by LINC01232 were analyzed using bioinformatics analysis. The Real-Time quantitative PCR was adopted to measure the relative expression of LINC01232 and target miRNAs in PAAD serum and tissue samples. The diagnostic and prognostic value of LINC01232 was evaluated using the receiver operating characteristic analysis and Kaplan-Meier survival analysis, respectively. RESULTS: LINC01232 expression was upregulated in PAAD serum and tissues and associated with patients' TNM stage. Serum LINC01232 expression had diagnostic value, and the high levels of LINC01232 could predict unfavorable prognosis in PAAD patients. miR-204-5p, miR-370-5p and miR-654-3p were proposed as 3 targets of LINC01232 in PAAD, and their decreased expression levels in PAAD patients showed certain clinical significance in diagnosis and prognosis. CONCLUSION: The data of this study revealed that LINC01232 expression is upregulated in PAAD serum and tissue samples with considerable diagnostic and prognostic significance. In addition, miR-204-5p, miR-370-5p and miR-654-3p may be sponged by LINC01232 in PAAD, which also show potencies in PAAD diagnosis and prognosis.

9.
BMC Microbiol ; 21(1): 22, 2021 Jan 11.
Artigo em Inglês | MEDLINE | ID: mdl-33430771

RESUMO

BACKGROUND: Human noroviruses (HuNoVs) are a major cause of nonbacterial gastroenteritis in all age groups worldwide. HuNoVs can be detected in vitro using molecular assays such as RT-PCR and RT-qPCR. However, these molecular-based techniques require special equipment, unique reagents, experienced personnel, and extended time to obtain results. Besides, the diversity of viral genotypes is high. Therefore, methods that are rapid, broad-range and effective in the detection of HuNoVs are desiderated for screening the feces or vomit of infected people during outbreaks. RESULTS: In this study, a colloidal-gold-based immunochromatographic assay (ICA) was developed for effective detection of HuNoVs in clinical samples. Monoclonal antibodies (MAbs) against the shell (S) domain in the major capsid protein of HuNoVs were used in the ICA. The limitations of detection for HuNoVs in clinical samples were 1.2 × 106 genomic copies per gram of stool sample (gc/g) and 4.4 × 105 gc/g for genogroup I and II (GI and GII) HuNoVs, respectively. A total of 122 clinical samples were tested for HuNoVs by ICA and compared against RT-qPCR. The relative sensitivity, specificity and agreement of ICA was 84.2% (95% CI: 83.6-84.8%), 100.0% (95% CI: 98.5-100.0%) and 87.7% (95% CI: 85.6-89.8%), respectively. No cross-reaction with other common enteric viruses or bacteria was observed. The ICA detected a broad range of genotypes, including GI.1, GI.3, GI.4, GI.6, GI.14, GII.2, GII.3, GII.4, GII.6, GII.13, and GII.17 HuNoVs. CONCLUSIONS: This study demonstrates that ICA targeting the S domain of VP1 is a promising candidate for effectively identifying the different genotypes of HuNoVs in clinical samples with high sensitivity and specificity.

10.
New Phytol ; 229(1): 230-244, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-32749703

RESUMO

Clarifying the coordination of leaf hydraulic traits with gas exchange across closely-related species adapted to varying rainfall can provide insights into plant habitat distribution and drought adaptation. The leaf hydraulic conductance (Kleaf ), stomatal conductance (gs ), net assimilation (A), vein embolism and abscisic acid (ABA) concentration during dehydration were quantified, as well as pressure-volume curve traits and vein anatomy in 10 Caragana species adapted to a range of mean annual precipitation (MAP) conditions and growing in a common garden. We found a positive correlation between Ψleaf at 50% loss of Kleaf (Kleaf P50 ) and maximum Kleaf (Kleaf-max ) across species. Species from low-MAP environments exhibited more negative Kleaf P50 and turgor loss point, and higher Kleaf-max and leaf-specific capacity at full turgor, along with higher vein density and midrib xylem per leaf area, and a higher ratio of Kleaf-max : maximum gs . Tighter stomatal control mediated by higher ABA accumulation during dehydration in these species resulted in an increase in hydraulic safety and intrinsic water use efficiency (WUEi ) during drought. Our results suggest that high hydraulic safety and efficiency combined with greater stomatal sensitivity triggered by ABA production and leading to greater WUEi provides drought tolerance in Caragana species adapted to low-MAP environments.

11.
Food Chem ; 345: 128748, 2021 May 30.
Artigo em Inglês | MEDLINE | ID: mdl-33340890

RESUMO

Flavor is a key attribute of fried oil that shows a critical correlation with temperature. Therefore, selecting the appropriate temperature is important in preparing fried shallot oil (FSO). Volatile compounds from five different FSOs were identified and comparatively studied using gas chromatography-mass spectrometry (GC-MS) coupled with multivariate data analysis, including principal component analysis (PCA) and orthogonal partial least squares discriminant analysis (OPLS-DA). GC-MS results identified a total of 93 volatiles, among which aldehydes, alcohols, pyrazines, and sulfur-containing compounds were the major compounds. Eighteen compounds had odor active values (OAV) >1. Among the compounds, hexanal, (E)-2-heptenal, (E)-2-octenal, dipropyl disulfide, 2-ethyl-3,5-dimethylpyrazine, and 1-octen-3-ol were important to the overall aroma profile of FSOs. In the PCA model, all the detected FSOs were divided into three clusters, which were assigned as cluster A (FSO5), B (FSO4), and C (the rest FSOs). Multivariate data analyses revealed that nonanal, 2-ethyl-5-methylpyrazine, (E,E)-2,4-decadienal, (E)-2-heptenal, and hexanal contributed positively to the classification of different FSOs. GC-MS coupled with multivariate data analysis could be used as a convenient and efficient analytical method to classify raw materials.


Assuntos
Culinária , Análise de Alimentos/métodos , Cromatografia Gasosa-Espectrometria de Massas , Óleos Vegetais/química , Cebolinha Branca/química , Temperatura , Compostos Orgânicos Voláteis/análise , Análise Multivariada , Odorantes/análise , Paladar
12.
J Exp Bot ; 2020 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-33258902

RESUMO

Development of leaf margins is an important process in leaf morphogenesis. CIN-clade TCP (TEOSINTE BRANCHED1/CYCLOIDEA/PCF) transcription factors have redundant roles in specifying leaf margins, but the specific mechanisms that individual TCP genes function through remain elusive. Here we report that the CIN-TCP gene TCP5 represses the initiation and outgrowth of leaf serrations by activating two key regulators of margin development, the Class II KNOX factor KNAT3 and BEL-like gene SAW1. Specifically, TCP5 directly promotes the transcription of KNAT3 and indirectly activates the expression of SAW1.Furthermore, we also show that TCP5 regulates KNAT3 and SAW1 in a temporal and spatial specific manner that is largely in accordance with the progress of serration formation. This regulation may serve as a key mechanism to pattern margin morphogenesis and sculpt the final form of the leaf.

13.
Ying Yong Sheng Tai Xue Bao ; 31(9): 3173-3186, 2020 Sep 15.
Artigo em Chinês | MEDLINE | ID: mdl-33345519

RESUMO

Clarifying the consumption of local natural capital stock and natural capital flow and its influencing factors can help alleviate the tension between the socio-economic development of the coastal zone, natural resources, and the ecological environment, and promote regional coordinated and sustainable development. Based on the two-dimensional and three-dimensional ecological footprint evaluation models, we analyzed the spatial and temporal variations of ecological footprint in the coastal zone of Zhejiang Province from 2006 to 2016, and explored the regional natural capital stock and capital flow occupancy based on footprint depth and footprint width. We analyzed the spatial and temporal heterogeneity of its influencing factors using a geographically and temporally weighted regression model. The results showed that ecological crisis in the coastal zone of Zhejiang Province deepened during the study period. The average per capita ecological deficit was 3.5 hm2·cap-1, and the per capita ecological deficit increased by 15.5%. Among them, the contribution of energy consumption account to the per capita ecological footprint was greater than that of the biological resources account. The per capita ecological footprint of each county showed an increasing trend, with significant spatial variation. The coastal zone of Zhejiang Province mainly consumed the natural capital stock, with a significant inhibitory effect on the renewal of natural capital flows. The consumption of natural capital stock was 14.87 times that of capital flow in 2016. Urban scale, economic development level, and people's income level had positive impacts on the ecological footprint of coastal counties of Zhejiang Province, while fishery production had negative effect on the ecological footprints, with transition trend to a positive one. The influence mechanism of the three factors had significant spatial and temporal differentiation.


Assuntos
Conservação dos Recursos Naturais , Desenvolvimento Econômico , China , Humanos , Modelos Teóricos , Desenvolvimento Sustentável
14.
Medicine (Baltimore) ; 99(45): e23070, 2020 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-33157967

RESUMO

INTRODUCTION: Thyroid metastasis from clear cell renal cell carcinoma (ccRCC) is a very rare condition, and its ultrasonographic characteristics have not been summarized in the literature. We herein report a case of thyroid metastasis from ccRCC that occurred 11 years after the surgery and the ultrasonographic characteristics of it are described. PATIENT CONCERNS: A 57-year-old male patient was admitted to our hospital in September 2018 due to discomfort in the neck. No other abnormalities were found during laboratory examination of thyroid function. The previous medical history of the patient included a right nephrectomy for the treatment of ccRCC in June 2007. DIAGNOSIS: Ultrasound examinations revealed multiple thyroid nodules. After nephrectomy, there was no obvious abnormality in the right renal area. Computed tomography (CT) showed an oval lesion with slightly lower density in the right lobe of the thyroid, and the patient was initially diagnosed with nodular goiter. INTERVENTIONS: Bilateral partial thyroidectomy under general anesthesia was conducted. Intraoperative frozen pathological examination showed clear cell carcinoma in the right lobe of the thyroid gland. Therefore, total thyroidectomy and lymph node dissection in the central neck area were performed. OUTCOMES: The patient underwent surgical treatment and recovered successfully. The patient was followed up for 2 years with no further metastasis. CONCLUSION: Ultrasound examination is a safe and convenient screening method. For patients with a renal malignant tumor, if the ultrasound image of thyroid nodule is found to have the characteristics of malignant tumors, the occurrence of metastasis of renal cancer to the thyroid should be highly suspected. Core needle biopsy (CNB) and histopathological diagnosis should be conducted subsequently for early diagnosis.


Assuntos
Carcinoma de Células Renais/diagnóstico por imagem , Carcinoma de Células Renais/secundário , Neoplasias Renais/patologia , Neoplasias da Glândula Tireoide/diagnóstico por imagem , Neoplasias da Glândula Tireoide/secundário , Humanos , Masculino , Pessoa de Meia-Idade , Ultrassonografia
15.
J Nat Prod ; 83(11): 3471-3475, 2020 Nov 25.
Artigo em Inglês | MEDLINE | ID: mdl-33180498

RESUMO

Penisarins A (1) and B (2), sesquiterpene coumarins with an unusual tricyclic sesquiterpene system, were isolated from endophytic Penicillium sp. KMU18029. Their structures were elucidated on the basis of spectroscopic methods, single-crystal X-ray diffraction, and electronic circular dichroism calculations. Compound 2 showed significant cytotoxicities against two human cancer cell lines, HL-60 and SMMC-7721, with IC50 values of 3.6 ± 0.2 and 3.7 ± 0.2 µM, respectively.

16.
Artigo em Inglês | MEDLINE | ID: mdl-33151634

RESUMO

BACKGROUND: Individuals affected by empty nose syndrome secondary to turbinate-sparing techniques (ENS-type) experience decreased productivity and lifestyle disruption owing to considerable nasal-associated discomfort. This study aimed to evaluate the effect of psychosomatic intervention on ENS-type. METHODS: A prospective self-controlled study was conducted, and 28 patients suffering from ENS-type who met the diagnostic criteria for somatic symptom disorder (SSD) according to the Diagnostic and Statistical Manual of Mental Disorders, 5th edition (DSM-5) received cognitive and behavioral therapy (CBT) plus antidepressants. Nasal symptom burden was evaluated using the 25-item Sino-Nasal Outcome Test (SNOT-25). Somatic symptom burden, anxiety severity, and depression severity were assessed by the 25-item Patient Health Questionnaire (PHQ-15), the 9-item PHQ (PHQ-9), and the 7-item Generalized Anxiety Disorder (GAD-7) scale, respectively. Patient assessments were completed prior to treatment and 3 and 12 months after the intervention. RESULTS: The total scores of the SNOT-25 declined posttreatment, showing a significant difference at the 3-month and 12-month follow-ups compared with the baseline scores (p < 0.001). The severity of the 5 most common subjective symptoms, including "nose is too open," "waking up at night," "lack of a good night's sleep," "difficulty falling asleep," and "reduced concentration," declined significantly at 3 and 12 months posttreatment compared to baseline levels. Statistically significant changes in the PHQ-15, PHQ-9, and GAD-7 scores were observed at 3 and 12 months posttreatment compared to baseline scores (p < 0.001). CONCLUSION: This study showed that some patients with ENS-type meeting the diagnostic criteria for SSD might benefit from psychiatric treatment.

17.
Artigo em Inglês | MEDLINE | ID: mdl-33235026

RESUMO

Long noncoding RNAs (lncRNAs) could participate in the development of atherosclerosis (AS). However, the underlying mechanism by which lncRNA H19 is implicated in AS remains largely unknown. In this study, we investigated the function of H19 on cell proliferation, migration and invasion in oxidized low-density lipoprotein (ox-LDL)-treated human aortic vascular smooth muscle cells (HA-VSMC), and on hyperlipidemia response in high-fat diet (HFD)-treated ApoE mice. Moreover, we explored the target interaction among H19, microRNA (miR)-599 and pappalysin 1 (PAPPA). Our results showed that H19 expression was elevated in serum samples of AS patients and ox-LDL-treated HA-VSMC. H19 silence mitigated ox-LDL-induced proliferation, migration and invasion of HA-VSMC. H19 acted as a sponge for miR-599, and miR-599 knockdown reversed the suppressive effect of H19 silence on proliferation, migration and invasion of HA-VSMC. PAPPA was a target of miR-599, and attenuated the inhibitive role of miR-599 in HA-VSMC processes. H19 knockdown repressed PAPPA expression by increasing miR-599. Moreover, H19 interference alleviated hyperlipidemia response in HFD-treated ApoE mice. Collectively, knockdown of H19 inhibited proliferation, migration and invasion of ox-LDL-treated HA-VSMC and hyperlipidemia response in HFD-treated ApoE mice by regulating miR-599/PAPPA axis, indicating H19 might act as a potential target for the treatment of AS.

18.
Int J Mol Med ; 2020 Nov 23.
Artigo em Inglês | MEDLINE | ID: mdl-33236140

RESUMO

Intralesional injection of bleomycin­A5 (BLE­A5) is a novel treatment for nasal polyps. Our previous study clarified that BLE­A5 could induce nasal polyp­derived fibroblast (NPDF) apoptosis in nasal polyps. However, the detailed mechanisms are still unclear. The present study aimed to determine the effects of BLE­A5 on NPDF mitochondrial dynamics and provide a theoretical basis for the local application of BLE­A5 to treat nasal polyps. In the present study, an in vitro nasal polyp tissue culture model was used to define the BLE­A5 target cell type in nasal polyps. NPDF primary cell culture was used to study the effects of BLE­A5 on the mitochondrial dynamic­related mechanism. The results showed that BLE­A5 treatment of NPDFs caused mitochondrial­mediated apoptosis. Dynamin­related protein 1 (Drp1) was shown to be altered in BLE­A5­treated NPDFs. Drp1 knockdown increased the sensitivity of NPDFs to BLE­A5 and exacerbated mitochondrial dysfunction. BLE­A5 decreased cyclin B1­CDK1 complex­mediated phosphorylation of Drp1 and inhibited Drp1­mediated mitophagy in NPDFs. Overall, the present study concluded that BLE­A5 mainly induces NPDF apoptosis in nasal polyps. BLE­A5 regulates the mitochondria by inhibiting Drp1 activation, resulting in NPDF mitochondrial dynamic disorder and apoptosis.

19.
Artigo em Inglês | MEDLINE | ID: mdl-33004280

RESUMO

OBJECTIVE: Thoracic aortic dissection (TAD) is associated with matrix changes, biochemical changes, and inflammatory markers like interleukin-1 beta (IL-1ß). However, the exact mechanism remains unknown. This study aimed to investigate the role of IL-1ß, matrix metalloproteinase (MMP)-2, MMP-9, smooth muscle cell apoptosis, and elastic fibre fracture in the development of TAD in a rat model. METHODS: The TAD rat model was induced by ß-aminopropionitrile (BAPN). TAD was investigated in 112 male Sprague-Dawley rats, which were equally divided into four groups of 28 rats (Control, BAPN, BAPN + IL-1ß, and BAPN + IL-1ß antibody). Systolic blood pressure, survival, and the development of TAD were measured after six weeks. Expression of IL-1ß, MMP-2, and MMP-9 was measured by Western blot. Apoptosis, aortic elastin concentration, and biomechanical characteristics were measured by the TdT mediated dUTP nick end labelling assay, Victoria blue staining, and in vitro testing. RESULTS: During six weeks, the mortality was 0% (0/28) in the control group, 53.6% (15/28) in the BAPN group (p < .001 compared with the control group), 75.0% (21/28) in the BAPN + IL-1ß group (p = .007 compared with the BAPN group), and 35.7% (10/28) in the BAPN + IL-1ß antibody group (p = .023 compared with BAPN group and p < .001 compared with the BAPN + IL-1ß group). IL-1ß treatment deteriorates BAPN induced mortality and aneurysm expansion, which were attenuated by anti-IL-1ß treatment. In BAPN + IL-1ß group, stress and strain parameters were decreased by 13.5%-53.5% and elastin content was decreased by 14%, and IL-1ß, MMP-2, and MMP-9 were expressed higher by 117%, 108%, and 75% when compared with the rats in the BAPN group. Contrarily, in the BAPN + IL-1ß antibody group, the above changes could be completely (strain, elastin content, and expression of MMP-2) or partly (elasticity modulus, stress, and expression of MMP-9) blocked by anti-IL-1ß treatment. CONCLUSION: IL-1ß plays a critical role in TAD formation by altering the expression of MMP-2 and MMP-9, degrading the aortic wall matrix, causing elastic fibre rupture, and changing the stress or strain of the aortic wall. Anti-IL-1ß reduces the later effects and could be one of the molecular targets for prognosis and drug treatment of TAD in the future.

20.
New Phytol ; 2020 Oct 28.
Artigo em Inglês | MEDLINE | ID: mdl-33111313

RESUMO

In grasses, two types of phased, small interfering RNAs (phasiRNAs) are expressed largely in young, developing anthers. They are 21 or 24 nucleotides (nt) in length and are triggered by miR2118 or miR2275, respectively. However, most of their functions and activities are not fully understood. We performed comparative genomic analysis of their source loci (PHAS) in five Oryza genomes and combined this with analysis of high-throughput sRNA and degradome datasets. In total, we identified 8216 21-PHAS and 626 24-PHAS loci. Local tandem and segmental duplications mainly contributed to the expansion and supercluster distribution of the 21-PHAS loci. Despite their relatively conserved genomic positions, PHAS sequences diverged rapidly, except for the miR2118/2275 target sites, which were under strong selection for conservation. We found that 21-nt phasiRNAs with a 5'-terminal uridine (U) demonstrated cis-cleavage at PHAS precursors, and these cis-acting sites were also variable among close species. miR2118 could trigger phasiRNA production from its own antisense transcript and the derived phasiRNAs might reversibly regulate miR2118 precursors. We hypothesised that successful initiation of phasiRNA biogenesis is conservatively maintained, while phasiRNA products diverged quickly and are not individually conserved. In particular, phasiRNA production is under the control of multiple reciprocal regulation mechanisms.

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