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1.
J Appl Microbiol ; 2021 Jul 03.
Artigo em Inglês | MEDLINE | ID: mdl-34216530

RESUMO

AIMS: The study aims to identify a novel plant growth-promoting bacteria (PGPB), which contributes to promoting growth and reducing cadmium (Cd) concentration in rice under Cd-contaminated conditions. METHODS AND RESULTS: Nine bacterial strains were isolated from plants grown in Cd-contaminated soil. These bacteria were tolerant to 1000 µmol l-1 CdCl2 , capable of producing indole-3-acetic acid (IAA), fixing nitrogen, and solubilizing phosphate. The result of hydroponic experiment showed that under the control and Cd stress conditions, the dry weight of the Tm02 inoculated rice seedlings increased significantly. Furthermore, under Cd stress, the concentration of Cd in the shoot of the Tm02 inoculated seedlings decreased significantly, while there was no significant difference in Cd concentration between treatment with other eight strains and noninoculated seedlings. The same results were observed in the pot experiment as well, where there was a significantly reduced Cd concentration in rice grains of the Tm02-inoculated rice plants. Tm02 was classified as Pantoea agglomerans through 16S rDNA sequencing. CONCLUSIONS: A novel PGPB strain Tm02 was identified and confirmed that it has the function of promoting rice growth and reducing Cd concentration in rice grain under Cd-contaminated conditions. This strain has the potential to improve rice yield in Cd-contaminated paddy fields. SIGNIFICANCE AND IMPACT OF THE STUDY: This study provides a new example of using PGPB to improve the tolerance of rice to Cd pollution.

2.
Adv Mater ; : e2101714, 2021 Jul 24.
Artigo em Inglês | MEDLINE | ID: mdl-34302390

RESUMO

2D Ruddlesden-Popper perovskites exhibit great potential in optoelectronic devices for superior stability compared with their 3D counterparts. However, to achieve a high level of device performance, it is crucial but challenging to regulate the phase distribution of 2D perovskites to facilitate charge carrier transfer. Herein, using a solvent additive method (adding a small amount of dimethyl sulfoxide (DMSO) in N,N-dimethylformamide (DMF)) combined with a hot-casting process, the phase distribution of (PEA)2 MA3 Pb4 I13 (PEA+  = C6 H5 CH2 CH2 NH3 + , MA+  = CH3 NH3 + ) perovskite can be well controlled and the Fermi level of perovskites along the film thickness direction can achieve gradient distribution. The increased built-in potential, oriented crystal, and improved crystal quality jointly contribute to the high photoresponse of devices in the entire response spectrum range. The optimum device exhibits a characteristic detection peak at 570 nm with large responsivity/detectivity (0.44 A W-1 /3.38 × 1012 Jones), ultrafast response speed with a rise/fall time of 20.8/20.6 µs, and improved stability. This work suggests the possibility of manipulating the ordered phase distribution of 2D perovskites toward high-performance and stable optoelectronic conversion devices.

3.
J Mol Cell Biol ; 2021 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-34289037

RESUMO

The prevailing coronavirus disease (COVID-19) caused by a novel severe acute respiratory syndrome coronavirus (SARS-CoV-2) has presented some neurological manifestations including hyposmia, hypogeusia, headache, stroke, encephalitis, Guillain‒Barre syndrome, and some neuropsychiatric disorders. Although several cell types in the brain express angiotensin converting enzyme-2 (ACE2), the main SARS-CoV-2 receptor, and other related proteins, it remains unclear whether the observed neurological manifestations are attributed to virus invasion into the brain or just comorbidities caused by dysregulation of systemic factors. Here, we briefly review the neurological manifestations of SARS-CoV-2, summarize recent evidence for the potential neurotropism of SARS-CoV-2, and discuss the potential mechanisms of COVID-19-associated neurological diseases.

4.
J Proteomics ; 246: 104317, 2021 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-34217887

RESUMO

Wheat intolerance has various systemic manifestations that can affect people's quality of life, and few studies have focused on the mechanism of wheat intolerance and the signaling pathways involved in wheat intolerance have not been fully identified. We compared the protein profiles of patients with wheat intolerance with those of healthy controls using LASSO (least absolute shrinkage and selection operator) and PLS (partial least squares regression) to obtain DEPs (differentially expressed proteins) for GO (Gene Ontology) analysis, KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment analysis, and PPI (protein-protein interaction) network analysis. Internal validation and external validation were conducted for target proteomics testing. The correlation between differently expressed protein and the wheat-specific IgG antibody concentration was analyzed. Then ROC curve (receiver operating characteristic curve) was generated to validate the differentially expressed proteins. We identified 33 DEPs as significant candidate proteins of wheat intolerance. These proteins were mainly enriched in complement and coagulation cascade pathways, immune activation, and immune response-related pathways. After internal and external target proteomics validation, CFHR3 (complement factor H-related protein 3) was identified as a key protein that may have an important role in wheat intolerance. We found CFHR3 protein expression abundance and the wheat-specific IgG antibody concentration were significantly negatively correlated (P = 0.035; Spearman correlation coefficient r = -0.565). The AUC (median area under the ROC curve) of CFHR3 is 0.857 in external verification data. This study provides insights into wheat intolerance that can be used to further explore the pathogenesis of this condition. SIGNIFICANCE: Proteomics has performed important potential in food allergy research and is conducive to improving our comprehension on molecular mechanisms of food allergy. The present study identified significant signaling pathways and differentially expressed proteins in patients with wheat intolerance by means of bioinformatics from the viewpoint of mass spectrometry-based proteomics, which provided insights into further research on the pathogenesis and timely diagnosis of wheat intolerance.

5.
Ann Palliat Med ; 10(6): 6351-6358, 2021 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-34237958

RESUMO

BACKGROUND: To investigate the clinical values and relationships of bone turnover markers (BTMs), dual-energy X-ray absorptiometry (DXA), and quantitative computed tomodensitometry (QCT) in the screening of osteoporosis (OP) in elderly Chinese males. METHODS: General data, including age, height, and weight, the results of BTM measurements, and the findings of DXA and QCT in 357 male patients aged ≥50 years who visited the outpatient or inpatient Department of Geriatrics, Beijing Jishuitan Hospital from June 2017 to May 2019 were retrospectively analyzed. RESULTS: The OP detection rates based on T-scores of DXA L1-4, DXA total hip, and spine QCT were 3.4% (12/357), 13.2% (47/357), and 40.3% (144/357), respectively. QCT had a significantly higher OP detection rate than did DXA (P<0.001). There were 24 cases of fragility fractures, which were significantly correlated with the DXA total hip BMD and its T-score, with risk cut-off values of 0.607 g/cm2 and -2.950, respectively. The measured levels of the 5 BTMs were as follows: total procollagen type I amino-terminal propeptide (tPINP), 39.23±20.82 ng/mL; ß-isomerized C-terminal telopeptides (ß-CTX), 0.38±0.21 ng/mL; osteocalcin (OC), 13.50±8.80 ng/mL; 25-hydroxycholecalciferol (25(OH)D3), 12.90±7.46 ng/mL; and parathormone (PTH), 54.50±25.35 pg/mL. The elevation of tPINP, ß-CTX, and OC were negatively correlated with aging and positively correlated with decreased BMD (all P<0.05). OC and 25(OH)D3 values were significantly lower than their normal range. Among the 43 patients with normal bone mass on both DXA and QCT examinations, 34 presented with abnormal BTMs, including elevated tPINP in 2 cases, elevated ß-CTX in 2 cases, and OC decreased in 31 cases. CONCLUSIONS: In the Chinese elderly male population, spine QCT has a higher detection rate of OP than DXA, whereas hip DXA is more advantageous in predicting the risk of fragility fracture. tPINP, ß-CTX, and OC can be used as reliable indicators for the dynamic observation of bone content changes and may screen for early bone metabolism abnormalities when BMD examinations still show negative results.


Assuntos
Densidade Óssea , Remodelação Óssea , Absorciometria de Fóton , Idoso , China , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos
6.
BMC Musculoskelet Disord ; 22(1): 608, 2021 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-34229632

RESUMO

BACKGROUND: Venous thromboembolism (VTE) is a significant complication after joint arthroplasty. Diabetes is related to a few changes in coagulation and fibrinolysis that may lead to thrombophilia. We aimed to investigate the incidence of postoperative VTE and associated risk factors among patients with diabetes undergoing total hip (THA) or total knee anthroplasty (TKA) in a single centre in China. METHODS: Patients with diabetes who underwent THA or TKA from January 2016 to December 2018 (n = 400) at Beijing Jishuitan Hospital were recruited in this study. Lower limb venous Doppler ultrasound was performed before and after surgery to confirm deep venous thrombosis (DVT). Computer tomography pulmonary angiography was done to confirm pulmonary embolism (PE) for those with new postoperative DVT and typical symptoms of PE. A multivariate logistic regression model was conducted to examine factors associated with the development of postoperative VTE. RESULTS: The overall incidence of postoperative VTE in patients with diabetes after THA or TKA was 46.8 % (187 out of 400). Among the 187 VTE patients, 7.5 % (14 out of 187) had proximal vein thrombosis and 92.5 % (173 out of 187) had distal vein thrombosis. No PE occurred. Female patients and patients undergoing TKA had higher incidence of postoperative VTE. Patients who developed postoperative VTE were older, and had higher levels of preoperative D-Dimer and Caprini score. A high level of preoperative D-dimer (OR = 2.11, 95 %CI = 1.35-3.30) and the surgery of TKA (OR = 2.29, 95 %CI = 1.29-4.01) significantly increased the risk of developing postoperative VTE. Postoperative initiation of concomitant mechanical prophylaxis and low molecular weight heparin (LMWH) was protective for postoperative VTE (OR = 0.56, 95 %CI = 0.37-0.86). CONCLUSIONS: VTE is common in patients with diabetes undergoing joint arthroplasty. Patients undergoing TKA or with a high level of preoperative D-dimer are at a considerable risk of developing postoperative VTE. There may be a protective role of postoperative initiation of concomitant mechanical prophylaxis and LMWH for VTE.


Assuntos
Artroplastia de Quadril , Artroplastia do Joelho , Diabetes Mellitus , Tromboembolia Venosa , Anticoagulantes , Artroplastia de Quadril/efeitos adversos , Artroplastia do Joelho/efeitos adversos , China/epidemiologia , Feminino , Heparina de Baixo Peso Molecular , Humanos , Complicações Pós-Operatórias/diagnóstico por imagem , Complicações Pós-Operatórias/epidemiologia , Complicações Pós-Operatórias/etiologia , Fatores de Risco , Tromboembolia Venosa/diagnóstico por imagem , Tromboembolia Venosa/epidemiologia
7.
Br J Radiol ; 94(1124): 20201088, 2021 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-34260297

RESUMO

OBJECTIVE: The aim of this study was to compare the clinical efficacy of neoadjuvant chemoradiotherapy (NACRT) combined with postoperative adjuvant XELOX (Oxaliplatin +Capecitabine) chemotherapy and postoperative adjuvant chemotherapy (ACT) with XELOX for local advanced gastric cancer (LAGC). METHODS: In this prospectively randomized trial, we investigated the effect of NACRT combined with postoperative ACT for LAGC. 60 patients were randomly divided into NACRT group and ACT group, with 30 patients in each group. Patients in NACRT group were given three-dimensional conformal radiotherapy (45 Gy/1.8 Gy/f) accompanied by synchronous XELOX of two cycles, followed by surgery, and then postoperative adjuvant XELOX chemotherapy of four cycles was performed. Patients in ACT group received surgery in advance, and then XELOX chemotherapy of six cycles was given. RESULTS: The objective response rate of NACRT was 76.7%. The overall incidence of postoperative complications in NACRT group was not significantly different from that in ACT group (23.1% vs 30.0%, p = 0.560). The 1 year, 2 years, and 3 years progression-free survival (PFS)and overall survival (OS) in NACRT and ACT groups were 80.0% vs 56.7%, 73.3% vs 46.7%, 60.0% vs 33.3%, and 86.7% vs 80.0%, 76.7% vs 66.7%, 63.3% vs 50.0%, respectively. Patients in NACRT group showed a significantly higher R0 resection rate (84.6% vs 56.7%, p = 0.029),lower loco-regional recurrence rate (36.7% vs 11.5%, p = 0.039), longer PFS (p = 0.019) and freedom from locoregional progression(FFLP) (p = 0.004) than patients in ACT group, while there was no difference in OS (p = 0.215) and in toxicity incidence (p > 0.05). CONCLUSIONS: NACRT combined with postoperative adjuvant XELOX chemotherapy can improve R0 resection rate, reduce loco-regional recurrence, prolong PFS and FFLP without increasing the incidence of postoperative complications in patients with LAGC. ADVANCES IN KNOWLEDGE: Compared with postoperative adjuvant chemotherapy, locally advanced gastric cancer patients may benefit from neoadjuvant chemoradiotherapy, and toxicity associated with chemoradiotherapy was tolerant and manageable.

8.
Oncogene ; 2021 Jul 10.
Artigo em Inglês | MEDLINE | ID: mdl-34247189

RESUMO

The prognosis of hepatocellular carcinoma (HCC) remains unsatisfactory due to limited effective treatment options. In this work, we investigated the therapeutic efficacy of Terbinafine for HCC and the underlying mechanism. The influence of Terbinafine on cell growth, 3D spheroid formation, clonogenic survival, and protein synthesis was investigated in human HCC cell lines. Co-immunoprecipitation, immunofluorescence, and other techniques were employed to explore how Terbinafine exerts its anticancer effect. Subcutaneous tumorigenicity assay, orthotopic and patient-derived xenograft (PDX) HCC models were used to evaluate the anticancer effect of Terbinafine monotherapy and the combinatorial treatment with Terbinafine and sorafenib against HCC. The anticancer activity of Terbinafine was Squalene epoxidase (SQLE)-independent. Instead, Terbinafine robustly suppressed the proliferation of HCC cells by inhibiting mTORC1 signaling via activation of AMPK. Terbinafine alone or in combination with sorafenib delayed tumor progression and markedly prolonged the survival of tumor-bearing mice. The synergy between Terbinafine and sorafenib was due to concomitant inhibition of mTORC1 and induction of severe persistent DNA double-strand breaks (DSBs), which led to the delayed proliferation and accelerated cell death. Terbinafine showed promising anticancer efficacy in preclinical models of HCC and may serve as a potential therapeutic strategy for HCC.

9.
Artigo em Inglês | MEDLINE | ID: mdl-34279751

RESUMO

OBJECTIVE: The purpose of this study was to determine whether the epidermal growth factor receptor (EGFR), which is a classical receptor tyrosine kinase, is involved in the protective effect of morphine against ischemia/reperfusion (I/R)-induced myocardial mitochondrial damage. METHODS: Isolated rats hearts were subjected to global ischemia followed by reperfusion. Cardiac H9c2 cells were exposed to a simulated ischemia solution followed by Tyrode's solution to induce hypoxia/reoxygenation (H/R) injury. Triphenyltetrazolium chloride (TTC) was used to measure infarct size. The mitochondrial morphological and functional changes were determined using transmission election microscopy (TEM), mitochondrial stress assay, and mitochondrial swelling, respectively. Mitochondrial fluorescence indicator JC-1, DCFH-DA, and Mitosox Red were used to determine mitochondrial membrane potential (△Ψm), intracellular reactive oxygen species (ROS) and mitochondrial superoxide. A TUNUL assay kit was used to detect the level of apoptosis. Western blotting analysis was used to measure the expression of proteins. RESULTS: Treatment of isolated rat hearts with morphine prevented I/R-induced myocardial mitochondrial injury, which was inhibited by the selective EGFR inhibitor AG1478, suggesting that EGFR is involved in the mitochondrial protective effect of morphine under I/R conditions. In support of this hypothesis, the selective EGFR agonist epidermal growth factor (EGF) reduced mitochondrial morphological and functional damage similarly to morphine. Further study demonstrated that morphine may alleviate I/R-induced cardiac damage by inhibiting autophagy but not apoptosis. Morphine increased protein kinase B (Akt), extracellular regulated protein kinases (ERK) and signal transducer and activator of transcription-3 (STAT-3) phosphorylation, which was inhibited by AG1478, and EGF had similar effects, indicating that morphine may activate Akt, ERK, and STAT-3 via EGFR. Morphine and EGF increased intracellular reactive oxygen species (ROS) generation. This effect of morphine was inhibited by AG1478, indicating that morphine promotes intracellular ROS generation by activating EGFR. However, morphine did not increase ROS generation when cells were transfected with siRNA against EGFR. In addition, EGFR activity was markedly increased by morphine, but the effect of morphine was reversed by naltrindole. These results suggest that morphine may activate EGFR via δ-opioid receptor activation. CONCLUSIONS: Morphine may prevent I/R-induced myocardial mitochondrial damage by activating EGFR through δ-opioid receptors, in turn increasing RISK and SAFE pathway activity via intracellular ROS. Moreover, morphine may reduce myocardial injury by regulating autophagy but not apoptosis.

10.
Spine (Phila Pa 1976) ; 46(15): 1033-1038, 2021 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-34228695

RESUMO

STUDY DESIGN: Retrospective observational study. OBJECTIVE: To assess the accuracy and reliability of standing lateral lumbar radiographs for measurements of spinopelvic parameters, compared with whole-spine EOS® images. SUMMARY OF BACKGROUND DATA: Lateral lumbar radiographs are commonly used for measurements of spinopelvic parameters. However, variable magnifications by fan-beam x-ray projection at margins may cause measurement errors. METHODS: Fifty consecutive patients with standing lateral lumbar radiographs and whole-spine EOS® images were retrospectively reviewed from March to July in 2019. Two orthopedic surgeons (observers) independently measured the spinopelvic parameters including pelvic incidence (PI), pelvic tilt (PT), sacral slope (SS), and lumbar lordosis (LL) on the computers. These spinopelvic parameters of each patient were measured twice on both lateral lumbar radiograph and EOS® image by two observers with the usage of Surgimap software. The measurement difference and agreement of each parameter value between lateral lumbar radiographs and EOS® images were analyzed by paired t test and the interclass correlation coefficient (ICC) respectively. Intra- and inter-observer's reliabilities of both imaging methods were assessed by ICC. RESULTS: The measurement difference of each spinopelvic parameter on EOS® images and lateral lumbar radiographs was less than 1° on average. Only the measurement difference of PI value revealed statistically significant (P = 0.020) with 0.9° discrepancy (95% confidential interval: 0.2-1.6), which indicated no clinical significance. The ICC values of lateral lumbar radiographs and EOS® images were more than 0.870 (range, 0.872-0.976), thus showed good to excellent measurement agreement between both imaging methods. All the ICC values for evaluating intra- and inter-observer reliability were greater than 0.960 (range, 0.963-0.993), indicating excellent reliability for observer's measurements. CONCLUSION: Measurements of spinopelvic parameters (PI, PT, SS, and LL) on standing lateral lumbar radiographs are accurate and reliable, which are comparable to whole-spine EOS® images.Level of Evidence: 3.


Assuntos
Região Lombossacral , Radiografia/normas , Posição Ortostática , Humanos , Região Lombossacral/anatomia & histologia , Região Lombossacral/diagnóstico por imagem , Reprodutibilidade dos Testes , Estudos Retrospectivos
11.
Biomater Sci ; 2021 Jul 08.
Artigo em Inglês | MEDLINE | ID: mdl-34236351

RESUMO

Correction for 'Anti-osteosarcoma effect of hydroxyapatite nanoparticles both in vitro and in vivo by downregulating the FAK/PI3K/Akt signaling pathway' by Renxian Wang et al., Biomater. Sci., 2020, 8, 4426-4437, DOI: 10.1039/D0BM00898B.

12.
Artigo em Inglês | MEDLINE | ID: mdl-34102052

RESUMO

Development of high-performance materials for electromagnetic wave absorption has attracted extensive interest, but it still remains a huge challenge especially in reducing density and lowering filler loading. Herein, a hierarchical all-carbon nanostructure is rationally designed as follows: the defect-rich hollow graphite capsules (GCs) controlled by the size/density of ZnO templates are synthesized on the surface of helical carbon nanotubes (HCNTs) to form a hybrid nanocomposite, denoted as GCs@HCNTs. As a result, the GCs@HCNTs demonstrate a strong and wide absorption performance with a very low filler loading of 10 wt %. The minimum reflection loss reaches -51.7 dB at 7.6 GHz, and the effective bandwidth (below -10 dB) ranges from 8 to 14 GHz, covering the whole X or Ku bands. The hierarchical nanostructure and homoatomic heterogeneous interface are beneficial to impedance matching and bring additional dipole polarization enhanced by the structural defects, which may enlighten the design of ultralight and broadband high-performance electromagnetic wave absorption materials.

13.
Nanotechnology ; 32(38)2021 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-34144541

RESUMO

This paper describes an investigation into how combined carbon nanotube doping and surface nanostructuring affects the surface properties of polystyrene. Multiwall carbon nanotubes (MWCNTs) have unique anisotropic electrical properties that can be utilized for light absorption, electromagnetic shielding and nanoscale electostatic forces. Polystyrene was doped with 5 wt% MWCNTs and the resulting composite was wetted onto a porous anodic alumina template to form a nanostructure surface of nanotubes. Scanning electron microscopy revealed a hierarchical surface structure with the composite nanotubes bundled together as the MWCNTs increased the attractive forces between the composite nanotubes. Water droplet testing revealed that this hierarchical surface structure was superhydrophobic. Though the presence of the MWCNTs caused a direct increase in absorption, the hierarchical surface structure increased reflection. The addition of 5 wt% of the anionic surfactant Sodium Dodecyl Benzene Sulfonate to ensure MWCNT dispersal did not significantly change hydrophobicity or light absorption despite the hierarchical surface structure becoming finer. The created composite has potential use as a surface layer on an organic surface cell as it provides reduced cleaning needs and electrical disturbance but further work is required to reduce the reflection.

16.
Acta Biochim Biophys Sin (Shanghai) ; 53(8): 988-996, 2021 Jul 28.
Artigo em Inglês | MEDLINE | ID: mdl-34133712

RESUMO

The ubiquitin-proteasome system (UPS) plays a central role in regulating protein homeostasis in tumor progression. The proteasome subunit Rpn10 is associated with the progression of several tumor types. However, little is known regarding the role of Rpn10 in clear cell renal cell carcinoma (ccRCC). In this study, we found that overexpression of Rpn10 increased ccRCC cell proliferation, migration, and invasion. Silencing Rpn10 expression resulted in decreased cell proli-feration, migration, and invasion in ccRCC cells. Knockdown of Rpn10 inhibits tumor growth and cell proliferation in vivo. Furthermore, we demonstrated that Rpn10 increased cell proliferation, migration, and invasion via regulation of the nuclear factor kappa B (NF-κB) pathway. Rpn10 directly promoted inhibitor of nuclear factor-kappa B alpha (IκBα) degradation through the UPS. Moreover, we observed that upregulation of Rpn10 or downregulation of IκBα in ccRCC was associated with poor prognosis. We found that the combination of these two parameters was a more powerful predictor of poor prognosis than either parameter alone. Collectively, these findings provide evidence that Rpn10 promotes the progression of ccRCC by regulation of the NF-κB pathways and is a prognostic indicator for patients with ccRCC.

17.
Braz J Med Biol Res ; 54(7): e10236, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34008750

RESUMO

This work aimed to research the function of MARVEL domain-containing protein 1 (MARVELD1) in glioma as well as its functioning mode. Bioinformatics analysis was utilized to assess the MARVELD1 expression in glioma tissues and its relationship with grade and prognosis, based on The Cancer Genome Atlas (TCGA), Genotype-Tissue Expression (GTEx), and Chinese Glioma Genome Atlas (CGGA) databases. Cell Counting Kit-8 (CCK-8), colony formation, and Transwell assays were carried out to determine the impact of MARVELD1 on malignant biological behavior of glioma, such as proliferation, invasion, and migration. qRT-PCR was carried out to test the mRNA level of MARVELD1. Western blot assay was performed to measure the protein expression of MARVELD1 and JAK/STAT pathway-related proteins. MARVELD1 was expressed at high levels in glioma tissues and cell lines. Kaplan-Meier survival analysis revealed that the higher MARVELD1 expression, the shorter the survival time of patients with glioma. Also, the MARVELD1 expression in WHO IV was significantly enhanced compared to that in WHO II and WHO III. Furthermore, the functional analysis of MARVELD1 in vitro revealed that knockdown of MARVELD1 in U251 cells restrained cell proliferation, migration, and invasion, while up-regulation of MARVELD1 in U87 cells presented opposite outcomes. Finally, we found that JAK/STAT signaling pathway mediated the function of MARVELD1 in glioma. MARVELD1 contributed to promoting the malignant progression of glioma, which is the key driver of activation of JAK/STAT signaling pathway in gliomas.


Assuntos
Neoplasias Encefálicas , Glioma , Animais , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Regulação Neoplásica da Expressão Gênica , Humanos , Proteínas com Domínio MARVEL , Proteínas de Membrana , Camundongos , Camundongos Nus , Proteínas Associadas aos Microtúbulos , Fenótipo , Transdução de Sinais , Regulação para Cima
18.
Nat Commun ; 12(1): 3059, 2021 05 24.
Artigo em Inglês | MEDLINE | ID: mdl-34031390

RESUMO

Non-alcoholic fatty liver disease (NAFLD) has become the most prevalent chronic liver disease in the world, however, no drug treatment has been approved for this disease. Thus, it is urgent to find effective therapeutic targets for clinical intervention. In this study, we find that liver-specific knockout of PPDPF (PPDPF-LKO) leads to spontaneous fatty liver formation in a mouse model at 32 weeks of age on chow diets, which is enhanced by HFD. Mechanistic study reveals that PPDPF negatively regulates mTORC1-S6K-SREBP1 signaling. PPDPF interferes with the interaction between Raptor and CUL4B-DDB1, an E3 ligase complex, which prevents ubiquitination and activation of Raptor. Accordingly, liver-specific PPDPF overexpression effectively inhibits HFD-induced mTOR signaling activation and hepatic steatosis in mice. These results suggest that PPDPF is a regulator of mTORC1 signaling in lipid metabolism, and may be a potential therapeutic candidate for NAFLD.


Assuntos
Fígado Gorduroso/metabolismo , Peptídeos e Proteínas de Sinalização Intracelular/genética , Peptídeos e Proteínas de Sinalização Intracelular/metabolismo , Fígado/metabolismo , Transdução de Sinais/fisiologia , Serina-Treonina Quinases TOR/metabolismo , Animais , Proteínas Culina/metabolismo , Proteínas de Ligação a DNA/metabolismo , Células HEK293 , Células Hep G2 , Humanos , Metabolismo dos Lipídeos , Fígado/patologia , Masculino , Camundongos , Camundongos Knockout , Hepatopatia Gordurosa não Alcoólica/metabolismo
19.
Small ; : e2101301, 2021 May 03.
Artigo em Inglês | MEDLINE | ID: mdl-33939883

RESUMO

Achieving uniform lithium (Li) deposition is the key to tackle uncontrollable dendrite growth, which hinders the application of Li metal anodes. In this study, molten Li is thermally injected into a 3D framework by growing lithiophilic CoO nanosheets on Cu foam (CF). The CoO layer grown on the CF surface physically adsorbs molten Li, which makes it possible to spontaneously wet the framework. The morphology of CoO nanosheets does not change during the Li injection process and formed a multi-level structure with the CF, which is difficult to be achieved previously, as most lithiophilic oxides undergo serious chemical changes due to chemical reaction with Li and cannot provide a stable submicron structure for the subsequent Li stripping/plating process. The super-assembled multi-level structure provides abundant Li nucleation sites and electrolyte/electrode contact areas for rapid charge transfer in the composite anode. Therefore, the prolonged lifespan of symmetrical cells for 300 cycles at 10 and 10 mAh cm-2 with lower polarization is achieved, which further renders the LiFePO4 and Li4 Ti5 O12 based full cells with improved capacity retention up to 87.3% and 80.1% after 500 cycles at 1 C. These results suggest that the composite anode has a great application prospect.

20.
J Phys Chem B ; 125(18): 4667-4680, 2021 05 13.
Artigo em Inglês | MEDLINE | ID: mdl-33938737

RESUMO

To gain insight into the reaction mechanism of activated processes, we introduce an exact approach for quantifying the topology of high-dimensional probability surfaces of the underlying dynamic processes. Instead of Morse indexes, we study the homology groups of a sequence of superlevel sets of the probability surface over high-dimensional configuration spaces using persistent homology. For alanine-dipeptide isomerization, a prototype of activated processes, we identify locations of probability peaks and connecting ridges, along with measures of their global prominence. Instead of a saddle point, the transition state ensemble (TSE) of conformations is at the most prominent probability peak after reactants/products, when proper reaction coordinates are included. Intuition-based models, even those exhibiting a double-well, fail to capture the dynamics of the activated process. Peak occurrence, prominence, and locations can be distorted upon subspace projection. While principal component analysis accounts for conformational variance, it inflates the complexity of the surface topology and destroys the dynamic properties of the topological features. In contrast, TSE emerges naturally as the most prominent peak beyond the reactant/product basins, when projected to a subspace of minimum dimension containing the reaction coordinates. Our approach is general and can be applied to investigate the topology of high-dimensional probability surfaces of other activated processes.


Assuntos
Alanina , Dipeptídeos , Conformação Molecular , Probabilidade
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