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1.
Vasc Endovascular Surg ; : 15385744231154087, 2023 Jan 27.
Artigo em Inglês | MEDLINE | ID: mdl-36705019

RESUMO

PURPOSE: We aimed to assess the safety and efficacy of the double microcatheter technique in the treatment of saccular splenic artery aneurysms. METHODS: From November 2013 to October 2020, 56 patients with saccular splenic artery aneurysms underwent endovascular treatment with the double microcatheter technique at our institution. Technical success was defined as embolization of the aneurysmal cavity with no obstruction of the parent artery. Clinical success was defined as no deaths due to splenic artery aneurysms, and no reintervention, recrudescence or organ dysfunction at 1, 6, and 12 months. RESULTS: The technical success rate was 100%. No major complications related to angiography or embolization were observed. Minor complications included fever, pain, and nausea, and 13 patients developed minor complications. At 1 month, the rate of clinical success was 96.4%. At 6 and 12 months, the clinical success rate was 92.9%. There were no aneurysmal recurrences or necessities of reintervention. CONCLUSIONS: Coil embolization with the double microcatheter technique is a safe and effective modality for treating saccular splenic artery aneurysms and offers a reasonable choice for patients who want to retain their original hemodynamics.

2.
BMC Pulm Med ; 23(1): 1, 2023 Jan 03.
Artigo em Inglês | MEDLINE | ID: mdl-36597085

RESUMO

BACKGROUND: Gender differences in bone metabolism of people with chronic obstructive pulmonary disease (COPD) remain unclear. We aim to explore the characteristics of bone metabolism and its clinical significance for patients with COPD. METHODS: A total of 564 cases (282 COPD cases and 282 controls) were preselected. Clinical and analytical characteristics of these cases were assessed. After excluding patients with other conditions known to disturb calcium metabolism, 333 patients (152 COPD cases and 181 controls) were identified. The medical records, indexes of bone turnover markers, serum calcium and phosphorus of the 333 patients were collected and their correlation was analyzed. RESULTS: The 152 cases with COPD were 82.61 ± 7.745 years, 78.3% males, and the 181 age- and sex-matched control cases were 79.73 ± 11.742 years, 72.4% males. Levels of total procollagen type I amino-terminal propeptide (tPINP), osteocalcin (OC), serum calcium and phosphate were significantly lower (P < 0.001) while the level of parathormone (PTH) was significantly higher (P = 0.004) in COPD than in controls. The 25-hydroxycholecalciferol (25(OH)D3) was below the lower limit of normal value (LLN) in both groups, which was significantly lower in COPD males than in control males (P = 0.026). In COPD group, PTH level was significantly higher in females (P = 0.006), and serum P was lower in males (P = 0.006). The adjusted linear regression analysis showed that the levels of tPINP, OC and serum Ca were decreasing greatly in COPD group [ß (95%CI) - 8.958 (- 15.255 to - 2.662), P = 0.005; - 4.584 (- 6.627 to - 2.542), P < 0.001; - 0.065 (- 0.100 to - 0.031), P < 0.001]. Besides, smoke exposure, gender (male) were also related to hypocalcemia [ß (95%CI) - 0.025 (- 0.045 to - 0.005), P = 0.017; - 0.041 (- 0.083 to - 0.001), P = 0.047], and 25(OH)D3 was correlated with serum calcium, phosphorus, and PTH [ß (95%CI) 15.392(7.032-23.753), P < 0.001; - 7.287 (- 13.450 to - 1.124), P = 0.021; - 0.103(- 0.145 to - 0.061), P < 0.001], and female was more likely to have secondary hyperparathyroidism [ß (95%CI) 12.141 (4.047-20.235), P = 0.002]. CONCLUSION: COPD patients have remarkably low bone turnover (indicated by OC) and impaired bone formation (low tPINP), and they are also more prone to low calcium. Smoking and male may play roles in the formation of hypocalcemia, and the secondary hyperparathyroidism is more significant in COPD women. There may be gender differences in bone metabolism abnormalities and their mechanisms of COPD. The conclusion above still need further research and demonstration.


Assuntos
Hiperparatireoidismo Secundário , Hipocalcemia , Doença Pulmonar Obstrutiva Crônica , Humanos , Masculino , Feminino , Cálcio/metabolismo , Estudos Transversais , Fatores Sexuais , Hormônio Paratireóideo/metabolismo , Osteocalcina , Fósforo/metabolismo , Biomarcadores
3.
Artigo em Inglês | MEDLINE | ID: mdl-36607153

RESUMO

OBJECTIVE: The aim of the study was to evaluate the long-term efficacy and quality of life concerning SSLF using conventional instruments for Chinese women with symptomatic stage 2 and 3 apical prolapse. METHOD: This single-arm prospective cohort study was conducted at our hospital between October 2011 and December 2018. Eligible participants were followed up over 3 years after surgery. The primary surgical outcome was composite surgical failure. Secondary outcomes included satisfactory rate, quality of life (QoL) scores and long-term complications. RESULTS: Fifty-nine patients were enrolled (mean age, 57.1 years), of whom 55 (93.22%) completed 3-year follow-up. At year 3, the composite failure rate was 21.34% (95%CI 9.30% -31.79%), and satisfactory rate was 81.40% (95%CI 66.09% -91.08%). Right thigh pain and de novo dyspareunia occurred in 1.8% and 14.6% patients after year 1, but at year 3 there was none. 5.5% patients had lower urinary tract symptoms. Improvement in urinary symptoms and prolapse symptoms was found, but sexual function showed no significant change. CONCLUSIONS: Compared with results at year 1, complication rates of SSLF decreased at year 3. The composite failure rate was relatively low and satisfactory rate relatively high at year.3. Prolapse and urinary symptoms improved significantly after surgery.

4.
Adv Sci (Weinh) ; : e2206729, 2023 Jan 16.
Artigo em Inglês | MEDLINE | ID: mdl-36646508

RESUMO

The application of photoelectrochemical (PEC) water splitting is limited by the sluggish surface oxygen evolution reaction (OER) kinetics. OER kinetics can be effectively improved through cocatalyst engineering. However, the tardy transfer process and serious recombination of carriers are the key factors restricting the cocatalyst development. Taking BiVO4 as an example, a Co-modified heme film rich in large conjugated ring structures is introduced onto the photoanode surface using a solvothermal method. This film functions as an efficient cocatalyst. It considerably reduces the surface overpotential, promotes the transfer of photogenerated holes, and boosts the kinetics of OER by specifically affecting the formation of OOH*. Simultaneously, the formed CoOV bonds induce strong interaction at the photoanode/cocatalyst interfaces, reducing the recombination of photogenerated carriers. Consequently, the onset potential of the optimized photoanode decreases from 0.45 to 0.07 V and the photocurrent density at 1.23 V versus reversible hydrogen electrode boosts to 5.3 mA cm-2 . This work demonstrates a facile strategy for designing cocatalysts to obtain rapid hole transfer capability and reduced carrier recombination for improved PEC performance.

5.
Oncol Lett ; 25(2): 69, 2023 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-36644150

RESUMO

In patients with AFP-negative hepatocellular carcinoma (HCC), des-γ-carboxyprothrombin (DCP) is an important prognostic indicator for the preoperative assessment of transarterial chemoembolization (TACE). However, the association between the serum DCP levels and the degree of progression and prognosis of patients with AFP-negative HCC treated with TACE has not been thoroughly investigated to date, and the molecular mechanism is also unclear. The present study retrospectively analyzed the clinical data of 107 patients with AFP-negative HCC treated with TACE and divided them into two groups based on the median serum DCP levels. The association between DCP and the clinical characteristics of the patients was analyzed, and the survival data were analyzed using Kaplan-Meier curves and Cox regression models. The results demonstrated that the median follow-up time was 755 days (range, 64-1,556 days), and patients in the low-DCP group (n=11; 20.8%) had a lower mortality rate than those in the high-DCP group (n=20; 37.0%). Cox multivariate regression analysis suggested that preoperative lymph node metastasis [hazard ratio (HR), 3.903; 95% CI, 1.778-8.519; P=0.001] and DCP group (HR, 2.465; 95% CI, 1.038-5.854; P=0.041) were independent risk factors. Furthermore, the Gene Expression Omnibus database was utilized to screen differentially expressed mRNAs. Enrichment analyses were then performed, and a protein-protein interaction (PPI) network was constructed to identify hub genes. A total of 169 differentially expressed genes were screened. Enrichment analyses revealed that cancer-related and ribosomal pathways were significantly enriched. Furthermore, 10 hub genes were identified in the PPI network by counting the number of gene interactions, the majority of which belonged to the ribosomal protein (RPS) family, and the top three significant genes were RPS23, RPS11 and RPS3A. In patients with AFP-negative HCC, higher serum DCP levels were associated with poor prognosis after TACE. This may be associated with genes such as those belonging to the RPS family, which may contribute to future personalized therapy for this disease.

6.
Sci Total Environ ; 863: 160891, 2023 Mar 10.
Artigo em Inglês | MEDLINE | ID: mdl-36526180

RESUMO

Over half of the Earth's wetlands have been reclaimed for agriculture, leading to significant soil P destabilization and leaching risks. To evaluate the effects of agricultural land use on soil P stability, we used sequential P extraction to investigate the long-term effects of wetland cultivation for rice and soybean on soil P fractions, including labile and moderately labile inorganic/organic P (LPi, LPo, MPi, and MPo), and stable P in Northeast China. The results showed that soybean cultivation decreased the total P by 35.9 %, whereas rice cultivation did not influence the total P content (p < 0.05). Both the soybean and rice cultivations significantly increased LPi (p < 0.05). Soybean cultivation significantly decreased the LPo and MPo compared to rice cultivation, and the latter increased MPi by 309.28 % compared with the reference wetlands (p < 0.05). Redundancy analysis indicated that pH, poorly crystalline Fe (Feca), crystalline Fe (Fec), and total organic carbon (TOC) explained similar variations in P fractions during soybean and rice cultivation (54.9 % and 49.7 %, respectively). Similarly, during soybean or rice cultivation, pH negatively influenced LPo and MPo, while Feca positively influenced MPi and LPi. Furthermore, TOC showed a positive role in LPo, and MPo, but a negative effect on LPi and MPi during rice cultivation. Hence, we concluded that the cultivation of soybean or rice create contrasting modifications to wetland soil P fractionation by altering TOC, Feca, Fec, and pH. Our study indicates that agricultural land use can regulate the fate of wetland soil P fractionation, with potential benefits to both i) P risk management in cultivated wetlands and ii) potential approaches for future wetland restoration.


Assuntos
Oryza , Solo , Solo/química , Áreas Alagadas , Fósforo/análise , Agricultura/métodos , China , Soja , Carbono/análise
7.
J Colloid Interface Sci ; 634: 601-609, 2023 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-36549208

RESUMO

In recent years, branched or star-shaped Au nanostructures composed of core and protruding arms have attracted much attention due to their unique optical properties and morphology. As the clinically adapted nanoagent, prussian blue (PB) has recently gained widespread attention in cancer theranostics with potential applications in magnetic resonance (MR) imaging. In this article, we propose a hybrid star gold nanostructure(Au-star@PB)as a novel theranostic agent for T1-weighted magnetic resonance imaging (MRI)/ photoacoustic imaging(PAI) and photothermal therapy (PTT) of tumors. Importantly, the Au-star@PB nanoparticles function as effective MRI/PA contrast agents in vivo by increasing T1-weighted MR/PAI signal intensity and as effective PTT agents in vivo by decreasing the tumor volume in MCF-7 tumor bearing BALB / c mouse model as well as in vitro by lessening tumor cells growth rate. Interestingly, we found the main photothermal effect of Au-star@PB is derived from Au-star, but not PB. In summary, the hybrid structure of Au-star@PB NPs with good biological safety, significant photostability, dual imaging capability, and high therapeutic efficiency, might offer a novel avenue for the future diagnosis and treatment of cancer.


Assuntos
Nanopartículas , Neoplasias , Camundongos , Animais , Fototerapia/métodos , Nanopartículas/química , Ferrocianetos/química , Imageamento por Ressonância Magnética/métodos , Neoplasias/diagnóstico por imagem , Neoplasias/terapia , Meios de Contraste/química , Camundongos Endogâmicos BALB C , Linhagem Celular Tumoral , Ouro/química
8.
Comput Biol Med ; 152: 106310, 2022 Nov 28.
Artigo em Inglês | MEDLINE | ID: mdl-36462368

RESUMO

Pelvic fracture is the most serious bone trauma and has the highest mortality and disability rate. Surgical treatment of pelvic fracture is very challenging for surgeons. Minimally invasive close reduction of pelvic fracture is considered the most difficult operation due to the complex pelvic morphology and abundant soft tissue anatomy, both of which increase the difficulty of pelvic fracture reduction. The most challenging aspect of such surgery is how to hold the pelvic bone and effectively transmit the reduction force to the bone. Therefore, a safe and effective pelvic holding pathway for reduction is necessary for pelvic fracture operations. Existing research on the pelvic holding pathway addresses anatomical position and dimension. Few studies have focused on biomechanical properties or on surgical techniques related to these pathways. This paper studies the three holding pathways that are most commonly used in clinical practice. The most effective force direction for each holding pathway is identified through finite element modeling. Pathway 1 is suitable for internal rotation operation and open/close-book operation of the pelvis; Pathway 2 is suitable for translation of the fractured pelvis toward the sacrum and internal pelvic rotation operations; Pathway 3 is suitable for pulling and lifting of the fractured pelvis against gravity and open/close-book operation of the pelvis. In addition, we find through our simulation that the use of a combined holding strategy can reduce the reduction force during the reduction process. We compared the performances of the 2-pin combined holding strategy (2P-CH) and the 3-pin combined strategy (3P-CH). During translational reduction, 2P-CH and 3P-CH showed little difference in pelvic reduction force. However, in rotational reduction, 3P-CH shows advantages. It has less reduction force and the least combined muscle resistance. It can also maximize the displacement of the iliac crest under the same conditions. The results of this study can be applied to surgical planning and to the development of robot-assisted surgery systems in selecting holding pathways and operation strategies for fractured pelvis.

9.
Adv Sci (Weinh) ; : e2202448, 2022 Dec 01.
Artigo em Inglês | MEDLINE | ID: mdl-36453576

RESUMO

The guanine nucleotide exchange factor (GEF) SOS1 catalyzes the exchange of GDP for GTP on RAS. However, regulation of the GEF activity remains elusive. Here, the authors report that PPDPF functions as an important regulator of SOS1. The expression of PPDPF is significantly increased in pancreatic ductal adenocarcinoma (PDAC), associated with poor prognosis and recurrence of PDAC patients. Overexpression of PPDPF promotes PDAC cell growth in vitro and in vivo, while PPDPF knockout exerts opposite effects. Pancreatic-specific deletion of PPDPF profoundly inhibits tumor development in KRASG12D -driven genetic mouse models of PDAC. PPDPF can bind GTP and transfer GTP to SOS1. Mutations of the GTP-binding sites severely impair the tumor-promoting effect of PPDPF. Consistently, mutations of the critical amino acids mediating SOS1-PPDPF interaction significantly impair the GEF activity of SOS1. Therefore, this study demonstrates a novel model of KRAS activation via PPDPF-SOS1 axis, and provides a promising therapeutic target for PDAC.

10.
Rev. bras. med. esporte ; 28(6): 745-748, Nov.-Dec. 2022. tab, graf
Artigo em Inglês | LILACS-Express | LILACS | ID: biblio-1376734

RESUMO

ABSTRACT Introduction Aerobic and anaerobic skills are essential characteristics in Kung Fu competitions. Research concerning their repercussions on athletes' body composition has gained emphasis due to increased sports competitiveness. Squat training is used in martial arts, but there are no reports about the results in kung fu practitioners. Objective Investigate the effects of squat training on lower limb strength in Kung Fu athletes. Methods 18 martial arts athletes were randomly divided into dynamic preparation activities with stretching (control), squat and half-squat exercises. Results The paired T-test result of the control subjects was P=0.578, and the paired squat test result, relative to the control group, before and after the experiment was P=0.164. The power gain in the body posture trained with squat was 24.14 z 9.81KG, and in the half squat was 23.10±11.75KG. The strength gain in the body posture with half squat is 29.75±10.79KG versus 20.59±12.59KG in the squat test. Conclusion After six weeks of training, both the squat and half squat groups significantly improved the maximum results of the lower limb strength tests. The muscular strength of athletes is improved throughout the range of motion, reducing the injury risk on the athletes. Evidence Level II; Therapeutic Studies - Investigating the result.


RESUMO Introdução Habilidades aeróbicas e anaeróbicas são características importantes nas competições de Kung Fu. A investigação sobre suas repercussões na composição corporal dos atletas tem ganhado ênfase devido ao aumento da competitividade esportiva. O treino de agachamento é utilizado em artes marciais, porém não há relatos sobre os resultados em praticantes de kung fu. Objetivo Investigar quais são os efeitos do treino de agachamento repercutidos sobre a força dos membros inferiores em praticantes de Kung Fu. Métodos 18 atletas de artes marciais foram divididos aleatoriamente em grupos para atividades dinâmicas de preparação com alongamento (controle), para exercícios de agachamento e para exercícios de meio agachamento, com força máxima. Resultados O resultado do teste T emparelhado dos sujeitos de controle foi P=0,578 e o resultado do teste de agachamento emparelhado, em relação ao grupo controle, antes e depois do experimento foi P=0,164. O ganho de potência na postura corporal treinada com agachamento foi de 24,14 z 9,81KG, e no meio agachamento foi de 23,10±11,75KG. O ganho de força na postura corporal com meio agachamento é de 29,75±10,79KG contra 20,59±12,59KG no teste de agachamento. Conclusão Após seis semanas de treino, tanto o grupo de agachamento e meio agachamento melhoraram significativamente os resultados máximos dos testes de força nos membros inferiores. A força muscular dos atletas é aprimorada em toda a amplitude de movimento, reduzindo o risco de lesões para os atletas. Nível de evidência II; Estudos Terapêuticos - Investigação de Resultados.


RESUMEN Introducción Las capacidades aeróbicas y anaeróbicas son características importantes en las competiciones de Kung Fu. La investigación sobre su repercusión en la composición corporal de los atletas ha ido ganando énfasis debido al aumento de la competitividad deportiva. El entrenamiento de sentadillas se utiliza en las artes marciales, pero no hay informes sobre los resultados en los practicantes de kung fu. Objetivo Investigar cuáles son los efectos del entrenamiento de agachamiento sobre la fuerza de las extremidades inferiores en los practicantes de Kung Fu. Métodos 18 atletas de artes marciales fueron divididos aleatoriamente en grupos para actividades de preparación dinámica con estiramientos (control), para ejercicios de sentadilla y para ejercicios de media sentadilla, con fuerza máxima. Resultados El resultado de la prueba T emparejada de los sujetos de control fue de P=0,578 y el resultado de la prueba de sentadillas emparejada en comparación con el grupo de control antes y después del experimento fue de P=0,164. La ganancia de potencia en la postura corporal entrenada con sentadilla fue de 24,14 z 9,81KG, y en la media sentadilla fue de 23,10±11,75KG. La ganancia de fuerza en la postura corporal con media sentadilla es de 29,75±10,79KG frente a 20,59±12,59KG en la prueba de sentadilla. Conclusión Tras seis semanas de entrenamiento, tanto el grupo de sentadillas como el de medias sentadillas mejoraron significativamente los resultados máximos de las pruebas de fuerza de las extremidades inferiores. La fuerza muscular de los atletas mejora en toda la gama de movimientos, lo que reduce el riesgo de lesiones para los atletas. Nivel de evidencia II; Estudios terapéuticos - Investigación de resultados.

11.
J Steroid Biochem Mol Biol ; 227: 106236, 2022 Dec 21.
Artigo em Inglês | MEDLINE | ID: mdl-36563764

RESUMO

Fungal hydroxylation of steroids is a key step in the industrial production of various steroid drugs. The main enzymes that enable these reactions are Cytochrome P450s (CYP), though very few industrially important CYPs have been identified and characterized. In this study, we identified a CYP enzyme (CYP-N2) and a cytochrome P450 reductase (CPRns) from Nigrospora sphaerica 722 by a combination of transcriptome sequencing and heterologous expression in Pichia pastoris. Gene CYP-N2 co-expressed with CPRns in Pichia pastoris GS115 showed 6ß- and 15α-hydroxylation activities on progesterone. Different hydroxylation specificity of CYP-N2 was observed on different steroid substrates. CYP-N2 showed 1α-hydroxylation on cortisone and 1α-hydroxylation and 6ß-hydroxylation activities on androstenedione (AD). With dehydroepiandrosterone (DHEA) as a substrate, the hydroxylated products of CYP-N2 included 7α-hydroxy-DHEA and 7α,15α-dihydroxy-DHEA. In order to precisely elucidate CYP-N2 biological function and find out the key amino acids influencing its hydroxylation capabilities in the binding pocket, new generation artificial intelligence technology AlphaFold 2 was used to predict the function-structure of CYP-N2 with high reliability. Through molecular docking, it was concluded that the residues almost binding all substrates were located in the same substrate binding pocket and the various hydroxylation abilities might be due to the different binding conformations of different substrates in the binding pocket. Alanine scanning mutagenesis was used to verify key amino acids identified by the molecular docking with steroid substrates. The 128 THR mutation resulted in conversion rate increase for substrates AD and cortisone by 2.6-fold and 2.1-fold respectively. The information obtained in this study is beneficial to facilitating the engineering of more efficient steroid hydroxylases for industrial applications.

12.
Am J Transl Res ; 14(11): 7880-7888, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36505309

RESUMO

OBJECTIVES: To establish an automatic approach for the measurement of the Cobb angle and the diagnosis of scoliosis on chest radiograph. METHODS: We developed an artificial intelligence (AI) automatic program which contained a supervised learning module and an inference module. After the filtering and pre-processing process, 96 images from the Shenzhen chest X-ray set were used for training with the supervised learning module, and 491 test images were separately gauged by the AI and the corresponding manual methods. The results of the two methods were further compared through statistical analyses. RESULTS: Among the test images, 6068 (99.49%) vertebral bodies were identified within the deviation of one vertebral segment. The value difference between the Cobb angle obtained by the AI program and that measured by specialists was 0.4020±0.8703. The intraclass correlation coefficient of 0.915 indicated the strong agreement. AI scoliosis diagnosis achieved an accuracy of 98.37%, with a specificity of 98.73%, a sensitivity of 88.24% and a kappa coefficient of 0.781. And the area under the receiver operating characteristic curve of 0.979 confirmed the consistency of the two methods in diagnosis. CONCLUSIONS: We developed a novel automatic AI method with the abilities to measure the Cobb angle, and to identify the approximate vertebral segment and diagnosis of scoliosis on chest radiograph. The results suggest that this method might be a promising alternative strategy for scoliosis screening on chest radiograph and worth further investigation.

14.
Curr Res Microb Sci ; 3: 100168, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36518161

RESUMO

Wind-wave disturbance and nutrient input are the major environmental factors that shape bacterial diversity and community composition in lakes. However, knowledge of bacteria as bio-indicators' synergistic response to disturbance patterns and nutrient addition is still poor. To address this knowledge gap, we conducted a mesocosm experiment over 21 days, and collected 90 water samples from tanks under six different patterns of disturbance and nutrient addition; we explored the bacterial communities using high-throughput 16S rRNA gene sequencing. We found significantly increased bacterial diversity in continuously disturbed conditions and in intermittently disturbed conditions, and significantly decreased bacterial diversity under the synergistic effect of disturbances and nutrients. Bacterial community composition (BCC) under conditions of disturbance and nutrient addition exhibited different variations. However, there were no significant differences in BCC between continuously and intermittently disturbed mesocosms. Co-occurrence networks analysis showed that the proportion of positive correlations among edges in nutrient addition conditions were significantly higher than that in disturbed condition, indicating that nutrient input may increase the proportion of niche overlap, and enhance positive interactions among bacteria. Meanwhile, the co-occurrence patterns between algal and bacterial communities suggested shared environmental preferences or potential interactions among these two groups. Our study provides a new insight into the restoration of shallow eutrophic lake ecosystems. Moreover, the different distribution patterns of bacterial community act as ecological indicators of response to disturbances and nutrient input.

15.
Molecules ; 27(23)2022 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-36500663

RESUMO

A simple, rapid, sensitive, accurate, and automatic graphite furnace atomic absorption spectrometry (GFAAS) method for detecting Cd and Pb in cereals is presented. This method enables the simultaneous determination of Cd and Pb in cereals with a pre-treatment method of diluted acid extraction and a high-performance lead-cadmium composite hollow-cathode lamp (LCC-HCL), and it realizes automatic determination from sample weighing to result output through an automatic diluted acid extraction system. Under the optimization, Pb and Cd in cereals were simultaneously and automatically detected in up to 240 measurements in 8 h. The LOD and LOQ of this method were 0.012 and 0.040 mg·kg-1 for Pb, and 0.0014 and 0.0047 mg·kg-1 for Cd, respectively. The results of the four certified reference materials were satisfied; there was no significant difference compared with the ICP-MS method according to a t-test, and the RSDs were less than 5% for Cd and Pb. The recoveries of naturally contaminated samples compared with the ICP-MS method were favorable, with 80-110% in eight laboratories. The developed method is rapid, low-cost, and highly automated and may be a good choice for grain quality discrimination and rapid analysis of Cd and Pb in different institutions.


Assuntos
Cádmio , Grafite , Espectrofotometria Atômica/métodos , Cádmio/análise , Grafite/química , Grão Comestível/química , Eletrodos
16.
Sci Rep ; 12(1): 22650, 2022 12 31.
Artigo em Inglês | MEDLINE | ID: mdl-36587111

RESUMO

Adjuvant endocrine therapy improves the prognosis of early breast cancer with hormone receptor positivity. However, there is no systematic report on the effect of endocrine therapy (particularly ovarian function suppression, OFS) on serum lipids in premenopausal women. This retrospective cohort study aimed to determine whether various endocrine treatments had different effects on blood lipids. This study enrolled 160 premenopausal patients with stage I-III breast cancer in eastern China. The initial diagnostic information was retrieved from patient's medical records, including age at the time of diagnosis, tumor characteristics, anticancer treatment and past medical history. The changes in blood lipids in patients receiving different types of endocrine therapy were compared at the 3rd, 6th, 12th, and 24th months after initiating endocrine therapy. Generalized linear mixed model was used in our analyses. Our data revealed that low-density lipoprotein cholesterol (LDL-C) levels in patients with tamoxifen (TAM) were significantly lower in the 6th, 12th, and 24th months than that in the 3rd month, while high-density lipoprotein cholesterol (HDL-C) levels in the 6th, 12th, and 24th months were significantly higher than that in the 3rd month, indicating that blood lipid levels generally improved with time. While in TAM plus OFS group, HDL-C levels were significantly higher in the 24th month than in the 3rd month, total cholesterol (TC) levels were significantly higher in the 24th month than in the 6th month. The lipid profiles of OFS plus aromatase inhibitor (AI) group did not show significant differences at any time point but were significantly higher than those of the other two groups especially in LDL and TC. TAM group tended to have lower serum lipid levels. With longer follow-up, no statistically significant difference in values was observed between TAM and TAM plus OFS groups at various time points. Compared with the other two groups, OFS plus AI group presented an increasing trend toward LDL-C and TC. The risk of dyslipidemia requires further investigation using a large sample size.


Assuntos
Neoplasias da Mama , Feminino , Humanos , Neoplasias da Mama/patologia , Antineoplásicos Hormonais/uso terapêutico , Estudos Retrospectivos , LDL-Colesterol , Quimioterapia Adjuvante , Tamoxifeno/uso terapêutico , Pré-Menopausa
17.
Materials (Basel) ; 15(24)2022 Dec 17.
Artigo em Inglês | MEDLINE | ID: mdl-36556841

RESUMO

In this paper, glass fiber fabric reinforced polyphenylene sulfide composites were prepared by hot pressing. The effects of glass fibre modification and hot pressing temperature on the properties of the composites were investigated using a scanning electron microscope, infrared spectrometer, universal testing machine, and DIGEYE digital imaging colour measurement system. The results show that after the treatment with a silane coupling agent, the silane coupling agent was more uniformly distributed on the surface of the glass fibres, and the bonding effect between the glass fibre fabric and polyphenylene sulphide was significantly improved. The strength of the composites increased and then decreased with the increase of hot pressing temperature, and the surface colour of the composites became darker and darker. When the hot-pressing temperature is 310 °C, the mechanical properties of glass fabric-reinforced polyphenylene sulfide composites are at their best, the tensile strength reaches 51.9 MPa, and the bending strength reaches 78 MPa.

18.
Chem Biodivers ; : e202200800, 2022 Dec 23.
Artigo em Inglês | MEDLINE | ID: mdl-36564340

RESUMO

Phytosterols can be used by microorganisms as carbon and energy sources and completely degraded into CO2 and H2 O. The catabolic pathway of phytosterols was well characterized in many microorganisms. Blocking the steroid core ring degradation by deletions of fadE30 and fadD3 genes, two important steroid intermediates, 3aα-H-4α-(3'-Propionic acid)-5α-hydroxy-7aß-methylhexahydro-1-indanone-δ-lactone (sitolactone, or HIL) and 3aα-H-4α-(3'-propionic acid)-7aß-methylhexahydro-1,5-indanedione (HIP) can be accumulated. They are currently used to synthesize nor-steroid drugs with an α-methyl group or without the methyl group at the C10 -position, such as estrone and norethindrone. In this study, a key gene involved in the bioconversion of HIP to HIL was identified in Mycolicibacterium neoaurum. Through heterologous expression, gene hipR was found to be involved in the reduction of the C5 keto group of HIP to a hydroxy group, leading to spontaneously lactonization into HIL in vitro. Through gene complementation and knockout, HipR functions were verified and two HIP degradation pathways in vivo were elucidated. The finding of this research facilitated the understanding of the metabolic pathway of sterols, and was directly applied to engineering robust production strains by overexpression or knockout of related genes.

19.
Sci Bull (Beijing) ; 67(15): 1562-1571, 2022 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-36546284

RESUMO

The severe bulk recombination and sluggish oxygen evolution reaction (OER) dynamics of photoanodes severely restrict the application of photoelectrochemical (PEC) devices. To solve these two problems, crystallographic facet orientation and cocatalyst emergence with a high-quality photoanode/cocatalyst interface were realized through an air annealing-assisted strategy to treat atomic layer deposition (ALD)-modified SnS2 nanosheet arrays. Based on experimental observations and theoretical calculations, the reduced (001) crystal facet of SnS2 decreases the recombination of photogenerated carriers in the bulk and improves the carrier separation of the photoanode. Moreover, the unexpectedly formed ZnTiOxSy film decreases the overpotential of the surface OER, reduces interface recombination, and extends the carrier lifetime. These synergistic effects lead to significantly enhanced PEC performance, with a high photocurrent density of 1.97 mA cm-2 at 1.23 V vs. reversible hydrogen electrode (RHE) and a low onset potential of 0.21 V vs. RHE, which are superior to reported mostly SnS2-based photoanodes.

20.
Oncogene ; 2022 Dec 14.
Artigo em Inglês | MEDLINE | ID: mdl-36517590

RESUMO

Glioblastoma (GBM) is the most lethal primary brain tumor in adults and harbors a subpopulation of glioma stem cells (GSCs). Enhancer of Zeste Homolog 2 (EZH2), a histone lysine methyltransferase, deeply involves in the stemness maintenance of GSC. However, the precise mechanism and therapeutic potential remain elusive. We postulated that the interactome of EZH2 in GSC is unique. Therefore, we performed proteomic and transcriptomic research to unveil the oncogenic mechanism of EZH2. Immunoprecipitation and mass spectrometry were used to identify proteins that co-precipitate with EZH2. We show that EZH2 binds to heterochromatin protein 1 binding protein 3 (HP1BP3) in GSCs and impairs the methylation of H3K9. Overexpression of HP1BP3 enhances the proliferation, self-renewal and temozolomide (TMZ) resistance of GBM cells. Furthermore, EZH2 and HP1BP3 co-activate WNT7B expression thereby increasing TMZ resistance and stemness of GBM cells. Importantly, inhibition of WNT7B autocrine via LGK974 effectively reverses the TMZ resistance. Our work clarifies a new oncogenic mechanism of EZH2 by which it interacts with HP1BP3 and epigenetically activates WNT7B thereby promoting TMZ resistance in GSCs. Our results provide a rationale for targeting WNT/ß-catenin pathway as a promising strategy to overcome TMZ resistance in GSCs.

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