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1.
J Nanosci Nanotechnol ; 21(9): 4705-4714, 2021 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-33691856

RESUMO

Selective photocatalysis is a very promising direction to improve the activities of photocatalysts. Combining the technique of molecular imprinting (MIP) with heterogeneous photocatalysis can be an appealing approach to achieve our aim. Herein, using the MIP technique, the monoclinic MIP-BiVO4 was successfully synthesized by the presence of rhodamine B (RhB) during the hydrothermal synthesis. The synthesized MIP-BiVO4 possessed better adsorptive and photocatalytic activities than pristine BiVO4. RhB added in the synthesis process worked as a template and served a crucial role in the formation of the MIP-BiVO4 morphology. The photoelectrochemical analysis verified the superiority of MIP-BiVO4 sample in the transfer and separation of the electron-hole pairs. Holes played the most crucial role in the degradation of the pollutants. The effective approach combining MIP technique in the synthesis of photocatalysts would provide some guidance to selective photocatalysis field for designing and synthesizing highly efficient photocatalysts.

2.
Preprint | bioRxiv | ID: ppbiorxiv-426875

RESUMO

Antiviral compounds displaying remarkable features have been identified by an unconventional drug screen and advanced through animal validation. Efficacy is observed against the six viral families causing most human respiratory viral disease, irrespective of strain, including both influenza (FLUV) and SARS-CoV-2, with cell culture EC50 at or below 100 nM. Survival benefit is demonstrated in pigs against another member of family Coronaviridae, porcine epidemic diarrhea virus (PEDV), and shown equally effective in mild and severe disease. Respiratory syncytial virus (RSV) titer is reduced by drug treatment in cotton rats. A substantial barrier to viral resistance is demonstrated for FLUV. Drug resin affinity chromatography (DRAC) reveals a novel drug target: a multi-protein complex (MPC) formed transiently, in an energy-dependent fashion, and composed of host proteins implicated in both viral lifecycles and manipulation of innate immunity. The protein composition of this host MPC is modified upon viral infection, with increase or decrease of some proteins and appearance or complete loss of others. Valosin-containing protein, also known as Transitional endoplasmatic reticulum ATPase (VCP/p97), is present in the target MPC of uninfected cells and significantly increased in both FLUV and CoV infection. SQSTM1/p62, a key regulator of the autophagy pathway of innate immunity whose dysfunction is implicated in cytokine storm, is i) found in the target MPC from uninfected cells, ii) diminished in DRAC eluates by infection, and iii) restored by drug treatment of infected cells. 14-3-3 is one of likely several proteins that comprise the drug-binding site. Advanced compounds with improved pharmacokinetic (PK) properties and lung exposure are approaching criteria for a Target Product Profile. We propose these novel drug targets to comprise a previously unappreciated molecular basis for homeostasis that is modified by viruses to allow exploitation for viral propagation and is restored by treatment with the therapeutic compounds presented. This discovery has transformative implications for treatment of respiratory viral-related disease, applicable to everything from seasonal FLUV to COVID-19 and future novel respiratory viruses, due to the pan-family nature of drug activity and barrier to resistance development.

3.
Zhongguo Zhong Yao Za Zhi ; 45(19): 4740-4745, 2020 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-33164441

RESUMO

This study is to explore the effect of Xiangdan Injection on anticoagulation of warfarin in rats. Rats were randomly divided into different groups and then administered, subsequently the blood samples were collected at a set series of time points to measure PT(prothrombin time) and APTT(activated partial thromboplastin time) values, and INR(international normalized ratio) value was calculated. The plasma concentrations of warfarin enantiomers were determined by UPLC-MS/MS technology, and pharmacokinetic parameters were calculated by DAS 2.0 software. Statistical analysis was performed to compare differences between the groups. Single-dose study of warfarin showed that Xiangdan Injection alone had no effects on PT, APTT and INR, but when co-administrated with warfarin, PT and INR values were increased(P<0.01), while APTT was unaffected; after co-administration of the two drugs, C_(max), AUC_(0-t), and AUC_(0-∞) of S-warfarin increased(P<0.01), and t_(1/2) prolonged(P<0.01), while the pharmacokinetic parameters of R-warfarin were not changed significantly. Steady-state study of warfarin showed that after co-administration of the two drugs, the PT and INR values increased(P<0.05), and the plasma concentration of S-warfarin increased(P<0.01), while the plasma concentration of R-warfarin was not changed significantly. The results suggest that Xiangdan Injection itself has no effect on coagulation index, but can enhance the anticoagulant effect of warfarin by slowing metabolism of S-warfarin.


Assuntos
Anticoagulantes , Varfarina , Animais , Cromatografia Líquida , Medicamentos de Ervas Chinesas , Tempo de Protrombina , Ratos , Espectrometria de Massas em Tandem
4.
Infect Genet Evol ; 68: 58-67, 2019 03.
Artigo em Inglês | MEDLINE | ID: mdl-30529719

RESUMO

Feline kobuvirus (FeKoV), a novel picornavirus of the genus kobuvirus, was initially identified in the feces of cats with diarrhea in South Korea in 2013. To date, there is only one report of the circulation of kobuvirus in cats in southern China. To investigate the presence and genetic variability of FeKoV in northeast China, 197 fecal samples were collected from 105 cats with obvious diarrhea and 92 asymptomatic cats in Shenyang, Jinzhou, Changchun, Jilin and Harbin regions, Northeast China, and viruses were detected by RT-PCR with universal primers targeting all kobuviruses. Kobuvirus was identified in 28 fecal samples with an overall prevalence of 14.2% (28/197) of which 20 samples were co-infected with feline parvovirus (FPV) and/or feline bocavirus (FBoV). Diarrhoeic cats had a higher kobuvirus prevalence (19.1%, 20/105) than asymptomatic cats (8.7%, 8/92). By genetic analysis based on partial 3D gene, all kobuvirus-positive samples were more closely related to previous FeKoV strains with high identities of 90.5%-97.8% and 96.6%-100% at the nucleotide and amino acid levels. Additionally, phylogenetic analysis based on the complete VP1 gene indicated that all FeKoV strains identified in this study were placed into a cluster, which separated from other reference strains previously reported, and three identical amino acid substitutions were present at the C-terminal of the VP1 protein for these FeKoV strains. Furthermore, two complete FeKoV polyprotein genomes were successfully obtained from two positive samples and designated 16JZ0605 and 17CC0811, respectively. The two strains shared 92.9%-94.9% nucleotide identities and 96.8%-98.4% amino acid identities to FeKoV prototype strains. Phylogenetic analysis indicated that FeKoVs were clustered according to their geographical regions, albeit with limited sequences support. This study provides the first molecular evidence that FeKoV circulates in cats in northeast China, and these FeKoVs exhibit genetic diversity and unique evolutionary trend.


Assuntos
Doenças do Gato/epidemiologia , Doenças do Gato/virologia , Kobuvirus/classificação , Kobuvirus/genética , Infecções por Picornaviridae/veterinária , Animais , Proteínas do Capsídeo/química , Proteínas do Capsídeo/genética , Gatos , China/epidemiologia , Genoma Viral , Genômica/métodos , Kobuvirus/isolamento & purificação , Epidemiologia Molecular , Filogenia , Análise de Sequência de DNA
5.
Int J Prosthodont ; 30(3): 238-241, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28452999

RESUMO

Two patients with a reduced vertical dimension of occlusion as a result of teeth wear were prescribed ceramic overlays. Their 2-year follow-up suggested good adaptation to the recovered muscle support without associated symptoms or adverse alterations in the restorations.


Assuntos
Desenho Assistido por Computador , Planejamento de Dentadura , Revestimento de Dentadura , Desgaste dos Dentes/reabilitação , Dimensão Vertical , Idoso , Cerâmica , Modelos Dentários , Feminino , Humanos , Pessoa de Meia-Idade
6.
Artif Organs ; 41(5): 470-475, 2017 May.
Artigo em Inglês | MEDLINE | ID: mdl-27878830

RESUMO

The objective of this study is to compare the myocardium protective effect of Bretschneider's histidine-tryptophan-ketoglutarate (HTK) solution versus Modified St. Thomas' (STH) solution in pediatric cardiac surgery of Tetralogy of Fallot (TOF). Seventy-seven pediatric patients of TOF who received the total surgical repair were reviewed, from January 2014 to October 2015. A horizontal comparison between HTK solution and modified STH solution has been made since the HTK solutions were started to be used in our hospital. The patients were divided into the HTK group (n = 35) and the STH group (n = 33). The perioperative values of the groups were assessed in this study. The primary endpoints including spontaneous cardiac re-beating time, intensive care unit (ICU) stay, overall stay, mechanical ventilation postoperation, postoperation stay, overall stay, and perioperative echocardiographic results were analyzed in this study. We found that spontaneous cardiac re-beating time of the HTK group was significantly shorter than that of the STH group (0.26 min ± 0.56 vs. 1.33 ± 1.02, P < 0.001). There were no significant differences between the two groups in ICU stay (P = 0.29), postoperative mechanical ventilation time (P = 0.84), overall stay (0.73); and the mortalities of the two groups were similar (2.9 vs. 3.0%). Aimed at pediatric cardiac surgery of TOF, this study suggests that with similar aorta cross-clamping time, modified STH solution is as safe as HTK solution.


Assuntos
Soluções Cardioplégicas/uso terapêutico , Coração/efeitos dos fármacos , Tetralogia de Fallot/cirurgia , Adolescente , Adulto , Bicarbonatos/uso terapêutico , Cloreto de Cálcio/uso terapêutico , Criança , Pré-Escolar , Feminino , Glucose/uso terapêutico , Parada Cardíaca Induzida/métodos , Humanos , Lactente , Magnésio/uso terapêutico , Masculino , Manitol/uso terapêutico , Contração Miocárdica/efeitos dos fármacos , Cloreto de Potássio/uso terapêutico , Procaína/uso terapêutico , Cloreto de Sódio/uso terapêutico , Adulto Jovem
7.
Dalton Trans ; 45(46): 18566-18571, 2016 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-27813542

RESUMO

A new copper-based coordination compound Cu2(2,2'-bipy)2(pfbz)4 (1) (where 2,2'-bipy = 2,2'-bipyridine; pfbz = pentafluorobenzoate), was hydrothermally synthesized and structurally characterized. Compound 1 having a binuclear structure consists of two copper cations and two oxygen atoms alternately in a plane square arrangement. In the presence of very small amounts of H2O2, the catalytic properties of compound 1 for the degradation of methyl orange (MO) are excellent in the absence of UV-visible radiation. Moreover, compound 1 presents suitable properties for degradation of Congo red (CR). Our results indicated that the five-coordinated copper compound, 1, will be a promising candidate for efficient degradation of organic dyes.

8.
Food Chem ; 190: 33-40, 2016 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-26212938

RESUMO

The effect of dry-curing salt content (4% low salt (LS), 8% high salt (HS)) on lipolysis, lipid oxidation and volatile compounds in dry-cured goose was investigated in our study. The activities of acid lipase and neutral lipase increased during dry-curing, while phospholipase reached its maximum at the end of marinating. Lipoxygenase (LOX) and thiobarbituric acid reactive substances (TBARS) values increased during dry-curing and marinating then decreased during dry-ripening. Total free fatty acids (TFFA) increased at dry-curing and dry-ripening points and decreased during marinating. Total peak area of lipids derived volatile compounds (TPALDVC) and total peak area increased during entire stages. Compared to LS, HS group has higher lipolytic and LOX activities, TBARS, TFFA, unsaturated fatty acids and TPALDVC. The higher TPALDVC in HS could be attributed to higher lipid hydrolysis and oxidation during processing.


Assuntos
Gansos , Lipólise/genética , Produtos da Carne/análise , Animais , Aromatizantes , Manipulação de Alimentos , Armazenamento de Alimentos , Tecnologia de Alimentos , Oxirredução , Cloreto de Sódio , Compostos Orgânicos Voláteis
9.
Strahlenther Onkol ; 191(1): 34-42, 2015 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25293728

RESUMO

BACKGROUND: Previous studies showed that noncoplanar intensity-modulated radiotherapy (NC-IMRT) for sinonasal cancer is superior to coplanar intensity-modulated radiotherapy (IMRT). Volumetric-modulated arc therapy (VMAT) is a newly introduced treatment modality, and the performance of noncoplanar VMAT for sinonasal cancer has not been well described to date. PURPOSE: To compare the dosimetry difference of noncoplanar VMAT (NC-VMAT), coplanar VMAT (co-VMAT), and NC-IMRT for sinonasal cancer. PATIENTS AND METHODS: Ten postoperative patients with sinonasal cancer were randomly selected for planning with NC-VMAT, co-VMAT, and NC-IMRT. Two planning target volumes (PTVs) were contoured representing high-risk and low-risk regions set to receive a median absorbed dose (D50%) of 68 Gy and 59 Gy, respectively. The homogeneity index (HI), conformity index (CI), dose-volume histograms (DVHs), and delivery efficiency were all evaluated. RESULTS: Both NC-VMAT and co-VMAT showed superior dose homogeneity and conformity in PTVs compared with NC-IMRT. There was no significant difference between NC-VMAT and co-VMAT in PTV coverage. Both VMAT plans provided a better protection for organs at risk (OARs) than NC-IMRT plans, and NC-VMAT showed a small improvement over co-VMAT in sparing of OARs. For peripheral doses, the doses to breast, thyroid, and larynx in the NC-IMRT plans were significantly higher than those in both VMAT plans. Compared to NC-VMAT, co-VMAT significantly reduced peripheral doses. NC-VMAT and co-VMAT reduced the average delivery time by 63.2 and 64.2%, respectively, in comparison with NC-IMRT. No differences in delivery efficiency were observed between the two VMAT plans. CONCLUSION: Compared to NC-VMAT, co-VMAT showed similar PTV coverage and comparable OAR sparing but significantly reduced peripheral doses and positioning uncertainty. We propose to give priority to coplanar VMAT in the treatment of sinonasal cancer.


Assuntos
Tratamentos com Preservação do Órgão/métodos , Órgãos em Risco/efeitos da radiação , Neoplasias dos Seios Paranasais/radioterapia , Planejamento da Radioterapia Assistida por Computador/métodos , Radioterapia de Intensidade Modulada/métodos , Absorção de Radiação , Idoso , Carga Corporal (Radioterapia) , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Dosagem Radioterapêutica , Resultado do Tratamento
10.
Zhonghua Nan Ke Xue ; 17(1): 27-31, 2011 Jan.
Artigo em Chinês | MEDLINE | ID: mdl-21351528

RESUMO

OBJECTIVE: To explore the association of spermatogenic arrest with the expression of estrogen receptor alpha (ERalpha) in human testes. METHODS: We examined the testicular biopsy specimens of 120 infertile men by HE staining, detected the expression of ERalpha in the specimens of those with spermatogenic arrest by the two-step immunohistochemical method, and compared the results with those of 10 healthy men. RESULTS: Of the 120 specimens from the infertile men, 31 (25.8%) met the diagnostic criteria of spermatogenic arrest. In the testis tissue of normal men, ERalpha expressed in Sertoli, myoid and Leydig cells, but not in spermatogenic cells, while in the testis tissues of those with spermatogenic arrest, ERalpha expressed lowly in Sertoli, myoid and Leydig cells, with statistically significant differences in immunostaining intensity between the two groups (P < 0.05). CONCLUSION: Androgen receptor (AR) and ERalpha may play a coordinating role in facilitating spermatogenesis. Spermatogenic arrest may be related to a complex series of disorders in cell signal transduction involving AR, ERalpha and HSP90.


Assuntos
Receptor alfa de Estrogênio/metabolismo , Infertilidade Masculina/metabolismo , Testículo/metabolismo , Adulto , Estudos de Casos e Controles , Humanos , Infertilidade Masculina/etiologia , Infertilidade Masculina/patologia , Masculino , Espermatogênese , Testículo/patologia , Adulto Jovem
11.
Diagn Microbiol Infect Dis ; 69(1): 7-11, 2011 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-21146708

RESUMO

We evaluated the Kanagawa hemolytic test and tdh gene test for accuracy in identifying pathogenic Vibrio parahaemolyticus isolates in Shanghai. One hundred and seventy-two V. parahaemolyticus isolates were collected from diarrhea patients, freshly harvested sea fish, or fresh water samples. Statistical data for the Kanagawa hemolytic test and tdh gene test were compared. There were 83.51% isolates (81/97) from patients and 22.22% isolates (10/45) from sea-fish positive for the tdh gene. However, none of 30 isolates from fresh water samples were tdh-positive. Positive Kanagawa hemolytic tests were obtained in 88.66%, 46.67%, and 76.67% of isolates, which were from patients, sea fish, and fresh water samples, respectively. Positive rates of the Kanagawa hemolytic tests and the tdh gene tests were significantly different in isolates from those 3 sources (P < 0.001). The tdh gene test showed higher specificity than the Kanagawa hemolytic test on identifying pathogenic V. parahaemolyticus isolates in Shanghai, China.


Assuntos
Técnicas Bacteriológicas/métodos , Peixes/microbiologia , Proteínas Hemolisinas/genética , Proteínas Hemolisinas/metabolismo , Vibrioses/diagnóstico , Vibrio parahaemolyticus/isolamento & purificação , Microbiologia da Água , Animais , Toxinas Bacterianas/genética , Toxinas Bacterianas/metabolismo , China , DNA Bacteriano/genética , Hemólise , Humanos , Sensibilidade e Especificidade , Vibrio parahaemolyticus/genética , Vibrio parahaemolyticus/patogenicidade
12.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 28(6): 577-9, 2010 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-21365826

RESUMO

Comparing of dental clinical management in the USA, the authors would like to make some suggestions to Chinese colleagues in the following five aspects. They are included disadvantages of using disposable examination kit, using chair side X-ray machine, stopping using chair side spittoon, using topical anesthesia instead of using topical disinfection, patient management in the university hospital setting. The aims of this article were to open discussion and improve Chinese dental clinical management.


Assuntos
China , Clínicas Odontológicas/organização & administração , Humanos
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