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1.
Malays J Med Sci ; 26(3): 119-128, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-31303856

RESUMO

Background: Self-efficacy (SE) is a person's belief in his or her own capability to perform and accomplish a task that could produce a favourable outcome, despite facing obstacles. This study aimed to confirm the validity and reliability of an SE scale among undergraduate students at the Health Campus of the Universiti Sains Malaysia. Methods: A cross-sectional study was conducted among the undergraduate students using a self-administered questionnaire. After using a purposive sampling method, 562 students completed the questionnaire. Mplus 8 was employed to conduct the confirmatory factor analysis on the psychometric properties of Bandura's 18-item SE scale with three factors (internal feeling, competing demands and situational). Then, the composite reliability was calculated for each factor. Results: Most of the students were Malay (73.3%) females (79.0%) who exercised 2.62 times a week for an average of 43.37 min per session. The final measurement model was obtained after removing six problematic items, and the model was deemed fit based on several indices [Root Mean Square Error of Approximation (RMSEA) = 0.067, Standardised Root Mean Square Residual (SRMR) = 0.004, Comparative Fit Index (CFI) = 0.924]. The composite reliability values of the three factors were acceptable (0.65 to 0.84). Conclusion: The simplified 12-item SE scale with three factors displayed good fit indices with regard to the data, and they were considered to be acceptable for the current sample.

2.
Front Neurol ; 9: 412, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-29962999

RESUMO

Alzheimer's disease (AD), a progressive neurodegenerative disorder, lacks preclinical diagnostic biomarkers and therapeutic drugs. Thus, earlier intervention in AD is a top priority. Studies have shown that the gut microbiota influences central nervous system disorders and that prebiotics can improve the cognition of hosts with AD, but these effects are not well understood. Preliminary research has shown that oligosaccharides from Morinda officinalis (OMO) are a useful prebiotic and cause substantial memory improvements in animal models of AD; however, the mechanism is still unclear. Therefore, this study was conducted to investigate whether OMO are clinically effective in alleviating AD by improving gut microbiota. OMO were administered to APP/PS1 transgenic mice, and potential clinical biomarkers of AD were identified with metabolomics and bioinformatics. Behavioral experiments demonstrated that OMO significantly ameliorated the memory of the AD animal model. Histological changes indicated that OMO ameliorated brain tissue swelling and neuronal apoptosis and downregulated the expression of the intracellular AD marker Aß1-42. 16S rRNA sequencing analyses indicated that OMO maintained the diversity and stability of the microbial community. The data also indicated that OMO are an efficacious prebiotic in an animal model of AD, regulating the composition and metabolism of the gut microbiota. A serum metabolomics assay was performed using UHPLC-LTQ Orbitrap mass spectrometry to delineate the metabolic changes and potential early biomarkers in APP/PS1 transgenic mice. Multivariate statistical analysis showed that 14 metabolites were significantly upregulated, and 8 metabolites were downregulated in the model animals compared to the normal controls. Thus, key metabolites represent early indicators of the development of AD. Overall, we report a drug and signaling pathway with therapeutic potential, including proteins associated with cognitive deficits in normal mice or gene mutations that cause AD.

3.
Neuroimage ; 175: 100-110, 2018 07 15.
Artigo em Inglês | MEDLINE | ID: mdl-29621615

RESUMO

The field of cognitive neuroscience is weighing evidence about whether to move from standard field strength to ultra-high field (UHF). The present study contributes to the evidence by comparing a cognitive neuroscience paradigm at 3 Tesla (3T) and 7 Tesla (7T). The goal was to test and demonstrate the practical effects of field strength on a standard GO/NOGO task using accessible preprocessing and analysis tools. Two independent matched healthy samples (N = 31 each) were analyzed at 3T and 7T. Results show gains at 7T in statistical strength, the detection of smaller effects and group-level power. With an increased availability of UHF scanners, these gains may be exploited by cognitive neuroscientists and other neuroimaging researchers to develop more efficient or comprehensive experimental designs and, given the same sample size, achieve greater statistical power at 7T.


Assuntos
Encéfalo/fisiologia , Interpretação Estatística de Dados , Função Executiva/fisiologia , Neuroimagem Funcional/métodos , Imagem por Ressonância Magnética/métodos , Adulto , Encéfalo/diagnóstico por imagem , Neuroimagem Funcional/normas , Humanos , Imagem por Ressonância Magnética/normas
4.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 31(3): 291-293, 2018 Jun 01.
Artigo em Chinês | MEDLINE | ID: mdl-31544409

RESUMO

OBJECTIVE: To investigate the species and activities of wild animals infected with schistosome in hilly and mountain areas by using an infrared camera technique, so as to provide the evidence for the surveillance of schistosomiasis in these areas. METHODS: Six infrared cameras were selected and placed in 6 environments of the risk monitoring points of schistosomiasis in Shitai County in Anhui Province. The species and activities of the wild animals in the 6 environments were observed through the photographs and videos taken by the cameras. RESULTS: Through 5 day's monitoring, 3 wild mammals, such as voles, hares and wild boars, were found in 4 monitoring environments, of which voles were found at 2 environments with snails, and hares, wild boars and voles were found in 2 environments adjacent to environments with snails respectively. The monitoring showed that the vole activity was most frequent in the monitored environment. CONCLUSIONS: The use of infrared camera technique has a good effect in the investigation of wild animal infectious source of schistosomiasis, and it is also suitable for the monitoring work in other types of environments.


Assuntos
Distribuição Animal , Animais Selvagens , Raios Infravermelhos , Mamíferos , Fotografação , Esquistossomose , Animais , Animais Selvagens/parasitologia , China , Reservatórios de Doenças , Mamíferos/parasitologia , Mamíferos/fisiologia , Schistosoma , Esquistossomose/transmissão , Caramujos
5.
Zhongguo Xue Xi Chong Bing Fang Zhi Za Zhi ; 28(4): 422-425, 2016 Jun 22.
Artigo em Chinês | MEDLINE | ID: mdl-29376285

RESUMO

OBJECTIVE: To characterize the population dynamics and spatial distribution of Aleuroglyphus ovatus in the flour warehouse, so as to provide the basic evidence for improving the sampling guidelines that are essential for effective pest monitoring and management. METHODS: The samples from flour warehouses of four localities were collected, examined and counted for A. ovatus in every month in Wuhu City. The dispersion pattern target, Iwao m*/x̅ regression analysis and Taylor power method were used for analyzing the spatial distribution pattern of A. ovatus in the flour warehouses. RESULTS: The peaks of population dynamics of A. ovatus were discovered in July and September, respectively. The indexes of dispersion were as follows:I > 0, CA > 0, m*/x̅ > 1. At the same time, the parameters in the equation of Iwao:m*=5.471+1.022 x̅ (r = 0.999) and Taylor: lgS2 = 0.697+1.111 lg x̅ (r = 0.987) showed that the spatial distribution pattern of A. ovatus was assembled. CONCLUSIONS: The peaks of population dynamics of A. ovatus in the flour warehouse are bimodal pattern, and the spatial distribution pattern of A. ovatus is assembled.


Assuntos
Acaridae , Distribuição Animal , Animais , Farinha/parasitologia , Modelos Lineares , Dinâmica Populacional , Estações do Ano , Análise Espacial
6.
Front Psychol ; 6: 1506, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26528200

RESUMO

Behavioral inhibitory control has been shown to play an important role in a variety of addictive behaviors. A number of studies involving the use of Go/NoGo and stop-signal paradigms have shown that smokers have reduced response inhibition for cigarette-related cues. However, it is not known whether male light smokers' response inhibition for cigarette-related cues is lower than that of non-smokers in the two-choice oddball paradigm. The objective of the current study was to provide further behavioral evidence of male light smokers' impaired response inhibition for cigarette-related cues, using the two-choice oddball paradigm. Sixty-two male students (31 smokers, 31 non-smokers), who were recruited via an advertisement, took part in this two-choice oddball experiment. Cigarette-related pictures (deviant stimuli) and pictures unrelated to cigarettes (standard stimuli) were used. Response inhibition for cigarette-related cues was measured by comparing accuracy (ACC) and reaction time (RT) for deviant and standard stimuli in the two groups of subjects. An analysis of variance (ANOVA) showed that in all the participants, ACC was significantly lower for deviant stimuli than for standard stimuli. For deviant stimuli, the RTs were significantly longer for male light smokers than for male non-smokers; however, there was no significant difference in RTs for standard stimuli. Compared to male non-smokers, male light smokers seem to have a reduced ability to inhibit responses to cigarette-related cues.

7.
Sci Rep ; 5: 16582, 2015 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-26560971

RESUMO

Systematic reviews (SRs) of randomized controlled trials (RCTs) have demonstrated acupuncture's effectiveness in stroke rehabilitation. The current study reviews the quality of evidence in SRs of acupuncture in stroke rehabilitation, and rates the strength of recommendation for its use based on this evidence using the GRADE (grading of recommendations, assessment, development and evaluations) approach. A comprehensive literature search was performed using multiple databases (e.g., Medline, Embase) with advanced search strategies. Two authors independently selected articles, collected data, and assessed the methodological quality of each identified SR according to AMSTAR (a measurement tool to assess systematic reviews) and OQAQ (Oxman and Guyatt's overview quality assessment questionnaire). Outcomes related to stroke rehabilitation were evaluated. SRs of high methodological quality (AMSTAR score ≥9 and OQAQ score ≥7) were graded using GRADE. Ultimately, acupuncture yields benefits in stroke rehabilitation (neurological function improvement: RR = 1.34; swallowing improvement: RR = 1.61, 1.49, 1.07; disability: SMD = 0.49 or 0.07). Poor evidentiary quality and insufficient information about harm led to weak recommendations. In conclusion, acupuncture may improve stroke rehabilitation, as the GRADE approach indicated a weak recommendation for acupuncture's usage in this context.


Assuntos
Terapia por Acupuntura , Reabilitação do Acidente Vascular Cerebral , Acidente Vascular Cerebral/terapia , Humanos , Razão de Chances , Viés de Publicação , Ensaios Clínicos Controlados Aleatórios como Assunto , Reprodutibilidade dos Testes , Resultado do Tratamento
8.
IEEE Trans Image Process ; 24(4): 1424-35, 2015 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-25700445

RESUMO

In this paper, we propose an approach to learn hierarchical features for visual object tracking. First, we offline learn features robust to diverse motion patterns from auxiliary video sequences. The hierarchical features are learned via a two-layer convolutional neural network. Embedding the temporal slowness constraint in the stacked architecture makes the learned features robust to complicated motion transformations, which is important for visual object tracking. Then, given a target video sequence, we propose a domain adaptation module to online adapt the pre-learned features according to the specific target object. The adaptation is conducted in both layers of the deep feature learning module so as to include appearance information of the specific target object. As a result, the learned hierarchical features can be robust to both complicated motion transformations and appearance changes of target objects. We integrate our feature learning algorithm into three tracking methods. Experimental results demonstrate that significant improvement can be achieved using our learned hierarchical features, especially on video sequences with complicated motion transformations.

9.
Asian Pac J Cancer Prev ; 15(7): 3331-3, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-24815492

RESUMO

BACKGROUND: The results of previous researches that analyzed the association between genetic polymorphisms of transcription factor-7-like 2 (TCF7L2, rs7903146) and polycystic ovary syndrome (PCOS) were conflicting. Current systematic analysis was conducted to re-explore this association using updated materials. MATERIALS AND METHODS: The PubMed database was used for data collection and the final search was conducted on January 3, 2014. For TCF7L2 rs7903146, a non-significant slight increase in risk of PCOS development was observed under three genetic models (dominant model: OR=1.06, 95%CI: 0.93-1.21, p>0.05; recessive model: OR=1.12, 95%CI: 0.87-1.43, p> 0.05; homozygous model: OR=1.14, 95%CI: 0.87-1.47, p>0.05). In the subgroup analyses in Asian group, allele susceptibility of PCOS was calculated (allele model: OR=1.00, 95%CI: 0.74-1.35, p>0.05; dominant model: OR=0.98, 95%CI: 0.71-1.35, p>0.05; recessive model: OR=1.79, 95%CI: 0.33-9.84, p>0.05; homozygous model: OR=1.78, 95%CI: 0.32-9.80, p>0.05), the differences were again not statistically significant. CONCLUSIONS: The findings of this systemic analysis suggest that the polymorphism of TCF7L2 rs7903146 may not be associated with the susceptibility to PCOS.


Assuntos
Síndrome do Ovário Policístico/epidemiologia , Síndrome do Ovário Policístico/genética , Proteína 2 Semelhante ao Fator 7 de Transcrição/genética , Diabetes Mellitus Tipo 2/genética , Feminino , Frequência do Gene , Predisposição Genética para Doença , Humanos , Polimorfismo de Nucleotídeo Único
10.
Pathol Oncol Res ; 20(2): 309-17, 2014 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-24085601

RESUMO

Accumulating evidence indicates that the interaction of human LSCs (leukemic stem cells) with the hematopoietic microenvironment, mediated by the thrombopoietin (TPO)/c-MPL pathway, may be an underlying mechanism for resistance to cell cycle-dependent cytotoxic chemotherapy. However, the role of TPO/c-MPL signaling in AML (acute myelogenous leukemia) chemotherapy resistance hasn't been fully understood. The c-MPL and TPO levels in different AML samples were measured by flow cytometry and ELISA. We also assessed the TPO levels in the osteoblasts derived from bone mesenchymal stem cells (BMSCs). The survival rate of an AML cell line that had been co-cultured with different BMSC-derived osteoblasts was measured to determine the IC50 of an AML chemotherapy drug daunorubicin (DNR). The levels of TPO/c-MPL in the initial and relapse AML patients were significantly higher than that in the control (P < 0.05). The osteoblasts derived from AML patients' BMSCs secreted more TPO than the osteoblasts derived from normal control BMSCs (P < 0.05). A strong positive correlation between the TPO level and c-MPL expression was found in the bone marrow mononuclear cells of the relapse AML patients. More importantly, the IC50 of DNR in the HEL + AML-derived osteoblasts was the highest among all co-culture systems. High level of TPO/c-MPL signaling may protect LSCs from chemotherapy in AML. The effects of inhibition of the TPO/c-MPL pathway on enhancing the chemotherapy sensitivity of AML cells, and on their downstream effector molecules that direct the interactions between patient-derived blasts and leukemia repopulating cells need to be further studied.


Assuntos
Autoantígenos/metabolismo , Medula Óssea/metabolismo , Iodeto Peroxidase/metabolismo , Proteínas de Ligação ao Ferro/metabolismo , Leucemia Mieloide Aguda/metabolismo , Leucemia Mieloide Aguda/mortalidade , Receptores de Trombopoetina/metabolismo , Adolescente , Adulto , Idoso , Estudos de Casos e Controles , Ciclo Celular/fisiologia , Linhagem Celular , Criança , Técnicas de Cocultura , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Osteoblastos/metabolismo , Transdução de Sinais/fisiologia , Células-Tronco/metabolismo , Taxa de Sobrevida , Adulto Jovem
11.
Braz. j. med. biol. res ; 45(10): 906-912, Oct. 2012. ilus, tab
Artigo em Inglês | LILACS | ID: lil-647750

RESUMO

Hypoxia inducible factor-1α (HIF-1α) is an important transcription factor, which plays a critical role in the formation of solid tumor and its microenviroment. The objective of the present study was to evaluate the expression and function of HIF-1α in human leukemia bone marrow stromal cells (BMSCs) and to identify the downstream targets of HIF-1α. HIF-1α expression was detected at both the RNA and protein levels using real-time PCR and immunohistochemistry, respectively. Vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1α (SDF-1α) were detected in stromal cells by enzyme-linked immunosorbent assay. HIF-1α was blocked by constructing the lentiviral RNAi vector system and infecting the BMSCs. The Jurkat cell/BMSC co-cultured system was constructed by putting the two cells into the same suitable cultured media and conditions. Cell adhesion and secretion functions of stromal cells were evaluated after transfection with the lentiviral RNAi vector of HIF-1α. Increased HIF-1α mRNA and protein was detected in the nucleus of the acute myeloblastic and acute lymphoblastic leukemia compared with normal BMSCs. The lentiviral RANi vector for HIF-1α was successfully constructed and was applied to block the expression of HIF-1α. When HIF-1α of BMSCs was blocked, the expression of VEGF and SDF-1 secreted by stromal cells were decreased. When HIF-1α was blocked, the co-cultured Jurkat cell’s adhesion and migration functions were also decreased. Taken together, these results suggest that HIF-1α acts as an important transcription factor and can significantly affect the secretion and adhesion functions of leukemia BMSCs.


Assuntos
Humanos , Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Leucemia de Células T/metabolismo , Células-Tronco Mesenquimais/metabolismo , Adesão Celular , Ensaio de Imunoadsorção Enzimática , Imuno-Histoquímica , Células Jurkat , Reação em Cadeia da Polimerase em Tempo Real
12.
Braz J Med Biol Res ; 45(10): 906-12, 2012 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-22948410

RESUMO

Hypoxia inducible factor-1α (HIF-1α) is an important transcription factor, which plays a critical role in the formation of solid tumor and its microenvironment. The objective of the present study was to evaluate the expression and function of HIF-1α in human leukemia bone marrow stromal cells (BMSCs) and to identify the downstream targets of HIF-1α. HIF-1α expression was detected at both the RNA and protein levels using real-time PCR and immunohistochemistry, respectively. Vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1α (SDF-1α) were detected in stromal cells by enzyme-linked immunosorbent assay. HIF-1α was blocked by constructing the lentiviral RNAi vector system and infecting the BMSCs. The Jurkat cell/BMSC co-cultured system was constructed by putting the two cells into the same suitable cultured media and conditions. Cell adhesion and secretion functions of stromal cells were evaluated after transfection with the lentiviral RNAi vector of HIF-1α. Increased HIF-1α mRNA and protein was detected in the nucleus of the acute myeloblastic and acute lymphoblastic leukemia compared with normal BMSCs. The lentiviral RANi vector for HIF-1α was successfully constructed and was applied to block the expression of HIF-1α. When HIF-1α of BMSCs was blocked, the expression of VEGF and SDF-1 secreted by stromal cells were decreased. When HIF-1α was blocked, the co-cultured Jurkat cell's adhesion and migration functions were also decreased. Taken together, these results suggest that HIF-1α acts as an important transcription factor and can significantly affect the secretion and adhesion functions of leukemia BMSCs.


Assuntos
Subunidade alfa do Fator 1 Induzível por Hipóxia/metabolismo , Leucemia de Células T/metabolismo , Células-Tronco Mesenquimais/metabolismo , Adesão Celular , Ensaio de Imunoadsorção Enzimática , Humanos , Imuno-Histoquímica , Células Jurkat , Reação em Cadeia da Polimerase em Tempo Real
13.
Gene ; 484(1-2): 35-41, 2011 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-21664260

RESUMO

Host-pathogen interactions are complex processes, and revealing these interactions is challenging. Beauveria bassiana is a destructive pathogen to the economically beneficial silkworm, Bombyx mori, but is also a good pathogenic material for investigating insect responses to fungal infection. For better understanding of the molecular regulation of immune response and the interactive mechanism between the silkworm and B. bassiana, suppression subtractive hybridization was employed to identify differentially expressed genes in the pathogen-stimulated silkworm larvae. Complementary DNA libraries were constructed, in which 240 clones were sequenced. A total of 77 genes were found to be involved in the infection process, among which 55 were known genes and 22 were novel. Expression profiling of 6 genes by quantitative PCR showed that they were induced by fungal challenge. This study establishes the first step to understanding the molecular mechanisms by which silkworm responds to fungal infection.


Assuntos
Bombyx/genética , Bombyx/microbiologia , Expressão Gênica , Interações Hospedeiro-Patógeno , Animais , Beauveria , Perfilação da Expressão Gênica , Regulação para Cima
14.
Exp Mol Pathol ; 91(1): 461-5, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21596034

RESUMO

OBJECTIVE: To detect and compare the expression levels of two dendritic cell proteins, namely Dendritic Cell Lysosome-Associated Membrane Protein (DC-LAMP) and Dendritic Cell-Specific ICAM-3 Grabbing Non-integrin (DC-SIGN) in psoriasis vulgaris lesions and normal skin tissues. METHODS: We used Reverse Transcription-Polymerase Chain Reaction (RT-PCR) and immunohistochemical analysis to detect the expression of DC-LAMP and DC-SIGN in 33 samples representing plaque lesions of psoriasis vulgaris and 11 normal skin samples from healthy volunteers. RESULTS: RT-PCR The expression levels of DC-LAMP and DC-SIGN were higher in samples of psoriasis vulgaris lesions than in that of normal skin, based on RT-PCR results.Whereas DC-LAMP staining was mostly cytoplasmic in keratinocytes of basal and spinous epidermis and dermal dendritic cells, DC-SIGN staining was both cytoplasmic and nuclear in keratinocytes of spinous epidermis and dermal dendritic cells. The expression levels of DC-LAMP and DC-SIGN proteins were significantly higher in psoriasis vulgaris lesions when compared with normal skin tissues (P<0.01). Furthermore, we found a positive correlation between high DC-LAMP and high DC-SIGN expression levels in psoriasis vulgaris lesions (P<0.05, r=0.368). CONCLUSION: Compared with normal tissues, the levels of DC-LAMP and DC-SIGN were higher in psoriasis vulgaris lesions. Both DC-LAMP and DC-SIGN proteins may be involved in the pathogenesis of psoriasis vulgaris. The high expression of DC-LAMP may be associated with the altered differentiation of keratynocytes in psoriasis.


Assuntos
Moléculas de Adesão Celular/genética , Moléculas de Adesão Celular/metabolismo , Lectinas Tipo C/genética , Lectinas Tipo C/metabolismo , Glicoproteínas de Membrana Associadas ao Lisossomo/genética , Glicoproteínas de Membrana Associadas ao Lisossomo/metabolismo , Psoríase/genética , Psoríase/metabolismo , Receptores de Superfície Celular/genética , Receptores de Superfície Celular/metabolismo , Adolescente , Adulto , Idoso , Feminino , Expressão Gênica , Humanos , Queratinócitos/metabolismo , Queratinócitos/patologia , Masculino , Pessoa de Meia-Idade , Psoríase/patologia , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Pele/metabolismo , Pele/patologia , Adulto Jovem
15.
Eur Heart J ; 32(15): 1935-45, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-21138940

RESUMO

AIMS: Chronic kidney disease is directly associated with cardiovascular complications. Heart remodelling, including fibrosis, hypertrophy, and decreased vascularization, is frequently present in renal diseases. Our objective was to investigate the impact of calcineurin inhibitors (CNI) on cardiac remodelling and function in a rat model of renal disease. METHODS AND RESULTS: Male Sprague Dawley rats were divided into six groups: sham-operated rats, 5/6 nephrectomized rats (Nx) treated with vehicle, CNI (cyclosporine A 5.0 or 7.5, or tacrolimus 0.5 mg/kg/day) or hydralazine (20 mg/kg twice a day) for 14 days, starting on the day of surgery. Creatinine clearance was significantly lower and blood pressure significantly higher in Nx rats when compared with controls. Morphological and echocardiographic analyses revealed increased left ventricular hypertrophy and decreased number of capillaries in Nx rats. Treatment with CNI affected neither the renal function nor the blood pressure, but prevented the development of cardiac hypertrophy and improved vascularization. In addition, regional blood volume improved as confirmed by contrast agent-based echocardiography. Hydralazine treatment did not avoid heart remodelling in this model. Gene expression analysis verified a decrease in hypertrophic genes in the heart of CNI-treated rats, while pro-angiogenic and stem cell-related genes were upregulated. Moreover, mobilization of stem/progenitor cells was increased through manipulation of the CD26/SDF-1 system. CONCLUSION: We conclude from our studies that CNI-treatment significantly prevented cardiac remodelling and improved heart function in Nx rats without affecting renal function and blood pressure. This sheds new light on possible therapeutic strategies for renal patients at high cardiovascular risk.


Assuntos
Inibidores de Calcineurina , Ciclosporina/uso terapêutico , Cardiopatias/prevenção & controle , Imunossupressores/uso terapêutico , Nefropatias/complicações , Tacrolimo/uso terapêutico , Animais , Doença Crônica , Hipertrofia Ventricular Esquerda/prevenção & controle , Masculino , Nefrectomia , Distribuição Aleatória , Ratos , Ratos Sprague-Dawley , Remodelação Ventricular/efeitos dos fármacos
16.
DNA Repair (Amst) ; 9(12): 1241-8, 2010 Dec 10.
Artigo em Inglês | MEDLINE | ID: mdl-20971043

RESUMO

Maintenance of genome stability depends on efficient and accurate repair of DNA lesions. Failure to properly repair damaged DNA can cause cell death, mutations and chromosomal instability, which eventually lead to tumorigenesis. The E3 ligase RAD18 is well-known for its function in DNA damage bypass and post-replication repair (PRR) in yeast and vertebrates via its ability to facilitate PCNA mono-ubiquitination at stalled replication forks. However, emerging evidence has also indicated that RAD18 plays an important role in homologous recombination (HR) in mammalian cells, which is an error-free DNA repair pathway that mediates the repair of double-strand breaks (DSBs). Here, we review how RAD18 carries out these distinct functions in response to different types of DNA lesions.


Assuntos
Quebras de DNA de Cadeia Dupla , Reparo do DNA/fisiologia , Proteínas de Ligação a DNA/metabolismo , Instabilidade Genômica/genética , Recombinação Genética/fisiologia , Ubiquitina-Proteína Ligases/metabolismo , Reparo do DNA/genética , Genes cdc/fisiologia , Antígeno Nuclear de Célula em Proliferação/metabolismo , Ubiquitinação
17.
Braz. j. microbiol ; 39(4): 701-707, Dec. 2008. ilus, tab
Artigo em Inglês | LILACS | ID: lil-504310

RESUMO

Actinomyces strain A01 was isolated from soil of a vegetable field in the suburb of Beijing, China. According to the morphological, cultural, physiological and biochemical characteristics, and 16S rDNA sequence analysis, strain A01 was identified as Streptomyces lydicus. In the antimicrobial spectrum test strain A01 presented a stable and strong inhibitory activity against several plant pathogenic fungi such as Fusarium oxysporum, Botrytis cinerea, Monilinia laxa, etc. However, no antibacterial activity was found. In pot experiments in greenhouse, the development of tomato gray mold was markedly suppressed by treatment with the fermentation broth of the strain A01, and the control efficacy was higher than those of Pyrimethanil and Polyoxin. A main antifungal compound (purity 99.503 percent) was obtained from the fermentation broth of strain A01 using column chromatography and HPLC. The chemical structural analysis with UV, IR, MS, and NMR confirmed that the compound produced by the strain A01 is natamycin, a polyene antibiotic produced by S. chattanovgensis, S. natalensis, and S. gilvosporeus, widely used as a natural biological preservative for food according to previous reports. The present study revealed a new producing strain of natamycin and its potential application as a biological control agent for fungal plant diseases.


A cepa Actinomyces A01 foi isolada do solo de um campo agrícola no subúrbio de Beijing, China. De acordo com as características morfológicas, culturais, fisiológicas e bioquímicas, e análise da sequência 16S rDNA , a cepa A01 foi identificada como Streptomyces lydicus. Nos testes de espectro antimicrobiano, a cepa A01 apresentou atividade inibitória intensa e estável contra vários fungos patogênicos para plantas, como Fusarium oxysporum, Botrytis cinerea, Monilia laxa, etc. Entretanto, não foi encontrada atividade antibacteriana. Em experimentos em estufas, o desenvolvimento do fungo cinza do tomate foi fortemente inibido pelo tratamento com o caldo de fermentação da cepa A01, com eficiência superior à do pyremethanil e polyoxin. Por cromatografia em coluna e HPLC, obteve-se um composto fúngico (pureza 99,503 por cento), cuja análise estrutural por UV, IR, MS e NMR revelou ser natamicina, um antibiótico polienico produzido por S. chattanovgensis, S. natalensis e S.gilvosporeus, empregado como conservador biológico natural em alimentos. O presente estudo relata a detecção de uma nova cepa produtora de natamicina e sua aplicação potencial como um agente de controle biológico de doenças fúngicas em plantas.


Assuntos
Actinomyces/isolamento & purificação , Antifúngicos , Sequência de Bases , Fermentação , Fungos Mitospóricos/isolamento & purificação , Micoses , Controle Biológico de Vetores , Doenças das Plantas , Cromatografia Líquida de Alta Pressão/métodos , Métodos , Plantas , Solo , Técnicas
18.
Regul Toxicol Pharmacol ; 46(3): 211-7, 2006 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-17010489

RESUMO

Polybutylcyanoacrylate nanoparticles (PBCA-NP) were prepared by addition of the monomer to an aqueous phase containing dextran 70 and loaded with mitomycin C (MMC-PBCA-NP), a highly effective anticancer drug. When injected into mice, MMC-PBCA-NP accumulated more in the liver than did free MMC. In contrast, MMC-PBCA-NP accumulated less in the heart and kidney than did free MMC. However, when MMC-PBCA-NP and MMC were administered to rabbits bearing VX2 cells implanted into the liver, the antiproliferative effects on the tumor cells of both drugs were similar. Histological analyses indicated that organization of myocardial filaments was disrupted and vacuolization was observed in the MMC treated group, whereas MMC-PBCA-NP treatment did not appear to damage the host's heart. Hydropic degeneration of renal tubular epithelia was observed in the MMC treated group and not in the control group, whereas MMC-PBCA-NP treatment did not appear to damage the host's kidney.


Assuntos
Antibióticos Antineoplásicos/administração & dosagem , Portadores de Fármacos/administração & dosagem , Embucrilato/administração & dosagem , Neoplasias Hepáticas/tratamento farmacológico , Mitomicina/administração & dosagem , Nanopartículas , Animais , Antibióticos Antineoplásicos/farmacocinética , Linhagem Celular Tumoral , Portadores de Fármacos/farmacocinética , Embucrilato/farmacocinética , Feminino , Coração/efeitos dos fármacos , Injeções Intravenosas , Rim/efeitos dos fármacos , Rim/metabolismo , Fígado/efeitos dos fármacos , Fígado/metabolismo , Neoplasias Hepáticas/metabolismo , Camundongos , Mitomicina/farmacocinética , Miocárdio/metabolismo , Coelhos , Baço/efeitos dos fármacos , Distribuição Tecidual
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