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1.
Dent Mater J ; 2021 Oct 01.
Artigo em Inglês | MEDLINE | ID: mdl-34602588

RESUMO

This study aimed to evaluate the pulpal responses of monkey's pulp after direct pulp capping (DPC) with the novel mineral trioxide aggregate containing phosphorylated pullulan-based material (MTAPPL). Seventy-two teeth were randomly divided into four groups: MTAPPL; Nex-Cem MTA (NX); TheraCal LC (TH); and Dycal (DY). Histopathological changes in the pulps were observed at days 3, 7 and 70. On day 3, mild inflammatory responses were observed in the MTAPPL, no to moderate inflammatory responses in the TH, whereas moderate inflammatory responses in the NX and DY. No mineralized tissue formation (MTF) was observed in all groups. On day 7, no or mild inflammatory responses were observed in all groups. Initial MTF was observed except for DY. No inflammation with complete MTF including presence of odontoblast-like cells was observed in the MTAPPL, NX and TH groups at day 70. These findings indicate that MTAPPL could be an efficient DPC material.

2.
Int Endod J ; 54(10): 1902-1914, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34096634

RESUMO

AIM: To evaluate the dental pulp response to a novel mineral trioxide aggregate containing phosphorylated pullulan (MTAPPL) in rats after direct pulp capping. METHODS: Ninety-six cavities were prepared in the maxillary first molars of 56 male Wistar rats. The dental pulps were intentionally exposed and randomly divided into four groups according to the application of pulp capping materials: MTAPPL; phosphorylated pullulan (PPL); a conventional MTA (Nex-Cem MTA, NCMTA; positive control); and Super-Bond (SB; negative control). All cavities were restored with SB and observed for pulpal responses at 1-, 3-, 7- and 28-day intervals using a histological scoring system. Statistical analysis was performed using Kruskal-Wallis and Mann-Whitney U-test with Bonferroni's correction, and the level of significance was set at 0.05. DMP1 and CD34 antigen were used to evaluate odontoblast differentiation and pulpal vascularization, respectively. RESULTS: On day 1, mild inflammatory cells were present in MTAPPL and NCMTA groups; fewer inflammatory cells were present in the PPL, whereas SB was associated with a mild-to-moderate inflammatory response. A significant difference was observed between PPL and SB (p < .05). No mineralized tissue deposition was observed. On day 3, moderate-to-severe inflammatory cells were present in PPL and SB, whereas MTAPPL and NCMTA had a mild inflammatory response. Initial mineralized tissue deposition was observed in the NCMTA, MTAPPL and SB. A significant difference was observed between MTAPPL and PPL (p < .05). On day 7, a thin layer of mineralized tissue was observed in all tested groups with no or mild inflammatory response. On day 28, no inflammatory response was observed in MTAPPL, whereas NCMTA, PPL and SB had mild inflammatory responses. A significant difference was observed between MTAPPL and SB (p < .05). Complete mineralized tissue barrier formation was observed in MTAPPL, NCMTA and PPL with no significant difference (p > .05). SB exhibited incomplete mineralized tissue barriers, significantly different from NCMTA, MTAPPL and PPL (p < .05). The staining with CD34 was positive in all the groups on all observation days. CONCLUSION: The favourable pulpal responses and induction of mineralized tissue formation associated with MTAPPL indicate its potential application as a direct pulp capping material.


Assuntos
Capeamento da Polpa Dentária , Agentes de Capeamento da Polpa Dentária e Pulpectomia , Compostos de Alumínio , Animais , Compostos de Cálcio , Polpa Dentária , Combinação de Medicamentos , Glucanos , Masculino , Dente Molar , Óxidos , Agentes de Capeamento da Polpa Dentária e Pulpectomia/uso terapêutico , Ratos , Ratos Wistar , Silicatos
3.
Dent Mater ; 37(1): 106-112, 2021 01.
Artigo em Inglês | MEDLINE | ID: mdl-33229039

RESUMO

OBJECTIVE: The purpose of this study was to determine the bond stability and the change in interfacial ultra-structure of a conventional glass-ionomer cement bonded to dentin, with and without pre-treatment using a polyalkenoic acid conditioner. METHODS: The occlusal dentin surfaces of six teeth were ground flat. Glass-ionomer cement was bonded to the surfaces either with or without polyalkenoic acid conditioning. The teeth were sectioned into 1-mm2 stick-shaped specimens. The specimens obtained were randomly assigned to two groups with different periods of storage in water: 1 week and 1 year. The micro-tensile bond strength (µTBS) was determined for each storage time. Additional specimens were prepared for Transmission Electron Microscopy (TEM); they were produced with or without prior polyalkenoic acid conditioning in the same way as in the µTBS test. RESULTS: There was no significant difference in µTBS to conditioned dentin and non-conditioned dentin (p > 0.05). The failures appeared to be of a mixed nature, although aging caused more areas of cohesive than adhesive failure in both groups. The TEM observation showed an intermediate layer, a matrix-rich layer and a partially demineralized layer in the polyalkenoic acid conditioned group. SIGNIFICANCE: Aging did not reduce the bond strength of the conventional glass-ionomer cement to dentin with or without the use of a polyalkenoic acid conditioner.


Assuntos
Colagem Dentária , Cimentos de Ionômeros de Vidro , Resinas Acrílicas , Cimentos Dentários , Dentina , Adesivos Dentinários , Teste de Materiais , Microscopia Eletrônica de Varredura , Resistência à Tração
4.
J Esthet Restor Dent ; 33(6): 874-883, 2021 09.
Artigo em Inglês | MEDLINE | ID: mdl-33188588

RESUMO

OBJECTIVE: To evaluate the shade matching ability of a novel supra-nano filled esthetic resin composite employing structural color technology using simplified simulated clinical cavities. Filler morphology and light transmittance characteristics were also evaluated. MATERIALS AND METHODS: One-hundred and twenty frames of resin composite were built in A1, A2, A3, and A4 shades to simulate Class I cavities (diameter = 4 mm, height = 2 mm). For each shaded frame, cavities were filled with three different types of filler containing resin composites (n = 10): supra-nano filled (SN filled) resin composite, microhybrid filled (MH filled) resin composite, and clustered-nano filled (CN filled) resin composite. Color parameters were calculated using CIELAB (△Eab ) and CIEDE2000 (△E00 ). Data were analyzed using one-way analysis of variance (ANOVA), followed by Duncan's test (α = .05). Filler morphology and light transmittance characteristics were measured to explore the role of structural color on shade matching. RESULTS: △Eab and △E00 of SN filled resin composite were significantly lower in A2, A3, and A4 shades (P < .05). CONCLUSIONS: The SN filled resin composite showed better shade matching with A2, A3, and A4 shades of resin composite frames compared to MH filled resin composite, and CN filled resin composite. CLINICAL SIGNIFICANCE: Universal-shade resin composites, which were expected to match nearly all shades, simplify the restorative procedure. Resin composite, which contained spherical supra-nano filler particles, could contribute most to its shade matching by stimulating structural color. Structural color technology may provide additional benefits for shade matching of resin composites.


Assuntos
Cárie Dentária , Estética Dentária , Cor , Resinas Compostas , Humanos , Teste de Materiais
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