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J Ethnopharmacol ; 237: 47-54, 2019 Jun 12.
Artigo em Inglês | MEDLINE | ID: mdl-30898554


ETHNOPHARMACOLOGICAL RELEVANCE: The quality control of Traditional Chinese medicine (TCM) is a scientific problem and an industrial issue, which hampers the development of evidence based TCM. The concept of quality markers (Q-markers) is proposed and has been applied to the quality evaluation of TCM based on its clinical efficacy. However, more specific methods are needed to put this idea into practice. The standard decoction is a representative of decoction used in clinical practice and it can be used for the discovery of Q-markers related to the clinical efficacy of TCM. AIM OF THE STUDY: In this study, a systemic strategy was established to discover Q-markers related to the clinical efficacy of TCM Ephedrae Herba (EH), dried stem of Ephedra sinica Stapf. The different processed materials of EH have different clinical applications, though originating from the same medicinal herb. MATERIALS AND METHODS: The standard decoction of each of the processed materials was prepared and a 1HNMR metabolomics approach and total polysaccharide analysis were used to identify potential Q-markers related to the different clinical applications of EH. Correlation analysis was made of the measured biological activity and the holistic chemical profile. RESULTS: The results showed that total polysaccharides and alkaloids were Q-markers for EH preparations. CONCLUSION: This study demonstrates that the standard decoction is a reasonable research objective to explore chemical markers that correlate with the clinical efficacy of TCM.

Ephedra , Extratos Vegetais/farmacologia , Caules de Planta , Controle de Qualidade , Células HEK293 , Humanos , Luciferases/fisiologia , Metabolômica , Regiões Promotoras Genéticas , Receptor 2 Toll-Like/fisiologia , Fator de Transcrição AP-1/fisiologia , Resultado do Tratamento
Zhongguo Zhong Yao Za Zhi ; 42(5): 809-816, 2017 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-28994518


Decoction of single medicinal herb is a reference for the standardization of different dosage form of Chinese medicine and it provides a new direction for solving the problems existing in the quality of Chinese medicinal granules such no uniform dosage forms and no clear quality standard. There are few reports on the idea, method and preparation of single herb standard decoction. Our country is in urgent need of that information in order to improve the consistency and stability of traditional Chinese medicine products. Here, Lonicerae Japinicae Flos was selected as an example to elucidate the preparation and quality evaluation of Chinese single herbal medicine decoction. Twelve batches of representative Lonicerae Japinicae Flos were collected, UPLC fingerprints were established, and the chemical structures of main peaks were identified with UPLC-QTOF-MS and standard compounds. The main components in the decoction are organic acids and iridoids. The extract rate of the standard decoction was (34.2±2.9)% and the transfer rate is (78.6±8.4)% in the form of chlorogenic acid, within the range of 75%-125% of mean. This paper established a method for the quality evaluation of standard decoction of Lonicerae Japinicae Flos and provided reference for the quality control method of terminal products from decoction of Lonicerae Japinicae Flos.

Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/normas , Lonicera/química , Ácido Clorogênico , Cromatografia Líquida de Alta Pressão , Flores/química , Extratos Vegetais/química , Extratos Vegetais/normas , Plantas Medicinais/química , Controle de Qualidade
Zhongguo Zhong Yao Za Zhi ; 42(5): 823-829, 2017 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-28994520


To establish the quality control methods for the standard decoction of Ephedrae Herba, and provide the reference for quality evaluation method of all Chinese herbal medicine decoction.Standard decoction of Ephedrae Herba was prepared, and UPLC-UV fingerprint was established to determine the total contents of ephedrine and pseudoephedrine. Then UPLC-QTOF/MS was used to confirm the major common peaks in the fingerprint to clarify the main chemical constituents in the decoction. In addition, the stability of the process was evaluated by calculating the parameters such as the extraction ratio, transfer rate of the index components and the pH values.In the decoction of Ephedrae Herba, the total average concentration of ephedrine and pseudoephedrine was (2.11±0.70) g•L⁻¹; the similarities of all the fingerprints were more than 0.85; there were 10 major common peaks in the fingerprint, including alkaloids, flavonoids and organic acids; the extraction ratio was (17±3.2)%, and the overall transfer rate of ephedrine and pseudoephedrine was (32.4±8.1)%.The method for evaluating the quality of standard decoction of Ephedrae Herba was established in this article, providing reference for the quality control of products which were stemmed from the water extract of Ephedrae Herba.

Medicamentos de Ervas Chinesas/química , Medicamentos de Ervas Chinesas/normas , Ephedra/química , Alcaloides , Cromatografia Líquida de Alta Pressão , Efedrina/análise , Espectrometria de Massas , Extratos Vegetais/química , Extratos Vegetais/normas , Pseudoefedrina/análise , Controle de Qualidade
J Pharm Biomed Anal ; 132: 77-86, 2017 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-27697573


Chronic atrophic gastritis (CAG) is one of the most important pre-cancerous states with a high prevalence. Exploring of the underlying mechanism and potential biomarkers is of significant importance for CAG. In the present work, 1H NMR-based metabonomics with correlative analysis was performed to analyze the metabolic features of CAG. 19 plasma metabolites and 18 urine metabolites were enrolled to construct the circulatory and excretory metabolome of CAG, which was in response to alterations of energy metabolism, inflammation, immune dysfunction, as well as oxidative stress. 7 plasma biomarkers and 7 urine biomarkers were screened to elucidate the pathogenesis of CAG based on the further correlation analysis with biochemical indexes. Finally, 3 plasma biomarkers (arginine, succinate and 3-hydroxybutyrate) and 2 urine biomarkers (α-ketoglutarate and valine) highlighted the potential to indicate risks of CAG in virtue of correlation with pepsin activity and ROC analysis. Here, our results paved a way for elucidating the underlying mechanisms in the development of CAG, and provided new avenues for the diagnosis of CAG and presented potential drug targets for treatment of CAG.

Biomarcadores/metabolismo , Gastrite Atrófica/metabolismo , Metabolômica/métodos , Ácido 3-Hidroxibutírico/metabolismo , Animais , Arginina/química , Biomarcadores/urina , Peso Corporal , Doença Crônica , Progressão da Doença , Ácidos Graxos/metabolismo , Gastrite Atrófica/urina , Glucose/metabolismo , Sistema Imunitário , Inflamação , Mucosa Intestinal/metabolismo , Ácidos Cetoglutáricos/metabolismo , Lipídeos/química , Espectroscopia de Ressonância Magnética , Masculino , Análise Multivariada , Estresse Oxidativo , Pepsina A/química , Prolina/química , Curva ROC , Ratos , Ratos Sprague-Dawley