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1.
Mitochondrial DNA B Resour ; 6(11): 3156-3158, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34746391

RESUMO

Styrax serrulatus Roxburgh (William Roxburgh 1832), which plays an important role in ecology and economy, is a deciduous species of Styracaceae. In this paper, we sequenced, assembled, and annotated the chloroplast (cp) genome of S. serrulatus by using the sequencing data from Illumina Novaseq platform (Illumina, San Diego, CA). The complete cp genome of S. serrulatus is 157,929 base pairs (bp) in length, containing a pair of inverted repeat regions (IRs) of 26,048 bp each, a large single-copy (LSC) region of 87,552 bp, and a small single-copy (SSC) region of 18,281 bp. It contains 133 genes, including 8 rRNA genes, 37 tRNA genes, 87 protein-coding genes, and 1 pseudo gene. The GC content of S. serrulatus cp genome is 36.96%. The phylogenetic analysis suggests that S. serrulatus is a sister species to Styrax agrestis in Styracaceae.

2.
BMC Med Inform Decis Mak ; 21(1): 284, 2021 10 16.
Artigo em Inglês | MEDLINE | ID: mdl-34656109

RESUMO

Charging according to disease is an important way to effectively promote the reform of medical insurance mechanism, reasonably allocate medical resources and reduce the burden of patients, and it is also an important direction of medical development at home and abroad. The cost forecast of single disease can not only find the potential influence and driving factors, but also estimate the active cost, and tell the management and reasonable allocation of medical resources. In this paper, a method of Bayesian network combined with regression analysis is proposed to predict the cost of treatment based on the patient's electronic medical record when the amount of data is small. Firstly, a set of text-based medical record data conversion method is established, and in the clustering method, the missing value interpolation is carried out by weighted method according to the distance, which completes the data preparation and processing for the realization of data prediction. Then, aiming at the problem of low prediction accuracy of traditional regression model, this paper establishes a prediction model combined with local weight regression method after Bayesian network interpretation and classification of patients' treatment process. Finally, the model is verified with the medical record data provided by the hospital, and the results show that the model has higher prediction accuracy.


Assuntos
Registros Eletrônicos de Saúde , Custos de Cuidados de Saúde , Teorema de Bayes , Análise por Conglomerados , Humanos , Análise de Regressão
3.
Mitochondrial DNA B Resour ; 6(10): 2975-2976, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34553064

RESUMO

Xylosma racemosa (Siebold & Zucc.) Miq. which plays an important role in ecology, medicine and economy, is a deciduous species of Salicaceae. In this paper, we sequenced, assembled, and annotated the chloroplast (cp) genome of X. racemosa (Siebold & Zucc.) Miq. by using the sequencing data from Illumina Novaseq platform. The complete cp genome of X. racemosa is 157,262 bp in length, containing a large single-copy (LSC) region of 84,289 bp, and a small single-copy (SSC) region of 17,817 bp. It contains 131 genes, including 8 rRNA genes, 37 tRNAs genes and 86 protein-coding genes. The GC content of X. racemosa cp genome is 36.74%. The phylogenetic analysis suggests that X. racemosa is a sister species to Xylosma congesta in Salicaceae.

4.
ACS Appl Mater Interfaces ; 13(36): 43438-43448, 2021 Sep 15.
Artigo em Inglês | MEDLINE | ID: mdl-34465082

RESUMO

Accurate detection, quantitation, and differentiation of polycyclic aromatic hydrocarbons (PAHs) and their isomers in diverse samples is elusive for paper spray ionization mass spectrometry (PSI-MS). To address these issues, herein, for the first time, we propose to fabricate a novel, flexible, and stable paper substrate based on covalent organic frameworks (COFs) via an in situ method under room temperature in air. After embedding gold nanoparticles (AuNPs), this paper substrate (COFs-paper) could further serve as a multifunctional plasmonic matrix (AuNPs-COFs-paper) for dual-wavelength laser-assisted PSI-MS detection of PAHs and feasible paper surface-enhanced Raman scattering (pSERS)-aided isomer discrimination. Taking advantage of the synergistic effect between the AuNPs and COFs present on the novel AuNP-embedded COFs-paper substrate, a satisfied LOD of 0.50 ng/µL for phenanthrene was realized, which improved almost 300 times compared with the naked-paper matrix, and the regression coefficient R2 was up to 0.999. Real sample corn oil-containing PAHs can be efficiently detected and identified using this technique. The established platform has promising potential for on-site chemical analysis with portable PSI-MS and pSERS instruments.

5.
Ecotoxicol Environ Saf ; 224: 112676, 2021 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-34419644

RESUMO

Chemical hazardous substances in teethers may migrate into infant's body through oral exposure, resulting in a potential health risk. In recent years, researchers have performed a series of studies for detecting target chemicals in teethers and other toys, but the presence of unknown chemicals has not been systematically investigated yet. This paper reports the non-targeted identification of unknown chemical hazards that may have migrated from teethers to infants based on gas chromatography-Orbitrap high resolution mass spectrometry. In view of the difficulties that may be encountered in the qualitative analysis of substances, several typical cases and the corresponding reliable solutions are given from the perspective of comprehensive score and retention index, isotope-aided qualitative analysis, chemical ionization identification formula, and fragment ion detail comparison for distinguishing isomers. Finally, 28 substances are identified in 10 teether samples. Among them, phenol, N-methylaniline, 1,6-dioxacyclododecane-7,12-dione and cyclohexanone have higher detection rates. This study not only has valuable reference for the identification of unknown substances, but also has positive guiding role in monitoring potential chemical hazards in toys and promoting the safety of products.

6.
Chem Commun (Camb) ; 57(54): 6612-6615, 2021 Jul 06.
Artigo em Inglês | MEDLINE | ID: mdl-34116566

RESUMO

Negative ion mode paper spray mass spectrometry (PS-MS) suffers from intense background noise and unstable MS signal. For the first time, we reported fluorinated boron nitride nanosheet (h-FBN) assisted negative ion PS-MS for the detection of a series of molecules. We demonstrated that the introduction of h-FBN can greatly improve the detection sensitivity and signal stability in the negative ion mode.


Assuntos
Compostos de Boro/química , Halogenação , Limite de Detecção , Espectrometria de Massas/métodos , Papel , Nanoestruturas/química , Razão Sinal-Ruído
7.
Proc Natl Acad Sci U S A ; 118(23)2021 06 08.
Artigo em Inglês | MEDLINE | ID: mdl-34074749

RESUMO

Ataxia telangiectasia and Rad3 related (ATR) activation after replication stress involves a cascade of reactions, including replication protein A (RPA) complex loading onto single-stranded DNA and ATR activator loading onto chromatin. The contribution of histone modifications to ATR activation, however, is unclear. Here, we report that H3K14 trimethylation responds to replication stress by enhancing ATR activation. First, we confirmed that H3K14 monomethylation, dimethylation, and trimethylation all exist in mammalian cells, and that both SUV39H1 and SETD2 methyltransferases can catalyze H3K14 trimethylation in vivo and in vitro. Interestingly, SETD2-mediated H3K14 trimethylation markedly increases in response to replication stress induced with hydroxyurea, a replication stress inducer. Under these conditions, SETD2-mediated H3K14me3 recruited the RPA complex to chromatin via a direct interaction with RPA70. The increase in H3K14me3 levels was abolished, and RPA loading was attenuated when SETD2 was depleted or H3K14 was mutated. Rather, the cells were sensitive to replication stress such that the replication forks failed to restart, and cell-cycle progression was delayed. These findings help us understand how H3K14 trimethylation links replication stress with ATR activation.


Assuntos
Proteínas Mutadas de Ataxia Telangiectasia/metabolismo , Replicação do DNA , DNA/biossíntese , Histona-Lisina N-Metiltransferase/metabolismo , Histonas/metabolismo , Proteína de Replicação A/metabolismo , Animais , Proteínas Mutadas de Ataxia Telangiectasia/química , Proteínas Mutadas de Ataxia Telangiectasia/genética , DNA/química , DNA/genética , Histona-Lisina N-Metiltransferase/química , Histona-Lisina N-Metiltransferase/genética , Histonas/química , Histonas/genética , Humanos , Metilação , Metiltransferases/química , Metiltransferases/genética , Metiltransferases/metabolismo , Proteína de Replicação A/química , Proteína de Replicação A/genética , Proteínas Repressoras/química , Proteínas Repressoras/genética , Proteínas Repressoras/metabolismo
8.
Mitochondrial DNA B Resour ; 6(3): 774-775, 2021 Mar 11.
Artigo em Inglês | MEDLINE | ID: mdl-33763575

RESUMO

Styrax agrestis (Lour.) G. Don, is a deciduous species of Styracaceae with beautiful shape, drooping flowers, and blooming like snow. Here, we characterized the complete chloroplast (cp) genome of S. agrestis using next generation sequencing. The circular complete cp genome of S. agrestis is 157,893 bp in length, containing a large single-copy (LSC) region of 87,512 bp, and a small single-copy (SSC) region of 18,285 bp. It comprises 136 genes, including eight rRNA genes, 37 tRNAs genes, 90 protein-coding genes, and one pseudo gene. The GC content of S. agrestis cp genome is 36.96%. The phylogenetic analysis suggests that S. agrestis is a sister species to Styrax faberi in Styracaceae.

9.
Mitochondrial DNA B Resour ; 6(1): 205-207, 2021 Jan 21.
Artigo em Inglês | MEDLINE | ID: mdl-33537445

RESUMO

Huodendron tibeticum (J.Anthony) Rehder, which plays an important role in ecology and economy, is a deciduous species of Styracaceae. The authors sequenced, assembled, and annotated the chloroplast (cp) genome of Huodendron tibeticum using the sequencing data from Illumina Novaseq platform in this study. The complete cp genome of H. tibeticum is 159,320 bp in length, including a large single-copy (LSC) region of 87,795 bp, and a small single-copy (SSC) region of 18,989 bp. It contains 130 genes, including 37 tRNA genes, 8 rRNA genes, and 85 protein-coding genes. The overall GC content of H. tibeticum chloroplast genome is 36.66%. The phylogenetic analysis suggests that H. tibeticum is a sister species to H. biaristatum in Styracaceae.

11.
Anal Chem ; 93(3): 1749-1756, 2021 01 26.
Artigo em Inglês | MEDLINE | ID: mdl-33351590

RESUMO

Significant efforts have been made to develop robust and reliable methods for simultaneous biothiols determination in different matrices, but there still exist the problems such as easy oxidation, tedious derivatization, and difficulty in discrimination, which brings unsatisfactory results in their accuracy and fast quantification in biological samples. To overcome these problems, a simultaneous biothiols detection method combining a "selenium signature" chemical probe and paper spray mass spectrometry (PS-MS) was proposed. In the strategy, the modified-paper substrate is used to enhance the analytical performance. Chemical probe Ebselen-NH2 that has a specific response to biothiols was designed and covalently fixed on the surface of an oxidized paper substrate. By the identification of derivatized product with distinctive selenium isotope distribution and employment of the optimized PS-MS method, qualitative and quantitative analysis of five biothiols including glutathione (GSH), cysteine (Cys), cysteinylglycine (CysGly), N-acetylcysteine (Nac), and homocysteine (Hcy) were realized. Biothiols in plasma and cell lysates were measured with satisfactory results. The established method not only provides a novel protocol for simultaneous determination of biothiols, but also is helpful for understanding the biological and clinical roles played by these bioactive small molecules.


Assuntos
Acetilcisteína/análise , Cisteína/análise , Dipeptídeos/análise , Corantes Fluorescentes/química , Glutationa/análise , Papel , Selênio/química , Técnicas Biossensoriais , Cisteína/análogos & derivados , Humanos , Espectrometria de Massas
12.
Talanta ; 223(Pt 2): 121745, 2021 Feb 01.
Artigo em Inglês | MEDLINE | ID: mdl-33298269

RESUMO

The near-infrared fluorescence of gold nanoclusters stabilized with bovine serum albumin (BSA -AuNCs) centered at 675 nm could be enhanced by cysteine and then effectively quenched by copper ion (Cu2+), therefore, cysteine and copper ion could be detected in sequence. At "on" state, fluorescence enhancement of BSA-AuNCs is generated due to the reaction between cysteine and BSA-AuNCs, via filling the surface defect of gold nanoclusters, while Cu2+ can further oxidize the reductive sulfydryl of cysteine and interact with amino acids presented in the BSA chain, inducing gold nanoclusters to aggregate, thus causing "off" state with fluorescence quenching. Fluorescence switch of BSA-AuNCs can be used for cysteine and Cu2+ detection in mice brain with Alzheimer's disease (AD) in vitro, with fast response, high chemical stability and sensitivity. Besides, it was able to image the endogenous Cu2+ in liver and heart of AD mice in situ. The results are promising, especially in the framework of early diagnosis of Alzheimer's disease.


Assuntos
Doença de Alzheimer , Nanopartículas Metálicas , Doença de Alzheimer/diagnóstico por imagem , Animais , Cobre , Cisteína , Fluorescência , Ouro , Camundongos , Soroalbumina Bovina , Espectrometria de Fluorescência
13.
Mitochondrial DNA B Resour ; 5(1): 564-565, 2020 Jan 14.
Artigo em Inglês | MEDLINE | ID: mdl-33366648

RESUMO

Styrax obassia Siebold & Zucc. is a distinct member of the family Styracaceae with fragrant and beautiful flowers. In this study, we determined the complete chloroplast (cp) genome sequence of S. obassia in an effort to provide genomic resources useful for promoting its conservation. The circular genome of S. obassia was 157,910 bp in size and contained two inverted repeat (IRa and IRb) regions of 26,051 bp, which were separated by a large single copy (LSC) region of 87,528 bp, and a small single copy (SSC) region of 18,280 bp. A total of 133 genes are encoded, including 88 protein-coding genes, 37 tRNA genes, and eight rRNA genes. The overall GC content of S. obassia genome is 36.97%. The phylogenetic analysis suggests that S. obassia is a sister species to Styrax suberifolius and Styrax zhejiangensis in Styracaceae.

14.
Mitochondrial DNA B Resour ; 5(1): 770-771, 2020 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-33366743

RESUMO

Styrax calvescens Perk., is a nice tree, valued for its beauty and fragrance. Here, we characterized the complete chloroplast (cp) genome of S. calvescens using next-generation sequencing. The circular complete cp genome of S. calvescens is 157,951 bp in length, containing a large single-copy (LSC) region of 87,566 bp and a small single-copy (SSC) region of 18,289 bp. It comprises of 133 genes, including 8 rRNA genes, 37 tRNAs genes, and 88 protein-coding genes. The GC content of S. calvescens cp genome is 36.95%. The phylogenetic analysis suggests that S. calvescens is a sister species to Styrax grandiflorus Griffith in Styracaceae.

15.
Mitochondrial DNA B Resour ; 5(1): 804-805, 2020 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-33366759

RESUMO

Styrax hemsleyanus is valued for its beauty and fragrance. Here, we characterized the complete chloroplast (cp) genome of S. hemsleyanus using next generation sequencing. The circular complete cp genome of S. hemsleyanus is 158,027 bp in length, containing a large single-copy (LSC) region of 87,641 bp, and a small single-copy (SSC) region of 18,290 bp. It comprises 133 genes, including 8 rRNA genes, 37 tRNAs genes, and 88 protein-coding genes. The GC content of S. hemsleyanus cp genome is 36.95%. The phylogenetic analysis suggests that S. hemsleyanus is a sister species to Styrax odoratissimus in Styracaceae.

16.
Mitochondrial DNA B Resour ; 5(1): 828-829, 2020 Jan 24.
Artigo em Inglês | MEDLINE | ID: mdl-33366770

RESUMO

Styrax confusus Hemsl. is valued for its beauty and fragrance. Here, we characterized the complete chloroplast (cp) genome of S. confusus using next-generation sequencing. The circular complete cp genome of S. confusus was 157,981 bp in length, containing a large single-copy (LSC) region of 87,571 bp, and a small single-copy (SSC) region of 18,316 bp. It comprises 133 genes, including eight rRNA genes, 37 tRNA genes, and 88 protein-coding genes. The GC content of S. confusus cp genome is 36.95%. A phylogenetic tree reconstructed by 30 chloroplast genomes reveals that S. confusus is mostly related to Styrax calvescens Perk.

17.
Mitochondrial DNA B Resour ; 5(1): 961-962, 2020 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-33366827

RESUMO

In this work, next-generation sequencing was applied to measure the complete chloroplast genome of S. dasyanthus for the sake of offering valuable genomic information to promote its conservation. The complete chloroplast genome sequence of S. dasyanthus was measured as 157,501 bp in size. It contains the typical structure and gene content of angiosperm plastome, which includes a large single-copy (LSC), a small single-copy (SSC), and 2 inverted repeat (IR) regions of 87,130 bp, 18,277 bp, and 26,047 bp, respectively. The S. dasyanthus chloroplast genome has a total of 130 genes including 85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. Overall, GC contents of the genome were 36.96%. Phylogenetic analysis based on the 29 chloroplast genome measurement demonstrated that S. dasyanthus is most closely related to Styrax grandiflours Griff.

18.
Mitochondrial DNA B Resour ; 5(1): 1029-1030, 2020 Feb 03.
Artigo em Inglês | MEDLINE | ID: mdl-33366860

RESUMO

The object of this work was to measure the complete chloroplast genome of Styrax duclouxii Perkins for the sake of offering valuable genomic information to promote its conservation. The circular genome of S. duclouxii was measured as 157,913 bp in size and contained two inverted repeat (IRa and IRb) regions of 26,040 bp, which were separated by a large single-copy (LSC) region of 87,604 bp and a small single copy (SSC) region of 18,229 bp. A total of 134 genes are encoded, including 89 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The overall GC content of Schizosaccharomyces japonicus genome is 36.97%. A phylogenetic tree reconstructed using 36 chloroplast genomes reveals that S. duclouxii is most closely related with Styrax zhejiangensis and Styrax faberi.

19.
BMC Pregnancy Childbirth ; 20(1): 614, 2020 Oct 12.
Artigo em Inglês | MEDLINE | ID: mdl-33046002

RESUMO

BACKGROUND: Fetal double aortic arch (DAA) malformation is a rare congenital heart disease with few reported cases in the literature. We aimed to investigate the characteristics of prenatal ultrasound and postnatal computed tomography angiography (CTA) of DAA and to describe the associated anomalies and clinical outcomes to improve prenatal diagnosis and assist in perinatal management. METHODS: The obstetric ultrasound imaging databases of seven tertiary referral centers were reviewed retrospectively to identify fetuses with a prenatal diagnosis of DAA between January 2013 and December 2018. Ultrasonographic findings, associated anomalies, genetic abnormalities, postnatal CTA images, and long-term postnatal outcomes were evaluated. RESULTS: A total of 36 cases out of 40 prenatally diagnosed DAA fetuses were confirmed by postnatal diagnosis (fetal autopsy, CTA, and surgery). In this cohort of 36 confirmed cases, 24 (67%) were isolated anomalies, while 12 (33%) were associated with intracardiac or extracardiac anomalies, and 2 (6%) had a 22q11.2 chromosome deletion. Among nine cases of pregnancy termination with a fetal autopsy, 7 had other abnormalities. Among the remaining 27 live births, 16 (59%) were asymptomatic and 11 (41%) received surgical treatment due to tracheal or esophageal compression symptoms, all with satisfactory outcomes. Prenatal echocardiography showed that DAA was mainly characterized by a bifurcation of the ascending aorta into the right and left aortic arch and the formation of a complete O-shaped vascular ring around the trachea on the three-vessel tracheal view. A variant in the aortic arch branching pattern was found for the first time. The airway obstruction, branching pattern, and atretic arch of DAA were clearly shown by postnatal CTA. CONCLUSIONS: Fetal DAA has unique features on prenatal echocardiography and postnatal CTA, and systematic prenatal examination and timely postnatal CTA evaluation are required. A certain proportion of intracardiac and extracardiac abnormalities are associated with DAA, but the probability of chromosome abnormalities is low, especially for isolated DAA.The clinical outcomes of isolated DAA are favorable, even if surgery is performed due to symptoms. Determining whether other malformations or chromosomal anomalies exist is crucial for prognosis evaluation and prenatal counseling.


Assuntos
Obstrução das Vias Respiratórias/diagnóstico , Angiografia por Tomografia Computadorizada , Ecocardiografia Doppler em Cores , Ultrassonografia Pré-Natal/métodos , Anel Vascular/diagnóstico , Aborto Induzido/estatística & dados numéricos , Aborto Terapêutico , Adulto , Obstrução das Vias Respiratórias/etiologia , Obstrução das Vias Respiratórias/cirurgia , Aorta/anormalidades , Aorta/diagnóstico por imagem , Erros de Diagnóstico/estatística & dados numéricos , Feminino , Seguimentos , Humanos , Imageamento Tridimensional , Incidência , Recém-Nascido , Idade Materna , Cuidado Pós-Natal , Estudos Retrospectivos , Resultado do Tratamento , Anel Vascular/complicações , Anel Vascular/epidemiologia , Anel Vascular/cirurgia , Adulto Jovem
20.
Ann Palliat Med ; 9(5): 2575-2585, 2020 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-32921070

RESUMO

BACKGROUND: Autoimmune encephalitis is complex and varied, but it is a curable disease. However, the diagnosis and treatment of children with Autoimmune encephalitis remains challenging. Therefore, we conducted this study to analyze the clinical features, electroencephalogram (EEG) characteristics, treatment and prognosis of autoimmune encephalitis in children with negative and positive anti-N-methyl-D-aspartate receptor (NMDAR) antibody. METHODS: From January 2015 to January 2017, 28 child patients with autoimmune encephalitis were hospitalized in the Neural Ward of the Children's Medical Center, Qilu Hospital of Shandong University. Inclusion criteria were based on the diagnostic criteria for autoimmune encephalitis published in Lancet Neurology in 2016. The clinical, EEG and imaging data were summarized. The clinical features, treatment regimen, follow-up and prognosis were also analyzed. RESULTS: Among these 28 child patients, 10 patients had positive anti-NMDAR antibody, while 18 patients had negative anti-NMDAR antibody. The clinical manifestations, EEG findings and seizures were similar (P>0.05) between these two groups. All 28 child patients were treated with methylprednisolone shock and human immunoglobulin. The response to immunotherapy was similar between these two groups (P>0.05). CONCLUSIONS: The clinical manifestation of autoimmune encephalitis is complex and varied, but it is a curable disease. Immunotherapy should be considered as soon as possible, with or without autoantibodies. Most of the child patients had a good prognosis, while some of them had the sequelae of epilepsy, mild mental symptoms, and dyskinesia. It is necessary to improve the understanding of autoimmune encephalitis with/without positive antibodies, and make diagnosis and treatment as soon as possible, in order to improve the prognosis.


Assuntos
Encefalite Antirreceptor de N-Metil-D-Aspartato , Doença de Hashimoto , Encefalite Antirreceptor de N-Metil-D-Aspartato/diagnóstico , Criança , Eletroencefalografia , Encefalite , Doença de Hashimoto/diagnóstico , Humanos , Receptores de Aminoácido
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