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1.
Mol Med Rep ; 20(4): 3951-3958, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31485663

RESUMO

Polygonatum sibiricum polysaccharide (PSP) has been shown to alleviate hyperglycemia and reduce oxidative stress to delay the progression of diabetic retinopathy and cataracts. However, its role and underlying mechanisms in regulating type 2 diabetes mellitus (T2DM) remain unclear. Nuclear factor erythroid 2­related factor 2 (Nrf2) activation plays a protective role in T2DM. The present study focused on the effect of PSP on inflammatory cytokine secretion and Nrf2 expression in the adipocytes of T2DM patients. In this study, high­glucose­ and high­insulin­induced 3T3­L1 adipocytes were used to mimic insulin­resistant (IR)­3T3­L1 adipocytes. Furthermore, the effect and underlying mechanisms of PSP on inflammation and glucose uptake in IR­3T3­L1 adipocytes were investigated. The present study found that proliferation after 50, 100 and 250 µg/ml PSP treatment had no significant change in normal 3T3­L1 adipocytes. A total of 50, 100 and 250 µg/ml of PSP also alleviated IL­1ß, IL­6, and TNF­α levels and promoted proliferation, glucose uptake, and glucose transporter 4 expression in IR­3T3­L1 adipocytes. Furthermore, 50, 100 and 250 µg/ml PSP promoted Nrf2 and HO­1 expression. However, silencing Nrf2 expression reversed the effect of 100 µg/ml PSP in IR­3T3­L1 adipocytes. In conclusion, these results suggest that PSP alleviates inflammatory cytokines and promotes glucose uptake in IR­3T3­L1 adipocytes by promoting Nrf2 expression. PSP may be a potential therapeutic agent for T2DM treatment by promoting Nrf2 expression.

2.
J Transl Med ; 17(1): 90, 2019 Mar 18.
Artigo em Inglês | MEDLINE | ID: mdl-30885207

RESUMO

BACKGROUND: Hypercoagulation and neutrophilia are described in several cancers, however, whether they are involved in lung carcinogenesis is currently unknown. Emodin is the main bioactive component from Rheum palmatum and has many medicinal values, such as anti-inflammation and anticancer. This study is to investigate the contributions of neutrophils to the effects of emodin on hypercoagulation and carcinogenesis. METHODS: The effects of emodin on neutrophil phenotypes were assessed by cell proliferation, morphological changes, phagocytosis and autophagy in vitro. The anti-coagulation and cancer-preventing actions of emodin were evaluated in the urethane-induced lung carcinogenic model. The expressions of Cit-H3 and PAD4 in lung sections were assessed by immunohistochemistry, CD66b+ neutrophils were distinguished by immunofluorescence, and cytokines and ROS were examined with ELISA. The neutrophils-regulating and hypercoagulation-improving efficacies of emodin were confirmed in a Lewis lung cancer allograft model. The related targets and pathways of emodin were predicted by network pharmacology. RESULTS: In vitro, emodin at the dose of 20 µM had no effect on cell viability in HL-60N1 but increased ROS and decreased autophagy and thus induced apoptosis in HL-60N2 with the morphological changes. In the urethane-induced lung carcinogenic model, before lung carcinogenesis, urethane induced obvious hypercoagulation which was positively correlated with lung N2 neutrophils. There were the aggravated hypercoagulation and lung N2 neutrophils after lung carcinoma lesions. Emodin treatment resulted in the ameliorated hypercoagulation and lung carcinogenesis accompanied by the decreased N2 neutrophils (CD66b+) in the alveolar cavity. ELISA showed that there were more IFN-γ, IL-12 and ROS and less IL-6, TNF-α and TGF-ß1 in the alveolar cavity in the emodin group than those in the control group. Immunohistochemical analysis showed that emodin treatment decreased Cit-H3 and PAD4 in lung sections. In the Lewis lung cancer allograft model, emodin inhibits tumor growth accompanied by the attenuated coagulation and intratumor N2 neutrophils. Network pharmacology indicated the multi-target roles of emodin in N2 neutrophil activation. CONCLUSIONS: This study suggests a novel function of emodin, whereby it selectively suppresses N2 neutrophils to prevent hypercoagulation and lung carcinogenesis.

3.
PLoS One ; 9(11): e112683, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25393306

RESUMO

Epigallocatechin gallate (EGCG) is a major polyphenol in green tea with beneficial effects on the impairment in learning and memory. Autophagy is a cellular process that protects neurons from stressful conditions. The present study was designed to investigate whether EGCG can rescue chronic unpredictable mild stress (CUMS)-induced cognitive impairment in rats and whether its protective effect involves improvement of autophagic flux. As expected, our results showed that CUMS significantly impaired memory performance and inhibited autophagic flux as indicated by elevated LC3-II and p62 protein levels. At the same time, we observed an increased neuronal loss and activated mammalian target of rapamycin (mTOR)/p70 ribosomal protein S6 kinase (p70S6k) signaling in the CA1 regions. Interestingly, chronic treatment with EGCG (25 mg/kg, i.p.) significantly improved those behavioral alterations, attenuated histopathological abnormalities in hippocampal CA1 regions, reduced amyloid beta1-42 (Aß1-42) levels, and restored autophagic flux. However, blocking autophagic flux with chloroquine, an inhibitor of autophagic flux, reversed these effects of EGCG. Taken together, these findings suggest that the impaired autophagy in CA1 regions of CUMS rats may contribute to learning and memory impairment. Therefore, we conclude that EGCG attenuation of CUMS-induced learning and memory impairment may be through rescuing autophagic flux.


Assuntos
Catequina/análogos & derivados , Disfunção Cognitiva/tratamento farmacológico , Aprendizagem em Labirinto/efeitos dos fármacos , Memória/efeitos dos fármacos , Nootrópicos/farmacologia , Estresse Psicológico/tratamento farmacológico , Peptídeos beta-Amiloides/genética , Peptídeos beta-Amiloides/metabolismo , Animais , Autoantígenos/genética , Autoantígenos/metabolismo , Autofagia/efeitos dos fármacos , Região CA1 Hipocampal/efeitos dos fármacos , Região CA1 Hipocampal/metabolismo , Região CA1 Hipocampal/patologia , Catequina/antagonistas & inibidores , Catequina/farmacologia , Cloroquina/farmacologia , Doença Crônica , Disfunção Cognitiva/etiologia , Disfunção Cognitiva/genética , Disfunção Cognitiva/fisiopatologia , Regulação da Expressão Gênica , Masculino , Proteínas Associadas aos Microtúbulos/genética , Proteínas Associadas aos Microtúbulos/metabolismo , Neurônios/efeitos dos fármacos , Neurônios/metabolismo , Neurônios/patologia , Nootrópicos/antagonistas & inibidores , Fragmentos de Peptídeos/genética , Fragmentos de Peptídeos/metabolismo , Ratos , Ratos Wistar , Proteínas Quinases S6 Ribossômicas 70-kDa/genética , Proteínas Quinases S6 Ribossômicas 70-kDa/metabolismo , Transdução de Sinais , Estresse Psicológico/complicações , Estresse Psicológico/genética , Estresse Psicológico/fisiopatologia , Serina-Treonina Quinases TOR/genética , Serina-Treonina Quinases TOR/metabolismo
4.
Acta Crystallogr Sect E Struct Rep Online ; 68(Pt 6): o1639, 2012 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-22719440

RESUMO

In the title compound, C(20)H(13)ClN(2)O(2)S, the chloro-phenyl, phenyl and thienoyl rings are oriented at dihedral angles 17.84 (7), 53.13 (8) and 34.03 (8)°, respectively, to the central pyrazole ring. An intra-molecular O-H⋯O hydrogen bond occurs. In the crystal, pairs of bifurcated O-H⋯O hydrogen bonds link mol-ecules into inversion dimers with R(2) (2)(12) graph-set motifs.

5.
Zhong Yao Cai ; 31(2): 229-32, 2008 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-18619268

RESUMO

OBJECTIVE: To study on volatice oil from Atractylodes macrosephala Koidz with different distill methods and find the better method. METHODS: GC-MS was used to analyze the chemical constituents of volatice oil from Atractylodes macrocephala Koidz with different distill methods. RESULTS: The extraction rates of volatice oil with steam distillation was 1.01%, the components of the oil were examined by GC-MS, 15 of the 18 were identified. The extraction rates of volatice oil with ultrasonic wave was 1.60%, the components examined, 20 of the 24 were identified. The extraction rates of volatice oil with SFE-CO2 was 2.32%, the components examined, 37 of the 49 were identified. Atractylon was the highest one. There were 12 common components in the identified ones. CONCLUSION: The components of volatice oil from Atractylodes macrocephala Koidz with different distill methods have difference but similarities, it can provide a method for Atractylodes macrocephala Koidz's quality control. The extraction rates is higher and the components are more with the method of SFE-CO2.


Assuntos
Atractylodes/química , Óleos Voláteis/isolamento & purificação , Plantas Medicinais/química , Tecnologia Farmacêutica/métodos , Dióxido de Carbono , Cromatografia com Fluido Supercrítico/métodos , Cromatografia Gasosa-Espectrometria de Massas/métodos , Óleos Voláteis/química , Sesquiterpenos/análise , Sesquiterpenos/isolamento & purificação , Vapor , Ultrassom
6.
Zhong Yao Cai ; 28(2): 79-81, 2005 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-15981871

RESUMO

OBJECTIVE: To determine the best manure scheme of lily (Lilium lancifolium). METHODS: Determining lilypolysaccharide and phosphatide contents through phenol hydrate-sulfuric acid and molybdenum blue colorimetric method. RESULTS: The content of efficacious composition in applying fertilizer on the leaves is higher than average and possium fertilizer can increase lilypolysaccharide content. CONCLUSION: Possium fertilizer is important in early stage and leaf fertilizer can improve lily quality.


Assuntos
Fertilizantes , Lilium , Esterco , Plantas Medicinais , Fertilizantes/classificação , Lilium/crescimento & desenvolvimento , Nitrogênio , Fosfolipídeos/análise , Fosfolipídeos/isolamento & purificação , Plantas Medicinais/crescimento & desenvolvimento , Polissacarídeos/análise , Polissacarídeos/isolamento & purificação , Potássio , Controle de Qualidade , Espectrofotometria Ultravioleta , Fatores de Tempo , Ureia
7.
Zhong Yao Cai ; 26(6): 399-400, 2003 Jun.
Artigo em Chinês | MEDLINE | ID: mdl-14528678

RESUMO

OBJECTIVE: To exploit and utilize reasonably the abundant natural resources by appraising the medicinal purposes of Lonicera macranthoides Hands-Mazz. through the content of the useful chemical constitutents chlorogenic acid. METHOD: The content of chlorogenic acid in the flowers of L. macranthoides Hands-Mazz in Human and L. japonica Thunb. in Henan and Shandong was compared by HPLC. RESULT: The contents of chlorogenic acid were 4.00% and 4.52% in L. macrathoides from Longhui and Xinning County of Hunan respectively. The contents were 2.20% and 2.46% in L. japonica from Shangdong and Henan respectively. CONCLUSION: The content of chlorogenic acid in L. macranthoides was higher than that in Lonicera japonica.


Assuntos
Ácido Clorogênico/análise , Lonicera/química , Plantas Medicinais/química , Cromatografia Líquida de Alta Pressão , Flores/química , Lonicera/classificação
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