Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 11 de 11
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Cornea ; 39(1): 56-62, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31356422

RESUMO

PURPOSE: To evaluate custom fast cross-linking (cfCXL) treatment of keratoconus. METHODS: "Custom fast cross-linking" or "cfCXL" is a keratoconus treatment algorithm featuring no epithelial disruption, 15 minutes of corneal presoaking with a riboflavin-vitamin E TPGS solution, and a 370-nm ultraviolet A radiation beam centered on the most highly curved corneal region. Ultraviolet A radiation beam fluence, total energy, and exposure time are significantly less than those in the Dresden protocol. In this study, refraction, spectacle-corrected distance visual acuity, Kmax, and corneal hysteresis were monitored in 81 eyes of 81 patients for 7 years with 100% follow-up. Pretreatment Kmax and patient age averaged 53.01 ± 4.87 D and 25.9 ± 4.7 years, respectively. RESULTS: Average refractive cylinder magnitude was reduced by 26.1% at 1 month postoperatively and by 44.2% at 7 years postoperatively. Logarithm of the minimum angle of resolution average spectacle-corrected distance visual acuity (best spectacle-corrected distance visual acuity) improved from +0.26 ± 0.34 (20/36.4) to +0.15 ± 0.23 (20/28.25), +0.05 ± 0.20 (20/22.4), and +0.06 ± 0.20 (20/22.96) at 1 month, 1 year, and 7 years postoperatively, respectively. Best spectacle-corrected distance visual acuity improved in 54.3%, 74.1%, 84.0%, 87.7%, 84.0%, 84.0%, and 82.7% of patients at postoperative months 1, 3, 6, 12, 24, 48, and 84, respectively. Kmax did not increase in 96.3% of patients at 1 month, 97.5% at 1 year, and 98.8% at 7 years postoperatively, with average corneal apex flattening at 1 month and 7 years of -2.79 ± 1.70 D and -4.00 ± 2.40 D, respectively. CONCLUSIONS: Custom fast cross-linking, epi-on, rapid, narrowed beam apex-centered treatment of keratoconus with riboflavin-vitamin E TPGS produced a significant, rapid, and lasting cone progression stoppage, astigmatism reduction, and visual acuity improvement.

2.
Int Ophthalmol ; 2019 Dec 03.
Artigo em Inglês | MEDLINE | ID: mdl-31792856

RESUMO

PURPOSE: To assess the ocular hypotensive effect of 15-keto fluprostenol, the oxidized metabolite of travoprost, on glaucoma patients, through a randomized double-masked placebo-controlled study. METHODS: Twelve patients with ocular normal tension glaucoma (NTG) (intraocular pressure [IOP] < 22 mmHg) were enrolled. In order to ensure patient compliance to treatment, all study subjects were hospitalized. In each patient, the eye to be submitted to the treatments was randomly chosen. After hospital admission (day 1), those patients received for 5 days at 8 P.M. either one drop of 15-keto fluprostenol (35 µg/ml) or one drop of placebo. IOP evaluation was performed within 8 A.M. and 8 P.M. for 6 days. Furthermore, we performed a determination of cardiovascular parameters before and after the treatments. RESULTS: Starting with the first IOP measurement after the first treatment (8 A.M. on day 2), IOP was reduced of about 14% in the eyes treated 15-keto fluprostenol, in comparison with baseline IOP values of 15-keto fluprostenol-treated patients. The IOP reduction in the 15-keto fluprostenol-treated group was significantly compared to placebo group (p < 0.05) starting from day 3 till day 6 of the study. Except for mild hyperemia in one 15-keto fluprostenol-treated eye, no other side effects were observed or reported by the enrolled patients. CONCLUSIONS: The travoprost metabolite 15-keto fluprostenol was effective in decrease IOP and maintained IOP reduction along 5 days of treatment. The 15-keto fluprostenol can be developed as a good candidate for once-a-day NTG patients' treatment.

3.
Exp Eye Res ; 189: 107848, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31634477

RESUMO

Prophylaxis represents a keystone to reduce periocular skin and ocular conjunctiva bacterial load before surgical procedures. Despite many prophylactic agents are available the preferred perioperative ocular surface antimicrobial is still unknown. The purpose of this study was to assess the effectiveness of preoperative liposomal ozone dispersion in reducing bacterial colonization from the conjunctival sac and periocular skin in dogs, in comparison with povidone-iodine and fluoroquinolone. Twenty-two owned dogs consisting with 44 eyes in total scheduled for ophthalmic surgical procedure were enrolled for the study and divided in four groups receiving either ozone dispersion or povidone iodine in eyelid and conjunctiva, fluoroquinolone or placebo. A swab was taken before and after the antisepsis protocol evaluating total microbial count, coagulase positive and negative staphylococci. Statistical analysis revealed a significant decrease in colony forming units (CFU) for total microbial count, coagulase positive and negative staphylococci both for liposomal ozone dispersion and povidone iodine. No statistical differences were detected in median CFU for both one-day placebo and fluoroquinolone preoperative prophylactic topical therapy. The results of this preliminary study demonstrate that liposomal ozone-dispersion is as effective as povidone iodine to reduce preoperative bacterial load in ocular surface.


Assuntos
Bactérias/isolamento & purificação , Túnica Conjuntiva/microbiologia , Endoftalmite/prevenção & controle , Infecções Oculares Bacterianas/prevenção & controle , Aparelho Lacrimal/microbiologia , Ozônio/administração & dosagem , Infecção da Ferida Cirúrgica/prevenção & controle , Administração Tópica , Animais , Bactérias/efeitos dos fármacos , Túnica Conjuntiva/patologia , Modelos Animais de Doenças , Cães , Endoftalmite/microbiologia , Endoftalmite/patologia , Infecções Oculares Bacterianas/microbiologia , Infecções Oculares Bacterianas/patologia , Feminino , Aparelho Lacrimal/patologia , Lipossomos , Masculino , Procedimentos Cirúrgicos Oftalmológicos , Oxidantes Fotoquímicos/administração & dosagem , Período Pré-Operatório , Estudos Prospectivos , Infecção da Ferida Cirúrgica/microbiologia , Infecção da Ferida Cirúrgica/patologia
4.
Emerg Infect Dis ; 25(2): 372-374, 2019 02.
Artigo em Inglês | MEDLINE | ID: mdl-30666943

RESUMO

We report biomolecular evidence of dolphin morbillivirus in 4 wild Eurasian otters (Lutra lutra) from southern Italy; 2 animals showed simultaneous immunohistochemical reactivity against morbilliviral antigen. These cases add further concern and support to the progressively expanding host range of dolphin morbillivirus in the western Mediterranean Sea.


Assuntos
Doenças dos Animais/epidemiologia , Doenças dos Animais/virologia , Infecções por Morbillivirus/veterinária , Morbillivirus , Lontras/virologia , Doenças dos Animais/patologia , Animais , Golfinhos/virologia , Feminino , Itália/epidemiologia , Morbillivirus/genética
5.
Sci Rep ; 8(1): 11577, 2018 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-30068967

RESUMO

Mass strandings of sperm whales (Physeter macrocephalus) are rare in the Mediterranean Sea. Nevertheless, in 2014 a pod of 7 specimens stranded alive along the Italian coast of the Central Adriatic Sea: 3 individuals died on the beach after a few hours due to internal damages induced by prolonged recumbency; the remaining 4 whales were refloated after great efforts. All the dead animals were genetically related females; one was pregnant. All the animals were infected by dolphin morbillivirus (DMV) and the pregnant whale was also affected by a severe nephropathy due to a large kidney stone. Other analyses ruled out other possible relevant factors related to weather conditions or human activities. The results of multidisciplinary post-mortem analyses revealed that the 7 sperm whales entered the Adriatic Sea encountering adverse weather conditions and then kept heading northward following the pregnant but sick leader of the pod, thereby reaching the stranding site. DMV infection most likely played a crucial role in impairing the health condition and orientation abilities of the whales. They did not steer back towards deeper waters, but eventually stranded along the Central Adriatic Sea coastline, a real trap for sperm whales.


Assuntos
Comportamento Animal , Infecções por Morbillivirus/veterinária , Morbillivirus/isolamento & purificação , Cachalote , Animais , Itália , Mar Mediterrâneo , Infecções por Morbillivirus/patologia
6.
J Med Microbiol ; 63(Pt 2): 325-9, 2014 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-24324028

RESUMO

Since 1994, when Brucella ceti was first isolated from an aborted dolphin fetus, several cases have been reported worldwide. The first case of B. ceti in the Mediterranean (and in Italy), however, was recorded only in 2012, off the coast of Tuscany. Extensive studies, using serological and microbiological methods, have documented this bacterium in dolphins and demonstrated its zoonotic potential. We describe the typing of two B. ceti strains isolated from striped dolphins (Stenella coeruleoalba) stranded on the southern Apulia coastline. B. ceti isolates were conventionally typed, and then genotyped by both the multilocus sequence typing (MLST) and the multilocus variable number of tandem repeats typing (MLVA) methodologies to infer phylogeny and potential epidemiological links between the two cases. The two isolates were identified through MLST analysis as belonging to the common sequence type 26 (ST26), while MLVA analysis, having established that the two isolates have identical profiles, assigned them to a novel genotype within cluster A - a unique representative of a new Mediterranean subcluster. The results thus revealed a link between the two cases studied, demonstrating the usefulness of MLST and MLVA for the epidemiological investigation of brucellae among marine mammals.


Assuntos
Brucella/classificação , Brucella/isolamento & purificação , Brucelose/veterinária , Stenella/microbiologia , Animais , Técnicas de Tipagem Bacteriana , Brucella/genética , Brucella/fisiologia , Brucelose/microbiologia , Análise por Conglomerados , Genótipo , Itália , Masculino , Repetições Minissatélites , Epidemiologia Molecular , Tipagem de Sequências Multilocus
7.
Vector Borne Zoonotic Dis ; 13(10): 769-71, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23808978

RESUMO

During an outbreak of sheep anthrax in Basilicata, southern Italy, the owner of a flock located about 3 km away from the affected farm developed skin lesions attributable to cutaneous anthrax. The DNA extracted from the human scabs confirmed the diagnosis, and a 15-loci multiple locus variable number tandem repeat (VNTR) analysis (MLVA) following single-nucleotide repeat (SNR) analysis yielded the same genotype as that found in the dead sheep. The breeder, who had not had contact with infected or dead animals, reported having been stung by gadflies.


Assuntos
Antraz/transmissão , Bacillus anthracis/isolamento & purificação , Surtos de Doenças , Doenças dos Ovinos/transmissão , Dermatopatias Bacterianas/transmissão , Animais , Antraz/epidemiologia , Antraz/microbiologia , Bacillus anthracis/genética , Genótipo , Humanos , Itália/epidemiologia , Repetições Minissatélites/genética , Ovinos , Doenças dos Ovinos/epidemiologia , Doenças dos Ovinos/microbiologia , Dermatopatias Bacterianas/epidemiologia , Dermatopatias Bacterianas/microbiologia , Zoonoses
8.
Eur J Ophthalmol ; 21(3): 243-50, 2011.
Artigo em Inglês | MEDLINE | ID: mdl-20872359

RESUMO

PURPOSE: To determine the effects of 2 artificial tear formulas on the ocular surface in patients with glaucoma using topical preserved beta-blockers (BB) or prostaglandins (PG). METHODS: This was a prospective, comparative, randomized, double-blind study with a crossover design. Twenty patients with dry eye were divided into 2 groups based on glaucoma treatment: BB (10 subjects) or PG (10 subjects). Each group was administered a 4-week course of unpreserved isotonic (300 mOsm/L) solution containing 0.2% sodium hyaluronate (SH) or a preserved isotonic (295-305 mOsm/L) solution containing 0.5% carboxymethylcellulose and 0.9% glycerin as compatible solute (CMCs) administered QID. After a 2-week washout period, the course of treatment was reversed. The primary efficacy criteria consisted of assessing symptoms according to the Ocular Surface Disease Index© (OSDI); the secondary efficacy criteria consisted of evaluating tear film confocal microscopy, central corneal thickness (CCT), and lissamine corneal and conjunctival staining (Oxford Grading System Score [OGSS]). RESULTS: Within each group, only CMCs induced a significant improvement in OSDI and OGSS compared to baseline values: OSDI -20.5, p<0.0001; OGSS -0.9, p<0.0001. Tear film confocal microscopy improved after treatment, especially in case of patients who were administered CMCs. No difference in CCT was noticed for any subject. CONCLUSIONS: This study demonstrates for the first time that the use of concomitant CMCs in the management of glaucoma undergoing treatment with BB or PG may assist in tear film production and could lead to better compliance with intraocular pressure-lowering medication and ultimately better prognosis.


Assuntos
Antagonistas Adrenérgicos beta/uso terapêutico , Córnea/efeitos dos fármacos , Glaucoma de Ângulo Aberto/tratamento farmacológico , Soluções Oftálmicas/administração & dosagem , Prostaglandinas Sintéticas/uso terapêutico , Lágrimas/química , Carboximetilcelulose Sódica/administração & dosagem , Córnea/patologia , Estudos Cross-Over , Método Duplo-Cego , Quimioterapia Combinada , Síndromes do Olho Seco/complicações , Síndromes do Olho Seco/tratamento farmacológico , Glaucoma de Ângulo Aberto/complicações , Glicerol/administração & dosagem , Humanos , Ácido Hialurônico/administração & dosagem , Pressão Intraocular/efeitos dos fármacos , Soluções Isotônicas/administração & dosagem , Microscopia Confocal , Concentração Osmolar , Conservantes Farmacêuticos/administração & dosagem , Estudos Prospectivos
9.
J Gen Virol ; 89(Pt 12): 3173-6, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19008408

RESUMO

Prion protein gene (PRNP) polymorphisms are involved in modulating the appearance of atypical/Nor98 scrapie in sheep, with the alleles AHQ and AF141RQ strongly associated with occurrence of the disease. The presence of histidine at codon 154 has also been detected in Nor98-affected goats, but statistical analysis of the association between Nor98 and goat PRNP polymorphisms has not been reported previously. Here, a case-control study was carried out on eight Nor98-positive goats and 246 negative herdmates belonging to eight Italian Nor98 scrapie outbreaks. The results revealed that histidine at codon 154 is also strongly associated with the disease in goats.


Assuntos
Códon/genética , Doenças das Cabras/genética , Histidina/genética , Polimorfismo Genético , Príons/genética , Scrapie/genética , Animais , Estudos de Casos e Controles , Predisposição Genética para Doença , Genótipo , Cabras , Mutação , Fatores de Risco
10.
Cornea ; 27(10): 1126-30, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-19034126

RESUMO

PURPOSE: The aim of the study is to evaluate the short-term effects of 2 kinds of artificial tears, both containing 0.4% hyaluronic acid in an aqueous solution yet having different osmolarity, on the typical symptoms of patients suffering from dry eye and on the vitality of corneal and conjunctival epithelial cells. METHODS: A cross-over, randomized, balanced, single-blind study involving 28 patients was divided into 2 treatment groups: group A (unpreserved 0.4% hyaluronic acid eye drops 300 mOsm/L) and group B (unpreserved 0.4% hyaluronic acid eye drops 150 mOsm/L). Both treatments were administered for 7 days, being dosed as a 1 solution drop 4 times daily. After a 1-day wash-out period, the patients in group A passed over to group B and vice versa. RESULTS: Treatment with hypotonic solution gave better results in relieving symptoms, along with a statistically significant improvement (P < 0.001) in the state of the corneoconjunctival epithelium, than the isotonic solution. At the end of the study, 60.7% of the patients declared that they preferred hypotonic solution and only 10.7% preferred isotonic solution; the remaining 28.6% did not notice any difference between the 2 treatments. CONCLUSIONS: By reducing the osmolarity of tear film, the hypotonic solution not only improves the characteristics of tear film and the vitality of the epithelial cells of the cornea and conjunctiva but also proves to be effective in reducing dry eye symptoms.


Assuntos
Síndromes do Olho Seco/tratamento farmacológico , Ácido Hialurônico/administração & dosagem , Soluções Hipotônicas/administração & dosagem , Soluções Oftálmicas/química , Soluções Oftálmicas/uso terapêutico , Sobrevivência Celular , Túnica Conjuntiva/efeitos dos fármacos , Túnica Conjuntiva/patologia , Túnica Conjuntiva/fisiopatologia , Estudos Cross-Over , Síndromes do Olho Seco/fisiopatologia , Células Epiteliais/efeitos dos fármacos , Epitélio Anterior/efeitos dos fármacos , Epitélio Anterior/patologia , Epitélio Anterior/fisiopatologia , Feminino , Humanos , Soluções Isotônicas/administração & dosagem , Masculino , Pessoa de Meia-Idade , Concentração Osmolar , Satisfação do Paciente , Método Simples-Cego , Resultado do Tratamento
11.
J Gen Virol ; 87(Pt 5): 1395-402, 2006 May.
Artigo em Inglês | MEDLINE | ID: mdl-16603543

RESUMO

The association between PrP gene variations and scrapie susceptibility was studied in a single herd of Ionica breed goats. The entire herd comprised 100 animals, 11 of which were clinically affected and showed pathological prion protein (PrP(Sc)) deposition in both their central nervous system (CNS) and lymphoreticular system (LRS). Among asymptomatic goats, nine harboured PrP(Sc) in both CNS and LRS, 19 showed PrP(Sc) only at the LRS level and 61 animals had no PrP(Sc) deposition. Genetic analysis of the PrP gene coding sequence revealed the presence of several polymorphisms, namely G37V, T110P, H143R, R154H, Q222K and P240S. Silent polymorphisms were also found at codons 42, 138, 219 and 232. The effect of PrP polymorphism on scrapie susceptibility was assessed by comparing the genotype distribution at each locus among animals with different pathogenetic and clinical disease stages. Significant differences in the distribution of genotypes were observed for codons 154 and 222, with polymorphism at codon 154 modulating susceptibility to scrapie and lysine at codon 222 being associated with scrapie resistance. The allelic variant encoding lysine at position 222 could be a valuable candidate to select in the framework of appropriate breeding programmes for scrapie resistance in goats.


Assuntos
Proteínas PrPSc/genética , Proteínas PrPSc/isolamento & purificação , Scrapie/etiologia , Alelos , Criação de Animais Domésticos , Animais , Sistema Nervoso Central/química , Cabras , Itália , Tecido Linfoide/química , Lisina , Polimorfismo Genético , Scrapie/genética , Scrapie/prevenção & controle
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA