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Diab Vasc Dis Res ; 15(4): 340-343, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-29392977


BACKGROUND: Inflammation plays a pivotal role in the pathogenesis of diabetes and its complications. Arachidonic acid lipoxygenases have been intensively studied in their role in inflammation in metabolic pathways. Thus, we aimed to explore variants of lipoxygenase genes (arachidonate lipoxygenase genes) in a diabetes adult population using a case-control study design. METHODS: Study population consisted of 1285 elderly participants, 716 of whom had type 2 diabetes mellitus. The control group consisted of non-diabetes individuals with no history of diabetes history and with a glycated haemoglobin <6.5% (<48 mmol/mol)] and fasting plasma glucose levels <126 mg/dL. Blood samples were genotyped on Illumina Infinium PsychArray. Variants of ALOX5, ALOX5AP, ALOX12, ALOX15 were selected. All statistical analyses were undertaken within PLINK and SPSS packages utilising permutation analysis tests. RESULTS: Our findings showed an association of rs9669952 (odds ratio = 0.738, p = 0.013) and rs1132340 (odds ratio = 0.652, p = 0.008) in ALOX5AP and rs11239524 in ALOX5 gene with disease (odds ratio = 0.808, p = 0.038). Rs9315029 which is located near arachidonate ALOX5AP also associated with type 2 diabetes mellitus ( p = 0.025). No variant of ALOX12 and ALOX15 genes associated with disease. CONCLUSION: These results indicate a potential protective role of ALOX5AP and 5-arachidonate lipoxygenase gene in diabetes pathogenesis, indicating further the importance of the relationship between diabetes and inflammation. Larger population studies are required to replicate our findings.

Proteínas Ativadoras de 5-Lipoxigenase/genética , Araquidonato 5-Lipoxigenase/genética , Diabetes Mellitus Tipo 2/genética , Polimorfismo de Nucleotídeo Único , Idoso , Idoso de 80 Anos ou mais , Araquidonato 12-Lipoxigenase/genética , Araquidonato 15-Lipoxigenase/genética , Estudos de Casos e Controles , Diabetes Mellitus Tipo 2/enzimologia , Diabetes Mellitus Tipo 2/epidemiologia , Diabetes Mellitus Tipo 2/prevenção & controle , Feminino , Estudos de Associação Genética , Predisposição Genética para Doença , Grécia/epidemiologia , Humanos , Modelos Logísticos , Masculino , Pessoa de Meia-Idade , Razão de Chances , Fenótipo , Análise de Componente Principal , Fatores de Proteção , Fatores de Risco
Diab Vasc Dis Res ; 15(2): 150-153, 2018 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-29113459


BACKGROUND: The combined effect of vitamin D and parathyroid hormone on glucose homeostasis has not been adequately investigated. The aim of this study was to examine the role of parathyroid hormone/vitamin D axis on glucose homeostasis in elderly persons with prediabetes. METHODS: Patients with prediabetes ( n = 144) and healthy age-matched controls ( n = 81) were included in this cross-sectional study. Study parameters included anthropometric characteristics, morning fasting glucose (fasting plasma glucose), insulin (fasting plasma insulin), parathyroid hormone, 25-hydroxyvitamin D, homeostasis model assessment of insulin resistance and homeostasis model assessment of ß-cell function. Both groups were stratified into subgroups according to vitamin D status and tertiles of parathyroid hormone. RESULTS: Both groups were comparable in terms of body mass index, 25-hydroxyvitamin D and parathyroid hormone status. In the prediabetes group, fasting plasma glucose differed significantly across parathyroid hormone tertiles, increasing from the first to the third tertile ( p = 0.011). There were higher fasting plasma glucose values in participants with vitamin D deficiency/parathyroid hormone third tertile compared to all other groups ( p = 0.031, 0.027 and 0.039, respectively). CONCLUSION: Parathyroid hormone status is associated with impaired glucose homeostasis; hypovitaminosis D combined with high parathyroid hormone concentrations are associated with glycaemic dysregulation in elderly patients with prediabetes.

Glicemia/metabolismo , Hormônio Paratireóideo/uso terapêutico , Estado Pré-Diabético/tratamento farmacológico , Vitamina D/uso terapêutico , Idoso , Idoso de 80 Anos ou mais , Glicemia/efeitos dos fármacos , Índice de Massa Corporal , Estudos Transversais , Jejum , Feminino , Homeostase/efeitos dos fármacos , Homeostase/fisiologia , Humanos , Resistência à Insulina/fisiologia , Masculino , Pessoa de Meia-Idade , Hormônio Paratireóideo/metabolismo , Estado Pré-Diabético/complicações , Estado Pré-Diabético/diagnóstico , Vitamina D/sangue , Deficiência de Vitamina D/sangue
Gene ; 613: 10-13, 2017 May 20.
Artigo em Inglês | MEDLINE | ID: mdl-28257836


Nowadays, obesity is the greatest scourge worldwide, particularly for the developed countries and is a huge burden for the public health. Over the past decade, GWAS have revealed a number of genes associated with obesity. The fat mass and obesity associated (FTO) gene was the first one associated with obesity in a significant number of populations and recent meta-analysis studies confirm this association. FTO is a N-methyladenosine demethylase and in addition to the genetic association, its biological role in the regulation of body weight has been documented. Due to lack of replication regarding FTO association with obesity in the Greek adult population, we analyzed three SNPs, i.e. rs9939609, rs9930506 and rs3751812 in a cohort of 203 adults, comprising of 95 obese, 58 overweight and 50 control individuals. Analysis has shown a significant association for FTO (rs9930506; A/G) 'G' allele with obesity and a difference by 3.2 BMI units between the two homozygotes (AA versus GG). This association, which was detected for the first time in this population, suggests that FTO rs9930506 is a predisposition marker to obesity in the Greek adults, but the results should be taken cautiously due to the limitation of the relatively small sample size of the subjects.

Dioxigenase FTO Dependente de alfa-Cetoglutarato/genética , Obesidade/genética , Sobrepeso/genética , Polimorfismo de Nucleotídeo Único , Idoso , Estudos de Casos e Controles , Diabetes Mellitus Tipo 2/complicações , Feminino , Grécia/epidemiologia , Humanos , Masculino , Obesidade/epidemiologia , Sobrepeso/epidemiologia
Int J Endocrinol ; 2015: 710363, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26078759


Vitamin D deficiency has been associated with both type 2 diabetes mellitus (T2DM) and autoimmune disorders. The association of vitamin D with T2DM and thyroid autoimmunity (TAI) has not been investigated. Thus, we aimed to explore the putative association between T2DM and thyroid autoimmunity (TAI) focusing on the role of 25-hydroxy-vitamin D (25(OH)D). Study population included 264 T2DM patients and 234 controls. To explore the potential association between 25(OH)D and thyroid autoimmunity while controlling for potential confounders-namely, age, gender, body mass index, and presence of T2DM-multivariate logistic regression analyses were undertaken. Patients with T2DM were younger (P < 0.001) and had significantly lower 25(OH)D levels (P < 0.001) and higher anti-TPO titers (P = 0.005). Multivariable logistic regression analyses suggested that T2DM and 25(OH)D levels were significantly associated with the presence of thyroid autoimmunity. In an elderly population of diabetic patients and controls with a high prevalence of vitamin D deficiency/insufficiency, a patient with T2DM was found to be 2.5 times more likely to have thyroid autoimmunity compared to a nondiabetic individual and the higher the serum 25(OH)D levels were, the higher this chance was.

Eur J Endocrinol ; 164(6): 1035-41, 2011 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-21464139


OBJECTIVE: Postprandial suppression of bone resorption is considered one of the main contributors in the circadian rhythm of bone turnover markers. The aim of this study was to investigate this physiological response of bone tissue in diseases that affect bone metabolism. PATIENTS AND METHODS: In this study, 118 patients (45 hypothyroid, 40 hyperthyroid, and 33 ß-thalassemic patients) and 78 healthy individuals matched for age and body mass index were included. An oral glucose test (75 g glucose) was performed after overnight fasting. Serum levels of procollagen type-I N-terminal propeptide (P1NP), ß-C-terminal telopeptide of type I collagen (ß-CTX), and osteocalcin were assayed at 0, 60, and 120 min. RESULTS: Baseline values of bone turnover markers were significantly elevated in hyperthyroid and ß-thalassemic patients but not in hypothyroid patients compared with the control group. After oral glucose, the levels of ß-CTX but not P1NP or osteocalcin were significantly suppressed in all groups (mean change from baseline is 46.9% for ß-CTX, 7.9% for P1NP, and 8% for osteocalcin). The percentage change from baseline for ß-CTX was significantly augmented in hypothyroidism (52 vs 42%, P=0.009). CONCLUSION: The preservation or even augmentation of postprandial suppression of bone resorption in diseases that affect bone metabolism through distinct pathogenetic mechanisms suggests the importance of this physiological response to nutrients for the general homeostasis and functional integrity of the skeleton.

Desenvolvimento Ósseo/fisiologia , Doenças Ósseas Metabólicas/metabolismo , Osso e Ossos/metabolismo , Glucose/farmacologia , Biomarcadores/análise , Doenças Ósseas Metabólicas/diagnóstico , Colágeno Tipo I/metabolismo , Feminino , Teste de Tolerância a Glucose , Homeostase/fisiologia , Humanos , Hipertireoidismo/sangue , Hipotireoidismo/sangue , Masculino , Pessoa de Meia-Idade , Osteocalcina/sangue , Pró-Colágeno/sangue , Talassemia beta/metabolismo