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Emerg Infect Dis ; 26(1): 164-166, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31855545


We investigated the epidemiology of cholera in Taiwan during 2002-2018. Vibrio cholerae sequence type (ST) 75 clone emerged in 2009 and has since become more prevalent than the ST69 clone from a previous pandemic. Closely related ST75 strains have emerged in 4 countries and may now be widespread in Asia.

Artigo em Inglês | MEDLINE | ID: mdl-31383653


Salmonella enterica serovar Goldcoast infection was rare in Taiwan; it was not detected in routine surveillance from 2004 to 2013. This serovar was first identified in 2014, but the frequency of infection remained low until 2017. From 2014 to 2016, all but one isolate was pan-susceptible. S Goldcoast infections abruptly increased in 2018, and all isolates were multidrug-resistant (MDR). All MDR isolates harbored an IncHI2 plasmid, and the majority carried 14 antimicrobial resistance genes, aac(3)-IId, aadA22, aph(3')-Ia, aph(6)-Id, bla TEM-1B, bla CTX-M-55, lnu(F), floR, qnrS13, arr-2, sul2, sul3, tet(A), and dfrA14. S Goldcoast strains recovered in Taiwan and 96 of 99 strains from Germany, the Netherlands, the United Kingdom, and the United States belonged to sequence type 358 (ST358). Whole-genome single-nucleotide polymorphism and core genome multilocus sequence type analyses revealed that all strains of the ST358 clone shared a high degree of genetic relatedness. The present study highlighted that a dramatic increase in S Goldcoast infections followed the emergence of MDR strains and indicated that a genetically closely related S Goldcoast ST358 clone may have widespread significance internationally.

Org Lett ; 21(1): 228-232, 2019 01 04.
Artigo em Inglês | MEDLINE | ID: mdl-30550285


A complementary study including design of mechanistic probes, biochemical assays, model analysis, and liquid chromatography coupled mass spectrometry was conducted to establish the reaction mechanism for a nonheme iron enzyme catalyzed (-)-podophyllotoxin formation. Our results indicate that the originally proposed hydroxylated intermediate is unlikely to be involved in this reaction. Instead, the formation of benzylic radical/carbocation intermediate can be utilized to trigger the C-C bond formation to construct the C-ring of (-)-podophyllotoxin.

Ferroproteínas não Heme/metabolismo , Podofilotoxina/biossíntese , Biocatálise , Ciclização , Conformação Molecular , Oxirredução , Podofilotoxina/química , Estereoisomerismo
Emerg Infect Dis ; 25(1): 144-147, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30561315


In 2011, a Salmonella enterica serovar Anatum clone emerged in Taiwan. During 2016-2017, infections increased dramatically, strongly associated with emergence and spread of multidrug-resistant strains with a plasmid carrying 11 resistance genes, including blaDHA-1. Because these resistant strains infect humans and food animals, control measures are urgently needed.

Antibacterianos/farmacologia , Farmacorresistência Bacteriana Múltipla/genética , Plasmídeos/genética , Infecções por Salmonella/microbiologia , Salmonella enterica/genética , Humanos , Epidemiologia Molecular , Infecções por Salmonella/epidemiologia , Salmonella enterica/efeitos dos fármacos , Taiwan/epidemiologia
Artigo em Inglês | MEDLINE | ID: mdl-29581119


We identified 20 to 22 resistance genes, carried in four incompatibility groups of plasmids, in each of five genetically closely related Salmonella enterica serovar Typhimurium strains recovered from humans, pigs, and chickens. The genes conferred resistance to aminoglycosides, chloramphenicol, sulfonamides, trimethoprim, tetracycline, fluoroquinolones, extended-spectrum cephalosporins and cefoxitin, and azithromycin. This study demonstrates the transmission of multidrug-resistant Salmonella strains among humans and food animals and may be the first identification of mphA in azithromycin-resistant Salmonella strains in Taiwan.

Antibacterianos/farmacologia , Farmacorresistência Bacteriana Múltipla/genética , Plasmídeos/genética , Salmonella typhimurium/efeitos dos fármacos , Salmonella typhimurium/genética , Animais , Azitromicina/farmacologia , Galinhas , Humanos , Testes de Sensibilidade Microbiana , Família Multigênica/genética , Salmonelose Animal , Salmonella typhimurium/isolamento & purificação , Suínos , Taiwan , Sequenciamento Completo do Genoma