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Biol Pharm Bull ; 30(9): 1816-8, 2007 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-17827748


As abnormal behaviors such as jumping and falling from balcony were reported in patients aged 10 to 19 years who administrated oseltamivir phosphate (Tamiflu) for treatment influenza infection, the Ministry of Health, Labor and Welfare in Japan notified that, as a rule, Tamiflu should not be prescribed to patients aged 10 to 19 years. To examine the relationship between Tamiflu and abnormal behaviors, we investigated the effects of Tamiflu and its carboxylic acid metabolite, GS4071, on the central nervous system, that is, on 3 neurotransmitters (dopamine, serotonin, and norepinephrine) in presynapses (inhibition of re-uptake, promotion of release) and postsynapses (guanosine 5'-triphosphate (GTP) gammaS binding), using rat brain synaptosomes. Neither Tamiflu nor GS4071 influenced the re-uptake/release of the 3 monoamines or GTP binding in postsynapses.

Antivirais/farmacologia , Monoaminas Biogênicas/fisiologia , Química Encefálica/efeitos dos fármacos , Oseltamivir/farmacologia , Transmissão Sináptica/efeitos dos fármacos , Inibidores da Captação Adrenérgica/farmacologia , Animais , Dopamina/metabolismo , Inibidores da Captação de Dopamina/farmacologia , Guanosina 5'-O-(3-Tiotrifosfato)/metabolismo , Guanosina Trifosfato/metabolismo , Técnicas In Vitro , Masculino , Norepinefrina/metabolismo , Ratos , Ratos Sprague-Dawley , Serotonina/metabolismo , Inibidores de Captação de Serotonina/farmacologia , Sinaptossomos/efeitos dos fármacos , Sinaptossomos/metabolismo
Shokuhin Eiseigaku Zasshi ; 46(3): 93-8, 2005 Jun.
Artigo em Japonês | MEDLINE | ID: mdl-16042295


Hajikami (ginger pickled in vinegar) is often used as a relish for grilled fish, and it often contains coloring agents. We detected Rose Bengal (R105) and two unknown dyes in some Hajikami by thin layer chromatography. In this study, we tried to characterize these unknown dyes by HPLC with photodiode array detection (PDA-HPLC), liquid chromatography/mass spectrometry (LC/MS) and nuclear magnetic resonance (NMR). PDA-HPLC analysis showed that the spectra of the unknown dyes resembled that of R105, suggesting the molecular structures of these two dyes are similar to that of R105. Furthermore, LC/MS analysis suggested that the these dyes are R105 in which one or two iodines are replaced by hydrogen. Finally, the two dyes were determined by 1H-NMR and 13C-NMR to be 4,5,6,7-tetrachloro-3',6'-dihydroxy-2',7'-diiodo spiro[isobenzofuran- 1 (3H),9'-[9H]xanthen]-3-one disodium salt and 4,5,6,7-tetrachloro-3',6'-dihydroxy-2',7'-diiodo spiro[isobenzofuran-1(3H),9'[9H]xanthen]-3-one disodium salt.

Corantes/química , Análise de Alimentos , Gengibre/química , Cromatografia Líquida de Alta Pressão , Cromatografia Líquida , Corantes/isolamento & purificação , Espectroscopia de Ressonância Magnética , Espectrometria de Massas , Rosa Bengala/análise
Shokuhin Eiseigaku Zasshi ; 43(3): 115-21, 2002 Jun.
Artigo em Japonês | MEDLINE | ID: mdl-12238147


Four kinds of barbaloin (BA)-related compounds (A, B, C, D) in aloe drinks were isolated by using preparative HPLC. LC/MS analyses of these compounds showed the identical quasimolecular ion peak at m/z 833 [M-H]-. The chemical structures were mainly determined by NMR, including 1H-1H two-dimensional correlation spectroscopy (COSY), nuclear Overhauser and exchange spectroscopy (NOESY), heteronuclear multiple quantum coherence (HMQC), and heteronuclear multiple-bond connectivity (HMBC) experiments. They were identified as BA-related compounds A (10R, 10"S), B (10S, 10"S), C (10S, 10"R), and D (10R, 10"R) coupled through a C-10 to C-7' linkage, and newly found in nature. These results suggested that BA is converted to dimers during storage of aloe drinks.

Aloe , Antracenos/química , Cromatografia Líquida de Alta Pressão , Análise de Alimentos , Espectroscopia de Ressonância Magnética
Shokuhin Eiseigaku Zasshi ; 43(3): 122-6, 2002 Jun.
Artigo em Japonês | MEDLINE | ID: mdl-12238148


The contents of barbaloin (BA), isoBA, aloin-dimers A, B, C, D and aloe-emodin (AE) in aloe drinks were investigated. BA and isoBA were detected in 30 of the 31 samples at the levels of 120-570 micrograms/mL and 120-580 micrograms/mL, respectively. Aloin-dimers A, B, C and D were detected in 8 of the 31 samples at the levels of 12-38 micrograms/mL, 13-39 micrograms/mL, 11-36 micrograms/mL and 16-69 micrograms/mL, respectively. AE was detected in all of the 31 samples at the levels of 0.03-1.3 micrograms/mL. When aloe drinks were stored for 4 weeks at 5 degrees C after opening the bottle, decrease of BA and isoBA, and increase of AE and aloin-dimers A, B, C and D were observed in most cases. However, in a few aloe drinks, all of BA, isoBA, aloin-dimers A, B, C, D and AE decreased. In these drinks, the existence of aloin-trimer was elucidated by LC/MS analyses. These data suggested that BA in aloe drinks is converted to the dimer and then to the trimer during storage.

Aloe , Antracenos/isolamento & purificação , Conservação de Alimentos , Antracenos/química , Antraquinonas , Emodina/análise , Análise de Alimentos , Temperatura , Fatores de Tempo