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1.
Int J Mol Sci ; 22(22)2021 Nov 17.
Artigo em Inglês | MEDLINE | ID: mdl-34830275

RESUMO

Peri-implantitis is an unsolved but critical problem with dental implants. It is postulated that creating a seal of gingival soft tissue around the implant neck is key to preventing peri-implantitis. The objective of this study was to determine the effect of UV surface treatment of titanium disks on the adhesion strength and retention time of oral connective tissues as well as on the adherence of mucosal fibroblasts. Titanium disks with a smooth machined surface were prepared and treated with UV light for 15 min. Keratinized mucosal tissue sections (3 × 3 mm) from rat palates were incubated for 24 h on the titanium disks. The adhered tissue sections were then mechanically detached by agitating the culture dishes. The tissue sections remained adherent for significantly longer (15.5 h) on the UV-treated disks than on the untreated control disks (7.5 h). A total of 94% of the tissue sections were adherent for 5 h or longer on the UV-treated disks, whereas only 50% of the sections remained on the control disks for 5 h. The adhesion strength of the tissue sections to the titanium disks, as measured by tensile testing, was six times greater after UV treatment. In the culture studies, mucosal fibroblasts extracted from rat palates were attached to titanium disks by incubating for 24, 48, or 96 h. The number of attached cells was consistently 15-30% greater on the UV-treated disks than on the control disks. The cells were then subjected to mechanical or chemical (trypsinization) detachment. After mechanical detachment, the residual cell rates on the UV-treated surfaces after 24 and 48 h of incubation were 35% and 25% higher, respectively, than those on the control surfaces. The remaining rate after chemical detachment was 74% on the control surface and 88% on the UV-treated surface for the cells cultured for 48 h. These trends were also confirmed in mouse embryonic fibroblasts, with an intense expression of vinculin, a focal adhesion protein, on the UV-treated disks even after detachment. The UV-treated titanium was superhydrophilic, whereas the control titanium was hydrophobic. X-ray photoelectron spectroscopy (XPS) chemical analysis revealed that the amount of carbon at the surface was significantly reduced after UV treatment, while the amount of TiOH molecules was increased. These ex vivo and in vitro results indicate that the UV treatment of titanium increases the adhesion and retention of oral mucosa connective tissue as a result of increased resistance of constituent fibroblasts against exogenous detachment, both mechanically and chemically, as well as UV-induced physicochemical changes of the titanium surface.

2.
PLoS One ; 16(9): e0257208, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34506512

RESUMO

PURPOSE: Previous studies have rarely attempted to test the confounding factors that may affect learning outcomes of the flipped classroom. The purpose of this study was to assess how flipped classrooms affect the acquisition of knowledge in clinical dental education based on multilevel factor analysis. METHOD: The authors conducted a 3-year (2017, 2018, and 2019) randomized controlled trial in a series of introductory prosthodontics courses in dental education. A total of 137 participants were randomly assigned to flipped classroom (n = 70, 51%) or lecture (n = 67, 49%) formats. The flipped group was instructed to self-learn knowledge-based content through online preparation materials, including videos and text, while the lecture group was given text only. Both groups were provided with the same study content and opportunities for different styles of learning. The session attendance rate and number of times the materials were accessed were monitored. Individual and team readiness assurance tests (IRAT/TRAT) were conducted to evaluate knowledge acquisition. A multilevel linear regression analysis was conducted on both instructional styles (flipped vs. lecture) as an intervention factor, and confounding factors that could affect the outcomes were implemented. RESULTS: The average number of online accesses was 2.5 times per session in the flipped group and 1.2 in the lecture group, with a significant difference (p < .05). The average IRAT score was significantly higher in the flipped than in the lecture group (effect size [ES] 0.58, p < .001). The number of online accesses was significantly and positively correlated with IRAT scores (0.6 [0.4, 0.8]). The instructional style was significantly and positively correlated with TRAT scores (coefficient [95% confidence interval]: 4.6 [2.0, 7.3]), but it was not correlated with IRAT (4.3 [-0.45, 9.0]). CONCLUSIONS: The flipped classroom was more effective than the lecture format regarding knowledge acquisition; however, the decisive factor was not the instructional style but the number of individual learning occasions. The employment of the flipped classroom was the decisive factor for team-based learning outcomes.


Assuntos
Educação em Odontologia/métodos , Currículo , Humanos , Aprendizagem Baseada em Problemas/métodos
3.
Biomed Res Int ; 2021: 9987860, 2021.
Artigo em Inglês | MEDLINE | ID: mdl-34195290

RESUMO

Purpose: The potential of UV-mediated photofunctionalization to enhance the resin-based luting agent bonding performance to aged materials was investigated. Methods: Sixty samples of each material were prepared. Yttria-stabilized zirconia (YZr) and Pd-Au alloy (Pd-Au) plates were fabricated and sandblasted. Lithium disilicate glass-ceramic (LDS) was CAD-CAM prepared and ground with #800 SiC paper. Half of the specimens were immersed in machine oil for 24 h to simulate the carbon adsorption. Then, all of the specimens (noncarbon- and carbon-adsorbed) were submitted to UV-mediated photofunctionalization with a 15 W UV-LED (265 nm, 300 mA, 7692 µW/cm2) for 0 (control groups), 5, and 15 min and subjected to contact angle (Ɵ) measurement and bonded using a resin cement (Panavia™ V5, Kuraray Noritake, Japan). The tensile bond strength (TBS) test was performed after 24 h. The Ɵ (°) and TBS (MPa) data were statistically analyzed using two-way ANOVA and Bonferroni correction tests (α = 0.05). Results: In the carbon-adsorbed groups, UV-mediated photofunctionalization for 5 min significantly decreased Ɵ of all materials and increased TBS of YZr, and UV for 15 min significantly increased the TBS of LDS and Pd-Au. In noncarbon-adsorbed groups, UV-photofunctionalization did not significantly change the Ɵ or TBS except YZr specimens UV-photofunctionalized for 15 min. Conclusion: UV-mediated photofunctionalization might have removed the adsorbed hydrocarbon molecules from the materials' surfaces and enhanced bond strengths of Panavia™ V5 to YZr, LDS, and Pd-Au. Additionally, UV-mediated photofunctionalization improved the overall TBS of YZr. Further investigation on the optimum conditions of UV photofunctionalization on indirect restorative materials should be conducted.


Assuntos
Ligas Dentárias , Fotoquímica/métodos , Cimentos de Resina/química , Adsorção , Carbono , Cerâmica , Desenho Assistido por Computador , Colagem Dentária , Porcelana Dentária , Análise do Estresse Dentário , Ouro/química , Humanos , Hidrocarbonetos/química , Teste de Materiais , Paládio/química , Estudos Prospectivos , Propriedades de Superfície , Resistência à Tração , Raios Ultravioleta , Ítrio/química , Zircônio/química
4.
Int J Urol ; 28(10): 1012-1018, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34227174

RESUMO

OBJECTIVE: To evaluate the clinical benefit of tumor contact length as a predictor of pathological extraprostatic extension and biochemical recurrence in patients undergoing prostatectomy. METHODS: A total of 91 patients who underwent 3T multiparametric magnetic resonance imaging before prostatectomy from April 2014 to July 2019 were included. A total of 94 prostate cancer foci were analyzed retrospectively. We evaluated maximum tumor contact length, which was determined to be the maximum value in the three-dimensional directions, as a predictor of pathological extraprostatic extension and biochemical recurrence. RESULTS: A total of 19 lesions (20.2%) had positive pathological extraprostatic extension. Areas under the curves showed maximum tumor contact length to be a significantly better parameter to predict pathological extraprostatic extension than the Prostate Imaging Reporting and Data System (P = 0.002), tumor maximal diameter (P = 0.001), prostate-specific antigen (P = 0.020), Gleason score (P < 0.001), and clinical T stage (P < 0.001). Multivariate analysis showed maximum tumor contact length (P = 0.003) to be an independent risk factor for predicting biochemical recurrence. We classified the patients using preoperative factors (prostate-specific antigen >10, Gleason score >3 + 4 and maximum tumor contact length >10 mm) into three groups: (i) high-risk group (patients having all factors); (ii) intermediate-risk group (patients having two of three factors); and (iii) low-risk group (patients having only one or none of the factors). Kaplan-Meier curves showed that the high-risk group had significantly worse biochemical recurrence than the intermediate-risk group (P = 0.042) and low-risk group (P < 0.001). CONCLUSIONS: Our findings suggest that maximum tumor contact length is an independent predictor of pathological extraprostatic extension and biochemical recurrence. A risk stratification system using prostate-specific antigen, Gleason score and maximum tumor contact length might be useful for preoperative assessment of prostate cancer patients.


Assuntos
Imageamento por Ressonância Magnética Multiparamétrica , Neoplasias da Próstata , Humanos , Imageamento por Ressonância Magnética , Masculino , Valor Preditivo dos Testes , Antígeno Prostático Específico , Prostatectomia , Neoplasias da Próstata/diagnóstico por imagem , Neoplasias da Próstata/cirurgia , Estudos Retrospectivos
5.
J Prosthodont Res ; 2021 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-33504721

RESUMO

PURPOSE: This study comprehensively reviewed the current status of digital workflows in fabricating removable partial dentures (RPDs) using evidence from clinical trials and case reports. STUDY SELECTION: We performed a systematic review of the literature on the materials and fabrication of RPDs using digital technologies published in online databases from 1980 to 2020. We selected eligible articles from the search results, retrieved information on digital RPDs from these, and conducted a qualitative analysis. We report evidence from clinical papers and case reports, digital impression-taking methods, and maxillomandibular relationship (MMR) records. RESULTS: A case report electronically published in 2019 introduced a clasp-retained RPD fabricated via a full-digital workflow without a gypsum definitive cast. Computer-aided design and computer-aided manufacturing of double-crown-retained RPDs with nonmetal materials were described in some case reports. Intraoral scanners were used to obtain digital impressions and MMR records in the fabrication of digital RPDs, which have potential advantages for reducing the number of clinical appointments and simplifying laboratory procedures. Evidence from clinical trials is scarce; a randomized controlled trial reported higher patient satisfaction with digital clasp-retained RPDs than with conventional RPDs. CONCLUSIONS: Full-digital RPDs can be fabricated without a gypsum definitive cast. However, the indication for full-digital RPDs is limited to cases with Kennedy Class III/IV partially edentulous arches with several missing teeth. Challenges in digital impression-taking and MMR recording remain to be solved to extend these indications. More evidence from clinical trials is required to evaluate the efficacy and usefulness of digital R PDs.

6.
J Prosthodont Res ; 2021 Jan 26.
Artigo em Inglês | MEDLINE | ID: mdl-33504722

RESUMO

PURPOSE: This study comprehensively reviewed the current status of the digital workflow of removable partial dentures (RPDs) and summarized information about the fabrication methods and material properties of the dental framework, artificial teeth, and denture base. STUDY SELECTION: We performed a systematic review of the literature published in online databases from January 1980 to April 2020 regarding RPD fabrication and materials used in the related digital technology. We selected eligible articles, retrieved information regarding digital RPDs, and conducted qualitative/quantitative analyses. In this paper, the computer-aided design/computer-aided manufacturing (CAD/CAM) framework, artificial teeth, and denture base materials are reported. RESULTS: A variety of materials, such as cobalt-chromium alloy, titanium, zirconia, and polyether ether ketone, are used for dental CAD/CAM frameworks. The mechanical strength of the metal materials used for the CAD/CAM framework was superior to that of the cast framework. However, the fitness and surface roughness of the framework and clasp fabricated using a selective laser melting (SLM) method were not superior to those obtained via cast fabrication. Most material properties and the surface roughness of poly methyl methacrylate (PMMA) discs used for digital RPDs were superior to those of heat-cured PMMA. CONCLUSIONS: The use of a CAD/CAM framework and PMMA disc for digital RPDs offers numerous advantages over conventional RPDs. However, technical challenges regarding the accuracy and durability of adhesion between the framework and denture base remain to be solved. In digital fabrication, human technical factors influence the quality of the framework.

7.
Hinyokika Kiyo ; 66(8): 251-257, 2020 Aug.
Artigo em Japonês | MEDLINE | ID: mdl-32882121

RESUMO

The clinical outcome of laparoscopic radical prostatectomy (LRP) was retrospectively investigated taking into consideration the surgeon's position during the procedure. The study cohort included 184 consecutive patients who had undergone LRP performed by a single surgeon from February 2013 to July 2018. During the study period,the surgeon stood alternately on either the left or right side of the patient. The D'Amico risk classification was low,intermediate and high in 26 (14.1%),45 (24.5%) and 113 (61.4%) patients,respectively. Mean surgical duration was 203.5 minutes and mean estimated blood loss was 437.6 ml. Nerve sparing (NS) was implemented in 82 (44. 6%) patients. The mean period of having an indwelling urethral catheter was 5. 0 days. Perioperative Clavien-Dindo degree ≥IIIa complications occurred in three (1.6%) patients. Except for cases with presurgical hormonal treatment,surgical margins were positive in 41 (22.3%) patients,among whom 23 (17.4%) had pT2 disease. The 5-year biochemical recurrence-free survival rate was 81.4%,and 84.8% of patients regained urinary continence at 12 months after surgery. Where the surgeon stood during LRP was not associated with significant differences in any parameter. However,the margin positive rate was higher on the side away from where the surgeon stood than the side closer to the surgeon (70.7% vs 29.3%). In conclusion,the position of the surgeon during LRP does not influence the outcome.


Assuntos
Laparoscopia , Neoplasias da Próstata/cirurgia , Cirurgiões , Humanos , Masculino , Prostatectomia , Estudos Retrospectivos , Resultado do Tratamento
8.
J Anesth ; 34(3): 382, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32318816

RESUMO

In the original publication of the article, the value "40-µm thickness" was incorrect in the legend of Figure 4. The correct value is 10-µm thickness.

9.
J Anesth ; 34(3): 373-381, 2020 06.
Artigo em Inglês | MEDLINE | ID: mdl-32189128

RESUMO

PURPOSE: Antiepileptic drugs are used not only for the treatment of epilepsy but also for that of neuropathic pain. However, their action mechanisms have not always been well explained. Stiripentol, an effective antiepileptic drug indicated as a therapeutic for Dravet syndrome, was recently shown to act as an inhibitor of lactate dehydrogenase in astrocytes. In this present study, we examined the effect of stiripentol on neuropathic pain in L5 spinal nerve-transected mice. METHODS: We carried out behavioral tests using calibrated von Frey filaments and the immunohistochemistry of glial fibrillary acidic protein, an astrocyte marker, in L5 spinal nerve-transected mice after intrathecal administration of drugs. RESULTS: Like other anticonvulsants such as gabapentin and carbamazepine, stiripentol alleviated mechanical hyperalgesia induced by L5 spinal nerve transection in a dose-dependent manner, when intrathecally administered to mice 7, 14, and 28 days after L5 spinal nerve transection. Likewise, α-cyano-4-hydroxycinnamic acid, a broad inhibitor of monocarboxylate transporters, diminished mechanical hyperalgesia induced by L5 spinal nerve transection. Simultaneous administration of L-lactate negated the analgesic effect elicited by stiripentol, carbamazepine or α-cyano-4-hydroxycinnamic acid, but not that by gabapentin. None of the anticonvulsants affected the immunoreactivity of glial fibrillary acidic protein. CONCLUSIONS: This present study demonstrated that stiripentol was effective against neuropathic pain and suggested that the astrocyte-neuron lactate shuttle was involved in such pain.


Assuntos
Neuralgia , Nervos Espinhais , Animais , Dioxolanos , Modelos Animais de Doenças , Hiperalgesia/tratamento farmacológico , Camundongos , Neuralgia/tratamento farmacológico , Ratos , Ratos Sprague-Dawley , Medula Espinal
10.
Int J Urol ; 27(2): 140-146, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31733635

RESUMO

OBJECTIVE: To assess the clinical benefits of magnetic resonance imaging/transrectal ultrasound fusion-targeted biopsy for biopsy-naïve Japanese men. METHODS: Between February 2017 and August 2018, 131 biopsy-naïve men who underwent targeted biopsy together with 10-core systematic biopsy at Hiroshima University Hospital were retrospectively investigated. Multiparametric magnetic resonance imaging findings were reported based on Prostate Imaging Reporting and Data System version 2. RESULTS: The overall cancer detection rates per patient were 69.5% in systematic biopsy + targeted biopsy cores, 61.1% in systematic biopsy cores and 61.1% in targeted biopsy cores. The detection rates for clinically significant prostate cancer were 43.5% in targeted biopsy cores and 35.9% in systematic biopsy cores (P = 0.04), whereas the detection rates for clinically insignificant prostate cancer were 17.6% and 25.2% respectively (P = 0.04). Lesions in the peripheral zone were diagnosed more with clinically significant prostate cancer (54.8% vs 20.7%, P < 0.001) and International Society of Urological Pathology grade (3.2 vs 2.7, P = 0.02) than that in the inner gland. Just 4.2% (3/71) of Prostate Imaging Reporting and Data System category 2 and 3 lesions in the middle or base of the inner gland were found to have clinically significant prostate cancer. The cancer detection rate per core was 42.3% in targeted biopsy cores, whereas it was 17.9% in systematic biopsy cores (P < 0.001). CONCLUSIONS: Targeted biopsy is able to improve the diagnostic accuracy of biopsy in detection of clinically significant prostate cancer by reducing the number of clinically insignificant prostate cancer detections compared with 10-core systematic biopsy in biopsy-naïve Japanese men. In addition, the present findings suggest that patients with Prostate Imaging Reporting and Data System category 2 or 3 lesions at the middle or base of the inner gland might avoid biopsies.


Assuntos
Neoplasias da Próstata , Ultrassonografia de Intervenção , Humanos , Biópsia Guiada por Imagem , Japão/epidemiologia , Imageamento por Ressonância Magnética , Masculino , Neoplasias da Próstata/diagnóstico por imagem , Padrões de Referência , Estudos Retrospectivos , Ultrassonografia
11.
Int J Mol Sci ; 20(20)2019 Oct 20.
Artigo em Inglês | MEDLINE | ID: mdl-31635184

RESUMO

Oxidative stress hinders tissue regeneration in cell therapy by inducing apoptosis and dysfunction in transplanted cells. N-acetyl-L-cysteine (NAC) reinforces cellular antioxidant capabilities by increasing a major cellular endogenous antioxidant molecule, glutathione, and promotes osteogenic differentiation. This study investigates the effects of pretreatment of osteoblast-like cells with NAC on oxidative stress-induced apoptosis and dysfunction and bone regeneration in local transplants. Rat femur bone marrow-derived osteoblast-like cells preincubated for 3 h with and without 5 mM NAC were cultured in a NAC-free osteogenic differentiation medium with continuous exposure to 50 µM hydrogen peroxide to induce oxidative stress. NAC preincubation prevented disruption of intracellular redox balance and alleviated apoptosis and negative impact on osteogenic differentiation, even under oxidative stress. Autologous osteoblast-like cells with and without NAC pretreatment in a collagen sponge vehicle were implanted in critical-size defects in rat femurs. In the third week, NAC-pretreated cells yielded complete defect closure with significantly matured lamellar bone tissue in contrast with poor bone healing by cells without pretreatment. Cell-tracking analysis demonstrated direct bone deposition by transplanted cells pretreated with NAC. Pretreatment of osteoblast-like cells with NAC enhances bone regeneration in local transplantation by preventing oxidative stress-induced apoptosis and dysfunction at the transplanted site.


Assuntos
Acetilcisteína/farmacologia , Regeneração Óssea/efeitos dos fármacos , Terapia Baseada em Transplante de Células e Tecidos , Citoproteção , Osteoblastos/citologia , Osteoblastos/transplante , Osteogênese/efeitos dos fármacos , Animais , Sobrevivência Celular , Células Cultivadas , Sequestradores de Radicais Livres/farmacologia , Osteoblastos/efeitos dos fármacos , Oxirredução , Estresse Oxidativo , Ratos , Ratos Sprague-Dawley
12.
J Mech Behav Biomed Mater ; 100: 103393, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31450101

RESUMO

Surface charge is one of the essential physicochemical properties of titanium surfaces for extracellular protein adsorption. Titanium surfaces are generally electronegatively charged at physiological pH. Typical cellular adhesive proteins and cell membranes are also negatively charged. Therefore, there are no direct electric interactions between proteins and titanium surfaces at physiological pH. The objective of this study was to determine how different electrical charges on titanium surfaces affect protein adsorption. Commercially pure grade-2 titanium disks, 19 mm in diameter and 1.5 mm in thickness, having acid-etched micro-roughed surfaces, were prepared. Electropositive charge was supplied by soaking in LiOH solution at concentrations of 0.05, 0.1, 0.25, 0.5, and 1.0 M. After LiOH treatment, X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) were performed. The zeta potential, isoelectric point, and wettability of titanium surfaces were measured. The adsorption levels of proteins, including albumin, laminin, and fibronectin, were evaluated. Osteoblastic cell attachment level was also determined. Incorporation of Li was detected in the oxide layer of titanium without surface morphological modification. The zeta potential was shifted up and the isoelectric point was increased from 3.94 to 5.63 by LiOH treatment. Long-term super-hydrophilicity was also obtained on Li-treated surfaces. The adsorption of albumin and laminin increased with increasing concentration of LiOH treatment solution, whereas fibronectin adsorption was highest upon treatment with 0.25 M. The osteoblastic cell attachment level was shown to be dependent on the amount of fibronectin adsorbed. In conclusion, LiOH treatment enhances biological adhesion on titanium with an increase in surface charge and hydrophilicity. This study suggests that modifying the surface charge provides a direct protein-to-materials interaction and the optimal application of Li should be investigated further.


Assuntos
Lítio/química , Osteoblastos/citologia , Proteínas/química , Titânio/química , Células 3T3 , Adsorção , Albuminas/química , Animais , Adesão Celular , Membrana Celular/metabolismo , Sobrevivência Celular , Fibronectinas/química , Concentração de Íons de Hidrogênio , Íons , Ponto Isoelétrico , Laminina/química , Compostos de Lítio/química , Camundongos , Osteoblastos/metabolismo , Soroalbumina Bovina/química , Propriedades de Superfície
13.
J Biomed Mater Res B Appl Biomater ; 106(1): 73-79, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-27860159

RESUMO

Although titanium-zirconium (Ti-Zr) alloy has been adopted for clinical applications, the ideal proportion of Zr in the alloy has not been identified. In this study, we investigated the biocompatibility of Ti-Zr alloy by evaluating its corrosion resistance to better understand whether there is an optimal range or value of Zr proportion in the alloy. We prepared pure Ti, Ti-30Zr, Ti-50Zr, Ti-70Zr, and pure Zr (mol% of Zr) samples and subjected them to anodic polarization and immersion tests in a lactic acid + sodium chloride (NaCl) solution and artificial saliva. We observed pitting corrosion in the Ti-70Zr and Zr after exposure to both solutions. After the immersion test, we found that pure Ti exhibited the greatest degree of dissolution in the lactic acid + NaCl solution, with the addition of Zr dramatically reducing Ti ion dissolution, with the reduction ultimately exceeding 90% in the case of the Ti-30Zr. Hence, although the localized corrosion resistance under severe conditions was compromised when the Zr content was more than 70%, metal ion release reduced owing to Zr addition and the corresponding formation of a stable passive layer. The results suggest that Ti-30Zr or a Zr proportion of less than 50% would offer an ideal level of corrosion resistance for clinical applications. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 73-79, 2018.


Assuntos
Ligas/química , Ácido Láctico/química , Cloreto de Sódio/química , Corrosão
14.
J Biomed Mater Res B Appl Biomater ; 106(5): 1869-1877, 2018 07.
Artigo em Inglês | MEDLINE | ID: mdl-28926194

RESUMO

Titanium implant surfaces may serve as attachment substrates for various cell types. Since carbon adsorption on titanium is inevitable, this study examined the negative/positive biological reaction of osteoblasts and fibroblasts on carbon-deposited titanium surfaces. Osteogenic MC3T3-E1 and fibrogenic NIH/3T3 cells were separately cultured on titanium disks on which carbon deposition was experimentally regulated to achieve titanium/carbon ratios of 6.5, 0.02, 0.005, and 0. The initial attachment of cells demonstrated that the quantity of attached osteoblasts on Ti/C (0.005) surfaces was 20% lower than that on Ti/C (6.5) surfaces at 4 h of culture. A 40% reduction in cell attachment at 24 h transferring from Ti/C (6.5) to Ti/C (0.005) surfaces highlighted the negative effect of carbon deposition on osteoblast attachment. However, the initial attachment of fibroblasts, which depended on carbon deposition, increased, and the quantity of cells on Ti/C (0.005) surfaces was almost twice that on Ti/C (6.5) surfaces at 4 h of culture. The levels of common differentiation markers of collagen synthesis were also differentially carbon-dependent as total collagen deposition on Ti/C (0.005) decreased by > 30% compared to that on Ti/C (6.5) in osteoblasts after 7 days of culture. In contrast, collagen synthesis in fibroblasts markedly increased as was evident by the increase in carbon deposition. These inverse effects indicate that carbon deposition on a titanium surface would likely be a disadvantage for bone formation, but might represent an effective option for achieving better wound healing and soft tissue sealing around the surface of an implant-neck region. © 2017 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 106B: 1869-1877, 2018.


Assuntos
Carbono/química , Materiais Revestidos Biocompatíveis/química , Fibroblastos/metabolismo , Teste de Materiais , Osteoblastos/metabolismo , Titânio/química , Animais , Adesão Celular , Fibroblastos/citologia , Camundongos , Células NIH 3T3 , Osteoblastos/citologia , Osteogênese
15.
J Vet Med Sci ; 79(9): 1563-1568, 2017 Sep 29.
Artigo em Inglês | MEDLINE | ID: mdl-28781296

RESUMO

Ehlers-Danlos syndrome (EDS) is a group of disorders caused by abnormalities in the extracellular matrix (ECM). Transforming growth factor-ß (TGF-ß) plays a crucial role in formation of the ECM by the SMAD (Sma-and Mad-related protein, mothers against decapentaplegic homolog) pathway. It has been reported that loss of function of zinc transporter ZRT/IRT-like protein 13 (ZIP13) is the cause of the spondylocheiro dysplastic form of EDS (SCD-EDS: OMIM 612350). Our previous study suggested that TGF-ß1 has a relationship with the skin pathological condition in the Zip13-Knockout (KO) mouse, which is a model of SCD-EDS. Thus far, effective treatment based on modern medicine for this syndrome has not yet been established. According to an approach of traditional Chinese medicine, the present study investigates the medicinal effects of Makomo (Zizania latifolia) on certain aspects of SCD-EDS, such as skin morphology and plasma TGF-ß1, in Zip13-KO mice. Increases in densities of collagen fibers and fibrils without a significant change in thickness of the dermal layer were observed in the group of mice fed a Makomo-containing diet. No change in the amount of collagen suggests that Makomo feed does not elevate collagen synthesis, but changes the length of glycosaminoglycan chains and decreases the distance between collagen fibrils. In conclusion, the changes of the skin structure suggest that Makomo can increase the mechanical strength of skin.


Assuntos
Ração Animal , Proteínas de Transporte de Cátions/metabolismo , Dieta , Poaceae , Pele/patologia , Animais , Proteínas de Transporte de Cátions/genética , Colágeno/metabolismo , Síndrome de Ehlers-Danlos/genética , Regulação da Expressão Gênica/efeitos dos fármacos , Camundongos , Camundongos Knockout
18.
Int Cancer Conf J ; 6(4): 154-157, 2017 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31149492

RESUMO

Primary retroperitoneal serous adenocarcinoma (PRSA) is an extremely rare malignancy, with only seven cases having been previously reported. We report a case of PRSA in a 42-year-old woman treated with surgical resection and adjuvant chemotherapy. The histopathological findings of PRSA resemble those of ovarian serous carcinoma, which indicates that a combination of complete surgical resection with adjuvant chemotherapy may be the best treatment option for PRSA.

19.
J Clin Anesth ; 35: 346-349, 2016 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-27871554

RESUMO

STUDY OBJECTIVE: Aspiration pneumonia is a complication of tracheal intubation and mechanical ventilation. We hypothesized that tracheal tubes removed after anesthesia that have an acidic pH may reflect latent regurgitation of gastric fluid. DESIGN: A prospective observational study. SETTING: Operating room. PATIENTS: Tracheal tubes removed from 200 patients (age range, 20-85 years) who had undergone general anesthesia with tracheal intubation and gastric tube placement were examined. MEASUREMENTS: To detect regurgitated gastric fluid on the tubes, we measured pH at 5 different points along the tubes and in the water in which the removed tracheal tubes were immersed. MAIN RESULTS: Of the 200 removed tracheal tubes, 5 had an acidic pH. The tubes were used in patients who were in the prone (1 patients) or head-down (4 patients) position while under anesthesia. The incidence of acidic pH was significantly higher in patients who were in the prone (1/32) or head-down (4/56) position, compared with those in the supine (0/92) or lateral (0/20) position (P<.05). CONCLUSION: Acidic pH was found on about 2.5% of removed tracheal tubes. These tubes were used in patients who were in the prone or head-down position during general anesthesia, although they did not exhibit significant aspiration symptom. Anesthesiologists should be aware of the inherent risk of gastric fluid regurgitation when their patients undergo general anesthesia in these positions.


Assuntos
Anestesia Geral , Intubação Intratraqueal/instrumentação , Pneumonia Aspirativa/diagnóstico , Postura/fisiologia , Respiração Artificial/instrumentação , Feminino , Humanos , Concentração de Íons de Hidrogênio , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos
20.
J Clin Anesth ; 34: 339-43, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27687405

RESUMO

STUDY OBJECTIVE: This study aimed to compare the impact of pillow height on double-lumen tracheal tube (DLT) intubation with McGRATH MAC (McG) in patients undergoing elective surgery. DESIGN: Randomized clinical trial. SETTING: Operating room. PATIENTS: Fifty adult patients scheduled for elective surgery under 1-lung ventilation with an American Society of Anesthesiologists physical status of 1 to 3. INTERVENTIONS: DLT intubation with McG was performed with a high pillow (HP group; 25 patients) or low pillow (LP group; 25 patients) by anesthesiologists. MEASUREMENTS: Intubation time, number of laryngoscopy, number of tracheal intubation attempts to successful intubation, percentage of glottic opening score, and subjective difficulty of laryngoscopy and tube passage through the glottis were assessed. MAIN RESULTS: Intubation time was significantly shorter in the HP group compared with the LP group (HP: 32.1±14.9seconds vs LP: 49.4±11.2seconds, P<.001). The number of laryngoscopy were 1 (HP group, 22 patients; LP group, 17 patients), 2 (HP group, 3 patient; LP group, 7 patients), and 3 (HP group, 0 patient; LP group, 1 patient), with no significant difference between the 2 groups (P=.197). Although the percentage of glottic opening score did not significantly differ between HP and LP groups (HP: 95.6%±6.7% vs LP: 96.0%±12.3%, P=.08), the number of tracheal intubation attempts was significantly lower in the HP group compared with the LP group (P=.009). The visual analog scale score for laryngoscopy did not significantly differ between the 2 groups (P=.54). However, the visual analog scale for tube passage through the glottis was significantly higher in the LP group than in the HP group (P<.001). CONCLUSIONS: Intubation with an HP was associated with a better DLT intubation profile than with an LP with McG, possibly due to smoother tracheal tube progression through the glottis.


Assuntos
Roupas de Cama, Mesa e Banho , Intubação Intratraqueal/métodos , Laringoscopia/métodos , Postura , Idoso , Idoso de 80 Anos ou mais , Procedimentos Cirúrgicos Eletivos/métodos , Desenho de Equipamento , Feminino , Glote , Humanos , Masculino , Pessoa de Meia-Idade , Ventilação Monopulmonar , Estudos Prospectivos
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