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Tree Physiol ; 41(6): 912-926, 2021 06 07.
Artigo em Inglês | MEDLINE | ID: mdl-32348507


Vegetative propagation through somatic embryogenesis is an effective method to produce elite varieties and can be applied as a tool to study the response of plants to different stresses. Several studies show that environmental changes during embryogenesis could determine future plant development. Moreover, we previously reported that physical and chemical conditions during somatic embryogenesis can determine the protein, hormone and metabolite profiles, as well as the micromorphological and ultrastructural organization of embryonal masses and somatic embryos. In this sense, phytohormones are key players throughout the somatic embryogenesis process as well as during numerous stress-adaptation responses. In this work, we first applied different high-temperature regimes (30 °C, 4 weeks; 40 °C, 4 days; 50 °C, 5 min) during induction of Pinus radiata D. Don somatic embryogenesis, together with control temperature (23 °C). Then, the somatic plants regenerated from initiated embryogenic cell lines and cultivated in greenhouse conditions were subjected to drought stress and control treatments to evaluate survival, growth and several physiological traits (relative water content, water potential, photosynthesis, stomatal conductance and transpiration). Based on those preliminary results, even more extreme high-temperature regimes were applied during induction (40 °C, 4 h; 50 °C, 30 min; 60 °C, 5 min) and the corresponding cytokinin profiles of initiated embryonal masses from different lines were analysed. The results showed that the temperature regime during induction had delayed negative effects on drought resilience of somatic plants as indicated by survival, photosynthetic activity and water- use efficiency. However, high temperatures for extended periods of time enhanced subsequent plant growth in well-watered conditions. High-temperature regime treatments induced significant differences in the profile of total cytokinin bases, N6-isopentenyladenine, cis-zeatin riboside and trans-zeatin riboside. We concluded that phytohormones could be potential regulators of stress-response processes during initial steps of somatic embryogenesis and that they may have delayed implications in further developmental processes, determining the performance of the generated plants.

Pinus , Citocininas , Secas , Reguladores de Crescimento de Plantas , Temperatura
Front Plant Sci ; 9: 1898, 2018.
Artigo em Inglês | MEDLINE | ID: mdl-30619440


Somatic embryogenesis (SE) provides us a potent biotechnological tool to manipulate the physical and chemical conditions (water availability) along the process and to study their effect in the final success in terms of quantity of somatic embryos produced. In the last years, our research team has been focused on the study of different aspects of the SE in Pinus spp. One of the main aspects affecting SE is the composition of culture media; in this sense, phytohormones play one of the most crucial roles in this propagation system. Many studies in conifers have shown that different stages of SE and somatic embryo development are correlated with distinct endogenous phytohormone profiles under the stress conditions needed for the process (i.e., cytokinins play a regulatory role in stress signaling, which it is essential for radiata pine SE). Based on this knowledge, the aim of this study was to test the effect of different temperatures (18, 23, and 28°C) and gelling agent concentrations (8, 9, and 10 gL-1) during the maturation stage of Pinus radiata SE in maturation and germination rates. Parallel, phytohormone profile of somatic embryos developed was evaluated. In this sense, the highest gellan gum concentration led to significantly lower water availability. At this gellan gum concentration and 23°C a significantly higher number of somatic embryos was obtained and the overall success of the process increased with respect to other treatments assayed. The somatic embryos produced in these conditions showed the highest concentration of iP-type cytokinins and total ribosides. Although, the different conditions applied during maturation of somatic embryos led to different hormonal profiles, they did not affect the ex vitro survival of the resulting somatic plants, where no significant differences were observed.

Biom J ; 56(5): 786-9, 2014 09.
Artigo em Inglês | MEDLINE | ID: mdl-24652586


This is a discussion of the paper "Overview of object oriented data analysis" by J. Steve Marron and Andrés M. Alonso.

Análise de Dados
Biom J ; 56(3): 383-402, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24301220


Current cancer mortality data are available with a delay of roughly three years due to the administrative procedure necessary to create the registries. Therefore, health agencies rely on forecast cancer deaths. In this context, statistical procedures providing mortality/incidence risk predictions for different regions or health areas are very useful. These predictions are essential for defining priorities for cancer prevention and treatment. The main objective of this work is to evaluate the predictive performance of alternative spatio-temporal models for short-term cancer risk/counts prediction in small areas. All the models analyzed here are presented under a general-mixed model framework, providing a unified structure of presentation and facilitating the use of similar tools for computing the prediction mean squared error. Prostate cancer mortality data are used to illustrate the behavior of the different models in Spanish provinces.

Biometria/métodos , Modelos Estatísticos , Neoplasias da Próstata/mortalidade , Análise Espaço-Temporal , Humanos , Masculino , Análise de Regressão , Medição de Risco , Espanha/epidemiologia
Ann Epidemiol ; 20(12): 906-16, 2010 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-21074105


PURPOSE: In recent decades, a decline in breast cancer mortality has been observed across Europe, and also in Spain. Our objective is to assess the spatio-temporal pattern during the period 1975-2005 by specific age groups (<45, 45-64, ≥65) in the Spanish provinces. METHODS: For each age group, a spatio-temporal P-spline model with a B-spline basis is used to smooth the mortality risks. Smoothing is carried out in three dimensions: longitude, latitude, and time, allowing for a different time evolution of both spatial components. The age-specific decline is calculated as the maximum of the estimated curve in each province. A confidence band for each curve is also provided. RESULTS: For the first age group (<45), the decline in the different provinces is observed between 1986 and 1991. For women aged between 45 to 64 years, the change occurs between 1990 and 1993. For the third age group (≥65), change points range from 1992 to 2000, unlike Malaga and Cadiz where the change has not been observed in the studied period. Northern and some Mediterranean provinces are the areas with higher mortality risks for all the age groups. CONCLUSIONS: A different behavior for breast cancer mortality risks is observed for different provinces among the age specific groups. The decline of mortality is delayed for the oldest age group. Province differences in the implementation of screening programs could explain some of the observed differences.

Neoplasias da Mama/mortalidade , Adulto , Distribuição por Idade , Idoso , Feminino , Geografia , Humanos , Pessoa de Meia-Idade , Modelos Estatísticos , Mortalidade/tendências , Distribuição de Poisson , Espanha/epidemiologia