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1.
Recurso educacional aberto em Português | CVSP - Brasil | ID: cfc-382784

RESUMO

Esta edição do Ligado em Saúde fala sobre fascite plantar, explica por que ela ocorre, quais os fatores que contribuem para seu aparecimento, diz qual o tratamento indicado e orienta exercícios que podem aliviar e prevenir a dor. Para falar sobre o assunto, a apresentadora Marcela Morato recebe o ortopedista, especialista em pé e tornozelo, do Instituto Nacional de Traumatologia e Ortopedia Jamil Haddad (INTO), Vítor Miranda.


Assuntos
Fasciíte Plantar , Doenças do Pé
2.
Mol Neurobiol ; 53(1): 423-435, 2016 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-25465239

RESUMO

SH-SY5Y cells, a neuroblastoma cell line that is a well-established model system to study the initial phases of neuronal differentiation, have been used in studies to elucidate the mechanisms of neuronal differentiation. In the present study, we investigated alterations of gene expression in SH-SY5Y cells during neuronal differentiation mediated by retinoic acid (RA) treatment. We evaluated important pathways involving nuclear factor kappa B (NF-κB), nuclear E2-related factor 2 (Nrf2), glycolytic, and p53 during neuronal differentiation. We also investigated the involvement of reactive oxygen species (ROS) in modulating the gene expression profile of those pathways by antioxidant co-treatment with Trolox®, a hydrophilic analogue of α-tocopherol. We found that RA treatment increases levels of gene expression of NF-κB, glycolytic, and antioxidant pathway genes during neuronal differentiation of SH-SY5Y cells. We also found that ROS production induced by RA treatment in SH-SY5Y cells is involved in gene expression profile alterations, chiefly in NF-κB, and glycolytic pathways. Antioxidant co-treatment with Trolox® reversed the effects mediated by RA NF-κB, and glycolytic pathways gene expression. Interestingly, co-treatment with Trolox® did not reverse the effects in antioxidant gene expression mediated by RA in SH-SY5Y. To confirm neuronal differentiation, we quantified endogenous levels of tyrosine hydroxylase, a recognized marker of neuronal differentiation. Our data suggest that during neuronal differentiation mediated by RA, changes in profile gene expression of important pathways occur. These alterations are in part mediated by ROS production. Therefore, our results reinforce the importance in understanding the mechanism by which RA induces neuronal differentiation in SH-SY5Y cells, principally due this model being commonly used as a neuronal cell model in studies of neuronal pathologies.


Assuntos
Diferenciação Celular/efeitos dos fármacos , Perfilação da Expressão Gênica , Glicólise/genética , Fator 2 Relacionado a NF-E2/genética , NF-kappa B/genética , Neurônios/citologia , Tretinoína/farmacologia , Proteína Supressora de Tumor p53/genética , Diferenciação Celular/genética , Linhagem Celular Tumoral , Regulação da Expressão Gênica/efeitos dos fármacos , Redes Reguladoras de Genes/efeitos dos fármacos , Glicólise/efeitos dos fármacos , Humanos , Fator 2 Relacionado a NF-E2/metabolismo , NF-kappa B/metabolismo , Neurônios/efeitos dos fármacos , Neurônios/metabolismo , Transdução de Sinais/efeitos dos fármacos , Transdução de Sinais/genética , Proteína Supressora de Tumor p53/metabolismo
3.
Ciênc. rural ; 45(1): 92-97, 01/2015. tab
Artigo em Português | LILACS-Express | ID: lil-731075

RESUMO

Avaliou-se o efeito do fornecimento de monensina sódica no desempenho de vacas leiteiras em fase inicial de lactação, cuja alimentação, em sua dieta total no cocho, compôs-se em 81,4% de silagem de milho e 18,6% de concentrado, em que se fez uso de maior proporção de silagem de milho de que concentrado. Foram utilizadas 14 vacas multíparas 7/8 Holandês, aos 20±10 dias pós-parto aproximadamente, com média de 22±0,5kg vaca-1 dia-1 de leite, em delineamento de blocos casualizados, adotando-se o esquema de parcelas subdivididas no tempo, em modelo de medidas repetidas. Os tratamentos nas parcelas foram diferenciados pela presença (200mg animal-1dia-1) ou ausência da monensina na ração e, nas subparcelas, pelos diferentes períodos de avaliação. Não se observaram diferenças no consumo em matéria seca da dieta, conversão alimentar, produção de leite in natura e corrigida e peso vivo nas médias diárias de cada período. No entanto, a produção de sólidos totais foi superior para os tratamentos com o ionóforo, sinalizando que a administração da monensina sódica para vacas no início de lactação modifica o rendimento em produção de leite.


The effect on the performance of early lactation cows when supplying them with sodium monensin was evaluated, whose power in their overall diet in the trough, was composed of 81.4% of corn silage and 18.6% of concentrate in which use was made of a higher proportion of corn silage to concentrate. Fourteen multiparous 7/8 Dutch cows were used, approximately 20±10 days post-labor, with the average weight of 22±0.5kg cow-1 day-1 of lactation, in randomized blocks, adopting the scheme of subdivided portions in time and repetitive measurement model. The treatments in portions were differentiated by the presence (200mg.animal-1day-1) or absence of monensin in the food and, in the sub-portions, different evaluation periods were used. The animals were fed with their total diet in the feeder composed of 81.4% of corn silage and 18.6% of concentrate. No difference was found in the consumption of diet dry matter, alimentary conversion, in natural and corrected production of milk and live weight in the daily averages of each period. However, the production of total solids was superior for treatments with ionophore, signalizing that the administration of sodium monensin in early lactation cows changes the milk production performance.

4.
Ciênc. rural ; 45(1): 92-97, 01/2015. tab
Artigo em Português | LILACS-Express | ID: lil-731098

RESUMO

Avaliou-se o efeito do fornecimento de monensina sódica no desempenho de vacas leiteiras em fase inicial de lactação, cuja alimentação, em sua dieta total no cocho, compôs-se em 81,4% de silagem de milho e 18,6% de concentrado, em que se fez uso de maior proporção de silagem de milho de que concentrado. Foram utilizadas 14 vacas multíparas 7/8 Holandês, aos 20±10 dias pós-parto aproximadamente, com média de 22±0,5kg vaca-1 dia-1 de leite, em delineamento de blocos casualizados, adotando-se o esquema de parcelas subdivididas no tempo, em modelo de medidas repetidas. Os tratamentos nas parcelas foram diferenciados pela presença (200mg animal-1dia-1) ou ausência da monensina na ração e, nas subparcelas, pelos diferentes períodos de avaliação. Não se observaram diferenças no consumo em matéria seca da dieta, conversão alimentar, produção de leite in natura e corrigida e peso vivo nas médias diárias de cada período. No entanto, a produção de sólidos totais foi superior para os tratamentos com o ionóforo, sinalizando que a administração da monensina sódica para vacas no início de lactação modifica o rendimento em produção de leite.


The effect on the performance of early lactation cows when supplying them with sodium monensin was evaluated, whose power in their overall diet in the trough, was composed of 81.4% of corn silage and 18.6% of concentrate in which use was made of a higher proportion of corn silage to concentrate. Fourteen multiparous 7/8 Dutch cows were used, approximately 20±10 days post-labor, with the average weight of 22±0.5kg cow-1 day-1 of lactation, in randomized blocks, adopting the scheme of subdivided portions in time and repetitive measurement model. The treatments in portions were differentiated by the presence (200mg.animal-1day-1) or absence of monensin in the food and, in the sub-portions, different evaluation periods were used. The animals were fed with their total diet in the feeder composed of 81.4% of corn silage and 18.6% of concentrate. No difference was found in the consumption of diet dry matter, alimentary conversion, in natural and corrected production of milk and live weight in the daily averages of each period. However, the production of total solids was superior for treatments with ionophore, signalizing that the administration of sodium monensin in early lactation cows changes the milk production performance.

5.
Anticancer Agents Med Chem ; 14(8): 1128-35, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25115457

RESUMO

Cellular and molecular mechanisms related to lung cancer have been extensively studied in recent years, but the availability of effective treatments is still scarce. Hecogenin acetate, a natural saponin presenting a wide spectrum of reported pharmacological activities, has been previously evaluated for its anticancer/antiproliferative activity in some in vivo and in vitro models. Here, we investigated the effects of hecogenin acetate in a human lung cancer cell line. A549 non-small lung cancer cells were exposed to different concentrations of hecogenin acetate and reactive species production, ERK1/2 activation, matrix metalloproteinase expression, cell cycle arrest and cell senescence parameters were evaluated. Hecogenin acetate significantly inhibited increase in intracellular reactive species production induced by H2O2. In addition, hecogenin acetate blocked ERK1/2 phosphorylation and inhibited the increase in MMP-2 caused by H2O2. Treatment with hecogenin acetate induced G0/G1-phase arrest at two concentrations (75 and 100 µM, 74% and 84.3% respectively), and increased the staining of senescence-associated ß -galactosidase positive cells. These data indicate that hecogenin acetate is able to exert anti-cancer effects by modulating reactive species production, inducing cell cycle arrest and senescence and also modulating ERK1/2 phosphorylation and MMP-2 production.


Assuntos
Antineoplásicos/farmacologia , Carcinoma Pulmonar de Células não Pequenas/patologia , Senescência Celular/efeitos dos fármacos , Pontos de Checagem da Fase G1 do Ciclo Celular/efeitos dos fármacos , Neoplasias Pulmonares/patologia , Espécies Reativas de Oxigênio/metabolismo , Compostos de Espiro/farmacologia , Esteroides/farmacologia , Carcinoma Pulmonar de Células não Pequenas/metabolismo , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Sobrevivência Celular/efeitos dos fármacos , MAP Quinases Reguladas por Sinal Extracelular/metabolismo , Humanos , Neoplasias Pulmonares/metabolismo , Metaloproteinase 2 da Matriz/metabolismo , Invasividade Neoplásica/patologia , Estresse Oxidativo/efeitos dos fármacos , Fosforilação
6.
Surg Endosc ; 23(11): 2550-5, 2009 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-19343424

RESUMO

BACKGROUND: Clinical applications of transvaginal Natural Orifice Transluminal Endoscopic Surgery (NOTES) are still limited in the literature, mostly case reports performed by hybrid procedures with laparoscopic assistance. Avoiding complications from incisions is the main goal for natural orifice surgery. This study reports on a technique developed at our institution that uses two endoscopes inserted into the vagina to perform a Totally NOTES (T-NOTES) transvaginal cholecystectomy, and describes preliminary results. METHODS: IRB approval was obtained at the institution for transvaginal NOTES clinical trials, and informed consent was obtained. The technique of T-NOTES transvaginal cholecystectomy was clinically applied in four female patients with symptomatic cholelithiasis, and data were prospectively documented. Transvaginal NOTES access was obtained by direct vaginal incision, and two endoscopes were simultaneously introduced in the abdominal cavity. Dissection was accomplished with available endoscopic instruments. Ligation of cystic duct and artery was performed using endoscopic clips. Vaginal closure was achieved using the direct-vision sutured technique. RESULTS: The technique was successfully performed in the four patients. Insufflation and spatial orientation was of good quality. Mean operative time was 210 min. There were no complications during the 30-day follow-up. Postoperative course was uneventful, and patients were released from the hospital on the first postoperative day. CONCLUSION: Tranvaginal T-NOTES using two endoscopes provides a feasible method for natural orifice cholecystectomy using available technology. Large-series studies are needed to evaluate the results with respect to safety of the approach.


Assuntos
Colecistectomia Laparoscópica/métodos , Colelitíase/cirurgia , Procedimentos Cirúrgicos Minimamente Invasivos/métodos , Adulto , Brasil , Colecistectomia Laparoscópica/efeitos adversos , Colelitíase/diagnóstico , Estudos de Coortes , Colonoscópios , Feminino , Seguimentos , Gastroscópios , Humanos , Pessoa de Meia-Idade , Procedimentos Cirúrgicos Minimamente Invasivos/efeitos adversos , Medição de Risco , Fatores de Tempo , Resultado do Tratamento , Vagina/cirurgia
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