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1.
Clin Oral Investig ; 2020 Jul 09.
Artigo em Inglês | MEDLINE | ID: mdl-32648061

RESUMO

OBJECTIVE: The present study aimed to evaluate if genetic variants in PAX9, MSX1, TGFα, FGF3, FGF10, FGF13, GLI2 and GLI3 are involved in TS of permanent teeth. MATERIALS AND METHODS: Pretreatment dental records from orthodontic patients were assessed prior to recruitment. Patients with tooth agenesis and congenital anomalies (including oral cleft) and/or syndromes were excluded. Dental casts were used to measure the maximum crown dimensions of all fully erupted permanent teeth except second and third molars in mesiodistal direction. Teeth with caries, occlusal wear, mesiodistal restorations, and obvious deformities were not evaluated. Genomic DNA samples were used for genotyping. The allelic discrimination of 13 genetic variants was performed. The associations between TS and genotype were analyzed by linear regression, adjusted by gender at a significance level of p ≤ 0.05. RESULTS: Genetic polymorphisms in the tooth agenesis-related genes studied here were associated with increased and decreased TS, in both maxilla and mandible (p < 0.05). CONCLUSION: This study reported associations of novel tooth agenesis-related gene variants with permanent tooth size variations. CLINICAL RELEVANCE: The presence of some genetic variants could allow the prediction of permanent tooth size.

2.
Braz Oral Res ; 34: e055, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32578798

RESUMO

This study was performed to evaluate the interplay between dental caries, nutritional status, and genetic polymorphisms in TAS1R1 and TAS1R2 (taste receptor, type 1, member 1 and 2) in preschool children and pre-adolescents. We included 525 subjects (306 preschool children and 219 pre-adolescents). Parents/caregivers answered a self-administered questionnaire about their children's systemic health, characteristics, oral hygiene habits, and diet. Clinical examination was performed to evaluate dental caries and nutritional status. Saliva samples were collected for DNA extraction. The genotyping of rs17492553 ( TAS1R1 ), rs3935570, and rs4920566 ( TAS1R2 ) polymorphisms was performed using real-time PCR with Taqman Genotyping Master Mix and SNP assay. Both univariate and multivariate Poisson regression analyses with robust variance were used for the data analysis. In preschool children, consumption of sweets between meals increased the prevalence of dental caries by 85% (PR c = 1.85; 95%CI 1.39-2.46; p < 0.001), whereas in pre-adolescents, this prevalence increased by 34% (PR a = 1.34; 95%CI 1.11-1.62; p = 0.002), regardless of genetic polymorphisms . Moreover, individuals carrying at least one allele C in rs17492553 presented 23% more prevalence of dental caries (PR a = 1.23; 95%CI 1.02-1.49 p = 0.030). Nutritional status was not associated with dental caries, neither with genetic polymorphisms . Consumption of sweets between meals increased the prevalence of dental caries. In pre-adolescents, rs17492553 genetic polymorphism in TAS1R1 was associated with dental caries.


Assuntos
Cárie Dentária/genética , Estado Nutricional/genética , Polimorfismo Genético , Receptores Acoplados a Proteínas-G/genética , Adolescente , Brasil/epidemiologia , Criança , Índice CPO , Cárie Dentária/epidemiologia , Feminino , Humanos , Masculino , Prevalência , Reação em Cadeia da Polimerase em Tempo Real , Análise de Regressão , Fatores de Risco , Inquéritos e Questionários , Paladar/genética
3.
Preprint em Inglês | SciELO Preprints | ID: pps-779

RESUMO

Aim: To describe the management of dental education in three dental schools during the COVID-19 crisis. Methods: Adopted strategies in the Federal University of Paraíba (UFPB), Brazil, University of Pittsburgh (UP), USA, and Griffith University (GU), Australia were detailed. Results: In the UFPB, all on-site teaching was suspended, and resources for distance learning set up as a supplementary semester to be available as face to face classes later. A protocol for clinical care followed safety measures recommended by Brazilian official health institutions. The adequacy of the physical structure, human resources, and personal protective equipment (PPE) acquisition for the return to clinical activities are currently under discussion. In the UP, learning activities were shifted to virtual teaching using lecture recordings and live sections. All elective patients care was postponed. Urgent dental cases were discussed via teledentistry. The physical layout of the dental clinics and pre-clinical laboratories were changed, allowing a safe distance between students. In the GU, all clinical and pre-clinical activities were cancelled, and theoretical activities were maintained online for all students. Several clinically based scenarios where created and delivered in the format of online problem-based learning. The reception area was redesigned, ensuring social distancing. Safety measures follow the Australia Dental Association. Conclusions: Dealing with dental education depends on the stage of the epidemic and the characteristics of each country.

4.
J Appl Oral Sci ; 28: e20190628, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32520078

RESUMO

OBJECTIVE: The study of dental development in individuals born with cleft lip and palate (CLP) serves to determine when orthodontic intervention should start. To evaluate the permanent second molar development in children born with cleft lip and palate according to Demirjian's and Nolla's methods. METHODOLOGY: Out of a total of 513 digital panoramic radiographs, 113 pairs of children aged 3 to 16 years were selected. The exams were from children born with or without cleft lip and palate, of the same sex, with an age difference of up to 30 days. The images were analyzed by three examiners and reliability was checked through intra-examiner agreement by the Kappa test. The data were analyzed by Wilcoxon's and Mann-Whitney tests according to each dataset. RESULTS: The findings indicated delayed development of the permanent second molars in children with CLP (P<0.001). The development of the right permanent second molar was delayed compared to the left molar in children with CLP. Moreover, mandibular teeth showed significantly earlier development than maxillary teeth in both the case and control groups. There was no significant difference in the development of permanent second molars between sexes. CONCLUSION: Children with CLP presented delay in the development of permanent second molars.


Assuntos
Fenda Labial/fisiopatologia , Fissura Palatina/fisiopatologia , Dentição Permanente , Dente Molar/crescimento & desenvolvimento , Adolescente , Estudos de Casos e Controles , Criança , Pré-Escolar , Feminino , Humanos , Masculino , Dente Molar/diagnóstico por imagem , Valores de Referência , Fatores Sexuais , Estatísticas não Paramétricas
5.
Crit Rev Toxicol ; 50(4): 348-358, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32343161

RESUMO

Urine has been a biological matrix of choice for drug screening, but recent advances in technology and the introduction of commercial oral fluid assays have effectively established oral fluid as a viable alternative matrix. This systematic review aimed to evaluate the sensitivity of oral fluid in detecting some illicit drugs compared to urine, and to compare the initial and final detection times of these drugs in both fluids. The electronic search in MEDLINE, Cochrane Library, Scopus, and Web of Science was carried out covering studies published from January 2003 and June 2019, in order to find all valid studies that detected drugs in oral fluid and urine in the same patient. Studies about illicit drugs, such as tetrahydrocannabinol, cocaine, amphetamines and illicit opioids, with fluids collection at the same day, controlled drug administration during the study, reported administration interval and time of collection were favored. Out of 2598 studies identified by electronic search, 7 studies were selected for qualitative analysis. Five were clinical trials and 2 were crossover trials. In total, 74 patients aged 20-52 years underwent a diagnostic examination (4 studies with tetrahydrocannabinol, 1 with methamphetamine, and 2 with cocaine) after drug administration. Illicit drug detection in oral fluid is similar to urine but oral fluid has a strong potential for the immediate detection of recent marijuana use compared to urine. In relation to cocaine and methamphetamine, the largest drugs detection window is obtained through urine analysis. Oral fluids cannot replace urine for most of the purposes of drug testing.

6.
Prog Orthod ; 21(1): 9, 2020 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-32249341

RESUMO

BACKGROUND: The aim of the present study was to assess if genetic polymorphisms in tooth agenesis (TA)-related genes are associated with craniofacial morphological patterns. METHODS: This cross-sectional, multi-center, genetic study evaluated 594 orthodontic Brazilians patients. The presence or absence of TA was determined by analysis of panoramic radiography. The patients were classified according to their skeletal malocclusion and facial growth pattern by means of digital cephalometric analysis. Genomic DNA was extracted from squamous epithelial cells of buccal mucosa and genetic polymorphisms in MSX1 (rs1042484), PAX9 (rs8004560), TGF-α (rs2902345), FGF3 (rs1893047), FGF10 (rs900379), and FGF13 (rs12838463, rs5931572, and rs5974804) were genotyped by polymerase chain reaction using TaqMan chemistry and end-point analysis. RESULTS: Genotypes (p = 0.038) and allele (p = 0.037) distributions for the FGF3 rs1893047 were significantly different according to the skeletal malocclusion. Carrying at least one G allele increased in more than two times the chance of presenting skeletal class III malocclusion (OR = 2.21, CI 95% = 1.14-4.32; p = 0.017). There was no association between another skeletal craniofacial pattern and some polymorphism assessed in the present study. CONCLUSION: Our results suggest that the genetic polymorphism rs1893047 in FGF3 might contribute to variations in the craniofacial sagittal pattern.


Assuntos
Anodontia , Má Oclusão , Brasil , Cefalometria , Estudos Transversais , Genótipo , Humanos
7.
J Craniofac Surg ; 2020 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-32209932

RESUMO

BACKGROUND: The oronasal fistula in cleft patients is one of the complications that can be found after primary surgeries due to a failure of healing of the surgical repair that causes the communication between the oral and nasal cavities. A number of procedures can be implemented to correct the fistula and it is not clear if a particular technique is the best to be recommended. OBJECTIVE: This study aims to systematically analyze the scientific evidence regarding the treatment of oronasal fistulas located in the lingual-alveolar and labial-alveolar regions in patients with cleft lip and palate who have undergone primary surgeries. MATERIAL AND METHODS: A bibliographic search of articles published until September 2018 without restricted year and language of publication, in PubMed (Medline), Scopus, Cochrane, Web of science, and BVS databases. The MeSHterms "Fistula," "Oral Fistula," and "Cleft Lip" were used, which were related to each other and with other keywords related to the subject of the review through the "OR" and "AND" operators. The quality of the publications was evaluated according to the guidelines of the Methodological Index for Nonrandomized Studies. RESULTS: After applying the eligibility criteria, a total of 18 articles were selected for the extraction of data and qualitative analysis. CONCLUSION: All publications analyzed in this review reported the fistula treatment at the same surgical time as the bone graft, independently of the donor area, the type of cleft treated and the patient's age at operation. There was no consensus among the studies on the best treatment type for oronasal fistulas located in the alveolar region, and further comparative studies between the existing techniques will be necessary to address this question.

8.
Caries Res ; : 1-11, 2020 Feb 11.
Artigo em Inglês | MEDLINE | ID: mdl-32045909

RESUMO

Dental erosive wear is a multifactorial condition of high prevalence. Nowadays, there is an emphasis on discovering individual genetic predisposition for the development of this condition. Aquaporins (AQPs) are water channel proteins expressed in salivary glands, as well as during tooth development. They are involved in salivary secretion and composition and linked to physiological protection of the oral cavity. The aim of this study was to explore the relationship between different dental erosive wear phenotypes, AQP genes, and selected environmental factors. Data from 705 dental patients were used to investigate the association between dental erosive wear phenotypes and AQPs' single-nucleotide variants. Phenotypes were further analyzed considering diet and oral hygiene data, using logistic regression analysis, as implemented in PLINK, with the assumption that dental erosive wear is a complex gene-environment model. Associations were found between severe erosive tooth wear and rs2878771 (AQP2) for the genotypic (p = 0.02) and dominant (p = 0.03) models, and rs3736309 (AQP5) for the allelic model (p = 0.02). Logistic regression analyses, after implementing the Bonferroni correction, showed that several significant associations were present when covariates were included, suggesting that a strong environmental component is present. Our results show that dental erosive wear establishes under a gene-environmental complex model.

9.
Caries Res ; 54(1): 2-6, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-31610535

RESUMO

Our understanding of erosive tooth wear and its contributing factors has evolved considerably over the last decades. New terms have been continuously introduced, which frequently describe the same aspects of this condition, whereas other terms are being used inappropriately. This has led to unnecessary confusion and miscommunication between patients, professionals, and researchers. A group of 15 experts, selected by the European Organization for Caries Research (ORCA) and the Cariology Research Group of the International Association for Dental Research (IADR), participated in a 2-day workshop to define the most commonly used terms in erosive tooth wear. A modified Delphi method was utilized to reach consensus. At least 80% agreement was achieved for all terms discussed and their definitions related to clinical conditions and processes, basic concepts, diagnosis, risk, and prevention and management of erosive tooth wear. Use of the terms agreed on will provide a better understanding of erosive tooth wear and intends to enable improved communication on this topic.

10.
Artigo em Inglês | LILACS-Express | ID: biblio-1056877

RESUMO

Abstract Objective: To define the prevalence of Molar Incisor Hypomineralization (MIH) in Brazil since the reports ranged from 2.5% to 40.2%. Material and Methods: We studied 407 children from 7 to 14 years of age. MIH was measured using the European Academy of Paediatric Dentistry criteria of 2003. Clinical data were collected by a calibrated dentist (Kappa=0.88) and included affected teeth and degree of MIH severity (mild/severe). Mild MIH cases were considered when the tooth presented demarcated opacity ≥ 1.0mm, without any loss of structure. While severe cases were defined by teeth in which loss of structure was present, or past or current lesion that required treatment, or presence of atypical restorations. In addition, published data (nine studies) reporting MIH in Brazilians were identified, and the heterogeneity of these studies was tested (I2 index/ p≤0.01). Results: In the original sample studied, the majority of patients were males (55.3%; n = 225), with an average age of 10.1 years (± 2.1 years). The prevalence of MIH in this group was 14.5% (59 affected in 407), and most of the affected teeth had a mild degree of alteration (77.4% or 202 in 261 teeth). Conclusion: A meta-analysis including nine published reports, and our original data showed that MIH prevalence in Brazil is 13.48 (95% CI, 8.66% -18.31%).

11.
J Craniofac Surg ; 30(7): 2082-2084, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31574782

RESUMO

BACKGROUND: To evaluate whether genetic polymorphisms in FGF3, FGF10, and FGF13 are associated with temporomandibular disorders (TMD) in patients that presented dentofacial deformities requiring orthognathic surgery. MATERIAL AND METHODS: The sample comprised a total of 113 patients of both sexes. The diagnosis of TMD was performed before orthognathic surgery between Research Diagnostic Criteria for Temporomandibular Disorders (RDC-TMD). According to the TMD assessment, the patients were divided into 3 major groups: myofascial pain, articular disc displacements and other TMD conditions (arthralgia, arthritis, and arthrosis). Genomic DNA was collected from saliva samples and genetic polymorphisms in FGF3 (rs1893047 and rs7932320), FGF10 (rs900379) and FGF13 (rs5931572 and rs5974804) were analyzed by real-time polymerase chain reactions. The association between the TMD conditions and the genetic polymorphisms assessed were analyzed by Poisson Regression. The model was calculated on bivariate and adjusted by sex. The established alpha was 5%. Data were analyzed by using SPSS software (IBM, Armonk, NY). RESULTS: The genetic polymorphisms rs7932320 in FGF3 (P < 0.001) and rs900379 in FGF10 (P < 0.05) were associated with the presence of muscle disorder. The genetic polymorphisms rs1893047 in FGF3, rs900379 in FGF10, and rs5974804 and rs5931572 in FGF13, were associated with the presence of disk displacement (P < 0.05). The genetic polymorphisms rs1893047 and rs7932320 in FGF3, rs900379 in FGF10, and rs900379 in FGF10 were associated with other TMD conditions (P < 0.05). CONCLUSION: Genetic polymorphisms in FGF3, FGF10, and FGF13 genes were associated with temporomandibular disorders in a population with dentofacial deformities.


Assuntos
Fator 10 de Crescimento de Fibroblastos/genética , Fator 3 de Crescimento de Fibroblastos/genética , Fatores de Crescimento de Fibroblastos/genética , Polimorfismo Genético , Transtornos da Articulação Temporomandibular/genética , Adolescente , Adulto , Artralgia , Artrite , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Cirurgia Ortognática , Procedimentos Cirúrgicos Ortognáticos , Osteoartrite/diagnóstico , Inquéritos e Questionários , Transtornos da Articulação Temporomandibular/diagnóstico , Transtornos da Articulação Temporomandibular/cirurgia , Adulto Jovem
12.
Cranio ; : 1-11, 2019 Jul 02.
Artigo em Inglês | MEDLINE | ID: mdl-31264537

RESUMO

Objective: To evaluate the association between polymorphisms in genes and comorbid presence of arthralgias and TMD. Methods: This is a case-control study. The groups formed were individuals with chronic arthralgia and 1) myofascial pain (n = 42); 2) articular (n = 16); 3) multiple diagnoses (n = 69); 4) with TMD and without some other arthralgia (n = 16); 5) without TMD but with pain in other joints (n = 82); and 6) a control group (n = 72). SNPs in COMT, ADRB2, and HTR1A genes were investigated. Results: The CT genotype for the COMT (rs9332377) gene was associated with the absence of myofascial pain (p = .05). In the ADRB2 (rs1042713) gene, the AA genotype was associated with the absence of myofascial pain (p = .03). Discussion: This study supports the hypothesis that alterations in the COMT, ADRB2, and HTR1A genes influence the presence of chronic pain and TMD.

13.
Caries Res ; 53(4): 482-488, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30943522

RESUMO

Molar-incisor hypomineralization (MIH) is a condition that is defined based on its peculiar clinical presentation. Original reports on the etiology of the condition and possible risk factors were inconclusive, and we refuted the original suggestion that MIH is an idiopathic condition and suggested that MIH has complex inheritance and is due to the interaction of more than one gene and the environment. Our group was the first to suggest MIH has a genetic component that involves genetic variation in genes expressed during dental enamel formation. Later we expanded this work to include genes related to the immune response. In this report, we provide a rationale to explain the variation seen in the clinical presentation of MIH, which can affect just one molar out of the four or just a portion of a particular molar.

14.
Caries Res ; 53(4): 441-446, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30759432

RESUMO

The aim of this study was to investigate the association between genetic polymorphisms in MMP8, MMP13, and MMP20 with caries experience and developmental defects of enamel (DDE) in children from the Amazon region of Brazil. Den tal caries and DDE data were collected through clinical examination from 216 children. Genomic DNA was extracted from saliva, and genotyping of selected polymorphisms in MMP8 (rs17099443 and rs3765620), MMP13 (rs478927 and rs2252070), and MMP20 (rs1784418) was performed using TaqMan chemistry and endpoint analysis. χ2 or Fisher's exact tests were used to compare allele and genotype distributions between children with caries experience and caries-free children and between DDE-affected and -unaffected children with an established alpha of 5%. The polymorphism rs478927 in MMP13 was associated with caries experience and DDE (p < 0.05). The analysis performed comparing children with both conditions (caries experience plus DDE) and children with neither of the conditions (caries-free chil dren without DDE) demonstrated that children carrying the MMP13 rs478927 TT genotype were more likely to have concomitant occurrence of these two conditions (OR = 5.8, 95% CI 2.1-15.8; p = 0.0003). In conclusion, the genetic polymorphism rs478927 in MMP13 was associated with caries experience and DDE.

15.
J Oral Rehabil ; 46(7): 597-604, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-30811655

RESUMO

BACKGROUND: Temporomandibular disorder (TMD) is a multifactorial condition involving environmental, psychological and genetic factors. OBJECTIVE: The aim of this case-control study was to evaluate the influence of genetic polymorphisms in 5HTT and COMT on TMD and anxiety in adolescents. METHODS: TMD was diagnosed and classified according to the RDC/TMD criteria. For case group, the following TMD categories were used: myofascial pain, disc displacement, arthralgia and painful TMD (myofascial and arthralgia). Anxiety levels were assessed according to the State-Trait Anxiety Inventory. Genomic DNA was extracted, and genetic polymorphisms were genotyped by TaqMan chemistry and endpoint analysis. Logistic multivariate regression was used to analyse the associations between TMD types and genotypes, anxiety level and genotypes, using an adjusted odds ratio (ORa ; CI 95%) that considered the gender. RESULTS: In 5HTT, the rs1042173 was associated with painful TMD (arthralgia and myofascial pain) (ORc  = 1.97; CI 95%: 1.02-3.77; P = 0.04). Polymorphisms in COMT rs4818 were significantly associated with myofascial pain (ORc  = 2.15; CI 95%: 1.08-4.29; P = 0.02) and were borderline for painful TMD (ORc  = 1.85; CI 95%: 0.97-3.51; P = 0.06) and disc displacement (ORc  = 2.42; CI 95%: 1.00-5.87; P = 0.05). The rs6269 was borderline for myofascial pain (ORc  = 1.82; CI 95%: 0.92-3.59; P = 0.08) and disc displacement (ORc  = 2.38; CI 95% 0.95-5.97; P = 0.06) and also was associated with anxiety (ORa  = 2.34; CI 95% 1.04-5.25; P = 0.03). CONCLUSION: Polymorphisms in 5HTT and COMT are associated with TMD in adolescents. Moreover, polymorphism in COMT is associated with anxiety in adolescents.


Assuntos
Catecol O-Metiltransferase , Transtornos da Articulação Temporomandibular , Adolescente , Ansiedade , Artralgia , Estudos de Casos e Controles , Humanos , Polimorfismo Genético , Proteínas da Membrana Plasmática de Transporte de Serotonina
16.
Caries Res ; 53(2): 217-227, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-30130760

RESUMO

Ameloblasts are sensitive cells whose metabolism and function may be affected by inflammatory stimuli. The aim of this study was to evaluate the possible association between polymorphisms in immune response-related genes and molar-incisor hypomineralization (MIH), and their interaction with polymorphisms in amelogenesis-related genes. DNA samples were obtained from 101 nuclear families that had at least 1 MIH-affected child. Eleven single-nucleotide polymorphisms (SNPs) were investigated in immune response genes using TaqMan® technology allele-specific probes. A transmission disequilibrium test was performed to verify overtransmission of alleles in all MIH families, as well as in families only with mild or severe MIH-affected children. Gene-gene interactions between the immune-related and amelogenesis-related polymorphisms were analyzed by determining whether alleles of those genes were transmitted from heterozygous parents more often in association than individually with MIH-affected children. In severe cases of MIH, significant results were observed for rs10733708 (TGFBR1, OR = 3.5, 95% CI = 1.1-10.6). Statistical evidence for gene-gene interactions between rs6654939 (AMELX) and the SNPs rs2070874 (IL4), rs2275913 (IL17A), rs1800872 (IL10), rs1800587 (IL1A), and rs3771300 (STAT1) was observed. The rs2070874 SNP (IL4) was also significantly overtransmitted from heterozygous parents with the rs7526319 (TUFT1) and the rs2355767 (BMP2) SNPs, suggesting a synergistic effect of the transmission of these alleles with susceptibility to MIH. This family-based study demonstrated an association between variation in TGFBR1 and MIH. Moreover, the polymorphisms in immune response and amelogenesis genes may have an additive effect on the risk of developing MIH.


Assuntos
Amelogênese , Hipoplasia do Esmalte Dentário , Criança , Humanos , Incisivo , Dente Molar , Polimorfismo de Nucleotídeo Único , Prevalência
17.
Cranio ; 37(3): 153-158, 2019 May.
Artigo em Inglês | MEDLINE | ID: mdl-29216797

RESUMO

Objective Compare the anatomical dimensions of mandibular condyles with the same CBCT measurements. Methods Four landmarks were identified on the condyles of dried skulls and are measured with their corresponding areas in CBCT images: Right Width of Condyle (RW), Left Width of Condyle (LW), Right Length of Condyle (RL), and Left Length of Condyle (LL). Results With respect to direct measurements on the dry skulls, statistically significant differences were found between sexes (p < 0.001). When the real values were compared with the CBCT, it was observed that the bias depends on the magnitude of the measurement. It means that there is an underestimation for smaller values and an overestimation for larger values. Conclusion CBCT is reliable imaging modality, but when compared real measures of condyle length are more reliable then width.


Assuntos
Tomografia Computadorizada de Feixe Cônico , Côndilo Mandibular , Imageamento Tridimensional , Crânio
18.
Clin Oral Investig ; 23(6): 2583-2591, 2019 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-30315423

RESUMO

OBJECTIVE: The aim of this study was to evaluate microbiological changes, oral soft tissue toxicity, and caries-preventive effect of an experimental titanium tetrafluoride (TiF4) varnish compared with a commercially available fluoride varnish (NaF), using in situ and in vivo models. MATERIALS AND METHODS: The treatment groups were the following: TiF4 varnish (experimental varnish), Duraphat® (fluoride positive control), placebo varnish (no fluoride), and no treatment (negative control). The varnishes were applied once over the enamel surface using a microbrush. For the in vivo study, 48 Wistar rats were infected with Streptococcus sobrinus 6715, received a treatment, and were submitted to a cariogenic challenge. After 4 weeks, S. sobrinus, oral soft tissue toxicity, presence, and severity of caries were evaluated. For the in situ study, 12 volunteers took part in this randomized crossover, double-blind study performed in four phases of 14 days each. They used intraoral appliances containing four enamel specimens that received the varnish according treatment group. After 24 h, the varnish was removed and plaque accumulation was allowed. A 20% sucrose solution was dripped over the enamel blocks (10×/day for 5 min each). Total streptococci, S. mutans, Lactobacillus, Candida spp. counts, and presence of white spot lesions were evaluated. Lesion depth was also quantified by micro-CT. RESULTS: For the in vivo study, the fluoride (F-varnishes) showed a statistically significant reduction in the percentage of S. sobrinus compared to the negative control (p < 0.05). Toxicological analysis revealed no abnormalities in oral tissues of rats from all groups, and both F-varnishes reduced the number and severity of caries lesions, without significant differences (p < 0.05). No statistical differences in microbiological counts were seen for the in situ experiment (p > 0.05). However, the specimens treated with TiF4 exhibited lower percentage of white spot lesions and the lesion depth was significantly reduced by F-varnishes (p < 0.05). CONCLUSIONS: F-varnishes showed reduction in the percentage of S. sobrinus in vivo, no oral soft tissue toxicity, and a caries-preventive effect in vivo and in situ. CLINICAL RELEVANCE: NaF varnish is largely used due its capacity to form CaF2-like layer on enamel. Therefore, development of studies focused on other fluoride compounds such as a TiF4 varnish, which may have greater efficacy than NaF against tooth demineralization, is important.


Assuntos
Cariostáticos/farmacologia , Cárie Dentária/prevenção & controle , Fluoretos/farmacologia , Titânio/farmacologia , Animais , Estudos Cross-Over , Método Duplo-Cego , Feminino , Fluoretos Tópicos/farmacologia , Humanos , Ratos , Ratos Wistar , Fluoreto de Sódio/farmacologia
19.
Surg Radiol Anat ; 41(4): 469-476, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30552486

RESUMO

The aim of this study was to investigate the association between condylar bone morphological characteristics with occlusal conditions. Besides the study will compare the tomography images with the real condition in 122 temporomandibular joints from 61 skulls. The occlusal conditions were evaluated by number of teeth missing, measurement of overjet and overbite, in millimeters, and presence or absence of crossbite, openbite and dental rotation. The condylar bone morphological conditions were classified in five types (normal, presence of erosion, presence of osteophytes, flattening and/or deformation). This classification was used in real skulls and in Cone Beam Computed tomography (CBCT) images. The data were submitted to statistical analysis with a level of significance of 0.05. Occlusal variables have no association to morphologic data (p > 0.05). Normal condylar bone was seen in 62 CBCT versus 53 in real skulls while morphological alterations were seen in 60 CBCT versus 67-real condyles. The clinical and tomographic measurements were compared, demonstrating an important difference in the classification demonstrating poor association between detection methods (k - 0.3, p < 0.001). The occlusal conditions appear to have no correlation with the morphological condyle conditions. The CBCT is a reliable diagnostic method, although it may present divergences of findings when compared with clinical raw examination to morphologic condylar conditions.


Assuntos
Tomografia Computadorizada de Feixe Cônico , Oclusão Dentária , Artropatias/diagnóstico por imagem , Côndilo Mandibular/diagnóstico por imagem , Articulação Temporomandibular/diagnóstico por imagem , Adulto , Feminino , Humanos , Técnicas In Vitro , Masculino , Crânio/diagnóstico por imagem
20.
Rev. Cient. CRO-RJ (Online) ; 4(3): 37-42, 2019.
Artigo em Inglês | LILACS, BBO - Odontologia | ID: biblio-1052246

RESUMO

Objective: To determine if oral health indicators (dental caries experience andperiodontitis) associate with mental and cardiovascular health issues in AsianAmericans. Methods: Medical history data, dental caries experience (DMFT andDMFS; Decayed, Missing due to caries, Filled Teeth/Surface), and periodontitisstatus of Asian Americans were obtained from the Dental Registry and DNARepository at University of Pittsburgh School of Dental Medicine. A total of 6,117individuals were evaluated and among which dental status of 309 Asian Americansubjects (292 adults and 17 children under the age of twelve) were analyzed. Thefrequency of mental health issues and hypertension depending on dental cariesexperience (Decayed, Missing due to caries, Filled Teeth or Surfaces, DMFT/DMFS)and periodontitis were evaluated and chi-square or Fisher's exact test were usedwith an alpha of 0.05 to determine statistical differences. Results: For the 292Asian American adult subjects, the mean DMFT was 10.1 and mean DMFS was 27.5,which is similar to the expected values in adult DMFT (9.0-11.3) for the UnitedStates. For the 17 Asian American children subjects under age of twelve, the meanDMFT was 1.8 and mean DMFS was 3.8, which also fell into the expected values forchildren DMFT for the United States (1.2-2.6). More severe dental caries experiencewas associated with having an underlying mental health issue (p=0.02) andhypertension (p=0.02). No associations between having periodontitis and mentalor cardiovascular issues were found in the cohort. Conclusion: More severe dentalcaries experience of Asian Americans associate with mental and cardiovascularissues.


Objetivo: Determinar se medidas de saúde oral (cárie dentária e periodontite)estão associadas com doenças cardiovasculares e mentais em asiáticosamericanos. Métodos: Dados de saúde, experiência de cárie (CPOD e CPOS) eperiodontitie de asiáticos americanos foram obtidos do Registro Odontológico eRepositório de DNA da Universidade de Pittsburgh. O total de 6.117 pessoas estavadisponível no registro e 309 asiáticos americanos foram estudados (292 adultos e17 crianças abaixo de 12 anos). As frequências de doença mental e hipertensão,dependendo da experiência de cárie e periodontite, foram avaliadas e os testesqui-quadrado e exato de Fisher foram usados com alfa de 0,05 para determinardiferenças estatísticas. Resultados: A média de CPOD dos 292 adultos foi de 10,1e a média de CPOS foi 27,5, o que é parecido com os valores esperados de CPODde adultos nos Estados Unidos (9,0-11,3). A média de CPOD das 17 crianças menoresde 12 anos foi 1,8 e a do CPOS foi 3,8, o que também é similar aos valores de CPODem crianças dos Estaods Unidos (1,2-2,6). Experiência de cárie mais severaassociou-se com ter problema de saúde mental (p=0,02) e hipertensão (p=0,02).Não se encontrou associação entre periodontite e problemas mentais oucardiovasculares na amostra. Conclusão: Experiência de cárie mais severa emasiáticos americanos associa-se com doença mental e cardiovascular.


Assuntos
Saúde Bucal , Periodontite , Doenças Cardiovasculares , Americanos Asiáticos , Registros Odontológicos , Índice CPO , Cárie Dentária , Transtornos Mentais
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