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1.
J Mech Behav Biomed Mater ; 104: 103637, 2020 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-32174395

RESUMO

OBJECTIVES: To compare the effects of replacing reinforcing barium glass particles by DCPD (dicalcium phosphate dihydrate), as opposed to simply reducing glass filler content, on composite flexural properties and degree of conversion (DC). On a second set of experiments, composites with different "DCPD: glass" ratios were exposed to prolonged water immersion to verify if the presence of DCPD particles increased hydrolytic degradation. METHODS: Two series of composites were prepared: 1) composites with total inorganic content of 50 vol% and "DCPD: glass" ratios ranging from zero (glass only) to 1.0 (DCPD only), in 0.25 increments, and 2) composites containing only silanized glass (from zero to 50 vol%). Disk-shaped specimens were fractured under biaxial flexural loading after 24 h in water. Another set of specimens of composites with different "DCPD: glass" ratios was stored in water for 24 h, 30, 60, 90 and 120 days and tested in flexure. DC was determined using FTIR spectroscopy. Data were analyzed using Kruskal-Wallis/Dunn test (flexural properties) or ANOVA/Tukey test (DC, alpha: 0.05). RESULTS: For glass-only composites, reducing inorganic content caused a linear decrease in strength. The presence of DCPD did not affect composite strength up until a "DCPD: glass" ratio of 0.5. On the other hand, materials with 0.75 and 1.0 DCPD showed significantly lower strength than the glass-only composite with 12.5 vol% filler and the unfilled resin, respectively (p < 0.001). Except for the 0.25 DCPD composite, the presence of DCPD did not contribute to increase flexural modulus. After water storage, composites containing DCPD showed higher percent reductions in properties than the control, but only in a few cases the effect was statistically significant (strength: 0.5 DCPD, modulus: 0.25 and 1.0 DCPD). DC was only marginally affected by DCPD fraction. SIGNIFICANCE: For composites with "DCPD: glass" of 0.25 and 0.5, reductions in strength were related to the lower glass content, and not due to the presence of DCPD. Flexural modulus was primarily defined by glass content. Overall, composites containing DCPD particles presented higher reductions in properties after water storage, but it remained within limits reported for commercial materials.

2.
J Biomed Mater Res B Appl Biomater ; 107(3): 708-715, 2019 04.
Artigo em Inglês | MEDLINE | ID: mdl-30091514

RESUMO

This study describes the synthesis of dicalcium phosphate dihydrate (DCPD) particles in the presence of different ethylene glycol dimethacrylates (EGDMA, ethylene glycol/EG units: 1, 2, 3 or 4) at two monomer-to-ammonium phosphate molar ratios (1:1 and 2:1), as a strategy to develop CaP-monomer particles with improved interaction with resin matrices. Particles displaying high surface areas and organic contents were added to a photocurable BisGMA-TEGDMA resin and the resulting materials were tested for degree of conversion (DC), biaxial flexural strength (BFS), flexural modulus, and ion release. Data were subjected to one-way ANOVA or Kruskal-Wallis/Dunn test (alpha: 0.05). Functionalization with EGDMA derivatives was dependent upon the length of the spacer group and monomer concentration in the synthesis. No differences in DC were observed among materials (p > 0.05). A 39% increase in BFS was obtained with the use of particles with the highest functionalization level compared to non-functionalized particles (p < 0.001). The use of functionalized DCPD reduced flexural modulus in comparison to non-functionalized particles (p < 0.001). Calcium release was similar among materials and remained constant during the experiment, while phosphate release was higher at 7 days in comparison to the remaining weeks (p < 0.001). In conclusion, diethylene glycol dimethacrylate resulted in the highest functionalization levels and the highest BFS among DCPD-containing materials. Ion release was not affected by functionalization. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2018. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B: 708-715, 2019.

3.
Vet Sci ; 5(3)2018 Sep 12.
Artigo em Inglês | MEDLINE | ID: mdl-30213060

RESUMO

Opuntia ficus-indica (L.) Mill. is a xerophylous plant that originated in tropical and subtropical America. This plant is popularly known in Brazil as "palma forrageira" (cactus pear) and plays a fundamental role in animal nutrition, mainly in the Northeastern semi-arid region of the country. The plant has several uses since it presents bioactive compounds that confer biological and pharmacological properties. In this context, the cactus pear can also be considered a potential product to combat parasite infections. The objective of this study was to chemically characterize the O. ficus-indica hydroalcoholic extract (OFIEOH) and to determine its efficacy against gastrointestinal parasites using in vitro tests. Initially, the hydroalcoholic extract from cladode peels of O. ficus-indica was produced by maceration for 21 days. For the chemical characterization, colorimetric dosages were performed for carbohydrates, proteins, phenols and condensed tannins. Liquid chromatography coupled to mass spectrometry/electron spray ionization (LC-MS/ESI) was used to characterize the polyphenolic profile of the OFIEOH extract. Fifteen compounds were identified in the OFIEOH extract, such as methyl, glycosylated and aglycone quercetin derivatives and aglycone and glycosylated kaempferol derivatives. Tri-glycosylated methyl quercetin derivatives were the main compounds identified. In vitro egg hatch (EHT) and larval migration tests (LMT) were used in a range of concentrations of OFIEOH from 12.5 to 100 mg/mL for EHT and 12.5 to 200 mg/mL for LMT. In addition, the LMT was used to test ivermectin (IVM) (from 11.4 to 57.1 µM), associated with the inhibitory concentration of 50% (IC50) for OFIEOH. The combination of OFIEOH (12.5 to 200 mg/mL) plus the IC50 of IVM was also tested. The efficacy of OFIEOH alone varied from 19.33 to 90.0% using the EHT. The LMT revealed an efficacy of 5.78 to 77.26% for the extract. Both tests showed a concentration-dependence inhibitory effect. We found a drug-extract antagonistic neutralizing effect when doses of IVM were added to OFIEOH (maximum efficacy of 73.78%), while a positive additive effect was observed when OFIEOH was added to the IC50 of IVM (IC50 of 82.79 for OFIEOH alone against an IC50 of 55.08 of OFIEOH + IVM). The data from this work indicate that OFIEOH alone may be considered as a suitable ecofriendly product to control gastrointestinal parasites of sheep, offering a more holistic approach to improve animal farming and welfare. The drug-extract interaction is also a promising therapeutic alternative, reducing the final dose to the host, with an optimum combination effect.

4.
Hig. aliment ; 32(280/281): 85-91, 30/06/2018. ilus, tab
Artigo em Português | LILACS | ID: biblio-910584

RESUMO

Este estudo teve por objetivo analisar o perfil calórico e higienicossanitário do leite humano pasteurizado pelo Banco de Leite Humano da Maternidade Barbara Heliodora, localizado no município de Rio Branco-AC. Trata-se de uma pesquisa descritiva e transversal realizada a partir de dados secundários do registro dos exames de teor calórico avaliados pelo crematócrito, e titulação de acidez pelo método de Dornic do leite humano coletado e processado pelo banco de leite humano referente ao período de janeiro a dezembro de 2014. Foram analisadas 499 amostras de leite humano, totalizando 146,39 litros de leite coletados. Deste leite humano pasteurizado 15% foi classificado como hipocalórico, 52% normocalórico e 33% hipercalórico. De acordo com o estágio de lactação, o leite maduro foi o leite com maior quantidade de doações. Em relação à acidez Dornic, encontraram-se resultados dentro da média de titulação aceitável, sendo os maiores valores encontrados na faixa de 4,1 a 7,9% (n=252). As perdas de leite materno representaram 18,34 litros, sendo as principais causas o prazo de validade expirado, quantidade insuficiente para o processamento, sujidade, frasco quebrado e doadora sem cadastro. As doações de leite humano são essenciais para a garantia do leite destinado às crianças que dele necessitam. Assim, o presente estudo concluiu que o leite é normocalórico e está próprio para o consumo, com relação ao alto teor calórico e baixa titulação acidez. É importante o registro dessas informações para que se intensifiquem os estudos nessa área e medidas que busquem melhorar as boas práticas de manipulação.


This study aimed to analyze the caloric and hygienic conditions of the pasteurized breast milk in a Breast Milk Bank in the maternity hospital Barbara Heliodora, in Rio Branco, Ac. This is a descriptive cross-sectional analyzes of secondary data survey of calorie tests by crematocrit, and titratable acidity of the Dornic method of human milk collected and processed by the Breast Milk Bank from January to December 2014. It was analyzed 499 samples of pasteurized breast milk or 146,39 liters. Of those, 15% was hypocaloric, 52% normalcaloric and 33% hypercaloric. The mature milk was the type of milk in more quantity of donations. The Dornic acidity found was between 4,1 to 7,9% (n=252), considered acceptable. A total 18,34 liters of breast milk wasted. The breast milk donations are essential guaranty of milk, designated to the ones in need. Therefore, the present study conclude that the milk is normocaloric and is suitable for consumption. It's important to record those informations so that more studies are performed in this area, besides measures and proceedings of good manipulation practices.


Assuntos
Controle de Qualidade , Aleitamento Materno , Técnicas Microbiológicas , Bancos de Leite , Boas Práticas de Manipulação , Sistema Único de Saúde , Fenômenos Químicos , Saúde Pública
5.
Zebrafish ; 13(3): 177-87, 2016 06.
Artigo em Inglês | MEDLINE | ID: mdl-26909767

RESUMO

The small-molecular compound miglustat (N-butyldeoxynojirimycin, Zavesca(®)) has been approved for clinical use in type 1 Gaucher disease and Niemann-Pick type C disease, which are disorders caused by dysfunction of the endosomal-autophagic-lysosomal system. Miglustat inhibits a number of enzymes involved in glycoconjugate and glycan metabolism, including ß-glucosidase 2 (GBA2), which is exceptionally sensitive to inhibition by miglustat. GBA2 is a glucosylceramide-degrading enzyme that is located on the plasma membrane/endoplasmic reticulum, and is distinct from the lysosomal enzyme glucocerebrosidase (GBA). Various strands of evidence suggest that inhibition of GBA2 contributes to the therapeutic benefits of miglustat. To further explore the pharmacology and biology of GBA2, we investigated whether the zebrafish homolog of GBA2 has similar enzymatic properties and pharmacological sensitivities to its human counterpart. We established that zebrafish has endogenous ß-glucosidase activity toward lipid- and water-soluble GBA2 substrates, which can be inhibited by miglustat, N-butyldeoxygalactonojirimycin, and conduritol B epoxide. ß-Glucosidase activities with highly similar characteristics were expressed in cells transfected with the zebrafish gba2 cDNA and in cells transfected with the human GBA2 cDNA. These results provide a foundation for the use of zebrafish in screening GBA2-targeting molecules, and for wider studies investigating GBA2 biology.


Assuntos
1-Desoxinojirimicina/análogos & derivados , Proteínas de Peixe-Zebra/metabolismo , beta-Glucosidase/metabolismo , 1-Desoxinojirimicina/farmacologia , Sequência de Aminoácidos , Animais , Clonagem Molecular , Regulação Enzimológica da Expressão Gênica , Inibidores de Glicosídeo Hidrolases/farmacologia , Peixe-Zebra , Proteínas de Peixe-Zebra/genética , beta-Glucosidase/genética
6.
Biochem Cell Biol ; 93(6): 581-6, 2015 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-26393781

RESUMO

Glycosphingolipids (GSLs) are hybrid molecules consisting of the sphingolipid ceramide linked to a mono- or oligo-saccharide. In comparison to other membrane lipids, the family of GSLs stands out because of the extensive variation in the carbohydrate headgroup. GSLs are cell surface binding partners, in cis with growth factor receptors, and in trans with bacterial toxins and viruses, and are among the host-derived membrane components of viral particles, including those of HIV. In spite of their biological relevance, GSL profiles of commonly used cell lines have been analyzed to different degrees. Here, we directly compare the GSL complements from CHO-K1, COS-7, HeLa, HEK-293, HEPG2, Jurkat, and SH-SY5Y cells using an HPLC-based method requiring modest amounts of material. Compared to previous studies, the HPLC-based analyses provided more detailed information on the complexity of the cellular GSL complement, qualitatively as well as quantitatively. In particular for cells expressing multiple GSLs, we found higher numbers of GSL species, and different levels of abundance. Our study thus extends our knowledge of biologically relevant lipids in widely used cell lines.


Assuntos
Membrana Celular/metabolismo , Glicoesfingolipídeos/metabolismo , Animais , Linhagem Celular , Linhagem Celular Tumoral , Membrana Celular/química , Células Cultivadas , Chlorocebus aethiops , Cromatografia Líquida de Alta Pressão , Cricetulus , Corantes Fluorescentes/química , Glicosídeo Hidrolases/metabolismo , Glicoesfingolipídeos/química , Hirudo medicinalis/enzimologia , Humanos , Hidrólise , Metabolômica/métodos , Camundongos , Microtecnologia/métodos , Estrutura Molecular , Ratos , Espectrometria de Fluorescência , ortoaminobenzoatos/química
7.
J Proteome Res ; 13(12): 6001-12, 2014 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-25384094

RESUMO

Protein precipitation in organic solvent is an effective strategy to deplete sodium dodecyl sulfate (SDS) ahead of MS analysis. Here we evaluate the recovery of membrane and water-soluble proteins through precipitation with chloroform/methanol/water or with acetone (80%). With each solvent system, membrane protein recovery was greater than 90%, which was generally higher than that of cytosolic proteins. With few exceptions, residual supernatant proteins detected by MS were also detected in the precipitation pellet, having higher MS signal intensity in the pellet fraction. Following precipitation, we present a novel strategy for the quantitative resolubilization of proteins in an MS-compatible solvent system. The pellet is incubated at -20 °C in 80% formic acid/water and then diluted 10-fold with water. Membrane protein recovery matches that of sonication of the pellet in 1% SDS. The resolubilized proteins are stable at room temperature, with no observed formylation as is typical of proteins suspended in formic acid at room temperature. The protocol is applied to the molecular weight determination of membrane proteins from a GELFrEE-fractionated sample of Escherichia coli proteins.


Assuntos
Temperatura Baixa , Formiatos/química , Espectrometria de Massas/métodos , Proteínas de Membrana/análise , Acetona/química , Membrana Celular/metabolismo , Clorofórmio/química , Cromatografia Líquida/métodos , Citosol/metabolismo , Eletroforese em Gel de Poliacrilamida , Proteínas de Escherichia coli/análise , Proteínas de Escherichia coli/química , Precipitação Fracionada/métodos , Proteínas de Membrana/química , Proteínas de Membrana/metabolismo , Metanol/química , Peso Molecular , Proteoma/análise , Proteoma/química , Proteoma/metabolismo , Proteômica/métodos , Reprodutibilidade dos Testes , Solubilidade , Água/química
8.
Br J Sports Med ; 47(18): 1155-60, 2013 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24047570

RESUMO

BACKGROUND: Studies failing to show a negative effect of rapid weight loss (RWL) on performance have been conducted in athletes who have been cycling weight for years. It has been suggested that chronic weight cycling could lead combat athletes to become resistant to the stresses associated with weight loss. To investigate the effects of RWL up to 5% of body mass on high-intensity intermittent performance in weight cyclers (WC) and non-weight cyclers (non-WC). METHODS: Eighteen male combat athletes (WC: n=10; non-WC: n=8) reduced up to 5% of their body mass in 5 days. Body composition, high-intensity performance and plasma lactate were assessed preweight loss and postweight loss. Athletes had 4 h to re-feed and rehydrate following the weigh-in. Food intake was recorded during the weight loss and the recovery periods. RESULTS: Athletes significantly decreased body mass, lean body mass (most likely due to fluid loss) and fat mass following weight loss. No significant changes in performance were found from preweight loss to postweight loss in both groups. Plasma lactate was significantly elevated after exercise in both groups, but no differences were found between groups and in response to RWL. For all these variables no differences were observed between groups. Athletes from both groups ingested high amounts of energy and carbohydrates during the recovery period after the weigh-in. CONCLUSIONS: Chronic weight cycling does not protect athletes from the negative impact of RWL on performance. The time to recover after weigh-in and the patterns of food and fluid ingestion during this period is likely to play the major role in restoring performance to baseline levels.


Assuntos
Adaptação Fisiológica/fisiologia , Desempenho Atlético/fisiologia , Artes Marciais/fisiologia , Perda de Peso/fisiologia , Luta Romana/fisiologia , Adulto , Composição Corporal/fisiologia , Índice de Massa Corporal , Ingestão de Energia/fisiologia , Exercício Físico/fisiologia , Tolerância ao Exercício/fisiologia , Humanos , Ácido Láctico/sangue , Masculino , Adulto Jovem
9.
Rapid Commun Mass Spectrom ; 26(5): 523-31, 2012 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-22302492

RESUMO

RATIONALE: Fluorinated surfactants are being explored as mass spectrometry (MS)-friendly alternatives to sodium dodecyl sulfate (SDS) for proteome analysis. Previous work demonstrates perfluorooctanoic acid (PFOA) to be compatible with electrospray ionization (ESI)-MS. The high volatility of PFOA provides an intrinsic approach to potentially eliminate the surfactant during ESI, or alternatively through solvent evaporation prior to MS. The ammonium salt of PFOA, ammonium perfluorooctanoate (APFO), is likely favored for proteome experiments; the MS and liquid chromatography (LC)/MS tolerance of APFO has not been established for proteome applications. METHODS: Standard proteins and peptides, as well as a yeast proteome mixture, were individually spiked with surfactants (APFO, PFOA, SDS), and subjected to direct infusion ESI-MS, LC/MS/MS and LC/UV. The level of fluorinated surfactant remaining after solvent evaporation under varying conditions (time, pH, salt and protein content) was quantified and compared to the threshold tolerance level of the surfactant in an MS experiment (determined herein). RESULTS: Whereas PFOA is found ineffective at assisting protein solubilization, APFO is as effective as SDS for resolubilization of acetone-precipitated yeast proteins (~100% recovery). Unfortunately, the LC and MS threshold tolerance of APFO is only minimally greater than SDS (~2-fold higher concentration to cause 50% suppression). Nonetheless, the benefits of APFO in a proteome experiment are realized following a one-step evaporation protocol for removal of the surfactant in acidified solvent. CONCLUSIONS: APFO is considered a favoured alternative to SDS for proteome solubilization. Strictly speaking, APFO is not an 'MS-friendly' surfactant for proteome characterization; the detergent not only suppresses ESI signals at high concentration, but also perturbs reversed phase separation. However, the simplicity of APFO removal ahead of LC/MS justifies its use over the conventional SDS.


Assuntos
Caprilatos/química , Fluorcarbonetos/química , Proteoma/análise , Proteômica/métodos , Tensoativos/química , Espectrometria de Massas em Tandem/métodos , Compostos Orgânicos Voláteis/química , Peptídeos/análise , Proteínas/análise , Saccharomyces cerevisiae/química , Proteínas de Saccharomyces cerevisiae/análise , Solubilidade , Espectrometria de Massas por Ionização por Electrospray/métodos
10.
Arq Neuropsiquiatr ; 68(3): 367-71, 2010 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-20602037

RESUMO

People with epilepsy suffer from a considerable lack of physical activity. In addition, an important problem of epilepsy management is the lack of qualified professionals. In this study we present data from a survey which aimed to assess physical educators' general knowledge about epilepsy. One hundred and thirty four physical educators of both sexes answered a questionnaire. Sixty percent of the professionals believe that a seizure is an abnormal electrical discharge of the brain, 13% that epilepsy is a cerebral chronic disease that can not be cured or controlled, 84% that people having convulsions will not necessarily present epilepsy and 5% that people with epilepsy have difficulties of learning. Questions concerned previous professional experience with epilepsy showed that 61% have seen a seizure and 53% have access to some information about epilepsy. Thus, 28% of professionals have a friend or relative with epilepsy, 14% have a student with epilepsy, and 29% helped someone during seizures. Our findings reveal a lack of physical educators' appropriate knowledge about epilepsy. Improvement of this might contribute to the improvement of epilepsy care/management.


Assuntos
Epilepsia , Docentes/estatística & dados numéricos , Conhecimentos, Atitudes e Prática em Saúde , Educação Física e Treinamento , Feminino , Humanos , Masculino , Inquéritos e Questionários
11.
Arq. neuropsiquiatr ; 68(3): 367-371, June 2010. tab
Artigo em Inglês | LILACS | ID: lil-550267

RESUMO

People with epilepsy suffer from a considerable lack of physical activity. In addition, an important problem of epilepsy management is the lack of qualified professionals. In this study we present data from a survey which aimed to assess physical educators' general knowledge about epilepsy. One hundred and thirty four physical educators of both sexes answered a questionnaire. Sixty percent of the professionals believe that a seizure is an abnormal electrical discharge of the brain, 13 percent that epilepsy is a cerebral chronic disease that can not be cured or controlled, 84 percent that people having convulsions will not necessarily present epilepsy and 5 percent that people with epilepsy have difficulties of learning. Questions concerned previous professional experience with epilepsy showed that 61 percent have seen a seizure and 53 percent have access to some information about epilepsy. Thus, 28 percent of professionals have a friend or relative with epilepsy, 14 percent have a student with epilepsy, and 29 percent helped someone during seizures. Our findings reveal a lack of physical educators' appropriate knowledge about epilepsy. Improvement of this might contribute to the improvement of epilepsy care/management.


Pessoas com epilepsia apresentam baixa participação em atividades físicas. Um importante problema nos cuidados da epilepsia é a falta de profissionais qualificados. Neste estudo apresentamos dados de uma pesquisa para avaliar o conhecimento de professores de educação física sobre a epilepsia. Um questionário foi respondido por 134 educadores físicos de ambos os sexos. Sessenta por cento dos profissionais acreditam que a crise epilética é uma descarga elétrica anormal do cérebro, 13 por cento que a epilepsia é uma doença crônica cerebral que não pode ser curada ou controlada, 84 por cento que pessoas que têm convulsões não necessariamente apresentam epilepsia e 5 por cento que pessoas com epilepsia têm dificuldade de aprendizado. Em relação à experiência prévia do profissional, 61 por cento presenciaram uma crise epilética e 53 por cento tiveram acesso a alguma informação sobre epilepsia. Além disso, 28 por cento dos profissionais possuíam amigo ou parente com epilepsia, 14 por cento tinham um aluno com epilepsia e 29 por cento já tinham socorrido alguém durante uma crise. Nossos achados revelam uma falta de conhecimento apropriado dos profissionais da área de educação física sobre a epilepsia. A melhora desse conhecimento pode contribuir para um adequado tratamento e cuidado da pessoa com epilepsia.


Assuntos
Feminino , Humanos , Masculino , Epilepsia , Docentes/estatística & dados numéricos , Conhecimentos, Atitudes e Prática em Saúde , Educação Física e Treinamento , Inquéritos e Questionários
12.
J Proteome Res ; 9(6): 2863-70, 2010 Jun 04.
Artigo em Inglês | MEDLINE | ID: mdl-20377267

RESUMO

SDS has recognized benefits for protein sample preparation, including solubilization and imparting molecular weight separation (e.g., SDS-PAGE). Here, we compare two proteome workflows which incorporate SDS for protein separation, namely, SDS-PAGE coupled to LC/MS (GeLC MS), along with a solution separation platform, GELFrEE, for intact proteome prefractionation and identification. Despite the clear importance of SDS in these and other proteome analysis workflows, the affect of SDS on an LC/MS proteome experiment has not been quantified. We first examined the influence of SDS on both a bottom-up as well as a top-down (intact protein) MS workflow. Surprisingly, at levels up to 0.01% SDS in the injected sample, reliable MS characterization is obtained. We subsequently explored organic precipitation protocols (chloroform/methanol/water and acetone) as a means of lowering SDS, and present a simple modified acetone precipitation protocol which consistently enables MS proteome characterizations from samples initially containing 2% SDS. With this effective strategy for SDS reduction, the GELFrEE MS workflow for bottom-up proteome analysis was characterized relative to GeLC MS. Remarkable agreement in the number and type of identified proteins was obtained from these two separation platforms, validating the use of SDS in solution-phase proteome analysis.


Assuntos
Eletroforese em Gel de Poliacrilamida/métodos , Espectrometria de Massas/métodos , Proteínas/isolamento & purificação , Proteômica/métodos , Dodecilsulfato de Sódio/química , Precipitação Química , Cromatografia Líquida , Peso Molecular , Proteínas/análise , Proteínas/química , Proteoma/análise , Proteoma/química , Reprodutibilidade dos Testes
14.
J. epilepsy clin. neurophysiol ; 13(3): 131-136, Sept. 2007. tab
Artigo em Português | LILACS | ID: lil-471130

RESUMO

INTRODUÇÃO: Pessoas com epilepsia tem sido constantemente desaconselhadas a participarem de atividades físicas ou esportivas por receio do exercício provocar crises epilépticas. Apesar da atitude médica atual recomendando a participação em atividades esportivas, o estigma ainda persiste e as pessoas com epilepsia continuam menos ativas que a população em geral. OBJETIVOS: Neste sentido, vários estudos clínicos e experimentais têm demonstrado um efeito benéfico do exercício físico na epilepsia. O judô é um esporte tradicional e popular e pessoas com epilepsia freqüentemente perguntam aos médicos se podem participar deste tipo de esporte. CONCLUSÃO: Baseado nestas questões, este artigo de revisão propõe avaliar os riscos e benefícios da atividade física em pessoas com epilepsia e discutir o papel do judô neste contexto.


INTRODUCTION: Persons with epilepsy have previously been discouraged from participation in physical activity and sports for fear of inducing seizures or increasing seizure frequency. Despite a shift in medical recommendations toward encouraging rather than restricting participation, the stigma remains and persons with epilepsy continue to be less active than the general population. OBJECTIVES: In these lines, several clinical and experimental studies have demonstrated a positive effect of physical exercise on epilepsy. Judo is a traditional and popular sport and people with epilepsy often ask physicians whether they may engage in this sport. CONCLUSIONS: Based on this, our review article is designed to show the risks and benefits of physical activity in patients with epilepsy and discusses the role of judo in this context.


Assuntos
Humanos , Convulsões/cirurgia , Artes Marciais , Epilepsia/terapia , Atividade Motora
15.
Säo Paulo; Santos; 2003. 73 p. ilus, tab. (BR).
Monografia em Português | LILACS, BBO - Odontologia | ID: lil-345281
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