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1.
J Laparoendosc Adv Surg Tech A ; 30(3): 267-272, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-32053025

RESUMO

Background: Despite the effectiveness of laparoscopic common bile duct (CBD) surgery, no case series details the use and advantages of laparoscopic CBD exploration (LCBDE) without use of intraoperative cholangiography (IOC) in endoscopic retrograde cholangiopancreatography (ERCP) failure. Therefore, we present a case series regarding our success with LCBDE in managing CBD stones (CBDSs) using laparoscopic technique without IOC. Materials and Methods: We performed a descriptive retrospective observational study. Patients with CBDSs, alone or along with gallbladder stones, were treated through LCBDE with primary CBD closure after failed ERCP. Results: All patients underwent LCBDE with choledocotomy and primary duct closure. Patients with gallbladder stones underwent laparoscopic cholecystectomy (78%). All procedures were successful, and no conversions occurred. Surgery duration averaged 106 minutes. Intraoperative bleeding averaged 15 cc, and no mortalities occurred. No patients required additional surgery or intensive care unit admission. Hospitalization duration averaged 5 days. Conclusions: Therefore, a laparoscopic approach with primary CBD closure after failed ERCP for complex CBDSs is safe and effective.

3.
Acta Chir Belg ; : 1-6, 2019 Jul 16.
Artigo em Inglês | MEDLINE | ID: mdl-31307280

RESUMO

Background: The intra-operative classification of appendicitis defines postoperative treatment. The correct designation can influence patient recovery, complications and hospital costs. Recent research has shown that intra-operative classification criteria varies among surgeons, and is not always the same as the pathologist's report. Classification accuracy can lower costs by preventing unnecessary treatment or sub-optimal interventions. Methods: During a period of 4 months, N = 133 appendix specimens were received and evaluated by the pathology department of a single teaching hospital. Five surgeons extracted the specimens and one experienced pathologist drew the histopathology reports. A comparison between the surgeons' classifications and the pathologist's was made. Classification accuracy was determined and statistical analyses was performed using chi-square, and p values were obtained. A p < 0.05 was considered significant. Results: A total of N = 133 specimens were obtained, 127 belonged to patients following emergency surgery due to acute abdominal pain; the other six were from elective hemi-colectomies for right colonic adenocarcinomas, and were not included. Of the 127 specimens analyzed, 14 (11%) were negative, 21 (16.5%) were edematous, 81 (63.7%) were phlegmonous and 11 (8.6%) were gangrenous. A total of 18 (14%) perforated appendices were also reported. Surgical accuracy was 60.6% (N = 67) with a statistically significant p < 0.001. Only five patients with incorrect intraoperative classifications received unnecessary or lacked treatment. Conclusions: An overall accuracy of 60.6% is seen when the surgical classification is compared to the pathological classification. Although the surgeons' accuracy is low when comparing intra-operative versus histopathological classification, this variation in designation does not affect postoperative treatment significantly.

5.
Rev. colomb. cir ; 34(1): 69-74, 20190000. fig
Artigo em Espanhol | LILACS | ID: biblio-982077

RESUMO

Introducción. El hallazgo de divertículos en el intestino delgado es poco usual, y se presentan principalmente en el yeyuno y en el íleon. Tienen una incidencia relativamente baja, aproximadamente, de 0,06 a 5 % en autopsias y de 0,5 a 2,3 % como hallazgos radiológicos. Predominan en la sexta y la séptima década de la vida y en el sexo masculino. Presentación de caso. Se trata de una paciente de sexo femenino de 90 años, que ingresó al servicio de urgencias por un cuadro clínico de un día de dolor en el hemiabdomen derecho irradiado al hipogastrio y asociado con múltiples episodios de emesis. Se hospitalizó para estudios paraclínicos y, durante la observación, presentó deterioro clínico, por lo que fue sometida a una laparotomía exploratoria, y se encontró un divertículo único de yeyuno. Discusión. Los divertículos de intestino delgado son poco frecuentes, y comprometen solo la mucosa y submucosa. Del total de casos, del 0,9 a 1 % corresponden a divertículos del yeyuno. Estos pueden asociarse con divertículos en otra parte del tubo digestivo, como esófago (2,3 %), duodeno (30 %) o colon (61 %). El manejo de esta enfermedad depende de cada paciente


Small intestine diverticulum is a rare finding; it presents more often in the jejunum and ileum. The incidence in autopsies is low (0.06-5%), and 0.5-2.3% as a radiology finding. It is most often found in the 7th decade and in men. A 90-year-old female patient presented with a clinical picture of one day consisting of acute right abdominal pain that radiates to the hypogastrium accompanied by multiple episodes of vomiting. The patient is admitted and a battery of tests are ordered; during observation the patient quickly deteriorates and is taken to an emergency laparotomy, finding a single jejunal diverticulum. Small intestine diverticuli are rare surgical findings (0.9-1%) that involve only the mucosa and submucosa. The majority of small intestinal diverticuli are found in the jejunum. These can be associated with diverticuli in other areas of the GI tract: stomach (2.3%), duodenum (30%) and colon (61%). The management approach depends on each patient's clinical presentation


Assuntos
Humanos , Jejuno , Divertículo , Abdome Agudo , Obstrução Intestinal
6.
Laryngoscope ; 129(5): 1164-1168, 2019 05.
Artigo em Inglês | MEDLINE | ID: mdl-30284284

RESUMO

OBJECTIVE: To compare voice outcomes of autogenous fat injection versus medialization laryngoplasty in patients with glottic insufficiency due to vocal fold paresis or atrophy. METHODS: A retrospective review was performed from 2009 to 2017 of all patients who underwent lipoinjection or medialization laryngoplasty (ML) for glottic insufficiency. Charts were reviewed for demographic information, preoperative diagnosis, surgical intervention, Voice Handicap Index-10 (VHI-10) and Glottal Function Index (GFI) scores, follow-up time (minimum 3 months), and concomitant voice therapy. RESULTS: Eighty-three patients were initially identified and limited to 28 based on the inclusion/exclusion criteria. Thirty-five procedures were performed with a total of 15 fat injections and 20 MLs. The mean age was 60.7 years in the lipoinjection group and 55.6 years in the ML group. There was no significant difference in pretreatment VHI-10 scores between the two groups. Using the lowest recorded voice scores during the follow-up period, both groups had a significant decrease (P < 0.05) compared to preoperative scores (VHI-10: fat decreased from 27.8 to 14.2, ML decreased from 30.5 to 9.1; GFI: fat decreased from 13.7 to 5.27, ML decreased from 13.6 to 4.6). When evaluating the entire follow-up period (median 19 months in fat group, 16.3 months in ML), only the ML group maintained a significant improvement in VHI-10 (median delta 14.5) and GFI (median delta 7) compared to preoperative scores. CONCLUSION: Although both autogenous fat injection and ML result in improved voice scores in the short term, the effect of fat injection appears to be limited, as evidenced by worsening VHI-10 and GFI scores over time. LEVEL OF EVIDENCE: 4 Laryngoscope, 129:1164-1168, 2019.


Assuntos
Glote/patologia , Laringoplastia/métodos , Paralisia das Pregas Vocais/terapia , Voz , Tecido Adiposo , Atrofia/terapia , Feminino , Humanos , Injeções Intralesionais , Masculino , Pessoa de Meia-Idade , Estudos Retrospectivos , Resultado do Tratamento
7.
Int Forum Allergy Rhinol ; 9(1): 39-45, 2019 01.
Artigo em Inglês | MEDLINE | ID: mdl-30216705

RESUMO

BACKGROUND: Inconsistencies in the nomenclature of structures of the frontal sinus have impeded the development of a validated "reference standard" classification system that surgeons can reliably agree upon. The International Frontal Sinus Anatomy Classification (IFAC) system was developed as a consensus document, based on expert opinion, attempting to address this issue. The purposes of this study are to: establish the reliability of the IFAC as a tool for classifying cells in the frontal recess among an international group of rhinologists; and improve communication and teaching of frontal endoscopic sinus surgery (ESS). METHODS: Forty-two computed tomography (CT) scans, each with a marked frontal cell, were reviewed by 15 international fellowship-trained rhinologists. Each marked cell was classified into 1 of 7 categories described in the IFAC, on 2 occasions separated by 2 weeks. Inter- and intrarater reliability were evaluated using Light's kappa (κ), the interclass correlation coefficient (ICC), and simple proportion of agreement. RESULTS: Interrater reliability showed pairwise κ values ranging from 0.7248 to 1.0, with a mean of 0.9162 (SD, 0.0537). The ICC was 0.98. Intrarater reliability showed κ values ranging from 0.8613 to 1.0, with a mean of 0.9407 (SD, 0.0376). The within-rater ICC was 0.98. CONCLUSION: Among a diverse sample of rhinologists (raters), there was substantial to almost perfect agreement between raters, and among individual raters at different timepoints. The IFAC is a reliable tool for classification of cells in the frontal sinus. Further outcome studies are still needed to determine the validity of the IFAC.


Assuntos
Endoscopia/normas , Seio Frontal/anatomia & histologia , Terminologia como Assunto , Consenso , Prova Pericial , Seio Frontal/diagnóstico por imagem , Humanos , Cooperação Internacional , Variações Dependentes do Observador , Reprodutibilidade dos Testes , Tomografia Computadorizada por Raios X
8.
Rev. argent. cardiol ; 84(3): 1-10, jun. 2016. ilus
Artigo em Espanhol | LILACS-Express | ID: biblio-957726

RESUMO

Introducción: El shock cardiogénico es una complicación grave del infarto agudo de miocardio y constituye una de sus principales causas de muerte, pese a lo cual la información en nuestro medio es limitada. Objetivo: Conocer las características clínicas, estrategias de tratamiento y evolución intrahospitalaria del shock cardiogénico en la Argentina. Material y métodos: Se realizó un registro prospectivo, multicéntrico de pacientes internados con shock cardiogénico en el contexto de los síndromes coronarios agudos con y sin elevación del segmento ST entre los años 2013 y 2015 en 64 centros de la Argentina. Resultados: Se incluyeron 165 pacientes, con una edad media de 66 (58-76,5) años; el 65% eran hombres. El 75% de los casos cursaban un síndrome coronario agudo con elevación del segmento ST. El 8,5% estuvieron asociados con complicaciones mecánicas y el 6,7% con compromiso del ventrículo derecho. El 56% presentaban shock cardiogénico al ingreso. Requirieron inotrópicos el 95%, asistencia respiratoria mecánica el 78%, catéter de Swan-Ganz el 44%, balón de contrapulsación intraaór-tico el 37%. El 84% de los síndromes coronarios agudos con elevación del segmento ST (104/124 pacientes) se reperfundieron. La mediana de tiempo desde el inicio de los síntomas al ingreso fue de 240 minutos (132-720). El 80% recibieron angioplastia primaria. La mortalidad intrahospitalaria global fue del 54%, sin diferencias entre los síndromes coronarios agudos con o sin elevación del segmento ST. Asimismo, no hubo diferencia en la frecuencia de eventos y uso de procedimientos entre los síndromes coronarios agudos con o sin elevación del segmento ST. Conclusiones: Las características del shock cardiogénico en la Argentina no difieren mucho de poblaciones de otras partes del mundo. La morbimortalidad es elevada a pesar de la utilización de las estrategias de tratamiento disponibles.

9.
Rev. colomb. cir ; 31(4): 283-288, 20160000. tab, fig
Artigo em Espanhol | LILACS | ID: biblio-884608

RESUMO

Introducción. La hernia de Amyand es una hernia inguinal que contiene el apéndice cecal normal o en un estado de apendicitis aguda. Fue descrita por primera vez en 1735 por Claudius Amyand. La apendicitis aguda probablemente se debe a una obstrucción extraluminal generada por aumento de la presión en el cuello herniario que limita el flujo sanguíneo, en lugar del aumento de la presión intraluminal que se presenta en la apendicitis no asociada con hernias inguinales. Esto resulta en inflamación y proliferación bacteriana, y el apéndice cecal se torna más vulnerable al trauma. Métodos y resultados. Se presenta y analiza, clínica y quirúrgicamente, un caso de hernia de Amyand manejado en el servicio de cirugía general de una institución de tercer nivel de Bogotá; además, se hace una revisión de la literatura científica. El abordaje quirúrgico difirió del propuesto en la guía de manejo de Losanoff y Basson y, sin embargo, se obtuvieron resultados exitosos. Discusión. La hernia de Amyand es una entidad quirúrgica rara, la cual representa un reto diagnóstico y terapéutico, ya que se presenta con características que varían según dos procesos patológicos simultáneos: la hernia inguinal y la apendicitis aguda, cuando esta está presente. El abordaje quirúrgico depende de los hallazgos intraoperatorios, como perforación del apéndice cecal, fístula o pelviperitonitis. Por lo tanto, decidir cuál abordaje quirúrgico es el más apropiado depende de los hallazgos y las preferencias del cirujano. Conclusiones. El tratamiento con apendicectomía, reparo del defecto herniario con malla o ambos, ha suscitado discusión entre los cirujanos por muchos años. Las diferencias de opiniones sobre la técnica quirúrgica más apropiada se deben, entre otras cosas, a la incidencia baja de la hernia de Amyand y a las preferencias quirúrgicas de cada cirujano; el mejor abordaje quirúrgico todavía está en discusión. Sin embargo, la apendicectomía está contraindicada en ciertos pacientes cuando no existe inflamación y, cuando está presente, el uso de la malla todavía es controversial. Por lo tanto, cada reporte de hernia de Amyand y su tratamiento es importante, pues ofrece conocimiento sobre la técnica quirúrgica más apropiada para esta entidad.


Introduction: Amyand´s hernia is an inguinal hernia that contains a normal or an inflamed vermiform appendix. Claudius Amyand first described it in 1735. When acute appendicitis is present, it is believed to be due to extra-luminal obstruction generated by the inguinal ring pressure limiting blood flow, in contrast to the increased intra-luminal pressure that occurs in the acute appendicitis not associated with hernias. This process results in inflammation, bacterial overgrowth, and the appendix becomes more vulnerable to trauma. Methods and Results: We describe and analyze the surgical management of a clinical case of an Amyand´s hernia, managed by the Department of General Surgery of a third level of care institution in Bogotá, Colombia; a literature review was carried out. Despite the availability of the surgical guideline proposed by Losanoff and Basson for the management of the Amyand´s hernia, our surgical approach deviated from this guideline, with both surgical and postoperative success. Discussion: Amyand´s hernia is a rare surgical finding, representing both a diagnostic and a surgical challenge, for it presents with characteristics that vary due to the presence of two simultaneous pathologies: the inguinal hernia and the acute appendicitis if present. The surgical approach depends on the intraoperative findings, such as perforation, fistulas or peritonitis. Therefore, deciding on the best surgical approach depends on the surgical findings and the surgeon's preference. Conclusions: The management of the Amyand´s hernia with appendectomy and/or primary hernia repair with mesh, has been topic of discussion among surgeons for years. This difference in opinion on which surgical approach is the most appropriate, in part is due to the low incidence and rarity of this entity and the surgeon's preference; thus, the best surgical technique is still matter of debate. However, appendectomy is contraindicated in certain patients when there is no inflammation, and when it is present, the use of mesh is still controversial. For this reason each report of Amyand´s hernia and its treatment becomes important, for it provides knowledge in order to choose the most appropriate surgical technique for this rare entity


Assuntos
Humanos , Hérnia Inguinal , Apendicectomia , Apendicite , Apêndice
10.
Rev. argent. cardiol ; 82(5): 373-380, oct. 2014. graf, tab
Artigo em Espanhol | LILACS-Express | ID: lil-734526

RESUMO

Introducción: Las mediciones de calidad ayudan a cuantificar la distancia entre la atención en salud que se brinda y la que se debería brindar. Existen mediciones específicas sobre la calidad de la atención del infarto de miocardio que permiten uniformar los datos de calidad que toda institución debería medir para autoevaluarse y compararse con otras. Objetivo: Analizar los datos de calidad de la atención del infarto en nuestro país utilizando los datos del Registro Multicéntrico SCAR (Síndromes Coronarios Agudos en Argentina). Material y métodos: Se analizaron los datos de calidad de atención del infarto de miocardio de los pacientes de la base de datos del Registro Multicéntrico SCAR utilizando definiciones del documento "ACC/AHA 2008 performance measures for adults with ST-elevation and non-ST-elevation myocardial infarction". Resultados: Se analizaron 751 casos de infarto de miocardio con datos completos sobre indicadores de calidad. El uso de aspirina, betabloqueantes, estatinas y antagonistas de la angiotensina fue cercano al 90%. La excepción fue el uso de clopidogrel, que fue del 72,5% en quienes no recibieron reperfusión mecánica. Se relevó la función ventricular durante la internación en el 90,2% de los casos. Recibieron alguna estrategia de reperfusión el 90,1% de los infartos con elevación del segmento ST y menos de 12 horas de evolución. El tiempo puerta-balón fue < 90 minutos en el 50,8% de los casos, mientras que el tiempo puerta-aguja fue < 30 minutos en el 40,5%. Conclusiones: Globalmente se observaron valores altos de cumplimiento en los tratamientos farmacológicos y de reperfusión, excepto en el uso de clopidogrel sin revascularización mecánica. Se observó un cumplimiento bajo en los tiempos apropiados de los tratamientos de reperfusión.


Introduction: Quality assessments help to quantify the gap between healthcare provision and what should be awarded. There are specific measurements on quality of medical care for myocardial infarction which standardize the quality information that every institution should determine for self-assessment and for comparison with others. Objective: The aim of this study was to analyze quality of care for myocardial infarction data in our country using the SCAR (Acute Coronary Syndromes in Argentina) Multicenter Registry. Methods: Quality of care data for myocardial infarction was analyzed in patients included in the database of the SCAR Multicenter Registry using definitions of the "ACC/AHA 2008 performance measures for adults with ST-elevation and non-ST-elevation myocardial infarction" document. Results: The study analyzed 751 myocardial infarction cases with complete data on quality indicators. Aspirin, betablockers, statins and angiotensin antagonists were used in nearly 90% of patients. The exception was clopidogrel which was used in 72.5% of patients not receiving mechanical reperfusion. Ventricular function was assessed during hospitalization in 90.2% of cases. A reperfusion strategy was used in 90.1% of ST-segment-elevation infarctions and less than 12-hour evolution. Door-to-balloon time was < 90 minutes in 50.8% of cases, while door-to-needle time was < 40.5%. Conclusions: Overall, there was high compliance to pharmacological and reperfusion treatments except in the use of clopidogrel without mechanical revascularization, and low compliance to the appropriate times of reperfusion therapy.

11.
Rev. argent. cardiol ; 82(5): 373-380, oct. 2014. graf, tab
Artigo em Espanhol | BINACIS | ID: bin-131316

RESUMO

Introducción: Las mediciones de calidad ayudan a cuantificar la distancia entre la atención en salud que se brinda y la que se debería brindar. Existen mediciones específicas sobre la calidad de la atención del infarto de miocardio que permiten uniformar los datos de calidad que toda institución debería medir para autoevaluarse y compararse con otras. Objetivo: Analizar los datos de calidad de la atención del infarto en nuestro país utilizando los datos del Registro Multicéntrico SCAR (Síndromes Coronarios Agudos en Argentina). Material y métodos: Se analizaron los datos de calidad de atención del infarto de miocardio de los pacientes de la base de datos del Registro Multicéntrico SCAR utilizando definiciones del documento "ACC/AHA 2008 performance measures for adults with ST-elevation and non-ST-elevation myocardial infarction". Resultados: Se analizaron 751 casos de infarto de miocardio con datos completos sobre indicadores de calidad. El uso de aspirina, betabloqueantes, estatinas y antagonistas de la angiotensina fue cercano al 90%. La excepción fue el uso de clopidogrel, que fue del 72,5% en quienes no recibieron reperfusión mecánica. Se relevó la función ventricular durante la internación en el 90,2% de los casos. Recibieron alguna estrategia de reperfusión el 90,1% de los infartos con elevación del segmento ST y menos de 12 horas de evolución. El tiempo puerta-balón fue < 90 minutos en el 50,8% de los casos, mientras que el tiempo puerta-aguja fue < 30 minutos en el 40,5%. Conclusiones: Globalmente se observaron valores altos de cumplimiento en los tratamientos farmacológicos y de reperfusión, excepto en el uso de clopidogrel sin revascularización mecánica. Se observó un cumplimiento bajo en los tiempos apropiados de los tratamientos de reperfusión.(AU)


Introduction: Quality assessments help to quantify the gap between healthcare provision and what should be awarded. There are specific measurements on quality of medical care for myocardial infarction which standardize the quality information that every institution should determine for self-assessment and for comparison with others. Objective: The aim of this study was to analyze quality of care for myocardial infarction data in our country using the SCAR (Acute Coronary Syndromes in Argentina) Multicenter Registry. Methods: Quality of care data for myocardial infarction was analyzed in patients included in the database of the SCAR Multicenter Registry using definitions of the "ACC/AHA 2008 performance measures for adults with ST-elevation and non-ST-elevation myocardial infarction" document. Results: The study analyzed 751 myocardial infarction cases with complete data on quality indicators. Aspirin, betablockers, statins and angiotensin antagonists were used in nearly 90% of patients. The exception was clopidogrel which was used in 72.5% of patients not receiving mechanical reperfusion. Ventricular function was assessed during hospitalization in 90.2% of cases. A reperfusion strategy was used in 90.1% of ST-segment-elevation infarctions and less than 12-hour evolution. Door-to-balloon time was < 90 minutes in 50.8% of cases, while door-to-needle time was < 40.5%. Conclusions: Overall, there was high compliance to pharmacological and reperfusion treatments except in the use of clopidogrel without mechanical revascularization, and low compliance to the appropriate times of reperfusion therapy.(AU)

12.
Rev. colomb. gastroenterol ; 29(2): 157-160, abr.-jun. 2014. ilus, tab
Artigo em Espanhol | LILACS | ID: lil-722522

RESUMO

El melanoma maligno primario del esófago (MMPE) es una enfermedad extremadamente rara, con una incidencia del 0,1%-0,2% de todos los tumores del esófago. El pronóstico generalmente es pobre, debido a su alto potencial metastásico. El diagnóstico diferencial con el melanoma maligno metastásico puede convertirse en un reto para el grupo tratante y su abordaje requiere de un equipo multidisciplinario, que incluye la participación de un gastroenterólogo, patólogo oncólogo, oncólogo clínico, dermatólogo, entre otros. Se reporta un caso de melanoma maligno primario del esófago in situ en una mujer de 45 años de edad, manejada con esofagectomía en 3 tiempos, en el Instituto Nacional de Cancerología de Colombia; y se realiza una revisión de la literatura.


Primary malignant melanoma of the esophagus is an extremely rare disease: among esophageal tumors its incidence is only 0.1% to 0.2%. The prognosis is generally poor due to the high potential for metastasis. Differential diagnosis of metastatic malignant melanoma can be a challenge for the group treating it. Moreover, managing it requires a multidisciplinary team which includes a gastroenterologist, a pathologist, an oncologist, a medical oncologist, and a dermatologist. We report an in situ case of primary malignant melanoma of the esophagus in a 45 -year-old patient which was managed with three esophagectomies at the National Cancer Institute of Colombia. In addition, we review the literature.


Assuntos
Humanos , Feminino , Pessoa de Meia-Idade , Neoplasias Esofágicas , Esofagoscopia , Cirurgia Geral , Melanoma
13.
Cell Metab ; 19(6): 967-80, 2014 Jun 03.
Artigo em Inglês | MEDLINE | ID: mdl-24746805

RESUMO

Identification of key regulators of lipid metabolism and thermogenic functions has important therapeutic implications for the current obesity and diabetes epidemic. Here, we show that Grb10, a direct substrate of mechanistic/mammalian target of rapamycin (mTOR), is expressed highly in brown adipose tissue, and its expression in white adipose tissue is markedly induced by cold exposure. In adipocytes, mTOR-mediated phosphorylation at Ser501/503 switches the binding preference of Grb10 from the insulin receptor to raptor, leading to the dissociation of raptor from mTOR and downregulation of mTOR complex 1 (mTORC1) signaling. Fat-specific disruption of Grb10 increased mTORC1 signaling in adipose tissues, suppressed lipolysis, and reduced thermogenic function. The effects of Grb10 deficiency on lipolysis and thermogenesis were diminished by rapamycin administration in vivo. Our study has uncovered a unique feedback mechanism regulating mTORC1 signaling in adipose tissues and identified Grb10 as a key regulator of adiposity, thermogenesis, and energy expenditure.


Assuntos
Metabolismo Energético/fisiologia , Proteína Adaptadora GRB10/metabolismo , Lipólise/fisiologia , Complexos Multiproteicos/metabolismo , Serina-Treonina Quinases TOR/metabolismo , Termogênese/fisiologia , Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Tecido Adiposo Marrom/metabolismo , Tecido Adiposo Branco/metabolismo , Animais , Antibióticos Antineoplásicos/farmacologia , Células Cultivadas , Temperatura Baixa , Resposta ao Choque Frio , Diabetes Mellitus , Retroalimentação Fisiológica , Proteína Adaptadora GRB10/biossíntese , Resistência à Insulina , Alvo Mecanístico do Complexo 1 de Rapamicina , Camundongos , Camundongos Knockout , Complexos Multiproteicos/antagonistas & inibidores , Obesidade , Fosfatidilinositol 3-Quinases , Fosforilação , Ligação Proteica , Proteínas Proto-Oncogênicas c-akt , Receptor de Insulina/metabolismo , Proteína Regulatória Associada a mTOR , Transdução de Sinais/fisiologia , Sirolimo/farmacologia , Serina-Treonina Quinases TOR/antagonistas & inibidores
14.
Univ. med ; 53(3): 297-308, jul.-sept. 2012. ilus
Artigo em Espanhol | LILACS | ID: lil-682060

RESUMO

El íleo biliar es una patología poco común y de difícil diagnóstico prequirúgico, pues en la mayoría de los casos se presenta como obstrucción intestinal sin síntomas biliares asociados. En el artículo se presentan dos casos del 2010, cada uno con características diferentes respecto a la localización de la fístula y la evolución postoperatoria: una fístula colecistogástrica con obstrucción del íleon terminal y una fístula colecistoduodenal con obstrucción del yeyuno medio. En el primero se hizo una resolución del íleo biliar y en el mismo tiempo quirúrgico la colecistectomía y cierre de la fístula; mientras que en el otro se trató únicamente el cuadro obstructivo. Dado que en la literatura no existe una amplia revisión de esta situación clínica, no hay consenso en cuanto a la resolución de la patología biliar en el mismo tiempo quirúrgico. Por la evolución clínica de los pacientes tan diversa, se evaluó el problema...


Gallstone ileus is an uncommon pathology, difficultto diagnose on a pre-surgical way, mostly sometimespresents as intestinal obstruction withoutbiliary symptoms associated. In 2010, it presentedtwo cases, each one with different characteristicsregarding the fistula location and post-operativeevolution: cholecystogastric fistula and obstructionof the terminal ileus and cholecystoduodenalfistula and obstruction of the medial yeyunum.First was resolved with colecystectomy and fistulaclosure. The second was trated only by obstructionresolution. Since in literature there isn’t acomprehensive review of this type of pathology,there is not agreement in the management of thebiliary pathology at the same surgical time, andtaking in account the different evolution of ourtwo patients, is the reason that we were interestedin the evaluation of this problem...


Assuntos
Colecistectomia , Fístula , Obstrução Intestinal , Íleus/diagnóstico , Íleus/patologia
15.
J Biol Chem ; 287(31): 26087-93, 2012 Jul 27.
Artigo em Inglês | MEDLINE | ID: mdl-22685300

RESUMO

APPL1 is an adaptor protein that plays a critical role in regulating adiponectin and insulin signaling. However, how APPL1 is regulated under normal and pathological conditions remains largely unknown. In this study, we show that APPL1 undergoes phosphorylation at Ser(430) and that this phosphorylation is enhanced in the liver of obese mice displaying insulin resistance. In cultured mouse hepatocytes, APPL1 phosphorylation at Ser(430) is stimulated by phorbol 12-myristate 13-acetate, an activator of classic PKC isoforms, and by the endoplasmic reticulum (ER) stress inducer, thapsigargin. Overexpression of wild-type but not dominant negative PKCα increases APPL1 phosphorylation at Ser(430) in mouse hepatocytes. In addition, suppressing PKCα expression by shRNA in hepatocytes reduces ER stress-induced APPL1 phosphorylation at Ser(430) as well as the inhibitory effect of ER stress on insulin-stimulated Akt phosphorylation. Consistent with a negative regulatory role of APPL1 phosphorylation at Ser(430) in insulin signaling, overexpression of APPL1(S430D) but not APPL1(S430A) impairs the potentiating effect of APPL1 on insulin-stimulated Akt phosphorylation at Thr(308). Taken together, our results identify APPL1 as a novel target in ER stress-induced insulin resistance and PKCα as the kinase mediating ER stress-induced phosphorylation of APPL1 at Ser(430).


Assuntos
Proteínas Adaptadoras de Transdução de Sinal/metabolismo , Estresse do Retículo Endoplasmático , Hepatócitos/metabolismo , Resistência à Insulina , Processamento de Proteína Pós-Traducional , Animais , Linhagem Celular , Ativadores de Enzimas/farmacologia , Hepatócitos/enzimologia , Humanos , Insulina/fisiologia , Isoenzimas/metabolismo , Fígado/enzimologia , Fígado/metabolismo , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Camundongos Obesos , Obesidade/metabolismo , Fosforilação , Proteína Quinase C-alfa/metabolismo , Serina/metabolismo , Transdução de Sinais , Acetato de Tetradecanoilforbol/farmacologia
16.
Rev. colomb. cir ; 27(2): 121-128, abr. 2012. tab
Artigo em Espanhol | LILACS-Express | ID: lil-650049

RESUMO

Se presenta un estudio restrospectivo observacional de dos cohortes obtenidas en cuatro años, con un total de 215 pacientes, 92 para apendicectomía laparoscópica y 123 para apendicectomía convencional, comparando variables como estancia hospitalaria, tiempo quirúrgico, complicaciones y dolor posoperatorio, entre otras. Se halló mayor frecuencia de complicaciones en la apendicectomía tradicional y mayor incidencia de infección del sitio operatorio (p<0,05).


Retrospective observational study of two cohorts of patients in a period of time of 4 years, with a total of 215 patients, 92 operated by laparoscopy and 123 open was completef at our hospital.. Length of stay, operating room times, postoperative complications, and other items were compared. The results show a higher rate of complications in open appendectomy with a clearly higher incidence of superficial wound infection (p < 0,05).

17.
Vaccine ; 30(2): 273-9, 2012 Jan 05.
Artigo em Inglês | MEDLINE | ID: mdl-22079077

RESUMO

Red deer (Cervus elaphus) and white-tailed deer (Odocoileus virginianus) are hosts for different tick species and tick-borne pathogens and play a role in tick dispersal and maintenance in some regions. These factors stress the importance of controlling tick infestations in deer and several methods such as culling and acaricide treatment have been used. Tick vaccines are a cost-effective alternative for tick control that reduced cattle tick infestations and tick-borne pathogens prevalence while reducing the use of acaricides. Our hypothesis is that vaccination with vector protective antigens can be used for the control of tick infestations in deer. Herein, three experiments were conducted to characterize (1) the antibody response in red deer immunized with recombinant BM86, the antigen included in commercial tick vaccines, (2) the antibody response and control of cattle tick infestations in white-tailed deer immunized with recombinant BM86 or tick subolesin (SUB) and experimentally infested with Rhipicephalus (Boophilus) microplus, and (3) the antibody response and control of Hyalomma spp. and Rhipicephalus spp. field tick infestations in red deer immunized with mosquito akirin (AKR), the SUB ortholog and candidate protective antigen against different tick species and other ectoparasites. The results showed that deer produced an antibody response that correlated with the reduction in tick infestations and was similar to other hosts vaccinated previously with these antigens. The overall vaccine efficacy was similar between BM86 (E=76%) and SUB (E=83%) for the control of R. microplus infestations in white-tailed deer. The field trial in red deer showed a 25-33% (18-40% when only infested deer were considered) reduction in tick infestations, 14-20 weeks after the first immunization. These results demonstrated that vaccination with vector protective antigens could be used as an alternative method for the control of tick infestations in deer to reduce tick populations and dispersal in regions where deer are relevant hosts for these ectoparasites.


Assuntos
Antígenos/imunologia , Proteínas de Artrópodes/imunologia , Proteínas de Insetos/imunologia , Ixodes/imunologia , Glicoproteínas de Membrana/imunologia , Proteínas Recombinantes/imunologia , Infestações por Carrapato/veterinária , Vacinação/métodos , Vacinas/imunologia , Animais , Antígenos/administração & dosagem , Proteínas de Artrópodes/administração & dosagem , Cervos , Feminino , Proteínas de Insetos/administração & dosagem , Masculino , Glicoproteínas de Membrana/administração & dosagem , Proteínas Recombinantes/administração & dosagem , Infestações por Carrapato/prevenção & controle , Vacinas/administração & dosagem
18.
Cultur Divers Ethnic Minor Psychol ; 17(3): 325-30, 2011 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-21787065

RESUMO

The universality of cognitive processes has been called into question. Research suggests that individuals from Eastern cultures (e.g., China, Korea) when compared to individuals from Western cultures (e.g., the United States) prefer to reason holistically. This line of research has not been extended to cultural groups far removed from cultures traditionally surveyed in cross-cultural research such as Hispanics. We conducted two studies to understand: 1) the generalizability of the construct of holistic reasoning in Mexicans, and 2) the preferred reasoning style of Mexicans when compared to U.S. Americans. Results support the generalization of the factor structure of holistic reasoning as originally hypothesized by Choi, Koo, and Choi (2007). The results of Study 2 suggest that Mexicans scored higher than U.S. Americans on certain aspects of holistic reasoning.


Assuntos
Cognição , Comparação Transcultural , Características Culturais , Americanos Mexicanos/psicologia , Pensamento , Adulto , Análise Fatorial , Feminino , Humanos , Masculino , México , Resolução de Problemas , Testes Psicológicos , Reprodutibilidade dos Testes , Estados Unidos , Adulto Jovem
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