Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 22
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Chem Asian J ; 14(16): 2846-2852, 2019 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-31228325

RESUMO

Three dinuclear dysprosium(III) complexes, [Dy2 L2 (O2 CPh)2 ]⋅2 MeOH (1), [Dy2 L2 {(2-NO2 )O2 CPh}2 ] (2), and [Dy2 L2 {(2-OH)O2 CPh}2 ]⋅MeOH⋅MeCN (3) (H2 L=N1 ,N3 -bis(4-chlorosalicyladehyde)diethylenetriamine), have been synthesized and structurally characterized. Complexes 1-3 possess similar Ln2 cores and differ in substituents at the benzyl rings of benzoates. Direct current (dc) magnetic susceptibility studies in the 2-300 K range showed weak antiferromagnetic interactions between two dysprosium(III) ions in 1-3. The alternating current (ac) magnetic susceptibility measurements indicated that they all exhibited SMM behavior. The strategic attachment of the -NO2 group (in 2) and the -OH functionality (in 3) on the skeleton of the benzoic acid led to subtle variations of the bond lengths and bond angles in the coordination environments of the central dysprosium(III) ions, consequently resulting in the enhancement of the energy barriers for 2 and 3. Complete-active-space self-consistent field (CASSCF) calculations were employed to rationalize the experimental outcomes. Theoretical calculations confirm the existence of antiferromagnetic interactions in 1-3, and the calculated dc magnetic susceptibility data agree well with those obtained experimentally. The computational results reveal more axial g tensors, as well as higher first excited Kramers doublets in 2 and 3; thus resulting in higher energy barriers in compounds 2 and 3.

2.
Dalton Trans ; 48(27): 10337-10345, 2019 Jul 21.
Artigo em Inglês | MEDLINE | ID: mdl-31211300

RESUMO

Eleven new lanthanide complexes based on a bis-tridentate nitronyl nitroxide radical NIT-Pm2Py (2-(4,6-di(pyridin-2-yl)pyrimidin-2-yl)-4,4,5,5-tetramethyl-4,5-dihydro-1H-imidazol-1-oxy-3-oxide), namely (NIT-Pm2Py)Ln(hfac)3 (Ln = Gd (1Gd), Tb (2Tb), Dy (3Dy), Ho (4Ho), Er (5Er), Yb (6Yb)), [(NIT-Pm2Py)Ln2(hfac)6]·xH2O (Ln = Gd (7Gd), Tb (8Tb), Ho (10Ho), x = 0.5 for 7Gd and 1 for 8Tb and 10Ho) and (NIT-Pm2Py)Ln2(hfac)6 (Ln = Dy (9Dy), Er (11Er)) were prepared and characterized. These complexes can be selectively prepared by controlling the reaction ratio of Ln(hfac)3·2H2O to the radical ligand NIT-Pm2Py. Single crystal X-ray crystallographic analyses confirmed that 1Gd-6Yb are isostructural 2p-4f LnIII-radical complexes, in which the NIT-Pm2Py radical acts as a terminal tridentate ligand chelating to one LnIII ion. On the other hand, 7Gd-11Er are isostructural 4f-2p-4f LnIII-radical-LnIII complexes with the NIT-Pm2Py acting as a bridging ligand between two LnIII ions. 7Gd-11Er represent a rare family of complexes showing the NIT bridged 4f-2p-4f three-spin motif. Alternating-current (ac) magnetic susceptibility investigations revealed that complex 6Yb exhibits field-induced frequency dependence, suggesting a possible field-induced single-molecule magnet behavior. Ab initio calculations were performed on all these complexes. The fitting of the magnetic susceptibilities of these complexes indicates weak antiferromagnetic coupling between the LnIII and NIT radical.

3.
Chem Asian J ; 14(15): 2620-2628, 2019 Aug 01.
Artigo em Inglês | MEDLINE | ID: mdl-31066179

RESUMO

The employment of a new rigid N-tridentate ligand, bis(1-chloroimidazo[1,5-a]pyridin-3-yl)pyridine (bcpp), in the construction of cobalt(II) single-ion magnets is reported. Two cobalt(II) complexes, [Co(bcpp)Cl2 ] (1) and [Co(bcpp)Br2 ] (2), have been prepared and characterized. Single-crystal XRD analyses reveal that complexes 1 and 2 are isostructural. They are pentacoordinated mononuclear cobalt(II) compounds with expected trigonal bipyramidal geometry. Both analysis of the magnetic data and ab initio calculations reveal easy-plane magnetic anisotropy (D>0) for 1 and 2. Detailed alternating current magnetic susceptibility measurements reveal the occurrence of slow magnetic relaxation behavior for the cobalt(II) centers of 1 and 2; thus indicating that both complexes are field-induced single-ion magnets.

4.
Chem Asian J ; 14(7): 986-994, 2019 Apr 01.
Artigo em Inglês | MEDLINE | ID: mdl-30628179

RESUMO

To explore the influences of the subtle structural variations in the ligand backbones on the single-molecule magnetic properties of dinuclear dysprosium(III) complexes, three ligands-H2 L1 (H2 L1 =N1 ,N3 -bis(salicylaldehyde)diethylenetriamine), H2 L2 (H2 L2 =N1 ,N3 -bis(3-methoxysalicylidene)diethylenetriamine), and H2 L3 (H2 L3 =N1 ,N3 -bis(5-chlorosalicyladehyde)diethylenetriamine)-were synthesized and employed to prepare the expected dinuclear dysprosium(III) complexes. The three ligands differ in terms of the substituents at the benzene rings of the salicylaldehyde moieties. The reactions of Dy(NO3 )3 ⋅6 H2 O, pivalic acid, and the ligands H2 L1 , H2 L2 , and H2 L3 generated complexes with the formulae [Dy2 (L1 )2 (piv)2 ] (1), [Dy2 (L2 )2 (piv)2 ] (2), and [Dy2 (L3 )2 (piv)2 ]⋅ 2 MeCN (3), respectively. The purposeful attachment of the functional groups with varied sizes at the benzene rings of the salicylaldehyde backbones resulted in slight differences in the Dy-O-Dy bond angles and the Dy⋅⋅⋅Dy bond lengths in 1-3; consequently, the three complexes exhibited distinct magnetic properties. They all showed slow magnetization relaxation with energy barriers of 40.32 (1), 31.67 (2), and 33.53 K (3). Complete active space self-consistent field (CASSCF) calculations were performed on complexes 1-3 to rationalize the slight discrepancy observed in the magnetic behavior. The calculated results satisfactorily explained the experimental outcomes.

6.
Inorg Chem ; 58(2): 1301-1308, 2019 Jan 22.
Artigo em Inglês | MEDLINE | ID: mdl-30592214

RESUMO

A family of dinuclear dysprosium cores bridged by different ligands within a polyoxometalates (POMs) framework, (TBA)8.5H1.5[(PW11O39)2Dy2X2(H2O)2]·6H2O (X = OH (1), F (2), OAc (3); TBA = tetra- n-butylammonium), was successfully synthesized and structurally characterized. Magnetic studies indicate that the bridging ligands can significantly affect the magnetic behaviors, with 1 and 3 showing antiferromagnetic coupling and 2 bridged by fluoride ions showing ferromagnetic interaction. 1 and 2 behaved as single-molecule magnets (SMMs) with the thermally activated energy barrier of 98(5) and 74(6) cm-1 under zero dc filed, respectively, whereas no SMM behavior was observed for 3 bridged by two µ-η1:η2-acetato ligands. Notably, the low-temperature fluorescence spectra of 1-3 provide valuable information on the energy levels, which are consistent with the anisotropic barriers determined by magnetic measurements. These results offer an insight into the magneto-optical correlation. Furthermore, the effective energy barrier of 1 reaches a breakthrough among all POM-based SMMs.

7.
Chem Sci ; 9(41): 7986-7991, 2018 Nov 07.
Artigo em Inglês | MEDLINE | ID: mdl-30450182

RESUMO

The on-off switching of spin-crossover (SCO) and single-molecule magnetism (SMM) remains highly attractive, especially if it involves dynamic crystal-to-crystal transformation. Herein we report the first molecule, a mononuclear cobalt(ii) complex, that exhibits on-off switching between SCO and SMM reversibly during crystal-to-crystal transformation. Subtle structural transformation triggered by a simple dehydration-rehydration process induces significant geometrical changes of the CoII center and modification of the supramolecular interactions and switches its colour and magnetic properties (dark red/SCO-on/SMM-off ↔ orange/SCO-off/SMM-on). This work suggests that modification of the weak supramolecular interactions could be very effective in achieving switchable materials involving both SCO and SMM properties.

8.
Chem Commun (Camb) ; 54(85): 12105-12108, 2018 Oct 23.
Artigo em Inglês | MEDLINE | ID: mdl-30302448

RESUMO

The double chloride-bridged dimer of a dinuclear dysprosium(iii) single-molecule magnet (SMM) was successfully isolated by assembling centrosymmetric dinuclear Dy2 SMMs. Such structural transformation involves the generation and cleavage of chloride bridges and leads to consecutive transformations of one- and two-step slow relaxation of magnetization.

9.
Org Biomol Chem ; 16(44): 8513-8524, 2018 11 14.
Artigo em Inglês | MEDLINE | ID: mdl-30357224

RESUMO

Baefrutones A-F (1-6), six new meroterpenoids with rare triketone-phloroglucinol-monoterpene/sesquiterpene frameworks, together with their biosynthetically related intermediate (±)-baeckenon B (7), were isolated from the aerial part of Baeckea frutescens under the guidance of HPLC-Q/TOF-MS2 investigation. Compounds 1-4 represent the first examples of natural meroterpenoids existing as four pairs of inseparable diastereomeric atropisomers (2 : 1, 1H NMR integration) caused by the restricted rotation around the C-6-C-7-C-1' bonds arising from the intramolecular hydrogen bond between C-1 carbonyl and 2'-OH. The discovery of these architectures not only largely enriched the chemodiversity of the meroterpenoid and atropisomer library, but also might be exciting and challenging for asymmetric organic synthesis. Their structures and absolute configurations were established by extensive spectroscopic analysis, X-ray diffraction, and ECD calculations. Compounds 5 and 6 were biomimetically synthesized from 7 and ß-caryophyllene via a regioselective oxidative hetero-Diels-Alder reaction, thus providing access to the construction of the 6/6/9/4 tetracyclic ring system. The anti-inflammatory activities of these meroterpenoids were also discussed.

10.
Inorg Chem ; 57(3): 1408-1416, 2018 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-29314838

RESUMO

A new type of dinuclear dysprosium(III) complex based on phthalocyanine and salicylaldehyde derivatives (HL-R), [Dy2(Pc)2(L-R)2(H2O)]·2THF (R = OCH3 (1), OC2H5 (2); H2Pc = phthalocyanine; HL-OCH3 = 2-hydroxy-3-methoxybenzaldehyde; HL-OC2H5 = 3-ethoxy-2-hydroxybenzaldehyde), was successfully synthesized and structurally characterized. Complex 1 features a sandwich-type triple-decker structure, where two coplanar L-OCH3 ligands lie in the middle layer shared by two eight-coordinated DyIII ions and two Pc ligands are located in the outer layer. In 2, the introduction of an ethoxy group generates a noncoordination mode for the Oalkoxy atom. Magnetic studies indicate that complex 1 behaves as a zero-field single-molecule magnet with a higher energy barrier, while 2 exhibits a fast tunneling relaxation process. Theoretical calculations revealed that changes in the ligand field environment around DyIII ions can significantly affect the arrangement of the main magnetic axes and further result in distinct magnetic interactions as well as different relaxation behaviors.

11.
Zhongguo Zhong Yao Za Zhi ; 42(20): 3847-3859, 2017 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-29243417

RESUMO

Andrographolide is one of the major natural active constituents in Andrographis panniculata of Acanthaceae family. Andrographolide and its derivatives are proved to posses a wide range of biological activities, such as anti-inflammatory, antiviral, anti-tumor, anti-human immunodeficiency virus(HIV), hepatoprotective and α-glucosidase inhibition activity. In this paper, the development of synthesis of rographolide derivatives and their related pharmacological activities were reviewed.


Assuntos
Andrographis/química , Diterpenos/síntese química , Diterpenos/farmacologia
12.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 24(6): 1698-1704, 2016 Dec.
Artigo em Chinês | MEDLINE | ID: mdl-28024479

RESUMO

OBJECTIVE: To construct eukaryotic expression vector of siRNA specific for BCR/ABL and to investigate the effect of recombinant plasmid on BCR/ABL and P210 protein expression in K562 cells. METHODS: siRNA(small interfering RNA)was designed according to the Tuschl's principle of Ai-based medicine, and was converted into cDNA coding expression of shRNA(small hairpin RNAs)of siRNA for BCR/ABL fusion gene. The cDNA was synthesized and inserted into plasmid pTER. The pTER117 and pTER363 of recombinant plasmid being eukaryotic expression vector was controlled by the H1 promoter of RNA polymerase III, and identified by the restriction map and the sequence analysis. The recombinant plasmid did not only have the screening resisting antibiotics, its expression but also are induced by tetracycline (tet). After steadily transfection into K562 cells by Lipofectamine, their positive mono-cell clones being resistant to Zeocin were isolated. TaqMan real-time quantitative RT-PCR (RQ-PCR) and Western blot respectively detected expression of BCR/ABL mRNA and P210 protein. Trypaum blue dying was used to analyze the proliferation of K562 cells. Cell apoptosis was observed by flow cytometer. RESULTS: the recombinant plasmid was steadily transfected into K562 cells by Lipofectamine 2000, Their positive mono-cell clones being resistant to Zeocin were isolated. The proliferation of K562 cells were remarkably inhibited by the recombinant plasmid induced gene expression by tetracycline. Tetracycline induced its expression for 48 h and 72 h. pTER117, pTER363 decreased the mRNA level of BCR/ABL 90%, 82% and 91.5%, 84%, respectively, P210 protein were almost measured in K562 cells. FCM analysis showed that the recombinant plasmid induced apoptosis in K562 cells, the apoptosis rate were respectively 34.4%, 58.1% in K562 cells treated by pTER117 for 48 h and 72 h, apoptosis rate were 31.8%, 54.6% by pTER363, but the control groups did not show these effects on K562 cells. CONCLUSION: The siRNA eukaryotic expression vector against BCR/ABL mRNA has been successfully conctructed,and effectively inhibits the expression of BCR/ABL in K562 cells, inhibite cell growth and induce cell apoptosis.


Assuntos
Eucariotos , RNA Interferente Pequeno , Apoptose , Ciclo Celular , Proliferação de Células , Citometria de Fluxo , Proteínas de Fusão bcr-abl , Humanos , Células K562 , Plasmídeos , RNA Mensageiro , Transfecção
13.
Sci Total Environ ; 569-570: 548-556, 2016 Nov 01.
Artigo em Inglês | MEDLINE | ID: mdl-27371770

RESUMO

Synchronized online measurements of gas- and particle- phase organics including non-methane hydrocarbons (NMHCs), oxygenated volatile organic compounds (OVOCs) and submicron organic matters (OM) were conducted in November 2010 at Heshan, Guangdong provincial supersite, China. Several biomass burning events were identified by using acetonitrile as a tracer, and enhancement ratios (EnRs) of organics to carbon monoxide (CO) obtained from this work generally agree with those from rice straw burning in previous studies. The influences of biomass burning on NMHCs, OVOCs and OM were explored by comparing biomass burning impacted plumes (BB plumes) and non-biomass burning plumes (non-BB plumes). A photochemical age-based parameterization method was used to characterize primary emission and chemical behavior of those three organic groups. The emission ratios (EmRs) of NMHCs, OVOCs and OM to CO increased by 27-71%, 34-55% and 67% in BB plumes, respectively, in comparison with non-BB plumes. The estimated formation rate of secondary organic aerosol (SOA) in BB plumes was found to be 24% faster than non-BB plumes. By applying the above emission ratios to the whole PRD, the annual emissions of VOCs and OM from open burning of crop residues would be 56.4 and 3.8Gg in 2010 in PRD, respectively.


Assuntos
Aerossóis/análise , Poluentes Atmosféricos/análise , Biomassa , Fogo , Hidrocarbonetos/análise , Compostos Orgânicos Voláteis/análise , China , Monitoramento Ambiental
14.
Ying Yong Sheng Tai Xue Bao ; 27(3): 794-802, 2016 Mar.
Artigo em Chinês | MEDLINE | ID: mdl-29726184

RESUMO

The impacts of three kinds of human disturbances, i.e., non-grazing, reseeding and free-grazing, on plant community characters, i.e., species composition, composition of functional groups, species diversity, and aboveground biomass, were studied in a typical steppe in Inner Mongolia, China. The results showed that different disturbances had significant impacts on the structure and diversity of plant functional groups. The number of plant species, aboveground biomass, community diversity, and richness indices were the highest in the non-grazing treatment, with the fi-gures of 22, 171.32 g·m-2, 1.46 and 5.7, respectively. The importance value (IV), percentage, aboveground biomass, diversity, richness, and evenness indices of shrub and sub-shrub, and perennial grasses were also the highest in the non-grazing treatment. In contrast, the IV, species percentage, aboveground biomass, diversity, and evenness indices of perennial forbs were the lowest in the non-grazing treatment. The aboveground biomass of mesophyte, C3 and C4 plants was the highe-st with the figures of 22.22, 143.35 and 27.97 g·m-2 respectively in the non-grazing treatment. The aboveground biomass of mesoxerophyte was highest (13.60 g·m-2), the species percentage of xerophyte was lowest (48.5%) and that of C4 plants was highest (28.8%) in the reseeding treatment. The species percentage of annual or biennial herbs was highest (12.3%), while that of mesophyte and C4 plants was lowest (17.0% and 20.9%) in the free-grazing treatment. The non-grazing treatment showed a beneficial effect for restoration of degraded grassland system.


Assuntos
Biodiversidade , Pradaria , Plantas/classificação , Biomassa , China , Recuperação e Remediação Ambiental , Poaceae
15.
Int J Clin Exp Med ; 8(1): 1150-5, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25785106

RESUMO

BACKGROUND: Upper lumbar disc herniation (ULDH) is easy to be misdiagnosed due to its special anatomical and atypical clinical features. Few studies have identified the relationship between ULDH and adjacent wedge-shaped vertebrae (WSV). HYPOTHESIS: WSV may have some indicative relations withULDH. PATIENTS AND METHODS: Between January 2003 and October 2013, 47 patients (27 males and 20 females; mean age, 41.2 years) with single-level ULDH (as study group) and 47 sex- and age-matched healthy volunteers (as control group) were studied by radiograph. The two groups were compared with respect to age, sexual proportion, body mass index (BMI), kyphotic angle, and the proportion of WSV. Also, correlative analyses were conducted in the study group to investigate the relation between the kyphotic angle of target vertebrae and other factors including age, BMI, Cobb angle, JOA score and bone mineral density (BMD). RESULTS: The average kyphotic angle in the study group was 11° (4°-22°), while the average kyphotic angle in the control group was 2° (0°-7°). Obviously, the mean kyphotic angle in the study group was statistically larger than that in the control group (t=13.797, P<0.001). The proportion of WSV in the study group was significantly larger than that in the control group (x(2)=36.380, P<0.0001). The correlations between kyphotic angles and other items (i.e., age, BMI, BMD, Cobb angle and JOA score) in the study group and the control group were low or uncorrelated. CONCLUSIONS: WSV are indicatively associated with adjacent ULDH. Thus, ULDH should be alerted when WSV are first found in radiograph and accompanied by clinical symptoms.

16.
Apoptosis ; 20(3): 348-57, 2015 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-25576195

RESUMO

In our previous study, 17ß-estradiol was proved to protect rat annulus fibrosus cells against apoptosis induced by interleukin-1ß (IL-1ß). However, whether 17ß-estradiol has protective effect on rat nucleus pulposus cells remains unclear. The purpose of this study was to further explore the effects of 17ß-estradiol on rat nucleus pulposus cells based on IL-1ß-induced apoptosis. TUNEL assay and Annexin V/PI double staining were used to detect apoptosis and revealed that IL-1ß induced notable apoptosis, which was reversed by 17ß-estradiol. Meanwhile, cell viability and binding ability were decreased by IL-1ß, but activated caspase-3 was increased. However, all of the detected effects of IL-1ß were eliminated by 17ß-estradiol. Furthermore, real-time quantitative RT-PCR was used to further find that IL-1ß downregulated expression level of type II collagen, aggrecan, tissue inhibitor of matrix metalloproteinase (TIMP)-1, while upregulated matrix metalloproteinase (MMP)-3, MMP-13 and Bcl-2, which was further confirmed by western blot. Finally, 17ß-estradiol was proved to abolish the above negative effects of IL-1ß. In summary, this work presented that IL-1ß maybe induced apoptosis of rat nucleus pulposus cells, which was resisted by 17ß-estradiol by down-regulating MMP-3 and MMP-13 via a mitochondrial pathway. This research provides a novel insight into the anti-apoptotic effect of 17ß-estradiol on IL-1ß-induced cytotoxicity, and may potentially lead to a better understanding of the clinical effects of 17ß-estradiol, especially in terms of intervertebral disc degeneration.


Assuntos
Estradiol/farmacologia , Interleucina-1beta/antagonistas & inibidores , Metaloproteinase 13 da Matriz/genética , Metaloproteinase 3 da Matriz/genética , Substâncias Protetoras/farmacologia , Agrecanas/genética , Agrecanas/metabolismo , Animais , Apoptose/efeitos dos fármacos , Caspase 3/genética , Caspase 3/metabolismo , Sobrevivência Celular/efeitos dos fármacos , Condrócitos/citologia , Condrócitos/efeitos dos fármacos , Condrócitos/metabolismo , Colágeno Tipo II/genética , Colágeno Tipo II/metabolismo , Regulação da Expressão Gênica , Interleucina-1beta/farmacologia , Disco Intervertebral/citologia , Disco Intervertebral/efeitos dos fármacos , Disco Intervertebral/metabolismo , Masculino , Metaloproteinase 13 da Matriz/metabolismo , Metaloproteinase 3 da Matriz/metabolismo , Mitocôndrias/efeitos dos fármacos , Mitocôndrias/metabolismo , Cultura Primária de Células , Proteínas Proto-Oncogênicas c-bcl-2/genética , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Ratos , Ratos Sprague-Dawley , Transdução de Sinais , Inibidor Tecidual de Metaloproteinase-1/genética , Inibidor Tecidual de Metaloproteinase-1/metabolismo
17.
World J Gastroenterol ; 20(25): 8139-50, 2014 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-25009386

RESUMO

AIM: To screen lymph nodes metastasis associated long noncoding RNAs (lncRNAs) in colorectal cancer through microarray analysis. METHODS: Metastatic lymph node (MLN), normal lymph node (NLN) and tumor tissues of 3 colorectal cancer (CRC) patients were collected during the operation and validated by pathological examinations. RNAs were extracted from MLN, NLN, and cancer tissues separately. RNA quantity and quality were measured with a NanoDrop ND-1000 spectrophotometer and RNA integrity was assessed by standard denaturing agarose electrophoresis. Agilent Feature Extraction Software (Version 11.0.1.1) was used to analyze acquired array images. Four differently expressed lncRNAs were confirmed by quantitative real-time polymerase chain reaction (qRT-PCR) in 26 subsets of MLN, NLN, and tumor tissues. RESULTS: Of 33045 lncRNAs, 1133 were differentially expressed in MLN compared with NLN, of which 260 were up-regulated and 873 down-regulated (≥ 2 fold-change). Five hundred and forty-five lncRNAs were differentially expressed in MLN compared with tumor tissues, of which 460 were up-regulated and 85 down-regulated (≥ 2 fold-change). Compared with NLN and cancer tissues, 14 lncRNAs were specifically up-regulated and 5 specifically down-regulated in MLN. AK307796, ENST00000425785, and AK021444 were confirmed to be specifically up-regulated in MLN and ENST00000465846 specifically down-regulated in MLN by qRT-PCR in 26 CRC patients. CONCLUSION: The specifically expressed lncRNAs in MLN may exert a partial or key role in the progress of lymph nodes metastasis of CRC.


Assuntos
Biomarcadores Tumorais/genética , Neoplasias Colorretais/genética , Neoplasias Colorretais/secundário , Perfilação da Expressão Gênica/métodos , Testes Genéticos/métodos , Linfonodos/química , Linfonodos/patologia , Análise de Sequência com Séries de Oligonucleotídeos , RNA Longo não Codificante/genética , RNA Neoplásico/genética , Análise por Conglomerados , Regulação Neoplásica da Expressão Gênica , Humanos , Metástase Linfática , Valor Preditivo dos Testes , Reação em Cadeia da Polimerase em Tempo Real , Reação em Cadeia da Polimerase Via Transcriptase Reversa
18.
Artigo em Chinês | MEDLINE | ID: mdl-22919761

RESUMO

OBJECTIVE: To develop a best method of constructing influenza NP fusion gene containing enhanced green fluorescent protein (EGFP). METHODS: The full-length NP gene of influenza A was amplified by RT-PCR and was inserted into an eukaryotic expression vector pEGFP-N1 in order to construct a fusion gene of pEGFP-N1-NP using three different methods. Method one, NP gene containing restriction endonucleases and pEGFP-N1 were both digested using the same restrict enzymes and ligated, yielding the fusion gene of pEGFP-N1-NP. Method two, NP gene was cloned into pMD19-T Vector to construct a plasmid of pMD19-T-NP. The pMD19-T-NP cloned into pEGFP-N1 to construct the fusion gene of pEGFP-N1-NP; Method three, NP gene containing restriction endonucleases was cloned into pMD19-T Simple Vector to construct a plasmid of pMD19-T-NP. The pMD19-T-NP cloned into pEGFP-N1 to construct the fusion gene of pEGFP-N1-NP. RESULTS: The fusion gene of recombinant eukaryotic expression vector pEGFP-N1-NP was successfully constructed by using method three. CONCLUSIONS: The full-length NP gene is obtained and its fusion gene of recombinant eukaryotic expression plasmid is successfully constructed. This study provides foundation for further understanding the biological function of NP protein and the mechanism of diseases induced by influenza A virus.


Assuntos
Fusão Gênica Artificial , Proteínas de Fluorescência Verde/genética , Proteínas de Ligação a RNA/genética , Proteínas do Core Viral/genética , Clonagem Molecular , Vetores Genéticos , Reação em Cadeia da Polimerase
19.
Cancer Invest ; 29(3): 208-13, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-21314329

RESUMO

Biological therapies can be beneficial in cancer patients. The present study aims to examine the inhibitory mechanism of curcumin on cancer cells in patients with colorectal cancer. The results showed that curcumin administration increased body weight, decreased serum TNF-alpha levels, increased apoptotic tumor cells, enhanced expression of p53 molecule in tumor tissue, and modulated tumor cell apoptotic pathway. We conclude that the curcumin treatment improves the general health of patients with colorectal cancer via the mechanism of increased p53 molecule expression in tumor cells and consequently speeds up tumor cell apoptosis.


Assuntos
Antineoplásicos/uso terapêutico , Peso Corporal/efeitos dos fármacos , Caquexia/tratamento farmacológico , Neoplasias Colorretais/tratamento farmacológico , Curcumina/uso terapêutico , Proteína Supressora de Tumor p53/metabolismo , Apoptose/efeitos dos fármacos , Caquexia/etiologia , Neoplasias Colorretais/complicações , Neoplasias Colorretais/metabolismo , Terapia Combinada , Procedimentos Cirúrgicos do Sistema Digestório , Método Duplo-Cego , Ensaio de Imunoadsorção Enzimática , Feminino , Humanos , Imuno-Histoquímica , Marcação In Situ das Extremidades Cortadas , Masculino , Estadiamento de Neoplasias , Radioterapia , Fator de Necrose Tumoral alfa/sangue , Proteína Supressora de Tumor p53/efeitos dos fármacos , Regulação para Cima
20.
Artigo em Chinês | MEDLINE | ID: mdl-19544661

RESUMO

OBJECTIVE: Through observing the morphology and topography of the prepared influenza viruses (H1N1) treated with the different Nonidet P-40 solutions using atomic force microscopy (AFM), to explore the application of AFM on the research of the internal character of viral morphology and structural virology. METHODS: The virus samples were treated with serial diluted Nonidet P-40 solutions from 0.05% to 0.20% and then investigated by AFM with the tapping mode in air at room temperature to obtain the morphology and topography changes including height data,amplitude data and phase data for both spherical and filamentous influenza virus A. RESULTS: The serial AFM images show that the erosion degree of the virions is proportional with the improvement of NP-40 concentration,and partly denuded virion image appeared at 0.05% NP-40 treatment, which was revealed clearly on both amplitude images and phase images. CONCLUSION: This work demonstrated for the first time that the internal topography of influenza virion could be revealed by AFM via suitable nonionic surfactants chemical dissection.


Assuntos
Vírus da Influenza A/química , Microscopia de Força Atômica/instrumentação , Orthomyxoviridae/química , Polietilenoglicóis/farmacologia , Humanos , Vírus da Influenza A/efeitos dos fármacos , Nanoestruturas , Orthomyxoviridae/efeitos dos fármacos , Espermicidas/farmacologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA