Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 52
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
Int J Biol Macromol ; 147: 453-462, 2020 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-31923519

RESUMO

Due to the favorable stability, water solubility and good biocompatibility, carbon dots have attracted much attention. Herein, a novel nitrogen-doping bifunctional carbon dots (N-BCDs) with ultra-highly quantum yield (QYabs = 70.4%) is prepared through microwave-assisted method. 50 µg/mL of N-BCDs emit intense fluorescence in HeLa and GES-1 cells with negligible cytotoxicity. In addition, effective inhibition of N-BCDs to human insulin (HI) fibrillation is observed even at 10:1 (mass ratio of HI: N-BCDs) by ThT fluorescence, CD assay and TEM. N-BCDs prevent HI from fibrillation with prolonged lag time and reduced fluorescent intensity at equilibrium, regardless of the addition time of N-BCDs (HI: N-BCDs = 1:1, mass ratio), which has been rarely reported before. Furthermore, the morphology of final HI fibrils is shorter and thinner in the presence of N-BCDs. Mechanism studies reveal that the enhanced hydrogen bond between HI monomers and N-BCDs inhibits nucleation during the lag stage (Ka: 1.54 × 104 L·mol-1, 298 K), while the accumulation of N-BCDs blocks the growth of profibrils in the elongation stage. To the best of our knowledge, it's the first time to observe the accumulation of N-BCDs around HI profibrils with TEM. Our study provides a new strategy for developing efficient nanoparticle inhibitors for protein fibrillation.

2.
J Virol ; 94(4)2020 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-31748388

RESUMO

The majority of antibodies induced by influenza neuraminidase (NA), like those against hemagglutinin (HA), are relatively specific to viruses isolated within a limited time window, as seen in serological studies and the analysis of many murine monoclonal antibodies (MAbs). We report three broadly reactive human MAbs targeting N1 NA. Two were isolated from a young adult vaccinated with trivalent influenza vaccine (TIV), which inhibited N1 NA from viruses isolated from humans over a period of a hundred years. The third antibody, isolated from a child with acute mild H7N9 infection, inhibited both group 1 N1 and group 2 N9 NAs. In addition, the antibodies cross-inhibited the N1 NAs of highly pathogenic avian H5N1 influenza viruses. These antibodies are protective in prophylaxis against seasonal H1N1 viruses in mice. This study demonstrates that human antibodies to N1 NA with exceptional cross-reactivity can be recalled by vaccination and highlights the importance of standardizing the NA antigen in seasonal vaccines to offer optimal protection.IMPORTANCE Antibodies to the influenza virus NA can provide protection against influenza disease. Analysis of human antibodies to NA lags behind that of antibodies to HA. We show that human monoclonal antibodies against NA induced by vaccination and infection can be very broadly reactive, with the ability to inhibit a wide spectrum of N1 NAs on viruses isolated between 1918 and 2018. This suggests that antibodies to NA may be a useful therapy and that the efficacy of influenza vaccines could be enhanced by ensuring the appropriate content of NA antigen.

3.
Arch Insect Biochem Physiol ; 103(2): e21634, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31587360

RESUMO

Pteromalus puparum is a gregarious pupal endoparasitoid with a wide host range. It deposits eggs into pierid and papilionid butterfly pupae. Glutathione S-transferases (GSTs) are a family of multifunctional detoxification enzymes that act in xenobiotic metabolism in insects. Insect genome projects have facilitated identification and characterization of GST family members. We identified 20 putative GSTs in the P. puparum genome, including 19 cytosolic and one microsomal. Phylogenetic analysis showed that P. puparum GSTs are clustered into Hymenoptera-specific branches. Transcriptomic data of embryos, larvae, female pupae, male pupae, female adults, male adults, venom glands, carcass, salivary glands, and ovaries revealed stage-, sex-, and tissue-specific expression patterns of GSTs in P. puparum. This is the most comprehensive study of genome-wide identification, characterization, and expression profiling of GST family in hymenopterans. Our results provide valuable information for understanding the metabolic adaptation of this wasp.


Assuntos
Glutationa Transferase/genética , Proteínas de Insetos/genética , Vespas/genética , Sequência de Aminoácidos , Animais , Embrião não Mamífero/química , Embrião não Mamífero/metabolismo , Feminino , Perfilação da Expressão Gênica , Glutationa Transferase/química , Glutationa Transferase/metabolismo , Proteínas de Insetos/química , Proteínas de Insetos/metabolismo , Larva/genética , Larva/metabolismo , Masculino , Filogenia , Pupa/genética , Pupa/metabolismo , Alinhamento de Sequência , Vespas/crescimento & desenvolvimento , Vespas/metabolismo
4.
Front Physiol ; 10: 1282, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31680999

RESUMO

The immunological interaction between Drosophila melanogaster and its larval parasitoids has been thoroughly investigated, however, little is known about the interaction between the host and its pupal parasitoids. Pachycrepoideus vindemmiae, a pupal ectoparasitoid of D. melanogaster, injects venom into its host while laying eggs on the puparium, which regulates host immunity and interrupts host development. To resist the invasion of parasitic wasps, various immune defense strategies have been developed in their hosts as a consequence of co-evolution. In this study, we mainly focused on the host immunomodulation by P. vindemmiae and thoroughly investigated cellular and humoral immune response, including cell adherence, cell viability, hemolymph melanization and the Toll, Imd, and JAK/STAT immune pathways. Our results indicated that venom had a significant inhibitory effect on lamellocyte adherence and induced plasmatocyte cell death. Venom injection and in vitro incubation strongly inhibited hemolymph melanization. More in-depth investigation revealed that the Toll and Imd immune pathways were immediately activated upon parasitization, followed by the JAK/STAT pathway, which was activated within the first 24 h post-parasitism. These regulatory effects were further validated by qPCR. Our present study manifested that P. vindemmiae regulated the cellular and humoral immune system of host D. melanogaster in many aspects. These findings lay the groundwork for studying the immunological interaction between D. melanogaster and its pupal parasitoid.

5.
Artigo em Inglês | MEDLINE | ID: mdl-31414136

RESUMO

BACKGROUND: In preclinical studies, the Intracerebral Microinjection Instrument (IMI) has demonstrated the ability to deliver therapeutics within the brain in 3-dimensional arrays from a single overlying penetration while incurring minimal localized trauma. OBJECTIVE: To evaluate the safety and performance of the IMI in its first use in humans to deliver stem cells in complex configurations within brain regions affected by ischemic injury. METHODS: As part of a phase 1 study, 3 chronically hemiparetic motor stroke patients received intracerebral grafts of the therapeutic stem cell line, NSI-566, using the IMI and its supporting surgical planning software. The patients were 37 to 54 yr old, had ischemic strokes more than 1 yr prior to transplantation, and received Fugl-Meyer motor scale scores of 17-48 at screening. During a single surgical procedure, patients received several neural grafts (42 ± 3) within the peri-infarct region targeted strategically to facilitate neural repair. RESULTS: The IMI enabled multiple cellular deposits to be safely placed peripheral to stroke lesions. The procedure was well tolerated, recovery was uneventful, and there occurred no subsequent complications. The IMI performed reliably throughout the procedures without evident targeting errors. One year after transplantation, all 3 subjects displayed significant clinical improvement, and imaging analysis demonstrated occupation of infarct cavities with new tissue without tumor formation. CONCLUSION: IMI technology permits unprecedented numbers of injections to be tactically placed in 3-dimensional arrays safely and reliably in human subjects.This advanced methodology can optimize the benefits of novel therapeutics by enabling versatile 3-dimensional intracerebral targeting.

6.
World J Gastroenterol ; 25(25): 3242-3255, 2019 Jul 07.
Artigo em Inglês | MEDLINE | ID: mdl-31333315

RESUMO

BACKGROUND: Ulcerative colitis (UC) is considered to be closely associated with alteration of intestinal microorganisms. According to the traditional Chinese medicine (TCM) theory, UC can be divided into two disease syndromes called Pi-Xu-Shi-Yun (PXSY) and Da-Chang-Shi-Re (DCSR). The relationships among gut microbiota, TCM syndromes, and UC pathogenesis have not been well investigated. AIM: To investigate the role of gut microbiota in UC and the distinction of microbiota dysbiosis between PXSY and DCSR syndromes. METHODS: From May 2015 to February 2016, UC patients presenting to LongHua Hospital who met the established inclusion and exclusion criteria were enrolled in this retrospective study. Fresh stool specimens of UC patients with PXSY or DCSR were collected. The feces of the control group came from the health examination population of Longhua Hospital. The composition of gut bacterial communities in stool samples was determined by the pyrosequencing of 16S ribosomal RNA. The high-throughput sequencing reads were processed with QIIME, and biological functions were predicted using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States. RESULTS: The composition of gut bacterial communities in 93 stool samples (30 healthy controls, 32 patients with PXSY syndrome, and 31 patients with DCSR syndrome) was determined by the pyrosequencing of 16S ribosomal RNA. Beta diversity showed that the composition of the microbiota was different among the three groups. At the family level, Porphyromonadaceae, Rikeneliaceae, and Lachnospiraceae significantly decreased while Enterococcus, Streptococcus, and other potential pathogens significantly increased in UC patients compared to healthy subjects. At the genus level, Parabacteroides, Dorea, and Ruminococcus decreased while Faeca-libacterium showed increased abundance in UC compared to healthy controls. Five differential taxa were identified between PXSY and DCSR syndromes. At the genus level, a significantly increased abundance of Streptococcus was observed in DCSR patients, while Lachnoclostridium increased in PXSY patients. The differential functional pathways of the gut microbiome between the PXSY and DCSR groups mainly included lipid metabolism, immunity, and the metabolism of polypeptides. CONCLUSION: Our study suggests that the gut microbiota contributes to the distinction between the two TCM syndromes of UC.


Assuntos
Bactérias/isolamento & purificação , Colite Ulcerativa/diagnóstico , Disbiose/diagnóstico , Microbioma Gastrointestinal , Medicina Tradicional Chinesa/métodos , Adulto , Bactérias/genética , Colite Ulcerativa/microbiologia , Colo/microbiologia , DNA Bacteriano/isolamento & purificação , Disbiose/microbiologia , Feminino , Humanos , Mucosa Intestinal/microbiologia , Masculino , Pessoa de Meia-Idade , RNA Ribossômico 16S/genética , Estudos Retrospectivos , Síndrome
7.
J Colloid Interface Sci ; 551: 101-110, 2019 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-31075625

RESUMO

Carbon dots (C-dots) are superior in the aspects of excellent water solubility, good biocompatibility, environmentally friendliness and non-blinking fluorescence. In this work, highly photoluminescent small-size C-dots (QY = 18.8%, quinine sulfate as standard) with narrow size distribution (1.70 ±â€¯0.21 nm) have been synthesized by using citric acid and triethylamine through hydrothermal method. The optimal excitation and emission wavelength of C-dots are 350 nm and 437 nm, respectively. And the prepared C-dots display excitation-independent behavior due to less surface defects and uniform size. Interestingly, the fluorescence of C-dots could be rapidly and selectively quenched by Hg2+ within 200 s at room temperature without further modification. Under optimal conditions, the limit of detection (LOD) was measured to be nanomolar level (2.8 nM) with a linear range of 0.05-7 µM, lower than the previous published reports. Furthermore, our results reveal that static quenching mechanism was dominated in the process in which Hg2+ coordinate with the oxygen-containing groups of C-dots to form nonfluorescent complexes. And only the addition of Hg2+ destroyed the surface defects of C-dots resulting in the fluorescent quenching. The presence of other common interfering metal ions reported in previous literature (Ag+, Cu2+, Fe3+) do not affect the surface defects, which has rarely been reported before. Besides, this sensing platform has been further successfully applied to the label free detection of Hg2+ in tap water and living cells. These conclusions demonstrate the great potential of our C-dots in selective detection of environmental and cellular Hg2+, which may achieve a lot of achievements in clinical diagnosis and other biological researches.


Assuntos
Carbono/química , Corantes Fluorescentes/química , Mercúrio/análise , Pontos Quânticos/química , Poluentes Químicos da Água/análise , Técnicas Biossensoriais/métodos , Cátions Bivalentes , Linhagem Celular , Sobrevivência Celular/efeitos dos fármacos , Humanos , Limite de Detecção , Nitrogênio/química , Imagem Óptica/métodos , Oxigênio/química , Tamanho da Partícula , Sensibilidade e Especificidade , Espectrometria de Fluorescência , Propriedades de Superfície
8.
Colloids Surf B Biointerfaces ; 177: 219-227, 2019 May 01.
Artigo em Inglês | MEDLINE | ID: mdl-30743069

RESUMO

Intracellular reactive oxygen species (ROS) generation are associated with many diseases. Lots of studies focus on the detection of intracellular ROS by small fluorescent molecules. However, ROS recognized by biocompatible nanoparticles are relatively less reported. It is widely known that albumin-based nanomaterials possess unique advantages in biomedical applications because they are biodegradable and biocompatible. Herein, fluorescent protein nanoparticles (PNPs) were prepared using BSA as a starting material without introducing extra fluorescent molecules. The blue fluorescent PNPs were well characterized by FL, FTIR, CD, TEM, DLS, etc. It was revealed that the PNPs exhibited two types of emissive centers through FL spectra and the fluorescence lifetimes. Further mechanism study indicated that the fluorescence of the PNPs was mainly derived from three kinds of aromatic amino acids, namely tryptophan, tyrosine and phenylalanine. Moreover, the fluorescence properties of the PNPs were tightly related to pH. The PNPs displayed excellent stabilities under harsh conditions as well as physiological conditions. In addition, the PNPs (200 µg/mL) were nontoxic to HeLa and GES-1 cell lines, showing good biocompatibility. The cellular uptake of PNPs was occurred only when the cells were stressed with glucose oxidase or H2O2, thereafter the bright blue fluorescence was observed, indicating that it could be utilized for the recognition of cellular oxidation damage. These findings will offer novel clues for the future synthesis of even brighter protein nanoparticles and their biomedical applications.


Assuntos
Proteínas Luminescentes/química , Nanopartículas/química , Soroalbumina Bovina/química , Animais , Bovinos , Linhagem Celular , Células HeLa , Humanos , Concentração de Íons de Hidrogênio , Proteínas Luminescentes/síntese química , Imagem Óptica , Oxirredução , Tamanho da Partícula , Soroalbumina Bovina/síntese química , Propriedades de Superfície , Raios Ultravioleta
10.
Int J Pharm ; 556: 338-348, 2019 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-30553955

RESUMO

Controlled drug-delivery systems have potential as substitutes for traditional medication systems due to the advantages in safety, efficacy, and patient compliance that these long-acting dosage forms provide. In this context, the present study focus on the development of self-implanted hyaluronic acid (HA) tiny needles that encapsulate ivermectin (IVM)-poly (lactic-co-glycolic acid) (PLGA) microparticles for controlled transdermal IVM release to treat parasitic diseases. The fabricated tiny needles involved matching portable applicator have potentially able for self-administration by patients without intense pain or complexity of current controlled-release devices. The biodegradable IVM-loaded PLGA microparticles were prepared and encapsulated within the tip of dissolving HA tiny needles to achieve high delivery efficiency. The drug loading of tiny needles might be controlled by varying the repeat time of filling or pressing processes. In-vitro tests showed that the tiny needles have sufficient mechanical strength to be inserted into skin within seconds and, next rapidly dissolved to release the loaded drug carriers into subcutaneous tissues for intradermal sustained IVM release. With the in-vivo test in rats, the insertion site recovered barrier property within 3 h. In comparison to traditional hypodermic injection or implantation of controlled-release systems, the proposed polymer tiny needles can be considered as a promising device for controlled transdermal drug delivery.


Assuntos
Antiparasitários/administração & dosagem , Sistemas de Liberação de Medicamentos , Ácido Hialurônico/química , Ivermectina/administração & dosagem , Administração Cutânea , Animais , Antiparasitários/química , Química Farmacêutica/métodos , Preparações de Ação Retardada , Portadores de Fármacos/química , Liberação Controlada de Fármacos , Feminino , Ivermectina/química , Camundongos , Agulhas , Copolímero de Ácido Poliláctico e Ácido Poliglicólico/química , Polímeros/química , Ratos , Ratos Sprague-Dawley , Autoadministração
11.
World J Gastroenterol ; 24(37): 4263-4271, 2018 Oct 07.
Artigo em Inglês | MEDLINE | ID: mdl-30310259

RESUMO

AIM: To identify functional proteins involved in pancreatic-duodenal homeobox-1 (PDX1)-mediated effects on gastric carcinogenesis. METHODS: A PDX1-overexpressed model was established by transfecting gastric cancer cell line SGC7901 with pcDNA3.1(+)-PDX1 vector (SGC-PDX1). Transfection with empty pcDNA3.1 vector (SGC-pcDNA) served as control. Comparative protein profiles of the two groups were analyzed by two-dimensional electrophoresis based-proteomics (2DE gel-based proteomics). The differential proteins identified by 2DE were further validated by qRT-PCR and immunoblotting. Finally, co-immunoprecipitation was used to determine any direct interactions between PDX1 and the differential proteins. RESULTS: 2DE gel proteomics identified seven differential proteins in SGC-PDX1 when compared with those in SGC-pcDNA. These included four heat shock proteins (HSPs; HSP70p1B, HSP70p8, HSP60, HSP27) and three other proteins (ER60, laminin receptor 1, similar to epsilon isoform of 14-3-3 protein). Immunoblotting validated the expression of the HSPs (HSP70, HSP60, HSP27). Furthermore, their expressions were lowered to 80%, 20% and 24%, respectively, in SGC-PDX1, while PDX1 exhibited a 9-fold increase, compared to SGC-pcDNA. However, qRT-PCR analysis revealed that mRNA levels of the HSPs were increased in SGC-PDX1, suggesting that the expression of the HSPs was post-translationally regulated by the PDX1 protein. Finally, co-immunoprecipitation failed to identify any direct interaction between PDX1 and HSP70 proteins. CONCLUSION: This study demonstrates the potential involvement of HSPs in PDX1-mediated effects on the genesis of gastric cancer.


Assuntos
Regulação Neoplásica da Expressão Gênica , Proteínas de Choque Térmico/metabolismo , Proteínas de Homeodomínio/metabolismo , Neoplasias Gástricas/metabolismo , Transativadores/metabolismo , Biomarcadores Tumorais/metabolismo , Linhagem Celular Tumoral , Regulação para Baixo , Eletroforese em Gel Bidimensional , Perfilação da Expressão Gênica , Genes Homeobox , Proteínas de Choque Térmico HSP70/metabolismo , Humanos , Processamento de Imagem Assistida por Computador , Pâncreas/metabolismo , Processamento de Proteína Pós-Traducional , Proteômica , RNA Mensageiro/metabolismo
12.
Zhongguo Shi Yan Xue Ye Xue Za Zhi ; 26(1): 268-272, 2018 Feb.
Artigo em Chinês | MEDLINE | ID: mdl-29397856

RESUMO

OBJECTIVE: To explore the occurrence and various influencing factors of the splenic extramedullary hematopoiesis in rats. METHODS: A total of 411 rats of different sex and species were assigned to this study. These rats were fed different feed in the same environment, and killed after 104 weeks. The spleen of all animals was embedded in paraffin, sectioned, stained with hematoxylin and eosin(H& E), then examined by optical microscopy to observe splenic extramedullary hematopoiesis. RESULTS: At the end of the study, it was found that the splenic extramedullary hematopoiesis occurred in 116 animals (28.22%). The splenic extramedullary hematopoiesis that included the erythroid, granulocytic and megakaryocytic lineages (23.11%); the inciderce of splenic extramedullary hematopoiesis in Wistar rats was higher than that in SD rats; the incidence of splenic extramedullary hematopoiesis in female was higher than that in male; the feed had no effect on splenic extramedullary hematopoiesis of all animals; different species and different feed were also did not involve in the level of splenic extramedullary hematopoiesis. CONCLUSION: Species and sex show effect on the incidence of splenic extramedullary hematopoiesis, but does not involve in the level of splenic extramedullary hematopoiesis; feed had no influences on all indexes of splenic extramedullary hematopoiesis. The results of this study may provide a reference for the study of the splenic extramedullary hematopoiesis of the aging human.


Assuntos
Hematopoese Extramedular , Animais , Feminino , Hematopoese , Masculino , Megacariócitos , Ratos , Ratos Sprague-Dawley , Ratos Wistar , Baço
14.
Nurse Educ Today ; 49: 79-83, 2017 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-27889582

RESUMO

BACKGROUND: Professional attitude is of great importance for nursing talents in the modern society. To develop an effective educational program for student nurses in China, an appropriate instrument is required for the assessment of their professional attitude. OBJECTIVE: To assess the validity and reliability of the Instrument of Professional Attitude for Student Nurses (IPASN) in Chinese version. METHODS: The original version of IPASN was translated through Brislin model (translation, back translation, culture adaption and pilot study) with the authorization from the developer. A total of 681 nursing students were chosen by stratified convenience sampling to assess construct validity using exploratory factor analysis (EFA). Besides, item analysis, Cronbach's alpha coefficients, test-retest reliability were conducted to test the psychometric properties in this part. A total of 204 nursing undergraduate trainees were selected by cluster convenience sampling to confirm the structure using confirmatory factor analysis (CFA) in another time. RESULTS: Corrected item-total correlations, alpha if item deleted were between 0.33 and 0.69, 0.906 and 0.913, respectively, indicating no item should be deleted. Cronbach alpha value was 0.91 for the total scale and Cronbach alpha coefficient for subscales ranged from 0.67 to 0.89. Test-retest reliability estimated from intraclass correlation coefficient (ICC) was 0.74 (P<0.05). Differences in item scores between the high-score group (the first 27%) and low-score group (the last 27%) were significant (P<0.001), indicating that the item discrimination ability was good. Seven subscales (contribution to increase of scientific information load, autonomy, community service, continuous education, to promote professional development, cooperation and theory guiding practice) were identified in EFA and confirmed in CFA, and explained 65.5% of the total variance. CONCLUSION: It indicated that the Chinese version of IPASN was valid and reliable for the evaluation of nursing students' professional attitude.


Assuntos
Profissionalismo/normas , Psicometria/normas , Reprodutibilidade dos Testes , Estudantes de Enfermagem/psicologia , Tradução , Atitude , China , Feminino , Humanos , Masculino , Psicometria/métodos , Adulto Jovem
15.
Biomater Sci ; 5(2): 247-257, 2017 Jan 31.
Artigo em Inglês | MEDLINE | ID: mdl-27921105

RESUMO

Silver materials have been widely used as antimicrobial agents. Notably, silver nanoparticles have emerged as a new generation of nanoproducts for biomedical and environmental applications in recent years. However, ultrasmall silver nanoclusters (NCs) (∼2 nm) have rarely been used to kill bacteria and their antibacterial mechanisms have not yet been fully elucidated. Herein, we studied the antibacterial activities of bifunctional fluorescent DHLA-AgNCs against three types of bacteria. The results showed that DHLA-AgNCs exhibited excellent antibacterial activities against Gram-negative E. coli, which could efficiently inhibit the growth of E. coli DH 5α and E. coli DSM 4230 cells at a concentration of 15 and 10 µg mL-1, respectively. Meanwhile AgNCs demonstrated no apparent antibacterial activity against Gram-positive S. aureus. Then, the antibacterial mechanisms of AgNCs were systematically investigated. We found that AgNCs affected the growth of different E. coli strains in different ways. AgNCs inhibited the growth of E. coli DH 5α mainly through damaging the outer cellular membrane and permeating into the cells, followed by the antibacterial effect of the internalized AgNCs and released silver ions. AgNCs, however, inhibited the growth of E. coli DSM 4230 cells mainly through diffusing into E. coli DSM 4230 cells and damaging their respiratory chain. These results clearly indicated that different bacterial strains (e.g. different E. coli strains) should be taken into consideration in future studies. Our work facilitates further investigation of the design of new antibacterial silver nanomaterials with different sizes.


Assuntos
Antibacterianos/farmacologia , Escherichia coli/efeitos dos fármacos , Nanopartículas Metálicas/química , Prata/farmacologia , Staphylococcus aureus/efeitos dos fármacos , Antibacterianos/química , Relação Dose-Resposta a Droga , Escherichia coli/citologia , Escherichia coli/crescimento & desenvolvimento , Testes de Sensibilidade Microbiana , Tamanho da Partícula , Prata/química , Relação Estrutura-Atividade
16.
Mol Clin Oncol ; 5(5): 532-536, 2016 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-27900079

RESUMO

Multiple myeloma (MM) is characterized by abnormal proliferation of neoplastic plasma cells. Pleural effusion as an initial presentation of this disease is rare, as is true pleural myeloma. We herein present a case of solitary pleural myelomatous lesion in a 70-year-old male patient diagnosed by pleural biopsy via semi-rigid thoracoscopy followed by histopathological examination. Furthermore, a review of the related English literature identified 22 cases of pleural myeloma, only 3 of which were diagnosed by video-assisted thoracoscopy. To the best of our knowledge, this is the first reported case of a solitary pleural myelomatous lesion diagnosed by pleural biopsy via semi-rigid thoracoscopy. Patients with MM with pleural involvement, including the present case, appear to have a short survival despite aggressive treatment. Our patient received chemotherapy with bortezomib, epiadriamycin and dexamethasone; however, he deteriorated rapidly after one cycle of chemotherapy and succumbed to the disease 8 weeks after the initial presentation. According to our experience, semi-rigid thoracoscopy is an effective and safe method for obtaining a pleural specimen for histopathological evaluation.

17.
Molecules ; 21(11)2016 Nov 09.
Artigo em Inglês | MEDLINE | ID: mdl-27834877

RESUMO

Fuzi has been used to treat diabetic complications for many years in china. In a previous study, we have shown that Fuzi aqueous extract can attenuate Diabetic peripheral neuropathy (DPN) in rats and protect Schwann cells from injury. Thus, the protective effect of Fuzi polysaccharides (FPS) on high glucose-induced SCs and the preliminary mechanism were investigated. Firstly, the FPS were obtained and their monose composition was analyzed by the combination of pre-column derivatization and high performance liquid chromatography coupled with electrospray ionization multi-tandem mass spectrometry (HPLC/ESI-MSn). The results witnessed the efficiency of this method and seven monosaccharides were tentatively identified, among which fucose was first reported. Simultaneously, m/z 215 can be considered as diagnostic ions to confirm the number of monosaccharides. Next, high glucose-induced SC model was applied and divided into model group, treated group of FPS, normal and osmotic control group. After treatment for 48 h, the data showed FPS could significantly decrease the intracellular ROS and apoptosis, which were determined by the corresponding fluorescent probes. Then, the expression of oxidative stress-related proteins in SCs were measured by Western blot. Furthermore, the protein tests found that FPS markedly up-regulated superoxide dismutase (SOD), catalase (CAT) and peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) protein level, but down-regulated NADPH oxidase-1 (Nox1) protein level. Moreover, FPS could also increase AMP-activated protein kinase (AMPK) activation significantly. Hence, we preliminary deduced that AMPK-PGC-1α pathway may play an important role in the protective effect of FPS against high glucose-induced cell damage.


Assuntos
Neuropatias Diabéticas/tratamento farmacológico , Glucose/metabolismo , Polissacarídeos , Células de Schwann/metabolismo , Animais , Configuração de Carboidratos , Linhagem Celular , Cromatografia Líquida de Alta Pressão , Neuropatias Diabéticas/metabolismo , Neuropatias Diabéticas/patologia , Espectrometria de Massas , Estresse Oxidativo/efeitos dos fármacos , Oxirredutases/biossíntese , Coativador 1-alfa do Receptor gama Ativado por Proliferador de Peroxissomo/biossíntese , Extratos Vegetais/química , Extratos Vegetais/farmacologia , Polissacarídeos/química , Polissacarídeos/farmacologia , Ratos , Espécies Reativas de Oxigênio/metabolismo , Células de Schwann/patologia
18.
BMC Ophthalmol ; 16(1): 158, 2016 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-27590038

RESUMO

BACKGROUND: Reis-Bücklers corneal dystrophy (RBCD) was consistently reported as a corneal dystrophy only affected Bowman's layer and superficial corneal stroma, and superficial keratectomy was a recommendation surgery for treatment in literatures. The study reported new histopathological and ultrastructural findings in RBCD caused by the Arg124Leu mutation of transforming growth factor induced (TGFBI) gene in a four-generation Chinese pedigree. METHODS: Subjects including eight patients and seven unaffected family members received slit-lamp biomicroscopy and photography. DNA was obtained from all subjects, and exons 4 and 11 to 14 of TGFBI gene were analyzed by polymerase chain reaction and the products were sequenced. Anterior segment optical coherence tomography (AS OCT) and in vivo confocal microscopy were conducted for ten eyes of five patients. Based on the results of AS OCT and in vivo confocal microscopy, deep anterior lamellar keratoplasty (DLKP) using cryopreserved donor cornea was applied for four eyes of four patients. Four lamellar dystrophic corneal buttons were studied by light and transmission electron microscopy, and TGFBI immunohistochemistry. RESULTS: Eight patients had typical clinical manifestations of RBCD presenting recurrent painful corneal erosion starting in their early first decades, along with age-dependent progressive geographic corneal opacities. TGFBI sequencing revealed a heterozygous mutation, Arg124Leu in all eight patients. Anterior segment optical coherence tomography and in vivo confocal microscopy showed the dystrophic deposits involved not only in subepithelial and superficial stroma, but also in mid- or posterior stroma in four examined advanced eyes. Light microscopy showed Bowman's layer was absent, replaced by abnormal deposits stain bright red with Masson's trichrome. In superficial cornea, the deposits stacked and produced three to five continuous bands parallel to the corneal collagen lamellae. In mid- to posterior stroma, numerous granular or dot- like aggregates were heavily scattered, and most of them presented around the nuclei of stromal keratocytes. Transmission electron microscopy revealed the multiple electron-dense rod-shaped deposits aggregated and formed a characteristic pattern of three to five continuous bands in superficial cornea, which were similar to those seen under light microscopy. In mid- to posterior stroma, clusters of rod-shaped bodies were scattered extracellular or intracellular of the stromal keratocytes between the stromal lamellae suggesting the close relationship between mutated proteins and keratocyte. CONCLUSIONS: The study offer evidences indicating DLKP is a viable treatment option for advanced RBCD to avoid recurrence, and the mutated TGFBIp in dystrophic corneas are of keratocytes origin.


Assuntos
Distrofias Hereditárias da Córnea/genética , Distrofias Hereditárias da Córnea/patologia , Fator de Crescimento Transformador beta/genética , Adolescente , Adulto , Idoso , Grupo com Ancestrais do Continente Asiático , Lâmina Limitante Anterior/patologia , Lâmina Limitante Anterior/ultraestrutura , Criança , Pré-Escolar , Córnea/patologia , Córnea/ultraestrutura , Distrofias Hereditárias da Córnea/cirurgia , Ceratócitos da Córnea/patologia , Substância Própria/patologia , Substância Própria/ultraestrutura , Éxons , Proteínas da Matriz Extracelular/genética , Feminino , Humanos , Imuno-Histoquímica , Ceratoplastia Penetrante , Masculino , Microscopia Confocal , Microscopia Eletrônica de Transmissão , Pessoa de Meia-Idade , Mutação , Linhagem , Reação em Cadeia da Polimerase , Tomografia de Coerência Óptica , Adulto Jovem
19.
Chem Commun (Camb) ; 52(77): 11579-82, 2016 Oct 04.
Artigo em Inglês | MEDLINE | ID: mdl-27605263

RESUMO

A highly sensitive and selective Rhodamine B-based fluorescent sensor, RhB-1, for glutathione (GSH) was easily synthesized. An extremely fast detection response time of 10 s, which is the fastest one ever reported, is achieved in aqueous solutions over a wide pH range with large enhancement of emission intensity. The sensor detects GSH in cells and selectively accumulates in lysosomes.

20.
Toxins (Basel) ; 8(2): 52, 2016 Feb 20.
Artigo em Inglês | MEDLINE | ID: mdl-26907346

RESUMO

Insect host/parasitoid interactions are co-evolved systems in which host defenses are balanced by parasitoid mechanisms to disable or hide from host immune effectors. Here, we report that Pteromalus puparum venom impairs the antimicrobial activity of its host Pieris rapae. Inhibition zone results showed that bead injection induced the antimicrobial activity of the host hemolymph but that venom inhibited it. The cDNAs encoding cecropin and lysozyme were screened. Relative quantitative PCR results indicated that all of the microorganisms and bead injections up-regulated the transcript levels of the two genes but that venom down-regulated them. At 8 h post bead challenge, there was a peak in the transcript level of the cecropin gene, whereas the peak of lysozyme gene occurred at 24 h. The transcripts levels of the two genes were higher in the granulocytes and fat body than in other tissues. RNA interference decreased the transcript levels of the two genes and the antimicrobial activity of the pupal hemolymph. Venom injections similarly silenced the expression of the two genes during the first 8 h post-treatment in time- and dose-dependent manners, after which the silence effects abated. Additionally, recombinant cecropin and lysozyme had no significant effect on the emergence rate of pupae that were parasitized by P. puparum females. These findings suggest one mechanism of impairing host antimicrobial activity by parasitoid venom.


Assuntos
Borboletas/efeitos dos fármacos , Borboletas/parasitologia , Regulação da Expressão Gênica/efeitos dos fármacos , Interações Hospedeiro-Parasita , Venenos de Vespas/toxicidade , Vespas/fisiologia , Animais , Borboletas/genética , Borboletas/imunologia , Hemolinfa/imunologia , Imunidade Humoral/efeitos dos fármacos , Proteínas de Insetos/genética , Muramidase/genética , Pupa/efeitos dos fármacos , Pupa/genética , Pupa/imunologia , Pupa/parasitologia
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA