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1.
Mil Med Res ; 9(1): 51, 2022 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-36131323

RESUMO

BACKGROUND: Tumor cell heterogeneity mediated drug resistance has been recognized as the stumbling block of cancer treatment. Elucidating the cytotoxicity of anticancer drugs at single-cell level in a high-throughput way is thus of great value for developing precision therapy. However, current techniques suffer from limitations in dynamically characterizing the responses of thousands of single cells or cell clones presented to multiple drug conditions. METHODS: We developed a new microfluidics-based "SMART" platform that is Simple to operate, able to generate a Massive single-cell array and Multiplex drug concentrations, capable of keeping cells Alive, Retainable and Trackable in the microchambers. These features are achieved by integrating a Microfluidic chamber Array (4320 units) and a six-Concentration gradient generator (MAC), which enables highly efficient analysis of leukemia drug effects on single cells and cell clones in a high-throughput way. RESULTS: A simple procedure produces 6 on-chip drug gradients to treat more than 3000 single cells or single-cell derived clones and thus allows an efficient and precise analysis of cell heterogeneity. The statistic results reveal that Imatinib (Ima) and Resveratrol (Res) combination treatment on single cells or clones is much more efficient than Ima or Res single drug treatment, indicated by the markedly reduced half maximal inhibitory concentration (IC50). Additionally, single-cell derived clones demonstrate a higher IC50 in each drug treatment compared to single cells. Moreover, primary cells isolated from two leukemia patients are also found with apparent heterogeneity upon drug treatment on MAC. CONCLUSION: This microfluidics-based "SMART" platform allows high-throughput single-cell capture and culture, dynamic drug-gradient treatment and cell response monitoring, which represents a new approach to efficiently investigate anticancer drug effects and should benefit drug discovery for leukemia and other cancers.


Assuntos
Leucemia , Microfluídica , Células Clonais , Clonagem Molecular , Detecção Precoce de Câncer , Humanos , Mesilato de Imatinib , Microfluídica/métodos , Resveratrol
2.
Front Plant Sci ; 13: 828153, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36119623

RESUMO

Soybean proteins are limited by their low contents of methionine and cysteine. Herein, 7S globulin accumulation was reduced using RNA interference to silence CG-ß-1 expression, and the content of the A2B1a subunit was largely increased under the soybean seed-specific oleosin8 promoter. The results showed that the sulfur-containing amino acid content in soybean seeds drastically improved, reaching 79.194 nmol/mg, and the 11S/7S ratio had a 1.89-fold increase compared to the wild-type acceptor. The secondary structures of 11S globulin were also altered, and the ß-sheet content increased with decreasing ß-turn content, which was confirmed by Fourier transform infrared spectroscopy, Raman spectroscopy and circular dichroism analysis. Our findings suggested that raising the accumulation of 11S glycinin at the expense of reducing the content of 7S globulin is an attractive and precise engineering strategy to increase the amount of sulfur-containing amino acids, and soybean proteins with A2B1a subunits of 11S isolates improved, and ß-subunits of 7S fractions reduced simultaneously might be an effective new material for food production.

3.
Dalton Trans ; 2022 Sep 07.
Artigo em Inglês | MEDLINE | ID: mdl-36069318

RESUMO

The preparation of low-cost and high-activity oxygen evolution reaction (OER) catalysts is a technical bottleneck in the field of electrolysis of water to produce hydrogen. Amorphous metal-organic frameworks (MOFs) with low-cost transition metals have attracted increasing attention in the catalytic field, yet metal atoms that are the main active sites are still ambiguous. Here, we synthesized a series of amorphous ternary Fex(NiCu)3-x-MOFs via an ultrasonic method. The optimal amorphous FeNiCu-MOF is found to be able to supply a current density of 10 mA cm-2 merely at a low overpotential of 260 mV with a small Tafel slope of 61 mV dec-1 and exhibits high durability over 24 h. Moreover, density functional theory (DFT) calculations show that the Fe atoms are the main active sites for the OER in the FeNiCu-MOF. This work shows that amorphous ternary MOFs have great potential for application in OER electrocatalysts due to the multiple synergistic effects and amorphous MOF structures.

4.
Polymers (Basel) ; 14(18)2022 Sep 08.
Artigo em Inglês | MEDLINE | ID: mdl-36145906

RESUMO

In this work, the thermogravimetry-Fourier transform infrared spectroscopy (TG-FTIR) and gas chromatography-mass spectrometry (GC-MS) techniques are used to investigate the thermal degradation behavior of polypropylene (PP) with 20 wt.% diatomite (DM). The initial decomposition temperature of these blends was 17 °C lower than that of pristine PP, and more olefin degradation products were formed during the pyrolysis process under Ar atmosphere. These results could be attributed to the catalytic effects of DM on the degradation of PP and the changes of PP chain scission pathways around the particles (more ß scission happened via the secondary radical transfer). These olefins could be caught by DM through the Si-O-C bond formed during the heat-treatment around 400~500 °C. The formation of the cross-linked structure could facilitate the growth of graphene during a high-temperature graphitization process.

5.
Front Pharmacol ; 13: 961941, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36091799

RESUMO

Osteoporosis is called a silent disease, because it is difficult to detect until comprehensive examinations for osteoporosis are performed or osteoporotic fractures occur. Zoledronic acid is currently the first-line anti-osteoporotic drug, with good efficacy and treatment compliance. A major advantage of zoledronic acid is that intravenous zoledronic acid often guarantees a therapeutic effect for up to 1 year after infusion. The reasons why zoledronic acid is effective in improving osteoporosis are that it can inhibit osteoclast differentiation and induce osteoclast apoptosis, thus suppressing bone resorption and increasing bone density. The story between zoledronic acid and osteoclasts has been written long time ago. Both the canonical receptor activator of the receptor activator of nuclear factor-κB ligand (RANKL) pathway and the non-canonical Wnt pathway are the main pathways by which zoledronic acid inhibits osteoclast differentiation. Farnesyl pyrophosphate synthase (FPPS), reactive oxygen species (ROS), and ferroptosis that was first proposed in 2012, are all considered to be closely associated with zoledronic acid-induced osteoclast apoptosis. Here, we provide a brief review of the recent progress on the study of zoledronic acid and osteoclasts, and hope to elaborate how zoledronic acid improves osteoporosis by acting on osteoclasts.

6.
J Med Chem ; 65(18): 12176-12187, 2022 Sep 22.
Artigo em Inglês | MEDLINE | ID: mdl-36066386

RESUMO

Targeted degradation of proteins, especially those regarded as undruggable or difficult to drug, attracts wide attention to develop novel therapeutic strategies. Glutathione peroxidase 4 (GPX4), the key enzyme regulating ferroptosis, is currently a target with just covalent inhibitors. Here, we developed a targeted photolysis approach and achieved efficient degradation of GPX4. The photodegradation-targeting chimeras (PDTACs) were synthesized by conjugating a clinically approved photosensitizer (verteporfin) to noninhibitory GPX4-targeting peptides. These chimeras selectively degraded the target protein in both cell lysates and living cells upon red-light irradiation. The targeted photolysis of GPX4 resulted in dominant ferroptotic cell death in malignant cancer cells. Moreover, the dying cells resulting from the PDTACs exhibited potent immunogenicity in vitro and efficiently elicited antitumor immunity in vivo. Our approach therefore provides a novel method to induce GPX4 dysfunction based on noncovalent binding and specifically trigger immunogenic ferroptosis, which may boost the application of ferroptosis in cancer immunotherapy.


Assuntos
Ferroptose , Fosfolipídeo Hidroperóxido Glutationa Peroxidase , Fotólise , Fármacos Fotossensibilizantes , Verteporfina
7.
J Clin Transl Hepatol ; 10(4): 680-691, 2022 Aug 28.
Artigo em Inglês | MEDLINE | ID: mdl-36062271

RESUMO

Background and Aims: Radiation-induced liver fibrosis (RILF), delayed damage to the liver (post-irradiation) remains a major challenge for the radiotherapy of liver malignancies. This study investigated the potential function and mechanism of circTUBD1 in the development of RILF. Methods: By using a dual luciferase assay, RNA pull-down assays, RNA sequencing, chromatin immunoprecipitation (known as ChIP) assays, and a series of gain- or loss-of-function experiments, it was found that circTUBD1 regulated the activation and fibrosis response of LX-2 cells induced by irradiation via a circTUBD1/micro-203a-3p/Smad3 positive feedback loop in a 3D system. Results: Knockdown of circTUBD1 not only reduced the expression of α-SMA, as a marker of LX-2 cell activation, but also significantly decreased the levels of hepatic fibrosis molecules, collagen type I alpha 1 (COL1A1), collagen type III alpha 1 (COL3A1), and connective tissue growth factor (CTGF) in a three-dimensional (3D) culture system and RILF model in vivo. Notably, knockdown of circTUBD1 alleviated early liver fibrosis induced by irradiation in mice models. Conclusions: This study is the first to reveal the mechanism and role of circTUBD1 in RILF via a circTUBD1/micro-203a-3p/Smad3 feedback loop, which provides a novel therapeutic strategy for relieving the progression of RILF.

8.
Artigo em Inglês | MEDLINE | ID: mdl-36078801

RESUMO

Xinfengjiang River, the largest tributary of Dongjiang River, plays a key role in the water supply of Heyuan, Huizhou, Guangzhou and even the Pearl River urban agglomeration. It is crucial to determine the pollution status, potential ecological risk degree of heavy metals in Xinfengjiang river sediment and their influence on the abundance of fish species. In this paper, seven heavy metal concentrations in sediment from the Heyuan section of the Xinfengjiang river were investigated. The order of average concentration was: As > Zn > Pb > Cr > Cu > Cd > Hg. The average concentrations of Cd, Zn and Cu in the upper reaches of the Xinfengjiang Reservoir were significantly higher than those in the reservoir. The mean value order of Igeo was: Cd > Zn > Pb > As > Cu > Cr > Hg. Cd and As had the highest ecological risk index and the greatest threat to the ecological environment. Pearson correlation analysis and principal component analysis demonstrated that the pollution source of heavy metals such as Cu and Cd are much more likely to originate from the mine fields located in the northeast of the sampling sites. In addition, agriculture, electronic industry and domestic sewage also contributed to the concentration of heavy metals in different degrees. Redundancy analysis showed that the abundance of Cypriniformes was negatively correlated with Cu and Cd concentrations, suggesting that mining activities might indirectly affect the abundance of fish species.


Assuntos
Mercúrio , Metais Pesados , Poluentes Químicos da Água , Cádmio/análise , China , Monitoramento Ambiental , Sedimentos Geológicos/análise , Chumbo/análise , Mercúrio/análise , Metais Pesados/análise , Medição de Risco , Rios , Poluentes Químicos da Água/análise
9.
Sensors (Basel) ; 22(17)2022 Aug 30.
Artigo em Inglês | MEDLINE | ID: mdl-36080984

RESUMO

Entire surface point clouds in complex objects cannot be captured in a single direction by using noncontact measurement methods, such as machine vision; therefore, different direction point clouds should be obtained and registered. However, high efficiency and precision are crucial for registration methods when dealing with huge number of point clouds. To solve this problem, an improved registration algorithm based on double threshold feature extraction and distance disparity matrix (DDM) is proposed in this study. Firstly, feature points are extracted with double thresholds using normal vectors and curvature to reduce the number of points. Secondly, a fast point feature histogram is established to describe the feature points and obtain the initial corresponding point pairs. Thirdly, obviously wrong corresponding point pairs are eliminated as much as possible by analysing the Euclidean invariant features of rigid body transformation combined with the DDM algorithm. Finally, the sample consensus initial alignment and the iterative closest point algorithms are used to complete the registration. Experimental results show that the proposed algorithm can quickly process large data point clouds and achieve efficient and precise matching of target objects. It can be used to improve the efficiency and precision of registration in distributed or mobile 3D measurement systems.

10.
Hematology ; 27(1): 994-1002, 2022 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-36066283

RESUMO

INTRODUCTION: Several studies have confirmed that mutations in the Wilms tumor 1 (WT1) gene occur in adult acute myeloid leukemia (AML). However, few data are available regarding the incidence of WT1 mutations in CEBPAmut AML and their impact. METHODS: We retrospectively analyzed the frequency and clinical impact of WT1 mutations in 220 newly diagnosed AML patients with CEBPA mutations(CEBPAmut). Chromosome karyotype analysis was performed by R or G banding method and further confirmed either by fluorescence in situ hybridization (FISH) and/or by multiple reverse transcription polymerase chain reaction (multiple RT-PCR). Mutations were detected with a panel of 112mutational genes using next-generation sequencing (NGS). RESULTS: Overall, 30 WT1 mutations were detected in 29 of the 220 CEBPAmut AML patients (13.18%) screened. These mutations clustered overwhelmingly in exon 7 (n=16). WT1 mutations were found to be significantly more frequent in AML patients with double-mutated CEBPA (CEBPAdm) than in AML patients with single-mutated CEBPA (17.36%vs. 8.08%, P = 0.043). Among WT1-mutated patients, the most common co-mutation was FLT3-ITD (n = 7, 24.14%), followed by NRAS (n = 5, 17.24%), CSF3R (n = 4, 13.79%), GATA2 (n = 4, 13.79%), and KIT (n = 4, 13.79%). The most frequent functional pathway was signaling pathways inas many as 62.07% of cases. Notably,the concomitant mutations in epigenetic regulatorswere inversely correlated with WT1 mutations(P = 0.003). CEBPAdm AML patients with WT1 mutations had inferior relapse-free survival, event-free survival and overall survival compared with patients CEBPAdm AML without WT1 mutations (P = 0.002, 0.004, and 0.010, respectively). CONCLUSION: Our data showed that WT1 mutations are frequently identified in CEBPAmut AML, especially in CEBPAdm AML. CEBPAmut AML patients with WT1 mutations show distinct spectrum of comutations. In the context of CEBPAdm AML, WT1 mutations predict a poor prognosis.


Assuntos
Leucemia Mieloide Aguda , Proteínas Estimuladoras de Ligação a CCAAT/genética , Humanos , Hibridização in Situ Fluorescente , Leucemia Mieloide Aguda/patologia , Mutação , Prognóstico , Estudos Retrospectivos , Proteínas WT1/genética
11.
Gut Microbes ; 14(1): 2119055, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-36093568

RESUMO

Studies of the gut-liver axis have enhanced our understanding of the pathophysiology of various liver diseases and the mechanisms underlying the regulation of the effectiveness of therapies. Radiotherapy (RT) is an important therapeutic option for patients with unresectable hepatocellular carcinoma (HCC). However, the role of the microbiome in regulating the response to RT remains unclear. The present study characterizes the gut microbiome of patients responsive or non-responsive to RT and investigates the molecular mechanisms underlying the differences in patient response. We collected fecal samples for 16S rRNA sequencing from a prospective longitudinal trial of 24 HCC patients receiving RT. We used fecal microbiota transplantation (FMT), flow cytometry, and transcriptome sequencing to explore the effects of dysbiosis on RT. We also examined the role of stimulator of interferon genes (STING) in RT-associated antitumor immune responses mediated by gut microbiota in STING- (Tmem173-/-) and cGAS-knockout (Mb21d1-/-) mouse models. We propose that primary resistance to RT could be attributed to the disruption of the gut microbiome. Mechanistically, gut microbiome dysbiosis impairs antitumor immune responses by suppressing antigen presentation and inhibiting effector T cell functions through the cGAS-STING-IFN-I pathway. Cyclic-di-AMP - an emerging second messenger of bacteria - may act as a STING agonist and is thus a potential target for the prediction and modulation of responses to RT in HCC patients. Our study highlights the therapeutic potential of modulating the gut microbiome in HCC patients receiving RT and provides a new strategy for the radiosensitization of liver cancer.


Assuntos
Carcinoma Hepatocelular , Microbioma Gastrointestinal , Neoplasias Hepáticas , Animais , Carcinoma Hepatocelular/radioterapia , Disbiose/terapia , Imunidade , Neoplasias Hepáticas/radioterapia , Camundongos , Nucleotidiltransferases/metabolismo , Estudos Prospectivos , RNA Ribossômico 16S/genética
12.
Int Immunopharmacol ; 111: 109116, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35969899

RESUMO

Vasculogenic mimicry (VM), a vessel-like structure independent of endothelial cells, commonly exists in solid tumors which requires blood vessels to grow. As a special source of blood supply for tumor progression to a more aggressive state, VM has been observed in a variety of human malignant tumors and is tightly associated with tumor proliferation, invasion, metastasis, and poor patient prognosis. So far, various factors, including immune cells and cytokines, were reported to regulate ovarian cancer progression by influencing VM formation. Herein, we review the mechanisms that regulate VM formation in ovarian cancer and the effect of cells, cytokines, and signaling molecules in the tumor microenvironment on VM formation, Furthermore, we summarize the current clinical application of drugs targeting VM formation.


Assuntos
Neoplasias Ovarianas , Microambiente Tumoral , Linhagem Celular Tumoral , Citocinas/uso terapêutico , Células Endoteliais/patologia , Feminino , Humanos , Imunoterapia , Neovascularização Patológica/tratamento farmacológico , Neoplasias Ovarianas/tratamento farmacológico
13.
Reprod Domest Anim ; 2022 Aug 25.
Artigo em Inglês | MEDLINE | ID: mdl-36005750

RESUMO

We investigated the structural features of arterial blood vessels in yak uterine caruncle and the effects of the expression of vascular regulation-related factors on angiogenesis in pregnant and non-pregnant yak uterus. Three-dimensional specimens of the uterine artery of non-pregnant and pregnant yaks were produced to observe and measure the distribution characteristics and number of arterial vessels in the uterus and caruncle in the two periods. The uterine caruncle structure was observed and analysed by haematoxylin-eosin staining. The expression features of vascular endothelial growth factor (VEGF) and angiopoietin-1 (Ang-1) in the uterine caruncle were detected with immunohistochemistry, quantitative real-time PCR (qRT-PCR) and western blotting. The length and number of blood vessels in the caruncle were increased, the degree of curvature was decreased, and the folding was more complicated during pregnancy as compared with that during non-pregnancy. The immunohistochemical results demonstrated that VEGF and Ang-1 were mainly expressed strongly in the mucosal epithelial cytoplasm. The glandular lumen of the uterine gland, lymphocytes and the media and adventitia of blood vessels are widely distributed, and they are all positive. VEGF and Ang-1 mRNA and protein levels were highest in pregnancy, followed by that in the luteal phase and in the follicular phase, and three stages were significantly different (p < .05). These findings provide an anatomical reference and theoretical basis for improving the diagnosis and treatment of yak reproductive disorders and other diseases in high-altitude and low-oxygen environments.

14.
ChemSusChem ; : e202201352, 2022 Aug 24.
Artigo em Inglês | MEDLINE | ID: mdl-36000791

RESUMO

The practical applications of lithium metal anodes have been severely hindered by the Li dendrite issue. Herein, a dual structure-material design strategy was developed to fabricate a new type of separator using interconnected hollow porous polyacrylonitrile (PAN) nanofibers (HPPANF), which delivered controllable and dendrite-free Li depositions. The interconnected mesopores on HPPANF bridged the hollow interiors with the outside voids among the fibers, enabling outstanding electrolyte uptake capabilities for high ion conductivity, and nano-level wetted electrolyte/anode interface for uniform Li plating/stripping. In parallel, the HPPANF separator enriched with polar groups acted as an exceptional polymer-based solid-state electrolyte, providing 3D ion channels for the transport of Li ions. Benefiting from the dual structure-material design, the HPPANF separator induced uniform Li ion flux for dendrite-free Li depositions, which caused enhanced cycling stability (1300 h, 3 mA cm-2 ). This work demonstrates a new method to stabilize Li metal anodes through rational separator design.

15.
Bioelectrochemistry ; 147: 108217, 2022 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-35930950

RESUMO

Nanomaterials play an important role in the fields of electrocatalysis and electrochemical immunosensor. As we all know, electrocatalytic oxygen evolution reaction (OER) can generate intensive current response under alkaline and even neutral conditions, and no by-products are produced. In recent years, OER based on non-noble metal nanocatalysts has become a research focus in the field of new energy, but there are few reports on using it as an electrocatalytic immunosensor label for biomolecule detection. In this study, we successfully synthesized CoFe-(oxy)hydroxide nanosheets through transforming CoFe prussian blue analogue (CoFe PBA). Based on the excellent OER activity of CoFe-(oxy)hydroxide in neutral solution, it was used as a new electrocatalytic marker for capturing procalcitonin (PCT) secondary antibody (Ab2). Meanwhile, gold nanoparticles (Au NPs) modified glassy carbon electrode (GCE) was utilized as immunosensor platform to immobilize PCT primary antibodies (Ab1). The linear range of the immunosensor for PCT detection is 0.0005 âˆ¼ 100 ng mL-1, and the detection limit is 0.33 pg mL-1. Furthermore, a high recovery was obtained when this proposed method was applied to analysis human serum samples. This work not only provides a new method for clinical analysis of PCT, but also extends the application of electrocatalytic OER.


Assuntos
Técnicas Biossensoriais , Nanopartículas Metálicas , Anticorpos , Técnicas Biossensoriais/métodos , Técnicas Eletroquímicas/métodos , Ouro/química , Humanos , Hidróxidos , Imunoensaio/métodos , Limite de Detecção , Nanopartículas Metálicas/química , Oxigênio , Pró-Calcitonina
16.
Phys Rev E ; 106(1-1): 014611, 2022 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-35974635

RESUMO

Collective decision making in a biological motion group requires fast and robust transmission of information. Typically, directional switching information propagation across the whole group obeys a linear dispersion law. However, conventional dynamic collective motion models, such as the Vicsek model and the Couzin model did not take into account ultrafast directional synchronous motions. In the present paper, a multiparticle model is proposed based on inertial spin self-propel action, which can provide adequate description of such group motion. By considering both spin mechanism and collision avoidance, the proposed self-propelled particle spin model can nicely describe collective motion with fast directional switching. By analyzing the order parameter of the group-velocity synchronization, a mechanism of group decision making is revealed, which is based on the difference between two clusters of divergent leaders, showing a transition from the compromising phase (i.e., following the group average) to the preferred phase (i.e., aligning to a leader cluster). The finding provides new insight to the decision-making process of followers when they face with divergent leaders in group motion.

17.
Microbiol Spectr ; : e0160222, 2022 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-35972246

RESUMO

The therapeutic use of bacteriophages (phages) provides great promise for treating multidrug-resistant (MDR) bacterial infections. However, an incomplete understanding of the interactions between phages and bacteria has negatively impacted the application of phage therapy. Here, we explored engineered anti-CRISPR (Acr) gene-containing phages (EATPs, eat Pseudomonas) by introducing Type I anti-CRISPR (AcrIF1, AcrIF2, and AcrIF3) genes into the P. aeruginosa bacteriophage DMS3/DMS3m to render the potential for blocking P. aeruginosa replication and infection. In order to achieve effective antibacterial activities along with high safety against clinically isolated MDR P. aeruginosa through an anti-CRISPR immunity mechanism in vitro and in vivo, the inhibitory concentration for EATPs was 1 × 108 PFU/mL with a multiplicity of infection value of 0.2. In addition, the EATPs significantly suppressed the antibiotic resistance caused by a highly antibiotic-resistant PA14 infection. Collectively, these findings provide evidence that engineered phages may be an alternative, viable approach by which to treat patients with an intractable bacterial infection, especially an infection by clinically MDR bacteria that are unresponsive to conventional antibiotic therapy. IMPORTANCE Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic Gram-negative bacterium that causes severe infection in immune-weakened individuals, especially patients with cystic fibrosis, burn wounds, cancer, or chronic obstructive pulmonary disease (COPD). Treating P. aeruginosa infection with conventional antibiotics is difficult due to its intrinsic multidrug resistance. Engineered bacteriophage therapeutics, acting as highly viable alternative treatments of multidrug-resistant (MDR) bacterial infections, have great potential to break through the evolutionary constraints of bacteriophages to create next-generation antimicrobials. Here, we found that engineered anti-CRISPR (Acr) gene-containing phages (EATPs, eat Pseudomonas) display effective antibacterial activities along with high safety against clinically isolated MDR P. aeruginosa through an anti-CRISPR immunity mechanism in vitro and in vivo. EATPs also significantly suppressed the antibiotic resistance caused by a highly antibiotic-resistant PA14 infection, which may provide novel insight toward developing bacteriophages to treat patients with intractable bacterial infections, especially infections by clinically MDR bacteria that are unresponsive to conventional antibiotic therapy.

18.
Sensors (Basel) ; 22(15)2022 Aug 05.
Artigo em Inglês | MEDLINE | ID: mdl-35957407

RESUMO

Spherical targets are widely used in coordinate unification of large-scale combined measurements. Through its central coordinates, scanned point cloud data from different locations can be converted into a unified coordinate reference system. However, point cloud sphere detection has the disadvantages of errors and slow detection time. For this reason, a novel method of spherical object detection and parameter estimation based on an improved random sample consensus (RANSAC) algorithm is proposed. The method is based on the RANSAC algorithm. Firstly, the principal curvature of point cloud data is calculated. Combined with the k-d nearest neighbor search algorithm, the principal curvature constraint of random sampling points is implemented to improve the quality of sample points selected by RANSAC and increase the detection speed. Secondly, the RANSAC method is combined with the total least squares method. The total least squares method is used to estimate the inner point set of spherical objects obtained by the RANSAC algorithm. The experimental results demonstrate that the method outperforms the conventional RANSAC algorithm in terms of accuracy and detection speed in estimating sphere parameters.


Assuntos
Algoritmos , Consenso , Análise dos Mínimos Quadrados
19.
J Food Biochem ; : e14382, 2022 Aug 26.
Artigo em Inglês | MEDLINE | ID: mdl-36017617

RESUMO

Methylglyoxal (MGO), a cytotoxic byproduct of glycolysis, causes neuro oxidative damage and apoptosis, and plays key roles in diabetic encephalopathy (DE). The goal of this research was to evaluate the roles of lutein attenuated MGO-induced damage in PC12 cells as well as the underlying mechanisms. The findings of this study showed that lutein has a significant impact on reducing the generation of reactive oxygen species (ROS) and oxidative stress in MGO-induced PC12 cells, which may be attributed to the increased antioxidant enzymes activity and the decreased MDA levels. Moreover, treatment with lutein also alleviated cell apoptosis and mitochondrial damage. Real-time PCR and western blot analysis showed that lutein enhanced the Bcl-2:Bax ratio, inhibited the expression of caspase-3 and caspase-9, and increased the protein level of phosphorylated Akt. The network pharmacology and molecular docking prediction results suggested that the phosphoinositide 3-kinase/protein kinase B (PI3K/Akt) signaling pathway was a potential mechanism of lutein in DE treatment. Furthermore, LY294002, a specific PI3K inhibitor, partially abolished the protective effect of lutein. These results presented that lutein attenuated oxidative damage and apoptosis triggered by MGO in PC12 cells via the PI3K/Akt signaling pathway. PRACTICAL APPLICATIONS: Lutein is a common carotenoid dispersed in fruits and vegetables. This article confirmed a protective effect of lutein on oxidative damage and apoptosis in PC12 cells after MGO damage. These results indicated that lutein could potentially be developed as a nutraceutical or functional food in the prevention of diabetic-related neurodegenerative diseases.

20.
Nano Res ; 15(9): 7986-7993, 2022.
Artigo em Inglês | MEDLINE | ID: mdl-35855867

RESUMO

H2O2 is an environmentally friendly chemical for a wide range of water treatments. The industrial production of H2O2 is an anthraquinone oxidation process, which, however, consumes extensive energy and produces pollution. Here we report a green and sustainable piezocatalytic intermediate water splitting process to simultaneously obtain H2O2 and H2 using single crystal vanadium (V)-doped NaNbO3 (V-NaNbO3) nanocubes as catalysts. The introduction of V improves the specific surface area and active sites of NaNbO3. Notably, V-NaNbO3 piezocatalysts of 10 mg exhibit 3.1-fold higher piezocatalytic efficiency than the same catalysts of 50 mg, as more piezocatalysts lead to higher probability of aggregation. The aggregation causes reducing active sites and decreased built-in electric field due to the neutralization between different nano-catalysts. Remarkably, piezocatalytic H2O2 and H2 production rates of V-NaNbO3 (10 mol%) nanocubes (102.6 and 346.2 µmol·g-1·h-1, respectively) are increased by 2.2 and 4.6 times compared to the as-prepared pristine NaNbO3 counterparts, respectively. This improved catalytic efficiency is attributed to the promoted piezo-response and more active sites of NaNbO3 catalysts after V doping, as uncovered by piezo-response force microscopy (PFM) and density functional theory (DFT) simulation. More importantly, our DFT results illustrate that inducing V could reduce the dynamic barrier of water dissociation over NaNbO3, thus enhancing the yield of H2O2 and H2. This facile yet robust piezocatalytic route using minimal amounts of catalysts to obtain H2O2 and H2 may stand out as a promising candidate for environmental applications and water splitting. Electronic Supplementary Material: Supplementary material (typical Raman spectra of NaNbO3 and V-NaNbO3 with various doping concentrations (Fig. S1). XPS spectra of Na 1s (Fig. S2). PL spectra of solution obtained from the piezocatalytic system using NaNbO3 and V-NaNbO3 (10 mol%) as the catalysts after 1 h (Fig. S3). The length of NaNbO3 and V-NaNbO3 nanocubes calculated from XRD data of their (101) planes (Table S1)) is available in the online version of this article at 10.1007/s12274-022-4506-0.

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