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1.
J Colloid Interface Sci ; 581(Pt A): 21-30, 2021 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-32768732

RESUMO

Lanthanide ion (Ln3+)-doped nanoscale hydroxyapatites (nHAp) with tunable luminescence have attracted increasing attention due to their potential applications as useful biomedical tools (e.g., imaging and clinical therapy). In this study, we reported that doping Terbium (III) ions (Tb3+) in self-activated luminescent nHAp via a facile hydrothermal reaction, using trisodium citrate (Cit3-), generates unique emission-tunable probes known as Cit/Tb-nHAp. The morphology, crystal phase, and luminescence properties of these Cit/Tb-nHAp probes are studied in detail. Moreover, the results demonstrate that the luminescence of self-activated nHAp originates from the carbon dots trapped within the nHAp crystals, in which partial energy transfer occurs from carbon dots (CDs) to Tb3+. The color tunability is successfully achieved by regulating the addition of Cit3-. Biocompatibility study indicates that when co-cultured with C6 glioma cells in vitro for 3 days, ≤800 ppm Cit/Tb-nHAp is not cytotoxic for C6 glioma cells. We also present in vitro data showing efficient cytoplasmic localization of transferrin conjugated Cit/Tb-nHAp into C6 glioma cells by fluorescence cell imaging. We have successfully engineered Cit/Tb-nHAp, a promising biocompatible agent for future in vitro and in vivo fluorescence bioimaging.

2.
Artigo em Inglês | MEDLINE | ID: mdl-32627895

RESUMO

Given that many people suffer from large-area skin damage, skin regeneration is a matter of high concern. Here, an available method is developed for the formation of large-area robust skins through three stages: fabrication of a biodegradable sealant-loaded nanofiber scaffold (SNS), skin tissue reconstruction, and skin regeneration. First, a microfluidic blow-spinning strategy is proposed to fabricate a large-scale nanofiber scaffold with an area of 140 cm × 40 cm, composed of fibrinogen-loaded polycaprolactone/silk fibroin (PCL/SF) ultrafine core-shell nanofibers with mean diameter of 65 nm. Then, the SNS forms, where the gelling reaction of fibrin sealant occurs in situ between thrombin and fibrinogen on PCL/SF nanofiber surface, to promote the migration and proliferation of fibroblasts, accelerating skin regeneration. Through an in vivo study, it is shown that the SNS can rapidly repair acute tissue damage such as vascular bleeding and hepatic hemorrhage, and also promote angiogenesis, large-area abdominal wall defect repair, and wound tissue regeneration for medical problems in the world. Besides, it avoids the risk of immune rejection and secondary surgery in clinical applications. This strategy offers a facile route to regenerate large-scale robust skin, which shows great potential in abdominal wall defect repair.

3.
Cell Biochem Funct ; 2020 Jun 11.
Artigo em Inglês | MEDLINE | ID: mdl-32526811

RESUMO

Gastric cancer (GC) remains one of prevalent causes of cancer-related deaths worldwide. Long noncoding RNA is related to various cancers. Our study was conducted to explore the biological effects of LINC00240 on the tumorigenesis of GC and uncover its possible mechanisms. LINC00240 expression was determined in GC cell lines and samples through quantitative Real-time Polymerase Chain Reaction (qRT-PCR). The functional effects of LINC00240 were validated using in vitro and in vivo assays. Targets were assessed by AGO2-dependent RNA immunoprecipitation assay and dual-luciferase report assays. Our findings suggested higher LINC00240 expression in GC tissues and cells. Through downregulating LINC00240, cell proliferation, invasion and migration were retarded in vitro, and tumorigenesis of GC cells was notably suppressed in vivo. Further research showed that LINC00240 was a cytoplasmic lncRNA that shared miRNA response elements of microRNA (miR)-124-3p with DNMT3B, thus forming a LINC00240/miR-124-3p/DNMT3B axis explaining the functions of LINC00240. In a word, our study reveals that LINC00240 promotes GC tumorigenesis via a LINC00240/miR-124-3p/DNMT3B axis as an oncogene. These findings objectivise that LINC00240 may be a potential diagnostic biomarker for GC. SIGNIFICANCE OF THE STUDY: Gastric cancer (GC) is the fifth most common cancer and the third leading cause of cancer-related death across the world. Then we analysed lncRNA microarray of GC and selected LINC00240 as the study object. Therefore, the potential molecular mechanism as well as physiological function of LINC00240 in GC was discussed. Then we reveal that LINC00240 acts as an oncogene in GC progression via the miR-99a-5p/IGF1R axis. This study is the first to demonstrate the roles of LINC00240 in GC.

4.
Oxid Med Cell Longev ; 2020: 1404915, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32587656

RESUMO

Aseptic loosening caused by wear particles is one of the common complications after total hip arthroplasty. We investigated the effect of the recombinant protein ephB4-Fc (erythropoietin-producing human hepatocellular receptor 4) on wear particle-mediated inflammatory response. In vitro, ephrinB2 expression was analyzed using siRNA-NFATc1 (nuclear factor of activated T-cells 1) and siRNA-c-Fos. Additionally, we used Tartrate-resistant acid phosphatase (TRAP) staining, bone pit resorption, Enzyme-linked immunosorbent assay (ELISA), as well as ephrinB2 overexpression and knockdown experiments to verify the effect of ephB4-Fc on osteoclast differentiation and function. In vivo, a mouse skull model was constructed to test whether the ephB4-Fc inhibits osteolysis and inhibits inflammation by micro-CT, H&E staining, immunohistochemistry, and immunofluorescence. The gene expression of ephrinB2 was regulated by c-Fos/NFATc1. Titanium wear particles activated this signaling pathway to the promoted expression of the ephrinB2 gene. However, ephrinB2 protein can be activated by osteoblast membrane receptor ephB4 to inhibit osteoclast differentiation. In in vivo experiments, we found that ephB4 could regulate Ti particle-mediated imbalance of OPG/RANKL, and the most important finding was that ephB4 relieved the release of proinflammatory factors. The ephB4-Fc inhibits wear particle-mediated osteolysis and inflammatory response through the ephrinB2/EphB4 bidirectional signaling pathway, and ephrinB2 ligand is expected to become a new clinical drug therapeutic target.

5.
Artigo em Inglês | MEDLINE | ID: mdl-32410267

RESUMO

Carbon quantum dots (CDs) have inspired vast interests due to their prominent photoluminescence (PL) performances and promising applications in optoelectronic, biomedical and sensing fields. Notably, the development of effective approaches for large-scale CDs production may greatly promote the further advancement of their practical applications. In this review, the newly emerging methods for the large-scale production of CDs are summarized, such as microwave, ultrasonic, plasma, magnetic hyperthermia and microfluidic technology. Then, we introduce the available strategies for constructing CDs/polymer composites with intriguing solid-state PL. Particularly, the multiple roles of CDs are emphatically presented, including fillers, monomers and initiators. Moreover, the typical applications of CDs/polymer composites in light-emitting diodes, fluorescent printing and biomedicine are outlined. Finally, we discuss their current problems and speculate on their future development.

6.
ACS Nano ; 2020 Apr 21.
Artigo em Inglês | MEDLINE | ID: mdl-32298072

RESUMO

The astrophysical phenomenon of mimetic helical magnetic field (hB)-assisted self-assembly is herein introduced to build helical superstructures that display chiroptical properties. As a building block, magnetoplasmonic (MagPlas) Ag@Fe3O4 core-shell nanoparticles are used to guide plasmonic Ag nanoparticles onto a helical magnetic flux. The chirality of the assembled helical structures and tailored circular dichroism are successfully tuned in real time, and the handedness of the assembled structures is dynamically switched by the hB at the millisecond level, which is at least 6000-fold faster than other template-assisted methods. The peak position of circular dichroism can be reconfigured by altering the plasmonic resonance or coupling by controlling the size of the Ag core and magnetic flux density. The hB-induced chirality modulation represents a method to control the polarization state of light at the nexus of plasmonics, magnetic self-assembly, colloidal science, liquid crystals, and chirality. It presents active and dynamic chiral assemblies of magnetoplasmonic nanomaterials, enabling further practical applications in optical devices.

7.
Eur J Pharmacol ; 879: 173134, 2020 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-32339511

RESUMO

Cervical cancer is one of the leading causes of cancer death in women worldwide. While molecular mechanisms of initiation and cervical carcinogenesis are not well studied. Our data showed that the expression of Methyltransferase-like 3 (METTL3) was upregulated in cervical tumor tissues as compared with normal tissues. Its expression was associated with poor prognosis of cervical cancer. Knockdown of METTL3 can suppress the proliferation of cervical cancer cells. The expression of METTL3 was significantly correlated with the expression of RAB2B, one member of RAS oncogene family. Over expression of RAB2B can significantly attenuate sh-METTL3-suppressed cell proliferation. Mechanistically, METTL3 can increase the mRNA stability of RAB2B via an IGF2BP3-dependent manner. Collectively, METTL3 can trigger growth of cervical cancer cells via upregulation of RAB2B. It indicated that METTL3 might be a potential target for cervical cancer therapy.

8.
J Colloid Interface Sci ; 569: 1-11, 2020 Jun 01.
Artigo em Inglês | MEDLINE | ID: mdl-32092600

RESUMO

Whitlockite (WH, Ca18Mg2(HPO4)2(PO4)12) is the second most abundant bone mineral and has attracted attention as one of the novel bone regenerative materials. It has proven to enhance growth and promote osteogenesis of stem cells. However, investigating the mechanism of formation of pure phase WH nanocrystals remains a challenge. In this study, we introduced an interesting synthesis approach of WH nanocrystals using a tri-solvent system for the solid-liquid-solution (SLS) process. The ratio of precursor and reaction solvent composition was optimized to generate WH nanocrystals with tunable size, morphology (nanoplates, nanospheres), and surface properties (hydrophobic, hydrophilic), which is impossible to achieve using the traditional precipitation method. Molecular dynamics (MD) simulations revealed that the growth direction of nanoplates is highly related to the surfactant and its binding affinity. Finite element method (FEM) simulations elucidated that the ratio of ethanol/water plays an important role in defining the crystallinity and morphology of WH. In this study, we demonstrated that the cell proliferation of human bone marrow-derived mesenchymal stem cells (hBMSCs) is enhanced upon treatment with WH. The results of quantitative real-time polymerase chain reaction (qPCR) revealed that WH can positively accelerate the osteogenic differentiation in hBMSCs. The as-synthesized WH has a great potential in the future to be used in osteogenic tissue engineering. This study opens a new horizon for the synthesis and application of WH.


Assuntos
Materiais Biocompatíveis/química , Fosfatos de Cálcio/química , Células-Tronco Mesenquimais/metabolismo , Nanopartículas/química , Tecidos Suporte/química , Regeneração Óssea , Calcificação Fisiológica , Proliferação de Células , Células Cultivadas , Etanol/química , Análise de Elementos Finitos , Humanos , Interações Hidrofóbicas e Hidrofílicas , Simulação de Dinâmica Molecular , Osteogênese , Solventes/química , Propriedades de Superfície , Engenharia Tecidual , Água
9.
Nanoscale ; 12(9): 5494-5500, 2020 Mar 05.
Artigo em Inglês | MEDLINE | ID: mdl-32090221

RESUMO

Recently, widespread attention has been paid to red emissive carbon dots (CDs) which have desirable optical properties, low toxicity, and biocompatibility. Despite great efforts, the facile preparation of red dual-emissive CDs useful for ratiometric detection and bioimaging remains challenging. Here, we report a facile synthesis of red dual-emissive CDs and their potential for ratiometric fluorescence sensing and cellular imaging. Derived from the hydrothermal treatment of dicyandiamide and o-phenylenediamine in dilute sulfuric acid, the CDs are surface-tailored with nitrogen-, oxygen-, and sulfur-containing functional groups. The as-prepared CDs show various good features, including good water solubility, biocompatibility, excitation-independent dual-emission with two photoluminescence (PL) peaks centered at 630 and 680 nm, and an absolute quantum yield (QY) of 30.2% in water. The CDs exhibit a selective, sensitive, rapid, and stable ratiometric fluorescence response toward methyl blue, giving a linear relationship in the range of 0.5-300 µM with a correlation coefficient (R2) of 0.997. We also study ratiometric fluorescence sensing for the accurate detection of pH. Moreover, the CDs possess good cellular imaging ability, indicating their promising applicability for biomedical applications. These results pave a way toward the fabrication of red dual-emissive carbon-based nanomaterials useful for both ratiometric sensing and bioimaging.


Assuntos
Carbono/química , Pontos Quânticos/química , Corantes Fluorescentes/química , Células HeLa , Humanos , Concentração de Íons de Hidrogênio , Microscopia Confocal , Nitrogênio/química , Oxigênio/química , Pontos Quânticos/metabolismo , Enxofre/química
10.
Colloids Surf B Biointerfaces ; 189: 110839, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32036333

RESUMO

From senescence and frailty that may result from various biological, mechanical, nutritional, and metabolic processes, the human body has its own antioxidant defense enzymes to remove by-products of oxygen metabolism, and if unregulated, can cause several types of cell damage. Herein, an antioxidant, artificial nanoscale enzyme, called nanozyme (NZs), is introduced that is composed of Au nanoparticles (NPs) synthesized with a mixture of two representative phytochemicals, namely, gallic acid (GA) and isoflavone (IF), referred to as GI-Au NZs. Their unique antioxidant and anti-aging effects are monitored using Cell Counting Kit-8 and senescence-associated ß-galactosidase assays on neonatal human dermal fibroblasts (nHDFs). Furthermore, alterations in epidermal thickness and SOD activity are measured under ultraviolet light to investigate the effects of the topical application of NZs on the histological structure and antioxidant activity in hairless mice skin. Then, hepatotoxicity and nephrotoxicity in the hairless mice are monitored. It is concluded that the NZs can effectively prevent serial passage-induced senescence in nHDFs, as well as oxidative stress in mice skin, suggesting a range of strategies to further develop novel therapeutics for acute frailty.

11.
Environ Sci Pollut Res Int ; 27(5): 4840-4848, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31845258

RESUMO

Although triclosan (TCS) use is widespread in China, little is known about levels of exposure to TCS in pregnant women and its potential predictors, sources, and seasonal variability. We assessed urinary TCS levels of 466 pregnant women in a Chinese cohort. The estimated daily intake (EDI) and hazard quotient (HQ) were calculated. Potential predictors and sources were collected through a questionnaire and the seasonal variability was recorded based on the time of sampling. The geometric mean of urinary TCS concentration was 0.81 µg/g. The 95th EDI was 0.15 µg/kg BW per day and the corresponding HQ was 3.23 × 10-3. Women with a household monthly salary between RMB (¥) 1000 and 3000 and between RMB (¥) 3000 and 5000 had 0.52 µg/g (95% CI 0.08, 0.75) and 0.58 µg/g (95% CI 0.17, 0.79) lower urinary TCS levels than those with a household monthly salary of < RMB (¥) 1000, respectively. Urine samples collected in winter had lower TCS levels (geometric mean 0.72 µg/g) than in spring, summer, and autumn (geometric mean 0.82, 0.84, and 0.86 µg/g), although they were non-significant (P = 0.648). No association was found between drinking water and food consumed during pregnancy and TCS levels. The study population was ubiquitously exposed to a relatively low and safe dose of TCS. Women with lower household income tended to be exposed to higher levels of TCS.


Assuntos
Triclosan , China , Estudos de Coortes , Feminino , Humanos , Gravidez , Estações do Ano , Triclosan/análise , Triclosan/química
12.
Adv Sci (Weinh) ; 6(22): 1901694, 2019 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-31763152

RESUMO

All-inorganic halide perovskite nanocrystals (PNCs) have drawn increasing attention owing to their splendid optical properties. However, such nanomaterials suffer from intrinsic instability, greatly limiting their practical application. Meanwhile, environmental regulation has restricted the emissions of volatile organic compounds (VOCs), initiating a search for alternative approaches to PNC synthesis and film forming. Herein, fiber-spinning chemistry (FSC) is proposed for easy-to-perform synthesis of highly stable PNC fibrous films. The FSC process utilizes spinning fibers as reactors, reducing the generation of VOCs. This method enables the fabrication of CsPbX3 (X = Cl, Br, I) PNCs/poly(methyl methacrylate)/thermoplastic polyurethanes fibrous films at room temperature in one step, exhibiting tunable emission between 450 and 660 nm. Significantly, the in situ generation of PNCs in hydrophobic core-shell nanofibers results in highly improved fluorescence stability. PNCs/polymer fibrous films keep constant in photoluminescence (PL) after storage at atmosphere for 90 d and retain 82% PL after water immersion for 120 h (vs fluorescence quenching in 10 d in air or 5 h in water for pristine PNCs). The PNCs/polymer fibrous films endowed with superior optical stability and great flexibility show promising potentials in flexible optoelectronic applications. This work paves a facile way toward high-performance nanoparticles/polymer fibrous films.

13.
J Vis Exp ; (149)2019 07 17.
Artigo em Inglês | MEDLINE | ID: mdl-31380834

RESUMO

Protein-nucleic acid interactions play important roles in biological processes such as transcription, recombination, and RNA metabolism. Experimental methods to study protein-nucleic acid interactions require the use of fluorescent tags, radioactive isotopes, or other labels to detect and analyze specific target molecules. Biotin, a non-radioactive nucleic acid label, is commonly used in electrophoretic mobility shift assays (EMSA) but has not been regularly employed to monitor protein activity during nucleic acid processes. This protocol illustrates the utility of biotin labeling during in vitro enzymatic reactions, demonstrating that this label works well with a range of different biochemical assays. Specifically, in alignment with previous findings using radioisotope 32P-labeled substrates, it is confirmed via biotin-labeled EMSA that MEIOB (a protein specifically involved in the meiotic recombination) is a DNA-binding protein, that MOV10 (an RNA helicase) resolves biotin-labeled RNA duplex structures, and that MEIOB cleaves biotin-labeled single-stranded DNA. This study demonstrates that biotin is capable of substituting 32P in various nucleic acid-related biochemical assays in vitro.

14.
Angew Chem Int Ed Engl ; 58(38): 13556-13564, 2019 Sep 16.
Artigo em Inglês | MEDLINE | ID: mdl-31364237

RESUMO

Photonic crystals (PCs) have been widely applied in optical, energy, and biological fields owing to their periodic crystal structure. However, the major challenges are easy cracking and poor structural color, seriously hindering their practical applications. Now, hydrophobic poly(tert-butyl acrylate) (P(t-BA)) PCs have been developed with relatively lower glass transition temperature (Tg ), large crack-free area, excellent hydrophobic properties, and brilliant structure color. This method based on hydrophobic groups (tertiary butyl groups) provides a reference for designing new kinds of PCs via the monomers with relatively lower Tg . Moreover, the P(t-BA) PCs film were applied as the photoluminescence (PL) enhanced film to enhance the PL intensity of CdSe@ZnS QDs by 10-fold in a liquid-crystal display (LCD) device. The new-type hydrophobic force assembled PCs may open an innovative avenue toward new-generation energy-saving devices.

15.
Environ Int ; 129: 573-582, 2019 08.
Artigo em Inglês | MEDLINE | ID: mdl-31174145

RESUMO

BACKGROUND: Per- and polyfluoroalkyl substances (PFASs) are widely used in China, but little is known about the association between prenatal PFASs exposure and fetal reproductive development as well as its potential mechanism. OBJECTIVE: We investigated the effects of cord blood PFASs on fetal reproductive hormones and its potential mechanism in relation to steroidogenic enzymes. METHODS: Ten selected PFASs (n = 351) including PFOS, PFOA, PFBS, PFDA, PFDoA, PFHpA, PFHxS, PFNA, PFOSA, and PFUA, and two reproductive hormones estradiol (E2) (n = 351) and testosterone (T) (n = 349) were measured in 351 cord blood serum samples from a Chinese birth cohort between 2010 and 2013. Three steroidogenic enzymes including P450arom (n = 125), 3ß-HSD1 (n = 123), and 17ß-HSD1 (n = 116) were measured in 125 placental tissue samples. Linear regression tested the associations between cord blood PFASs and reproductive hormones in cord blood. Mediation analysis assessed the role of placental steroidogenic enzymes between cord blood PFASs and reproductive hormones. RESULTS: The positive associations between PFOA, PFHxS and E2 levels, PFOS, PFUA, PFNA and T levels, and PFOS, PFUA and T/E2 ratio were significant. PFUA, PFNA, PFDA, PFHxS, and ∑PFASs were associated with higher P450arom levels. PFHxS was also associated with increased 3ß-HSD1 and 17ß-HSD1 levels. These associations were more pronounced in females than males when stratified by gender. Furthermore, 17ß-HSD1 demonstrated mediating effects in the positive association between cord blood PFHxS and E2 levels in females. CONCLUSION: Our findings suggested the potential impacts of cord blood PFASs on fetal reproductive hormones, in which steroidogenic enzymes may play important roles. These associations were more pronounced in females than males.


Assuntos
Sistema Enzimático do Citocromo P-450/sangue , Poluentes Ambientais/sangue , Estradiol/sangue , Sangue Fetal/química , Fluorcarbonetos/sangue , Testosterona/sangue , China , Feminino , Humanos , Masculino , Placenta , Gravidez
16.
J Vis Exp ; (147)2019 05 10.
Artigo em Inglês | MEDLINE | ID: mdl-31132071

RESUMO

Spermatogenesis defines a highly ordered process of male germ cell differentiation in mammals. In testis, transcription and translation are uncoupled, underlining the importance of post-transcriptional regulation of gene expression orchestrated by RBPs. To elucidate mechanistic roles of an RBP, crosslinking immunoprecipitation (CLIP) methodology can be used to capture its endogenous direct RNA targets and define the actual interaction sites. The enhanced CLIP (eCLIP) is a newly-developed method that offers several advantages over the conventional CLIPs. However, the use of eCLIP has so far been limited to cell lines, calling for expanded applications. Here, we employed eCLIP to study MOV10 and MOV10L1, two known RNA-binding helicases, in mouse testis. As expected, we find that MOV10 predominantly binds to 3' untranslated regions (UTRs) of mRNA and MOV10L1 selectively binds to Piwi-interacting RNA (piRNA) precursor transcripts. Our eCLIP method allows fast determination of major RNA species bound by various RBPs via small-scale sequencing of subclones and thus availability of qualified libraries, as a warrant for proceeding with deep sequencing. This study establishes an applicable basis for eCLIP in mammalian testis.


Assuntos
Reagentes para Ligações Cruzadas/química , Imunoprecipitação/métodos , Proteínas de Ligação a RNA/metabolismo , RNA/metabolismo , Testículo/metabolismo , Animais , Masculino , Camundongos Endogâmicos C57BL , Raios Ultravioleta
17.
Environ Sci Pollut Res Int ; 26(19): 19403-19410, 2019 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-31073831

RESUMO

Although BPA use is widespread and often detectable in humans, little is known about its exposure levels and potential exposure predictors in pregnant women in China. We investigated the BPA exposure levels in pregnant women and its health implications and potential exposure predictors. Urinary BPA levels were measured for 506 pregnant women in northern China. Hazard quotients (HQs) based on estimated daily intakes (EDIs) were conducted. Sociodemographic characteristics and food consumption during pregnancy were collected and seasons of sample collection were recorded. The detection rate of urinary BPA was 86.6% and the median concentrations were 0.48 µg/L (1.05 µg/g creatinine). The EDI (median = 0.008 µg/kg bw/day) was much lower than the recommended tolerable daily doses and the HQ (median = 0.002) much lower than 1. The urine collected in summer had significantly higher BPA levels than that collected in other seasons (ß = 0.225; 95% CI - 0.008, 0.458; p = 0.03). Women "always consuming shellfish" had significantly higher BPA levels than those "seldom consuming shellfish" (ß = 0.341; 95% CI 0.022, 0.66; p = 0.04). The study found a wide exposure to BPA among pregnant women in this region, which might be associated with seasonal variation and shellfish consumption. Although the HQs suggested no obvious risk, further attention to the comprehensive exposure and potential determinants should be paid in view of its endocrine-disrupting potential.


Assuntos
Compostos Benzidrílicos/urina , Exposição Dietética/efeitos adversos , Disruptores Endócrinos/urina , Exposição Materna/efeitos adversos , Fenóis/urina , Adulto , China , Estudos de Coortes , Creatinina/análise , Exposição Dietética/análise , Feminino , Humanos , Valor Preditivo dos Testes , Gravidez , Medição de Risco , Alimentos Marinhos , Estações do Ano , Frutos do Mar , Fatores Socioeconômicos , Inquéritos e Questionários , Adulto Jovem
18.
BMC Biol ; 17(1): 39, 2019 05 14.
Artigo em Inglês | MEDLINE | ID: mdl-31088452

RESUMO

BACKGROUND: RNA regulation by RNA-binding proteins (RBPs) involve extremely complicated mechanisms. MOV10 and MOV10L1 are two homologous RNA helicases implicated in distinct intracellular pathways. MOV10L1 participates specifically in Piwi-interacting RNA (piRNA) biogenesis and protects mouse male fertility. In contrast, the functional complexity of MOV10 remains incompletely understood, and its role in the mammalian germline is unknown. Here, we report a study of the biological and molecular functions of the RNA helicase MOV10 in mammalian male germ cells. RESULTS: MOV10 is a nucleocytoplasmic protein mainly expressed in spermatogonia. Knockdown and transplantation experiments show that MOV10 deficiency has a negative effect on spermatogonial progenitor cells (SPCs), limiting proliferation and in vivo repopulation capacity. This effect is concurrent with a global disturbance of RNA homeostasis and downregulation of factors critical for SPC proliferation and/or self-renewal. Unexpectedly, microRNA (miRNA) biogenesis is impaired due partially to decrease of miRNA primary transcript levels and/or retention of miRNA via splicing control. Genome-wide analysis of RNA targetome reveals that MOV10 binds preferentially to mRNAs with long 3'-UTR and also interacts with various non-coding RNA species including those in the nucleus. Intriguingly, nuclear MOV10 associates with an array of splicing factors, particularly with SRSF1, and its intronic binding sites tend to reside in proximity to splice sites. CONCLUSIONS: These data expand the landscape of MOV10 function and highlight a previously unidentified role initiated from the nucleus, suggesting that MOV10 is a versatile RBP involved in a broader RNA regulatory network.


Assuntos
Células-Tronco Germinativas Adultas/metabolismo , RNA Helicases/genética , Espermatozoides/metabolismo , Animais , Perfilação da Expressão Gênica , Masculino , Camundongos , RNA Helicases/metabolismo
19.
Int J Hyg Environ Health ; 222(5): 896-902, 2019 06.
Artigo em Inglês | MEDLINE | ID: mdl-30981646

RESUMO

BACKGROUND: Several epidemiological studies suggest that prenatal exposure to BPA may interfere with the neurodevelopment of pre-school and school-age children. However, a limited number of studies are available for effects during children at a younger age, especially in China. METHODS: Based on Laizhou Wan Birth Cohort (LWBC), BPA concentrations were measured in urine among 506 pregnant women during their hospital admission for delivery and neurodevelopment of their children was assessed using the Gesell Development Schedules at 12 months (n = 368) and 24 months (n = 296). Linear regression and generalized linear models were used to analyze the association between prenatal BPA exposure and the children's developmental quotient scores (DQs). RESULTS: The median of maternal BPA concentration was 0.48 µg/L or 1.05 µg/g creatinine. Maternal BPA concentrations were adversely associated with children DQs at 12 months of age, with a 10-fold increase in prenatal BPA concentrations correlated to 1.43-point decrease in DQs in the adaptive domain (ß = -1.43; 95% CI: -2.30 to -0.56, p = 0.001). When stratified by gender, prenatal BPA concentrations were adversely associated with the adaptive domain DQs among boys (p-trend = 0.012) and girls (p-trend = 0.028) and the social domain DQs (p-trend = 0.019) only among girls. At 24 months of age, the significant adverse association was only found in the language domain among girls (ß = -1.69; 95% CI: -3.23 to -0.15, p = 0.032). CONCLUSION: Based on a Chinese population, we found potential impacts of prenatal BPA exposure on childhood neurodevelopment at 12 and 24 months of age, especially among girls.


Assuntos
Compostos Benzidrílicos/toxicidade , Desenvolvimento Infantil/efeitos dos fármacos , Fenóis/toxicidade , Efeitos Tardios da Exposição Pré-Natal , Compostos Benzidrílicos/urina , Pré-Escolar , China , Feminino , Humanos , Lactente , Fenóis/urina , Gravidez
20.
Soft Matter ; 15(12): 2517-2525, 2019 Mar 20.
Artigo em Inglês | MEDLINE | ID: mdl-30672942

RESUMO

Gels with multiple stimuli-responsive actuating behaviour have shown great potential in many applications. Nevertheless, facile approaches to rapidly preparing gel actuators are still highly needed, and obtaining gels possessing both actuating and auto-healing capabilities remains a challenge. Herein, we report the rapid preparation of gel actuators with a self-healing ability. Dual-component gels, composed of poly(BA-co-VI-co-AM) (G-1) and poly(BA-co-AA-co-AM/ß-CD) (G-2) (BA = butyl acrylate, VI = N-vinyl imidazole, AM = acrylamide, AA = acrylic acid, ß-CD = ß-cyclodextrin), are prepared within 10 minutes (min) via biphase frontal polymerization (FP). Both G-1 and G-2 gels show excellent intrinsic self-healing properties based on hydrogen bonds, with healing efficiencies of 91% and 97%, respectively; self-healing between G-1 and G-2 also occurs due to hydrogen bonding and host-guest interactions. Moreover, dual-component gels, in terms of G-1 and G-2 bilayer gel flowers and strips, heterogeneous healed bilayer gel strips, and microfluidic-directed bilayer gel microsphere ensembles, all show actuating behaviour in acidic, alkaline and organic solutions, with actuation degrees up to 96% in 5 min. The actuation mechanism is also proposed. This work might provide new insights into fast synthesis of self-healing dual-component gels towards application in the actuator field.

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