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1.
Am J Cancer Res ; 10(4): 1271-1273, 2020.
Artigo em Inglês | MEDLINE | ID: mdl-32368401

RESUMO

[This corrects the article on p. 1047 in vol. 5, PMID: 26045985.].

2.
Bioresour Technol ; 303: 122905, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32032938

RESUMO

A strain with efficient biofilm-formation and aerobic denitrification capabilities was isolated and identified as Pseudomonas mendocina IHB602. In pure culture, strain IHB602 removed almost all NO3--N, NO2--N, and NH4+-N (initial concentrations 50 mg/L) within 24 h. The strain produced large amounts of extracellular polymeric substances (maximum 430.33 mg/g cell dry weight) rich in protein but containing almost no humic acid. This, and strong autoaggregation (maximum 47.09%) and hydrophobicity (maximum 85.07%), imparted strain IHB602 with biofilm forming traits. A sequencing batch biofilm reactor bioaugmented with strain IHB602 (SBBR1) had more rapid biofilm-formation than the control without strain IHB602 inoculation (SBBR2). During the stabilization period, the effluent removal ratios for NH4+-N (95%), NO3--N (91%) and TN (88%) in SBBR1 were significantly higher than those in SBBR2 (NH4+-N: 91%, NO3--N: 88%, TN: 82%). Microbial community structure analysis revealed that strain IHB602 successfully proliferated and contributed to nitrogen removal as well as biofilm formation.


Assuntos
Nitrogênio , Águas Residuárias , Biofilmes , Reatores Biológicos , Desnitrificação , Eliminação de Resíduos Líquidos
3.
BMC Pharmacol Toxicol ; 20(1): 61, 2019 10 28.
Artigo em Inglês | MEDLINE | ID: mdl-31661009

RESUMO

BACKGROUND: In recent years, targeted therapy has received widespread attention. Among these therapies, anti-angiogenic targeted drugs have become one of the hotspots of research. Apatinib is a novel oral small molecule anti-angiogenic agent that has been clinically tested in a variety of solid tumours. The aim of this study was to investigate the efficacy of apatinib in patients with advanced malignant tumours and failure of standard therapy. METHODS: We collected 41 patients with advanced malignant tumours in our department; all tumours were pathologically confirmed as malignant. All patients received apatinib after failure of standard therapy: 500 mg/dose, one dose/d, orally 30 min after a meal, until progressive disease or intolerable adverse reactions occurred. When there was a second- or third-degree adverse reaction associated with apatinib during treatment, apatinib treatment could be suspended or reduced to 250 mg/dose. Clinical efficacy and progression-free survival were assessed according to RECIST1.1, and adverse reactions were observed. RESULTS: Efficacy assessment was available for 31 patients with a median progression-free survival time of 2.66 months; the objective response rate and disease control rates were 16.1 and 64.5%, respectively. The disease control rates of the patients with lower Eastern Cooperative Oncology Group scores (1-2 points) and with fewer metastatic sites (< 3 sites) were higher than those of the patients with higher scores (3 points) and with more metastatic sites (≥3 sites), respectively (all P < 0.05). The most common adverse reactions were hypertension, neutropenia and hand-foot syndrome. CONCLUSION: For patients with advanced malignant tumours with failure of standard therapy, administration of apatinib can still result in good efficacy. The efficacy of apatinib is better in patients with a higher performance status and lower degree of tumour progression.

4.
Biochem Biophys Res Commun ; 519(4): 783-789, 2019 Nov 19.
Artigo em Inglês | MEDLINE | ID: mdl-31551150

RESUMO

Nuclear migration in Arabidopsis root hairs is bidirectional and relies on actin filaments. However, how actin filaments regulate the bidirectional movement of nuclei remains unclear. Here, we discovered that nuclei migrate forward and backward according to the developmental stage of the hair cells. In addition, the migration direction of nuclei was not constant but reversed occasionally, accompanied by nuclear shape changes. Confocal microscopic analysis revealed that perinuclear actin bundles were closely related to the migration and shape of hair cell nuclei. Pharmacological studies showed that SMIFH2, an inhibitor of the actin nucleator-formin, inhibited nuclear backward migration probably by impairing the perinuclear actin filaments. These data indicate that nuclear migration in hair cells is likely motivated by the competition of mechanical forces acting on the nucleus. Furthermore, the perinuclear actin filaments are closely related to the migration direction of hair cell nuclei.

5.
Reprod Biol Endocrinol ; 17(1): 71, 2019 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-31472681

RESUMO

BACKGROUND: Palmitic acid (PA) is a common saturated fatty acid that induces apoptosis in various types of cells, including testicular Leydig cells. There is evidence suggesting that PA is increased in patients with obesity and that PA-induced cell apoptosis may play an important role in obesity-related male infertility. Curcumin, a natural polyphenol, has been reported to exert cytoprotective effects in various cell types. However, the cytoprotective effect of curcumin against PA-induced apoptosis in Leydig cells remains unknown. Therefore, the current study was performed to investigate the protective effects of curcumin in response to PA-induced toxicity and apoptosis in murine Leydig tumor cell line 1 (MLTC-1) cells and explore the mechanism underlying its anti-apoptotic action. METHODS: MLTC-1 cells were cultured in Roswell Park Institute-1640 medium and divided into five groups. First four groups were treated with 50-400 µM PA, 400 µM PA + 5-40 µM curcumin, 400 µM PA + 500 nM 4-phenylbutyric acid (4-PBA, an endoplasmic reticulum (ER) stress inhibitor), and 500 nM thapsigargin (TG, an ER stress inducer) + 20 µM curcumin, respectively, followed by incubation for 24 h. Effects of PA and/or curcumin on viability, apoptosis, and ER stress in MLTC-1 cells were then determined by cell proliferation assay, flow cytometry, and western blot analysis. The fifth group of MLTC-1 cells was exposed to 400 µM of PA and 5 IU/mL of human chorionic gonadotropin (hCG) for 24 h in the absence and presence of curcumin, followed by measurement of testosterone levels in cell-culture supernatants by enzyme-linked immunosorbent assay (ELISA). Rats fed a high-fat diet (HFD) were treated with or without curcumin for 4 weeks, and the testosterone levels were detected by ELISA. RESULTS: Exposure to 100-400 µM PA reduced cell viability, activated caspase 3, and enhanced the expression levels of the apoptosis-related protein BCL-2-associated X protein (BAX) and ER stress markers glucose-regulated protein 78 (GRP78) and CCAAT/enhancer binding protein homologous protein (CHOP) in MLTC-1 cells. Treating cells with 500 nM 4-PBA significantly attenuated PA-induced cytotoxicity through inhibition of ER stress. Curcumin (20 µM) significantly suppressed PA- or TG-induced decrease in cell viability, caspase 3 activity, and the expression levels of BAX, CHOP, and GRP78. In addition, treating MLTC-1 cells with 20 µM curcumin effectively restored testosterone levels, which were reduced in response to PA exposure. Similarly, curcumin treatment ameliorated the HFD-induced decrease in serum testosterone level in vivo. CONCLUSIONS: The present study suggests that PA induces apoptosis via ER stress and curcumin ameliorates PA-induced apoptosis by inhibiting ER stress in MLTC-1 cells. This study suggests the application of curcumin as a potential therapeutic agent for the treatment of obesity-related male infertility.


Assuntos
Apoptose/efeitos dos fármacos , Curcumina/farmacologia , Estresse do Retículo Endoplasmático/efeitos dos fármacos , Células Intersticiais do Testículo/efeitos dos fármacos , Ácido Palmítico/farmacologia , Animais , Anti-Inflamatórios não Esteroides/farmacologia , Proteínas Reguladoras de Apoptose/metabolismo , Linhagem Celular Tumoral , Sobrevivência Celular/efeitos dos fármacos , Células Intersticiais do Testículo/citologia , Células Intersticiais do Testículo/metabolismo , Masculino , Camundongos , Fenilbutiratos/farmacologia , Substâncias Protetoras/farmacologia , Ratos Sprague-Dawley , Testículo/citologia
6.
Bioresour Technol ; 293: 122083, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31487615

RESUMO

A novel aerobic denitrifying strain Methylobacterium gregans DC-1 was isolated and identified. Strain DC-1 removed 98.4% of nitrate-nitrogen (NO3--N) and 80.7% of total organic carbon with initial concentrations of 50 and 2400 mg/l, respectively. The N balance showed that most NO3--N was converted to N2 (62.18%) without nitrous oxide (N2O) emission. Response surface analysis showed that the optimal conditions for total N removal were carbon (C):N ratio of 18.7, temperature of 26.8 °C, pH of 6.5 and shaking speed of 180 rpm. In combination with the N balance and successful amplification of napA, nirK and nosZ genes, the metabolic pathway was as follows: NO3-NO2- → NO → N2O → N2. Strain DC-1 had strong auto-aggregation rate (maximum 38.7%), produced large amounts of extracellular polymeric substances (EPS; maximum of 781.4 mg/g cell dry weight) and had corresponding strong hydrophobicity (maximum 83.2%). Pearson correlation analysis showed that EPS content and hydrophobicity were significantly positively correlated with auto-aggregation.


Assuntos
Methylobacterium , Óxido Nitroso , Desnitrificação , Nitratos , Nitrogênio
7.
AMB Express ; 9(1): 129, 2019 Aug 19.
Artigo em Inglês | MEDLINE | ID: mdl-31428884

RESUMO

The responses of denitrifiers and denitrification ability to dissolved oxygen (DO) concent in different layers of surface lake sediments are still poorly understood. Here, the optimal denitrification condition was constructed based on response surface methodology (RSM) to analyze the denitrification characteristics of surface sediments. The aerobic zone (AEZ), hypoxic zone (HYZ), up-anoxic zone (ANZ-1) and sub-anoxic zone (ANZ-2) were partitioned based on the oxygen contents, and sediments were collected using a customized-designed sub-millimeter scale sampling device. Integrated real-time quantitative PCR, Illumina Miseq-based sequencing and denitrifying enzyme activities analysis revealed that denitrification characteristics varied among different DO layers. Among the four layers, the DNA abundance and RNA expression levels of norB, nirS and nosZ were the highest at the aerobic layer, hypoxic layer and up-axoic layer, respectively. The hypoxia and up-anaerobic layer were the active nitrogen removal layers, since these two layers displayed the highest DNA abundance, RNA expression level and enzyme activities of denitrification functional genes. The abundance of major denitrifying bacteria showed significant differences among layers, with Azoarcus, Pseudogulbenkiania and Rhizobium identified as the main nirS, nirK and nosZ-based denitrifiers. Pearson's correlation revealed that the response of denitrifiers to environmental factors differed greatly among DO layers. Furthermore, napA showed higher DNA abundance and RNA expression level in the aerobic and hypoxic layers than anaerobic layers, indicating that aerobic denitrifiers might play important roles at these layers.

8.
Int Immunopharmacol ; 75: 105822, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31437793

RESUMO

Metformin, the most widely used medicine for type 2 diabetes, displays anti-inflammatory functions via activating AMP-activated protein kinase (AMPK). Circulating autoantibodies and disequilibrium of helper T cells and regulatory T cells are pathological hallmarks of myasthenia gravis (MG). Rectify the imbalance of different T cell populations has become an important therapeutic strategy to treat MG. In this study, we assessed the effect of metformin on the development of autoimmunity using an experimental autoimmune myasthenia gravis (EAMG) rat model. We first provided evidence that oral administration of metformin attenuated the onset of EAMG. This effect was accompanied by a substantial decrease of circulating auto-antibody levels with no effect on blood glucose level. While metformin treatment in vitro showed little effect on inducible Treg, metformin strongly inhibited Th17 cell differentiation through the increase of reactive oxygen species and AMPK. Furthermore, an attenuation of antigen-induced IgG2b antibody production by two different doses of metformin was also observed in the AChR-specific recall response. In conclusion, the above results indicate that metformin may have therapeutic value for the clinical treatment of MG.


Assuntos
Anti-Inflamatórios/uso terapêutico , Hipoglicemiantes/uso terapêutico , Metformina/uso terapêutico , Miastenia Gravis Autoimune Experimental/tratamento farmacológico , Proteínas Quinases Ativadas por AMP/imunologia , Animais , Anti-Inflamatórios/farmacologia , Anticorpos/sangue , Linfócitos B/efeitos dos fármacos , Linfócitos B/imunologia , Glicemia/efeitos dos fármacos , Modelos Animais de Doenças , Feminino , Hipoglicemiantes/farmacologia , Metformina/farmacologia , Ratos Endogâmicos Lew , Espécies Reativas de Oxigênio/imunologia , Receptores Colinérgicos/imunologia , Linfócitos T Reguladores/efeitos dos fármacos , Linfócitos T Reguladores/imunologia , Células Th17/efeitos dos fármacos , Células Th17/imunologia
9.
J Biol Chem ; 294(36): 13336-13343, 2019 Sep 06.
Artigo em Inglês | MEDLINE | ID: mdl-31320475

RESUMO

Dynamic control of thioredoxin (Trx) oxidoreductase activity is essential for balancing the need of cells to rapidly respond to oxidative/nitrosative stress and to temporally regulate thiol-based redox signaling. We have previously shown that cytokine stimulation of the respiratory epithelium induces a precipitous decline in cell S-nitrosothiol, which depends upon enhanced Trx activity and proteasome-mediated degradation of Txnip (thioredoxin-interacting protein). We now show that tumor necrosis factor-α-induced Txnip degradation in A549 respiratory epithelial cells is regulated by the extracellular signal-regulated protein kinase (ERK) mitogen-activated protein kinase pathway and that ERK inhibition augments both intracellular reactive oxygen species and S-nitrosothiol. ERK-dependent Txnip ubiquitination and proteasome degradation depended upon phosphorylation of a PXTP motif threonine (Thr349) located within the C-terminal α-arrestin domain and proximal to a previously characterized E3 ubiquitin ligase-binding site. Collectively, these findings demonstrate the ERK mitogen-activated protein kinase pathway to be integrally involved in regulating Trx oxidoreductase activity and that the regulation of Txnip lifetime via ERK-dependent phosphorylation is an important mediator of this effect.

10.
J Environ Sci (China) ; 84: 184-196, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31284910

RESUMO

Lake Caohai has experienced extensive Microcystis blooms in recent years, and to improve its water quality, the local government carried out a series of water control measures. To better understand the dynamics of both pelagic and benthic Microcystis and their characteristics in Lake Caohai, we conducted a 1-year investigation from December 2015 to December 2016 to gain a seasonal outlook on the distribution and dynamics of cell abundance, colony size and intracellular microcystins (MCs) of Microcystis. The results indicated that the Microcystis bloom occupied primarily the northeastern region and then moved gradually from lakeshore to lake center. The perennial southwesterly winds and the water inflow from northeast to southwest in Lake Caohai determined the spatiotemporal distribution of pelagic Microcystis. Benthic Microcystis was mainly distributed in the northeastern region in summer, occupied the lake center in autumn and then occupied the southeastern region in winter, determined by the sedimentation of pelagic Microcystis and the death of benthic Microcystis. Small colonies (20-60 µm) overwintered more easily in both water column and sediment. The concentrations of intracellular toxin of benthic Microcystis were observed to be significantly higher than those of pelagic Microcystis. This might be because Microcystis synthesized large amount of MCs to acclimate to an unfavorable benthic environment. This knowledge on the dynamics of Microcystis expands our understanding of mechanisms underpinning the formation of Microcystis blooms.


Assuntos
Lagos/microbiologia , Microcystis/metabolismo , Biomassa , China , Estações do Ano
11.
J Mol Model ; 25(2): 30, 2019 Jan 07.
Artigo em Inglês | MEDLINE | ID: mdl-30617436

RESUMO

The reactions between arsenic and nitrogen oxides (N2O, NO2, and NO) were investigated using density functional theory. The geometries of the reactants, intermediates, transition states, and products in each reaction were optimized. Frequency analysis was applied to verify those geometries, and the authenticity of each transition state was checked using intrinsic reaction coordinate analysis (IRC). The single point energy of each stationary point was calculated at the B2PLYP level, and kinetic analysis was conducted to explore each reaction mechanism in more detail. Results showed that the energy barriers to the reactions of As with N2O, NO2, and NO were 78.45, 2.58, and 155.85 kJ mol-1, respectively. For each reaction, the rate increased as the temperature was increased from 298 to 1800 K. However, temperature had only a tiny impact on the reaction of As with NO2 due to the low energy barrier involved, and the reaction rate was consistently high (>1012 cm3 mol-1 s-1), which indicates that this reaction occurs readily. On the other hand, the rate of the reaction between As and N2O or NO increased rapidly between 298 and 900 K, and then increased more gradually upon further increasing the temperature.

12.
Int J Biol Macromol ; 125: 116-123, 2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-30508543

RESUMO

As a novel class of noncoding RNAs (ncRNAs), circular RNAs (circRNAs) have been verified to be potential biomarkers and therapeutic targets for human malignant tumors. However, the thorough understanding of circRNAs in the progression of esophageal squamous cell carcinoma (ESCC) still needs to be improved. This study focused on exploring the function and mechanism of circVRK1 in ESCC. At first, we examined the expression level of circVRK1 in ESCC tissues and cell lines with qRT-PCR. We found that circVRK1 was downregulated in ESCC tissues and cell lines. Kaplan-Meier method was used to analyze the correlation between circVRK1 expression and the overall survival of ESCC patients. Functionally, overexpression of circVRK1 suppressed the cell proliferation, migration and epithelial-mesenchymal transition (EMT) and reversed the radioresistance. Therefore, we identified the tumor suppressive role of circVRK1 in ESCC progression. Mechanistically, circVRK1 positively regulated PTEN by acting as a molecular sponge of miR-624-3p. Moreover, circVRK1 decreased the activity of PI3K/AKT signaling pathway by upregulating PTEN. Rescue assays were carried out to confirm the function of circVRK1-miR-624-3p-PTEN axis in ESCC progression. Our findings showed that circVRK1 suppressed ESCC progression by regulating miR-624-3p/PTEN axis and PI3K/AKT signaling pathway, suggesting the potential therapeutic value of circVRK1 for ESCC.


Assuntos
Carcinoma de Células Escamosas do Esôfago/genética , Carcinoma de Células Escamosas do Esôfago/metabolismo , Peptídeos e Proteínas de Sinalização Intracelular/sangue , MicroRNAs/genética , PTEN Fosfo-Hidrolase/metabolismo , Fosfatidilinositol 3-Quinases/metabolismo , Proteínas Serina-Treonina Quinases/sangue , Proteínas Proto-Oncogênicas c-akt/metabolismo , Linhagem Celular Tumoral , Movimento Celular , Proliferação de Células , Transição Epitelial-Mesenquimal/genética , Carcinoma de Células Escamosas do Esôfago/patologia , Carcinoma de Células Escamosas do Esôfago/radioterapia , Perfilação da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Genes Reporter , Humanos , Tolerância a Radiação/genética , Transdução de Sinais
13.
J Invest Surg ; 32(5): 433-441, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-29381439

RESUMO

Purpose: To determine the efficacy and safety of corticosteroid injection for trigger finger by performing a meta-analysis of all relevant studies. Methods: PubMed, EMBASE, and Cochrane Library databases were searched for randomized controlled trials (RCTs) comparing corticosteroid injection with other treatments for trigger finger. Pooled summary estimates for outcomes, including success rate, relapse rate, visual analogue score (VAS) and complications, were calculated as standardized mean difference (SMD) or relative risk (RR) either on a fixed- or random-effect model via Stata 12.0 software. Results: Ten literatures involving 806 patients (387 in corticosteroid injection group and 419 in control group) were included. Pooled analysis showed there were no differences in the success rate, VAS and complications between patients undergoing corticosteroid injection and others. However, the relapse rate was significantly higher in patients treated with corticosteroid injection than that of other treatments (RR = 19.53, 95% CI = 6.23-61.19). Subgroup analysis indicated the efficacy of corticosteroid injection was superior to other non-surgical treatments (success rate: RR = 1.54, 95% CI = 1.01-2.35), but inferior to surgery (success rate: RR = 0.55, 95% CI = 0.48-0.63; relapse rate: RR = 21.15, 95% CI = 6.06-73.85; VAS: SMD = 3.49, 95% CI = 2.84-4.14). Conclusions: Corticosteroid injection may be an effective strategy for management of trigger finger, although surgery may be needed for some patients due to recurrence.


Assuntos
Glucocorticoides/administração & dosagem , Dor Musculoesquelética/tratamento farmacológico , Manejo da Dor/métodos , Dedo em Gatilho/terapia , Tratamento Conservador/métodos , Humanos , Injeções Intralesionais , Dor Musculoesquelética/diagnóstico , Dor Musculoesquelética/etiologia , Procedimentos Ortopédicos , Medição da Dor , Ensaios Clínicos Controlados Aleatórios como Assunto , Recidiva , Resultado do Tratamento , Dedo em Gatilho/complicações
14.
Int J Mol Sci ; 19(12)2018 Nov 22.
Artigo em Inglês | MEDLINE | ID: mdl-30469514

RESUMO

The angiosperm pollen tube delivers two sperm cells into the embryo sac through a unique growth strategy, named tip growth, to accomplish fertilization. A great deal of experiments have demonstrated that actin bundles play a pivotal role in pollen tube tip growth. There are two distinct actin bundle populations in pollen tubes: the long, rather thick actin bundles in the shank and the short, highly dynamic bundles near the apex. With the development of imaging techniques over the last decade, great breakthroughs have been made in understanding the function of actin bundles in pollen tubes, especially short subapical actin bundles. Here, we tried to draw an overall picture of the architecture, functions and underlying regulation mechanism of actin bundles in plant pollen tubes.


Assuntos
Citoesqueleto de Actina/metabolismo , Tubo Polínico/metabolismo , Actinas/metabolismo , Magnoliopsida/metabolismo , Proteínas dos Microfilamentos/metabolismo , Proteínas de Plantas/metabolismo
15.
Toxicol Appl Pharmacol ; 352: 68-76, 2018 08 01.
Artigo em Inglês | MEDLINE | ID: mdl-29800642

RESUMO

Perfluorooctanoic acid (PFOA), a wide spread environmental pollutant, was associated with developmental cardiotoxicity in chicken embryo, while the underlying molecular mechanism had not been fully elucidated. In the current study, 2 mg/kg (egg weight) PFOA and/or 100 mg/kg (egg weight) l-carnitine were exposed to embryonic day zero (ED0) chicken embryo via air cell injection, and then bone morphogenic protein 2 (BMP2) silencing lentivirus or BMP2 recombinant protein were introduced into ED2 embryo. Electrocardiography and histological methods were utilized to assess the cardiac function and morphology in hatchling chickens, respectively. Consistent with previous results, 2 mg/kg PFOA exposure at ED0 significantly elevated heart rate and thinned right ventricular wall in hatchling chickens, while l-carnitine co-treatment reverted such changes. BMP2 silencing induced very similar changes in hatchling chicken hearts as PFOA exposure, while co-exposure of recombinant BMP2 protein alleviated PFOA-induced changes. l-carnitine exposure alleviated the BMP2-silencing induced changes as well. Western blotting revealed that PFOA exposure enhanced BMP2 expression and suppressed pSMAD1 expression in ED15 chicken embryo hearts, while both changes were reverted by l-carnitine co-exposure. Furthermore, silencing of BMP2 significantly increased the expression level of PPAR alpha in ED15 chicken embryo hearts, while silencing of PPAR alpha did not have significant impact on BMP2 expression. In conclusion, BMP2/pSMAD1 signaling participates in the PFOA-induced developmental cardiotoxicity in chicken embryo, which is likely located upstream of PPAR alpha for this particular endpoint. Protection of BMP2 signaling might contribute to l-carnitine mediated protection against PFOA-induced developmental cardiotoxicity.


Assuntos
Proteína Morfogenética Óssea 2/farmacologia , Caprilatos/toxicidade , Carnitina/farmacologia , Poluentes Ambientais/toxicidade , Fluorcarbonetos/toxicidade , Cardiopatias/prevenção & controle , Coração/efeitos dos fármacos , Animais , Proteína Morfogenética Óssea 2/genética , Proteína Morfogenética Óssea 2/metabolismo , Cardiotoxicidade , Embrião de Galinha , Citoproteção , Regulação da Expressão Gênica no Desenvolvimento , Inativação Gênica , Coração/embriologia , Coração/fisiopatologia , Cardiopatias/induzido quimicamente , Cardiopatias/embriologia , Cardiopatias/fisiopatologia , Frequência Cardíaca/efeitos dos fármacos , PPAR alfa/genética , PPAR alfa/metabolismo , Fosforilação , Proteínas Recombinantes/farmacologia , Transdução de Sinais/efeitos dos fármacos , Proteína Smad1/genética , Proteína Smad1/metabolismo , Função Ventricular Direita/efeitos dos fármacos , Remodelação Ventricular/efeitos dos fármacos
16.
J Biosci Bioeng ; 126(3): 339-345, 2018 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-29680369

RESUMO

AD-1, an aerobic denitrifier, was isolated from activated sludge and identified as Pseudomonas stutzeri. AD-1 completely removed NO3- or NO2- and removed 99.5% of NH4+ during individual culturing in a broth medium with an initial nitrogen concentration of approximately 50 mg L-1. Results showed that larger amounts of nitrogen were removed through assimilation by the bacteria. And when NH4+ was used as the sole nitrogen source in the culture medium, neither NO2- nor NO3- was detected, thus indicating that AD-1 may not be a heterotrophic nitrifier. Only trace amount of N2O was detected during the denitrification process. Single factor experiments indicated that the optimal culture conditions for AD-1 were: a carbon-nitrogen ratio (C/N) of 15, a temperature of 25°C and sodium succinate or glucose as a carbon source. In conclusion, due to the ability of AD-1 to utilize nitrogen of different forms with high efficiencies for its growth while producing only trace emissions of N2O, the bacterium had outstanding potential to use in the bioremediation of high-nitrogen-containing wastewaters. Meanwhile, it may also be a proper candidate for biotreatment of high concentration organic wastewater.


Assuntos
Desnitrificação , Nitrogênio/isolamento & purificação , Nitrogênio/metabolismo , Pseudomonas stutzeri/metabolismo , Purificação da Água/métodos , Aerobiose , Biodegradação Ambiental , Carbono/química , Carbono/metabolismo , Processos Heterotróficos , Humanos , Pseudomonas stutzeri/isolamento & purificação , Esgotos/química , Esgotos/microbiologia , Temperatura , Águas Residuárias/química
17.
Mol Med Rep ; 17(5): 6828-6834, 2018 May.
Artigo em Inglês | MEDLINE | ID: mdl-29512751

RESUMO

Haishengsu (HSS) is an active natural extract isolated from Tegillarca granosa, which has previously been demonstrated to inhibit the proliferation of several types of cancer cells in vitro. Our previous study indicated that HSS may induce apoptosis to suppress growth of human hepatocellular carcinoma BEL­7402 cells by activating Fas pathway. The present study demonstrated that HSS treatment induces the in vitro apoptosis of BEL­7402 cells via the mitochondrial­mediated apoptotic pathway detected by DNA fragmentation assay, caspase activity assay and transmission electron microscopy assay, and inhibits tumor xenograft growth in vivo. Alterations in apoptotic regulatory proteins were detected, including decreased expression of B­cell lymphoma2 (Bcl­2), upregulation of Bcl­2­associated X protein and mitochondrial cytochrome c release, and downstream activation of apoptotic signaling. Furthermore, apoptotic induction was caspase­dependent, as indicated by cleavage of the caspase substrate, poly (ADP­ribose) polymerase. Oral administration of 62.5­250 mg/kg HSS markedly educed the growth of hepatocellular carcinoma tumor xenografts in nude mice. In addition, immunohistochemical staining for caspase­3 protein and transmission electron microscopy further indicated the induction of apoptosis in these tumor tissues. Taken together, the present study demonstrated that HSS may effectively induce apoptosis to suppress the growth of BEL­7402 cells in vitro and in vivo, and therefore may hold promise for further development as a novel cancer therapy.


Assuntos
Apoptose/efeitos dos fármacos , Bivalves/química , Carcinoma Hepatocelular , Misturas Complexas/farmacologia , Neoplasias Hepáticas , Mitocôndrias/metabolismo , Animais , Carcinoma Hepatocelular/tratamento farmacológico , Carcinoma Hepatocelular/metabolismo , Carcinoma Hepatocelular/patologia , Caspase 3/metabolismo , Linhagem Celular Tumoral , Misturas Complexas/química , Humanos , Neoplasias Hepáticas/tratamento farmacológico , Neoplasias Hepáticas/metabolismo , Neoplasias Hepáticas/patologia , Camundongos , Camundongos Endogâmicos BALB C , Camundongos Nus , Mitocôndrias/patologia , Proteínas Proto-Oncogênicas c-bcl-2/metabolismo , Ensaios Antitumorais Modelo de Xenoenxerto , Proteína X Associada a bcl-2/metabolismo
18.
Environ Sci Pollut Res Int ; 25(12): 11767-11774, 2018 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-29442311

RESUMO

The NO emission characteristics of Datong bituminous coal and Yangquan anthracite in O2/H2O/CO2 atmospheres were investigated by using a fixed-bed reactor system, and the emission characteristics were compared with the experimental results from O2/N2 and O2/CO2 atmospheres, especially at low O2 concentrations and high temperatures. The results showed that NO emissions of pulverized coal in O2/CO2 environments were less than those in the O2/N2 environments, regardless of the O2 concentration and the furnace temperature. Adding H2O decreased the possibility of reactions between the reductive groups (NH) and the oxygen radical during devolatilization, which led to a decrease in NO emissions at 1000 °C. However, as the furnace temperature increased, "additional" nitrogen precursors (HCN and NH3) generated by enhanced char-H2O gasification were quickly oxidized to generate a large amount of NO during char oxidation that exceeded the amount of NO reduced by NH during devolatilization. Thus, the NO emissions in O2/CO2/H2O atmosphere were higher than those in O2/CO2 atmosphere at a low O2 concentration. However, as the O2 concentration increased, the NO emissions in O2/CO2/H2O atmosphere became lower than those in O2/CO2 atmosphere because the effect of H2O gasification became weaker. The NO emissions of Yangquan anthracite (YQ) were higher than those of DT, but the changing trend of YQ was similar to that of DT.


Assuntos
Atmosfera , Carvão Mineral , Óxido Nítrico/química , Água/química , Temperatura Alta , Oxigênio/química
19.
J Int Med Res ; 46(1): 158-167, 2018 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-28758851

RESUMO

Objective To identify potential genes that may be involved in lipid metabolism in rats after treatment with aqueous extract of Arctium lappa L (burdock). Methods Rats were randomly divided into six groups: (i) control (standard diet); (ii) model group (high-fat diet only); (iii) high-fat diet and low-dose aqueous burdock root extract (2 g/kg); (iv) high-fat diet and moderate-dose aqueous burdock root extract (4 g/kg); (v) high-fat diet and high-dose aqueous burdock root extract (8 g/kg); and (vi) a positive control group exposed to a high-fat diet and simvastatin (10 mg/kg). Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was performed to find the potential candidate genes involved in the modulation of blood lipids by treatment with aqueous burdock root extract. Results Burdock root extract reduced body weight and cholesterol levels in rats. KEGG analysis revealed 113 genes that were involved in metabolic pathways. Of these, 27 potential genes associated with blood lipid metabolism were identified. Conclusions Aqueous extract of burdock root reduced body weight and cholesterol in rats, possibly by modulating the differential expression of genes.


Assuntos
Arctium/química , Dieta Hiperlipídica , Regulação da Expressão Gênica , Hipolipemiantes/farmacologia , Metabolismo dos Lipídeos/efeitos dos fármacos , Extratos Vegetais/farmacologia , Animais , Peso Corporal/efeitos dos fármacos , HDL-Colesterol/sangue , LDL-Colesterol/sangue , Enciclopédias como Assunto , Perfilação da Expressão Gênica , Ontologia Genética , Hipolipemiantes/química , Metabolismo dos Lipídeos/genética , Masculino , Anotação de Sequência Molecular , Extratos Vegetais/química , Raízes de Plantas/química , Ratos , Ratos Sprague-Dawley , Sinvastatina/farmacologia , Solventes/química , Triglicerídeos/sangue , Água/química
20.
Zhongguo Fei Ai Za Zhi ; 20(12): 847-851, 2017 Dec 20.
Artigo em Chinês | MEDLINE | ID: mdl-29277185

RESUMO

Non-small cell lung cancer is one of the most commom malignant tumor being harmful to people's life and health. Most of the patients have developed to the last stage which not suitable for surgical indications, so radiation and chemotherapy is the main treatment strategy. In recent years, with the theory of anti-angiogenesis therapy for malignant tumors, apatinib as a promising novel medicine to treat malignant tumors, represents synergistic antitumor effects in combination with radiotherapy. The underlying mechanisms may include make blood vessel normalization, alleviating inner hypoxia, and angiogenic factors regulation. Apatinib in combination with radiotherapy may become a new and effective treatment strategy of non-small cell lung cancer.


Assuntos
Carcinoma Pulmonar de Células não Pequenas/terapia , Quimiorradioterapia/métodos , Neoplasias Pulmonares/terapia , Piridinas/uso terapêutico , Animais , Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico , Carcinoma Pulmonar de Células não Pequenas/radioterapia , Humanos , Neoplasias Pulmonares/tratamento farmacológico , Neoplasias Pulmonares/radioterapia
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