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1.
Environ Pollut ; 259: 113943, 2020 Jan 09.
Artigo em Inglês | MEDLINE | ID: mdl-32023797

RESUMO

Heavy metal (HM) stress is a major hazard, which significantly affects plant growth and development. In order to confront HM stress, plants directly or indirectly regulate the levels of endogenous nitric oxide (NO), a redox-related signaling molecule involved in wide range of plant growth and development as well as in response to HM stress. In addition, there is now compelling experimental evidence that NO usually mediates signaling processes through interactions with different biomolecules like phytohormones to regulate HM tolerance. Apart from phytohormones, NO partly operates through posttranslational modification of proteins, notably via S-nitrosylation in response to HM stress. Recently, the roles of S-nitrosylation as a regulator of plant responses to HM stress and S-nitrosylated candidates have also been established and detected. Here, we describe the roles of NO in confronting HM phytotoxicity in plants with a particular focus on the presentation and discussion of recent data obtained in this field, which involves in the function of various phytohormones and S-nitrosylation during plant responses to HM stress. Additionally, both importance and challenges of future work are outlined in order to further elucidate the specific mechanisms underlying the roles of NO in plant responses to HM stress.

2.
Nanotechnology ; 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32031997

RESUMO

Antimony (Sb) anode has attracted increasing attention since high theoretical capacity and suitable working potential. Nonetheless, its practical application is largely challenged by huge volume changes during the cyclic process, resulting in the unsatisfied long-term cycled stabilities at high current density. In this work, large-scale ultrafine Sb nanoparticles are functionally designed to encapsulate into 3D carbon microfiber framework via a scalable electrospinning approach followed by a thermal treatment process. This fabrication strategy effectively avoids the volume change of Sb anode and provides a fast conductive network to serve an efficient 3D e/Li+ transport pathway. Benefiting from this novel structure-design, ultrafine Sb nanoparticles@carbon microfiber framework (U-Sb-NPs@CMF) composite anode used as LIBs delivers a high reversible capacity of 622 mAh g-1 after 200 cycles at 0.5 A g-1, 507 mAh g-1 at 2 A g-1 after 2000 cycles and high capacity retention of 350 mAh g-1 even after 5000 long-term cycles. These outstanding charge/discharge performances suggest that the U-Sb-NPs@CMF composite is substitude one of promising candidate of anode materials for the application of LIBs.

3.
J Am Chem Soc ; 142(5): 2150-2154, 2020 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-31955571

RESUMO

The reaction between SO3 and water in the gas phase has always been of great interest, as it has important implications in atmospheric and environmental science. Compared to gas-phase water, however, heterogeneous hydration of SO3 on the surfaces of condensed phases of water/ice is relatively less explored. Here, we present a systematic study of the reactions between SO3 and three different phases of water, namely, water vapor, the surface of a liquid water droplet, and the {1 1 -2 0} plane of hexagonal ice (Ih). The computational results show that, contrary to the gas-phase water, the surface of a water droplet and the {1 1 -2 0} plane of Ih ice play distinctly different roles in the reaction, in which the HSO4-/H3O+ is formed within a few picoseconds. Moreover, the SO3 hydration exhibits multiple reaction pathways on the surface of a water droplet and the {1 1 -2 0} plane of Ih ice, including a newly observed chemical mechanism without the formation of water-loop structures. Considering temperature effects (winter vs summer), SO3/water vapor concentrations in the atmosphere, and the effective surface areas of water droplets in the atmosphere and ice on the ground, the reaction rates of SO3 with water in the gas phase, in aerosols, in clouds, and on snowpack are estimated and compared. Consistent with previous experimental studies, the loss rates of SO3 due to aerosols and clouds are less important compared to that due to water vapor. Surprisingly, the ice snowpack is shown to be an efficient sink for SO3 depletion, especially in the winter season.

4.
Plant Mol Biol ; 102(3): 271-285, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31838617

RESUMO

KEY MESSAGE: H2 prolonged the vase life and improved the vase quality of cut roses through repressing endogenous ethylene production and alleviating ethylene signal transduction during the entire senescing period. Recently, the application of hydrogen gas (H2) was shown to improve postharvest quality and longevity in perishable horticultural products, but the specific regulation mechanism remains obscure. Here, endogenous ethylene production and the expression of genes in ethylene biosynthesis and signalling pathway were investigated to explore the crosstalk between H2 and ethylene during the senescence of cut roses. Our results revealed that addition of exogenous ethylene by ethephon accelerated the senescence of cut roses, in which 100 mg L-1 ethephon displayed the most obvious senescent phenotype. While the applied different concentrations (1%, 10%, 50% and 100%) of hydrogen-rich water (HRW) conducted different affects in alleviating the senescence of cut roses, and 1% HRW displayed the best ornamental quality and the longest vase life by reducing ethylene production, supported by the decrease of 1-aminocyclopropene-1-carboxylate (ACC) accumulation, ACC synthase (ACS) and ACC oxidase (ACO) activities, and Rh-ACS3 and Rh-ACO1 expressions in ethylene biosynthesis. In addition, HRW increased the transcripts of ethylene receptor genes Rh-ETR1 at blooming period from day 4 to day 6 and suppressed Rh-ETR3 at senescence phase at day 8 after harvest. Furthermore, the relevant affection of HRW on Rh-ETR1 and Rh-ETR3 expressions still existed when the ethylene production was compromised by adequate addition of exogenous ethylene in HRW-treated cut rose petals, and HRW directly repressed the protein level of Rh-ETR3 in a transient expression assay. Overall, the results suggested that H2 is involved in neutralizing ethylene-mediated postharvest in cut flowers.

5.
J Pharm Pharmacol ; 72(3): 461-469, 2020 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-31858612

RESUMO

OBJECTIVE: To investigate the effect of GSK-137647A, the first non-carboxylic FFA4 agonist, on osteogenic and adipogenic differentiation of bone mesenchymal stem cells (BMSCs) of db/db mice. METHODS: Bone mesenchymal stem cells were extracted from 8-week-old db/db mice. Cell Counting Kit-8 was used to evaluate the toxicity of GSK-137647A on BMSCs, and the optimal concentration of GSK-137647A was selected to investigate the osteogenic and adipogenic differentiation of BMSCs, and relevant indicators of osteoblasts and adipocytes were detected. KEY FINDINGS: GSK-137647A had no significant toxicity on cell growth and proliferation. Moreover, GSK-137647A showed a significant increase in mineralization of BMSCs differentiated osteoblasts compared to the control group and elevated the alkaline phosphatase (ALP) activity in a time-dependent manner. Meanwhile, the treatment of GSK-137647A decreased the adipogenic differentiation of BMSCs. The expression levels of ALP, runt-related transcription factor 2, bone morphogenetic protein 4, osterix and ß-catenin were significantly increased in GSK-137647A-treated group, while the gene and protein levels of peroxisome proliferator-activated receptor γ and CCAAT/enhancer binding protein α were significantly reduced. CONCLUSIONS: All of these results demonstrated that GSK-137647A suppressed the adipogenic differentiation and promoted osteogenic differentiation of BMSCs, which is partly attributed to the increased expression of ß-catenin in wingless/integrated signalling pathway.

6.
Plant Physiol Biochem ; 147: 161-171, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31865162

RESUMO

Recently, nitric oxide (NO), a redox-related signaling molecule, is considered to be a key regulator in plant growth and development as well as response to abiotic stresses. Heavy metal (HM) stress is one of the most serious threats to affect crop growth and production. HM stress attributes to the production of reactive oxygen species (ROS), leading to oxidative stress in plants. Thus, to minimize the toxic effects of HM stress, plants directly or indirectly activate different ROS-scavenging mechanisms comprised antioxidative enzymes and non-enzymatic antioxidants. Understanding the roles of NO is essential to elucidate how NO activates the appropriate set of responses to HM stress. Moreover, the regulation of key genes or proteins is very important in response to stress stimuli. Therefore, here we focus on the recent knowledge concerning the alleviating effect of NO on HM stress, covering from HM iron accumulation to antioxidant system to related gene and protein expression.

7.
Carbohydr Polym ; 227: 115338, 2020 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-31590863

RESUMO

New sources of AIE materials with good water solubility, biocompatibility, degradability, and mass production are urgently needed. Here, we found that chitosan, a very abundant polysaccharide in nature, has fluorescence emission in both solid and solution states with AIE effect, and explored its multifunctional applications. Chitosan can emit a variety of colors from blue to red at different excitation wavelengths with excellent multicolor imaging capabilities at the cellular level. Utilizing the cationic and antibacterial properties of chitosan, the quantification of bacteria can be achieved through the AIE effect. Concurrently, it can be used as fluorescent probes for multi-channel bacterial imaging via lighting-up bacteria. Furthermore, the chitosan solution exhibits a sensitive quenching response to Fe3+, which can be used as a biosensor for detecting the concentration of Fe3+. These interesting results indicate that chitosan will have broad application prospects as a new class of AIE material.


Assuntos
Materiais Biocompatíveis , Quitosana , Fluorescência , Escherichia coli , Humanos , Ferro , Células MCF-7 , Microscopia Confocal , Espectrometria de Fluorescência
8.
Phys Chem Chem Phys ; 22(1): 258-264, 2019 Dec 18.
Artigo em Inglês | MEDLINE | ID: mdl-31808477

RESUMO

Using all-atomic molecular dynamics (MD) simulations, we show that the structure of interfacial water (IW) induced by substrates characterizes the ability of a substrate to nucleate ice. We probe the shape and structure of ice nuclei and the corresponding supercooling temperatures to measure the ability of IW with various hydrogen polarities for ice nucleation, and find that the hydrogen polarization of IW even with the ice-like oxygen lattice increases the contact angle of the ice nucleus on IW, thus lifting the free energy barrier of heterogeneous ice nucleation. The results show that not only the oxygen lattice order but the hydrogen disorder of IW on substrates are required to effectively facilitate the freezing of top water.

9.
Mar Drugs ; 17(12)2019 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-31847202

RESUMO

In recent years, the wide application of exopolysaccharides (EPSs) in food, cosmetics, medicine, and other fields has drawn tremendous attention. In this study, an EPS produced by Pseudoalteromonas agarivorans Hao 2018 was isolated and purified, and its fermentation conditions were optimized. Its structure and biological functions were also studied. The purity and molecular weight of EPS were determined by high performance liquid chromatography (HPLC), and the EPS exhibited a number average of 2.26 × 105 and a weight average of 2.84 × 105. EPS has good adsorption for Cu2+ and Pb2+. The adsorption rates can reach up to 69.79% and 82.46%, respectively. The hygroscopic property of EPS was higher than that of chitosan, but slightly lower than that of sodium hyaluronate. However, the water-retaining activity of EPS was similar to that of chitosan and sodium hyaluronate. EPS has strong ability to scavenge free radicals, including OH radical and O2- radical. Further, its activity on O2- radicals has similarities with that of vitamin C. EPS has broad application prospects in many fields, such as cosmetics, environmental protection.

10.
Dalton Trans ; 2019 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-31774092

RESUMO

2D materials have strong intermolecular van der Waals forces, and 2D superlattice heterostructures have exhibited many dramatic photo-electrochemical properties for energy conversion and storage. Herein, based on the excellent properties of reduced graphene and superlattice structures, we constructed a 2D flexible superlattice polyoxometalate/rGO heterojunction with enhanced electron-hole separation via interfacial self-assembly engineering to further fabricate DSSCs based on the heterojunction-modified photoanode, which exhibited good electron transport properties. Selecting two kinds of Dawson POMs (P2W15V3, P2W18 and the corresponding heteropoly blue) as the research object, the polyoxometalate superlattice structure was obtained by the self-assembly strategy, and characterized by IR, UV-Vis, XRD, EDX and XPS. The TEM and AFM results indicated that the monolayer POM superlattice structure and superlattice polyoxometalate/rGO heterojunction were successfully obtained. The superlattice P2W18(HPB)/rGO heterojunction was introduced into the DSSCs photoanode, and electrochemical tests indicated that the superlattice polyoxometalate/rGO heterojunction improved the electron-hole separation rate, inhibited the electron recombination, and improved the photoelectric conversion efficiency to 8.09%. The 2D superlattice heterojunction remarkably improved the electrochemical performances of the energy storage and conversion systems.

11.
Luminescence ; 2019 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-31667940

RESUMO

The emission wavelength of organic-inorganic hybrid perovskite quantum dots (QDs) can be tuned by controlling reaction time relevant to the halide exchange. It is because halide exchange with different time would lead to different molar ratio of halides in perovskite QDs such as Cl and Br. Here, to research the ligand's effect on the halide exchange, this work synthesized 3-mercaptopropionic acid (MPA)-capped CH3 NH3 PbBrx Cl3-x QDs. It was found that SH- of MPA appeared to inhibit the halide exchange during the reation. Moreover, although the MPA-capped CH3 NH3 PbBrx Cl3-x QDs did not contain the chiral centre, they exhibit the optical rotation. This may provide a method for chirality manipulation of perovskite.

12.
Mol Ther Methods Clin Dev ; 15: 257-263, 2019 Dec 13.
Artigo em Inglês | MEDLINE | ID: mdl-31720304

RESUMO

Adeno-associated virus (AAV) vectors are clinically proven gene delivery vehicles that are attracting an increasing amount of attention. Non-genome-containing empty AAV capsids are by-products during AAV production that have been reported to potentially impact AAV product safety and efficacy. Therefore, the presence and amount of empty AAV capsids need to be characterized during process development. Multiple methods have been reported to characterize empty AAV capsid levels, including transmission electron microscopy (TEM), analytical ultracentrifugation (AUC), charge detection mass spectrometry (CDMS), UV spectrophotometry, and measuring capsid and genome copies by ELISA and qPCR. However, these methods may lack adequate accuracy and precision or be challenging to transfer to a quality control (QC) lab due to the difficulty of implementation. In this study, we used AAV serotype 6.2 (AAV6.2) as an example to show the development of a QC-friendly anion exchange chromatography (AEX) assay for the determination of empty and full capsid percentages. The reported assay requires several microliters of material with a minimum titer of 5 × 1011 vg/mL, and it can detect the presence of as low as 2.9% empty capsids in AAV6.2 samples. Additionally, the method is easy to deploy, can be automated, and has been successfully implemented to support testing of various in-process and release samples.

13.
J Phys Chem Lett ; 10(23): 7318-7323, 2019 Dec 05.
Artigo em Inglês | MEDLINE | ID: mdl-31713426

RESUMO

Single-atom catalysts have recently been subject to considerable attention within applied catalysis. However, complications in the preparation of well-defined single-atom model systems have hampered efforts to determine the reaction mechanisms underpinning the reported activity. By means of an atomic layer deposition method utilizing the steric hindrance of the ligands, isolated Fe1O3 motifs were grown on a single-crystal Cu2O(100) surface at densities up to 0.21 sites per surface unit cell. Ambient pressure X-ray photoelectron spectroscopy shows a strong metal-support interaction with Fe in a chemical state close to 3+. Results from scanning tunneling microscopy and density functional calculations demonstrate that isolated Fe1O3 is exclusively formed and occupies a single site per surface unit cell, coordinating to two oxygen atoms from the Cu2O lattice and another through abstraction from O2. The isolated Fe1O3 motif is active for CO oxidation at 473 K. The growth method holds promise for extension to other catalytic systems.

14.
Retina ; 2019 Nov 12.
Artigo em Inglês | MEDLINE | ID: mdl-31725525

RESUMO

PURPOSE: To evaluate the efficacy and primary safety of treating rhegmatogenous retinal detachment (RRD) using foldable capsular vitreous body scleral buckling. METHODS: Five patients with simple RRD were treated with foldable capsular vitreous body scleral buckling. B-ultrasound and fundus photography examining of retina reattachment were used to evaluate the postsurgery efficacy. The safety of the treatment was evaluated based on the occurrence of infection, eye pain, diplopia, elevated intraocular pressure, and other serious postoperative complications after surgery. The observation time after the operation was at least 12 weeks. RESULTS: The simple RRD of all five patients was successfully reattached before being evaluating by B-ultrasound and fundus photography after surgery. Visual acuity was enhanced in two patients who were macularly affected. One patient had temporary diplopia and eye movement limitation after surgery. No other complications were recorded. CONCLUSION: This pilot study determined that foldable capsular vitreous body scleral buckling can be efficacious and safe for simple RRD. The results indicate that this surgery may be a novel alternative to the current extraocular procedures for simple RRD.

15.
Cell Mol Neurobiol ; 2019 Nov 15.
Artigo em Inglês | MEDLINE | ID: mdl-31728694

RESUMO

The mammalian target of rapamycin (mTOR) was reported to regulate cell autophagy and outcomes of several neurological diseases. Mitochondria, which serve as critical organelles in neurons. are also involved in the pathology of neurological diseases. However, the role of mTOR in mitochondrial morphology has not been clarified especially in subarachnoid hemorrhage (SAH). In this study, we established SAH models both in vivo and in vitro. Rapamycin and 3-methyl adenine (3-MA) were then administered to alter mTOR activity. Post-SAH assessment included SAH grading, neurological evaluation, blood-brain barrier (BBB) permeability, brain water content, mitochondrial membrane potential (MMP), mitochondrial morphology, ATP content, cell viability, cytotoxicity, and expression of proteins related to apoptosis and mitochondrial fission. The results showed that (1) neurological deficits, BBB permeability, and brain edema were increased after SAH and that cell viability was exacerbated in brain tissue. (2) Excessive mitochondrial fission was evident based on changes in mitochondrial morphology, while MMP and ATP content were decreased in neurons after SAH. (3) Administration of rapamycin improved the excessive mitochondrial fission and restored mitochondrial function, which subsequently reduced apoptosis. (4) 3-MA showed an adverse effect on mitochondria and aggravated excessive mitochondrial fission and dysfunction in SAH. Neurological deficits and neuronal viability were also exacerbated following the administration of 3-MA. Therefore, our study suggests that mTOR inhibition has neuroprotective effects against neuronal injury after SAH via alleviating excessive mitochondrial fission.

16.
ACS Appl Mater Interfaces ; 11(51): 47671-47679, 2019 Dec 26.
Artigo em Inglês | MEDLINE | ID: mdl-31633335

RESUMO

Lead halide perovskite nanocrystals (NCs) are promising optical materials in many fields. However, their poor moisture stability, significant toxicity, and difficulty to be further functionalized greatly hinder their applications in bioimaging. Here, a universal strategy is demonstrated by simply encapsulating CsPbX3 (X = Cl, Br, I) NCs into phospholipids to achieve CsPbX3-phospholipid micelles (CsPbX3@phospholipid) as probes for multiplex encoding cellular imaging or tumor-targeted imaging. The layer of phospholipids endows CsPbX3 NCs with superior water-resistant characteristics, the ability to be further biofunctionalized, and greatly improved biocompatibility. The CsPbX3@phospholipid micelles exhibited strong luminescence with narrow fwhm in water for more than four months. Specifically, even after being modified with folic acid, the bright fluorescence of the micelles was well retained, which were employed for the targeting of Hela cells. Finally, the greatly reduced toxicity of the CsPbX3@phospholipid micelles was verified using HeLa cells and zebrafish as in vitro and in vivo models, respectively.

17.
Microsyst Nanoeng ; 5: 46, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31636935

RESUMO

Miniaturized enzymatic biofuel cells (EBFCs) with high cell performance are promising candidates for powering next-generation implantable medical devices. Here, we report a closed-loop theoretical and experimental study on a micro EBFC system based on three-dimensional (3D) carbon micropillar arrays coated with reduced graphene oxide (rGO), carbon nanotubes (CNTs), and a biocatalyst composite. The fabrication process of this system combines the top-down carbon microelectromechanical systems (C-MEMS) technique to fabricate the 3D micropillar array platform and bottom-up electrophoretic deposition (EPD) to deposit the reduced rGO/CNTs/enzyme onto the electrode surface. The Michaelis-Menten constant KM of 2.1 mM for glucose oxidase (GOx) on the rGO/CNTs/GOx bioanode was obtained, which is close to the KM for free GOx. Theoretical modelling of the rGO/CNT-based EBFC system via finite element analysis was conducted to predict the cell performance and efficiency. The experimental results from the developed rGO/CNT-based EBFC showed a maximum power density of 196.04 µW cm-2 at 0.61 V, which is approximately twice the maximum power density obtained from the rGO-based EBFC. The experimental power density is noted to be 71.1% of the theoretical value.

18.
Ying Yong Sheng Tai Xue Bao ; 30(10): 3491-3500, 2019 Oct.
Artigo em Chinês | MEDLINE | ID: mdl-31621236

RESUMO

The dynamic simulation of cucumber growth and development in sunlight greenhouse can provide technical support for the intelligent management of cucumber production. According to the cucumber response characteristics to light and temperature, the cucumber development module based on the algorithm of clock model was established by using data from four-stage experiment with 'Jinyou 35' as experiment variety in two years. Based on the relationship between the leaf growth and key meteorological factors (temperature and radiation), leaf area index (LAI) module was established with the accumulated product of thermal effectiveness and photosynthetically active radiation (TEP) as independent variables. The simulation module of cucumber dry matter production was established by taking into consideration the double integral of LAI and daily length in photosynthesis per unit leaf area as well as the respiratory expenditure of different organs. Combined with water content of organs, fresh weight simulation module of cucumber organs was constructed. The whole cucumber development and growth simulation model in greenhouse was built based on each sub-module. The model parameters were calibrated and determined. The results showed that root mean square error (RMSE) of simulated values and observed values of four deve-lopment stages (from transplanting date to stretch tendril, to initial flowering, to early harvested and to uprooting), was 3.9-10.5 d. The normalized root mean square error (nRMSE) was 6.5%-28.6%. The coincidence index (D) was 0.79-0.97. The relationship between LAI and TEP was the regression of 'S' type curve. The RMSE of simulated and observed LAI values was 0.19. The nRMSE was 17.2%. The D value was 0.90. The RMSE of dry weight of root, stem, leaf, flower and fruit of the simulated values and observed values were 0.39-8.94 g·m-2. The nRMSE were 10.9%-17.7%. The D values were all above 0.98. The growth and development model of cucumber could accurately simulate the key development period of cucumber, leaf area and the dry and fresh weight of various organs and quantify the growth and development of cucumber in sunlight greenhouse.


Assuntos
Cucumis sativus , Crescimento e Desenvolvimento , Fotossíntese , Folhas de Planta , Luz Solar
19.
MAbs ; 11(8): 1452-1463, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31570042

RESUMO

Protein primary structure is a potential critical quality attribute for biotherapeutics. Identifying and characterizing any sequence variants present is essential for product development. A sequence variant ~11 kDa larger than the expected IgG mass was observed by size-exclusion chromatography and two-dimensional liquid chromatography coupled with online mass spectrometry. Further characterization indicated that the 11 kDa was added to the heavy chain (HC) Fc domain. Despite the relatively large mass addition, only one unknown peptide was detected by peptide mapping. To decipher the sequence, the transcriptome of the manufacturing cell line was characterized by Illumina RNA-seq. Transcriptome reconstruction detected an aberrant fusion transcript, where the light chain (LC) constant domain sequence was fused to the 3' end of the HC transcript. Translation of this fusion transcript generated an extended peptide sequence at the HC C-terminus corresponding to the observed 11 kDa mass addition. Nanopore-based genome sequencing showed multiple copies of the plasmid had integrated in tandem with one copy missing the 5' end of the plasmid, deleting the LC variable domain. The fusion transcript was due to read-through of the HC terminator sequence into the adjacent partial LC gene and an unexpected splicing event between a cryptic splice-donor site at the 3' end of the HC and the splice acceptor site at the 5' end of the LC constant domain. Our study demonstrates that combining protein physicochemical characterization with genomic and transcriptomic analysis of the manufacturing cell line greatly improves the identification of sequence variants and understanding of the underlying molecular mechanisms.

20.
Medicine (Baltimore) ; 98(38): e17177, 2019 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-31567958

RESUMO

Lumbar spinal canal stenosis (LSCS) associated with degenerative scoliosis has being increasingly aware by the public and studied by many researchers. Degenerative changes leading to spinal stenosis can precede a spinal deformity which will develop into the de novo scoliosis. There are few studies focusing on the risk factors contributing to the degenerative lumbar scoliosis (DLS) in lumbar spinal stenosis patients.From September, 2017 to December, 2017, 181 patients who were diagnosed with LSCS in the outpatient department of our hospital were enrolled in this retrospective investigation. The patients were divided into 2 groups: DLS group (Cobb angle >10°) and LSCS group. Sex, age, smoking status (yes or no), occupation (heavy or light labor), body mass index (BMI), bone mineral density (BMD) and radiographic parameters including the lumbar lordosis (LL), pelvic incidence (PI), pelvic tilt (PT), sacral slope (SS), thoracic kyphosis (TK), coronal vertical axis, and sagittal vertical axis (SVA) are all evaluated as potential risk factors. Multivariate logistic regression analysis and receiver-operating characteristic curves were used to identify potential risk factors.Forty-five of 181 patients were diagnosed with DLS and involved in the DLS group. There were significant differences between the 2 groups in BMI (P < .001), LL (P = .0046), BMD (P < .001), SVA (P < .001), and TK (P = .047). BMD < -1.85 g/cm (adjusted odds ratio [AOR] 0.030, 95% confidence interval [CI] 0.008-0.107, P < .001), BMI >25.57 kg/m (AOR 1.270, 95% CI 1.040-1.551, P = .019), and SVA >3.98 cm (AOR 3.651, 95% CI 2.226-5.990, P < .001) had good accuracy to predict the formation of degenerative lumbar scoliosis based on degenerative lumbar spinal stenosis.Degenerative lumbar scoliosis has a high incidence in degenerative lumbar spinal stenosis. BMD <-1.85 g/cm, BMI >25.57 kg/m, and SVA >3.98 cm were the potential risk factors for the formation of degenerative lumbar scoliosis based on degenerative lumbar spinal stenosis.


Assuntos
Vértebras Lombares , Escoliose/complicações , Estenose Espinal/etiologia , Estudos Transversais , Feminino , Humanos , Vértebras Lombares/diagnóstico por imagem , Vértebras Lombares/patologia , Masculino , Pessoa de Meia-Idade , Radiografia , Fatores de Risco , Escoliose/diagnóstico por imagem , Escoliose/patologia , Estenose Espinal/diagnóstico por imagem , Estenose Espinal/patologia
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