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1.
Biotech Histochem ; : 1-8, 2020 May 04.
Artigo em Inglês | MEDLINE | ID: mdl-32362142

RESUMO

Local action of nicotine on oral mucosa contributes to the pathogenesis of precancerous and cancerous lesions. Nicotine participation in the mechanism of apoptosis in normal mucosa has not been established. Peroxiredoxin 1 (Prx1) is a cellular antioxidant that participates in regulating apoptosis. We investigated expression of Prx1 and proteins in apoptosis-related downstream signaling by mitogen-activated protein kinases (MAPKs) in nicotine-treated tongue tissues of wild-type and Prx1 knockout (Prx1±) mice; we also investigated these processes in mouse embryonic fibroblast (MEF) cells in vitro. Nicotine increased the expression of Prx1 mRNA in tongue tissues in vivo. The rate of apoptosis was similar among the nicotine-treated mice, nicotine-treated + Prx1± mice and untreated controls. The expression of p-JNK was greater in Prx1± mice compared to control mice. In MEF cells, nicotine increased the expression of Prx1 and inhibited apoptosis and expression of p-p38 and p-JNK. Prx1 knockdown animals exhibited increased apoptotic rate and expression of p-p38 and p-JNK in MEFs. Nicotine-regulated apoptosis might occur via a Prx1-dependent pathway.

2.
Artigo em Inglês | MEDLINE | ID: mdl-32379491

RESUMO

Background: FoxO3a is a well-studied tumor suppressor gene in forkhead transcriptional factor O (FoxO) subfamily and its downregulation is correlated with the occurrence of gastric cancer (GC). GC tissues had microRNA (miR)-372 upregulation, which has targeted relationship with 3'-untranslated region (3'-UTR) of FoxO3a gene. This study investigated if miR-372 plays a role in modulating FoxO3a expression, and affecting GC cell proliferation, apoptosis, and cisplatin (DDP) resistance. Materials and Methods: Dual luciferase reporter gene assay assessed the targeted regulation between miR-372 and FoxO3a. DDP-resistant cell lines MGC803/DDP and MKN28/DDP were compared for gene expression against parental cells. Cell proliferation was measured by Cell Counting Kit-8 (CCK-8) assay. Cultured cells were transfected with miR-372 mimic or miR-negative control (NC) to measure FoxO3a mRNA and protein expression. Cell apoptosis and proliferation were tested by flow cytometry and 5-Ethynyl-2'-deoxyuridine (EdU) staining, respectively. Results: miR-372 had a targeted relationship with FoxO3a mRNA. MGC803/DDP and MKN28/DDP cells had significantly elevated miR-372 level than parental cells, while Foxo3a mRNA or protein levels were significantly decreased. CCK-8 assay revealed significantly lower inhibitory activity on cell proliferation in drug-resistant cells. Compared with miR-NC group, miR-273 inhibitor transfected DDP-resistant cells had significantly increased Foxo3a expression, enhanced cell apoptosis, reduced proliferation, and drug resistance. Conclusions: miR-372 upregulation is associated with DPP resistance of GC cells. Downregulation of miR-372 can inhibit proliferation, facilitate apoptosis, and suppress DDP resistance of drug-resistant GC cells.

3.
Artif Cells Nanomed Biotechnol ; 48(1): 800-809, 2020 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-32432484

RESUMO

We used cell-free culture filtrate of Pseudomonas aeruginosa as a reducing mediator of AgNO3 to silvernanoparticles (AgNPs) and possibly used as a potential anticancer agent against thyroid cancer cells (TPC1). The bio-generation of AgNPs was firmly established by taking a UV spectrum at 380-500 nm wavelength. The Fourier transform spectrum analysis reveals the association of alcohol, phenol and aromatic functional groups with P. aeruginosa synthesized AgNPs (Ps-AgNPs). By observing under transmission electron microscopy (TEM), the size and structure of the Ps-AgNPs were characterized as the size was 30-70 nm and spherical in shape. The concentration-dependent cytotoxicity of Ps-AgNPs on TPC1 cells was observed and IC50 value was calculated. The alteration of oxidative and antioxidant biomarkers in Ps-AgNPs treated cells were observed. The induced apoptosis was determined by staining the Ps-AgNPs treated cells with DCFH-DA, Rh-123 dye, Acridine Orange (AO) and ethidium bromide (EtBr). Increased level of intracellular reactive oxygen species (ROS) generation and decreased level of mitochondrial membrane potential was observed in Ps-AgNPs treated TPC1 cells. Moreover, the apoptotic morphological changes were explored, which indicates increased apoptosis by inducing cell membrane damage in Ps-AgNPs treated cells. This biogenic approach will enable an effective and significant improvement in nano-oncotherapy.

4.
BMJ Open ; 10(5): e032242, 2020 May 18.
Artigo em Inglês | MEDLINE | ID: mdl-32430445

RESUMO

INTRODUCTION: The need for cataract surgery is on the rise due to our ageing population and high demands for greater visual functioning. Although the majority of patients want to participate in a shared decision-making process, no decision aid has been available to improve the quality of decision. The present study aims to determine whether a decision aid increases informed decision about cataract surgery. METHODS AND ANALYSIS: A parallel randomised controlled trial (772 participants) will be conducted. The decision aid will be implemented among patients with any age-related cataract in Yuexiu District, which is socioeconomically representative of a major metropolitan region in Southern China. Participants will be randomly assigned to receive either a patient decision aid or a traditional booklet, and they will complete three surveys: (1) baseline assessment before the intervention (time point (T)1), 2 weeks (T2) and 1 year (T3) after the intervention. The control group receives a traditional booklet with standard general information developed by the National Eye Institute to help patients understand cataract, whereas the intervention group receives a patient decision aid that includes not only the standard general information, but also the quantitative risk information on the possible outcomes of cataract surgery as well as value clarification exercise. The primary study outcome is the informed decision, the percentage of patients who have adequate knowledge and demonstrate consistency between attitudes and intentions. Secondary outcomes include perceived importance of cataract surgery benefits/harms, decision conflict and confidence, anticipated regret and booklet utilisation and acceptability at 2 weeks, and surgical rates and a cost-utility estimate of the decision aid at 1 year. ETHICS AND DISSEMINATION: Ethics approval was obtained from the Ethics Committee of Zhongshan Ophthalmic Center (reference number: 2019KYPJ090). Results will be published in peer-reviewed journals and presented at scientific meetings for academic audiences. TRIAL REGISTRATION NUMBER: NCT03992807.

5.
Biosci Rep ; 40(5)2020 May 29.
Artigo em Inglês | MEDLINE | ID: mdl-32342981

RESUMO

We aim to determine whether nebivolol has a better effect on endothelial dysfunction compared with other ß-blockers or other classes of antihypertensive drugs. Searches of the PubMed, Embase etc. were performed to analyze all the randomized controlled trials using nebivolol to treat essential hypertension. The primary end points included a measurement of peripheral endothelial function by brachial flow mediated vasodilatation (FMD) or forearm blood flow (FBF). A random-effect model was used to perform the meta-analysis when the studies showed significant heterogeneity, otherwise a descriptive analysis was conducted. Ten studies (689 patients) were included in qualitative analysis, four of which were included in quantitative synthesis. Meta-analysis showed that the changed FMD value before and after treatment with nebivolol was not statistically different from those treated with other ß-blockers [mean difference = 1.12, 95% confidence interval (CI): -0.56, 2.81, P=0.19]. Descriptive analysis indicated that nebivolol did not have a better endothelium-protective effect than other classes of antihypertensive drugs including olmesartan and perindopril. Nebivolol is not a unique endothelial function-protective agent distinguished from other ß-blockers or other classes of antihypertensive drugs. Reversal of endothelial dysfunction is a key point in the prevention and therapy of essential hypertension.

6.
BMC Med Genomics ; 13(1): 59, 2020 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-32252754

RESUMO

BACKGROUND: Escherichia coli are mostly commensals but also contain pathogenic lineages. It is largely unclear whether the commensal E. coli as the potential origins of pathogenic lineages may consist of monophyletic or polyphyletic populations, elucidation of which is expected to lead to novel insights into the associations of E. coli diversity with human health and diseases. METHODS: Using genomic sequencing and pulsed field gel electrophoresis (PFGE) techniques, we analyzed E. coli from the intestinal microbiota of three groups of healthy individuals, including preschool children, university students, and seniors of a longevity village, as well as colorectal cancer (CRC) patients, to probe the commensal E. coli populations for their diversity. RESULTS: We delineated the 2280 fresh E. coli isolates from 185 subjects into distinct genome types (genotypes) by PFGE. The genomic diversity of the sampled E. coli populations was so high that a given subject may have multiple genotypes of E. coli, with the general diversity within a host going up from preschool children through university students to seniors. Compared to the healthy subjects, the CRC patients had the lowest diversity level among their E. coli isolates. Notably, E. coli isolates from CRC patients could suppress the growth of E. coli bacteria isolated from healthy controls under nutrient-limited culture conditions. CONCLUSIONS: The coexistence of multiple E. coli lineages in a host may help create and maintain a microbial environment that is beneficial to the host. As such, the low diversity of E. coli bacteria may be associated with unhealthy microenvironment in the intestine and hence facilitate the pathogenesis of diseases such as CRC.

7.
Sci Rep ; 10(1): 5883, 2020 Apr 03.
Artigo em Inglês | MEDLINE | ID: mdl-32246038

RESUMO

Most diseases might be associated with acute or chronic inflammation, and the role of vitamin D in diseases has been extensively explored in recent years. Thus, we examined the associations of one of the best markers for inflammation - C-reactive protein (CRP) with 25-hydroxyvitamin D [25(OH)D] in 24 specific diseases. We performed cross-sectional analyses among 9,809 subjects aged ≥18 years who participated in the U.S. National Health and Nutrition Examination Survey (NHANES) in 2007~2010. The generalized additive model (GAM) was used to explore the associations of CRP with 25(OH)D in different diseases, adjusted for the age, gender, examination period and race. Distributions of CRP were significantly different (P < 0.05) in gender, examination period and race, and distributions of 25(OH)D were different (P < 0.05) in the examination period and race. Generally, CRP was negatively associated with 25(OH)D for majority diseases. 25(OH)D was negatively associated with CRP generally, and the associations were disease-specific and disease category-specific. In respiratory, gastrointestinal and mental diseases, the associations tended to be approximately linear. While in metabolic diseases, the associations were nonlinear, and the slope of the nonlinear curve decreased with 25(OH)D, especially when 25(OH)D < 30 µg/L.

8.
Antiviral Res ; 177: 104760, 2020 May.
Artigo em Inglês | MEDLINE | ID: mdl-32135219

RESUMO

Infection by RNA viruses such as human immunodeficiency virus (HIV)-1, influenza, and dengue virus (DENV) represent a major burden for human health worldwide. Although RNA viruses replicate in the infected host cell cytoplasm, the nucleus is central to key stages of the infectious cycle of HIV-1 and influenza, and an important target of DENV nonstructural protein 5 (NS5) in limiting the host antiviral response. We previously identified the small molecule ivermectin as an inhibitor of HIV-1 integrase nuclear entry, subsequently showing ivermectin could inhibit DENV NS5 nuclear import, as well as limit infection by viruses such as HIV-1 and DENV. We show here that ivermectin's broad spectrum antiviral activity relates to its ability to target the host importin (IMP) α/ß1 nuclear transport proteins responsible for nuclear entry of cargoes such as integrase and NS5. We establish for the first time that ivermectin can dissociate the preformed IMPα/ß1 heterodimer, as well as prevent its formation, through binding to the IMPα armadillo (ARM) repeat domain to impact IMPα thermal stability and α-helicity. We show that ivermectin inhibits NS5-IMPα interaction in a cell context using quantitative bimolecular fluorescence complementation. Finally, we show for the first time that ivermectin can limit infection by the DENV-related West Nile virus at low (µM) concentrations. Since it is FDA approved for parasitic indications, ivermectin merits closer consideration as a broad spectrum antiviral of interest.

9.
Int Ophthalmol ; 40(6): 1487-1499, 2020 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-32080793

RESUMO

OBJECTIVE: Shared decision making and meaningful patient involvement are key in improving cataract treatment outcomes, but no decision aid has been formally developed and validated for this purpose. Our aims were to develop a patient decision aid to guide patients' decision about when to undergo cataract surgery, and to determine patient's comprehension and booklet's acceptability. METHODS: The patient decision aid was developed and included evidence-based information about general cataract, its benefits, risks of treatment options, and value clarification exercise. A total of 30 patients with age-related cataract aged between 50 and 80 years were interviewed after using either the patient decision aid (n = 15) or the traditional education booklet (n = 15). RESULTS: The patients who received the decision aid agreed that the information was new (n = 15, 100%), the length of the aid was "just about right" (n = 13, 87%), the information was clear and easy to understand (n = 13, 87%), the decision aid was helpful in making decision (n = 13, 87%) and would like to recommend this decision aid to others (n = 14, 93%). CONCLUSIONS: The decision aid was assessed positively by patients with age-related cataract. There is a need for its further verification in the context of primary eye care setting.

10.
Chemistry ; 2020 Feb 10.
Artigo em Inglês | MEDLINE | ID: mdl-32039508

RESUMO

Successful synthesis of three kinds of dynamically stable compounds by a simple grinding method is reported, giving Ag6 Mo7 O24 , Ag-BTC (Ag-MOF, BTC=benzene-1,3,5-tricarboxylic acid), and {Ag6 Mo7 O24 }@Ag-MOF metal-organic frameworks (MOFs). According to the electrochemical dynamic analysis, these materials have pseudocapacitor behavior and high capacitance. The unique nanorod structure of {Ag6 Mo7 O24 }@Ag-MOF provides more active sites, faster ion/electron transfer, and electrolyte diffusion pathways, resulting in excellent specific capacitance (971 F g-1 ) higher than the other compounds. {Ag6 Mo7 O24 }@Ag-MOF (glassy carbon electrode) also has an excellent rate ability (60.1 %) and long cycle stability (98 % retention after 5000 cycles). In addition, the fully symmetrical button battery (with nickel foam as the current collector) fabricated with {Ag6 Mo7 O24 }@Ag-MOF delivers an energy density of 11.1 Wh kg-1 at 600.1 Wh kg-1 coupled with excellent cycling stability (86.4 %) at 1.2 V. These results demonstrate a new simple grinding method to prepare polyoxometalate-based metal-organic frameworks (POMOFs) for high-performance materials.

11.
BMC Oral Health ; 20(1): 9, 2020 Jan 08.
Artigo em Inglês | MEDLINE | ID: mdl-31914978

RESUMO

BACKGROUND: This study sought to evaluate dental utilization among 3-,4-, and 5-year-old children in China and to use Andersen's behavioural model to explore influencing factors, thereby providing a reference for future policy making. METHODS: This study is a cross-sectional study. Data of 40,305 children aged 3-5 years were extracted from the Fourth National Oral Health Survey, which was performed from August 2015 to December 2016. Patient data were collected using a questionnaire, which was answered by the child's parents, and clinical data were collected during a clinical examination. Stratification and survey weighting were incorporated into the complex survey design. Descriptive statistics, bivariate correlations and hierarchical logistic regression results were then analysed to find the factors associated with oral health service utilization. RESULTS: The oral health service utilization prevalence during the prior 12 months were 9.5% (95%CI: 8.1-11.1%) among 3-year-old children, 12.1% (95%CI: 10.8-13.5%) among 4-year-old children, and 17.5% (95%CI: 15.6-19.4%) among 5-year-old children. "No dental diseases" (71.3%) and "dental disease was not severe" (12.4%) were the principal reasons why children had not attended a dental visit in the past 12 months. The children whose parents had a bachelor's degree or higher (OR: 2.29, 95%CI: 1.97-2.67, p < 0.001), a better oral health attitude ranging from 5 to 8(OR: 1.64, 95%CI: 1.43-1.89, p < 0.001), annual per capital income more than 25,000 CNY (OR: 1.40, 95%CI: 1.18-1.65, p < 0.001),think their child have worse or bad oral health (OR: 3.54, 95%CI: 2.84-4.40, p < 0.001), and children who often have toothaches (OR: 9.72, 95%CI: 7.81-12.09, p < 0.001) were more likely to go to the dentist in the past year. CONCLUSION: The prevalence of dental service utilization was relatively low among preschool children. It is necessary to strengthen oral health education for parents and children, thereby improving oral health knowledge as well as attitude, and promoting dental utilization.

12.
Community Dent Oral Epidemiol ; 48(1): 32-41, 2020 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-31621099

RESUMO

OBJECTIVES: To explore the factors associated with utilization of oral health services among Chinese adults and older adults according to the Andersen Behavior Model. METHODS: Data from the 4th National Oral Health Survey (2015-2016) in China were used. A total of 7206 people (3669 adults aged 35-44 years and 3537 older adults aged 65-74 years) were included in our analysis. Oral health service utilization in the past 12 months was the outcome variable. Explanatory variables were selected according to the Andersen Behavior Model. Descriptive statistics and bivariate associations (chi-square tests) were analysed, followed by hierarchical Poisson regression models, which were conducted to determine the factors associated with oral health service utilization. RESULTS: In total, 21.4% (95% CI: 19.4%-23.7%) of adults (35-44 years old) and 20.7% (95% CI: 18.6%-22.9%) of older adults (65-74 years old) utilized oral health services in the past 12 months. Nearly 80% of adults (78.7%, 95% CI: 74.0%-82.7%) and more than 90% of older adults (93.7%, 95% CI: 91.0%-95.6%) visited a dentist for treatment. Adults aged 35-44 years old who were female (IRR: 1.15, 95% CI: 1.00-1.33, P = .047), had good oral health knowledge and attitudes (IRR: 1.30, 95% CI: 1.06-1.59, P = .011), perceived their oral health status as fair (IRR:1.51, 95% CI:1.24-1.85, P < .001) or poor/very poor (IRR:2.52, 95% CI:2.01-3.18, P < .001) and had a decayed, missing and filled teeth (DMFT) index >0 (IRR: 1.52, 95% CI: 1.11-2.09, P = .009) were more likely to report dental visits in the past 12 months. Older adults who utilized oral health services tended to be female (IRR: 1.32, 95% CI: 1.09-1.59, P = .004); to be covered by Urban Resident Basic Medical Insurance (URBMI) (IRR: 1.56, 95% CI: 1.18-2.05, P = .002), Urban Employee Basic Medical Insurance (UEBMI) (IRR: 1.69, 95% CI: 1.32-2.16, P < .001) or government medical insurance (GMI) (IRR: 1.03, 95% CI:1.01-2.16, P = .044); to have a high education level (IRR: 1.37, 95% CI: 1.08-1.74, P = .010); to have an income level in the 2nd tertile (IRR: 1.44, 95% CI: 1.13-1.84, P = .003) or 3rd tertile (IRR:1.52, 95% CI:1.18-1.95, P = .001); and to perceive their oral health status as poor or very poor (IRR: 1.53, 95% CI: 1.21-1.95, P = .001). CONCLUSIONS: Sex and self-perceived oral health status were associated with oral health utilization among Chinese population. Additionally, for older adults, education level, household income and insurance coverage were determinants of dental service use. These findings can aid in creating more targeted policies to increase the use of dental services by Chinese adults.

13.
Soc Sci Med ; 245: 112717, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31837634

RESUMO

Hospital violence has become a worldwide issue that disturbs health care systems. China is in a particular dilemma between meeting the healthcare demand of 1.39 billion residents and ensuring the safety of 12.3 million health professionals. Drawing on data from an administrative survey, we presented the types and distribution of disruptive behaviors, as well as the summary statistics of 225 medical disputes that took place from 2012 to 2013 in Z city. Logit and OLS regression analyses show that disruptive behaviors, characterized by the number of protest participants and the length of protest, can significantly predict whether the claimant receives compensation and the amount of compensation. All else equal, a one-person increase in the number of participants is associated with 3.94% higher odds of getting compensated, whereas a one-day increase in the length of protest is associated with a 1.03% increase in the odds of receiving compensation. Further analyses show that the link between disruptive behaviors and compensation outcomes is due to the involvement of the state, which tends to press hospitals to pay when substantial violence is present. Chinese government's overwhelming emphasis on social stability gives protestors leverage against hospitals, which can be summarized as "the squeaky wheel gets the grease." Ironically, the sensitivity of Chinese government towards social stability becomes a weakness of its own. Government's active intervention to reach a peace-oriented goal will incentivize patients to resort to violence in pursuit of compensation. This study has implications for understanding the Chinese government's logic of addressing social problems that range from hospital violence, labor disputes, land disputes to demolition compensation and civil petitions.

14.
J Exp Bot ; 71(6): 1842-1857, 2020 Mar 25.
Artigo em Inglês | MEDLINE | ID: mdl-31875914

RESUMO

Drought-response-element binding (DREB)-like transcription factors can significantly enhance plant tolerance to water stress. However, most research on DREB-like proteins to date has been conducted in growth chambers or greenhouses, so there is very little evidence available to support their practical use in the field. In this study, we overexpressed GmDREB1 from soybean in two popular wheat varieties and conducted drought-tolerance experiments across a range of years, sites, and drought-stress regimes. We found that the transgenic plants consistently exhibited significant improvements in yield performance and a variety of physiological traits compared with wild-type plants when grown under limited water conditions in the field, for example showing grain yield increases between 4.79-18.43%. Specifically, we found that the transgenic plants had reduced membrane damage and enhanced osmotic adjustment and photosynthetic efficiency compared to the non-transgenic controls. Three enzymes from the biosynthetic pathway of the phytohormone melatonin were up-regulated in the transgenic plants, and external application of melatonin was found to improve drought tolerance. Together, our results demonstrate the utility of transgenic overexpression of GmDREB1 to improve the drought tolerance of wheat in the field.

15.
Food Res Int ; 126: 108582, 2019 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-31732056

RESUMO

Vitis davidii Föex is widely planted in South China as a potential wine making grape. In this study, yeast communities during the spontaneous fermentations of two varieties of Vitis davidii Föex in Guizhou, China were investigated. Hanseniaspora uvarum, Pichia terricola, Saccharomyces cerevisiae/S. mikatae, and Schizosaccharomyces japonicus were the four main yeast species detected by culture-dependent and high-throughput sequencing approaches. However, the composition of minor yeast species was quite different as revealed by the two approaches. Ten yeast species including Debaryomyces hansenii, Rhodotorula mucilaginosa, Sporidiobolus paraoseus, Starmerella bacillaris, Zygoascus meyerae, etc. were detected by culture-dependent approaches, whereas the other five species were found by high-throughput sequencing analysis. S. japonicus was widely found during spontaneous fermentations and its concentrations were mostly higher than that of S. cerevisiae. The difference in grape varieties and fermentation conditions contributed to yeast diversity. The results of this study provide basic information on indigenous yeast diversity from Vitis davidii Föex in Guizhou, which would help to exploit the potential of non-Saccharomyces yeast species with good oenological attributes.

16.
Int J Mol Med ; 2019 Jul 11.
Artigo em Inglês | MEDLINE | ID: mdl-31531678

RESUMO

Following the publication of the above article, an interested reader drew to our attention a number of issues and concerns related to the descriptions of the genes and mouse models in the above paper. After having consulted with the authors, they have acknowledged that their paper did indeed contain a number of errors, and these are listed below in this Corrigendum. First, the authors realized that an incorrect figure was included as Fig. 1 in the paper. The correct (and entirely different) version of Fig. 1, together with its figure legend, is shown opposite. The following textual errors in the paper should also be noted: i) In the Abstract, lines 3­4 in the left­hand column (LHC): "androgen receptor (AR)­/­, AR+/­ and AR+/+ male mice" should have been written as "androgen receptor (AR) conditional knockout mice"; line 5 in the LHC: "flox­AR" should have been written as "ARflox/+"; line 6 in the LHC: "AR­Cre" should have been written as "Amhr2­Cre"; line 22 in the LHC: "AR+/­" should have been written as "ARflox/Y"; line 1 in the right­hand column (RHC): "heterozygous" should have been written as "ARflox/Y"; and line 2 in the RHC, "heterozygous" should have been written as "ARflox/Y Cre+". ii) In the Introduction, paragraph 3, lines 6­7 in the LHC: "or female ARKO mice" should have been deleted; paragraph 4, line 5 in the LHC: "AR­/­", "AR+/­" and "AR+/+" should have been deleted; paragraph 4, line 11 in the LHC: "AR­/­", and "AR+/­" should have been written as "AR­/Y", whereas "AR+/+" should have been written as "AR+/Y". iii) In the Materials and methods, paragraph 3, the last sentence in the RHC {"The identification of the plasmid containing leydig cells expressing Cre recombinant enzyme and the specificity of the genetically modified (gm) sequence [Amhr2­Cre (7.1)] are shown in Fig. 1} should have been written as "The positive clones were identified by digestion and sequencing." iv) In the Results, the first subheading in the RHC ("...transgenic mouse model (Amhr2­Cre)" should have been written as "...AR conditional knockout targeting vector"; paragraph 1, lines 1­3 in the RHC: The sentence "The AR gene condition knockout plasmid was identified and confirmed by enzyme digestion with BamHI" should have been written as "The AR gene conditional knockout strategy is shown in Fig. 1. The gene targeting vector was identified and confirmed by enzyme digestion with BamHI"; paragraph 1, line 4 in the RHC: "2.7 kb" should have been written as "7.8 kb"; Table III, in the title and in the Table heading, "AR+/­" should have been written as "AR+/Y", and "AR­/­" should have been written as "AR­/Y"; in the subheading "Comparison between AR­/­ male mice and normal mice", "AR­/­" should have been written as "AR­/Y", and "normal" should have been written as "AR+/Y"; and paragraph 4, lines 3­5 in the LHC: "AR+/­" should have been written as "AR+/Y", and "AR­/­" should have been written as "AR­/Y". All the authors thank the interested reader for drawing these matters to their attention, and agree to the contents of this Corrigendum. The authors also stress that these errors did not significantly influence either the results or the conclusions of the paper. Furthermore, the authors apologize to the Editor of International Journal of Molecular Medicine and to the readership for any inconvenience caused. [the original article was published in International Journal of Molecular Medicine 35: 399­404, 2015; DOI: 10.3892/ijmm.2014.2015].

17.
ISA Trans ; 2019 Sep 02.
Artigo em Inglês | MEDLINE | ID: mdl-31492469

RESUMO

This article concentrates on an adaptive backstepping control design of time-delay strict-feedback uncertain nonlinear systems subject to full state constraints. The tan-type barrier Lyapunov functions (tBLFs) and Lyapunov-Krasovskii function are united together, which successfully get over the difficulties of system design in which the first function is involved to ensure full state constraints satisfaction and the second is established to eliminate the effect of delayed states. By employing a new control scheme, asymptotic tracking performance is arrived, and all the states remain in the desirable regions for all the system running time. Meanwhile, the boundedness of all signals of the closed-loop system is guaranteed. The performance of the control scheme is illustrated through a class of single degree of freedom (1-DOF) time-delay electrostatic microactuator systems.

18.
Microbiologyopen ; 8(12): e922, 2019 12.
Artigo em Inglês | MEDLINE | ID: mdl-31482696

RESUMO

The genus Laceyella consists of a thermophilic filamentous bacteria. The pure isolate of Laceyella sacchari FBKL4.010 was isolated from Moutai-flavor Daqu, Guizhou Province, China. In this study, the whole genome was sequenced and analyzed. The complete genome consists of one 3,374,379-bp circular chromosome with 3,145 coding sequences (CDSs), seven clustered regularly interspaced short palindromic repeat (CRISPR) regions of 12 CRISPRs. Moreover, we identified that the genome contains genes encoding key enzymes such as proteases, peptidases, and acetolactate synthase (ALS) of the tetramethylpyrazine metabolic pathway. Metabolic pathways relevant to tetramethylpyrazine synthesis were also reconstructed based on the Kyoto Encyclopedia of Genes and Genomes (KEGG) PATHWAY database. Annotation and syntenic analyses using antiSMASH 4.0 also revealed the presence of two gene clusters in this strain that differ from known tetramethylpyrazine synthesis clusters, with one encoding amino acid dehydrogenase (ADH) and the other encoding transaminase in tetramethylpyrazine metabolism. The results of this study provide flavor and genomic references for further research on the flavor-producing functions of strain FBKL4.010 in the Moutai liquor-making process.

19.
Nanomaterials (Basel) ; 9(6)2019 Jun 21.
Artigo em Inglês | MEDLINE | ID: mdl-31234460

RESUMO

Graphene as a two-dimensional (2D) nanoplatform is beneficial for assembling a 2D heterojunction photocatalytic system to promote electron transfer in semiconductor composites. Here a BiVO4 nanosheets/reduced graphene oxide (RGO) based 2D-2D heterojunction photocatalytic system as well as 0D-2D BiVO4 nanoparticles/RGO and 1D-2D BiVO4 nanotubes/RGO nanocomposites are fabricated by a feasible solvothermal process. During the synthesis; the growth of BiVO4 and the intimate interfacial contact between BiVO4 and RGO occur simultaneously. Compared to 0D-2D and 1D-2D heterojunctions, the resulting 2D-2D BiVO4 nanosheets/RGO composites yield superior chemical coupling; leading to exhibit higher photocatalytic activity toward the degradation of acetaminophen under visible light irradiation. Photoluminescence (PL) and photocurrent experiments revealed that the apparent electron transfer rate in 2D-2D BiVO4 nanosheets/RGO composites is faster than that in 0D-2D BiVO4 nanoparticles/RGO composites. The experimental findings presented here clearly demonstrate that the 2D-2D heterojunction interface can highlight the optoelectronic coupling between nanomaterials and promote the electron-hole separation. This study will motivate new developments in dimensionality factors on designing the heterojunction photocatalysts and promote their photodegradation photocatalytic application in environmental issues.

20.
Onco Targets Ther ; 12: 3327-3338, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31118684

RESUMO

Background: Tobacco is a major risk factor for oral squamous cell carcinoma (OSCC). However, the role of nicotine in OSCC is not completely understood. Materials and methods: To analyze the mechanisms of nicotine-induced cervical metastasis, we investigated whether nicotine induced invasion, migration, and epithelial-mesenchymal transition (EMT) via regulating peroxiredoxin 1 (Prx1) in CAL 27 cells. In addition, we established a mouse model to confirm the roles of nicotine in regulating Ets1/Prx1/EMT signaling in OSCC metastasis. Results: We showed that nicotine induced CAL 27 cell invasion, migration, EMT, and Prx1 and Ets1 expression. Prx1 knockdown inhibited cell invasion, migration, and EMT. Ets1 silencing downregulated Prx1 expression and EMT. Prx1 and Ets1 were shown to interact in CAL 27 cells treated with nicotine, and nicotine could significantly upregulate the binding of the transcription factor Ets1 to the Prx1 gene promoter region. Additionally, an in vivo study showed that nicotine induced tumor metastasis and EMT. Prx1 knockdown inhibited cervical metastasis rates and EMT progression. No significant differences in metastasis rates and EMT-related marker expression levels were observed between vehicle- and nicotine-treated mice. Conclusion: The results indicate that nicotine promotes cervical lymph node metastasis through regulating Ets1/Prx1/EMT signaling during OSCC pathogenesis; consequently, Prx1 may represent a potential target for the prevention and treatment of OSCC.

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