Your browser doesn't support javascript.
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 21
Filtrar
Mais filtros










Base de dados
Intervalo de ano de publicação
1.
BMC Cancer ; 19(1): 1054, 2019 Nov 06.
Artigo em Inglês | MEDLINE | ID: mdl-31694589

RESUMO

BACKGROUND: Bladder cancer (BC) has become a major worldwide public health issue, especially non-muscle-invasive bladder cancer (NMIBC). A flood of related clinical practice guidelines (CPGs) have emerged; however, the quality and recommendations of the guidelines are controversial. We aimed to appraise the quality of the CPGs for NMIBC within the past 5 years and compare the similarities and differences between recommendations for therapies. METHODS: A systematic search to identify CPGs for NMIBC was performed using electronic databases (including PubMed, Embase, Web of Science), guideline development organizations, and professional societies from January 12, 2014 to January 12, 2019. The Appraisal of Guidelines Research & Evaluation (AGREE) II instrument was used to evaluate the quality of the guidelines. Intraclass correlation coefficient (ICC) analysis was performed to assess the overall agreement among reviewers. RESULTS: Nine CPGs were included. The overall agreement among reviewers was excellent. The interquartile range (IQR) of scores for each domain were as follows: scope and purpose 69.44% (35.42, 85.42%); stakeholder involvement 41.67% (30.56, 75.00%); rigour of development 48.96% (27.08, 65.63%); clarity and presentation 80.56% (75.00, 86.11%); applicability 34.38% (22.92, 40.63%) and editorial independence 70.83% (35.42, 85.42%). The NICE, AUA, EAU and CRHA/CPAM clinical practice guidelines consistently scored well in most domains. It was generally accepted that the transurethral resection of bladder tumour (TURBT) and intravesical chemotherapy should be performed in the management of bladder cancer. The application of chemotherapy was highly controversial in high risk NMIBC. The courses of BCG maintenance were similar and included 3 years of therapy at full maintenance doses. CONCLUSIONS: The quality of NMIBC guidelines within the past 5 years varied, especially regarding stakeholders, rigour and applicability. Despite many similarities, the recommendations had some inconsistencies in the details.

2.
Se Pu ; 37(11): 1209-1214, 2019 Nov 08.
Artigo em Chinês | MEDLINE | ID: mdl-31642274

RESUMO

Radial thin-layer chromatography (RTLC) is a form of chromatography in which the sample and developing agent/solvent are applied at the edge of a circle on the silica-layered TLC plate. The sample components are radially transported from the center of the circle toward the periphery by the solvent flow. RTLC is an efficient separation and semi-preparation method, with the advantages of fast separation, low diffusion, and absence of tailing at a certain distance. Although RTLC isolation has been well documented in the literature, there are only a few qualitative and quantitative reports on this technique. In this study, a self-programming module combined with image processing software was adopted for digital processing of RTLC images, and a digital atlas was created. Qualitative and quantitative verification of RTLC was then conducted. With the aid of computer technology, qualitative and quantitative methods of radially expanded TLC for Yiqing tablets were investigated. The results showed that the qualitative RTLC method has good reliability, but the quantitative RTLC method has inherent drawbacks, which may be associated with the experimental environment. We strongly believe that better separation and data acquisition equipment, as well as the combination of computer image processing and programming methods, will open up infinite possibilities of analysis/separation using RTLC-based techniques. We also believe that TLC will take a new step in the future with the assistance of computer technology.


Assuntos
Cromatografia em Camada Delgada/instrumentação , Medicamentos de Ervas Chinesas/análise , Processamento de Imagem Assistida por Computador , Reprodutibilidade dos Testes , Solventes , Comprimidos
3.
4.
J Colloid Interface Sci ; 542: 81-90, 2019 Apr 15.
Artigo em Inglês | MEDLINE | ID: mdl-30735890

RESUMO

Three-dimensional reduced graphene oxide@SnO2@nitrogen-doped carbon (3DG@SnO2@N-C) composites are designed as high efficiency anode materials for lithium-ion batteries. The SnO2 particle size, surface area and pore size distribution of the 3DG@SnO2@N-C could be simply controlled by altering the GO dosages. The optimized 3DG@SnO2@N-C electrode demonstrates a reversible capacity of 1349.5 mAh g-1 after 100 cycles at the current density of 100 mA g-1. Based on the structural and electrochemical dynamic tests, the bi-functional N-doped carbon coating layer could serve as both conductive channel for electron transport and as buffer layer to alleviate the volume change of embedded SnO2 NPs. In addition, the cross-linked conducting 3DG with porous structure attributes to the reduced electron transport and Li ion diffusion resistances, which finally leads to the enhanced cycling stability and rate performances.

5.
Anal Bioanal Chem ; 411(11): 2273-2282, 2019 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-30806752

RESUMO

Coronary artery disease (CAD) is a manifestation of systemic atherosclerotic disease. It is assessed by intervention or traditional scoring risk factors. Diagnosis is limited by inaccurate and invasive methods. Developing noninvasive methods to screen for the risk of CAD is a major challenge. We aimed to identify urinary proteins associated with CAD. We utilized iTRAQ labeling followed by 2D LC-MS/MS to compare the urinary proteome of CAD patients to healthy cohorts. The multiple reaction monitoring (MRM) was used to verify the differential proteins. ROC analysis based on MRM data was used to evaluate the diagnostic application. A total of 876 proteins were quantified, and 100 differential proteins were found. Functional analysis revealed that the differential proteins were mainly associated with Liver X Receptor/Retinoid X Receptor (LXR/RXR) pathway activation, atherosclerosis signaling, production of nitric oxide and reactive oxygen species, and the top upstream regulator of the differential proteins by IPA analysis indicated to the APOE. Nineteen differential proteins were verified by MRM analysis. ROC based on MRM data revealed that the combination of two proteins (APOD and TFF1) could diagnose CAD with 85% sensitivity and 99% specificity (AUC 0.95). The urinary proteome might reflect the pathophysiological changes in CAD and be used for the clinical study of CAD.


Assuntos
Doença da Artéria Coronariana/diagnóstico , Doença da Artéria Coronariana/urina , Proteinúria/urina , Proteômica/métodos , Espectrometria de Massas em Tandem/métodos , Urinálise/métodos , Adulto , Aterosclerose/diagnóstico , Aterosclerose/urina , Biomarcadores/urina , Feminino , Humanos , Masculino , Pessoa de Meia-Idade
6.
J Colloid Interface Sci ; 539: 194-202, 2019 Mar 15.
Artigo em Inglês | MEDLINE | ID: mdl-30580175

RESUMO

TiO2 microsphere with tunable pore and chamber size are prepared by a simple solventhermal method and used as catalyst for the photocatalytic CO2 reduction. It is found that the hollow microsphere with relative lower surface area of 73.8 m2 g-1 exhibits increased pore size of 18.1 nm and cavity structure, leading to higher CO2 diffusion coefficient of 5.40 × 10-5 cm2 s-1 compared with the solid and yolk/shell microspheres. Therefore, the hollow microsphere possesses more accessible sites for CO2 adsorption, which finally gives rise to the enhanced CO production rate of 10.9 ±â€¯0.7 µmol g-1 h-1 under simulated sunlight, which is respectively 1.6 and 1.4 times higher than that of solid and yolk/shell microspheres. Electron dynamic study further demonstrates that hollow microsphere shows the highest photocurrent density and the lowest charge recombination among three microspheres structure, which is attributed to the swift CO2 diffusion providing fresh CO2 molecules to rapidly scavenge the photo-generated electrons and finally leading to the excellence catalytic reduction performances. This method could be adopted as a general strategy to prepare high performance TiO2 catalysts with desirable structural qualities for the photocatalytic CO2 reduction under nature sunlight.

7.
J Colloid Interface Sci ; 533: 539-547, 2019 Jan 01.
Artigo em Inglês | MEDLINE | ID: mdl-30179832

RESUMO

Janus shaped BiOCl/MoS2 composites with two dimensional configuration are successfully prepared via a facile pulse ultrasonic assisted method, which spontaneously introduces oxygen vacancies on the BiOCl surface and builds well-defined heterojuction at the BiOCl/MoS2 interfaces. The as-prepared BiOCl/MoS2 composites possess reduced band gap and defect energy levels due to the incorporation of MoS2 and the oxygen vacancies, which permits the enhanced light harvesting efficiency in the visible range. In addition, because of the formed BiS bonds at the BiOCl/MoS2 interface, the composites demonstrate improved charge separation of the photo-generated carriers. Therefore, when used as photocatalyst for Rhodamine B photodegradation, the optimized composite demonstrates a degradation rate of 0.078 min-1, which is much enhanced compared with that of pure BiOCl (0.052 min-1). Mechanism investigation indicates the degradation is a hole mediated process. In addition, the composite shows good stability and outstanding organic carbon removal efficiency, which could serve as a promising photocatalyst for water remediation under visible light.

8.
Langmuir ; 34(37): 11195-11203, 2018 09 18.
Artigo em Inglês | MEDLINE | ID: mdl-30133297

RESUMO

Coalescence-induced droplet self-jumping on superhydrophobic surfaces has received extensive attentions over the past decade because of its potential applications ranging from anti-icing materials to self-sustained condensers, in which a higher jumping velocity vj is always expected and favorable. However, the previous studies have shown that there is a velocity limit with vj ≤ 0.23 uic for microscale droplets and vj ≤ 0.127 uic for nanoscale droplets, where uic is referred to as the inertial-capillary velocity. Here, we show that the jumping velocity can be significantly increased by patterning a single groove, ridge, or more hydrophobic strip, whose size is comparable with the radius of coalescing droplets, on a superhydrophobic surface. We implement molecular dynamics simulations to investigate the coalescence of two equally sized nanodroplets (8.0 nm in radius) on these surfaces. We found that a maximum vj = 0.23 uic is achieved on the surface with a 1.6 nm high and 5.9 nm wide ridge, which is 1.81 times higher than the nanoscale velocity limit. We also demonstrate that the presence of groove, ridge, and strip alters coalescence dynamics of droplets, leading to a significantly shortened coalescence time which remarkably reduces viscous dissipation during coalescence; thus, we believe that the present approach is also effective for microscale droplet jumping.

9.
Microsc Microanal ; 24(4): 420-423, 2018 08.
Artigo em Inglês | MEDLINE | ID: mdl-29925461

RESUMO

Electron backscattered diffraction (EBSD) is a technique regularly used to obtain crystallographic information from inorganic samples. When EBSD is acquired simultaneously with emitting diodes data, a sample can be thoroughly characterized both structurally and compositionally. For organic materials, coherent Kikuchi patterns do form when the electron beam interacts with crystalline material. However, such patterns tend to be weak due to the low average atomic number of organic materials. This is compounded by the fact that the patterns fade quickly and disappear completely once a critical electron dose is exceeded, inhibiting successful collection of EBSD maps from them. In this study, a new approach is presented that allows successful collection of EBSD maps from organic materials, here the extreme example of a hydrocarbon organic molecular thin film, and opens new avenues of characterization for crystalline organic materials.

10.
PLoS One ; 12(5): e0177578, 2017.
Artigo em Inglês | MEDLINE | ID: mdl-28498864

RESUMO

Green material selection is a crucial step for the material industry to comprehensively improve material properties and promote sustainable development. However, because of the subjectivity and conflicting evaluation criteria in its process, green material selection, as a multi-criteria decision making (MCDM) problem, has been a widespread concern to the relevant experts. Thus, this study proposes a hybrid MCDM approach that combines decision making and evaluation laboratory (DEMATEL), analytical network process (ANP), grey relational analysis (GRA) and technique for order performance by similarity to ideal solution (TOPSIS) to select the optimal green material for sustainability based on the product's needs. A nonlinear programming model with constraints was proposed to obtain the integrated closeness index. Subsequently, an empirical application of rubbish bins was used to illustrate the proposed method. In addition, a sensitivity analysis and a comparison with existing methods were employed to validate the accuracy and stability of the obtained final results. We found that this method provides a more accurate and effective decision support tool for alternative evaluation or strategy selection.


Assuntos
Tomada de Decisões , Técnicas de Apoio para a Decisão , Modelos Teóricos , Gerenciamento de Resíduos
11.
ACS Appl Mater Interfaces ; 8(20): 13051-7, 2016 05 25.
Artigo em Inglês | MEDLINE | ID: mdl-27144401

RESUMO

Both inorganic and polymeric aerogels are well-known in the materials field. Inorganic aerogels are generally susceptible to brittle fracture, while polymeric aerogels tend to exhibit low modului and high flammability. To overcome these disadvantages, we introduce a new approach to the design of aerogels. A microporous poly(vinyl alcohol) (PVA) aerogel/silica nanocomposite was prepared by growing a silica conformal coating onto a PVA aerogel scaffold. Such aerogel/silica nanocomposites show significant improvement in their mechanical properties over either individual component. The nanocomposites show excellent fire resistance since the silica conformal coating serves as a barrier for heat transfer and mass loss of the coated organic materials. After a fluorocarbon silane treatment, the nanocomposites also show durable superhydrophobicity.

12.
Toxicol Res (Camb) ; 5(5): 1400-1409, 2016 Sep 01.
Artigo em Inglês | MEDLINE | ID: mdl-30090444

RESUMO

Developmental exposure to bisphenol A (BPA) has been linked to impaired glucose homeostasis and pancreatic function in adulthood, which has been hypothesized to result from the disruption of pancreatic ß-cell development at early life. Here we evaluated whether maternal BPA exposure disrupts ß-cell development and glucose tolerance and the role of epigenetic modifications of key regulator in this process. We found that maternal exposure to BPA (10 µg kg-1 d-1) reduced the pancreatic ß-cell mass and the expression of pancreatic and duodenal homeobox 1 (Pdx1) at birth, as well as the expression of Pdx1 at gestational day (GD) 15.5. In parallel with the decreased expression of Pdx1, histones H3 and H4 deacetylation, along with demethylation of histone 3 lysine 4 (H3K4) and methylation of histone 3 lysine 9 (H3K9), were found at the promoter of Pdx1, while no significant changes in DNA methylation status were detected at this region. Moreover, these alterations were observed in adult life along with impaired glucose tolerance. We conclude that maternal exposure to BPA reduces pancreatic ß-cell mass at birth by reducing PDX1+ progenitors during fetal development through altering the histone modifications of Pdx1, which can be propagated to later life and increase the susceptibility to glucose intolerance.

13.
PLoS One ; 10(7): e0133270, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-26222143

RESUMO

Urine and cerebrospinal fluid (CSF) are two important biofluids used for disease biomarker discovery. For differential proteomic analysis, it is essential to evaluate individual and gender variations. In this study, we characterized urinary and CSF proteomes of 14 healthy volunteers with regard to individual and gender variations using 2DLC-MS/MS analysis and 8-plex iTRAQ quantification. A total of 968/512 urinary/CSF proteins were identified, with 406/280 quantified in all individuals. The median inter-individual coefficients of variation (CVs) were 0.262 and 0.183 for urinary and CSF proteomes, respectively. Cluster analysis showed that male and female urinary proteomes exhibited different patterns, though CSF proteome showed no remarkable gender differences. In comparison with CSF proteome, urinary proteome showed higher individual variation. Further analysis revealed that individual variation was not correlated with protein abundance. The minimum sample size for proteomic analysis with a 2-fold change was 10 (4/5 for males/females using iTRAQ quantification) for urinary or 8 for CSF proteome. Intracellular proteins leaked from exfoliative cells tended to have higher CVs, and extracellular proteins secreted from urinary tract or originating from plasma tended to have lower CVs. The above results might be beneficial for differential proteomic analysis and biomarker discovery.


Assuntos
Proteínas do Líquido Cefalorraquidiano/metabolismo , Proteoma/análise , Proteômica/métodos , Espectrometria de Massas em Tandem/métodos , Urinálise/métodos , Urina/química , Adulto , Cromatografia Líquida/métodos , Feminino , Ontologia Genética , Humanos , Marcação por Isótopo , Masculino , Fatores Sexuais , Transdução de Sinais , Adulto Jovem
14.
Psychol Rep ; 116(3): 751-67, 2015 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-25984942

RESUMO

Previous studies have used social learning theory to explain the influence of ethical leadership. This study continues the previous research by using social learning theory to explain the mediating effect of self-efficacy on the relationship between ethical leadership and employee voice. In addition, this study extends previous studies by introducing expectancy theory to explore whether self-impact also mediates the relationship between ethical leadership and employee voice. Ethical leadership, self-efficacy, self-impact, and employee voice were assessed using paired surveys among 59 supervisors and 295 subordinates employed at nine firms in the People's Republic of China. Using HLM and SEM analyses, the results revealed that ethical leadership was positively related to employee voice and that this relationship was partially mediated by both self-efficacy and self-impact.


Assuntos
Emprego/psicologia , Relações Interpessoais , Liderança , Autoeficácia , Adulto , China , Feminino , Humanos , Masculino , Cultura Organizacional
15.
Proteome Sci ; 13: 12, 2015.
Artigo em Inglês | MEDLINE | ID: mdl-25866482

RESUMO

BACKGROUNDS: Pseudoprogression disease (PsPD) is commonly observed during glioblastoma (GBM) follow-up after adjuvant therapy. Because it is difficult to differentiate PsPD from true early progression of GBM, we have used a quantitative proteomics strategy to identify molecular signatures and develop predictive markers of PsPD. RESULTS: An initial screening of three PsPD and three GBM patients was performed, and from which 530 proteins with significant fold changes were identified. By conducting biological functional analysis of these proteins, we found evidence that the protein synthesis network and the cellular growth and proliferation network were most significantly affected. Moreover, six of the proteins (HNRNPK, ELAVL1, CDH2, FBLN1, CALU and FGB) involved in the two networks were validated (n = 18) in the same six samples and in twelve additional samples using immunohistochemistry methods and the western blot analysis. The receiver operating characteristic (ROC) curve analysis in distinguishing PsPD patients from GBM patients yielded an area under curve (AUC) value of 0.90 (95% confidence interval (CI), 0.662-0.9880) for CDH2 and.0.92 (95% CI, 0.696-0.995) for CDH2 combined with ELAVL1. CONCLUSIONS: The results of the present study both revealed the biological signatures of PsPD from a proteomics perspective and indicated that CDH2 alone or combined with ELAVL1 could be potential biomarkers with high accuracy in the diagnosis of PsPD.

16.
J Biotechnol ; 203: 97-103, 2015 Jun 10.
Artigo em Inglês | MEDLINE | ID: mdl-25840366

RESUMO

In this work, we developed an immunosensor for electrochemical detection of penicillin G at trace level. The biosensor was fabricated by immobilizing anti-penicillin G in a supported bilayer lipid membrane (s-BLM) modified with gold nanoparticles, and the modified electrodes were characterized by the scanning electron microscope (SEM), cyclic voltammetry and electrochemical impedance spectroscopy. The biosensor was able to detect penicillin G with a linear correlation ranging from 3.34×10(-3)ng/L to 3.34×10(3)ng/L and a detection limit of 2.7×10(-4)ng/L, much lower than the maximum residue limit (MRL) of penicillin G in milk (4ppb, equal to 4×10(3)ng/L) set out by the European Union. The mean coefficient variation (CV) of the intra-assays and the inter-assays were 5.4% and 7.7%, respectively. In addition, the concentration of penicillin G in milk samples determined by this biosensor was in good agreement with that determined by high performance liquid chromatography (HPLC) assay.


Assuntos
Técnicas Biossensoriais , Leite/química , Penicilina G/análise , Animais , Anticorpos/imunologia , Colesterol/química , Espectroscopia Dielétrica , Eletrodos , Ouro/química , Bicamadas Lipídicas/química , Nanopartículas Metálicas/química , Nanopartículas Metálicas/ultraestrutura , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Penicilina G/imunologia , Fosfatidilcolinas/química
17.
Sheng Wu Gong Cheng Xue Bao ; 31(11): 1643-50, 2015 Nov.
Artigo em Chinês | MEDLINE | ID: mdl-26939447

RESUMO

To develop a timesaving and easy operating Reverse Phase (RP) chromatography method, we adopted Thermo Pierce RP C18 Tip to separate small amount hippocampus peptide mixtures and to compare with high performance liquid chromatography (HPLC). According to the separation performance of 4 ACN gradient optimization methods, we determined the best ACN concentration gradient. The results showed that, the experiment took only 10 min by separating with eight ACN concentration gradient, which accounted 1/4 for HPLC. But as for the identified proteins, RP C18 Tip accounted 85.5% for HPLC. ACN gradient of 5%, 15%, 20% and 90% had best repeatability (P = 0.429) and result for separating 30 µg peptides. This method is easy to operate, timesaving and has low cost. It could be used into pretreatment of small amount complex proteomic samples.


Assuntos
Cromatografia de Fase Reversa/métodos , Proteínas/isolamento & purificação , Proteômica/métodos , Cromatografia Líquida de Alta Pressão , Peptídeos
18.
Proteomics ; 14(16): 1922-32, 2014 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-24962155

RESUMO

Extracellular microvesicles (EVs) are membranous vesicles, which are released from diverse cells. These EVs have also been found in a wide range of body fluids. The cargo of EVs, including proteins, lipids, carbohydrates, and nucleic acids, can be stably preserved in EVs. Researchers have found that EVs can mediate intercellular communication by shuttling the cargo components. Therefore, EVs can be used for the identification of disease-specific biomarkers. As one class of EVs, urinary exosomes can reflect the status of the renal system. Moreover, urinary exosome analysis can minimize the interference of high abundant proteins in the whole urine sample. Therefore, urinary exosomes have gained much attention in recent years. In this review, we present a comprehensive summary of urinary exosome studies in recent years, including collection, storage, and isolation methods. The normal and disease proteomic analyses of urinary exosomes are also presented. Thus, this review may provide a valuable reference for future research.


Assuntos
Exossomos/química , Proteinúria/urina , Proteoma/análise , Urina/química , Animais , Humanos , Espectrometria de Massas/métodos , Proteômica/métodos
19.
Toxicol Lett ; 228(2): 85-92, 2014 Jul 15.
Artigo em Inglês | MEDLINE | ID: mdl-24768697

RESUMO

An emerging literature suggests that bisphenol A (BPA), a widespread endocrine disrupting chemical, when exposure occurs in early life, may increase the risk of metabolic syndrome. In this study, we investigated the hypothesis that perinatal exposure to BPA predisposed offspring to fatty liver disease: the hepatic manifestation of metabolic syndrome, and its possible mechanism. Pregnant Wistar rats were administered with BPA (40µg/kg/day) or vehicle during gestation and lactation. Liver histology, biochemical analysis, transcriptome, and mitochondrial function were examined in male offspring at postnatal 3, 15 and 26 weeks. At 3 weeks of age, abnormal liver morphology and function were not observed in the BPA-exposed offspring, but a decrease in mitochondrial respiratory complex (MRC) activity (I and III) and significant changes in gene expression involved in mitochondrial fatty acid metabolism were observed compared with controls. At 15 weeks, micro-vesicular steatosis in liver, up-regulated genes involved in lipogenesis pathways, increased ROS generation and Cytc release were observed in the BPA-exposed offspring. Then, extensive fatty accumulation in liver and elevated serum ALT were observed in BPA-exposed offspring at 26 weeks. In the longitudinal observation, hepatic mitochondrial function including MRC activity, ATP production, ROS generation and mitochondrial membrane potential were progressively worsened in the BPA-exposed offspring. Perinatal BPA exposure contributes to the development of hepatic steatosis in the offspring of rats, which may be mediated through impaired hepatic mitochondrial function and up-regulated hepatic lipid metabolism.


Assuntos
Compostos Benzidrílicos/efeitos adversos , Fígado Gorduroso/induzido quimicamente , Doenças Mitocondriais/induzido quimicamente , Fenóis/efeitos adversos , Trifosfato de Adenosina/metabolismo , Animais , Western Blotting , Peso Corporal , Citocromos c/metabolismo , Citosol/metabolismo , Ácidos Graxos não Esterificados/sangue , Fígado Gorduroso/patologia , Feminino , Hepatócitos/metabolismo , Lipídeos/biossíntese , Fígado/patologia , Masculino , Potencial da Membrana Mitocondrial/efeitos dos fármacos , Doenças Mitocondriais/patologia , Gravidez , Efeitos Tardios da Exposição Pré-Natal , Ratos , Ratos Wistar , Espécies Reativas de Oxigênio/metabolismo , Reação em Cadeia da Polimerase em Tempo Real , Triglicerídeos/sangue
20.
Chin Med J (Engl) ; 122(24): 2939-44, 2009 Dec 20.
Artigo em Inglês | MEDLINE | ID: mdl-20137478

RESUMO

BACKGROUND: The body mass index, airflow obstruction, dyspnea, and exercise capacity (BODE) index was shown at predicting the risk of death, exacerbation and disease severity among patients with COPD, but few studies verified relationship between BODE index and health related quality of life (HRQoL) among Chinese COPD patients. The objective of this study was to evaluate the relationship between BODE index and HRQoL in cross-sectional and longitudinal association analyses. METHODS: A multi-center prospective cohort study was initially conducted in 491 stable COPD patients in Beijing, China. Health status (HRQoL) was assessed by St. George's Respiratory Questionnaire (SGRQ); the BODE index was calculated for each patient; dyspnea was assessed using the 5-grade Medical Research Council dyspnea scale. Other measurements included socio-demographic, body mass index (BMI), lung function test and 6-minute-walk test (6MWT). Patients were then followed monthly for 12 months. RESULTS: Only 450 patients completed the 1-year follow up and were enrolled in our present analyses. Mean age was (65.2 +/- 10.6) years, men 309 (68.7%). The BODE index was categorized into 4 subgroups: 0 - 2, 3 - 4, 5 - 6 and 7 - 10. At baseline BODE index was gradually increased with baseline total SGRQ and SGRQ subscales (P trend < 0.001). For individual components of BODE index, with the decrease of airflow limitation, and 6MWD, and with the increase of Medical Research Council (MRC) dyspnea grade, total SGRQ and SGRQ subscales were increased correspondingly, P trend < 0.05, respectively. Similar association patterns were found between baseline BODE index and its individual components and mean SGRQ scores at the end of 1-year follow up. By multiple linear regression analyses, baseline BODE index was not only significantly associated with SGRQ score at baseline but also with SGRQ score at the end of 1-year follow up after adjustment for age, male, current smoking, betas being 0.434 and 0.378, respectively. CONCLUSIONS: BODE index is associated with SGRQ score cross-sectionally and longitudinally among stable COPD patients. BODE index might have potential to be used as a sensitive tool to assess the status of quality of life and to monitor disease progression among stable COPD patients.


Assuntos
Doença Pulmonar Obstrutiva Crônica/patologia , Doença Pulmonar Obstrutiva Crônica/fisiopatologia , Qualidade de Vida , Idoso , Índice de Massa Corporal , Estudos Transversais , Dispneia/patologia , Dispneia/fisiopatologia , Tolerância ao Exercício/fisiologia , Feminino , Humanos , Modelos Lineares , Estudos Longitudinais , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Testes de Função Respiratória , Fumar , Inquéritos e Questionários
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA