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1.
Int J Biol Macromol ; 2020 Mar 14.
Artigo em Inglês | MEDLINE | ID: mdl-32184138

RESUMO

Sustainable low-cost cellulose-based electronics has exhibited brighter prospects. In this study, a laminated felt-like electromagnetic shielding material was prepared by using cellulose paper as the matrix through the self-foaming effect in electroless nickel plating process in which an efficient palladium-free activation was conducted that alkaline sodium borohydride (NaBH4) reduced nickel ions into nickel cluster to initiate the plating process. NaOH concentration in NaBH4 solution, pH of the plating bath and the plating time affected the thickness, metal deposition and surface resistance of the electromagnetic shielding material. By understanding the morphology, inner structure, chemical component and thermal stability, the pH in the plating solution is a key for the preparation. When the pH was 8.51, the electromagnetic shielding effective reached 65 dB in the frequency ranges of 9 kHz to 1.5 GHz, which could shield more than 99.99% electromagnetic radiation. This work offers a novel and feasible path to develop functional cellulose-based materials.

2.
Artigo em Inglês | MEDLINE | ID: mdl-32162615

RESUMO

The transition sequence in the Heusler alloy Ni50Mn34In8Ga8 has been determined from measurements of elasticity, heat flow and magnetism to be paramagnetic austenite → paramagnetic martensite → ferromagnetic martensite at ~335 and ~260 K, respectively, during cooling. The overall pattern of elastic stiffening/softening and acoustic loss is typical of a system with bilinear coupling between symmetry breaking strain and the driving order parameter in a temperature interval below the transition point in which ferroelastic twin walls remain mobile under the influence of external stress. Divergence between zero-field-cooling (ZFC) and field-cooling (FC) determinations of DC magnetisation below ~220 K indicates that a frustrated magnetic glass develops in the ferromagnetic martensite. An AC magnetic anomaly which shows Vogel-Fulcher dynamics in the vicinity of ~160 K is evidence of a further glassy freezing process. This coincides with an acoustic loss peak and slight elastic stiffening that is typical of the outcome of freezing of ferroelastic twin walls. The results indicate that local strain variations associated with the ferroelastic twin walls couple with local moments to induce glassy magnetic behaviour.

3.
J Speech Lang Hear Res ; 63(3): 774-792, 2020 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-32163319

RESUMO

Purpose We compared the narrative production in Mandarin-speaking children at risk (AR) for developmental language disorder (DLD) and typically developing (TD) controls to address two goals: (a) further our understanding of the Mandarin DLD phenotype and (b) examine the role of elicitation method in differentiating AR from TD. Method Twenty-one AR children and 21 age- and nonverbal IQ-matched peers produced two stories from the Multilingual Assessment Instrument of Narrative, first following an adult model (i.e., story-retell) and then without a model (i.e., story-tell). Group and task effects were analyzed on macrostructure and microstructure measures. Results For general macrostructure score and sentence complexity, children in the AR group performed more poorly than TD children on the more challenging story-tell task and showed decreased scores from retell to tell tasks. In addition, children in the AR group showed poorer performance on number of different words. Productivity and grammaticality measures did not show group differences. Discussion Consistent with previous findings, grammaticality and productivity were relatively preserved but story macrostructure, lexical diversity, and sentence complexity were vulnerable in Mandarin-speaking children with or AR for DLD. Having an adult model benefited both groups in sentence complexity and story macrostructure and potentially helped maintain the performance in TD children as they engaged in the more challenging story-telling task.

4.
Dent Traumatol ; 2020 Mar 13.
Artigo em Inglês | MEDLINE | ID: mdl-32170848

RESUMO

BACKGROUND/AIMS: Traumatic dental injuries (TDIs) are considered to be a public dental health problem worldwide. The aim of the current study was to provide the worldwide tendency and perspectives in TDIs in the last two decades via bibliometric analysis. METHODS: ''Tooth injuries'' was searched as the Medical Subject Headings term within PubMed with the date range from 1999 to 2018. Two investigators perused information in the articles according to the inclusion and exclusion criteria. The articles were independently categorized according to the following aspects: i) annual scholarly output; ii) leading countries or regions; iii) leading journals; iv) productive authors; v) citations; vi) study design; vii) distribution of topics; and viii) the type of dentition and TDIs. VOSviewer 1.6.7 and Citespace 5.2 were used for analyzing and visualizing bibliometric networks. RESULTS: A total of 2627 articles about traumatic dental injuries were published and indexed in PubMed during the two decades, and the number of publications on traumatic dental injuries was rising in general. The research outputs were mainly concentrated in developed countries and affiliated hospitals of universities. Brazil was the most productive country. The journal Dental Traumatology had the most contributions to the scientific research of traumatic dental injuries. "Case report" was the most frequent type of article (36.50%), followed by cross-sectional studies (19.57%) and case-control studies (13.67%). Most studies focused on the treatment of TDIs (38.94%), especially for avulsion (21.01%), crown fracture (9.71%) and intrusion (5.25%). Permanent teeth (66%) was the dominant dentition. CONCLUSION: There is a lack of high quality well-designed studies such as cohort studies. The number of publications on prevention and the primary dentition is disproportionate in relation to their significance.

5.
Gene Ther ; 2020 Feb 07.
Artigo em Inglês | MEDLINE | ID: mdl-32034293

RESUMO

The transcription factor NF-κB is an attractive target for cancer therapy due to its over-activation in all tumours; however, NF-κB inhibitors developed in the past decades rarely became drugs due to undesirable side effects. In this study, we developed a gene therapy strategy named NF-κB-activated gene expression (Nage), which could induce the death of cancer cells in vitro and in vivo by utilising NF-κB over-activity in cancer cells, but had no effects on normal cells. Nage was consisted of an NF-κB-specific promoter formed by fusing an NF-κB decoy sequence with a minimal promoter, which could be bound by the intracellular over-activated NF-κB and thus activated the expression of downstream effector genes in an NF-κB-specific manner. In this study, we first confirmed the cancer cell-specific over-activation of NF-κB and then tested the cancer cell specificity of the Nage vector by expressing the reporter gene ZsGreen in various in vitro cultivated cells. We next demonstrated that the Nage vector could be used to express CRISPR/Cas9 protein only in cancer cells. The Cas9 protein was then guided by a sgRNA targeting telomeric DNA and induced cancer cell death. The Nage vector expressing Cas9/sgRNA could be packaged into adeno-associated virus (AAV) and intravenously injected to inhibit tumour growth in mice without visible side effects and toxicity.

6.
Sci Rep ; 10(1): 3469, 2020 Feb 26.
Artigo em Inglês | MEDLINE | ID: mdl-32103046

RESUMO

To effectively control soil erosion, three hierarchies of the National Soil and Water Conservation Regionalization Scheme have been established in China. However, the scheme has its limits, which can be summarized by two points: first, the tertiary hierarchy functional region exhibits obvious heterogeneity; second, the ecosystem function does not influence the regionalization scheme results during the process of regionalization. To enhance the guidance of the regionalization, a new indicator system included soil erosion risk, soil erosion intensity and ecosystem service value was developed to explore the subdivision of the tertiary hierarchy functional region. Moreover a scheme for the subdivision of the tertiary hierarchy functional region was formed. In this scheme, the central Hunan hilly soil conservation and living environmental protection section was divided into three subregions: Luoxiao-Xuefeng Mountain high ecological value section, Xiangjiang middle and downstream medium ecological value section, and Hengyang Basin low ecological value section. Specifically, with regard to soil and water conservation regionalization, the concept of subregions within the tertiary hierarchy-based functional region was proposed and the new indicator system that highlighted ecosystem functions was applied for the first time on a regional scales; this method provides a new way of thinking about other regionalization schemes.

7.
Med Hypotheses ; 139: 109634, 2020 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-32087491

RESUMO

Toxic insult to the gonads by chemotherapy or radiotherapy can lead to permanent infertility. It's an important health concern because each year more than 4000 male patients are at risk of azoospermia in the United States due to gonadotoxicity of the regimens used. There are also several benign/genetic diseases whose natural course can result in infertility without gonadotoxic therapy. Considering the fact that most of these people are cured and survive with the advent of modern medicine, infertility is related to serious psychological and relationship implications and parenthood is a significant issue for those patients. Semen cryopreservation option is available for postpubertal adolescent and adult men, while children do not have this storing option since they do not have mature spermatozoa. However, their testes contain spermatogonial stem cells (SSCs), which are initiators of spermatogenesis. Promising findings in animal studies and human cell lines have encouraged scientists that SSCs may be hope for restoring fertility option of patients who cannot produce functional sperm and who have no other choice to preserve their future fertility. For this reason, several centers around the world already began to collect and cryopreserve testicular tissue or cells with anticipation that SSC-based therapies will be available in the near future; however, an optimal transplantation design in humans is yet to be developed. Here we propose an allogeneic testicular stem cell transplantation with an encapsulation device to restore fertility in patients with infertility. We endeavor to discuss the reliability of this method with the current literature and bring the evidence on its feasibility.

8.
J Periodontal Res ; 2020 Jan 20.
Artigo em Inglês | MEDLINE | ID: mdl-31960448

RESUMO

OBJECTIVES: This trial aimed to evaluate the efficacy and safety of vitamin D supplementation on the residual moderate and deep pockets following nonsurgical periodontal therapy. BACKGROUND: Vitamin D supplementation has potential effects on periodontitis, but current evidence remains inconclusive. METHODS: After 3 months of nonsurgical periodontal treatment, 360 patients with moderate or severe periodontitis were randomly assigned to 2000 international unit (IU)/d vitamin D3, 1000 IU/d vitamin D3, or placebo. Clinical periodontal examinations, including probing depth (PD), bleeding index (BI), plaque index (PLI), attachment loss (AL), and alveolar crest height (ACH), were performed at baseline and after 3 months of intervention. RESULTS: There was a slight but significant decrease in AL and PD in both vitamin D groups compared with placebo group for moderate and deep pockets. About 2000 IU/d vitamin D3 group, 1000 IU/d vitamin D3 group, and placebo group all decreased the AL for both moderate pockets (-0.4 mm vs -0.4 mm vs -0.3 mm) and deep pockets (-1.1 mm vs -1.1 mm vs -1.0 mm) (all P < .05). Similarly, PD was also decreased in these three groups for both moderate pockets and deep pockets (all P < .05). In addition, vitamin D supplementation was well tolerated, and no adverse events were reported. CONCLUSIONS: Although statistically significant differences were observed in favor to vitamin D supplementation, the magnitude of effect size tended to be modest with limited clinical relevance and the long-term efficacy and safety warrant further investigation.

9.
Mol Immunol ; 117: 110-121, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31765840

RESUMO

Although granulocyte colony-stimulating factor(G-CSF) has pathogenic roles in several immune inflammatory diseases, its role in periodontitis has not been investigated. Here we detected local expression of G-CSF using public datasets in the Gene Expression Omnibus (GEO) database, and immune cell infiltration into gingival tissue was estimated based on single-sample gene set enrichment analysis (ssGSEA). G-CSF expression and neutrophil infiltration were also confirmed by human gingival biopsies analysis. Moreover, anti-G-CSF neutralizing antibody was locally administrated to investigate the effects of G-CSF neutralization on neutrophils infiltration and periodontal tissue destruction in periodontitis mice model. Two public datasets (GSE10334 and GSE16134), which included 424 patients with periodontitis and 133 health controls, were used in the analysis. Markedly increased immune cell infiltration and G-CSF expression in gingival tissues were found in the periodontitis group as compared to the control group. The higher expression of G-CSF was correlated with higher infiltration of immune cells, especially with neutrophil infiltration. Analysis of gingival biopsies further confirmed high neutrophil infiltration and G-CSF expression. In addition, anti-G-CSF antibody-treated mice with periodontitis showed significantly reduced alveolar bone resorption and neutrophil infiltration when compared with periodontitis mice treated with isotype control antibody. Also, anti-G-CSF antibody treatment significantly reduced mRNA expression of CXC chemokines (CXCL1, CXCL2 and CXCL3), interleukin 1ß (IL-1ß), IL-6, matrix metalloproteinases 9, receptor activator of nuclear factor κB ligand/osteoprotegerin (RANKL/OPG) ratio and osteoclasts number in periodontal tissues. In summary, neutrophil infiltration and G-CSF expression levels were significantly increased in inflamed gingival tissues. G-CSF neutralization in periodontal inflammation could alleviate neutrophil infiltration and periodontal tissue destruction in experimental periodontitis.

10.
Mater Sci Eng C Mater Biol Appl ; 106: 110302, 2020 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-31753337

RESUMO

Nanotherapeutics and nanopharmaceuticals could achieve and facilitate earlier and more precise individual diagnosis, improve targeted therapies, reduce side effects, and enhance therapeutic monitoring. These advantages will improve quality of life, support a healthier and more independent aging population, and be instrumental in maximizing the cost-effectiveness of health care. However, the field of nanomedicine is at its early stage, most of the research still stays in the laboratory phase, and few success stories are translated into clinical trials and medical practice. This review will demonstrate the numerous challenges that are encountered during the development of commercial nanoparticle-based therapeutics and the possible solutions.

11.
Nano Lett ; 20(2): 1262-1271, 2020 Feb 12.
Artigo em Inglês | MEDLINE | ID: mdl-31877053

RESUMO

Conventional refrigeration methods based on compression-expansion cycles of greenhouse gases are environmentally threatening and cannot be miniaturized. Electrocaloric effects driven by electric fields are especially well suited for implementation of built-in cooling in portable electronic devices. However, most known electrocaloric materials present poor cooling performances near room temperature, contain toxic substances, and require high electric fields. Here, we show that lead-free ferroelectric thin-film bilayers composed of (Bi0.5Na0.5)TiO3-BaTiO3 (BNBT) and Ba(Zr0.2Ti0.8)O3-(Ba0.7Ca0.3)TiO3 (BCZT) display unprecedentedly large electrocaloric effects of ∼23 K near room temperature under moderate electric bias. The giant electrocaloric effect observed in BNBT/BCZT bilayers, which largely surpasses the sum of the individual caloric responses measured in BNBT and BCZT, is originated from the presence of compositional bound charges at their interface. Our discovery of interface charge-induced giant electrocaloric effects indicates that multilayered oxide heterostructures hold tremendous promise for developing highly efficient and scalable solid-state cooling applications.

12.
Zhonghua Wei Zhong Bing Ji Jiu Yi Xue ; 31(9): 1167-1169, 2019 Sep.
Artigo em Chinês | MEDLINE | ID: mdl-31657347

RESUMO

OBJECTIVE: Metabolic reprogramming is the response of cells to environmental changes, such as cell activation, proliferation and differentiation, which involves changes in metabolism-related enzymes, metabolites and metabolic pathways. Sepsis-associated immune cells undergo metabolic reprogramming in response to inflammatory signals, which not only provides biological energy and biosynthesis requirements, but also determines cell fate and function in a highly specific way. In this paper, the changes in glycolysis, tricarboxylic acid cycle, oxidative phosphorylation and other glucose metabolism pathways of macrophages, T lymphocytes, dendritic cells, neutrophils and other sepsis related immune cells are described, so as to provide feasibility for future research and metabolic therapy.


Assuntos
Glicólise , Sepse , Células Dendríticas , Glicogênio , Humanos , Fosforilação Oxidativa
13.
Vet Microbiol ; 237: 108403, 2019 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-31585656

RESUMO

Classical swine fever (CSF) still threatens the swine industry in China, with genotype 2 isolates of CSFV dominating the epizootics. In 2018 the first E2 subunit marker vaccine against CSFV (Tian Wen Jing, TWJ-E2®), containing a baculovirus-expressed E2 glycoprotein of a genotype 1.1 vaccine strain, was officially licensed in China and commercialized. To evaluate the cross-protective efficacy of TWJ-E2 against different virulent genotype 2 Chinese field isolates (2.1b, 2.1c, 2.1 h, and 2.2), 4-week-old pigs were immunized with the TWJ-E2 vaccine according to the manufacturer's instructions and then challenged with genotype 2 strains. A group vaccinated with the conventional C-strain vaccine was included for comparison. TWJ-E2 vaccinated pigs developed higher levels of E2 and neutralizing antibodies than those receiving the commercial C-strain vaccine. All TWJ-E2 and C-strain vaccinated pigs survived challenge without development of fever, clinical signs or pathological lesions. In contrast, all unvaccinated control pigs displayed severe CSF disease with 40-100% mortalities by 24 days post challenge. None of the TWJ-E2 and C-strain vaccinated pigs developed viremia, viral shedding from tonsils, Erns protein in the sera, or viral RNA loads in different tissues after challenge, all of which were detected in the challenged unvaccinated controls. We conclude that vaccination of young pigs with TWJ-E2 provides complete immune protection against genotypically heterologous CSFVs and prevents viral shedding after challenge, with an efficacy at least comparable to that elicited by the conventional C-strain vaccine.


Assuntos
Vírus da Febre Suína Clássica/genética , Peste Suína Clássica/prevenção & controle , Genótipo , Proteínas do Envelope Viral/imunologia , Vacinas Virais/imunologia , Animais , Anticorpos Neutralizantes/genética , Anticorpos Antivirais/sangue , Subunidades Proteicas/imunologia , Suínos , Vacinas de Subunidades
14.
Biol Trace Elem Res ; 2019 Sep 09.
Artigo em Inglês | MEDLINE | ID: mdl-31502179

RESUMO

The human placenta is an important organ that forms a barrier where maternal and fetal exchange takes place. The placenta transport iodine to the fetal circulation by transfer of maternal iodine and deiodination of thyroid hormones (THs). The aim of the study was to examine the distribution of iodine and thyroid hormone transporters in the maternal and fetal sides of human-term placenta. A cross-sectional study was performed at the First Affiliated Hospital of China Medical University. Placental samples (maternal and fetal surfaces) were collected from 113 healthy-term pregnant women. The iodine content; the concentration of thyroxine (T4), triiodothyronine (T3), and reverse T3 (rT3); and the enzyme activity of placental type 2 iodothyronine deiodinase (D2) and D3 were examined. The mRNA and protein localization/expression of iodine and thyroid hormone transporters in the placenta were also studied. We also analyzed the association between expression level of Na+/I- symporter (NIS), thyroid hormone transporter protein, D3 activity in maternal and fetal surfaces of placenta with iodine content, and thyroid hormone levels. Iodine levels in placental samples from the maternal side were significantly higher than those in samples from the fetal side. T3 and T4 expression in fetal placenta was significantly lower than in maternal placenta. D3 activity in the fetal side of the placentas was significantly higher than that in the maternal side. The mRNA and protein expression of monocarboxylate transporters 8 (MCT8), L-amino acid transporters 1 (LAT1), organic anion transporting polypeptides 4A1 (OATP4A1), and TH binding protein transthyretin (TTR) were significantly increased in maternal side, while the NIS expression was higher in fetal side of human-term placenta. In conclusion, the enzymatic deiodination of thyroid hormones forms a barrier which reduces transplacental passage of the hormones and that the maternal part of the placenta is the primary factor in the mechanism regulating the hormonal transfer.

15.
Acc Chem Res ; 52(8): 2290-2300, 2019 Aug 20.
Artigo em Inglês | MEDLINE | ID: mdl-31386341

RESUMO

Lithium-ion batteries have received significant attention over the last decades due to the wide application of portable electronics and increasing deployment of electric vehicles. In order to further increase the energy density of batteries and overcome the capacity limitations (<250 mAh g-1) of inorganic cathode materials, it is imperative to explore new cathode materials for rechargeable lithium batteries. Organic compounds including organic carbonyl, radicals, and organosulfides are promising as they have advantages of high capacities, abundant resources, and tunable structures. In the 1980s, a few organosulfides, in particular organodisulfides, as cathode materials were studied to a certain extent in rechargeable lithium batteries. However, they showed low capacities and poor cycling performance, which made them unappealing then in comparison to transition metal oxide cathode materials. As a result, organosulfides have not been extensively studied like other cathode materials including organic carbonyls and radicals. In recent years, organosulfides with long sulfur chains (e.g., trisulfide, tetrasulfide, pentasulfide, etc.) in the structures have been receiving more attention in conjunction with the development of lithium-sulfur batteries. As a major class of sulfur derivatives, they have versatile structures and unique properties in comparison with elemental sulfur. In this Account, we first generalize the working principles of organosulfides in lithium batteries. We then discuss organosulfide molecules, which have precise lithiation sites and tunable capacities. The organic functional groups can provide additional benefits, such as discharge voltage and energy efficiency enhancement by phenyl groups and cycling stability improvement by N-heterocycles. Furthermore, replacing sulfur by selenium in these compounds can improve their electrochemical properties due to the high electronic conductivity and low bond energy associated with selenium. We list organosulfide polymers consisting of phenyl rings, N-heterocycles, or aliphatic segments. Organosulfides as electrolyte additives or components for forming a solid-electrolyte interphase layer on lithium metal anode are also presented. Carbon materials such as carbon nanotubes and reduced graphene oxide can enhance the battery performance of organosulfide cathodes. We discuss the synthesis methods for polysulfide molecules and polymers. Finally, we show the advantages of organosulfides over sulfur as cathode materials in lithium batteries. This Account provides a summary of recent development, in-depth analysis of structure-performance relationship, and guidance for future development of organosulfides as promising cathode materials for next generation rechargeable lithium batteries.

16.
Immunol Lett ; 213: 1-8, 2019 09.
Artigo em Inglês | MEDLINE | ID: mdl-31295429

RESUMO

Adjuvants can improve the efficacy of influenza vaccines and are still a hot spot in the study and development of influenza vaccines. In this report, the effects of aqueous extracts of Cistanche deserticola (AECD) as a polysaccharide adjuvant on seasonal influenza vaccines (IVV) were explored. The experimental data of anti-IVV IgG1 and IgG as well as hemagglutinin inhibition (HI) titers in young adult mice indicated that IVV intramuscularly co-injected with AECD was significantly more immunogenic than alum-adjuvanted or non-adjuvanted IVV. AECD-adjuvanted vaccine could rapidly initiate specific IgG response. Similarly, IVV with AECD augmented significantly lymphocyte proliferation and increased the positive rates of CD4+, CD8+ and CD44+ T cells from draining lymph nodes and spleens. Importantly, IVV with AECD could induce the Th1 immune response, as indicated by higher IgG2a levels accompanied by the induction of IFN-γ in CD4+ and CD8+ T cells. Additionally, IVV with AECD activated dendritic cells (DCs) and decreased the expression of Treg cells. There were no noticeable side effects after the vaccination. In brief, the addition of AECD enhanced immunogenicity to seasonal influenza vaccine by the induction of HI antibody generation, more rapid humoral immune responses, and a balanced Th1-/Th2-type response, effective T-cell responses, which may be important for seasonal influenza vaccines with broad and long lasting immunity.

17.
Food Sci Biotechnol ; 28(4): 1065-1072, 2019 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-31275706

RESUMO

In this study microwave and far-infrared combination drying were conducted to investigate the effect of microwave and far-infrared heating mode switching point water content (SW), ginseng slice thickness, and far-infrared drying temperature on drying indicators (surface colour difference, ginsenosides content, and surface shrinkage rate) and drying efficiency (drying time) during the process of drying white ginseng slices. Regarding microwave drying, the microwave drying time cannot exceed 150 s, and the ginseng slice water content cannot be less 50%. For the combination drying, SW, far-infrared drying temperature and slice thickness increased, the colour difference and surface shrinkage rate first decreased and then increased, and the content of ginsenosides first increased and then decreased. In addition, the combination drying showed faster drying rate, higher ginsenosides contents value, colour difference (ΔE) value and lower surface shrinkage rate than single far-infrared drying.

18.
Artigo em Inglês | MEDLINE | ID: mdl-31234521

RESUMO

Industrial pollution control is a difficult problem in China's current economic transformation, and the Chinese government has implemented many measures to deal with it. However, little research has focused on the relationship between land policy and industrial pollution. Based on the theoretical discussion of the mechanism influencing the market-oriented reform of industrial land (mainly refer to the marketization of land conveyance price and the openness of land conveyance process) on urban industrial pollution, we constructed an analytical framework by linking land policy with industrial pollution. Then, we constructed an econometric model and chose the statistical data of 104 large- and medium-sized cities in mainland China from 2003 to 2016. The results indicate that with the marketization of the industrial land conveyance price, urban industrial pollution is presenting an inverted U-shaped change trend. For cities in different development stages of industrialization, there is no difference in the impact of industrial land conveyance price on urban industrial pollution. However, the openness of industrial land conveyance promotes and inhibits the urban industrial pollution in the stages of industrialization and post-industrialization, respectively. Finally, this paper puts forward some suggestions on how to control industrial pollution from the perspective of further improving the industrial land conveyance mechanism.


Assuntos
Poluição Ambiental/prevenção & controle , Desenvolvimento Industrial , Modelos Econométricos , China , Cidades , Comércio , Indústrias
19.
J BUON ; 24(2): 853-858, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31128046

RESUMO

PURPOSE: Plant metabolites have gained considerable attention as the anticancer agents over the last few decades. Previous studies have indicated the potential of taxifolin as an anticancer agent. However, the information on the anticancer activity of taxifolin against skin scar cell carcinoma as well as several other types of cancers is scantly. Against this background, the present study was designed to investigate the anticancer activity of taxifolin against a panel of skin scar cell carcinoma cell lines. MATERIALS AND METHODS: Proliferation rate of the cells was monitored by MTT assay. DAPI and annexin V/PI assay was used to investigate the induction of apoptosis. Flow cytometery was employed to carry out the cell cycle analysis. Transwell assay was used to check the invasion of the cancerous cells. RESULTS: The results indicated that taxifolin inhibits the growth of the skin scar cell carcinoma cell lines. However the anticancer effects were more profound on the SSCC cancer cells (IC50, 20 µM). In contrast the anticancer effects of taxifolin on the non-cancerous skin cells were minimal. Further investigation revealed that the anticancer effects of taxifolin on the SSCC cells is due to the induction of apoptosis and cell cycle arrest. Moreover, taxifolin could also inhibit the invasion of SSCC cells which was associated with downregulation of the MMP-2 and MMP-9 expression indicative of the potential of taxifolin in the treatment of scar cell carcinoma Conclusion: It is was found that taxifolin inhibited the growth of skin scar cell carcinoma growth by triggering apoptosis and cell cycle arrest and also inhibited cell invasion capacity and therefore this study warrants further investigation on the anticancerous potential of taxifolin.


Assuntos
Carcinoma/tratamento farmacológico , Proliferação de Células/efeitos dos fármacos , Quercetina/análogos & derivados , Neoplasias Cutâneas/tratamento farmacológico , Apoptose/efeitos dos fármacos , Carcinoma/genética , Carcinoma/patologia , Pontos de Checagem do Ciclo Celular/efeitos dos fármacos , Linhagem Celular Tumoral , Cicatriz/patologia , Citometria de Fluxo , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Metaloproteinase 2 da Matriz/genética , Metaloproteinase 9 da Matriz/genética , Fosfatidilinositol 3-Quinases/genética , Proteínas Proto-Oncogênicas c-akt/genética , Quercetina/farmacologia , Transdução de Sinais/efeitos dos fármacos , Neoplasias Cutâneas/genética , Neoplasias Cutâneas/patologia , Serina-Treonina Quinases TOR/genética
20.
Elife ; 82019 04 11.
Artigo em Inglês | MEDLINE | ID: mdl-30973327

RESUMO

The deactivated CRISPR/Cas9 (dCas9) is now the most widely used gene activator. However, current dCas9-based gene activators are still limited by their unsatisfactory activity. In this study, we developed a new strategy, the CRISPR-assisted trans enhancer, for activating gene expression at high efficiency by combining dCas9-VP64/sgRNA with the widely used strong CMV enhancer. In this strategy, CMV enhancer DNA was recruited to target genes in trans by two systems: dCas9-VP64/csgRNA-sCMV and dCas9-VP64-GAL4/sgRNA-UAS-CMV. The former recruited trans enhancer by annealing between two short complementary oligonucleotides at the ends of the sgRNA and trans enhancer. The latter recruited trans enhancer by binding between GAL4 fused to dCas9 and UAS sequence of trans enhancer. The trans enhancer activated gene transcription as the natural looped cis enhancer. The trans enhancer could activate both exogenous reporter genes and variant endogenous genes in various cells, with much higher activation efficiency than that of current dCas9 activators.

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